Details of a Researcher - Okano, Hideyuki

Papers - Okano, Hideyuki

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Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis

Kawashima Takeshi, Murakami Akikazu R., Ogasawara Michio, Tanaka Kimio J., Isoda Ryuji, Sasakura Yasunori, Nishikata Takahito, Okano Hideyuki, Makabe Kazuhiro W.

Development Genes and Evolution 210 ( 3 ) 162 - 165 2000

ISSN 0949-944X

The gene family encoding RNA-binding proteins includes important regulators involved in the neurogenesis in both protostomes and deuterostomes. We isolated cDNAs of the ascidian homolog of one of the RNA-binding proteins, MUSASHI, from Halocynthia roretzi and Ciona intestinalis. The predicted amino acid sequences contained two RNA-recognition and RNA-binding motifs in the N-terminus and an ascidian-specific YG-rich domain in the C-terminus. Maternal transcripts of musashi were ubiquitous in early cleavage-stage embryos. Ascidian musashi had three domains of zygotic expression: the brain, nerve cord, and mesenchyma. The temporal order of the onset in these domains was highly divergent between the two species of ascidian examined.

Identification and selection of neural progenitor cells

Okano H., Goldman S. A.

NeuroScience News 3 ( 6 ) 27 - 31 2000

ISSN 1027-6599

Neural stem cells (NSCs) are self-renewing and multipotential neural progenitor cells, which have received strong attention as potential tools to treat the injured or diseased brain. To develop therapeutic strategies intended to capitalize upon the plasticity and propagability of NSCs, we need both to better understand their biology, and to develop better means for their prospective identification and harvest. We summerize here recent findings from our laboratories. Specifically, we describe the prospective identification of NSCs, using fluorescent reporters placed under the control of the nestin enhancer. Together, these findings provide us both new conceptual and operational strategies for studying NSCs, and as such should greatly accelerate their development for the treatment of neurologic disease.

MSI-1, a neural RNA-binding protein, is involved in male mating behaviour in Caenorhabditis elegans

Yoda Akinori, Sawa Hitoshi, Okano Hideyuki

Genes to Cells 5 ( 11 ) 885 - 895 2000

ISSN 1356-9597

Background: Neural RNA-binding proteins are thought to play important roles in neural development and the functional regulation of postmitotic neurones by mediating post-transcriptional gene regulation. RNA-binding proteins belonging to the Musashi family are highly expressed in the nervous system; however, their roles are poorly understood. Results: We identified a Caenorhabditis elegans Musashi homologue, MSI-1, whose RNA-recognition motifs show extensive similarity to those of Drosophila and vertebrate Musashi proteins. We isolated a msi-1 mutant and found males with this mutation to have a mating defect. C. elegans male mating behaviour includes a distinct series of steps: response to contact, backing, turning, vulva location, spicule insertion, and sperm transfer. msi-1 is required for the turning and vulva location steps. Like other Musashi family members, MSI-1 is expressed specifically in neural cells, including male-specific neurones required for turning and vulva location. However, msi-1 was not expressed in proliferating neural progenitors in C. elegans, unlike the Musashi family genes in other systems. Conclusions: Our results suggest that msi-1 is expressed specifically in postmitotic neurones in C. elegans. msi-1 is required for full development of male mating behaviour, possibly through regulation of msi-1 expressing neurones.

Access to Document (DOI)

Components of the SWI/SNF complex are required for asymmetric cell division in C. elegans

Sawa Hitoshi, Kouike Hiroko, Okano Hideyuki

Molecular Cell 6 ( 3 ) 617 - 624 2000

ISSN 1097-2765

Asymmetric cell division is a fundamental process that produces cellular diversity during development. We have identified two mutants in C. elegans (psa-1 and psa-4) in which the asymmetry of T cell division is disrupted. psa-1 and psa-4 encode homologs of yeast SW13 and SW12/SNF2, respectively, which are components of the SWI/SNF complex. We show by RNA interference assay that homologs of other components of SWI/SNF are also involved in T cell division. psa-1 and psa-4 are likely to be required in the T cell during mitosis to cause asymmetric cell division. Because the SWI/SNF complex is required for asymmetric division in S. cerevisiae, these results demonstrate that at least some aspects of the mechanism of asymmetric cell division are conserved between yeast and a multicellular organism.

Neural stem cells

Okano Hideyuki

Clinical Neurology 41 ( 12 ) 1036 - 1040 2001.12

ISSN 0009-918X

Neural stem cells (NSCs) are multipotential progenitor cells that have self-renewal activities. A single NSC is capable of generating various kinds of cells within the CNS, including neurons, astrocytes, and oligodendrocytes. Because of these characteristics, there is an increasing interest in NSCs and neural progenitor cells from the aspects of both basic developmental biology and therapeutic applications to the damaged brain. By understanding of nature of NSCs present in CNS, extracellular factors and signal transduction cascades involved in the differentiation and maintenance of NSCs, population dynamics and localizations of NSCs in embryonic and adult brains, prospective identification and isolation of NSCs, and induction of NSCs into particular neuronal phenotypes, which will be introduced in this review, it would be possible to develop a feasible strategy to manipulate cells in situ to treat the damaged brain.

In vitro selection法によるRNA結合タンパク質の標的配列の同定

Imai Takao，Okano Jiimusuhirotaka，Okano Hideyuki

細胞工学 20 ( 12 ) 1690-1697 2001.12

Research paper (scientific journal), Joint Work

パーキンソン病治療への新たな布石

Sawamoto Kazunobu，Okano Hideyuki

Bio ベンチャー 1 ( 3 ) 90-91 2001.11

Research paper (scientific journal), Joint Work

Role of Deltex-1 as a transcriptional regulator downstream of the Notch receptor

Yamamoto Naoya, Yamamoto Shin-ichi, Inagaki Fuyuki, Kawaichi Masashi, Fukamizu Akiyoshi, Kishi Noriyuki, Matsuno Kenji, Nakamura Kozo, Weinmaster Gerry, Okano Hideyuki, Nakafuku Masato

Journal of Biological Chemistry 276 ( 48 ) 45031-45040 2001.11

Research paper (scientific journal), Joint Work

再生治療の海外基礎研究開発状況―特に幹細胞生物学研究を中心に

Kanemura Yonehiro，Okano Hideyuki

Science&Technology Jounal 10 ( 00 ) 14-17 2001.11

Research paper (scientific journal), Joint Work

神経幹細胞の同定・分離の確立と神経再生への挑戦

Okano Hideyuki

神経治療学 18 ( 5/6合併号 ) 443-449 2001.11

Research paper (scientific journal), Single Work

神経幹細胞と再生

Imai Takao，Okano Jiimusuhirotaka，Okano Hideyuki

治療学 35 29-35 2001.10

Research paper (scientific journal), Joint Work

RNA-binding protein Musashi2: Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS

Sakakibara Shin-ich, Nakamura Yuki, Satoh Hitoshi, Okano Hideyuki

Journal of Neuroscince 21 ( 20 ) 8091-8107 2001.10

Research paper (scientific journal), Joint Work

再生医学の基本戦略：中枢神経系を中心として

Okano Hideyuki

KAST Report 13 ( 1 ) 24-29 2001.10

Research paper (scientific journal), Single Work

神経幹細胞：その基礎生物学と高次神経機能・精神神経疾患への応用

Okano Hideyuki

精神神経学雑誌 103 ( 10 ) 759-763 2001.10

Research paper (scientific journal), Single Work

幹細胞システムを用いた中枢神経系の再生医学

Okano Hideyuki

Neurologia medico-chirurgica 41 ( extra issue ) 23 2001.10

Research paper (scientific journal), Single Work

Brain from bone:Efficient'meta-differentiation'of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent

Jun Kohyama, Hitoshi Abe, Takuya Shimazaki, Amane Koizumi, Kin-ichi Nakashima, Satoshi Gojo, Tetsuya Taga, Hideyuki Okano, Jun-ichi Hata, Akihiro Umezawa

Differentiation 68 ( 4-5 ) 235-244 2001.10

Research paper (scientific journal), Joint Work

IP₃ receptor type 1 mRNAの3'末端非翻訳領域に結合する遺伝子産物と小脳purkinje細胞におけるmRNA局在化機構

Iijima Takatoshi，Imai Takao，Kimura Yuuki，Okano Hideyuki

神経化学 40 ( 2,3 (Suppl) ) 311 2001.09

Research paper (scientific journal), Joint Work

An imaging-based 'time-table' of melecular expression in cultured murine neuroepthelial cells

Kawaguchi Ayano, Ogawa Maaharu, Okano Hideyuki, Saito Kaneko, Miyata Takayuki

Neurosciene Research 25 ( Suppl ) S128 2001.09

Research paper (scientific journal), Joint Work

High-yield selection and extraction of two promoter-defined phenotypes of neural stem cells from the fetal human brain

Keyoung Michael H, Roy Neeta S, Benraiss Abdellatif, Louissaint Abner Jr, Suzuki Akira, Hashimoto Mitsuhiro, Rashbaum William K, Okano Hideyuki, Goldman Steven A

Nature Biotechnology 19 ( 9 ) 843-850 2001.09

Research paper (scientific journal), Joint Work

Asymmetric inheritance of radial glial fibers by cortical neurons

Miyata Takaki, Kawaguchi Ayano, Okano Hideyuki, Ogawa Masaharu

Neuron 31 ( 5 ) 727-741 2001.09

Research paper (scientific journal), Joint Work

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