Papers - Okano, Hideyuki

Application of ES/somatic cell cloning technique to the production of genetically modified marmosets

Sotomaru, Yusuke, Sasaki, Erika, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E41 - E41 2011

Research paper (scientific journal),  ISSN  0168-0102

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Sensory network formation with RNA binding protein Musashi2

Shibata, Shinsuke, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E225 - E225 2011

Research paper (scientific journal),  ISSN  0168-0102

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Regulation of Posttranscriptional Modification as a Possible Therapeutic Approach for Retinal Neuroprotection

Ozawa, Yoko, Kurihara, Toshihide, Tsubota, Kazuo, Okano, Hideyuki

JOURNAL OF OPHTHALMOLOGY    1702 - 1710 2011

Research paper (scientific journal),  ISSN  2090-004X

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The maintenance of Purkinje axons by an RNA-binding protein HuC in adult cerebellum

Kakumoto, Kyoko, Miyazaki, Taisuke, Watanabe, Masahiko, Darnell, Robert, Okano, Hirotaka, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E95 - E96 2011

Research paper (scientific journal),  ISSN  0168-0102

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Transgenic technologies in nonhuman primates

Sasaki, Erika, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E41 - E41 2011

Research paper (scientific journal),  ISSN  0168-0102

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Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α

Houlihan Diarmaid D., Mabuchi Yo, Morikawa Satoru, Niibe Kunimichi, Araki Daisuke, Suzuki Sadafumi, Okano Hideyuki, Matsuzaki Yumi

Nature Protocols 7 ( 12 ) 2103 - 2111 2012.12

ISSN  1754-2189

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<p>Platelet-derived growth factor receptor α (PDGFR-α) and stem cell antigen 1 (Sca-1) have recently been identified as selective markers of mouse mesenchymal stem cells (MSCs). PDGFR-α + Sca-1 + (PαS) MSCs have augmented growth potential and robust tri-lineage differentiation compared with standard culture-selected MSCs. In addition, the selective isolation of PαS MSCs avoids cellular contamination that can complicate other methods. Here we describe in detail our protocol to isolate PαS MSCs using flow cytometry. In brief, the tibia and femora are isolated and crushed using a pestle and mortar. The crushed bones are then chopped and incubated for 1 h at 37°C in 20 ml of DMEM containing 0.2% (wt/vol) collagenase. The cell suspension is filtered before red blood cell lysis and incubated with the following antibodies: allophycocyanin (APC)-conjugated PDGFR-α, FITC-conjugated Sca-1, phycoerythrin (PE)-conjugated CD45 and Ter119. Appropriate gates are constructed on a cell sorter to exclude dead cells and lineage (CD45 + Ter-119 +)-positive cells. Approximately 10,000 PαS MSCs may then be isolated per mouse. The total protocol takes ∼7 h to complete. © 2012 Nature America, Inc.</p>

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Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-alpha

Houlihan, Diarmaid D., Mabuchi, Yo, Morikawa, Satoru, Niibe, Kunimichi, Araki, Daisuke, Suzuki, Sadafumi, Okano, Hideyuki, Matsuzaki, Yumi

NATURE PROTOCOLS 7 ( 12 ) 2103 - 2111 2012.12

Research paper (scientific journal),  ISSN  1754-2189

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The common marmoset as a novel animal model system for biomedical and neuroscience research applications

Okano, Hideyuki, Hikishima, Keigo, Iriki, Atsushi, Sasaki, Erika

SEMINARS IN FETAL &amp; NEONATAL MEDICINE 17 ( 6 ) 336 - 340 2012.12

Research paper (scientific journal),  ISSN  1744-165X

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Balanced ubiquitination determines cellular responsiveness to extracellular stimuli

Mukai, Akiko, Yamamoto-Hino, Miki, Komada, Masayuki, Okano, Hideyuki, Goto, Satoshi

CELLULAR AND MOLECULAR LIFE SCIENCES 69 ( 23 ) 4007 - 4016 2012.12

Research paper (scientific journal),  ISSN  1420-682X

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Conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography: Preclinical longitudinal study in common marmosets

Konomi, Tsunehiko, Fujiyoshi, Kanehiro, Hikishima, Keigo, Komaki, Yuji, Tsuji, Osahiko, Okano, Hirotaka James, Toyama, Yoshiaki, Okano, Hideyuki, Nakamura, Masaya

NEUROIMAGE 63 ( 4 ) 1841 - 1853 2012.12

Research paper (scientific journal),  ISSN  1053-8119

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Comparative gene expression in developing cognitive circuits in common marmoset brain

Mashiko, Hiromi, Yoshida, Aya C., Kikuchi, Satomi S., Niimi, Kimie, Takahashi, Eiki, Aruga, Jun, Okano, Hideyuki, Shimogori, Tomomi

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE 30 ( 8 ) 669 - 669 2012.12

Research paper (scientific journal),  ISSN  0736-5748

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Novel In Vivo Imaging Analysis of an Inner Ear Drug Delivery System in Mice: Comparison of Inner Ear Drug Concentrations over Time after Transtympanic and Systemic Injections

Kanzaki, Sho, Fujioka, Masato, Yasuda, Akimasa, Shibata, Shinsuke, Nakamura, Masaya, Okano, Hirotaka James, Ogawa, Kaoru, Okano, Hideyuki

PLOS ONE 7 ( 12 ) 163 - 175 2012.12

Research paper (scientific journal),  ISSN  1932-6203

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Comparative Anatomy of Marmoset and Mouse Cortex from Genomic Expression (April, pg 5039, 2012)

Mashiko, Hiromi, Yoshida, Aya C., Kikuchi, Satomi S., Niimi, Kimie, Takahashi, Eiki, Aruga, Jun, Okano, Hideyuki, Shimogori, Tomomi

JOURNAL OF NEUROSCIENCE 32 ( 49 ) 17932 - 17932 2012.12

Research paper (scientific journal),  ISSN  0270-6474

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Pre-Evaluated Safe Human iPSC-Derived Neural Stem Cells Promote Functional Recovery after Spinal Cord Injury in Common Marmoset without Tumorigenicity

Kobayashi, Yoshiomi, Okada, Yohei, Itakura, Go, Iwai, Hiroki, Nishimura, Soraya, Yasuda, Akimasa, Nori, Satoshi, Hikishima, Keigo, Konomi, Tsunehiko, Fujiyoshi, Kanehiro, Tsuji, Osahiko, Toyama, Yoshiaki, Yamanaka, Shinya, Nakamura, Masaya, Okano, Hideyuki

PLOS ONE 7 ( 12 ) 58 - 69 2012.12

Research paper (scientific journal),  ISSN  1932-6203

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Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc

Sakai, Daisuke, Nakamura, Yoshihiko, Nakai, Tomoko, Mishima, Taishi, Kato, Shunichi, Grad, Sibylle, Alini, Mauro, Risbud, Makarand V., Chan, Danny, Cheah, Kathryn S. E., Yamamura, Ken-ichi, Masuda, Koichi, Okano, Hideyuki, Ando, Kiyoshi, Mochida, Joji

NATURE COMMUNICATIONS 3   1067 - 1080 2012.12

Research paper (scientific journal),  ISSN  2041-1723

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MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice

Takano, Morito, Hikishima, Keigo, Fujiyoshi, Kanehiro, Shibata, Shinsuke, Yasuda, Akimasa, Konomi, Tsunehiko, Hayashi, Akiko, Baba, Hiroko, Honke, Koichi, Toyama, Yoshiaki, Okano, Hideyuki, Nakamura, Masaya

PLOS ONE 7 ( 12 ) 6106 - 6125 2012.12

Research paper (scientific journal),  ISSN  1932-6203

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Efficient Derivation of Multipotent Neural Stem/Progenitor Cells from Non-Human Primate Embryonic Stem Cells

Shimada, Hiroko, Okada, Yohei, Ibata, Keiji, Ebise, Hayao, Ota, Shin-ichi, Tomioka, Ikuo, Nomura, Toshihiro, Maeda, Takuji, Kohda, Kazuhisa, Yuzaki, Michisuke, Sasaki, Erika, Nakamura, Masaya, Okano, Hideyuki

PLOS ONE 7 ( 11 ) 24561 - 24570 2012.11

Research paper (scientific journal),  ISSN  1932-6203

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The Semaphorin 3A Inhibitor SM-345431 Accelerates Peripheral Nerve Regeneration and Sensitivity in a Murine Corneal Transplantation Model

Omoto, Masahiro, Yoshida, Satoru, Miyashita, Hideyuki, Kawakita, Tetsuya, Yoshida, Kenji, Kishino, Akiyoshi, Kimura, Toru, Shibata, Shinsuke, Tsubota, Kazuo, Okano, Hideyuki, Shimmura, Shigeto

PLOS ONE 7 ( 11 ) 106 - 114 2012.11

Research paper (scientific journal),  ISSN  1932-6203

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Sustained bFGF-Release Tubes for Peripheral Nerve Regeneration: Comparison with Autograft

Takagi, Takehiko, Kimura, Yu, Shibata, Shinsuke, Saito, Harukazu, Ishii, Ken, Okano, Hirotaka J., Toyama, Yoshiaki, Okano, Hideyuki, Tabata, Yasuhiko, Nakamura, Masaya

PLASTIC AND RECONSTRUCTIVE SURGERY 130 ( 4 ) 866 - 876 2012.10

Research paper (scientific journal),  ISSN  0032-1052

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Identification of mutations in the prostaglandin transporter gene SLCO2A1 and its phenotype-genotype correlation in Japanese patients with pachydermoperiostosis

Sasaki, Takashi, Niizeki, Hironori, Shimizu, Atsushi, Shiohama, Aiko, Hirakiyama, Asami, Okuyama, Torayuki, Seki, Atsuhito, Kabashima, Kenji, Otsuka, Atsushi, Ishiko, Akira, Tanese, Keiji, Miyakawa, Shun-ichi, Sakabe, Jun-ichi, Kuwahara, Masamitsu, Amagai, Masayuki, Okano, Hideyuki, Suematsu, Makoto, Kudoh, Jun

JOURNAL OF DERMATOLOGICAL SCIENCE 68 ( 1 ) 36 - 44 2012.10

Research paper (scientific journal),  ISSN  0923-1811

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