Papers - Okano, Hideyuki

Regeneration of the damaged CNS using human iPSCs-derived neural progenitor cells

Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E26 - E26 2011

Research paper (scientific journal),  ISSN  0168-0102

• Access to Document (DOI)

Molecular basis of the integrity of blood-brain barrier in Drosophila

Kanda, Hiroshi, Shimamura, Rieko, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E59 - E59 2011

Research paper (scientific journal),  ISSN  0168-0102

• Access to Document (DOI)

Eiger and Wengen: The Drosophila Orthologs of TNF/TNFR

Igaki, Tatsushi, Kanda, Hiroshi, Okano, Hideyuki, Xu, Tian, Miura, Masayuki

ADVANCES IN TNF FAMILY RESEARCH 691   45 - 50 2011

Research paper (scientific journal),  ISSN  0065-2598

• Access to Document (DOI)

Generation of neural crest progenitor cells from human induced pluripotent stem cells by a simple method

Ohta, Shigeki, Misawa, Aya, Okuno, Hironobu, Fukuda, Kimiko, Akamatsu, Wado, Kawakami, Yutaka, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E127 - E127 2011

Research paper (scientific journal),  ISSN  0168-0102

• Access to Document (DOI)

Efficient generation and developmental analysis of basal forebrain cholinergic neurons from mouse embryonic stem cells

Ishii, Seiji, Okada, Yohei, Miya, Fuyuki, Tsunoda, Tatsuhiko, Shimazaki, Takuya, Okano, Hideyuki

NEUROSCIENCE RESEARCH 71   E332 - E332 2011

Research paper (scientific journal),  ISSN  0168-0102

• Access to Document (DOI)

Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α

Houlihan Diarmaid D., Mabuchi Yo, Morikawa Satoru, Niibe Kunimichi, Araki Daisuke, Suzuki Sadafumi, Okano Hideyuki, Matsuzaki Yumi

Nature Protocols 7 ( 12 ) 2103 - 2111 2012.12

ISSN  1754-2189

　View Summary

<p>Platelet-derived growth factor receptor α (PDGFR-α) and stem cell antigen 1 (Sca-1) have recently been identified as selective markers of mouse mesenchymal stem cells (MSCs). PDGFR-α + Sca-1 + (PαS) MSCs have augmented growth potential and robust tri-lineage differentiation compared with standard culture-selected MSCs. In addition, the selective isolation of PαS MSCs avoids cellular contamination that can complicate other methods. Here we describe in detail our protocol to isolate PαS MSCs using flow cytometry. In brief, the tibia and femora are isolated and crushed using a pestle and mortar. The crushed bones are then chopped and incubated for 1 h at 37°C in 20 ml of DMEM containing 0.2% (wt/vol) collagenase. The cell suspension is filtered before red blood cell lysis and incubated with the following antibodies: allophycocyanin (APC)-conjugated PDGFR-α, FITC-conjugated Sca-1, phycoerythrin (PE)-conjugated CD45 and Ter119. Appropriate gates are constructed on a cell sorter to exclude dead cells and lineage (CD45 + Ter-119 +)-positive cells. Approximately 10,000 PαS MSCs may then be isolated per mouse. The total protocol takes ∼7 h to complete. © 2012 Nature America, Inc.</p>

• Access to Document (DOI)

Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-alpha

Houlihan, Diarmaid D., Mabuchi, Yo, Morikawa, Satoru, Niibe, Kunimichi, Araki, Daisuke, Suzuki, Sadafumi, Okano, Hideyuki, Matsuzaki, Yumi

NATURE PROTOCOLS 7 ( 12 ) 2103 - 2111 2012.12

Research paper (scientific journal),  ISSN  1754-2189

• Access to Document (DOI)

The common marmoset as a novel animal model system for biomedical and neuroscience research applications

Okano, Hideyuki, Hikishima, Keigo, Iriki, Atsushi, Sasaki, Erika

SEMINARS IN FETAL &amp; NEONATAL MEDICINE 17 ( 6 ) 336 - 340 2012.12

Research paper (scientific journal),  ISSN  1744-165X

• Access to Document (DOI)

Balanced ubiquitination determines cellular responsiveness to extracellular stimuli

Mukai, Akiko, Yamamoto-Hino, Miki, Komada, Masayuki, Okano, Hideyuki, Goto, Satoshi

CELLULAR AND MOLECULAR LIFE SCIENCES 69 ( 23 ) 4007 - 4016 2012.12

Research paper (scientific journal),  ISSN  1420-682X

• Access to Document (DOI)

Conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography: Preclinical longitudinal study in common marmosets

Konomi, Tsunehiko, Fujiyoshi, Kanehiro, Hikishima, Keigo, Komaki, Yuji, Tsuji, Osahiko, Okano, Hirotaka James, Toyama, Yoshiaki, Okano, Hideyuki, Nakamura, Masaya

NEUROIMAGE 63 ( 4 ) 1841 - 1853 2012.12

Research paper (scientific journal),  ISSN  1053-8119

• Access to Document (DOI)

Comparative gene expression in developing cognitive circuits in common marmoset brain

Mashiko, Hiromi, Yoshida, Aya C., Kikuchi, Satomi S., Niimi, Kimie, Takahashi, Eiki, Aruga, Jun, Okano, Hideyuki, Shimogori, Tomomi

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE 30 ( 8 ) 669 - 669 2012.12

Research paper (scientific journal),  ISSN  0736-5748

• Access to Document (DOI)

Novel In Vivo Imaging Analysis of an Inner Ear Drug Delivery System in Mice: Comparison of Inner Ear Drug Concentrations over Time after Transtympanic and Systemic Injections

Kanzaki, Sho, Fujioka, Masato, Yasuda, Akimasa, Shibata, Shinsuke, Nakamura, Masaya, Okano, Hirotaka James, Ogawa, Kaoru, Okano, Hideyuki

PLOS ONE 7 ( 12 ) 163 - 175 2012.12

Research paper (scientific journal),  ISSN  1932-6203

• Access to Document (DOI)

Comparative Anatomy of Marmoset and Mouse Cortex from Genomic Expression (April, pg 5039, 2012)

Mashiko, Hiromi, Yoshida, Aya C., Kikuchi, Satomi S., Niimi, Kimie, Takahashi, Eiki, Aruga, Jun, Okano, Hideyuki, Shimogori, Tomomi

JOURNAL OF NEUROSCIENCE 32 ( 49 ) 17932 - 17932 2012.12

Research paper (scientific journal),  ISSN  0270-6474

• Access to Document (DOI)

Pre-Evaluated Safe Human iPSC-Derived Neural Stem Cells Promote Functional Recovery after Spinal Cord Injury in Common Marmoset without Tumorigenicity

Kobayashi, Yoshiomi, Okada, Yohei, Itakura, Go, Iwai, Hiroki, Nishimura, Soraya, Yasuda, Akimasa, Nori, Satoshi, Hikishima, Keigo, Konomi, Tsunehiko, Fujiyoshi, Kanehiro, Tsuji, Osahiko, Toyama, Yoshiaki, Yamanaka, Shinya, Nakamura, Masaya, Okano, Hideyuki

PLOS ONE 7 ( 12 ) 58 - 69 2012.12

Research paper (scientific journal),  ISSN  1932-6203

• Access to Document (DOI)

Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc

Sakai, Daisuke, Nakamura, Yoshihiko, Nakai, Tomoko, Mishima, Taishi, Kato, Shunichi, Grad, Sibylle, Alini, Mauro, Risbud, Makarand V., Chan, Danny, Cheah, Kathryn S. E., Yamamura, Ken-ichi, Masuda, Koichi, Okano, Hideyuki, Ando, Kiyoshi, Mochida, Joji

NATURE COMMUNICATIONS 3   1067 - 1080 2012.12

Research paper (scientific journal),  ISSN  2041-1723

• Access to Document (DOI)

MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice

Takano, Morito, Hikishima, Keigo, Fujiyoshi, Kanehiro, Shibata, Shinsuke, Yasuda, Akimasa, Konomi, Tsunehiko, Hayashi, Akiko, Baba, Hiroko, Honke, Koichi, Toyama, Yoshiaki, Okano, Hideyuki, Nakamura, Masaya

PLOS ONE 7 ( 12 ) 6106 - 6125 2012.12

Research paper (scientific journal),  ISSN  1932-6203

• Access to Document (DOI)

Efficient Derivation of Multipotent Neural Stem/Progenitor Cells from Non-Human Primate Embryonic Stem Cells

Shimada, Hiroko, Okada, Yohei, Ibata, Keiji, Ebise, Hayao, Ota, Shin-ichi, Tomioka, Ikuo, Nomura, Toshihiro, Maeda, Takuji, Kohda, Kazuhisa, Yuzaki, Michisuke, Sasaki, Erika, Nakamura, Masaya, Okano, Hideyuki

PLOS ONE 7 ( 11 ) 24561 - 24570 2012.11

Research paper (scientific journal),  ISSN  1932-6203

• Access to Document (DOI)

The Semaphorin 3A Inhibitor SM-345431 Accelerates Peripheral Nerve Regeneration and Sensitivity in a Murine Corneal Transplantation Model

Omoto, Masahiro, Yoshida, Satoru, Miyashita, Hideyuki, Kawakita, Tetsuya, Yoshida, Kenji, Kishino, Akiyoshi, Kimura, Toru, Shibata, Shinsuke, Tsubota, Kazuo, Okano, Hideyuki, Shimmura, Shigeto

PLOS ONE 7 ( 11 ) 106 - 114 2012.11

Research paper (scientific journal),  ISSN  1932-6203

• Access to Document (DOI)

Sustained bFGF-Release Tubes for Peripheral Nerve Regeneration: Comparison with Autograft

Takagi, Takehiko, Kimura, Yu, Shibata, Shinsuke, Saito, Harukazu, Ishii, Ken, Okano, Hirotaka J., Toyama, Yoshiaki, Okano, Hideyuki, Tabata, Yasuhiko, Nakamura, Masaya

PLASTIC AND RECONSTRUCTIVE SURGERY 130 ( 4 ) 866 - 876 2012.10

Research paper (scientific journal),  ISSN  0032-1052

• Access to Document (DOI)

Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells

Kumamaru, Hiromi, Ohkawa, Yasuyuki, Saiwai, Hirokazu, Yamada, Hisakata, Kubota, Kensuke, Kobayakawa, Kazu, Akashi, Koichi, Okano, Hideyuki, Iwamoto, Yukihide, Okada, Seiji

NATURE COMMUNICATIONS 3   2811 - 2815 2012.10

Research paper (scientific journal),  ISSN  2041-1723

• Access to Document (DOI)