Papers - Okano, Hideyuki

Possibility of a novel therapy for neuropathic pain by specific histone modification of astrocytic chemokines in the spinal cord

Ikegami, Daigo, Ohi, Kaori, Narita, Michiko, Yamashita, Akira, Kuzumaki, Naoko, Okano, Hideyuki, Ushijima, Toshikazu, Narita, Minoru

JOURNAL OF PHARMACOLOGICAL SCIENCES 124   100P - 100P 2014

Research paper (scientific journal),  ISSN  1347-8613

Neural differentiation potentials in human embryonic stem cell lines

Fukumoto, Daigo, Matsumoto, Takuya, Okada, Yohei, Suemori, Hirofumi, Nakatsuji, Norio, Suzuki, Takeshi, Akamatsu, Wado, Okano, Hideyuki

JOURNAL OF PHARMACOLOGICAL SCIENCES 124   94P - 94P 2014

Research paper (scientific journal),  ISSN  1347-8613

Chronic itch requires STAT3-dependent reactive astrocytes in the spinal dorsal horn

Shiratori, Miho, Koga, Keisuke, Hasegawa, Ayumi, Toyonaga, Honami, Okano, Hideyuki, Hachisuka, Jun, Inoue, Kazuhide, Tsuda, Makoto

JOURNAL OF PHARMACOLOGICAL SCIENCES 124   81P - 81P 2014

Research paper (scientific journal),  ISSN  1347-8613

Analysis of glia-neuron interaction by using opto-fMRI

Tanaka, Kenji, Yoshida, Keitaro, Xu, Ming, Hikishima, Keigo, Okano, Hideyuki, Mimura, Masaru, Takata, Norio

JOURNAL OF PHARMACOLOGICAL SCIENCES 124   43P - 43P 2014

Research paper (scientific journal),  ISSN  1347-8613

I2020T mutant LRRK2 iPSC-derived neurons in the Sagamihara family exhibit increased Tau phosphorylation through the AKT/GSK-3 beta signaling pathway

Ohta, Etsuro, Nihira, Tomoko, Uchino, Akiko, Imaizumi, Yoichi, Okada, Yohei, Akamatsu, Wado, Takahashi, Kayoko, Hayakawa, Hideki, Nagai, Makiko, Ohyama, Manabu, Ryo, Masafuchi, Ogino, Mieko, Murayama, Shigeo, Takashima, Akihiko, Nishiyama, Kazutoshi, Mizuno, Yoshikuni, Mochizuki, Hideki, Obata, Fumiya, Okano, Hideyuki

HUMAN MOLECULAR GENETICS 24 ( 17 ) 4879 - 4900 2015.09

Research paper (scientific journal),  ISSN  0964-6906

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Histological and electrophysiological analysis of the corticospinal pathway to forelimb motoneurons in common marmosets

Kondo, Takahiro, Yoshihara, Yamato, Yoshino-Saito, Kimika, Sekiguchi, Tornofumi, Kosugi, Akito, Miyazaki, Yuta, Nishimura, Yukio, Okano, Hirotaka J., Nakamura, Masaya, Okano, Hideyuki, Isa, Tadashi, Ushiba, Junichi

NEUROSCIENCE RESEARCH 98   35 - 44 2015.09

Research paper (scientific journal),  ISSN  0168-0102

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MicroRNA-153 Regulates the Acquisition of Gliogenic Competence by Neural Stem Cells

Tsuyama, Jun, Bunt, Jens, Richards, Linda J., Iwanari, Hiroko, Mochizuki, Yasuhiro, Hamakubo, Takao, Shimazaki, Takuya, Okano, Hideyuki

STEM CELL REPORTS 5 ( 3 ) 365 - 377 2015.09

Research paper (scientific journal),  ISSN  2213-6711

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A new minimally-invasive method for microinjection into the mouse spinal dorsal horn

Kohro, Yuta, Sakaguchi, Emi, Tashima, Ryoichi, Tozaki-Saitoh, Hidetoshi, Okano, Hideyuki, Inoue, Kazuhide, Tsuda, Makoto

SCIENTIFIC REPORTS 5   101P - 101P 2015.09

Research paper (scientific journal),  ISSN  2045-2322

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Acute reduction of neuronal RNA binding Elavl2 protein and Gap43 mRNA in mouse hippocampus after kainic acid treatment

Ohtsuka Takafumi, Yano Masato, Okano Hideyuki

Biochemical and Biophysical Research Communications 466 ( 1 ) 46 - 51 2015.08

ISSN  0006-291X

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<p>Activity-dependent gene regulation in neurons has been hypothesized to be under transcriptional control and to include dramatic increases in immediate early genes (IEGs) after neuronal activity. In addition, several reports have focused on post-transcriptional regulation, which could be mediated by neuronal post-transcriptional regulators, including RNA binding proteins (RNABPs). One such protein family is the neuronal Elavls (nElavls; Elavl2, Elavl3, and Elavl4), whose members are widely expressed in peripheral and central nervous system. Previous reports showed that Elavl3 and 4 are up-regulated following repeated stimulation such as during cocaine administration, a seizure, or a spatial discrimination task. In this study, we focused on Elavl2, a candidate gene for schizophrenia and studied its role in neuronal activity. First we found that Elavl2 has a cell-type specific expression pattern that is highly expressed in hippocampal CA3 pyramidal neurons and hilar interneurons using Elavl2 specific antibody. Second, unexpectedly, we discovered that the Elavl2 protein level in the hippocampus was acutely down-regulated for 3 h after a kainic acid (KA)-induced seizure in the hippocampal CA3 region. In addition, level of Gap43 mRNA, a target mRNA of Elavl2 is decreased 12 h after KA treatment, thus suggesting the involvement of Elavl2 in activity-dependent RNA regulation.</p>

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Voxel-based morphometry of the marmoset brain

Hikishima K., Ando K., Komaki Y., Kawai K., Yano R., Inoue T., Itoh T., Yamada M., Momoshima S., Okano H. J., Okano H.

Neuroscience 300   585 - 592 2015.08

ISSN  0306-4522

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<p>Movement dysfunction in Parkinson's disease (PD) is caused by the degeneration of dopaminergic (DA) neurons in the substantia nigra (SN). Here, we established a method for voxel-based morphometry (VBM) and automatic tissue segmentation of the marmoset monkey brain using a 7-T animal scanner and applied the method to assess DA degeneration in a PD model, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated animals, with tyrosine-hydroxylase staining. The most significant decreases of local tissue volume were detected in the bilateral SN of MPTP-treated marmoset brains (-53.0% in right and -46.5% in left) and corresponded with the location of DA neurodegeneration found in histology (-65.4% in right). In addition to the SN, the decreases were also confirmed in the locus coeruleus, and lateral hypothalamus. VBM using 7-T MRI was effective in detecting volume loss in the SN of the PD-model marmoset. This study provides a potential basis for the application of VBM with ultra-high field MRI in the clinical diagnosis of PD. The developed method may also offer value in automatic whole-brain evaluation of structural changes for the marmoset monkey.</p>

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q-space MR imaging of gastric carcinoma ex vivo

Yamada Ichiro, Hikishima Keigo, Miyasaka Naoyuki, Kato Keiji, Ito Eisaku, Kojima Kazuyuki, Kawano Tatsuyuki, Kobayashi Daisuke, Eishi Yoshinobu, Okano Hideyuki

Magnetic Resonance in Medicine 76 ( 2 ) 602 - 612 2015.08

Research paper (scientific journal), Joint Work,  ISSN  0740-3194

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<p>Purpose: The purpose of this study was to establish the feasibility of q-space imaging (QSI) as a method of assessing the depth of mural invasion, histologic grade, and the presence of lymph node metastasis in gastric carcinomas. Methods: A 7.0 Tesla MR imaging system was used to investigate 20 gastric specimens containing a carcinoma. QSI was performed by using the following parameters: 50-60 mm × 25-30 mm field of view, 2-mm section thickness, 256 × 128 matrix, 10 b values in the 0-7163 s/mm&lt;sup&gt;2&lt;/sup&gt; range, which corresponded to q values of 0-1026/cm, and motion-probing gradients perpendicular to the gastric wall. The MR images and the histopathologic findings were then compared. Results: The depth of tumor invasion of the gastric wall in all 20 carcinomas (100%) was established by using mean displacement, zero-displacement probability, and kurtosis maps. The QSI parameters were significantly correlated with the histologic grades of the gastric carcinomas (all P&lt;0.001). The QSI parameters made it possible to differentiate between metastatic and nonmetastatic lymph nodes (all P=0.001). Conclusion: Ex vivo QSI facilitates excellent diagnostics for evaluating gastric carcinomas in terms of mural invasion, histologic grade, and the presence of lymph node metastasis.</p>

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Migration and differentiation of transplanted enteric neural crest-derived cells in murine model of Hirschsprung's disease

Nishikawa, Ryuhei, Hotta, Ryo, Shimojima, Naoki, Shibata, Shinsuke, Nagoshi, Narihito, Nakamura, Masaya, Matsuzaki, Yumi, Okano, Hirotaka J., Kuroda, Tatsuo, Okano, Hideyuki, Morikawa, Yasuhide

CYTOTECHNOLOGY 67 ( 4 ) 661 - 670 2015.08

Research paper (scientific journal),  ISSN  0920-9069

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CD34 and CD49f Double-Positive and Lineage Marker-Negative Cells Isolated from Human Myometrium Exhibit Stem Cell-Like Properties Involved in Pregnancy-Induced Uterine Remodeling

Ono, Masanori, Kajitani, Takashi, Uchida, Hiroshi, Arase, Toru, Oda, Hideyuki, Uchida, Sayaka, Ota, Kuniaki, Nagashima, Takashi, Masuda, Hirotaka, Miyazaki, Kaoru, Asada, Hironori, Hida, Naoko, Mabuchi, Yo, Morikawa, Satoru, Ito, Mamoru, Bulun, Serdar E., Okano, Hideyuki, Matsuzaki, Yumi, Yoshimura, Yasunori, Maruyama, Tetsuo

BIOLOGY OF REPRODUCTION 93 ( 2 ) 17163 - 17168 2015.08

Research paper (scientific journal),  ISSN  0006-3363

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Engrafted Neural Stem/Progenitor Cells Promote Functional Recovery through Synapse Reorganization with Spared Host Neurons after Spinal Cord Injury

Yokota, Kazuya, Kobayakawa, Kazu, Kubota, Kensuke, Miyawaki, Atsushi, Okano, Hideyuki, Ohkawa, Yasuyuki, Iwamoto, Yukihide, Okada, Seiji

STEM CELL REPORTS 5 ( 2 ) 264 - 277 2015.08

Research paper (scientific journal),  ISSN  2213-6711

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BDNF Induced by Treadmill Training Contributes to the Suppression of Spasticity and Allodynia After Spinal Cord Injury via Upregulation of KCC2

Tashiro, Syoichi, Shinozaki, Munehisa, Mukaino, Masahiko, Renault-Mihara, Francois, Toyama, Yoshiaki, Liu, Meigen, Nakamura, Masaya, Okano, Hideyuki

NEUROREHABILITATION AND NEURAL REPAIR 29 ( 7 ) 677 - 689 2015.08

Research paper (scientific journal),  ISSN  1545-9683

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Neural stem/progenitor cell-laden microfibers promote transplant survival in a mouse transected spinal cord injury model

Sugai Keiko, Nishimura Soraya, Kato-Negishi Midori, Onoe Hiroaki, Iwanaga Shintaroh, Toyama Yoshiaki, Matsumoto Morio, Takeuchi Shoji, Okano Hideyuki, Nakamura Masaya

Journal of Neuroscience Research 93 ( 12 ) 1826 - 1838 2015.08

Joint Work,  ISSN  0360-4012

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<p>Previous studies have demonstrated that transplantation of neural stem/progenitor cells (NS/PCs) into the lesioned spinal cord can promote functional recovery following incomplete spinal cord injury (SCI) in animal models. However, this strategy is insufficient following complete SCI because of the gap at the lesion epicenter. To obtain functional recovery in a mouse model of complete SCI, this study uses a novel collagen-based microfiber as a scaffold for engrafted NS/PCs. We hypothesized that the NS/PC-microfiber combination would facilitate lesion closure as well as transplant survival in the transected spinal cord. NS/PCs were seeded inside the novel microfibers, where they maintained their capacity to differentiate and proliferate. After transplantation, the stumps of the transected spinal cord were successfully bridged by the NS/PC-laden microfibers. Moreover, the transplanted cells migrated into the host spinal cord and differentiated into three neural lineages (astrocytes, neurons, and oligodendrocytes). However, the NS/PC-laden scaffold could not achieve a neural connection between the rostral end of the injury and the intact caudal area of the spinal cord, nor could it achieve recovery of motor function. To obtain optimal functional recovery, a microfiber design with a modified composition may be useful. Furthermore, combinatorial therapy with rehabilitation and/or medications should also be considered for practical success of biomaterial/cell transplantation-based approaches to regenerative medicine.</p>

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Utility of Scalp Hair Follicles as a Novel Source of Biomarker Genes for Psychiatric Illnesses

Maekawa, Motoko, Yamada, Kazuo, Toyoshima, Manabu, Ohnishi, Tetsuo, Iwayama, Yoshimi, Shimamoto, Chie, Toyota, Tomoko, Nozaki, Yayoi, Balan, Shabeesh, Matsuzaki, Hideo, Iwata, Yasuhide, Suzuki, Katsuaki, Miyashita, Mitsuhiro, Kikuchi, Mitsuru, Kato, Motoichiro, Okada, Yohei, Akamatsu, Wado, Mori, Norio, Owada, Yuji, Itokawa, Masanari, Okano, Hideyuki, Yoshikawa, Takeo

BIOLOGICAL PSYCHIATRY 78 ( 2 ) 116 - 125 2015.07

Research paper (scientific journal),  ISSN  0006-3223

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Allogeneic Neural Stem/Progenitor Cells Derived From Embryonic Stem Cells Promote Functional Recovery After Transplantation Into Injured Spinal Cord of Nonhuman Primates

Iwai, Hiroki, Shimada, Hiroko, Nishimura, Soraya, Kobayashi, Yoshiomi, Itakura, Go, Hori, Keiko, Hikishima, Keigo, Ebise, Hayao, Negishi, Naoko, Shibata, Shinsuke, Habu, Sonoko, Toyama, Yoshiaki, Nakamura, Masaya, Okano, Hideyuki

STEM CELLS TRANSLATIONAL MEDICINE 4 ( 7 ) 708 - 719 2015.07

Research paper (scientific journal),  ISSN  2157-6564

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Esophageal carcinoma: Evaluation with q-space diffusion-weighted MR imaging ex vivo

Yamada, Ichiro, Hikishima, Keigo, Miyasaka, Naoyuki, Tokairin, Yutaka, Ito, Eisaku, Kawano, Tatsuyuki, Kobayashi, Daisuke, Eishi, Yoshinobu, Okano, Hideyuki

MAGNETIC RESONANCE IN MEDICINE 73 ( 6 ) 2262 - 2273 2015.06

Research paper (scientific journal),  ISSN  0740-3194

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Gastric Carcinoma: Ex Vivo MR Imaging at 7.0 T-Correlation with Histopathologic Findings

Yamada, Ichiro, Miyasaka, Naoyuki, Hikishima, Keigo, Kato, Keiji, Kojima, Kazuyuki, Kawano, Tatsuyuki, Ito, Eisaku, Kobayashi, Daisuke, Eishi, Yoshinobu, Okano, Hideyuki

RADIOLOGY 275 ( 3 ) 841 - 848 2015.06

Research paper (scientific journal),  ISSN  0033-8419

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