Details of a Researcher - Okano, Hideyuki

Papers - Okano, Hideyuki

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Structure of Musashi1 in a complex with target RNA: the role of aromatic stacking interactions

Ohyama, Takako, Nagata, Takashi, Tsuda, Kengo, Kobayashi, Naohiro, Imai, Takao, Okano, Hideyuki, Yamazaki, Toshio, Katahira, Masato

NUCLEIC ACIDS RESEARCH 40 ( 7 ) 3218 - 3231 2012.04

Research paper (scientific journal), ISSN 0305-1048

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Comparative Anatomy of Marmoset and Mouse Cortex from Genomic Expression

Mashiko, Hiromi, Yoshida, Aya C., Kikuchi, Satomi S., Niimi, Kimie, Takahashi, Eiki, Aruga, Jun, Okano, Hideyuki, Shimogori, Tomomi

JOURNAL OF NEUROSCIENCE 32 ( 15 ) 5039 - 5053 2012.04

Research paper (scientific journal), ISSN 0270-6474

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Derivation of Human Differential Photoreceptor-like Cells from the Iris by Defined Combinations of CRX, RX and NEUROD

Seko, Yuko, Azuma, Noriyuki, Kaneda, Makoto, Nakatani, Kei, Miyagawa, Yoshitaka, Noshiro, Yuuki, Kurokawa, Reiko, Okano, Hideyuki, Umezawa, Akihiro

PLOS ONE 7 ( 4 ) S230 - S230 2012.04

Research paper (scientific journal), ISSN 1932-6203

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Effect of-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells

Kuzumaki N., Suzuki A., Narita M., Hosoya T., Nagasawa A., Imai S., Yamamizu K., Morita H., Nagase H., Okada Y., Okano H. J., Yamashita J. K., Okano H., Suzuki T., Narita M.

British Journal of Cancer 106 ( 6 ) 1148 - 1152 2012.03

ISSN 0007-0920

Background: It is becoming increasingly recognised that opioids are responsible for tumour growth. However, the effects of opioids on tumour growth have been controversial.Methods:The effects of-opioid receptor (KOR) agonist on the growth of non-small cell lung cancer (NSCLC) cells were assessed by a cell proliferation assay. Western blotting was performed to ascertain the mechanism by which treatment with KOR agonist suppresses tumour growth. Results: Addition of the selective KOR agonist U50,488H to gefitinib-sensitive (HCC827) and gefitinib-resistant (H1975) NSCLC cells produced a concentration-dependent decrease in their growth. These effects were abolished by co-treatment with the selective KOR antagonist nor-BNI. Furthermore, the growth-inhibitory effect of gefitinib in HCC827 cells was further enhanced by co-treatment with U50,488H. With regard to the inhibition of tumour growth, the addition of U50, 488H to H1975 cells produced a concentration-dependent decrease in phosphorylated- glycogen synthase kinase 3Β (p-GSK3Β).Conclusion:The present results showed that stimulation of KOR reduces the growth of gefitinib-resistant NSCLC cells through the activation of GSK3Β. © 2012 Cancer Research UK All rights reserved.

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The first clinical trial in Tohoku university hospital after the Great East JAPAN Earthquake

Okano Hideyuki

Keio Journal of Medicine 61 ( 1 ) 3 - 9 2012.03

ISSN 0022-9717

The Great East Japan Earthquake of 2011 seriously jeopardized our collaborative research with Professor Masashi Aoki (Tohoku University School of Medicine) on the development of new therapies for amyotrophic lateral sclerosis (ALS) using hepatocyte growth factor. After the earthquake struck, Professor Aoki made a tremendous contribution to saving patients' lives and to recovering from the disastrous situation. Thanks to his strong leadership and support from many reliable colleagues, we could finally start new clinical trials for ALS patients. In this article, I wish to introduce Professor Aoki's heroic efforts. © 2012 by The Keio Journal of Medicine.

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The formation of an angiogenic astrocyte template is regulated by the neuroretina in a HIF-1-dependent manner

Nakamura-Ishizu, Ayako, Kurihara, Toshihide, Okuno, Yuji, Ozawa, Yoko, Kishi, Kazuo, Goda, Nobuhito, Tsubota, Kazuo, Okano, Hideyuki, Suda, Toshio, Kubota, Yoshiaki

DEVELOPMENTAL BIOLOGY 363 ( 1 ) 106 - 114 2012.03

Research paper (scientific journal), ISSN 0012-1606

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RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI3 Kinase/Akt Signaling Pathways

Muto, Jun, Imai, Takao, Ogawa, Daisuke, Nishimoto, Yoshinori, Okada, Yohei, Mabuchi, Yo, Kawase, Takeshi, Iwanami, Akio, Mischel, Paul S., Saya, Hideyuki, Yoshida, Kazunari, Matsuzaki, Yumi, Okano, Hideyuki

PLOS ONE 7 ( 3 ) 1223 - 1237 2012.03

Research paper (scientific journal), ISSN 1932-6203

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RNA-Binding Protein HuD Controls Insulin Translation

Lee, Eun Kyung, Kim, Wook, Tominaga, Kumiko, Martindale, Jennifer L., Yang, Xiaoling, Subaran, Sarah S., Carlson, Olga D., Mercken, Evi M., Kulkarni, Rohit N., Akamatsu, Wado, Okano, Hideyuki, Perrone-Bizzozero, Nora I., de Cabo, Rafael, Egan, Josephine M., Gorospe, Myriam

MOLECULAR CELL 45 ( 6 ) 826 - 835 2012.03

Research paper (scientific journal), ISSN 1097-2765

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Identification of Proteasome Components Required for Apical Localization of Chaoptin Using Functional Genomics

Yano, Hiroyuki, Yamamoto-Hino, Miki, Awano, Wakae, Aoki-Kinoshita, Kiyoko F., Tsuda-Sakurai, Kayoko, Okano, Hideyuki, Goto, Satoshi

JOURNAL OF NEUROGENETICS 26 ( 1 ) 53 - 63 2012.03

Research paper (scientific journal), ISSN 0167-7063

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Bioluminescent system for dynamic imaging of cell and animal behavior

Hara-Miyauchi, Chikako, Tsuji, Osahiko, Hanyu, Aki, Okada, Seiji, Yasuda, Akimasa, Fukano, Takashi, Akazawa, Chihiro, Nakamura, Masaya, Imamura, Takeshi, Matsuzaki, Yumi, Okano, Hirotaka James, Miyawaki, Atsushi, Okano, Hideyuki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 419 ( 2 ) 188 - 193 2012.03

Research paper (scientific journal), ISSN 0006-291X

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Structure, function and formation of glycans in Drosophila

Yamamoto-Hino Miki, Okano Hideyuki, Kanie Osamu, Goto Satoshi

165 - 188 2012.02

Glycosylation of proteins shows two striking features: first, there are a large number of glycan structures that are involved in various biological events (diversity) and, second, certain structures are selectively added to specific sites within proteins (specificity) in a context-dependent manner. However, the mechanisms that regulate diversity and specificity are unknown. Over the last few decades, genetic studies in Drosophila have been used to examine the functions mediated by glycosylation in vivo. Recent improvements in the combined use of biochemical/biophysical, genetic, and cell biology approaches has allowed the rapid and detailed analysis of the structure, function and formation of glycans in Drosophila. This chapter aims to provide an overview of the different types of glycans and their biological function(s) in Drosophila. The regulation of glycan synthesis in Drosophila and mammals is also discussed. © 2012 by Nova Science Publishers, Inc. All rights reserved.

Abundant Occurrence of Basal Radial Glia in the Subventricular Zone of Embryonic Neocortex of a Lissencephalic Primate, the Common Marmoset Callithrix jacchus

Kelava, Iva, Reillo, Isabel, Murayama, Ayako Y., Kalinka, Alex T., Stenzel, Denise, Tomancak, Pavel, Matsuzaki, Fumio, Lebrand, Cecile, Sasaki, Erika, Schwamborn, Jens C., Okano, Hideyuki, Huttner, Wieland B., Borrell, Victor

CEREBRAL CORTEX 22 ( 2 ) 469 - 481 2012.02

Research paper (scientific journal), ISSN 1047-3211

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Therapeutic potential of induced pluripotent stem cells for spinal cord injury

Nori Satoshi, Tsuji Osahiko, Okada Yohei, Toyama Yoshiaki, Okano Hideyuki, Nakamura Masaya

Brain and Nerve 64 ( 1 ) 17 - 27 2012.01

ISSN 1881-6096

Once the safety issue has been overcome, induced pluripotent stem cells (iPSCs), which do not entail ethical or immunological concerns, may become the preferred cell source for regenerative medicine. Various types of iPSCs have been established by different methods, and each type exhibits different biological properties. Before iPSC-based clinical applications can be initiated, detailed evaluations of the cells, including their differentiation potentials and tumorigenic activities in different contexts, should be investigated to establish their safety and effectiveness for cell transplantation therapies. Recently, we demonstrated the directed neural differentiation of mouse iPSCs and examined their therapeutic potential in a mouse spinal cord injury (SCI) model. Mouse iPSC-derived neural stem/progenitor cells (NS/PCs), which had been pre-evaluated as non-tumorigenic by their transplantation into nonobese diabetic-severe combined immunodeficiency (NOD-scid) mouse brain, were transplanted into the spinal cord 9 days after SCI. Mouse iPSC-derived NS/PCs differentiated into all three neural lineages without forming teratomas or other tumors. They also participated in re-myelination and induced the axonal re-growth, promoting motor functional recovery. Nevertheless, our results constitute only the first step toward clinical application. The safety and effectiveness of human iPSC-derived NS/PCs need to be more intensively investigated in future preclinical studies, for example, using non-human primate SCI models. In particular, human iPSCs established by delivering reprogramming factors using a safer method than retrovirus system, such as an integration-free virus system, virus-free system, or transgene-free system should be evaluated.

Derivation of human differential photoreceptor-like cells from the iris by defined combinations of CRX, RX and NEUROD.

Seko Yuko, Azuma Noriyuki, Kaneda Makoto, Nakatani Kei, Miyagawa Yoshitaka, Noshiro Yuuki, Kurokawa Reiko, Okano Hideyuki, Umezawa Akihiro

PLoS ONE 7 ( 4 ) 2012

Examples of direct differentiation by defined transcription factors have been provided for beta-cells, cardiomyocytes and neurons. In the human visual system, there are four kinds of photoreceptors in the retina. Neural retina and iris-pigmented epithelium (IPE) share a common developmental origin, leading us to test whether human iris cells could differentiate to retinal neurons. We here define the transcription factor combinations that can determine human photoreceptor cell fate. Expression of rhodopsin, blue opsin and green/red opsin in induced photoreceptor cells were dependent on combinations of transcription factors: A combination of CRX and NEUROD induced rhodopsin and blue opsin, but did not induce green opsin; a combination of CRX and RX induced blue opsin and green/red opsin, but did not induce rhodopsin. Phototransduction-related genes as well as opsin genes were up-regulated in those cells. Functional analysis; i.e. patch clamp recordings, clearly revealed that generated photoreceptor cells, induced by CRX, RX and NEUROD, responded to light. The response was an inward current instead of the typical outward current. These data suggest that photosensitive photoreceptor cells can be generated by combinations of transcription factors. The combination of CRX and RX generate immature photoreceptors: and additional NEUROD promotes maturation. These findings contribute substantially to a major advance toward eventual cell-based therapy for retinal degenerative diseases.

Regeneration of the damaged CNS using human iPSCs-derived neural progenitor cells

Okano, Hideyuki

JOURNAL OF PHARMACOLOGICAL SCIENCES 118 48P - 48P 2012

Research paper (scientific journal), ISSN 1347-8613

MicroRNAs in neural stem cells and neurogenesis

Kawahara, Hironori, Imai, Takao, Okano, Hideyuki

FRONTIERS IN NEUROSCIENCE 6 S347 - S348 2012

Research paper (scientific journal), ISSN 1662-453X

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NOTCH SIGNALING AND THE DEVELOPING INNER EAR

Murata, Junko, Ikeda, Katsuhisa, Okano, Hideyuki

NOTCH SIGNALING IN EMBRYOLOGY AND CANCER 727 161 - 173 2012

Research paper (scientific journal), ISSN 0065-2598

Usefulness of human disease-specific iPS cells research for understanding of age-related higher brain dysfunction

Kuzumaki, Naoko, Narita, Michiko, Suda, Yukari, Nagasawa, Atsumi, Hamada, Yusuke, Akamatsu, Wado, Okada, Yohei, James, Hirotaka Okano, Okano, Hideyuki, Narita, Minoru

JOURNAL OF PHARMACOLOGICAL SCIENCES 118 124P - 124P 2012

Research paper (scientific journal), ISSN 1347-8613

Nestin Protein Is Phosphorylated in Adult Neural Stem/Progenitor Cells and Not Endothelial Progenitor Cells

Namiki, Jun, Suzuki, Sayuri, Masuda, Takeshi, Ishihama, Yasushi, Okano, Hideyuki

STEM CELLS INTERNATIONAL 1410 - 1422 2012

Research paper (scientific journal), ISSN 1687-966X

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Renin-angiotensin system hyperactivation can induce inflammation and retinal neural dysfunction

Kurihara Toshihide, Ozawa Yoko, Ishida Susumu, Okano Hideyuki, Tsubota Kazuo

International Journal of Inflammation 2012 2012

ISSN 2090-8040

The renin-angiotensin system (RAS) is a hormone system that has been classically known as a blood pressure regulator but is becoming well recognized as a proinflammatory mediator. In many diverse tissues, RAS pathway elements are also produced intrinsically, making it possible for tissues to respond more dynamically to systemic or local cues. While RAS is important for controlling normal inflammatory responses, hyperactivation of the pathway can cause neural dysfunction by inducing accelerated degradation of some neuronal proteins such as synaptophysin and by activating pathological glial responses. Chronic inflammation and oxidative stress are risk factors for high incidence vision-threatening diseases such as diabetic retinopathy (DR), age-related macular degeneration (AMD), and glaucoma. In fact, increasing evidence suggests that RAS inhibition may actually prevent progression of various ocular diseases including uveitis, DR, AMD, and glaucoma. Therefore, RAS inhibition may be a promising therapeutic approach to fine-tune inflammatory responses and to prevent or treat certain ocular and neurodegenerative diseases. © 2012 Toshihide Kurihara et al.

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