Papers - Okano, Hideyuki

SOCS3 is required to protect photoreceptor cell function during retinal inflammation

Ozawa, Yoko, Nakao, Keiko, Kurihara, Trishilhide, Shimazaki, Takuya, Shimmura, Shigeto, Ishida, Susumu, Yoshimura, Akihiko, Tsubota, Kazuo, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S269 - S269 2008

Research paper (scientific journal),  ISSN  0168-0102

Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP

Kawahara, Hironori, Imai, Takao, Imataka, Hiroaki, Tujimoto, Masafumi, Matsumoto, Ken, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S153 - S153 2008

Research paper (scientific journal),  ISSN  0168-0102

Anti IL-6 antibody treatment reduces the infiltration of hematogenous macrophages into the injured spinal cord

Mukaino, Masahiko, Nakamura, Masaya, Yamada, Osama, Okada, Seiji, Morikawa, Satoru, Osugi, Yoshiyuki, Matsuzaki, Yumi, Toyama, Yoshiaki, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S275 - S275 2008

Research paper (scientific journal),  ISSN  0168-0102

A role of neuronal RNA-binding protein Hu and NF45/90 in the nervous system

Igarashi, Mania, Yano, Masato, Okano, Hirotaka J., Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S153 - S153 2008

Research paper (scientific journal),  ISSN  0168-0102

Expression of free fatty acid receptor GPR40 in the neurogenic niche of adult monkey hippocampus

Ma, Dexuan, Lu, Li, Boneva, Nadezhda B., Warashina, Shogo, Kaplamadzhiev, Desislav B., Mori, Yoshimi, Nakaya, Masa-aki, Kikuchi, Mitsuru, Tonchev, Anton B., Okano, Hideyuki, Yamashima, Tetsurnori

HIPPOCAMPUS 18 ( 3 ) 326 - 333 2008

Research paper (scientific journal),  ISSN  1050-9631

• Access to Document (DOI)

Mature Adipocyte-Derived Cells, Dedifferentiated Fat Cells (DFAT), Promoted Functional Recovery From Spinal Cord Injury-Induced Motor Dysfunction in Rats

Ohta, Yuki, Takenaga, Mitsuko, Tokura, Yukie, Hamaguchi, Akemi, Matsumoto, Taro, Kano, Koichiro, Mugishima, Hideo, Okano, Hideyuki, Igarashi, Rie

CELL TRANSPLANTATION 17 ( 8 ) 877 - 886 2008

Research paper (scientific journal),  ISSN  0963-6897

In vivo imaging in humanized mice

Masuda H., Okano H. J., Maruyama T., Yoshimura Y., Okano H., Matsuzaki Y.

Current topics in microbiology and immunology 324   179 - 196 2008

ISSN  0070-217X

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<p>The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations. © 2008 Springer-Verlag.</p>

• Access to Document (DOI)

Structural analysis of Musashi-RNA complex on the basis of long-range structural information.

Ohyama Takako, Furukawa Ayako, Mashima Tsukasa, Sugiyama Takashi, Ohgara Shouta, Yamazaki Toshio, Imai Takao, Okano Hideyuki, Nagata Takashi, Katahira Masato

Nucleic acids symposium series (2004)  ( 52 ) 193 - 194 2008

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<p>Musashi protein is supposed to be involved in the regulation of differentiation of neural stem cells. Musashi binds to 3' untranslated region of target mRNA and represses the translation of mRNA. Musashi has two tandem RNA-binding domains (RBDs), RBD1 and RBD2. Both RBDs cooperatively bind to the target mRNA. Here, we determined the structure of RBD1-RBD2 in complex with target RNA. First, the structures of two RBDs in the complex were determined on the basis of short-range distance restrains derived from NOEs. However, the relative position of two RBDs was not determined due to the lack of long-range distance restraints across two RBDs. In order to overcome the situation, we introduced the paramagnetic center into Musashi by attaching MTSL carrying the NO radical. The long-range distance restraints (ca. 20-40 A) between two RBDs were derived from paramagnetic relaxation enhancement (PRE) caused by the paramagnetic center. The relative position of two RBDs was successfully determined on the basis of these long-range distance restraints. The change in the relative position of two RBDs on binding to the target RNA was also detected by PRE. The determined structure of RBD1-RBD2 in the complex has suggested how Musashi recognizes its target mRNA.</p>

Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats

Ohta Yuki, Takenaga Mitsuko, Tokura Yukie, Hamaguchi Akemi, Matsuraoto Taro, Kano Koichiro, Mugishima Hideo, Okano Hideyuki, Igarashi Rie

Cell Transplantation 17 ( 8 ) 877 - 886 2008

ISSN  0963-6897

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<p>Transplantation of mature adipocyte-derived cells (dedifferentiated fat cells) led to marked functional recovery from spinal cord injury (SCI)-induced motor dysfunction in rats. When mature adipocytes were isolated from rat adipose tissue and grown in ceiling culture, transformation into fibroblast-like cells without lipid droplets occurred. These fibroblast-like cells, termed dedifferentiated fat cells (DFAT), could proliferate and could also differentiate back into adipocytes. DFAT expressed neural markers such as nestin, βIII tubulin, and GFAP. Allografting of DFAT into SCI-induced rats led to significant recovery from hindlimb dysfunction. Grafted cells were detected at the injection site, and some of these cells expressed βIII tubulin. DFAT expressed neurotrophic factors such as BDNF and GDNF prior to transplantation, and grafted cells were also positive for these factors. Therefore, these neurotrophic factors derived from grafted DFAT might have contributed to the promotion of functional recovery. These findings also suggest that mature adipocytes could become a new source for cell replacement therapy to treat central nervous system disorders. Copyright © 2008 Cognizant Comm. Corp.</p>

Switching mechanism to neural differentiation controlled by Musashi and Hu proteins.

Kakumoto, Kyoko, Okano, Okano, Shibata, Shinsuke, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S155 - S155 2008

Research paper (scientific journal),  ISSN  0168-0102

Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP

Kawahara, Hironori, Imai, Takao, Imataka, Hiroaki, Tujimoto, Masafumi, Matsumoto, Ken, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S153 - S153 2008

Research paper (scientific journal),  ISSN  0168-0102

Expression of free fatty acid receptor GPR40 in the neurogenic niche of adult monkey hippocampus

Ma, Dexuan, Lu, Li, Boneva, Nadezhda B., Warashina, Shogo, Kaplamadzhiev, Desislav B., Mori, Yoshimi, Nakaya, Masa-aki, Kikuchi, Mitsuru, Tonchev, Anton B., Okano, Hideyuki, Yamashima, Tetsurnori

HIPPOCAMPUS 18 ( 3 ) 326 - 333 2008

Research paper (scientific journal),  ISSN  1050-9631

• Access to Document (DOI)

Analysis of RNA binding protein Musashi2 in mammalian nervous system development

Shibata, Shinsuke, Sakakibara, Shin-Ichi, Okano, Hirotaka J., Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S89 - S89 2008

Research paper (scientific journal),  ISSN  0168-0102

Stromal cell-secreted factors promote the survival of ES cell-derived early neural stem/progenitor cells via the activation of MAPK and PI3K-Akt pathways

Ishii, Seiji, Okada, Yohei, Shimazaki, Takuya, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S156 - S156 2008

Research paper (scientific journal),  ISSN  0168-0102

Neuroepithelial stem cells are converted into neural progenitors during an elongated interphase in Drosophila larval optic lobe

Orihara, Minako, Okano, Hideyuki, Nakao, Keiko

NEUROSCIENCE RESEARCH 61   S156 - S156 2008

Research paper (scientific journal),  ISSN  0168-0102

Mechanisms of reactive astrocytes migration after spina cord injury

Renault-Mihara, Francois, Mukaino, Masahiko, Yamane, Junichi, Nakamura, Masaya, Toyama, Yoshiaki, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S227 - S227 2008

Research paper (scientific journal),  ISSN  0168-0102

Intraarterial transplantation of mononuclear cells promotes neovascularization after transient cerebral ischemia in mice

Funabiki, Tomohiro, Namiki, Jun, Suzuki, Sayuri, Matsuzaki, Yumi, Aikawa, Naoki, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S134 - S134 2008

Research paper (scientific journal),  ISSN  0168-0102

An efficient screening method using inner-ear-derived spheres for selection of compounds that induce hair cell differentiation

Hosoya, Makoto, Fujioka, Masato, Jeon, Sang-Jun, Okano, Hirotaka J., Edge, Albert, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S57 - S57 2008

Research paper (scientific journal),  ISSN  0168-0102

Cell therapy for spinal cord injury by neural stem/progenitor cells derived from induced pluripotent stem cells

Tsuji, Osahiko, Miura, Kyoko, Nakamura, Masaya, Nagoshi, Narihito, Kitamura, Kazuya, Fujiyoshi, Kanehiro, Mukaino, Masahiko, Kumagai, Gentaro, Katoh, Hiroyuki, Yohei, Okada, Yamanaka, Shinya, Toyama, Yoshiaki, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S69 - S69 2008

Research paper (scientific journal),  ISSN  0168-0102

The long-term live monitoring of transplanted human fetal neural stem/progenitor cells in vivo

Ogawa, Daisuke, Okada, Yohei, Nakamura, Masaya, Okano, James Hirotaka, Kanemura, Yonehiro, Matsuzaki, Yumi, Shimazaki, Takuya, Ito, Mamoru, Ikeda, Eiji, Tamiya, Takashi, Nagao, Seigo, Okano, Hideyuki

NEUROSCIENCE RESEARCH 61   S85 - S85 2008

Research paper (scientific journal),  ISSN  0168-0102