論文 - 岡野　栄之

Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis

Kawashima Takeshi, Murakami Akikazu R., Ogasawara Michio, Tanaka Kimio J., Isoda Ryuji, Sasakura Yasunori, Nishikata Takahito, Okano Hideyuki, Makabe Kazuhiro W.

Development Genes and Evolution 210 （ 3 ） 162 - 165 2000年

ISSN  0949-944X

　概要を見る

<p>The gene family encoding RNA-binding proteins includes important regulators involved in the neurogenesis in both protostomes and deuterostomes. We isolated cDNAs of the ascidian homolog of one of the RNA-binding proteins, MUSASHI, from Halocynthia roretzi and Ciona intestinalis. The predicted amino acid sequences contained two RNA-recognition and RNA-binding motifs in the N-terminus and an ascidian-specific YG-rich domain in the C-terminus. Maternal transcripts of musashi were ubiquitous in early cleavage-stage embryos. Ascidian musashi had three domains of zygotic expression: the brain, nerve cord, and mesenchyma. The temporal order of the onset in these domains was highly divergent between the two species of ascidian examined.</p>

Identification and selection of neural progenitor cells

Okano H., Goldman S. A.

NeuroScience News 3 （ 6 ） 27 - 31 2000年

ISSN  1027-6599

　概要を見る

<p>Neural stem cells (NSCs) are self-renewing and multipotential neural progenitor cells, which have received strong attention as potential tools to treat the injured or diseased brain. To develop therapeutic strategies intended to capitalize upon the plasticity and propagability of NSCs, we need both to better understand their biology, and to develop better means for their prospective identification and harvest. We summerize here recent findings from our laboratories. Specifically, we describe the prospective identification of NSCs, using fluorescent reporters placed under the control of the nestin enhancer. Together, these findings provide us both new conceptual and operational strategies for studying NSCs, and as such should greatly accelerate their development for the treatment of neurologic disease.</p>

MSI-1, a neural RNA-binding protein, is involved in male mating behaviour in Caenorhabditis elegans

Yoda Akinori, Sawa Hitoshi, Okano Hideyuki

Genes to Cells 5 （ 11 ） 885 - 895 2000年

ISSN  1356-9597

　概要を見る

<p>Background: Neural RNA-binding proteins are thought to play important roles in neural development and the functional regulation of postmitotic neurones by mediating post-transcriptional gene regulation. RNA-binding proteins belonging to the Musashi family are highly expressed in the nervous system; however, their roles are poorly understood. Results: We identified a Caenorhabditis elegans Musashi homologue, MSI-1, whose RNA-recognition motifs show extensive similarity to those of Drosophila and vertebrate Musashi proteins. We isolated a msi-1 mutant and found males with this mutation to have a mating defect. C. elegans male mating behaviour includes a distinct series of steps: response to contact, backing, turning, vulva location, spicule insertion, and sperm transfer. msi-1 is required for the turning and vulva location steps. Like other Musashi family members, MSI-1 is expressed specifically in neural cells, including male-specific neurones required for turning and vulva location. However, msi-1 was not expressed in proliferating neural progenitors in C. elegans, unlike the Musashi family genes in other systems. Conclusions: Our results suggest that msi-1 is expressed specifically in postmitotic neurones in C. elegans. msi-1 is required for full development of male mating behaviour, possibly through regulation of msi-1 expressing neurones.</p>

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Components of the SWI/SNF complex are required for asymmetric cell division in C. elegans

Sawa Hitoshi, Kouike Hiroko, Okano Hideyuki

Molecular Cell 6 （ 3 ） 617 - 624 2000年

ISSN  1097-2765

　概要を見る

<p>Asymmetric cell division is a fundamental process that produces cellular diversity during development. We have identified two mutants in C. elegans (psa-1 and psa-4) in which the asymmetry of T cell division is disrupted. psa-1 and psa-4 encode homologs of yeast SW13 and SW12/SNF2, respectively, which are components of the SWI/SNF complex. We show by RNA interference assay that homologs of other components of SWI/SNF are also involved in T cell division. psa-1 and psa-4 are likely to be required in the T cell during mitosis to cause asymmetric cell division. Because the SWI/SNF complex is required for asymmetric division in S. cerevisiae, these results demonstrate that at least some aspects of the mechanism of asymmetric cell division are conserved between yeast and a multicellular organism.</p>

Neural stem cells

Okano Hideyuki

Clinical Neurology 41 （ 12 ） 1036 - 1040 2001年12月

ISSN  0009-918X

　概要を見る

<p>Neural stem cells (NSCs) are multipotential progenitor cells that have self-renewal activities. A single NSC is capable of generating various kinds of cells within the CNS, including neurons, astrocytes, and oligodendrocytes. Because of these characteristics, there is an increasing interest in NSCs and neural progenitor cells from the aspects of both basic developmental biology and therapeutic applications to the damaged brain. By understanding of nature of NSCs present in CNS, extracellular factors and signal transduction cascades involved in the differentiation and maintenance of NSCs, population dynamics and localizations of NSCs in embryonic and adult brains, prospective identification and isolation of NSCs, and induction of NSCs into particular neuronal phenotypes, which will be introduced in this review, it would be possible to develop a feasible strategy to manipulate cells in situ to treat the damaged brain.</p>

In vitro selection法によるRNA結合タンパク質の標的配列の同定

今井貴雄，岡野ジェイムス洋尚，岡野栄之

細胞工学 20 （ 12 ） 1690-1697 2001年12月

研究論文（学術雑誌）, 共著

パーキンソン病治療への新たな布石

澤本和延，岡野栄之

Bio ベンチャー 1 （ 3 ） 90-91 2001年11月

研究論文（学術雑誌）, 共著

Role of Deltex-1 as a transcriptional regulator downstream of the Notch receptor

Yamamoto Naoya, Yamamoto Shin-ichi, Inagaki Fuyuki, Kawaichi Masashi, Fukamizu Akiyoshi, Kishi Noriyuki, Matsuno Kenji, Nakamura Kozo, Weinmaster Gerry, Okano Hideyuki, Nakafuku Masato

Journal of Biological Chemistry 276 （ 48 ） 45031-45040 2001年11月

研究論文（学術雑誌）, 共著

再生治療の海外基礎研究開発状況―特に幹細胞生物学研究を中心に

金村米博，岡野栄之

Science&Technology Jounal 10 （ 00 ） 14-17 2001年11月

研究論文（学術雑誌）, 共著

神経幹細胞の同定・分離の確立と神経再生への挑戦

岡野栄之

神経治療学 18 （ 5/6合併号 ） 443-449 2001年11月

研究論文（学術雑誌）, 単著

神経幹細胞と再生

今井貴雄，岡野ジェイムス洋尚，岡野栄之

治療学 35   29-35 2001年10月

研究論文（学術雑誌）, 共著

RNA-binding protein Musashi2: Developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS

Sakakibara Shin-ich, Nakamura Yuki, Satoh Hitoshi, Okano Hideyuki

Journal of Neuroscince 21 （ 20 ） 8091-8107 2001年10月

研究論文（学術雑誌）, 共著

再生医学の基本戦略：中枢神経系を中心として

岡野栄之

KAST Report 13 （ 1 ） 24-29 2001年10月

研究論文（学術雑誌）, 単著

神経幹細胞：その基礎生物学と高次神経機能・精神神経疾患への応用

岡野栄之

精神神経学雑誌 103 （ 10 ） 759-763 2001年10月

研究論文（学術雑誌）, 単著

幹細胞システムを用いた中枢神経系の再生医学

岡野栄之

Neurologia medico-chirurgica 41 （ extra issue ） 23 2001年10月

研究論文（学術雑誌）, 単著

Brain from bone:Efficient'meta-differentiation'of marrow stroma-derived mature osteoblasts to neurons with Noggin or a demethylating agent

Jun Kohyama, Hitoshi Abe, Takuya Shimazaki, Amane Koizumi, Kin-ichi Nakashima, Satoshi Gojo, Tetsuya Taga, Hideyuki Okano, Jun-ichi Hata, Akihiro Umezawa

Differentiation 68 （ 4-5 ） 235-244 2001年10月

研究論文（学術雑誌）, 共著

IP₃ receptor type 1 mRNAの3'末端非翻訳領域に結合する遺伝子産物と小脳purkinje細胞におけるmRNA局在化機構

飯島崇利，今井貴雄，木村有喜，Bernstein Alan，岡野栄之

神経化学 40 （ 2,3 (Suppl) ） 311 2001年09月

研究論文（学術雑誌）, 共著

An imaging-based 'time-table' of melecular expression in cultured murine neuroepthelial cells

Kawaguchi Ayano, Ogawa Maaharu, Okano Hideyuki, Saito Kaneko, Miyata Takayuki

Neurosciene Research 25 （ Suppl ） S128 2001年09月

研究論文（学術雑誌）, 共著

High-yield selection and extraction of two promoter-defined phenotypes of neural stem cells from the fetal human brain

Keyoung Michael H, Roy Neeta S, Benraiss Abdellatif, Louissaint Abner Jr, Suzuki Akira, Hashimoto Mitsuhiro, Rashbaum William K, Okano Hideyuki, Goldman Steven A

Nature Biotechnology 19 （ 9 ） 843-850 2001年09月

研究論文（学術雑誌）, 共著

Asymmetric inheritance of radial glial fibers by cortical neurons

Miyata Takaki, Kawaguchi Ayano, Okano Hideyuki, Ogawa Masaharu

Neuron 31 （ 5 ） 727-741 2001年09月

研究論文（学術雑誌）, 共著