論文 - 岡野　栄之

Hzf regulates adipogenesis through translational control of C/EBP alpha

岡野 栄之

EMBO JOURNAL 27 （ 10 ） 1481 - 1490 2008年05月

研究論文（学術雑誌）,  ISSN  0261-4189

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Role of IL-6 in regulation of inflammation and stem cell differentiation in CNS trauma

Mukaino Masahiko, Nakamura Masaya, Okada Seiji, Toyama Yoshiaki, Liu Meigen, Okano Hideyuki

Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology 31 （ 2 ） 93 - 98 2008年04月

ISSN  0911-4300

　概要を見る

<p>Interleukin-6 (IL-6) has been demonstrated to play a pivotal role in CNS trauma as a proinflammatory cytokine which regulates inflammatory response. Not only being a mediator of inflammation, but IL-6 induces neural stem/progenitor cells to undergo astrocytic differentiation selectively in the injured CNS. These effects are considered to coordinate to prevent the CNS repair after traumatic injury. Consistently, we previously reported that the administration of anti-IL-6 receptor antibody (MR16-1) immediately after spinal cord injury in mice decreased the number of invading inflammatory cells and the severity of connective tissue scar formation, and led to improved functional recovery. These findings suggest that neutralization of IL-6 signaling in the acute phase of SCI will be beneficial for CNS repair. A critical merit of this anti-IL-6 receptor antibody is that humanized antibody to human IL-6 receptor (MRA; Atlizumab) has already been reshaped. However, there are several studies which show beneficial aspects of IL-6 signaling in the pathology of CNS trauma. Further investigation of the mechanisms how MR16-1 reduces tissue damage should be required for clinical application.</p>

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Isolation and characterization of dendritic cells from common marmosets for preclinical cell therapy studies

岡野 栄之

IMMUNOLOGY 123 （ 4 ） 566 - 574 2008年04月

研究論文（学術雑誌）,  ISSN  0019-2805

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Spatial gradient effects of 120 mT static magnetic field on endothelial tubular formation in vitro

岡野 栄之

BIOELECTROMAGNETICS 29 （ 3 ） 233 - 236 2008年04月

研究論文（学術雑誌）,  ISSN  0197-8462

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M-numb and notch signals are the key factors for gastric mucosal regeneration after acute damage

岡野 栄之

GASTROENTEROLOGY 134 （ 4 ） A235 - A235 2008年04月

研究論文（学術雑誌）,  ISSN  0016-5085

Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors

岡野 栄之

JOURNAL OF CELL SCIENCE 121 （ 8 ） 1204 - 1212 2008年04月

研究論文（学術雑誌）,  ISSN  0021-9533

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Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad

岡野 栄之

CELL STEM CELL 2 （ 4 ） 392 - 403 2008年04月

研究論文（学術雑誌）,  ISSN  1934-5909

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[Mammalian neural stem cells].

Shimazaki Takuya, Okano Hideyuki

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 53 （ 4 Suppl ） 311 - 317 2008年03月

ISSN  0039-9450

Enhanced transfection efficiency in laser-induced stress wave-assisted gene transfer at low laser fluence by increasing pressure impulse

岡野 栄之

APPLIED PHYSICS EXPRESS 1 （ 3 ） S89 - S89 2008年03月

研究論文（学術雑誌）,  ISSN  1882-0778

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[Strategies for axonal regeneration after spinal cord injury].

Kitamura Kazuya, Nakamura Masaya, Toyama Yoshiaki, Okano Hideyuki

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 53 （ 4 Suppl ） 411 - 417 2008年03月

ISSN  0039-9450

Helicobacter pylori infection induces candidate stem cell marker musashi-1 in the human gastric epithelium

岡野 栄之

DIGESTIVE DISEASES AND SCIENCES 53 （ 2 ） 363 - 369 2008年02月

研究論文（学術雑誌）,  ISSN  0163-2116

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The intestinal epithelium compensates for p53-mediated cell death and guarantees organismal survival

Valentin-Vega Y. A., Okano H., Lozano G.

Cell Death and Differentiation 15 （ 11 ） 1772 - 1781 2008年

ISSN  1350-9047

　概要を見る

<p>Mdm2 is the major inhibitor of the p53 tumor suppressor. Loss of Mdm2 in mice or in specific tissues of the mouse always yields p53-dependent lethal phenotypes. However, the role of Mdm2 in tissues with high turnover capacity is unknown. We have engineered mice lacking Mdm2 in the intestinal epithelium using the Cre/LoxP system. Loss of Mdm2 (Mdm2^intΔ) results in viable animals, but neonates display multiple intestinal abnormalities such as hyperplasia, enterocyte vacuolization, and inflammation. These defects correlate with a drastic increase in p53-dependent apoptosis in highly proliferative and differentiated cells. Unexpectedly, the observed phenotypes disappear with age. The tissue selects against Mdm2-null cells and increases its proliferative capacity. Additionally, the intestinal stem and progenitor cell populations are enriched leading to an increase in crypt fission events. Enhanced proliferation is achieved by activation of the canonical Wnt and EGFR-mediated Ras/MAPK pathways. While Mdm2 is a critical inhibitor of p53 in the intestinal epithelium, the tissue employs a series of processes that compensate for cell death.</p>

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Anti IL-6 antibody treatment reduces the infiltration of hematogenous macrophages into the injured spinal cord

岡野 栄之

NEUROSCIENCE RESEARCH 61   S275 - S275 2008年

研究論文（学術雑誌）,  ISSN  0168-0102

A role of neuronal RNA-binding protein Hu and NF45/90 in the nervous system

岡野 栄之

NEUROSCIENCE RESEARCH 61   S153 - S153 2008年

研究論文（学術雑誌）,  ISSN  0168-0102

Expression of free fatty acid receptor GPR40 in the neurogenic niche of adult monkey hippocampus

岡野 栄之

HIPPOCAMPUS 18 （ 3 ） 326 - 333 2008年

研究論文（学術雑誌）,  ISSN  1050-9631

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Mature Adipocyte-Derived Cells, Dedifferentiated Fat Cells (DFAT), Promoted Functional Recovery From Spinal Cord Injury-Induced Motor Dysfunction in Rats

岡野 栄之

CELL TRANSPLANTATION 17 （ 8 ） 877 - 886 2008年

研究論文（学術雑誌）,  ISSN  0963-6897

In vivo imaging in humanized mice

Masuda H., Okano H. J., Maruyama T., Yoshimura Y., Okano H., Matsuzaki Y.

Current topics in microbiology and immunology 324   179 - 196 2008年

ISSN  0070-217X

　概要を見る

<p>The radiological modalities that are currently utilized as critical components in clinical medicine have also been adapted to small-animal imaging, among which are ultrasound imaging, X-ray computerized tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and singlephoton emission computed tomography (SPECT). Optical imaging techniques such as bioluminescence imaging (BLI) and fluorescence imaging (FLI) are approaches that are commonly used in small animals. Longitudinal surveys of living (i.e., nonsacrificed) animal models with these modalities provide some clues for the development of clinical applications. The techniques are absolutely essential for translational research. However, there are currently few tools available with sufficient spatial or temporal resolution ideal for all experimental studies. In this chapter, we provide a rationale and techniques for visualizing target cells in living small animals and an overview of the advantages and limitations of current imaging technology. Finally, we introduce a humanized mouse and a novel in vivo imaging system that we have developed. We also discuss real-time observations of reconstructs and clinical manifestations. © 2008 Springer-Verlag.</p>

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Structural analysis of Musashi-RNA complex on the basis of long-range structural information.

Ohyama Takako, Furukawa Ayako, Mashima Tsukasa, Sugiyama Takashi, Ohgara Shouta, Yamazaki Toshio, Imai Takao, Okano Hideyuki, Nagata Takashi, Katahira Masato

Nucleic acids symposium series (2004)  （ 52 ） 193 - 194 2008年

　概要を見る

<p>Musashi protein is supposed to be involved in the regulation of differentiation of neural stem cells. Musashi binds to 3' untranslated region of target mRNA and represses the translation of mRNA. Musashi has two tandem RNA-binding domains (RBDs), RBD1 and RBD2. Both RBDs cooperatively bind to the target mRNA. Here, we determined the structure of RBD1-RBD2 in complex with target RNA. First, the structures of two RBDs in the complex were determined on the basis of short-range distance restrains derived from NOEs. However, the relative position of two RBDs was not determined due to the lack of long-range distance restraints across two RBDs. In order to overcome the situation, we introduced the paramagnetic center into Musashi by attaching MTSL carrying the NO radical. The long-range distance restraints (ca. 20-40 A) between two RBDs were derived from paramagnetic relaxation enhancement (PRE) caused by the paramagnetic center. The relative position of two RBDs was successfully determined on the basis of these long-range distance restraints. The change in the relative position of two RBDs on binding to the target RNA was also detected by PRE. The determined structure of RBD1-RBD2 in the complex has suggested how Musashi recognizes its target mRNA.</p>

Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats

Ohta Yuki, Takenaga Mitsuko, Tokura Yukie, Hamaguchi Akemi, Matsuraoto Taro, Kano Koichiro, Mugishima Hideo, Okano Hideyuki, Igarashi Rie

Cell Transplantation 17 （ 8 ） 877 - 886 2008年

ISSN  0963-6897

　概要を見る

<p>Transplantation of mature adipocyte-derived cells (dedifferentiated fat cells) led to marked functional recovery from spinal cord injury (SCI)-induced motor dysfunction in rats. When mature adipocytes were isolated from rat adipose tissue and grown in ceiling culture, transformation into fibroblast-like cells without lipid droplets occurred. These fibroblast-like cells, termed dedifferentiated fat cells (DFAT), could proliferate and could also differentiate back into adipocytes. DFAT expressed neural markers such as nestin, βIII tubulin, and GFAP. Allografting of DFAT into SCI-induced rats led to significant recovery from hindlimb dysfunction. Grafted cells were detected at the injection site, and some of these cells expressed βIII tubulin. DFAT expressed neurotrophic factors such as BDNF and GDNF prior to transplantation, and grafted cells were also positive for these factors. Therefore, these neurotrophic factors derived from grafted DFAT might have contributed to the promotion of functional recovery. These findings also suggest that mature adipocytes could become a new source for cell replacement therapy to treat central nervous system disorders. Copyright © 2008 Cognizant Comm. Corp.</p>

Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP

岡野 栄之

NEUROSCIENCE RESEARCH 61   S153 - S153 2008年

研究論文（学術雑誌）,  ISSN  0168-0102