論文 - 橋口　明典

腎生検所見から何を学ぶか(No.53)　タクロリムスが上皮下沈着物に対して治療効果を示した難治性ループス腎炎の1例

野中, 香苗, 乳原, 善文, 諏訪部, 達也, 住田, 圭一, 平松, 里佳子, 長谷川, 詠子, 豊原, 敬文, 広浜, 大五郎, 山内, 真之, 山内, 淳司, 早見, 典子, 冨永, 直人, 星野, 純一, 澤, 直樹, 竹本, 文美, 黒田, 陽子, 大橋, 健一, 高市, 憲明 and 橋口, 明典

腎と透析 77 （ 3 ） 389 - 398 2014年09月

共著,  ISSN  0385-2156

BCG膀胱内注入療法により誘発された腎サルコイドーシスの一例

新井 舞, 柏 真紀, 徳山 博文, 脇野 修, 橋口 明典, 林 松彦, 小西 孝之助, 林 晃一, 伊藤 裕

日本腎臓学会誌 （(一社)日本腎臓学会）  56 （ 6 ） 845 - 845 2014年08月

共著,  ISSN  0385-2385

急性骨髄性白血病(AML)に対して造血幹細胞移植施行後に微小血管病変(TMA)を発症した一例

浦井 秀徳, 徳山 博文, 脇野 修, 橋口 明典, 小西 孝之助, 林 晃一, 伊藤 裕

日本腎臓学会誌 （(一社)日本腎臓学会）  56 （ 6 ） 846 - 846 2014年08月

共著,  ISSN  0385-2385

ステロイドが著効した間質性腎炎を伴うLight chain tubulopathyの一例

内藤 真規子, 脇野 修, 橋口 明典, 徳山 博文, 小西 孝之助, 林 晃一, 林 松彦, 伊藤 裕

日本腎臓学会誌 （(一社)日本腎臓学会）  56 （ 6 ） 840 - 840 2014年08月

共著,  ISSN  0385-2385

HE染色肝病理標本における組織構造定量化によるがん検出精度向上の検討

石川, 雅浩, 藤田, 悠介, Aziz, Maulana Abdul, 村上, 百合, 山口, 雅浩, 小林, 直樹, 喜友名, 朝春, 齋藤, 彰, 阿部, 時也, 橋口, 明典 and 坂元, 亨宇

MEDICAL IMAGING TECHNOLOGY 32 （ Suppl. ） 1 - 9 2014年07月

共著,  ISSN  0288-450X

　概要を見る

HE染色病理組織標本におけるコンピュータ診断支援の従来研究では、細胞核の形態的特徴を用いる場合が多いが、特に高分化がんあるいは早期がんの検出、診断においては核情報以上に組織構造が重要な役割を持っている。本発表では、肝細胞がんを対象として、これまでに我々が検討を行ってきた組織構造の特徴量をがんの識別に応用した基礎的な結果を報告する。具体的には、肝細胞索の層の厚さ、索構造の乱れ、肝細胞索ごとに算出したN/C比及び核密度などを画像から計測した結果、がんと非がんの間で有意差が認められた。さらに、これらの組織構造の特徴量を従来の細胞核の特徴量に加えることにより、がん識別の精度を向上する可能性が示された。本手法で用いた組織構造の特徴量は、病理組織標本のデジタルスライド画像から自動的に算出できるもので、定量的情報に基づくコンピュータ病理診断支援システムに有用なものと考えられる。(著者抄録)

Effective Color Transform Method for Image Compression of H&E Stained Whole Slide Image Using Subset Pixels

KOBAYASHI Naoki, SHINODA Kazuma, KATOH Ayako, KOMAGATA Hideki, YAMAGUCHI Masahiro, ABE Tokiya, HASHIGUCHI Akinori, SAKAMOTO Michiie

IIEEJ Trans Image Electron Vis Comput (CD-ROM) （The Institute of Image Electronics Engineers of Japan）  2 （ 1 ） 37 - 47 2014年06月

共著, 査読有り,  ISSN  2188-191X

• CiNii Research

抗GBM抗体陽性腎炎発症し、透析導入後にヘノッホ・シェーライン紫斑病(HSP)を併発した一例

小口 英世, 徳山 博文, 脇野 修, 小西 孝之助, 橋口 明典, 林 晃一, 伊藤 裕

日本透析医学会雑誌 （(一社)日本透析医学会）  47 （ Suppl.1 ） 1012 - 1012 2014年05月

共著,  ISSN  1340-3451

肥満関連腎症 組織学的変化の新たな知見

徳山 博文, 脇野 修, 橋口 明典, 小西 孝之助, 林 晃一, 伊藤 裕

日本腎臓学会誌 （(一社)日本腎臓学会）  56 （ 3 ） 249 - 249 2014年05月

共著,  ISSN  0385-2385

Whole slide imageを用いた膠原・弾性線維定量法

阿部, 時也, 橋口, 明典, 山崎, 剣 and 坂元, 亨宇

病理と臨床 32 （ 4 ） 448 - 450 2014年04月

共著,  ISSN  0287-3745

Distinct Cell Clusters Touching Islet Cells Induce Islet Cell Replication in Association with Over-Expression of Regenerating Gene (REG) Protein in Fulminant Type 1 Diabetes

橋口 明典

PLOS ONE （PUBLIC LIBRARY SCIENCE）  9 （ 4 ） 774 - 779 2014年04月

研究論文（学術雑誌）, 共著,  ISSN  1932-6203

　概要を見る

Background: Pancreatic islet endocrine cell-supporting architectures, including islet encapsulating basement membranes (BMs), extracellular matrix (ECM), and possible cell clusters, are unclear.
Procedures: The architectures around islet cell clusters, including BMs, ECM, and pancreatic acinar-like cell clusters, were studied in the non-diabetic state and in the inflamed milieu of fulminant type 1 diabetes in humans.
Result: Immunohistochemical and electron microscopy analyses demonstrated that human islet cell clusters and acinar-like cell clusters adhere directly to each other with desmosomal structures and coated-pit-like structures between the two cell clusters. The two cell-clusters are encapsulated by a continuous capsule composed of common BMs/ECM. The acinar-like cell clusters have vesicles containing regenerating (REG) I alpha protein. The vesicles containing REG I alpha protein are directly secreted to islet cells. In the inflamed milieu of fulminant type 1 diabetes, the acinar-like cell clusters over-expressed REG I alpha protein. Islet endocrine cells, including beta-cells and non-beta cells, which were packed with the acinar-like cell clusters, show self-replication with a markedly increased number of Ki67-positive cells.
Conclusion: The acinar-like cell clusters touching islet endocrine cells are distinct, because the cell clusters are packed with pancreatic islet clusters and surrounded by common BMs/ECM. Furthermore, the acinar-like cell clusters express REG I alpha protein and secrete directly to neighboring islet endocrine cells in the non-diabetic state, and the cell clusters over-express REG Ia in the inflamed milieu of fulminant type 1 diabetes with marked self-replication of islet cells.

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Supratentorial neurenteric cyst with spontaneous repetitive intracystic hemorrhage mimicking brain abscess: a case report

橋口 明典

NEUROSURGICAL REVIEW （SPRINGER）  37 （ 1 ） 153 - 159 2014年01月

研究論文（学術雑誌）, 共著,  ISSN  0344-5607

　概要を見る

Neurenteric cyst (NC) is a benign epithelial cyst (BEC) of endodermal origin that mostly occurs in the spinal subdural space or posterior cranial fossa. A 28-year-old male presented with a left frontal lobe NC associated with spontaneous repetitive intracystic hemorrhage, which was initially diagnosed and treated as a brain abscess. He presented with headache and disorientation, without underlying diseases. A cystic tumor was suspected because of a hypointense signal on diffusion-weighted magnetic resonance imaging (MRI). One day after admission, his condition deteriorated rapidly and emergency cyst aspiration was performed. A brown viscous liquid like bloody pus comprising many neutrophils and macrophages was obtained. Although culture was negative, we initially started antibiotic treatment because of cyst content characteristics and rapid clinical course compatible with brain abscess. He was discharged without neurological deficits, but occasionally complained of intense headache. Computed tomography/MRI showed repetitive intracystic hemorrhage and gradual re-enlargement of the lesion. He underwent radical cyst excision by frontal craniotomy 34 months after aspiration. The pathological diagnosis was NC. We believe this is the first report of a supratentorial NC with spontaneous repetitive intracystic hemorrhage. BECs, especially with intracystic hemorrhage, are difficult to be distinguished from brain abscesses. In cases of cystic lesions or presumed brain abscesses refractory to treatment with aspiration and/or antibiotics, BECs should be considered, and radical cyst wall removal should be considered a treatment option.

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Inhibition of Activated Rac1 Signaling Induces Recovery of E-Cadherin Function and Abrogation of Invasive Phenotype of Side Population in Colon Cancer Cells

橋口 明典

DIGESTION （KARGER）  89 （ 1 ） 83 - 83 2014年

研究論文（学術雑誌）, 共著,  ISSN  0012-2823

Enhancing Automatic Classification of Hepatocellular Carcinoma Images through Image Masking, Tissue Changes, and Trabecular Features

橋口 明典

ANALYTICAL CELLULAR PATHOLOGY （Hindawi Limited）  2014   51 - 58 2014年

研究論文（学術雑誌）, 共著,  ISSN  2210-7177

• Access to Document (DOI)

Whole Slide Image Analysis System for Quantification of Liver Fibrosis

橋口 明典

ANALYTICAL CELLULAR PATHOLOGY （Hindawi Limited）  2014   774 - 779 2014年

研究論文（学術雑誌）, 共著,  ISSN  2210-7177

• Access to Document (DOI)

Development of a Prototype for Hepatocellular Carcinoma Classification Based on Morphological Features Automatically Measured in Whole Slide Images

橋口 明典

ANALYTICAL CELLULAR PATHOLOGY （Hindawi Limited）  2014   774 - 779 2014年

研究論文（学術雑誌）, 共著,  ISSN  2210-7177

• Access to Document (DOI)

Computational hepatocellular carcinoma tumor grading based on cell nuclei classification

Atupelage, C., Nagahashi, H., Kimura, F., Yamaguchi, M., Tokiya, A., Hashiguchi, A., Sakamoto, M.

Journal of Medical Imaging 1 （ 3 ） 034501 - 034501 2014年

共著, 査読有り

• Access to Document (DOI)

Color processing in pathology image analysis system for liver biopsy

Yuri Murakami, Tokiya Abe, Yoshiko Yamashita, Masahiro Yamaguchi, Masahiro Ishikawa, Akinori Hashiguchi, Kiyuna Tomoharu, Akira Saito, Michiie Sakamoto

Final Program and Proceedings - IS and T/SID Color Imaging Conference （Society for Imaging Science and Technology）  2014- （ January ） 184 - 188 2014年

共著,  ISSN  2169-2629

　概要を見る

We have been developing a prototype system for the automatic detection of hepatocellular carcinoma (HCC) from whole slide images (WSIs) of liver biopsy based on image analysis techniques. In this paper, we present two color-related topics of this system: color correction and the calculation of color-related features of cytoplasm. A WSI-basis color correction method was implemented for the prototype system. We tested the color correction using more than 300 WISs, and it was confirmed that the color correction works well and stably. In addition, it was found that the success rate of nuclei detection significantly increaseed due to color correction. As for color-related features, we propose a method to calculate the representative color of cytoplasm and the clearness of cytoplasm. It was found that there was slight difference between the distributions of the representative colors of HCC and non-cancer tissues. In addition, there was slight correlation between the clearness of cytoplasm and nuclei density, which implies a promising role of the clearness index in a nuclei-based HCC detection.

Renal thrombotic microangiopathy in a patient with septic disseminated intravascular coagulation

橋口 明典

BMC NEPHROLOGY （BIOMED CENTRAL LTD）  14   3037 - 3045 2013年11月

研究論文（学術雑誌）, 共著,  ISSN  1471-2369

　概要を見る

Background: The mechanism for the development of thrombotic microangiopathy (TMA) during sepsis has only been partially elucidated. TMA is recognized as a disease caused by various factors, and may be involved in the emergence of organ damage in severe sepsis. Here we report a case of TMA that followed disseminated intravascular coagulation (DIC) due to severe infection in a patient with a reduced ADAMTS-13 activity level.
Case presentation: An 86-year-old Japanese woman was admitted to our hospital because of low back pain and fever. A careful evaluation led to a diagnosis of acute obstructive pyelonephritis due to a ureteral stone. Proteus mirabilis was isolated from both blood and urine cultures. The patient developed systemic inflammatory response syndrome and DIC, and was treated with antibiotics and daily continuous hemodiafiltration. Although infection and the coagulation abnormalities due to DIC were successfully controlled, renal failure persisted and her consciousness level deteriorated progressively in association with severe thrombocytopenia and microangiopathic hemolytic anemia. We therefore suspected the presence of TMA and started plasma exchange, which resulted in an impressive improvement in consciousness as well as the laboratory abnormalities. The ADAMTS-13 activity was 44% and the patient tested negative for the ADAMTS-13 inhibitor prior to the initiation of plasma exchange. A renal biopsy was performed to determine the etiology of acute renal injury, which revealed findings that were interpreted to be compatible with the sequelae of TMA. The follow-up studies performed after the successful treatment of TMA showed that her plasma ADAMTS-13 activity level remained persistently low. It is surmised that septic DIC occurring in the presence of preexisting reduced ADAMTS-13 activity have led to the development of secondary TMA in the present case.
Conclusion: The present case suggests that TMA can be superimposed on sepsis-induced DIC, and plasma exchange is expected to be beneficial in such situations. Clinicians should consider the possibility of secondary TMA that follows sepsis-induced DIC in certain indicative clinical settings.

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IgA腎症の寛解基準の提唱

松尾, 清一, 川村, 哲也, 鈴木, 祐介, 城, 謙輔, 富野, 康日己, 堀越, 哲, 西野, 友哉, 吉川, 徳茂, 服部, 元史, 木村, 健二郎, 安田, 隆, 白井, 小百合, 柴田, 孝則, 吉村, 光弘, 宇都宮, 保典, 遠藤, 正之, 坂本, なほ子, 松島, 雅人, 宮崎, 陽一, 安田, 宜成, 横尾, 隆, 香美, 祥二, 幡谷, 浩史, 鈴木, 仁, 松崎, 慶一, 内田, 俊也, 伊藤, 孝史, 清水, 章, 片渕, 律子, 久野, 敏, 橋口, 明典 and 厚生労働科学研究費補助金難治性疾患克服研究事業進行性腎障害に関する調査研究班報告IgA腎症分科会

日本腎臓学会誌 （(一社)日本腎臓学会）  55 （ 7 ） 1249 - 1254 2013年10月

共著,  ISSN  0385-2385

Clinical significance of quantitative measurement of elastin in liver biopsy

橋口 明典

HEPATOLOGY 58   605A - 605A 2013年10月

研究論文（学術雑誌）, 共著,  ISSN  0270-9139