Papers - Hashiguchi, Akinori

An accurate method of extracting fat droplets in liver images for quantitative evaluation

Ishikawa, Masahiro, Kobayashi, Naoki, Komagata, Hideki, Shinoda, Kazuma, Yamaguchi, Masahiro, Abe, Tokiya, Hashiguchi, Akinori, Sakamoto, Michiie

Medical Imaging 2015: Digital Pathology 9420 2015

Joint Work, Accepted

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Enhancing automatic classification of hepatocellular carcinoma images through image masking, tissue changes and trabecular features.

Aziz, Maulana Abdul, Kanazawa, Hiroshi, Murakami, Yuri, Kimura, Fumikazu, Yamaguchi, Masahiro, Kiyuna, Tomoharu, Yamashita, Yoshiko, Saito, Akira, Ishikawa, Masahiro, Kobayashi, Naoki, Abe, Tokiya, Hashiguchi, Akinori, Sakamoto, Michiie

Journal of pathology informatics 6   26 - 26 2015

Joint Work, Accepted

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Overexpression of CXCR4 on circulating B cells in patients with active systemic lupus erythematosus

Hironari Hanaoka, Yuka Okazaki, Akinori Hashiguchi, Hidekata Yasuoka, Tsutomu Takeuchi, Masataka Kuwana

Clinical and Experimental Rheumatology (Clinical and Experimental Rheumatology S.A.S.)  33 ( 6 ) 863 - 870 2015

Joint Work,  ISSN  1593-098X

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Objective: To evaluate the roles of circulating B cells in the pathogenic process of systemic lupus erythematosus (SLE) by measuring the expression of chemokines and their receptors. Methods: Peripheral-blood mononuclear cells were obtained from 17 active, 21 inactive SLE patients, and 13 healthy controls. The expression of CXCR4, CXCR5, and CCR7 on CD19+ B cells was determined by flow cytometry, serum concentration of CXCL12 was measured by enzyme-linked immunosorbent assay, and the chemotactic responsiveness of B cells toward CXCL12 was evaluated. B or plasma cells expressing CXCR4 in renal biopsy specimens were detected using immnofluorescent staining. Results: Flow cytometric analysis revealed that expression level of CXCR4 on circulating B cells was significantly higher in patients with active disease than in those with inactive disease or controls. Serum CXCL12 concentration was not different between these groups. In addition, the migratory ability of B cells toward CXCL12 was enhanced in active SLE patients. Finally, CXCR4-expressing B cells were more frequently observed in the renal biopsy specimens of lupus nephritis. Conclusion: Up-regulated CXCR4 expression on circulating B cells in active SLE may enhance their chemotactic response toward CXCL12, which may promote infiltration of these cells into inflamed renal tissue and contribute to the development of SLE.

Improvement in karyomegalic interstitial nephritis three years after ifosfamide and cisplatin therapy by corticosteroid

Matsuura, T, Wakino, S, Yoshifuji, A, Nakamura, T, Tokuyama, H, Hashiguchi, A, Konishi, K, Iwasa, T, Shoji, M, Hosono, A, Ohashi, K, Chuman, H, Itoh, H

CEN Case Rep 3 ( 2 ) 226 - 231 2014.11

Joint Work,  ISSN  2192-4449

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Long-term nephrotoxicity of ifosfamide is occasionally progressive, and, in such case, there has been no specific treatment to prevent progression. It has been reported that the presence of karyomegalic interstitial nephritis, which is rare type of interstitial nephritis, may be related to ifosfamide-induced nephropathy with poor prognosis and resistant to the immunosuppressive therapy. A 15-year-old boy presented with progressive nephrotoxicity 3 years after systemic chemotherapy with ifosfamide and cisplatin for the treatment of osteosarcoma. Renal biopsy revealed the severe tubulointerstitial nephritis with tubular atrophy and focal global and segmental glomerular sclerosis. It also showed tubular epithelial cells with variably sized nuclei, some of which were massively enlarged, abnormal hyperchromatic, irregular shaped, and bizarre-appearing. These morphological changes were suggestive of the histology of karyomegalic interstitial nephritis. Corticosteroid retarded the progression of nephrotoxicity. The present case is the first report, suggesting that corticosteroid was effective against the late-onset renal toxicity by ifosfamide therapy. Our case also suggests that karyomeg

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Up-Regulated Expression of CXCR4 on Circulating B Cells in Patients with Systemic Lupus Erythematosus

Hanaoka, Hironari, Okazaki, Yuka, Hashiguchi, Akinori, Yasuoka, Hidekata, Takeuchi, Tsutomu, Kuwana, Masataka

ARTHRITIS & RHEUMATOLOGY (WILEY-BLACKWELL)  66   S1177 - S1178 2014.10

Research paper (scientific journal), Joint Work,  ISSN  2326-5191

Rac1 accumulates in the nucleus at the invasive front of colorectal cancer through promoting cell motility

Funakoshi, Shinsuke, Takaishi, Hiromasa, Niibe-Kabashima, Ayano, Suzuki, Tomohiro, Sakai, Gen, Izumiya, Motoko, Adachi, Masayuki, Hamamoto, Yasuo, Higuchi, Hajime, Hashiguchi, Akinori, Tsukada, Nobuhiro, Kanai, Takanori

CANCER RESEARCH (AMER ASSOC CANCER RESEARCH)  74 ( 19 ) 865 - 870 2014.10

Research paper (scientific journal), Joint Work,  ISSN  0008-5472

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A rare association between Fabry's disease and granulomatosis with polyangiitis: a potential pathogenic link

Hironari Hanaoka, Akinori Hashiguchi, Konosuke Konishi, Tomohiro Ishii, Masataka Kuwana

BMC NEPHROLOGY (BIOMED CENTRAL LTD)  15   157 2014.10

Joint Work, Accepted,  ISSN  1471-2369

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Background: Fabry's disease is a rare X-linked, hereditary lysosomal storage disease caused by a deficiency of the enzyme alpha-galactosidase A. Granulomatosis with polyangiitis is characterized by the involvement of the respiratory tract and kidneys. Here, we report the first case of the coexistence of these diseases.
Case presentation: We describe a 29-year-old man suffering from fever with maxillary sinusitis, multiple lung nodules, and proteinuria. He was diagnosed with Fabry's disease accompanying granulomatosis with polyangiitis on the basis of the low activity of peripheral leukocyte alpha-galactosidase A and pathological findings in the lung and kidney. Glucocorticoid and cyclophosphamide were administered, followed by enzyme replacement therapy. Progression to end-stage renal disease has not been observed for 6 years until the time of drafting this manuscript.
Conclusion: Because both Fabry's disease and granulomatosis with polyangiitis or crescentic glomerulonephritis are rare diseases, their concurrence in this and related cases suggests there may be a pathogenic link between these two conditions. Fabry's disease may be underdiagnosed, particularly in cases of granulomatosis with polyangiitis or crescentic glomerulonephritis.

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Label-free visualization of acetaminophen-induced liver injury by high-speed stimulated Raman scattering spectral microscopy and multivariate image analysis

Shuya Satoh, Yoichi Otsuka, Yasuyuki Ozeki, Kazuyoshi Itoh, Akinori Hashiguchi, Ken Yamazaki, Hiroyuki Hashimoto, Michiie Sakamoto

PATHOLOGY INTERNATIONAL (WILEY-BLACKWELL)  64 ( 10 ) 518 - 526 2014.10

Joint Work, Accepted,  ISSN  1320-5463

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We recently established a high-speed, label-free, spectral imaging method based on stimulated Raman scattering (SRS). This method enables examination of cellular features within relatively short periods, thus enabling new imaging applications in pathology. Previously, we reported on label-free visualization of mouse tissue using SRS spectral microscopy combined with multivariate image analysis, but the feasibility of applying this approach to diseased tissues with diverse morphology and irregular chemical species has not been examined. We, therefore, assessed acetaminophen-induced liver injury to evaluate the potential use of Raman spectral microscopy for visualizing histopathologic specimens. Acetaminophen-overdosed mouse liver was prepared and the pathologic changes including centrilobular necrosis were confirmed. Multi-colored images were reconstructed through principal component analysis (PCA) of a multi-band SRS dataset, which provided rich information compared with a monochrome single-band SRS dataset. A wide view of the multi-colored principal component (PC) images showed the distribution of cellular constituents, which was similar to that observed by fat staining. In addition, different types of cells in liver parenchyma were also demonstrated. In conclusion, the combination of SRS spectral microscopy and PCA has the potential to reveal both the morphological and chemical features of specimens and therefore has potential utility in diagnostic pathology.

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腎生検所見から何を学ぶか(No.53) タクロリムスが上皮下沈着物に対して治療効果を示した難治性ループス腎炎の1例

野中, 香苗, 乳原, 善文, 諏訪部, 達也, 住田, 圭一, 平松, 里佳子, 長谷川, 詠子, 豊原, 敬文, 広浜, 大五郎, 山内, 真之, 山内, 淳司, 早見, 典子, 冨永, 直人, 星野, 純一, 澤, 直樹, 竹本, 文美, 黒田, 陽子, 大橋, 健一, 高市, 憲明, 橋口, 明典

腎と透析 77 ( 3 ) 389 - 398 2014.09

Joint Work,  ISSN  0385-2156

BCG膀胱内注入療法により誘発された腎サルコイドーシスの一例

新井 舞, 柏 真紀, 徳山 博文, 脇野 修, 橋口 明典, 林 松彦, 小西 孝之助, 林 晃一, 伊藤 裕

日本腎臓学会誌 ((一社)日本腎臓学会)  56 ( 6 ) 845 - 845 2014.08

Joint Work,  ISSN  0385-2385

急性骨髄性白血病(AML)に対して造血幹細胞移植施行後に微小血管病変(TMA)を発症した一例

浦井 秀徳, 徳山 博文, 脇野 修, 橋口 明典, 小西 孝之助, 林 晃一, 伊藤 裕

日本腎臓学会誌 ((一社)日本腎臓学会)  56 ( 6 ) 846 - 846 2014.08

Joint Work,  ISSN  0385-2385

ステロイドが著効した間質性腎炎を伴うLight chain tubulopathyの一例

内藤 真規子, 脇野 修, 橋口 明典, 徳山 博文, 小西 孝之助, 林 晃一, 林 松彦, 伊藤 裕

日本腎臓学会誌 ((一社)日本腎臓学会)  56 ( 6 ) 840 - 840 2014.08

Joint Work,  ISSN  0385-2385

HE染色肝病理標本における組織構造定量化によるがん検出精度向上の検討

石川, 雅浩, 藤田, 悠介, Aziz, Maulana Abdul, 村上, 百合, 山口, 雅浩, 小林, 直樹, 喜友名, 朝春, 齋藤, 彰, 阿部, 時也, 橋口, 明典, 坂元, 亨宇

MEDICAL IMAGING TECHNOLOGY 32 ( Suppl. ) 1 - 9 2014.07

Joint Work,  ISSN  0288-450X

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HE染色病理組織標本におけるコンピュータ診断支援の従来研究では、細胞核の形態的特徴を用いる場合が多いが、特に高分化がんあるいは早期がんの検出、診断においては核情報以上に組織構造が重要な役割を持っている。本発表では、肝細胞がんを対象として、これまでに我々が検討を行ってきた組織構造の特徴量をがんの識別に応用した基礎的な結果を報告する。具体的には、肝細胞索の層の厚さ、索構造の乱れ、肝細胞索ごとに算出したN/C比及び核密度などを画像から計測した結果、がんと非がんの間で有意差が認められた。さらに、これらの組織構造の特徴量を従来の細胞核の特徴量に加えることにより、がん識別の精度を向上する可能性が示された。本手法で用いた組織構造の特徴量は、病理組織標本のデジタルスライド画像から自動的に算出できるもので、定量的情報に基づくコンピュータ病理診断支援システムに有用なものと考えられる。(著者抄録)

Effective Color Transform Method for Image Compression of H&E Stained Whole Slide Image Using Subset Pixels

KOBAYASHI Naoki, SHINODA Kazuma, KATOH Ayako, KOMAGATA Hideki, YAMAGUCHI Masahiro, ABE Tokiya, HASHIGUCHI Akinori, SAKAMOTO Michiie

IIEEJ Trans Image Electron Vis Comput (CD-ROM) (The Institute of Image Electronics Engineers of Japan)  2 ( 1 ) 37 - 47 2014.06

Joint Work, Accepted,  ISSN  2188-191X

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抗GBM抗体陽性腎炎発症し、透析導入後にヘノッホ・シェーライン紫斑病(HSP)を併発した一例

小口 英世, 徳山 博文, 脇野 修, 小西 孝之助, 橋口 明典, 林 晃一, 伊藤 裕

日本透析医学会雑誌 ((一社)日本透析医学会)  47 ( Suppl.1 ) 1012 - 1012 2014.05

Joint Work,  ISSN  1340-3451

肥満関連腎症 組織学的変化の新たな知見

徳山 博文, 脇野 修, 橋口 明典, 小西 孝之助, 林 晃一, 伊藤 裕

日本腎臓学会誌 ((一社)日本腎臓学会)  56 ( 3 ) 249 - 249 2014.05

Joint Work,  ISSN  0385-2385

Whole slide imageを用いた膠原・弾性線維定量法

阿部, 時也, 橋口, 明典, 山崎, 剣, 坂元, 亨宇

病理と臨床 32 ( 4 ) 448 - 450 2014.04

Joint Work,  ISSN  0287-3745

Distinct Cell Clusters Touching Islet Cells Induce Islet Cell Replication in Association with Over-Expression of Regenerating Gene (REG) Protein in Fulminant Type 1 Diabetes

Aida, Kaoru, Saitoh, Sei, Nishida, Yoriko, Yokota, Sadanori, Ohno, Shinichi, Mao, Xiayang, Akiyama, Daiichiro, Tanaka, Shoichiro, Awata, Takuya, Shimada, Akira, Oikawa, Youichi, Shimura, Hiroki, Furuya, Fumihiko, Takizawa, Soichi, Ichijo, Masashi, Ichijo, Sayaka, Itakura, Jun, Fujii, Hideki, Hashiguchi, Akinori, Takasawa, Shin, Endo, Toyoshi, Kobayashi, Tetsuro

PLOS ONE (PUBLIC LIBRARY SCIENCE)  9 ( 4 ) 774 - 779 2014.04

Research paper (scientific journal), Joint Work,  ISSN  1932-6203

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Background: Pancreatic islet endocrine cell-supporting architectures, including islet encapsulating basement membranes (BMs), extracellular matrix (ECM), and possible cell clusters, are unclear.
Procedures: The architectures around islet cell clusters, including BMs, ECM, and pancreatic acinar-like cell clusters, were studied in the non-diabetic state and in the inflamed milieu of fulminant type 1 diabetes in humans.
Result: Immunohistochemical and electron microscopy analyses demonstrated that human islet cell clusters and acinar-like cell clusters adhere directly to each other with desmosomal structures and coated-pit-like structures between the two cell clusters. The two cell-clusters are encapsulated by a continuous capsule composed of common BMs/ECM. The acinar-like cell clusters have vesicles containing regenerating (REG) I alpha protein. The vesicles containing REG I alpha protein are directly secreted to islet cells. In the inflamed milieu of fulminant type 1 diabetes, the acinar-like cell clusters over-expressed REG I alpha protein. Islet endocrine cells, including beta-cells and non-beta cells, which were packed with the acinar-like cell clusters, show self-replication with a markedly increased number of Ki67-positive cells.
Conclusion: The acinar-like cell clusters touching islet endocrine cells are distinct, because the cell clusters are packed with pancreatic islet clusters and surrounded by common BMs/ECM. Furthermore, the acinar-like cell clusters express REG I alpha protein and secrete directly to neighboring islet endocrine cells in the non-diabetic state, and the cell clusters over-express REG Ia in the inflamed milieu of fulminant type 1 diabetes with marked self-replication of islet cells.

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Supratentorial neurenteric cyst with spontaneous repetitive intracystic hemorrhage mimicking brain abscess: a case report

Kitamura, Yohei, Sasaki, Hikaru, Hashiguchi, Akinori, Momoshima, Suketaka, Shidoh, Satoka, Yoshida, Kazunari

NEUROSURGICAL REVIEW (SPRINGER)  37 ( 1 ) 153 - 159 2014.01

Research paper (scientific journal), Joint Work,  ISSN  0344-5607

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Neurenteric cyst (NC) is a benign epithelial cyst (BEC) of endodermal origin that mostly occurs in the spinal subdural space or posterior cranial fossa. A 28-year-old male presented with a left frontal lobe NC associated with spontaneous repetitive intracystic hemorrhage, which was initially diagnosed and treated as a brain abscess. He presented with headache and disorientation, without underlying diseases. A cystic tumor was suspected because of a hypointense signal on diffusion-weighted magnetic resonance imaging (MRI). One day after admission, his condition deteriorated rapidly and emergency cyst aspiration was performed. A brown viscous liquid like bloody pus comprising many neutrophils and macrophages was obtained. Although culture was negative, we initially started antibiotic treatment because of cyst content characteristics and rapid clinical course compatible with brain abscess. He was discharged without neurological deficits, but occasionally complained of intense headache. Computed tomography/MRI showed repetitive intracystic hemorrhage and gradual re-enlargement of the lesion. He underwent radical cyst excision by frontal craniotomy 34 months after aspiration. The pathological diagnosis was NC. We believe this is the first report of a supratentorial NC with spontaneous repetitive intracystic hemorrhage. BECs, especially with intracystic hemorrhage, are difficult to be distinguished from brain abscesses. In cases of cystic lesions or presumed brain abscesses refractory to treatment with aspiration and/or antibiotics, BECs should be considered, and radical cyst wall removal should be considered a treatment option.

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Inhibition of Activated Rac1 Signaling Induces Recovery of E-Cadherin Function and Abrogation of Invasive Phenotype of Side Population in Colon Cancer Cells

Funakoshi, Shinsuke, Azuma, Toshifumi, Higuchi, Hajime, Nakano, Masaru, Adachi, Masayuki, Hamamoto, Yasuo, Niibe, Ayano, Hashiguchi, Akinori, Takaishi, Hfromasa, Hibi, Toshifumi

DIGESTION (KARGER)  89 ( 1 ) 83 - 83 2014

Research paper (scientific journal), Joint Work,  ISSN  0012-2823