Presentations -

Structural elucidation of the regulation mechanism of G protein-gated inwardly rectifying potassium channel by G protein heterotrimer

加納花穂, 外山侑樹, 岩橋優太, 間瀬瑶子, 横川真梨子, 大澤匡範, 嶋田一夫

第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会, 

2015.12

, 

Poster presentation

Structural insights into ATP binding and channel gating in bacterial MgtE Mg2+ channels

Atsuhiro Tomita, Hironori Takeda, Tatsuro Maruyama, Masanori Osawa, Ichio Shimada, Ryuichiro Ishitani, Motoyuki Hattori, Osamu Nureki

[Domestic presentation]  日本分子生物学会年会, 

2015.12

, 

Poster presentation

Structural basis for the inhibition of voltage-dependent K+ channel by gating modifier toxin.

OSAWA MASANORI

[Domestic presentation]  VSP研究会, 

2015.11

, 

Oral presentation (general)

NMR method for characterizing microsecond-to-millisecond chemical exchanges utilizing differential multiple-quantum relaxation in high molecular weight proteins

Yuki Toyama, Masanori Osawa, Mariko Yokogawa, Ichio Shimada

[Domestic presentation]  第54回NMR討論会, 

2015.11

, 

Poster presentation, 日本核磁気共鳴学会

Structural insights into ATP binding and channel gating in bacterial MgtE Mg2+ channels

Atsuhiro Tomita, Hironori Takeda, Tatsuro Maruyama, Masanori Osawa, Ichio Shimada, Ryuichiro Ishitani, Motoyuki Hattori, Osamu Nureki

[Domestic presentation]  日本結晶学会年会, 

2015.10

, 

Oral presentation (general)

Structural analyses of the cytosolic region of the chemokine receptor, which interacts with the chemokine signal regulator FROUNT.

Sosuke Yoshinaga, Kaori Esaki, Tatsuichiro Tsuji, Etsuko Toda, Yuya Terashima, Takashi Saitoh, Daisuke Kohda, Toshiyuki Kohno, Masanori Osawa, Takumi Ueda, Ichio Shimada, Kouji Matsushima, Hiroaki Terasawa

[Domestic presentation]  第53回日本生物物理学会年会, 

2015.09

, 

Poster presentation

　View Summary

The membrane-proximal C-terminal region (Pro-C) is important for the regulation of G-protein coupled receptors, but the Pro-C binding to a cytosolic regulator has not been structurally analyzed. The chemokine receptors CCR2/5 bind to the cytosolic regulator FROUNT. The structures of CCR2 Pro-C bound with FROUNT or biomembrane-mimicking micelles were determined by NMR. Cross-saturation-based experiments revealed that the binding surface on Pro-C for FROUNT overlapped with the binding site for membrane, suggesting competitive binding of Pro-C between FROUNT and membrane. These results support an equilibrium model: chemokine binding changes the conformational equilibrium of CCR2/5 toward the active state, and Pro-C switches its binding partner from the membrane to FROUNT.

Structural mechanism underlying the msec-gating of potassium channel

岩橋優太, 外山侑樹, 今井俊輔, 竹内恒, 大澤匡範, 嶋田一夫

[Domestic presentation]  タンパク質科学会年会, 

2015.06

, 

Poster presentation

Structural insights into ATP binding and channel gating in bacterial MgtE Mg2+ channels.

Atsuhiro Tomita, Hironori Takeda, Tatsuro Maruyama, Masanori Osawa, Ichio Shimada, Ryuichiro Ishitani, Motoyuki Hattori, Osamu Nureki.

[International presentation]  COLD SPRING HARBOR ASIA CONFERENCES (Suzhou Dushu Lake Conference Center in Suzhou, China) , 

2015.05

, 

Oral presentation (general)

ポリA上でのPABP多量体化の構造生物学的解析

沢崎綾一, 今井駿輔, 横川真梨子, 臼井遥子, 星野真一, 堅田利明, 嶋田一夫, 大澤匡範

[Domestic presentation]  第136回 日本薬学会年会, 

2015.03

, 

Oral presentation (general), 日本薬学会

メチルTROSY法による膜タンパク質の機能調節メカニズムの解明

OSAWA MASANORI

[Domestic presentation]  第23回ERATOタンパク質関連情報交換会, 

2015.01

, 

Oral presentation (invited, special)