Presentations -

Structural mechanism for the acceleration of voltage-gated proton channel VSOP/Hv1 gating by arachidonic acid

松葉広昭, 横川真梨子, 木村友美, 望月綾野, 川鍋陽, 岡村康司, 嶋田一夫, 大澤匡範

生命科学系学会合同年次大会、第40回日本分子生物学会年会、第90回日本生化学会大会, 

2017.12

, 

Poster presentation

Structure-Free化合物ライブラリー解析システムの構築と検証

池田和由, 清水祐吾, 清水祐吾

第45回 構造活性相関シンポジウム, 

2017.11

, 

Poster presentation

生物活性情報に基づく構造非開示での化合物ライブラリー多様性評価法の開発

清水祐吾, 池田和由, 大澤匡範

第45回 構造活性相関シンポジウム, 

2017.11

, 

Poster presentation

緩和速度の磁場依存性解析によるGタンパク質へのGDP結合の動的制御機構の解明

外山 侑樹, 加納花穂, 間瀬瑤子, 横川真梨子, 大澤匡範, 嶋田一夫

NMR討論会, 

2017.11

, 

Poster presentation

Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses.

Hanaho Kano, Yuki Toyama, Yoko Mase, Mariko Yokogawa, Masanori Osawa, and Ichio Shimada.

2017 Taiwan-Japan Biomedical Symposium on Magnetic Resonance (National Cheng-Kung University, Tainan, Taiwan), 

2017.10

, 

Poster presentation

Development of structure-free design and analysis for compound libraries based on chemical and bioactive diversity.

Yugo Shimizu, Kazuyoshi Ikeda, Kazuyoshi Ikeda.

CBI, 

2017.10

, 

Poster presentation

Paip2による翻訳抑制機構の構造生物学的解析

寒河江 彪流, 横川 真梨子, 沢崎 綾一, 細田 直, 星野 真一, 大澤 匡範

第61回 日本薬学会 関東支部大会, 

2017.09

, 

Poster presentation

B型肝炎ウイルスの感染メカニズムの構造生物学的解析

石場智彬, 横川真梨子, 横田旭美, 室井大輝, 大澤匡範

第61回 日本薬学会 関東支部大会, 

2017.09

, 

Poster presentation

Gating modifier toxinによる電位依存性カリウムイオンチャネル hERG阻害機構の解明

簗瀬 尚美, 松村 一輝, 秋元 まどか, 黒川 洵子, 大澤 匡範

第61回 日本薬学会 関東支部大会, 

2017.09

, 

Poster presentation

ポリA 上のPABP 多量体による翻訳調節メカニズムの解明

沢崎綾一, 今井駿輔, 横川真梨子, 細田直, 星野真一, 三尾宗代, 三尾 和弘, 嶋田一夫, 大澤匡範

第1回　慶應ライフサイエンスシンポジウム, 

2017.08

, 

Poster presentation

Gタンパク質のファミリー選択的なK+チャネル活性制御機構の解明

加納 花穂, 外山 侑樹, 岩橋 優太, 間瀬 瑶子, 横川 真梨子, 大澤 匡範, 嶋田 一夫

第17回日本蛋白質科学会年会 (仙台国際センター（宮城県仙台市）) , 

2017.06

, 

Poster presentation

カリウムチャネルのミリ秒オーダーの開閉の構造機構の解明

岩橋 優太, 外山 侑樹, 今井 駿輔, 大澤 匡範, 嶋田 一夫

第17回日本蛋白質科学会年会 (仙台国際センター（宮城県仙台市）) , 

2017.06

, 

Poster presentation

Structural mechanism of functional regulation of the voltage-gated ion channels by ligand binding to voltage-sensing domain.

大澤匡範

The 94th Annual Meeting of the Physiological Society of Japan (アクトシティ浜松（静岡県浜松市）) , 

2017.03

, 

Oral presentation (invited, special)

Structural mechanism underlying the millisecond gating of K+ channel.

Yuta Iwahashi, Yuki Toyama, Shunsuke Imai, Masanori Osawa, Ichio Shimada.

58th Experimental Nuclear Magnetic Resonance Conference (Asilomar Conference Center, Pacific Grove, California) , 

2017.03

, 

Poster presentation

　View Summary

KcsA is a pH-dependent K+ channel, which opens under acidic conditions. Under the acidic conditions, KcsA switches between open and closed states on a millisecond timescale (msec-gating), which regulates the K+ permeation in cells. However, the structural mechanism of the msec-gating has remained unclear. In this research, we designed KcsA mutants with different open probabilities in the msec-gating, and conducted NMR experiments on these mutants. We revealed that KcsA undergoes an equilibrium between two conformations, which differ in structures of a cytoplasmic transmembrane gate, and the ratios of these conformations well correlate with the open probabilities in the msec-gating. From these results, we conclude that the conformational equilibrium in the cytoplasmic transmembrane gate leads to the msec-gating.

Dynamic regulation of GDP binding to G proteins revealed by magnetic field-dependent NMR relaxation analyses.

Yuki Toyama, Hanaho Kano, Yoko Mase, Mariko Yokogawa, Masanori Osawa, Ichio Shimada.

58th Experimental Nuclear Magnetic Resonance Conference (Asilomar Conference Center, Pacific Grove, California) , 

2017.03

, 

Poster presentation

　View Summary

Heterotrimeric guanine-nucleotide binding proteins (G proteins) serve as molecular switches in signaling pathways, by coupling the activation of cell surface receptors to intracellular responses. Mutations in the G protein alpha subunit (Gα) that accelerate GDP dissociation cause hyperactivation of the downstream effector proteins, leading to oncogenesis. However, the structural mechanism of the accelerated GDP dissociation has remained unclear. Here, we use magnetic field-dependent relaxation analyses to investigate the structural and dynamic properties of Gα. We show that Gα rapidly exchanges between a ground state conformation, which tightly binds to GDP and an excited conformation with reduced GDP affinity. The oncogenic D150N mutation accelerates GDP dissociation by shifting the equilibrium towards the excited conformation.

Molecular mechanism of the ATP-dependent modulation of the Mg2+ channel MgtE for Mg2+ homeositasis.

Atsuhiro Tomita, Mingfeng Zhang, Hironori Takeda, Fei Jin, Tatsuro Maruyama, Masanori Osawa, Ryuichiro Ishitani, Ichio Shimada, Zhiqiang Yan, Motoyuki Hattori, Osamu Nureki.

The 54th Annual Meeting of the Biophysical Society of Japan, 

2016.11

, 

Poster presentation

Developments and applications of multiple quantum NMR methods to characterize functional dynamics of high molecular weight proteins.

Yuki Toyama, Hanaho Kano, Yoko Mase, Mariko Yokogawa, Masanori Osawa, Ichio Shimada.

The 54th Annual Meeting of the Biophysical Society of Japan, 

2016.11

, 

Oral presentation (invited, special)

Structural basis for the inhibition of voltage-dependent K+ channel by gating modifier toxin.

Osawa M.

The 42nd Naito Conference, 

2016.10

, 

Oral presentation (invited, special)

Gating modifier toxinによる電位依存性K+チャネル阻害の構造基盤

大澤匡範

第60回日本薬学会関東支部大会 (東京) , 

2016.09

, 

Oral presentation (invited, special), 日本薬学会関東支部

Structural Basis for the Inhibition of Voltage-dependent K+ Channel by Gating Modifier Toxin.

Osawa M.

The 27th International Conference on Magnetic Resonance in Biological System, 

2016.08

, 

Poster presentation