論文 - 曽我　朋義

キャピラリー電気泳動-飛行時間型質量分析計を用いた大腸がん組織のメタボローム解析

平山明由、紙健次郎、杉本昌弘、冨田勝、江角浩安、曽我朋義

J. Mass Spectrom. Soc. Jpn. 57 （ 3 ） 183 - 186 2009年06月

研究論文（学術雑誌）, 共著

　概要を見る

Capillary electrophoresis-mass spectrometry based metabolomics exhibits unlimited potentials to elucidate global metabolic profiles of various types of samples including clinical tissues. In addition, non-targeted metabolome analysis by this method holds the promise of discovering novel biomarker candidates. In this commentary, we introduce our strategies for studying cancer-specific metabolism by analyzing metabolome of surgically excised tumor tissues.

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CE-MSによるメタボローム解析

曽我 朋義

生物物理化学 52 （ 3 ） 91 - 94 2008年09月

研究論文（学術雑誌）, 単著

　概要を見る

Metabolomics, which can be defined as the measurement of the level of all intracellular metabolites, has become a powerful new tool for gaining insight into cellular and physiological responses. Here we have developed a comprehensive and quantitative analysis method based on capillary electrophoresis mass spectrometry (CE-MS). In this marriage of techniques, CE confers rapid analysis and efficient resolution, and MS provides excellent selectivity and sensitivity. Previous work in our laboratory demonstrated that CE-MS techniques were quite useful for the metabolome analysis. We applied this approach to profile liver metabolites following acetaminophen-induced hepatotoxicity and revealed ophthalmate as a sensitive indicator of reduced glutathione (GSH) depletion. Our results specifically indicate that serum ophthalmate is a sensitive indicator of hepatic GSH depletion, and may be a new biomarker for oxidative stress.

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CE-MSのメタボローム解析への展開

曽我 朋義

J Mass Spectrom Soc Jpn 51 （ 3 ） 407 - 411 2003年06月

研究論文（学術雑誌）, 単著,  ISSN  1340-8097

　概要を見る

Metabolomics, which can be defined as the measurement of the level of all intracellular metabolites, has become a powerful new tool for gaining insight into functional biology. Intercellular metabolites not only provide metabolic phenotypes but also inducers to gene expression. Thus, metabolome analysis will be as important as genome and proteome research. However, very few methods for a large-scale metabolite analysis have been reported. This paper reports a method for the direct and quantitative analysis of charged metabolite using capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS). Its utility is demonstrated in the determination of basal metabolic intermediates of glycolysis and the TCA cycle pathways in Bacillus subtilis cells, yielding new information about how changes in metabolites are related to sporulation events.

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キャピラリー電気泳動法による配合飼料中の8種類の有機酸の同時定量

石黒瑛一, 日比野洋 , 曽我朋義, 柳井博子, 澤田浩和

食品衛生学雑誌 41 （ 4 ） 261 - 267 2000年08月

研究論文（学術雑誌）, 共著,  ISSN  0015-6426

　概要を見る

配合飼料中の8種類の有機酸 (ギ酸, フマル酸, 酒石酸, リンゴ酸, クエン酸, 酢酸, 乳酸及びプロピオン酸) のキャピラリー電気泳動 (CE) による分析法を開発した. 各有機酸は水で同時抽出し, ろ過した後, ろ液を0.02mol/Lの水酸化ナトリウムで希釈し, 限外ろ過フィルターユニット (分画分子量30,000) で除タンパクを行いCEに供した. 3種類の配合飼料に8種類の有機酸を0.05～2.0%の範囲で添加して回収試験を行ったところ, 平均回収率は85.1～107.3%で繰り返し精度 (RSDr) は13.6%以内であった. また, 3試験室において共同試験を行った結果, 平均回収率は87.3～105.0%, 室間再現精度 (RSDR) は16.3%以内であった.

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