Papers - Sato, Kazuki

Point of care ultrasound combined with CTS-6 to diagnose idiopathic carpal tunnel syndrome.

Kimura H, Furuhata R, Matsuo T, Suzuki T, Matsumura N, Sato K, Iwamoto T

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association  2024.01

ISSN  0949-2658

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Antiviral humoral immunity against SARS-CoV-2 omicron subvariants induced by XBB.1.5 monovalent vaccine in infection-naive and XBB-infected individuals.

Kosugi Y, Kaku Y, Hinay AA Jr, Guo Z, Uriu K, Kihara M, Saito F, Uwamino Y, Kuramochi J, Shirakawa K, Takaori-Kondo A, Genotype to Phenotype Japan (G2P-Japan) Consortium, Sato K

The Lancet. Infectious diseases  2024.01

ISSN  1473-3099

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Recent advances in unlinked total elbow arthroplasty in Japan

Iwamoto T., Ikegami H., Tanji A., Kiyota Y., Suzuki T., Matsumura N., Sato K.

JSES International (JSES International)   2024

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Background: Total elbow arthroplasty (TEA) is a valuable therapeutic approach for improving function and relieving pain in severely deformed elbow joints. However, TEA is associated with a high incidence of complications. In Japan, the use of unlinked TEA has a long history, with the development of the Kudo prosthesis marking a significant milestone. Subsequently, various unlinked implant designs have been developed. Although favorable long-term clinical results have been reported, complications remain a concern. To further improve the outcome of unlinked TEA, attempts have been made in recent years to develop various surgical approaches and intraoperative support devices. This review focuses on the clinical outcomes and recent advances in unlinked TEA in Japan. Methods: A comprehensive review of clinical results and advancements in unlinked TEA in Japan was conducted. The analysis included trends in the number of TEA, medium-term and long-term results for unlinked TEA, surgical approaches, or preoperative planning techniques. Results: Several implant designs have been developed in Japan. Clinical studies have reported satisfactory long-term outcomes with these implants, but complications, such as infection, fractures, and dislocation, have been observed. In order to enhance the outcomes of unlinked TEA, various triceps-on approaches have been developed as alternatives to the triceps-detaching approach, which compromises the continuity between the triceps tendon and ulna, leading to inevitable complications related to the triceps tendon. Preservation or repair of the surrounding soft tissues is considered critical for preventing postoperative instability due to the absence of a radial head in the current unlinked TEA design. Computed tomography-based 3-dimensional preoperative planning has been pioneered in Japan, demonstrating its effectiveness in predicting implant size and achieving appropriate implant placement. Additionally, augmented reality–assisted surgery is being explored to accurately translate preoperative planning into the surgical procedure. Conclusion: Unlinked TEA for inflammatory arthritis has exhibited promising long-term results in Japan, with ongoing efforts to improve surgical techniques and preoperative planning. Further advancements are anticipated to prevent complications such as dislocation and peri-implant fractures.

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Prevalence of and Factors Associated with Sports Injuries in 11,000 Japanese Collegiate Athletes.

Kimura T, Mącznik AK, Kinoda A, Yamada Y, Muramoto Y, Katsumata Y, Sato K

Sports (Basel, Switzerland) 12 ( 1 )  2023.12

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Post-Traumatic Pulmonary Hematoma Presenting as Multiple Ring-shaped Spherical Nodules

Ikeda, O; Shimizu, K; Yamada, Y; Sugiura, H; Takahashi, M; Kimura, T; Suzuki, H; Sato, K; Jinzaki, M

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 208 ( 11 ) 1227 - 1230 2023.12

ISSN  1073-449X

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Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models

Takeya H., Itai S., Kimura H., Kurashina Y., Amemiya T., Nagoshi N., Iwamoto T., Sato K., Shibata S., Matsumoto M., Onoe H., Nakamura M.

Scientific Reports (Scientific Reports)  13 ( 1 )  2023.12

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Chitosan has various tissue regeneration effects. This study was designed to investigate the nerve regeneration effect of Schwann cell (SC)-encapsulated chitosan-collagen hydrogel nerve conduit (CCN) transplanted into a rat model of sciatic nerve defect. We prepared a CCN consisting of an outer layer of chitosan hydrogel and an inner layer of collagen hydrogel to encapsulate the intended cells. Rats with a 10-mm sciatic nerve defect were treated with SCs encapsulated in CCN (CCN+), CCN without SCs (CCN−), SC-encapsulated silicone tube (silicone+), and autologous nerve transplanting (auto). Behavioral and histological analyses indicated that motor functional recovery, axonal regrowth, and myelination of the CCN+ group were superior to those of the CCN− and silicone+ groups. Meanwhile, the CCN− and silicone+ groups showed no significant differences in the recovery of motor function and nerve histological restoration. In conclusion, SC-encapsulated CCN has a synergistic effect on peripheral nerve regeneration, especially axonal regrowth and remyelination of host SCs. In the early phase after transplantation, SC-encapsulated CCNs have a positive effect on recovery. Therefore, using SC-encapsulated CCNs may be a promising approach for massive peripheral nerve defects.

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Estimation of maximal lactate steady state using the sweat lactate sensor

Muramoto Y., Nakashima D., Amano T., Harita T., Sugai K., Daigo K., Iwasawa Y., Ichihara G., Okawara H., Sawada T., Kinoda A., Yamada Y., Kimura T., Sato K., Katsumata Y.

Scientific Reports (Scientific Reports)  13 ( 1 )  2023.12

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A simple, non-invasive algorithm for maximal lactate steady state (MLSS) assessment has not been developed. We examined whether MLSS can be estimated from the sweat lactate threshold (sLT) using a novel sweat lactate sensor for healthy adults, with consideration of their exercise habits. Fifteen adults representing diverse fitness levels were recruited. Participants with/without exercise habits were defined as trained/untrained, respectively. Constant-load testing for 30 min at 110%, 115%, 120%, and 125% of sLT intensity was performed to determine MLSS. The tissue oxygenation index (TOI) of the thigh was also monitored. MLSS was not fully estimated from sLT, with 110%, 115%, 120%, and 125% of sLT in one, four, three, and seven participants, respectively. The MLSS based on sLT was higher in the trained group as compared to the untrained group. A total of 80% of trained participants had an MLSS of 120% or higher, while 75% of untrained participants had an MLSS of 115% or lower based on sLT. Furthermore, compared to untrained participants, trained participants continued constant-load exercise even if their TOI decreased below the resting baseline (P < 0.01). MLSS was successfully estimated using sLT, with 120% or more in trained participants and 115% or less in untrained participants. This suggests that trained individuals can continue exercising despite decreases in oxygen saturation in lower extremity skeletal muscles.

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Anaerobic threshold using sweat lactate sensor under hypoxia

Okawara H., Iwasawa Y., Sawada T., Sugai K., Daigo K., Seki Y., Ichihara G., Nakashima D., Sano M., Nakamura M., Sato K., Fukuda K., Katsumata Y.

Scientific Reports (Scientific Reports)  13 ( 1 ) 22865 2023.12

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We aimed to investigate the reliability and validity of sweat lactate threshold (sLT) measurement based on the real-time monitoring of the transition in sweat lactate levels (sLA) under hypoxic exercise. In this cross-sectional study, 20 healthy participants who underwent exercise tests using respiratory gas analysis under hypoxia (fraction of inspired oxygen [FiO2], 15.4 ± 0.8%) in addition to normoxia (FiO2, 20.9%) were included; we simultaneously monitored sLA transition using a wearable lactate sensor. The initial significant elevation in sLA over the baseline was defined as sLT. Under hypoxia, real-time dynamic changes in sLA were successfully visualized, including a rapid, continual rise until volitionary exhaustion and a progressive reduction in the recovery phase. High intra- and inter-evaluator reliability was demonstrated for sLT’s repeat determinations (0.782 [0.607–0.898] and 0.933 [0.841–0.973]) as intraclass correlation coefficients [95% confidence interval]. sLT correlated with ventilatory threshold (VT) (r = 0.70, p < 0.01). A strong agreement was found in the Bland–Altman plot (mean difference/mean average time: − 15.5/550.8 s) under hypoxia. Our wearable device enabled continuous and real-time lactate assessment in sweat under hypoxic conditions in healthy participants with high reliability and validity, providing additional information to detect anaerobic thresholds in hypoxic conditions.

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Adult-onset idiopathic peripheral pulmonary artery stenosis.

Tamura Y, Tamura Y, Shigeta A, Hosokawa K, Taniguchi Y, Inami T, Adachi S, Tsujino I, Nakanishi N, Sato K, Sakamoto J, Tanabe N, Takama N, Nakamura K, Kubota K, Komura N, Kato S, Yamashita J, Takei M, Joho S, Ishii S, Takemura R, Sugimura K, Tatsumi K

The European respiratory journal 62 ( 6 )  2023.12

ISSN  0903-1936

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Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development.

Takada Y, Fierro L, Sato K, Sanada T, Ishii A, Yamamoto T, Kotani T

Science advances 9 ( 47 ) eadg6532 2023.11

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Large magnetocapacitance of up to 456% at room temperature in FeCo/MgAl₂O₄/FeCo(001) magnetic tunnel junctions

Shibata, Y; Sato, K; Sukegawa, H; Kaiju, H

APPLIED PHYSICS EXPRESS 16 ( 11 )  2023.11

ISSN  1882-0778

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Characteristics in patients with adult-onset idiopathic peripheral pulmonary artery stenosis

Tamura, Y; Tamura, Y; Takemura, R; Shigeta, A; Hosokawa, K; Taniguchi, Y; Inami, T; Adachi, S; Tsujita, I; Nakanishi, N; Sato, K; Sakamoto, J; Tanabe, N; Sugimura, K

EUROPEAN HEART JOURNAL 44 2023.11

ISSN  0195-668X

The prevalence and significance of the EIF2AK4 variants in the patients with pulmonary arterial hypertension

Momoi, M; Hiraide, T; Shinya, Y; Sato, K; Inami, T; Fukuda, K; Kataoka, M

EUROPEAN HEART JOURNAL 44 2023.11

ISSN  0195-668X

MRP1-Dependent Extracellular Release of Glutathione Induces Cardiomyocyte Ferroptosis After Ischemia-Reperfusion

Ichihara G., Katsumata Y., Sugiura Y., Matsuoka Y., Maeda R., Endo J., Anzai A., Shirakawa K., Moriyama H., Kitakata H., Hiraide T., Goto S., Ko S., Iwasawa Y., Sugai K., Daigo K., Goto S., Sato K., Yamada K.I., Suematsu M., Ieda M., Sano M.

Circulation Research (Circulation Research)  133 ( 10 ) 861 - 876 2023.10

ISSN  00097330

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BACKGROUND: The membrane components of cardiomyocytes are rich in polyunsaturated fatty acids, which are easily oxidized. Thus, an efficient glutathione-based lipid redox system is essential for maintaining cellular functions. However, the relationship between disruption of the redox system during ischemia-reperfusion (IR), oxidized lipid production, and consequent cell death (ferroptosis) remains unclear. We investigated the mechanisms underlying the disruption of the glutathione-mediated reduction system related to ferroptosis during IR and developed intervention strategies to suppress ferroptosis. METHODS: In vivo fluctuations of both intra- and extracellular metabolite levels during IR were explored via microdialysis and tissue metabolome analysis. Oxidized phosphatidylcholines were assessed using liquid chromatography high-resolution mass spectrometry. The areas at risk following IR were assessed using triphenyl-tetrazolium chloride/Evans blue stain. RESULTS: Metabolomic analysis combined with microdialysis revealed a significant release of glutathione from the ischemic region into extracellular spaces during ischemia and after reperfusion. The release of glutathione into extracellular spaces and a concomitant decrease in intracellular glutathione concentrations were also observed during anoxia-reperfusion in an in vitro cardiomyocyte model. This extracellular glutathione release was prevented by chemical inhibition or genetic suppression of glutathione transporters, mainly MRP1 (multidrug resistance protein 1). Treatment with MRP1 inhibitor reduced the intracellular reactive oxygen species levels and lipid peroxidation, thereby inhibiting cell death. Subsequent in vivo evaluation of endogenously oxidized phospholipids following IR demonstrated the involvement of ferroptosis, as levels of multiple oxidized phosphatidylcholines were significantly elevated in the ischemic region 12 hours after reperfusion. Inhibition of the MRP1 transporter also alleviated intracellular glutathione depletion in vivo and significantly reduced the generation of oxidized phosphatidylcholines. Administration of MRP1 inhibitors significantly attenuated infarct size after IR injury. CONCLUSIONS: Glutathione was released continuously during IR, primarily in an MRP1-dependent manner, and induced ferroptosis. Suppression of glutathione release attenuated ferroptosis and reduced myocardial infarct size following IR.

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Nonobstructive General Angioscopy- Guided Intraventricular Tumor Biopsy Direct Visualization Improved Diagnostic Accuracy and Procedural Safety

Terauchi, T; Sato, K; Watabe, H; Ieda, M

JACC-CARDIOVASCULAR INTERVENTIONS 16 ( 20 ) 2569 - 2571 2023.10

ISSN  1936-8798

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特集 上腕骨骨折の治療戦略 高齢者の上腕骨遠位端骨折

清田 康弘, 佐藤 和毅

関節外科　基礎と臨床 (メジカルビュー社)  42 ( 10 ) 1183 - 1189 2023.10

ISSN  02865394

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Synthesis and characterization of a formal 21-electron cobaltocene derivative

Takebayashi, S; Ariai, J; Gellrich, U; Kartashov, SV; Fayzullin, RR; Kang, HB; Yamane, T; Sugisaki, K; Sato, K

NATURE COMMUNICATIONS 14 ( 1 ) 4979 2023.09

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Association Between the COVID-19 Pandemic and Early Childhood Development

Sato, K; Fukai, T; Fujisawa, KK; Nakamuro, M

JAMA PEDIATRICS 177 ( 9 ) 930 - 938 2023.09

ISSN  2168-6203

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Deep learning-based model detects atrial septal defects from electrocardiography: a cross-sectional multicenter hospital-based study

Miura K., Yagi R., Miyama H., Kimura M., Kanazawa H., Hashimoto M., Kobayashi S., Nakahara S., Ishikawa T., Taguchi I., Sano M., Sato K., Fukuda K., Deo R.C., MacRae C.A., Itabashi Y., Katsumata Y., Goto S.

eClinicalMedicine (eClinicalMedicine)  63 2023.09

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Background: Atrial septal defect (ASD) increases the risk of adverse cardiovascular outcomes. Despite the potential for risk mitigation through minimally invasive percutaneous closure, ASD remains underdiagnosed due to subtle symptoms and examination findings. To bridge this diagnostic gap, we propose a novel screening strategy aimed at early detection and enhanced diagnosis through the implementation of a convolutional neural network (CNN) to identify ASD from 12-lead electrocardiography (ECG). Methods: ECGs were collected from patients with at least one recorded echocardiogram at 3 hospitals from 2 continents (Keio University Hospital from July 2011 to December 2020, Brigham and Women's Hospital from January 2015 to December 2020, and Dokkyo Medical University Saitama Medical Center from January 2010 and December 2021). ECGs from patients with a diagnosis of ASD were labeled as positive cases while the remainder were labeled as negative. ECGs after the closure of ASD were excluded. After randomly splitting the ECGs into 3 datasets (50% derivation, 20% validation, and 30% test) with no patient overlap, a CNN-based model was trained using the derivation datasets from 2 hospitals and was tested on held-out datasets along with an external validation on the 3rd hospital. All eligible ECGs were used for derivation and validation whereas the earliest ECG for each patient was used for the test and external validation. The discrimination of ASD was assessed by the area under the receiver operating characteristic curve (AUROC). Multiple subgroups were examined to identify any heterogeneity. Findings: A total of 671,201 ECGs from 80,947 patients were collected from the 3 institutions. The AUROC for detecting ASD was 0.85–0.90 across the 3 hospitals. The subgroup analysis showed excellent performance across various characteristics Screening simulation using the model greatly increased sensitivity from 80.6% to 93.7% at specificity 33.6% when compared to using overt ECG abnormalities. Interpretation: A CNN-based model using 12-lead ECG successfully identified the presence of ASD with excellent generalizability across institutions from 2 separate continents. Funding: This work was supported by research grants from JST ( JPMJPF2101), JSR corporation, Taiju Life Social Welfare Foundation, Kondou Kinen Medical Foundation, Research fund of Mitsukoshi health and welfare foundation, Tokai University School of Medicine Project Research and Internal Medicine Project Research, Secom Science and Technology Foundation, and Grants from AMED ( JP23hma922012 and JP23ym0126813). This work was partially supported by One Brave Idea, co-funded by the American Heart Association and Verily with significant support from AstraZeneca and pillar support from Quest Diagnostics.

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Influence of Electrodiagnostic Studies on Surgery for Carpal Tunnel Syndrome: A Retrospective Analysis of Data from a Single Urban Institution

Kimura H., Matsuo T., Suzuki T., Matsumura N., Sato K., Iwamoto T.

Journal of Hand Surgery Asian-Pacific Volume (Journal of Hand Surgery Asian-Pacific Volume)  28 ( 4 ) 453 - 460 2023.08

ISSN  24248355

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Background: This study aimed to clarify the influence of preoperative electrodiagnostic (EDX) utilisation and its severity on surgically treated patients with carpal tunnel syndrome (CTS). Methods: This retrospective study analysed the data of patients who underwent CTS surgery at our facility from 2012 to 2022. Data regarding age, sex, affected side, diabetes mellitus, rheumatoid arthritis, haemodialysis and insurance type were collected, along with the following clinical information: utilisation of EDX, steroid injection, number of items of preoperative physical examination and history taking, intervals between symptom onset and the initial visit and between the initial visit and surgery, type of surgical procedure and surgery categorisation (primary or revision). EDX data (EDX severity, interval between EDX and surgery and concomitant nerve lesions) were also investigated. Univariate and multivariate analyses were conducted to assess the influence of EDX utilisation and severity on clinical parameters. Results: Of the 343 hands enrolled in our study, 304 (89%) underwent preoperative EDX assessment. EDX severity was classified as normal, mild, moderate or severe in 6 (2%), 18 (6%), 102 (34%) and 178 hands (58%), respectively. After the univariate and multivariable analyses, more items of preoperative physical examination and history taking were associated with non-EDX utilisation, whereas younger age, female sex and steroid injection were associated with milder EDX. Conclusions: Increased number of items of preoperative physical examination and history taking or steroid injection were independently associated with non-EDX utilisation or milder EDX, respectively, suggesting that when thorough clinical evaluation is conducted, hand surgeons may be less likely to request for EDX before surgery. Moreover, their treatment recommendation may be influenced by EDX severity. Level of Evidence: Level III (Diagnostic)

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