論文 - 竹村　研治郎

Efficient subculture process for adherent cells by selective collection using cultivation substrate vibration

Yuta Kurashina, Kenjiro Takemura, James Friend, Shogo Miyata, Jun Komotori

IEEE Transactions on Biomedical Engineering （IEEE）  64 （ 3 ） 580 - 587 2017年03月

研究論文（学術雑誌）, 共著, 責任著者, 査読有り,  ISSN  0018-9294

　概要を見る

Cell detachment and reseeding are typical operations in cell culturing, often using trypsin exposure and pipetting, even though this process is known to damage the cells. Reducing the number of detachment and reseeding steps might consequently improve the overall quality of the culture, but to date this has not been an option. This study proposes the use of resonant vibration in the cell cultivation substrate to selectively release adherent calf chondrocyte cells: Some were released from the substrate and collected while others were left upon the substrate to grow to confluence as a subculture-without requiring reseeding. An out-of-plane vibration mode with a single nodal circle was used in the custom culture substrate. At a maximum vibration amplitude of 0.6 mu m, 84.9% of the cells adhering to the substrate were released after 3 min exposure, leaving a sufficient number of cells for passage and long-term cell culture, with the greatest cell concentration along the nodal circle where the vibration was relatively quiescent. The 72 h proliferation of the unreleased cells was 20% greater in number than cells handled using the traditional method of trypsin-EDTA (0.050%) release, pipette collection, and reseeding. Due to the vibration, it was possible to reduce the trypsin-EDTA used for selective release to only 0.025%, and in doing so the cell number after 72 h of proliferation was 42% greater in number than the traditional technique.

• Access to Document (DOI)

Cell agglomeration in the wells of a 24-well plate using acoustic streaming

Yuta Kurashina, Kenjiro Takemura, James Friend

Lab on a Chip （Royal Society of Chemistry）  17 （ 5 ） 876 - 886 2017年02月

研究論文（学術雑誌）, 共著, 査読有り,  ISSN  1473-0197

　概要を見る

Cell agglomeration is essential both to the success of drug testing and to the development of tissue engineering. Here, a MHz-order acoustic wave is used to generate acoustic streaming in the wells of a 24-well plate to drive particle and cell agglomeration. Acoustic streaming is known to manipulate particles in microfluidic devices, and even provide concentration in sessile droplets, but concentration of particles or cells in individual wells has never been shown, principally due to the drag present along the periphery of the fluid in such a well. The agglomeration time for a range of particle sizes suggests that shear-induced migration plays an important role in the agglomeration process. Particles with a diameter of 45 mu m agglomerated into a suspended pellet under exposure to 2.134 MHz acoustic waves at 1.5 W in 30 s. Additionally, BT-474 cells also agglomerated as adherent masses at the center bottom of the wells of tissue-culture treated 24-well plates. By switching to low cell binding 24-well plates, the BT-474 cells formed suspended agglomerations that appeared to be spheroids, fully fifteen times larger than any cell agglomerates without the acoustic streaming. In either case, the viability and proliferation of the cells were maintained despite acoustic irradiation and streaming. Intermittent excitation was effective in avoiding temperature excursions, consuming only 75 mW per well on average, presenting a convenient means to form fully three-dimensional cellular masses potentially useful for tissue, cancer, and drug research.

• Access to Document (DOI)

Cell patterning method using resonance vibration of metallic cell cultivation substrate

Chikahiro Imashiro, Yuta Kurashina, Kenjiro Takemura

Advanced Biomedical Engineering 5   142 - 148 2016年12月

研究論文（学術雑誌）, 共著, 最終著者, 責任著者, 査読有り

• Access to Document (DOI)

Coarse Grained Model for Calculating the Ion Mobility of Hydrocarbon

Yuichiro Kuroboshi,Kenjiro Takemura

AIP Advances （AIP Publishing）  6 （ 12 ） 125307 2016年12月

研究論文（学術雑誌）, 共著, 最終著者, 責任著者, 査読有り,  ISSN  2158-3226

　概要を見る

Hydrocarbons are widely used as insulating compounds. However, their fundamental characteristics in conduction phenomena are not completely understood. A great deal of effort is required to determine reasonable ionic behavior from experiments because of their complicated procedures and tight controls of the temperature and the purity of the liquids. In order to understand the conduction phenomena, we have theoretically calculated the ion mobilities of hydrocarbons and investigated their characteristics using the coarse grained model in molecular dynamics simulations. We assumed a molecule of hydrocarbons to be a bead and simulated its dependence on the viscosity, electric field, and temperature. Furthermore, we verified the suitability of the conformation, scale size, and long-range interactions for the ion mobility. The results of the simulations show that the ion mobility values agree reasonably well with the values from Walden's rule and depend on the viscosity but not on the electric field. The ion mobility and self-diffusion coefficient exponentially increase with increasing temperature, while the activation energy decreases with increasing molecular size. These values and characteristics of the ion mobility are in reasonable agreement with experimental results. In the future, we can understand not only the ion mobilies of hydrocarbons in conduction, but also we can predict general phenomena in electrochemistry with molecular dynamics simulations. (C) 2016 Author(s).

• Access to Document (DOI)

Viability of Myoblasts Manipulated by Resonance Vibration of a Disk-shaped Transducer with a Single Nodal Circle

Chikahiro Imashiro, Yuta Kurashina, Misa Nakao, Kenjiro Takemura

EMBS Micro and Nanotechnology in Medicine Conference 2016年12月

研究論文（国際会議プロシーディングス）, 共著, 最終著者, 責任著者, 査読有り

A Method to Generate 3D Cell Tissue using Acoustic Streaming

Yusuke Terao, Chikahiro Imashiro, Yuta Kurashina, Kenjiro Takemura

EMBS Micro and Nanotechnology in Medicine Conference （IEEE）  2016年12月

研究論文（国際会議プロシーディングス）, 共著, 最終著者, 責任著者, 査読有り

Selectively Detaching Highly Proliferative Cells using Temperature Modulation and Resonance Vibration of Cultivation Substrate

Yuta Kurashina, Chikahiro Imashiro, Kenjiro Takemura, Jun Komotori

2016 IEEE International Ultrasonic Symposium Proceedings （IEEE）  2016年09月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り

Manipulation of Myoblasts in Cell Cultivation Dish Using Resonance Vibration of Disk with a Single Nodal Circle

Chikahiro Imashiro, Yuta Kurashina, Kenjiro Takemura

2016 IEEE International Ultrasonic Symposium Proceedings （IEEE）  2016年09月

研究論文（国際会議プロシーディングス）, 共著, 最終著者, 責任著者, 査読有り

Droplet Generating Device for Droplet-based μTAS using Electro-conjugate Fluid

Yoshiki Iijima, Kenjiro Taekmura, Kazuya Edamura

Proceedings of The 15th International Conference on Electrorheological Fluids and Magnetorheological Suspensions    A-42 2016年07月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り

The Experimental and Theoretical Understandings of Electro-conjugate Fluid Flow Generation

Yuichiro Kuroboshi, Kenjiro Takemura

Proceedings of The 15th International Conference on Electrorheological Fluids and Magnetorheological Suspensions    A-41 2016年07月

研究論文（国際会議プロシーディングス）, 共著, 最終著者, 責任著者, 査読有り

Biocompatibility of a droplet-mixing device using an electro-conjugate fluid

Kenjiro Takemura, Kiho Kobayashi, Kazuya Edamura

JSME Mechanical Engineering Journal （Japan Society of Mechanical Engineers）  3 （ 2 ） No.15-00421 2016年02月

研究論文（学術雑誌）, 共著, 筆頭著者, 責任著者, 査読有り

Vision Based Detection of Human Behavior Primitives in Assembly Tasks

Asuka Kobari, Philipp Ennen, Daniel Ewert, Kenjiro Takemura

Proceedings of 2016 7th International Conference on Mechanical, Industrial, and Manufacturing Technologies    55 2016年02月

研究論文（国際会議プロシーディングス）, 共著, 最終著者, 責任著者, 査読有り

Numerical simulation of electro-conjugate fluid flow considering electric double layer

Yoshiki Iijima, Kyohei Hosoda, Kenjiro Takemura, Koji Fukagata, Kazuya Edamura

JSME Mechanical Engineering Journal （Japan Society of Mechanical Engineers）  2 （ 6 ） No. 15-00341 2015年11月

研究論文（学術雑誌）, 共著, 責任著者, 査読有り

• Access to Document (DOI)

A Novel Cell Collection Method using Natural Vibration and Temperature Modulation of Cultivation Base

Yuta Kurashina, Iza Husna Mohamad Hashim, Kenjiro Takemura, Shogo Miyata, Jun Komotori, Tadayoshi Koyama

Proceedings of ASME 2015 International Mechanical Engineering Congress & Exposition （ASME）  2015年11月

研究論文（国際会議プロシーディングス）, 共著, 最終著者, 査読有り

Resonance Vibration and Temperature Modulation Enhances Cell Detachment from Cultivation Substrate

Yuta Kurashina, Iza Husna Mohamad Hashim, Kenjiro Takemura, Shogo Miyata, Jun Komotori, Tadayoshi Koyama

Proceedings of ASME 2015 International Mechanical Engineering Congress & Exposition （ASME）  2015年11月

研究論文（国際会議プロシーディングス）, 共著, 責任著者

Method for Generating Cell Aggregates using Ultrasonic Standing Wave Trapping in a Disposable Capsule

Yuta Kurashina, Kenjiro Takemura, Shogo Miyata, James Friend

2015 IEEE International Ultrasonic Symposium Proceedings （IEEE）     P1B8-4 2015年10月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り

Cell Manipulation by Nodal Circle Resonance Vibration of a Cell Cultivation Substrate

Chikahiro Imashiro, Yuta Kurashina, Kenjiro Takemura, Shogo Miyata, Jun Komotori

2015 IEEE International Ultrasonic Symposium Proceedings （IEEE）     P1B8-5 2015年10月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り,  ISSN  1948-5719

• Access to Document (DOI)

Development of Tactile Evaluation System based on Hierarchical Tactile Perceptive Process

Shogo Kumamoto, Iza Husna Mohamad Hashim, Kenjiro Takemura, Takashi Maeno, Shin Okuda, Yukio Mori

Proc. The 6th International Conference on Manufacturing, Machine Design, and Tribology    376 - 377 2015年04月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り

Numerical Simulation of Electro-conjugation Fluid Flow Considering Electric Double Layer

Yoshiki Iijima, Kyohei Hosoda, Kenjiro Takemura, Shinichi Yokota, Kazuya Edamura

Proc. The 6th International Conference on Manufacturing, Machine Design, and Tribology    466 - 467 2015年04月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り

Cytodetachment Technique using Ultrasonic Vibration

K. Kurashina, K. Takemura, S. Miyara, J. Komotori, T. Koyama

Proc. EMBS Micro and Nanotechnology in Medicine Conference    36 2014年12月

研究論文（国際会議プロシーディングス）, 共著, 責任著者, 査読有り