Hanaoka, Kenjiro

写真a

Affiliation

Faculty of Pharmacy, Department of Pharmaceutical Sciences Analytical Chemistry for Drug Discovery (Shiba-Kyoritsu)

Position

Professor

E-mail Address

E-mail address

Related Websites

External Links

Career 【 Display / hide

  • 2005.04
    -
    2006.03

    日本学術振興会特別研究員(PD)

  • 2005.12
    -
    2007.03

    University of Texas, Southwestern Medical Center, 博士研究員

  • 2006.04
    -
    2007.03

    日本学術振興会特別研究員(SPD)

  • 2007.04
    -
    2010.03

    東京大学, 大学院薬学系研究科, 助教

  • 2010.04
    -
    2011.10

    東京大学, 大学院薬学系研究科, 講師

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Academic Background 【 Display / hide

  • 1996.04
    -
    2000.03

    The University of Tokyo, 薬学部, 薬学科

    Graduated

  • 2000.04
    -
    2002.03

    The University of Tokyo, 大学院薬学系研究科

    Completed, Master's course

  • 2002.04
    -
    2005.03

    The University of Tokyo, 大学院薬学系研究科

    Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(薬学), The University of Tokyo, 2005.03

Licenses and Qualifications 【 Display / hide

  • 薬剤師免許, 2000.07

  • 第一種衛生管理, 2012.02

 

Research Areas 【 Display / hide

  • Bio-related chemistry (Chemical Biology)

  • Chemical biology (Molecular Imaging)

  • Drug development chemistry (Drug DIscovery Chemistry)

Research Themes 【 Display / hide

  • 生命現象の可視化/制御を目指した機能性分子の創製, 

    1999.04
    -
    Present

 

Books 【 Display / hide

  • Development of a small-molecule-based activatable photoacoustic probe

    Ikeno T., Hanaoka K., Urano Y., Methods in Enzymology, 2021.01

     View Summary

    Photoacoustic (PA) imaging is an emerging imaging modality that combines the advantages of optical imaging and ultrasound imaging. In particular, activatable PA probes, which visualize the presence or the activity of target molecules in terms of a change of the PA signal, are useful tools for functional imaging. In this chapter, we describe the development of small-molecule-based activatable PA probes, focusing on the design and synthesis of PA-MMSiNQ, our recently developed activatable PA probe for HOCl. We also describe the protocols used for evaluation of PA-MMSiNQ with a UV–vis spectrometer and a PA imaging microscope.

Papers 【 Display / hide

  • Matrix metalloprotease–14 is a target enzyme for detecting peritoneal metastasis in gastric cancer

    Ogawa S., Kubo H., Murayama Y., Kubota T., Yubakami M., Matsumoto T., Ohashi T., Okamoto K., Kuriki Y., Hanaoka K., Urano Y., Otsuji E.

    Photodiagnosis and Photodynamic Therapy (Photodiagnosis and Photodynamic Therapy)  35 2021.09

    ISSN  15721000

     View Summary

    Background: Accurate diagnosis of peritoneal metastasis in gastric cancer (GC) is important to determine the appropriate treatment. This study aimed to examine whether matrix metalloprotease–14 (MMP–14) was a candidate enzyme in fluorescence imaging for the diagnosis of peritoneal metastasis in GC. Methods: GC and normal peritoneal (NP) tissues from 96 and 20 patients, respectively were evaluated for MMP–14 expression. Live cell imaging of GC cell lines (NUGC4, MKN45, MKN74, HGC-27, and Kato-III) was performed using the MMP–14-activatable fluorescence probe; BODIPY-MMP. Furthermore, the overall survival (OS) was calculated in all patients (n = 96). Results: MMP–14 expression was significantly higher in GC tissues (median: 3.57 ng/mg protein; range:0.64–24.4 ng/mg protein) than in NP tissues (median: 1.34 ng/mg protein; median: 0.53–3.09 ng/mg protein) (P < 0.01). Receiver operating characteristic curves showed that the area under the curve, sensitivity, and specificity were 0.907, 84.4%, and 90.0%, respectively. In live cell imaging using the BODIPY-MMP, fluorescence was observed in five GC cell lines. In the analysis of OS, the high expression of the MMP–14 group had a significantly poorer OS rate than the low expression of the MMP–14 group (P = 0.02). In the multivariate analyses, MMP-14 expression was an independent risk factor for OS (hazard ratio: 2.33; 95 % confidence interval: 1.05–5.45; P = 0.04). Conclusion: MMP–14 is a promising enzyme in intraoperative fluorescence imaging for peritoneal metastasis in GC, especially in patients with poor prognosis.

Research Projects of Competitive Funds, etc. 【 Display / hide

  • Development of inhibiotrs for reactive sulfur species-producing enzymes by utilizing fluorescent probes and their biological analysis

    2020.04
    -
    2022.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 花岡 健二郎, Grant-in-Aid for Scientific Research on Innovative Areas, Principal Investigator

 

Courses Taught 【 Display / hide

  • STUDY OF MAJOR FIELD (ANALYTICAL CHEMISTRY FOR DRUG DISCOVERY)

    2021

  • SEMINAR (ANALYTICAL CHEMISTRY FOR DRUG DISCOVERY)

    2021

  • RESEARCH FOR BACHELOR'S THESIS 1

    2021

  • PHYSICAL CHEMISTRY 2

    2021

  • PHARMACEUTICS LABORATORY COURSE

    2021

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