齋藤 駿 (サイトウ シュン)

SAITO Shun

写真a

所属(所属キャンパス)

理工学部 生命情報学科 (矢上)

職名

助教(有期)

HP

外部リンク

経歴 【 表示 / 非表示

  • 2017年04月
    -
    2019年03月

    東京大学, 農学生命科学研究科 応用生命工学専攻, 特任研究員

  • 2019年04月
    -
    2020年03月

    富山県立大学, 工学研究科 生物・医薬品工学専攻, 嘱託研究員

  • 2020年04月
    -
    継続中

    慶應義塾大学, 理工学部 生命情報学科, 助教(有期)

学歴 【 表示 / 非表示

  • 2008年04月
    -
    2012年03月

    慶應義塾大学, 理工学部, 生命情報学科

    大学, 卒業

  • 2012年04月
    -
    2014年03月

    慶應義塾大学, 理工学研究科 基礎理工学専攻, 生命システム情報専修

    大学院, 修了, 博士前期

  • 2014年04月
    -
    2017年03月

    慶應義塾大学, 理工学研究科 基礎理工学専攻, 生命システム情報専修

    大学院, 修了, 博士後期

学位 【 表示 / 非表示

  • 博士(理学), 慶應義塾大学, 課程, 2017年03月

    微生物由来新規アンドロゲン受容体アンタゴニストAntarlideに関する化学生物学研究

 

研究分野 【 表示 / 非表示

  • ナノテク・材料 / ケミカルバイオロジー

  • ライフサイエンス / 応用微生物学

  • ライフサイエンス / 薬系化学、創薬科学

研究キーワード 【 表示 / 非表示

  • ケミカルバイオロジー

  • 創薬

  • 天然物化学

  • 微生物学

  • 生合成

 

論文 【 表示 / 非表示

  • Identification of 1β,2α-epoxytagitinin C as a Notch inhibitor, oxidative stress mechanism and its anti-leukemia activity

    Makita Y., Saito S., Tsuchiya A., Ishibashi M., Arai M.A.

    Journal of Natural Medicines (Springer Science and Business Media LLC)  76 ( 1 ) 234 - 243 2022年01月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  13403443

     概要を見る

    Notch signaling plays crucial roles in cell differentiation and proliferation, but aberrant activation of this signaling results in tumorigenesis and cancer progression. Notch signaling is thus a promising drug target for oncotherapy, and the development of Notch signaling inhibitors is eagerly awaited. Notch inhibitory activity-guided fractionation of a Spilanthes acmella extract led to the identification of five sesquiterpene lactones: tagitinin A (1), 1β,2α-epoxytagitinin C (2), tagitinin C (3), orizabin (4), and 2α-hydroxytirotundin (5). 1β,2α-Epoxytagitinin C (2) exhibited Notch signaling inhibition, with an IC50 of 25.6 μM, and was further evaluated for its activity against HPB-ALL, a Notch-activated leukemia cell line. Compound 2 showed potent cytotoxicity against HPB-ALL (IC50 1.7 μM) and arrested the cell cycle at the G2/M phase, but did not induce apoptotic cell death. Notch inhibitory mechanism analysis suggested that compound 2 transcriptionally suppresses Notch1 mRNA. In addition, we found that oxidative stress induction is critical for Notch signaling inhibition and the cytotoxicity of compound 2. This is the first mechanism of small molecule Notch inhibition. Our results demonstrate that 1β,2α-epoxytagitinin C (2) is a potential anti-leukemia agent and further investigation of this compound is warranted. Graphical abstract: [Figure not available: see fulltext.].

  • Mycetoindole, an N-acyl dehydrotryptophan with plant growth inhibitory activity from an actinomycete of the genus Actinomycetospora

    Saito S., Oku N., Igarashi Y.

    Journal of Antibiotics (Springer Science and Business Media LLC)  75 ( 1 ) 44 - 47 2022年01月

    研究論文(学術雑誌), 共著, 筆頭著者, 査読有り,  ISSN  00218820

     概要を見る

    A rare actinomycetal strain of the genus Actinomycetospora was found to produce a new tryptophan derivative, designated mycetoindole (1). The structure of 1 was determined to be N-3-methylcrotonoyl (Z)-dehydrotryptophan by NMR and MS analytical methods. Compound 1 reduced the root growth of lettuce Lactuca sativa seedlings at concentrations above 0.1 μM and almost completely inhibited seed germination at 10 μM.

  • Phytohabitols A-C, δ-Lactone-Terminated Polyketides from an Actinomycete of the Genus Phytohabitans

    Saito S., Xiaohanyao Y., Zhou T., Nakajima-Shimada J., Tashiro E., Triningsih D.W., Harunari E., Oku N., Igarashi Y.

    Journal of Natural Products (American Chemical Society (ACS))  2022年

    研究論文(学術雑誌), 共著, 筆頭著者, 査読有り,  ISSN  0163-3864

     概要を見る

    Phytohabitols A−C (1−3), new terminally δ-
    lactonized linear polyketides, were isolated from the culture extract
    of a rare actinomycete of the genus Phytohabitans. The structures
    of 1−3, substituted with multiple methyl and hydroxy groups on a
    conjugated and a skipped diene-containing backbone, were
    elucidated by NMR and MS spectroscopic analyses. The absolute
    configuration of 1 was determined by chemical derivatization and
    chiral anisotropic analysis, coupled with ROESY and J-based
    configuration analysis. In addition, closely similar 1H and 13C
    NMR data and optical rotations among 1−3 supported the same stereochemistry of these polyketides. The related streptomycetes metabolites lagunapyrones B, C, and D have α-pyrone rings on the linear part in place of the δ-lactone, but their chirality at the C19−C21 stereocenters were opposite from those described here, posing a question on the previous assignment made solely by comparison of the optical rotations of four possible diastereomers. Compounds 1−3 inhibited migration of cancer cells with IC50 values of 15, 11, and 8.3 μM, respectively, at noncytotoxic concentrations. In addition, 1−3 displayed potent antitrypanosomal activity against Trypanosoma cruzi with IC50 values of 12, 6.4, and 18 μM, comparable to a commonly used therapeutic drug, benznidazole (IC50 16 μM).

  • Androgen receptor antagonists produced by Streptomyces overcome resistance to enzalutamide

    Imoto M., Fujimaki T., Saito S., Tashiro E.

    Journal of Antibiotics (Springer Science and Business Media LLC)  74 ( 10 ) 706 - 716 2021年10月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  00218820

     概要を見る

    Prostate cancer (PC) is a leading cause of cancer-related death in men in Western countries. Androgen receptor (AR) signaling is a major driver of PC; therefore, androgen deprivation by medical and surgical castration is the standard treatment for patients with PC. However, over time, most patients will progress to metastatic castration-resistant PC. Enzalutamide is the only AR antagonist approved by the Food and Drug Administration for the treatment of metastatic castration-resistant PC. However, resistance to enzalutamide also develops in most patients with castration-resistant PC. Thus, there is an urgent need to develop new AR antagonists with new structures. For this purpose, we conducted both in silico and natural product screenings. From the in silico screening, we obtained T5853872 and more potent compound, STK765173. From the natural product screening, the novel compound arabilin was isolated from Streptomyces sp. MK756-CF1. Unlike STK765173, arabilin could overcome resistance to enzalutamide. Furthermore, we also extracted a novel compound, antarlide A, and its geometric isomers from Streptomyces sp. BB47. Antarlides A–F have novel 22-membered-ring macrocyclic structures, while antarlides G and H have 20-membered-ring structures. Both antarlides B and G showed potent AR antagonist activity in prostate cancer cells and could overcome resistance to enzalutamide.

  • Ultrahigh Thermoresistant Lightweight Bioplastics Developed from Fermentation Products of Cellulosic Feedstock

    Nag A., Ali M.A., Kawaguchi H., Saito S., Kawasaki Y., Miyazaki S., Kawamoto H., Adi D.T.N., Yoshihara K., Masuo S., Katsuyama Y., Kondo A., Ogino C., Takaya N., Kaneko T., Ohnishi Y.

    Advanced Sustainable Systems (Wiley)  5 ( 1 )  2021年01月

    研究論文(学術雑誌), 共著, 査読有り,  ISSN  2366-7486

     概要を見る

    Production of bioplastics from renewable biological resources is a prerequisite for the development of a circular and sustainable society. Current bioplastics are mostly heat-sensitive aliphatic polymers, requiring thermoresistant aromatic bioplastics. Herein, 3-amino-4-hydroxybenzoic acid (AHBA) and 4-aminobenzoic acid (ABA) are produced from kraft pulp, an inedible cellulosic feedstock, using metabolically engineered bacteria. AHBA is chemically converted to 3,4-diaminobenzoic acid (DABA); subsequently, poly(2,5-benzimidazole) is obtained by the polycondensation of DABA and processed into an ultrahigh thermoresistant film. The copolymerization of DABA with a small amount of ABA dramatically increases the degradation temperatures of the resulting films (over 740 °C) to yield the most thermoresistant plastic on record. Density functional theory calculations indicate that the incorporation of ABA strengthens the interchain hydrogen bonds between aromatic imidazole rings. Thus, an alternative organic molecular design is proposed for thermoresistant plastics without using heavy inorganics, although continuous aromatic heterocycles are widely considered ideal for polymer thermoresistance. This innovative macromolecular design increases thermoresistance and can be widely applied to well-processable plastics for the production of lightweight materials and is expected to contribute to the development of a more sustainable society.

全件表示 >>

総説・解説等 【 表示 / 非表示

  • 天然物ケミカルバイオロジー研究の現状 –前立腺がん治療薬を例として–

    齋藤 駿, 井本 正哉

    月刊「ケミカルエンジニヤリング」 (化学工業社)  62   52 - 58 2017年10月

    共著, 筆頭著者

  • 新奇天然化合物ハンティング

    齋藤 駿, 井本 正哉

    月刊化学 (株式会社 化学同人)  71   68 - 69 2016年07月

    共著, 筆頭著者

  • 創薬を志向した天然化合物の探索研究

    齋藤 駿, 田代 悦, 井本 正哉

    化学と生物 (公益社団法人 日本農芸化学会)  52   466 - 472 2014年07月

    共著, 筆頭著者

競争的研究費の研究課題 【 表示 / 非表示

  • 未研究希少放線菌の二次代謝能の解明を通じた新規植物生長制御物質の探索

    2020年04月
    -
    2023年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 齋藤 駿, 若手研究, 補助金,  研究代表者

受賞 【 表示 / 非表示

  • 藤原賞

    2017年03月, 慶應義塾大学 理工学部

    受賞区分: 塾内表彰等

  • 第8回日韓ケミカルバイオロジーシンポジウム Best poster award

    2016年01月

    受賞区分: 国際学会・会議・シンポジウム等の賞

 

担当授業科目 【 表示 / 非表示

  • 理工学基礎実験

    2022年度

  • 基礎生命実験

    2022年度

  • 生命情報実験B

    2022年度

  • 生命情報実験A

    2022年度

  • 理工学基礎実験

    2021年度

全件表示 >>

担当経験のある授業科目 【 表示 / 非表示

  • 生命情報特別講義第2

    慶應義塾大学

    2020年04月
    -
    継続中

    春学期, 講義