SAITO Shun

写真a

Affiliation

Faculty of Science and Technology, Department of Biosciences and Informatics （Yagami）

Position

Senior Assistant Professor (Non-tenured)/Assistant Professor (Non-tenured)

Related Websites

External Links
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Career 【 Display / hide 】

  • 2017.04
    -
    2019.03

    The University of Tokyo, Department of Biotechnology, Graduate School of Agricultural and Life Sciences, Research Associate

  • 2019.04
    -
    2020.03

    Toyama Prefectural University, Biotechnology Research Center and Department of Biotechnology, Research Associate

  • 2020.04
    -
    2023.03

    Keio University, Faculty of Science and Technology Department of Biosciences and Infomatics, Assistant Professor

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Academic Background 【 Display / hide 】

  • 2008.04
    -
    2012.03

    Keio University, 理工学部, 生命情報学科

    University, Graduated

  • 2012.04
    -
    2014.03

    Keio University, 理工学研究科 基礎理工学専攻, 生命システム情報専修

    Graduate School, Completed, Master's course

  • 2014.04
    -
    2017.03

    Keio University, 理工学研究科 基礎理工学専攻, 生命システム情報専修

    Graduate School, Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

  • Doctor (Science), Keio University, Coursework, 2017.03

    Chemistry and biology of novel androgen receptor antagonist antarlides from microbial origin

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Chemical biology

  • Life Science / Applied microbiology

  • Life Science / Pharmaceutical chemistry and drug development sciences

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Research Keywords 【 Display / hide 】

  • Chemical Biology

  • Drug Discovery

  • Natural Product Chemistry

  • Microbiology

  • Biosynthesis

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Papers 【 Display / hide 】

  • 3-Hydroxy-3-(2-oxopropyl)indolin-2-one, a product of a human-derived Enterocloster strain, is an inhibitor of nitric oxide production

    Saito S., Banno T., Arai M.A.

    Bioscience, Biotechnology and Biochemistry （Bioscience, Biotechnology and Biochemistry)  88 （ 3 ） 316 - 321 2024.03

    ISSN  09168451

    　View Summary

    When cultured anaerobically, Enterocloster sp. RD014215 was found to produce 1 . Using nuclear magnetic resonance and mass spec- troscopy, the planar structure of 1 was determined to be 3-hydroxy-3-(2-oxopropyl)indolin-2-one. The chirality of 1 was implied as S by comparing the optical rotation value of 1 with literature reports of the synthesized compounds. To our knowledge, this work represents the first discovery of the metabolite produced by Enterocloster strain. 1 exhibited inhibition of nitric oxide (NO) production, demonstrating a 50% inhibitory activity (IC50 ) of 34 μm for NO production by murine macrophage cells subjected to lipopolysaccharide stimulation.

  • Notch activator cyclopiazonic acid induces apoptosis in HL-60 cells through calcineurin activation

    Suzuki S., Saito S., Narushima Y., Kodani S., Kagaya N., Suenaga H., Shin-ya K., Arai M.A.

    Journal of Antibiotics （Journal of Antibiotics)  77 （ 1 ） 30 - 38 2024.01

    ISSN  00218820

    　View Summary

    We screened a library of microbial extracts and compounds library using our constructed assay cells and found pulicatins F (1) and G (2), and cyclopiazonic acid (CPA) (3) as Notch activators. Pulicatin F (1) and (±)-pulicatin G were synthesized and their activities were evaluated. Notch activation of CPA (3) was investigated using Western blot and RT-PCR. CPA (3) increased protein level of HES1 and mRNA expression of HES1. Also, the expression of FMS-like tyrosine kinase 3 (FLT3), which was known to inhibit apoptosis, was also inhibited by CPA (3) addition. The Notch activation by CPA (3) and cytotoxicity against HL-60 were clearly canceled by addition of FK506, which is an inhibitor of calcineurin (CaN). In addition, it was revealed that CPA (3) induced apoptosis in HL-60 cells.

  • Methodology for awakening the potential secondary metabolic capacity in actinomycetes

    Saito S., Arai M.A.

    Beilstein Journal of Organic Chemistry （Beilstein Journal of Organic Chemistry)   （ 20 ） 753 - 766 2024

    　View Summary

    Secondary metabolites produced by actinomycete strains undoubtedly have great potential for use in applied research areas such as drug discovery. However, it is becoming difficult to obtain novel compounds because of repeated isolation around the world. Therefore, a new strategy for discovering novel secondary metabolites is needed. Many researchers believe that actinomycetes have as yet unanalyzed secondary metabolic activities, and the associated undiscovered secondary metabolite biosynthesis genes are called “silent” genes. This review outlines several approaches to further activate the metabolic potential of actinomycetes.

  • Dihydromaniwamycin E, a Heat-Shock Metabolite from Thermotolerant Streptomyces sp. JA74, Exhibiting Antiviral Activity against Influenza and SARS-CoV-2 Viruses

    Saito S., Funayama K., Kato W., Okuda M., Kawamoto M., Matsubara T., Sato T., Sato A., Otsuguro S., Sasaki M., Orba Y., Sawa H., Maenaka K., Shindo K., Imoto M., Arai M.A.

    Journal of Natural Products （Journal of Natural Products)  85 （ 11 ） 2583 - 2591 2022.11

    ISSN  01633864

    　View Summary

    Dihydromaniwamycin E (1), a new maniwamycin derivative featuring an azoxy moiety, has been isolated from the culture extract of thermotolerant Streptomyces sp. JA74 along with the known analogue maniwamycin E (2). Compound 1 is produced only by cultivation of strain JA74 at 45 °C, and this type of compound has been previously designated a "heat shock metabolite (HSM)" by our research group. Compound 2 is detected as a production-enhanced metabolite at high temperature. Structures of 1 and 2 are elucidated by NMR and MS spectroscopic analyses. The absolute structure of 1 is determined after the total synthesis of four stereoisomers. Though the absolute structure of 2 has been proposed to be the same as the structure of maniwamycin D, the NMR and the optical rotation value of 2 are in agreement with those of maniwamycin E. Therefore, this study proposes a structural revision of maniwamycins D and E. Compounds 1 and 2 show inhibitory activity against the influenza (H1N1) virus infection of MDCK cells, demonstrating IC50values of 25.7 and 63.2 μM, respectively. Notably, 1 and 2 display antiviral activity against SARS-CoV-2, the causative agent of COVID-19, when used to infect 293TA and VeroE6T cells, with 1 and 2 showing IC50values (for infection of 293TA cells) of 19.7 and 9.7 μM, respectively. The two compounds do not exhibit cytotoxicity in these cell lines at those IC50concentrations.

  • Noaoxazole, a new heat shock metabolite produced by thermotolerant Streptomyces sp. HR41

    Saito S., Suzuki S., Arai M.A.

    Journal of Antibiotics （Journal of Antibiotics)  75 （ 9 ） 509 - 513 2022.09

    ISSN  00218820

    　View Summary

    The thermotolerant strain Streptomyces sp. HR41 was found to produce compound 1 only in a 45 °C culture, and not at the standard temperature. We previously designated this type of compound as a “heat shock metabolite” (HSM). NMR and MS analytical techniques were used to determine that the chemical structure of 1 comprised a methylated-oxazole ring and a linear chain moiety modified with a terminal amide group. Thus, 1 was shown to be a new curromycin analog, which we have designated noaoxazole (1). Compound 1 weakly activated Notch signal reporter activity without exhibiting cytotoxicity against assay cells at the same concentration.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 天然物ケミカルバイオロジー研究の現状 –前立腺がん治療薬を例として–

    齋藤　駿, 井本　正哉

    月刊「ケミカルエンジニヤリング」 (化学工業社)  62   52 - 58 2017.10

    Joint Work, Lead author

  • 新奇天然化合物ハンティング

    齋藤　駿, 井本　正哉

    月刊化学 (株式会社 化学同人)  71   68 - 69 2016.07

    Joint Work, Lead author

  • 創薬を志向した天然化合物の探索研究

    齋藤　駿, 田代　悦, 井本　正哉

    化学と生物 (公益社団法人 日本農芸化学会)  52   466 - 472 2014.07

    Joint Work, Lead author

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 放線菌が生産する熱ショック代謝物（HSM）の生産制御および耐熱性促進機構の解析

    2023.04
    -
    2026.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists , Principal investigator

  • 放線菌が生産する熱ショック代謝物 (HSM) の耐熱性促進機構の解析

    2023.04
    -
    2024.03

    公益財団法人 野田産業科学研究所, 奨励研究助成, Principal investigator

  • 未研究希少放線菌の二次代謝能の解明を通じた新規植物生長制御物質の探索

    2020.04
    -
    2023.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists , Principal investigator

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Awards 【 Display / hide 】

  • 令和5年度生薬天然物部会奨励研究

    2023.10, 日本薬学会 生薬天然物部会

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • 2021 JA Ōmura Awards

    2022.12, The Journal of Antibiotics

    Type of Award： Honored in official journal of a scientific society, scientific journal

  • 第32回新薬創製談話会 研究奨励賞

    2022.09

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • 藤原賞

    2017.03, 慶應義塾大学 理工学部

    Type of Award： Keio commendation etc.

  • 第8回日韓ケミカルバイオロジーシンポジウム Best poster award

    2016.01

    Type of Award： Award from international society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • TOPICS IN BIOSCIENCES AND INFORMATICS 2

    2024

  • SEMINAR IN BIOSCIENCES AND INFORMATICS

    2024

  • LABORATORIES IN SCIENCE AND TECHNOLOGY

    2024

  • INTRODUCTION TO BIOLOGY TODAY

    2024

  • GLOBAL LEADERSHIP SEMINAR

    2024

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Courses Previously Taught 【 Display / hide 】

  • 生命情報特別講義第2

    慶應義塾大学

    2020.04
    -
    Present
    , 

    Spring Semester, Lecture

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