Kazoe, Yutaka



Faculty of Science and Technology, Department of System Design Engineering (Yagami)


Associate Professor


Books 【 Display / hide

  • マイクロ・ナノ熱工学の進展, 第4章 マイクロチャネル(1)流れの特性

    嘉副裕, エヌ・ティー・エス, 2021.05

  • Extended-Nano Scale Fluidics and Applications to Bioanalysis, in Intelligent Nanosystems for Energy, Information and Biological Technologies

    Hisashi Shimizu, Kazuma Mawatari, Yutaka Kazoe, Yuriy Pihosh, Takehiko Kitamori, Springer, 2016

    Scope: pp. 65-84

  • Micro and extended-nano fluidics and optics for chemical and bioanalytical technology, in “Progress in Nanophotonics 2” Ed. Motoichi Ohtsu

    Kazuma Mawatari, Yuriy Pihosh, Hisashi Shimizu, Yutaka Kazoe, Takehiko Kitamori, Springer-Verlag Berlin Heidelberg, 2013

  • Extended-nano fluidic systems for chemistry and biotechnology

    Kazuma Mawatari, Takehiko Tsukahara, Yo Tanaka, Yutaka Kazoe, Philip Dextras, Takehiko Kitamori, Imperial College Press, 2011

Papers 【 Display / hide

  • Motion of submicrometer particles in micrometer-size channel measured by defocusing nano-particle image velocimetry

    Yuki Kuwano, Minori Tanaka, Yutaka Kazoe

    JOURNAL OF APPLIED PHYSICS (AIP Publishing)  131 ( 10 )  2022.03

    Accepted,  ISSN  00218979

     View Summary

    Understanding the motion of colloidal particles flowing in small spaces is a general issue in various fields such as thermal engineering and micro/nanofluidics. In the present study, we investigated the motion of fluorescent submicrometer particles in a 3-mu m microchannel by defocusing nanoparticle image velocimetry. An optical measurement system with controlled spherical aberration and an algorithm for processing defocused particle images with multiple diffraction rings were developed. By detecting the centroid position and the diameter of the outermost diffraction ring, which is proportional to the distance between the focal plane and the particle, the position of particles was determined with the spatial resolutions of 154-204 nm in the streamwise direction and 76-311 nm in the depthwise direction, which are comparable to or smaller than the optical diffraction limit. A reusable microfluidic device containing a size-regulated microchannel made of glass was developed, which is suitable for optical measurements and precise flow control. By controlling the strength of low-temperature glass bonding, detachment of the bonded glass substrates, washing, and reuse were achieved. Based on this method and technology, the velocity of particles with diameters of 199, 457, and 1114 nm was successfully measured in pressure-driven laminar flow. Results suggested that for larger particles comparable to the channel size, the particle velocity is slowed from the flow velocity by particle-wall hydrodynamic interactions. Therefore, the motion of colloidal particles in 10(0)-mu m spaces is considered to be affected by particle-wall hydrodynamic interactions, as well as 10(2)-mu m spaces reported previously.

  • Characterization of Pressure-Driven Water Flows in Nanofluidic Channels by Mass Flowmetry

    Yutaka KAZOE, Sho KUBORI, Kyojiro MORIKAWA, Kazuma MAWATARI, Takehiko KITAMORI

    Analytical Sciences (Springer Science and Business Media LLC)  38   281 - 287 2022.03

    Accepted,  ISSN  0910-6340

  • Characterization of pressure-driven water flows in nanofluidic channels by mass flowmetry

    Kazoe Y., Kubori S., Morikawa K., Mawatari K., Kitamori T.

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (Analytical sciences : the international journal of the Japan Society for Analytical Chemistry)  38 ( 2 ) 281 - 287 2022.02

     View Summary

    With developments in analytical devices promoted by nanofluidics, estimation of the flow rate in a nanochannel has become important to calculate volumes of samples and reagents in chemical processing. However, measurement of the flow rate in nanospaces remains challenging. In the present study, a mass flowmetry system was developed, and the flow rate of water by pressure-driven flow in a fused-silica nanochannel was successfully measured in picoliters per second. We revealed that the water flow rate is dependent on the viscosity significantly increased in a square nanochannel with 102 nm width and depth (3.6 times higher than the bulk viscosity for a representative channel size of 190 nm) and slightly increased in a plate nanochannel with micrometer-scale width and 102 nm depth (1.3 times higher for that of 234 nm), because of dominant surface effects. The developed method and results obtained will greatly contribute to nanofluidics and other related fields.

  • Characterization of pressure-driven water flows in nanofluidic channels by mass flowmetry.

    Kazoe Y, Kubori S, Morikawa K, Mawatari K, Kitamori T

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 38 ( 2 ) 281 - 287 2022.02

    ISSN  0910-6340

  • Picoliter liquid handling at gas/liquid interface by surface and geometry control in a micro-nanofluidic device

    Kyojiro Morikawa, Shin-ichi Murata, Yutaka Kazoe, Kazuma Mawatari, Takehiko Kitamori


    Accepted,  ISSN  09601317

     View Summary

    In micro- and nanofluidic devices, highly precise fluidic control is essential. Conventional mechanical valves in microchannels and nanochannels have size limitations, whereas hydrophobic (Laplace) valves are generally difficult to use for low-surface-tension liquids. In the present study, we developed a method for handling picoliter volumes of low-surface-tension liquids in a micro-nanofluidic device. The proposed Laplace valve is based on the pinning effect. A fused silica micro-nanofluidic device that includes a picoliter chamber whose geometry was designed to induce capillary pinning was designed and fabricated. The measured Laplace pressure of a lysis buffer (surfactant) was consistent with the calculated pressure, indicating successful fabrication and hydrophobic surface modification. The working principle of the Laplace valve was verified. The Laplace valve maintained the lysis buffer at the gas/liquid interface for 60 min, which is sufficiently long for cell lysis operations. Finally, replacement of liquids in the picoliter chamber using the valve was demonstrated. The proposed method will contribute to basic technologies for fluidic control in micro- and nanofluidic devices, and the proposed Laplace valve can be used for low-surface-tension liquids. In addition, the developed valve and picoliter chamber can be utilized for the interface in single-cell lysis, which will facilitate the development of single-cell analysis devices.

display all >>

Papers, etc., Registered in KOARA 【 Display / hide

Reviews, Commentaries, etc. 【 Display / hide

Presentations 【 Display / hide

  • Femtoliter-droplet shooter by gas/liquid nanofluidics for an interface of mass spectrometry

    Yutaka Kazoe

    The 17th IEEE International Conference on Nano/Micro Engineered & Molecular Systems (IEEE-NEMS 2022), 


  • ナノ流体工学による超高感度1分子分析技術の開発


    第5回Skin Disease Research Conference, 


  • 部分疎水修飾マイクロ流路での油水平行二相流を用いた脂質二重膜合成法の開発

    竹添直之, 嘉副裕



  • 1粒子輸送に向けたナノ流路における微小液滴生成法の開発

    大穂亮介, 嘉副裕



  • 部分疎水修飾マイクロ流路での油水平行二相流操作による脂質二重膜合成法の開発

    竹添直之, 嘉副裕



display all >>

Research Projects of Competitive Funds, etc. 【 Display / hide

  • Development of super-resolution three-dimensional non-fluorescent single particle tracking method for nanospaces utilizing light scattering


    Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A), No Setting

     View Summary

    10-100 nmのナノ空間の流体科学・工学の進展に伴い、表面支配の極小空間における溶質分子輸送現象の解明が重要となっている。本研究では、独自の技術と方法論に基づき光散乱を用いたナノ空間非蛍光ナノ粒子の超解像度追跡法を創成することを目的とする。サイズと表面粗さを1 nm以下の精度で制御したガラス製ナノ流路を作製して、バックグラウンド光を極限まで低減して光学的に整えた条件下で、ナノ粒子の散乱光と流路壁面からの反射光との干渉により形成される粒子像を高速・高感度に検出し、位相差に伴う粒子像変化からナノ空間を流れる非蛍光ナノ粒子の位置をx, y, zの3方向で10 nm分解能で測定する。

  • Development of a method for single-molecule chemical processing utilizing ultra-small droplets in nanospace and application to cytokine analysis


    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Pioneering), Principal investigator

     View Summary


  • Development of a fabrication method for cellular membrane-integrated nanofluidic device utilizing nanochannel parallel two-phase flows


    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory) , Principal investigator

     View Summary


  • Development of a 3D3C super-resolution measurement method for flow velocity distribution in nanospace utilizing defocusing particle image with light interference


    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

     View Summary

    流体工学が光の波長より小さいナノ空間へと進展している。しかし、空間が幅・深さ数100 nm以下になると液体の物性変化など様々な特異的現象が発現するため、この空間の物質輸送を明らかにする超解像度(10 nm分解能)の流速分布計測が重要となる。流速分布計測法としては粒子画像流速計(PIV)が一般的であるが、(x, y, z)3方向に対して超解像度を実現することは極めて難しかった。そこで本研究では、レンズの結像で問題となる球面収差を積極活用・制御して、非焦点領域の粒子像からナノ粒子の位置を10 nm分解能で検出するデフォーカス・ナノPIVを開発する。

  • Creation of Extended-Nano Thermo-Optical Fluidic Device and Realization of Nonlabeled Single Molecule Detection


    The University of Tokyo, Grants-in-Aid for Scientific Research, Kitamori Takehiko, Grant-in-Aid for Scientific Research (A), No Setting

     View Summary

    Analytical methods utilizing small spaces, as represented by micro and extended-nano fluidic devices, have been developed. We have developed thermal lensing microscope (TLM) and photothermal optical phase shift (POPS) detection to detect non-fluorescent molecules in small spaces with high sensitivity. However, the UV-excitation POPS detection has some problems such as heat dissipation in the extended-nano channel and insufficient reduction of optical background due to interference, which hinders measurements with high sensitivity as TLM. In this study, we developed an extended-nano fluidic device with an integrated thermo-optic layer to recover the sensitivity loss due to the thermal diffusion and a background-free POPS detector to achieve ultrahigh sensitivity.

display all >>


Courses Taught 【 Display / hide











display all >>