Moriwaki, Yasuhiro

写真a

Affiliation

Faculty of Pharmacy, Department of Pharmacy 薬学教育研究センター ( Shiba-Kyoritsu )

Position

Assistant Professor/Senior Assistant Professor

Career 【 Display / hide 】

  • 2002.04
    -
    2003.03

    大阪府立成人病センター研究所, 日本学術振興会特別研究員

  • 2003.04
    -
    2003.08

    理化学研究所　脳科学総合研究センター, 日本学術振興会特別研究員

  • 2003.09
    -
    2005.03

    理化学研究所　脳科学総合研究センター, 研究員

  • 2005.04
    -
    2007.03

    共立薬科大学, 助手

  • 2007.04
    -
    2008.03

    共立薬科大学, 助教

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Academic Background 【 Display / hide 】

  • 2000.04
    -
    2003.03

    Nara Institute of Science and Technology, Graduate School, Division of Biological Science

    Graduate School, Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

  • 博士（バイオサイエンス）, 奈良先端科学技術大学院大学, Coursework, 2003.03

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Licenses and Qualifications 【 Display / hide 】

  • 第一種放射線取扱主任者（選任）, 2022.04

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Papers 【 Display / hide 】

  • Immune Niche Formation in Engineered Mouse Models Reveals Mechanisms of Tumor Dormancy

    Ahad A, Leng F, Ichise H, Schrom E, So JY, Sellner C, Gu Y, Wang W, Kieu C, Park WY, Yang R, Wolcott K, Livak F, Kruhlak M, Aprelikova O, Gray J, Kopardé VN, Moriwaki Y, Germain RN, Yang L.

    bioRxiv.    2025.04.16.649000. 2025.04

    Research paper (scientific journal), Joint Work

  • IgG-Binding Peptidomimetic Mixed-Charge Polymer-Modified Resins for Chromatographic Purification of Antibodies.

    Deura K, Sakama A, Moriwaki Y, Citterio D, Hiruta Y.

    ACS Appl Mater Interfaces. (American Chemical Society (United States))   2024.11

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  19448244

    　View Summary

    The process of antibody purification using Fc affinity ligands such as protein A, G, and L faces several challenges including high cost, low stability, and loss of antibody activity due to harsh elution conditions. Here, we describe a chromatographic purification of antibodies utilizing a pH-responsive mixed-charge polymer that mimics the IgG-binding peptide (Z34C) derived from the B domain of protein A. The protein A mimetic resins were prepared by modifying the surface of a TOYOPEARL, methacrylate resin with a polymer that mimics the amino acid sequence of Z34C and the functions of histidine and acidic and neutral amino acids using histamine methacrylamide (HisMA), methacrylic acid, and neutral monomers. The therapeutic monoclonal antibody (mAb), rituximab, was retained on the column at pH 7 and eluted under mildly acidic conditions at pH 5 using a protein A mimetic resin (HisMA20-EEMA) optimized for antibody interaction. The injected antibodies were selectively captured on the column by hydrophobic and electrostatic interactions with the protein A mimetic polymer under neutral conditions and eluted by electrostatic repulsion under acidic conditions. The HisMA20-EEMA column successfully purified mAbs from mixtures with BSA, mouse ascites fluid, and hybridoma cell culture supernatant. In addition, the HisMA20-EEMA column consistently achieved 90% antibody recovery in 100 consecutive purifications from cell culture supernatant. The antibody purification method presented in this study is low cost, highly durable, easy to synthesize, and allows for mild elution conditions. The results demonstrate that the approach of mimicking IgG-binding peptides with mixed-charge polymers is useful for the development of column packing materials for antibody purification.

  • Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia

    Murata T, Tago K, Miyata K, Moriwaki Y, Misawa H, Kobata K, Nakazawa Y, Tamura H, Funakoshi-Tago M.

    International Journal of Molecular Sciences (International Journal of Molecular Sciences)  25 ( 1 )  2023.12

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  16616596

    　View Summary

    According to numerous studies, it has been epidemiologically suggested that habitual coffee intake seems to prevent the onset of neurodegenerative diseases. In this study, we hypothesized that coffee consumption suppresses neuroinflammation, which is closely related to the development of neurodegenerative diseases. Using microglial BV-2 cells, we first found that the inflammatory responses induced by lipopolysaccharide (LPS) stimulation was diminished by both coffee and decaffeinated coffee through the inhibition of an inflammation-related transcription factor, nuclear factor-κB (NF-κB). Pyrocatechol, a component of roasted coffee produced by the thermal decomposition of chlorogenic acid, also exhibited anti-inflammatory activity by inhibiting the LPS-induced activation of NF-κB. Finally, in an inflammation model using mice injected with LPS into the cerebrum, we observed that intake of pyrocatechol as well as coffee decoctions drastically suppressed the accumulation of microglia and the expression of interleukin-6 (IL-6), tumor necrosis factor α (TNFα), CCL2, and CXCL1 in the inflammatory brain. These observations strongly encourage us to hypothesize that the anti-inflammatory activity of pyrocatechol as well as coffee decoction would be useful for the suppression of neurodegeneration and the prevention of the onsets of Alzheimer’s (AD) and Perkinson’s diseases (PD).

  • GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4<sup>+</sup> T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A Expression

    Mashimo M., Fujii T., Ono S., Moriwaki Y., Misawa H., Azami T., Kasahara T., Kawashima K.

    International Journal of Molecular Sciences (International Journal of Molecular Sciences)  24 ( 15 )  2023.07

    Research paper (scientific journal), Accepted,  ISSN  16616596

    　View Summary

    Immune cells such as T cells and macrophages express α7 nicotinic acetylcholine receptors (α7 nAChRs), which contribute to the regulation of immune and inflammatory responses. Earlier findings suggest α7 nAChR activation promotes the development of regulatory T cells (Tregs) in mice. Using human CD4+ T cells, we investigated the mRNA expression of the α7 subunit and the human-specific dupα7 nAChR subunit, which functions as a dominant-negative regulator of ion channel function, under resting conditions and T cell receptor (TCR)-activation. We then explored the effects of the selective α7 nAChR agonist GTS-21 on proliferation of TCR-activated T cells and Treg development. Varied levels of mRNA for both the α7 and dupα7 nAChR subunits were detected in resting human CD4+ T cells. mRNA expression of the α7 nAChR subunit was profoundly suppressed on days 4 and 7 of TCR-activation as compared to day 1, whereas mRNA expression of the dupα7 nAChR subunit remained nearly constant. GTS-21 did not alter CD4+ T cell proliferation but significantly promoted Treg development. These results suggest the potential ex vivo utility of GTS-21 for preparing Tregs for adoptive immunotherapy, even with high expression of the dupα7 subunit.

  • Regulation of immune functions by non-neuronal acetylcholine (ACh) via muscarinic and nicotinic ACh receptors.

    Mashimo M, Moriwaki Y, Misawa H, Kawashima K, Fujii T.

    International Journal of Molecular Sciences (MDPI)  22 ( 13 ) 6818 2021.06

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  16616596

    　View Summary

    Acetylcholine (ACh) is the classical neurotransmitter in the cholinergic nervous system. However, ACh is now known to regulate various immune cell functions. In fact, T cells, B cells, and macrophages all express components of the cholinergic system, including ACh, muscarinic, and nicotinic ACh receptors (mAChRs and nAChRs), choline acetyltransferase, acetylcholinesterase, and choline transporters. In this review, we will discuss the actions of ACh in the immune system. We will first briefly describe the mechanisms by which ACh is stored in and released from immune cells. We will then address Ca2+ signaling pathways activated via mAChRs and nAChRs on T cells and B cells, highlighting the importance of ACh for the function of T cells, B cells, and macrophages, as well as its impact on innate and acquired (cellular and humoral) immunity. Lastly, we will discuss the effects of two peptide ligands, secreted lymphocyte antigen-6/urokinase-type plasminogen activator receptor-related peptide-1 (SLURP-1) and hippocampal cholinergic neurostimulating peptide (HCNP), on cholinergic activity in T cells. Overall, we stress the fact that ACh does not function only as a neurotransmitter; it impacts immunity by exerting diverse effects on immune cells via mAChRs and nAChRs.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • ニコチン受容体修飾因子SLURP-1による乾癬の新規治療戦略

    森脇康博

    臨床免疫・アレルギー科 66 ( 1 ) 45 - 49 2016.07

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

  • パーキンソン病と小胞体ストレス

    森脇康博, 高橋良輔

    現代医療 36 ( 4 ) 129 - 134 2004.04

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

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Presentations 【 Display / hide 】

  • ニコチン性アセチルコリン受容体調節因子 Ly6H の脳内発現領域の同定

    岩瀬陸人, 森﨑祐太, 揣雅涵, 三澤日出巳, 辻祥太郎, 井上直和, 田中謙二, 森脇康博

    [Domestic presentation]  日本薬学会第146年会 (大阪) , 

    2026.03
    , 

    Poster presentation

  • Effects of genetic manipulation of Ly6H, a novel inhibitory regulator of nicotinic acetylcholine receptors, on behavior

    森脇康博, 田邊夏乃, 外塚貴子, 塚原明洋, 森﨑祐太, 三澤日出巳, 井上直和, 辻祥太郎, 兎田幸司

    [Domestic presentation]  動物心理学会第85会大会 (京都) , 

    2025.10
    , 

    Poster presentation

  • Genetic manipulation of Ly6H, a novel inhibitory regulator of nicotinic acetylcholine receptors, affects learning and memory in young and aged mice

    外塚貴子, 井原宥, 佐藤桃, 小林徹郎, 井上直和, 兎田幸司, 森脇康博

    [Domestic presentation]  動物心理学会第85会大会 (京都) , 

    2025.10
    , 

    Poster presentation

  • Can vision-based approach assess mice tremors? Mice tremor level estimation via image-based animal pose tracking

    越元陽太，塚原明洋，森脇康博，五十川麻里子

    [Domestic presentation]  28th Meeting on Image Recognition and Understanding (京都) , 

    2025.07
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    2025.08
    , 

    Poster presentation

  • 動物姿勢推定モデルを用いたマウスの振戦度評価手法の提案

    越元陽太，塚原明洋，森脇康博，五十川麻里子

    [Domestic presentation]  第48会日本神経科学大会 (新潟) , 

    2025.07
    , 

    Poster presentation

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 革新的バイオセパレーションを実現する機能性高分子修飾ナノ界面の創製

    2024.04
    -
    2028.03

    文部科学省・日本学術振興会, 基盤研究(B), Coinvestigator(s)

  • ニコチン受容体機能抑制タンパク質を標的とした認知症治療戦略の構築

    2024.04
    -
    2027.03

    喫煙科学研究財団, 一般研究, No Setting, Principal investigator

  • 尿路上皮癌を標的としたバイパラトピック抗体医薬品の開発

    2024.04
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    2025.03

    慶應義塾学事振興資金, 個人研究 特B, No Setting, Principal investigator

  • 次世代抗体医薬品の開発を加速するRI標識に関する基盤技術開発とRI標識抗体医薬の実用化研究

    2023.04
    -
    Present

    国立研究開発法人日本医療研究開発機構(AMED), 次世代治療・診断実現のための創薬基盤技術開発事業, Coinvestigator(s)

  • Physiological and pathological roles of functional inhibitors of memory-related nicotinic receptors on memory.

    2023.04
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    2026.03

    文部科学省・日本学術振興会, 基盤研究(C), Principal investigator

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Awards 【 Display / hide 】

  • HPLC2024, Best Poster Award

    Deura K, Moriwaki Y, Citterio Y, Hiruta Y., 2024.07, HPLC2024, Antibody purification using synthetic polymer column packing materials with peptidomimetic molecular design

    Type of Award： Award from international society, conference, symposium, etc.,  Country： United States

  • 第137回 日本薬理学会関東部会　若手優秀発表賞

    渡邉みずほ, 森脇康博, 久保那月, 加藤総夫, 三澤日出巳., 2017.10, 内在性神経毒素類似タンパク質Ly6Hによるニコチン受容体調整.

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • 第131回 日本薬理学会関東部会　若手優秀発表賞

    森﨑祐太, 坪田充司, 森脇康博, 山中宏二, 三澤日出巳., 2014.10, 筋萎縮性側索硬化症における運動ニューロン変性とオステオポンチン及びマトリックスメタロプロテアーゼ-9の関連の検討.

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • RESEARCH FRONTIERS IN BIOMEDICAL SCIENCE

    2025

  • RESEARCH FOR BACHELOR'S THESIS 1

    2025

  • RESEARCH APPARATUS LABORATORY COURSE

    2025

  • PRIOR LEARNING FOR CLINICAL PRACTICE 3

    2025

  • PHYSICAL CHEMISTRY 3

    2025

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Academic Activities 【 Display / hide 】

  • Frontiers in Immunology Review Editor

    2022.06
    -
    Present

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Memberships in Academic Societies 【 Display / hide 】

  • 日本分子生物学会, 

    2019.04
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    Present

  • 日本癌学会, 

    2019.04
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    Present

  • 北米神経科学学会, 

    2015.10
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    Present

  • 日本薬理学会　学術評議員, 

    2013.04
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    Present

  • 日本薬学会, 

    2011.04
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    Present

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Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    2019.03

    ファルマシアトピックス小委員, 日本薬学会

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