中澤　洋介 (ナカザワ　ヨウスケ)

Nakazawa, Yosuke

写真a

所属（所属キャンパス）

薬学部 薬学科 衛生化学講座 （芝共立）

職名

専任講師

メールアドレス

メールアドレス

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経歴 【 表示 ／ 非表示 】

  • 2005年04月
    -
    2009年03月

    摂南大学薬学部, 助手

  • 2009年04月
    -
    2012年03月

    慶應義塾大学薬学部, 助手

  • 2012年04月
    -
    2019年03月

    慶應義塾大学薬学部, 助教

  • 2016年09月
    -
    2017年08月

    The University of Auckland, Faculty of Medical and Health Sciences, Visiting Researcher

  • 2019年04月
    -
    継続中

    慶應義塾大学薬学部, 専任講師

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免許・資格 【 表示 ／ 非表示 】

  • 薬剤師, 2003年04月

  • 健康食品管理士, 2019年01月

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論文 【 表示 ／ 非表示 】

  • Oral Intake of Alpha-Glucosyl-Hesperidin Ameliorates Selenite-Induced Cataract Formation

    Yosuke Nakazawa, Miki Aoki, Sho Ishiwa, Naoki Morishita, Shin Endo, Noriaki Nagai, Naoki Yamspamoto, Megumi Funakoshi-Tago, Hiroomi Tamura

    Molecular Medicine Reports In press 2019年11月

    研究論文（学術雑誌）, 共著, 査読有り

  • Combination with l-Menthol Enhances Transdermal Penetration of Indomethacin Solid Nanoparticles

    Nagai N., Ogata F., Yamaguchi M., Fukuoka Y., Otake H., Nakazawa Y., Kawasaki N.

    International journal of molecular sciences （International journal of molecular sciences)  20   3644 - 3644 2019年07月

    研究論文（学術雑誌）, 査読有り,  ISSN  16616596

    　概要を見る

    This study designed the transdermal formulations containing indomethacin (IMC)-1% IMC was crushed with 0.5% methylcellulose and 5% 2-hydroxypropyl-β-cyclodextrin by the bead mill method, and the milled IMC was gelled with or without 2% l-menthol (a permeation enhancer) by Carbopol® 934 (without menthol, N-IMC gel; with menthol, N-IMC/MT gel). In addition, the drug release, skin penetration and percutaneous absorption of the N-IMC/MT gel were investigated. The particle sizes of N-IMC gel were approximately 50-200 nm, and the combination with l-menthol did not affect the particle characterization of the transdermal formulations. In an in vitro experiment using a Franz diffusion cell, the skin penetration in N-IMC/MT gel was enhanced than the N-IMC gel, and the percutaneous absorption (AUC) from the N-IMC/MT gel was 2-fold higher than the N-IMC gel. On the other hand, the skin penetration from the N-IMC/MT gel was remarkably attenuated at a 4 °C condition, a temperature that inhibits all energy-dependent endocytosis. In conclusion, this study designed transdermal formulations containing IMC solid nanoparticles and l-menthol, and found that the combination with l-menthol enhanced the skin penetration of the IMC solid nanoparticles. In addition, the energy-dependency of the skin penetration of IMC solid nanoparticles was demonstrated. These findings suggest the utility of a transdermal drug delivery system to provide the easy application of solid nanoparticles (SNPs).

  • Verification and spatial mapping of TRPV1 and TRPV4 expression in the embryonic and adult mouse lens

    Nakazawa Y., Donaldson P., Petrova R.

    Experimental Eye Research （Experimental Eye Research)  186   107707 - 107707 2019年06月

    研究論文（学術雑誌）, 査読有り,  ISSN  00144835

    　概要を見る

    © 2019 The transient receptor protein vanilloid channels, TRPV1 and TRPV4, have recently been shown to be mechanosensors in the ocular lens that act to transduce physical changes in lens volume and internal hydrostatic pressure into the activation of signalling pathways in lens epithelial cells. These pathways in turn regulate ion and water transport to ensure that the optical properties of the lens remain constant. Despite the functional evidence that implicate the roles of TRPV1 and TRPV4 in the lens, their respective cellular expression patterns in the different regions of the lens has to date not been fully characterised. Using Western blotting we have confirmed that TRPV1 and TRPV4 are expressed throughout all regions (epithelium, outer cortex, inner cortex/core) of the adult mouse lens. Subsequent immunolabeling of lens cryosections confirmed that TRPV1 and TRPV4 are expressed throughout all regions of the lens, but revealed differentiation-dependent differences in the subcellular expression of the two channels in the different regions. In the epithelium and outer cortex, intense TRPV1 and TRPV4 labeling was predominately associated with the cytoplasm. In a discrete zone in the inner cortex, labeling for both proteins was greatly diminished, but could be enhanced by incubating sections with the detergent Triton X-100 to reveal TRPV1 and TRPV4 labelling that was associated with the membrane. This suggests that in this region of the lens there is a potential interacting protein that masks the binding of the TRPV1 and TRPV4 antibodies to their respective epitopes in the lens inner cortex. In the core of the lens, which contains the embryonic nucleus, TRPV1 and TRPV4 labelling was associated exclusively with fibre cell membranes. This labelling in the lens core of the adult mouse lens appeared to originate in early development as a similar membrane labelling was observed at embryonic day 10 (E10) of the cells in the lens vesicle that subsequently forms the embryonic nucleus in the adult lens. During subsequent stages of embryonic development TRPV1 and TRPV4 remained membranous in the inner cortex and core, while showing labelling that was associated with the cytoplasm in the superficial outer cortical region. The extent of cytoplasmic labelling for TRPV4, but not TRPV1, in this cortical region could however be dynamically regulated by cutting the zonules that normally attach the lens to the ciliary body. We have shown an early onset and continuous expression of TRPV1 and TRPV4 across all lens regions, and that TRPV4 can be dynamically trafficked into the membranes of differentiating fibre cells, results that suggests that these mechanosensitive channels may also be functionally active in lens fibre cells.

  • Stability of 2-Amino-3-hydroxyacetophenone-O-beta-D-glucoside Against Near-UV Irradiation

    Mikako Oka, Makoto Takehana, Yosuke Nakazawa, Hiroko Ushikubo, Kenshi Satoh

    Journal of the Japanese Society for Cataract Research 31 （ 1 ） 58 - 62 2019年06月

    研究論文（学術雑誌）, 共著, 査読有り

  • Energy-dependent endocytosis is responsible for drug transcorneal penetration following the instillation of ophthalmic formulations containing indomethacin nanoparticles

    Nagai N., Ogata F., Otake H., Nakazawa Y., Kawasaki N.

    International Journal of Nanomedicine （International Journal of Nanomedicine)  14   1213 - 1227 2019年02月

    研究論文（学術雑誌）,  ISSN  11769114

    　概要を見る

    © 2019 Nagai et al. Purpose: We previously found that ophthalmic formulations containing nanoparticles prepared by a bead mill method lead to an increase in bioavailability in comparison with traditional formulations (solution type). However, the transcorneal penetration pathway for ophthalmic formulations has not been explained yet. In this study, we investigated the mechanism of transcorneal penetration in the application of ophthalmic formulations containing indomethacin nanoparticles (IMC-NPs). Materials and methods: IMC-NPs was prepared by the bead mill method. For the analysis of energy-dependent endocytosis, corneal epithelial (HCE-T) cell monolayers and removed rabbit cornea were thermoregulated at 4°C, where energy-dependent endocytosis is inhibited. In addition, for the analysis of different endocytosis pathways using pharmacological inhibitors, inhibitors of caveolae-mediated endocytosis (54 µM nystatin), clathrin-mediated endocytosis (40 µM dynasore), macropinocytosis (2 µM rottlerin) or phagocytosis (10 µM cytochalasin D) were used. Results: The ophthalmic formulations containing 35–200 nm sized indomethacin nanoparticles were prepared by treatment with a bead mill, and no aggregation or degradation of indomethacin was observed in IMC-NPs. The transcorneal penetration of indomethacin was significantly decreased by the combination of nystatin, dynasore and rottlerin, and the decreased penetration levels were similar to those at 4°C in HCE-T cell monolayers and rabbit cornea. In the in vivo experiments using rabbits, dynasore and rottlerin tended to decrease the transcorneal penetration of indomethacin (area under the drug concentration – time curve in the aqueous humor [AUC AH ]), and the AUC AH in the nystatin-treated rabbit was significantly lower than that in non-treatment group. In addition, the AUC AH in rabbit corneas undergoing multi-treatment was obviously lower than that in rabbit corneas treated with each individual endocytosis inhibitor. Conclusion: We found that three energy-dependent endocytosis pathways (clathrin-dependent endocytosis, caveolae-dependent endocytosis and macropinocytosis) are related to the transcorneal penetration of indomethacin nanoparticles. In particular, the caveolae-dependent endocytosis is strongly involved.

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KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

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総説・解説等 【 表示 ／ 非表示 】

  • フィレンシンによるアクアポリン0(AQP0)の機能解析.

    中澤洋介.

    日本白内障学会誌 (日本白内障学会)  26   28-29 2014年06月

    研究論文, 単著

  • 水晶体中のRNAの解析.

    岡 美佳子, 梅澤 和寛, 中澤 洋介, 竹鼻 眞.

    日本眼科学会雑誌 (日本眼科学会)  117(10)   826-826 2013年10月

    総説・解説（学術雑誌）, 共著

  • 水晶体線維細胞分化に伴う遺伝子発現の変化.

    梅澤 和寛, 岡 美佳子, 中澤 洋介, 竹鼻 眞.

    日本眼科学会雑誌 (日本眼科学会)  115(9)   848-848 2011年09月

    総説・解説（学術雑誌）, 共著

  • 糖尿病性白内障発症前後でのアスコルビン酸トランスポーターの発現解析.

    中澤洋介, 岡美佳子, 竹鼻眞.

    日本眼科学会雑誌 (日本眼科学会)  114   809-809 2010年09月

    総説・解説（学術雑誌）, 共著

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研究発表 【 表示 ／ 非表示 】

  • The subcellular localisation of AQP5: is it regulated by zonular tension and/or the activation of TRPV1 and 4 ion channels?

    Rosica S. Petrova, Nandini Bavana, Rusin Zhao, Yosuke Nakazawa, Kevin L. Schey, Paul J. Donaldson

    International Conference on the Lens 2019, 2019年12月, 口頭（一般）

  • Aquaporin 0a is required for water homeostasis in the zebrafish lens in vivo

    Irene Vorontsova, Alexander Vallmitjana, Yosuke Nakazawa, Belén Torrado, Thomas F. Schilling, James E. Hall, Enrico Gratton, Leonel Malacrida,

    International Conference on the Lens 2019, 2019年12月, 口頭（一般）

  • Pharmacological modulation and changes in osmolarity alter the subcellular distribution of TRPV1 and TRPV4 channels in the mouse lens

    Yosuke Nakazawa, Yuki Sughiyama, Rosica S. Petrova, Noriaki Nagai, Paul J. Donaldson, Hiroomi Tamura

    International Conference on the Lens 2019, 2019年12月, 口頭（一般）

  • 水晶体に発現しているTRPV1およびTRPV4の局在性を決める因子の同定

    中澤洋介、杉山裕紀、Rosica Petrova, Paul Donaldson, 多胡めぐみ、田村悦臣

    第92回　日本生化学会大会, 2019年09月, 口頭（一般）

  • 抗白内障薬／抗老眼薬の創製を見据えて： 水晶体透明性維持機構の解明と疾患予防の基盤研究

    中澤　洋介

    第63回　日本薬学会関東支部大会, 2019年09月, 口頭（招待・特別）

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競争的資金等の研究課題 【 表示 ／ 非表示 】

  • 老眼発症遅延薬の開発を見据えて：老眼発症メカニズムの解明と老眼モデル動物の開発

    2018年09月
    -
    2019年08月

    ひと・健康・未来財団, 中澤洋介, 補助金,  代表

  • アクアポリン0およびアクアポリン5の機能解明とこれらを標的とした新規抗白内 障薬の創生

    2016年09月
    -
    2017年08月

    公益財団法人 持田記念医学薬学振興財団, 補助金, 

  • 新規白内障治療薬の開発を目指したアクアポリン0の機能解明

    2016年04月
    -
    2018年03月

    文部科学省, 科学研究費補助金(文部科学省・日本学術振興会）, 中澤洋介, 補助金,  代表

  • 水晶体細胞膜のチャネル及び細胞接着の役割を担うアクアポリン0の機能制御機構の解明

    2014年04月
    -
    2016年03月

    文部科学省, 科学研究費補助金(文部科学省・日本学術振興会）, 中澤洋介, 補助金,  代表

  • 水晶体透明性維持機構の解明と白内障予防

    2012年04月
    -
    2013年03月

    慶應義塾大学　学事振興資金, 慶應義塾学事振興資金, 補助金,  代表

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受賞 【 表示 ／ 非表示 】

  • 日本薬学会2019年度関東支部 奨励賞

    中澤洋介, 2019年09月, 公益社団法人日本薬学会関東支部, 抗白内障薬／抗老眼薬の創製を見据えて：水晶体透明性維持機構の解明と疾患予防の基盤研究

    受賞区分： 出版社・新聞社・財団等の賞,  受賞国： 日本

  • 日本白内障学会 学術賞

    中澤洋介, 2014年09月, 日本白内障学会, 水晶体のアクアポリン0の役割および機能に関する新しい知見

  • 2019年度　助成研究発表会　優秀賞

    2019年11月, 公益財団法人　ひと・健康・未来研究財団

    受賞区分： 国内学会・会議・シンポジウム等の賞

  • Imaging competion Winner 2019

    2019年07月, Vector Laboratories社, lens E10 section

    受賞区分： 出版社・新聞社・財団等の賞,  受賞国： CA, USA

  • Young Investigator Awards

    Yosuke Nakazawa, Rosica S. Petrova, Hiroomi Tamura, Paul J. Donaldson, 2017年12月, National Foundation for Eye Research, The subcellular expression patterns of the mechano-sensitive channels TRPV1/4 in the mouse lens are modulated by changes in zonular tension

    受賞国： Kona, Hawaii

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担当授業科目 【 表示 ／ 非表示 】

  • 課題研究（衛生化学）

    2019年度

  • 細胞培養・遺伝子実験特別演習

    2019年度

  • 演習（衛生化学）

    2019年度

  • 卒業研究Ａ

    2019年度

  • 薬学英語演習Ｇ

    2019年度

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委員歴 【 表示 ／ 非表示 】

  • 2018年07月

    大会長, 第44回　水晶体研究会　

  • 2018年07月
    -
    継続中

    白内障学会誌　副編集委員長, 日本白内障学会

  • 2012年06月
    -
    継続中

    世話人, 水晶体研究会

  • 2012年06月
    -
    2018年07月

    白内障学会誌　編集委員, 日本白内障学会

  • 2019年07月
    -
    継続中

    評議員, 白内障学会

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