中澤　洋介 (ナカザワ　ヨウスケ)

Nakazawa, Yosuke

写真a

所属（所属キャンパス）

薬学部 薬学科 衛生化学講座 （芝共立）

職名

専任講師

メールアドレス

メールアドレス

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経歴 【 表示 ／ 非表示 】

  • 2005年04月
    -
    2009年03月

    摂南大学薬学部, 助手

  • 2009年04月
    -
    2012年03月

    慶應義塾大学薬学部, 助手

  • 2012年04月
    -
    2019年03月

    慶應義塾大学薬学部, 助教

  • 2016年09月
    -
    2017年08月

    The University of Auckland, Faculty of Medical and Health Sciences, Visiting Researcher

  • 2019年04月
    -
    継続中

    慶應義塾大学薬学部, 専任講師

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免許・資格 【 表示 ／ 非表示 】

  • 薬剤師, 2003年04月

  • 健康食品管理士, 2019年01月

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論文 【 表示 ／ 非表示 】

  • Aquaporin 0A is Required for Water Homeostasis in the Zebrafish Lens In Vivo

    Vorontsova Irene, Vallmitjana Alexander, Nakazawa Yosuke, Torrado Belen, Schilling Thomas, Hall James E., Gratton Enrico, Malacrida Leonel S.

    BIOPHYSICAL JOURNAL （Cell Press)  118 （ 3 ） 167a - 167A 2020年02月

    ISSN  0006-3495

  • Novel sustained‐release drug delivery system for dry eye therapy by rebamipide nanoparticles

    Nagai N., Ishii M., Seiriki R., Ogata F., Otake H., Nakazawa Y., Okamoto N., Kanai K., Kawasaki N.

    Pharmaceutics （Pharmaceutics)  12 （ 2 ）  2020年02月

    　概要を見る

    © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The commercially available rebamipide ophthalmic suspension (CA‐REB) was approved for clinical use in patients with dry eye; however, the residence time on the ocular surface for the traditional formulations is short, since the drug is removed from the ocular surface through the nasolacrimal duct. In this study, we designed a novel sustained‐release drug delivery system (DDS) for dry eye therapy by rebamipide nanoparticles. The rebamipide solid nanoparticle‐based ophthalmic formulation (REB‐NPs) was prepared by a bead mill using additives (2‐hydroxypropyl-β‐cyclodextrin and methylcellulose) and a gel base (carbopol). The rebamipide particles formed are ellipsoid, with a particle size in the range of 40–200 nm. The rebamipide in the REB‐NPs applied to eyelids was delivered into the lacrimal fluid through the meibomian glands, and sustained drug release was observed in comparison with CA‐REB. Moreover, the REB‐NPs increased the mucin levels in the lacrimal fluid and healed tear film breakup levels in an N‐acetylcysteine‐treated rabbit model. The information about this novel DDS route and creation of a nano‐formulation can be used to design further studies aimed at therapy for dry eye.

  • Oral intake of α-glucosyl-hesperidin ameliorates selenite-induced cataract formation

    Nakazawa Y., Aoki M., Ishiwa S., Morishita N., Endo S., Nagai N., Yamamoto N., Funakoshi-Tago M., Tamura H.

    Molecular Medicine Reports （Molecular Medicine Reports)  21 （ 3 ） 1258 - 1266 2020年

    ISSN  17912997

    　概要を見る

    © 2020 Spandidos Publications. All rights reserved. Hesperetin is a natural flavonoid with robust antioxidant properties. Our previous study reported that hesperetin can prevent cataract formation. However, an important consideration regarding hesperetin consumption is the limited bioavailability due to its poor solubility. The present study investigated the anti-cataract effects of α-glucosyl hesperidin in vivo and in vitro using a selenite-induced cataract model. SD rats (age, 13 days) were orally administered PBS (0.2 ml) or α-glucosyl hesperidin (200 mg/kg) on days 0, 1 and 2. Sodium selenite was subcutaneously administered to the rats 4 h after the first oral administration on day 0. Antioxidant levels in the lens and blood were measured on day 6. In vitro, human lens epithelial cells were treated with sodium selenite (10 μM) and/or hesperetin (50 or 100 mM) for 24 h and analyzed for apoptosis markers using sub-G1 population and Annexin V-FITC/propidium iodide staining and DNA ladder formation. α-glucosyl hesperidin treatment significantly reduced the severity of selenite-induced cataract. The level of antioxidants was significantly reduced in the selenite-treated rats compared with in the controls; however, they were normalized with α-glucosyl hesperidin treatment. In vitro, hesperetin could significantly reduce the number of cells undergoing apoptosis induced by sodium selenite in human lens epithelial cell lines. Overall, oral consumption of α-glucosyl hesperidin could delay the onset of selenite-induced cataract, at least in part by modulating the selenite-induced cell death in lens epithelial cells.

  • Combination with l-Menthol Enhances Transdermal Penetration of Indomethacin Solid Nanoparticles

    Nagai N., Ogata F., Yamaguchi M., Fukuoka Y., Otake H., Nakazawa Y., Kawasaki N.

    International journal of molecular sciences （International journal of molecular sciences)  20 （ 15 ） 3644 - 3644 2019年07月

    研究論文（学術雑誌）, 査読有り,  ISSN  16616596

    　概要を見る

    This study designed the transdermal formulations containing indomethacin (IMC)-1% IMC was crushed with 0.5% methylcellulose and 5% 2-hydroxypropyl-β-cyclodextrin by the bead mill method, and the milled IMC was gelled with or without 2% l-menthol (a permeation enhancer) by Carbopol® 934 (without menthol, N-IMC gel; with menthol, N-IMC/MT gel). In addition, the drug release, skin penetration and percutaneous absorption of the N-IMC/MT gel were investigated. The particle sizes of N-IMC gel were approximately 50-200 nm, and the combination with l-menthol did not affect the particle characterization of the transdermal formulations. In an in vitro experiment using a Franz diffusion cell, the skin penetration in N-IMC/MT gel was enhanced than the N-IMC gel, and the percutaneous absorption (AUC) from the N-IMC/MT gel was 2-fold higher than the N-IMC gel. On the other hand, the skin penetration from the N-IMC/MT gel was remarkably attenuated at a 4 °C condition, a temperature that inhibits all energy-dependent endocytosis. In conclusion, this study designed transdermal formulations containing IMC solid nanoparticles and l-menthol, and found that the combination with l-menthol enhanced the skin penetration of the IMC solid nanoparticles. In addition, the energy-dependency of the skin penetration of IMC solid nanoparticles was demonstrated. These findings suggest the utility of a transdermal drug delivery system to provide the easy application of solid nanoparticles (SNPs).

  • Verification and spatial mapping of TRPV1 and TRPV4 expression in the embryonic and adult mouse lens

    Nakazawa Y., Donaldson P., Petrova R.

    Experimental Eye Research （Experimental Eye Research)  186   107707 - 107707 2019年06月

    研究論文（学術雑誌）, 査読有り,  ISSN  00144835

    　概要を見る

    © 2019 The transient receptor protein vanilloid channels, TRPV1 and TRPV4, have recently been shown to be mechanosensors in the ocular lens that act to transduce physical changes in lens volume and internal hydrostatic pressure into the activation of signalling pathways in lens epithelial cells. These pathways in turn regulate ion and water transport to ensure that the optical properties of the lens remain constant. Despite the functional evidence that implicate the roles of TRPV1 and TRPV4 in the lens, their respective cellular expression patterns in the different regions of the lens has to date not been fully characterised. Using Western blotting we have confirmed that TRPV1 and TRPV4 are expressed throughout all regions (epithelium, outer cortex, inner cortex/core) of the adult mouse lens. Subsequent immunolabeling of lens cryosections confirmed that TRPV1 and TRPV4 are expressed throughout all regions of the lens, but revealed differentiation-dependent differences in the subcellular expression of the two channels in the different regions. In the epithelium and outer cortex, intense TRPV1 and TRPV4 labeling was predominately associated with the cytoplasm. In a discrete zone in the inner cortex, labeling for both proteins was greatly diminished, but could be enhanced by incubating sections with the detergent Triton X-100 to reveal TRPV1 and TRPV4 labelling that was associated with the membrane. This suggests that in this region of the lens there is a potential interacting protein that masks the binding of the TRPV1 and TRPV4 antibodies to their respective epitopes in the lens inner cortex. In the core of the lens, which contains the embryonic nucleus, TRPV1 and TRPV4 labelling was associated exclusively with fibre cell membranes. This labelling in the lens core of the adult mouse lens appeared to originate in early development as a similar membrane labelling was observed at embryonic day 10 (E10) of the cells in the lens vesicle that subsequently forms the embryonic nucleus in the adult lens. During subsequent stages of embryonic development TRPV1 and TRPV4 remained membranous in the inner cortex and core, while showing labelling that was associated with the cytoplasm in the superficial outer cortical region. The extent of cytoplasmic labelling for TRPV4, but not TRPV1, in this cortical region could however be dynamically regulated by cutting the zonules that normally attach the lens to the ciliary body. We have shown an early onset and continuous expression of TRPV1 and TRPV4 across all lens regions, and that TRPV4 can be dynamically trafficked into the membranes of differentiating fibre cells, results that suggests that these mechanosensitive channels may also be functionally active in lens fibre cells.

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KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

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総説・解説等 【 表示 ／ 非表示 】

  • フィレンシンによるアクアポリン0(AQP0)の機能解析.

    中澤洋介.

    日本白内障学会誌 (日本白内障学会)  26   28-29 2014年06月

    研究論文, 単著

  • 水晶体中のRNAの解析.

    岡 美佳子, 梅澤 和寛, 中澤 洋介, 竹鼻 眞.

    日本眼科学会雑誌 (日本眼科学会)  117(10)   826-826 2013年10月

    総説・解説（学術雑誌）, 共著

  • 水晶体線維細胞分化に伴う遺伝子発現の変化.

    梅澤 和寛, 岡 美佳子, 中澤 洋介, 竹鼻 眞.

    日本眼科学会雑誌 (日本眼科学会)  115(9)   848-848 2011年09月

    総説・解説（学術雑誌）, 共著

  • 糖尿病性白内障発症前後でのアスコルビン酸トランスポーターの発現解析.

    中澤洋介, 岡美佳子, 竹鼻眞.

    日本眼科学会雑誌 (日本眼科学会)  114   809-809 2010年09月

    総説・解説（学術雑誌）, 共著

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研究発表 【 表示 ／ 非表示 】

  • 水晶体透明性維持機構の解明と関連疾患予防の可能性

    中澤洋介

    日本薬学会　第140年会, 2020年03月, シンポジウム・ワークショップ パネル（指名）

  • Aquaporin 0a is required for water homeostasis in the zebrafish lens in vivo

    Irene Vorontsova, Alexander Vallmitjana, Yosuke Nakazawa, Belén Torrado, Thomas Schilling, James E. Hall, Enrico Gratton, Leonel S. Malacrida.

    Biophysical Society 2020, 64th Annual meeting of the Biophysical Society, 2020年02月, 口頭（一般）

  • The subcellular localisation of AQP5: is it regulated by zonular tension and/or the activation of TRPV1 and 4 ion channels?

    Rosica S. Petrova, Nandini Bavana, Rusin Zhao, Yosuke Nakazawa, Kevin L. Schey, Paul J. Donaldson

    International Conference on the Lens 2019, 2019年12月, 口頭（一般）

  • Aquaporin 0a is required for water homeostasis in the zebrafish lens in vivo

    Irene Vorontsova, Alexander Vallmitjana, Yosuke Nakazawa, Belén Torrado, Thomas F. Schilling, James E. Hall, Enrico Gratton, Leonel Malacrida,

    International Conference on the Lens 2019, 2019年12月, 口頭（一般）

  • Pharmacological modulation and changes in osmolarity alter the subcellular distribution of TRPV1 and TRPV4 channels in the mouse lens

    Yosuke Nakazawa, Yuki Sughiyama, Rosica S. Petrova, Noriaki Nagai, Paul J. Donaldson, Hiroomi Tamura

    International Conference on the Lens 2019, 2019年12月, 口頭（一般）

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競争的資金等の研究課題 【 表示 ／ 非表示 】

  • 老眼発症遅延薬の開発を見据えて：老眼発症メカニズムの解明と老眼モデル動物の開発

    2018年09月
    -
    2019年08月

    ひと・健康・未来財団, 中澤洋介, 補助金,  代表

  • アクアポリン0およびアクアポリン5の機能解明とこれらを標的とした新規抗白内 障薬の創生

    2016年09月
    -
    2017年08月

    公益財団法人 持田記念医学薬学振興財団, 補助金, 

  • 新規白内障治療薬の開発を目指したアクアポリン0の機能解明

    2016年04月
    -
    2018年03月

    文部科学省, 科学研究費補助金(文部科学省・日本学術振興会）, 中澤洋介, 補助金,  代表

  • 水晶体細胞膜のチャネル及び細胞接着の役割を担うアクアポリン0の機能制御機構の解明

    2014年04月
    -
    2016年03月

    文部科学省, 科学研究費補助金(文部科学省・日本学術振興会）, 中澤洋介, 補助金,  代表

  • 水晶体透明性維持機構の解明と白内障予防

    2012年04月
    -
    2013年03月

    慶應義塾大学　学事振興資金, 慶應義塾学事振興資金, 補助金,  代表

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受賞 【 表示 ／ 非表示 】

  • 日本薬学会2019年度関東支部 奨励賞

    中澤洋介, 2019年09月, 公益社団法人日本薬学会関東支部, 抗白内障薬／抗老眼薬の創製を見据えて：水晶体透明性維持機構の解明と疾患予防の基盤研究

    受賞区分： 出版社・新聞社・財団等の賞,  受賞国： 日本

  • 日本白内障学会 学術賞

    中澤洋介, 2014年09月, 日本白内障学会, 水晶体のアクアポリン0の役割および機能に関する新しい知見

  • 2019年度　助成研究発表会　優秀賞

    2019年11月, 公益財団法人　ひと・健康・未来研究財団

    受賞区分： 国内学会・会議・シンポジウム等の賞

  • Imaging competion Winner 2019

    2019年07月, Vector Laboratories社, lens E10 section

    受賞区分： 出版社・新聞社・財団等の賞,  受賞国： CA, USA

  • Young Investigator Awards

    Yosuke Nakazawa, Rosica S. Petrova, Hiroomi Tamura, Paul J. Donaldson, 2017年12月, National Foundation for Eye Research, The subcellular expression patterns of the mechano-sensitive channels TRPV1/4 in the mouse lens are modulated by changes in zonular tension

    受賞国： Kona, Hawaii

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その他 【 表示 ／ 非表示 】

  • 日本薬学会　第140年会 一般シンポジウム　オーガナイザー

    2020年03月

    　内容を見る

    日本薬学会　第140年会
    一般シンポジウム　オーガナイザー

    今いちど，眼科領域の進歩を考える　―基礎，臨床，そしてドラッグリポジショニングについて―

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担当授業科目 【 表示 ／ 非表示 】

  • 課題研究（衛生化学）

    2019年度

  • 細胞培養・遺伝子実験特別演習

    2019年度

  • 演習（衛生化学）

    2019年度

  • 卒業研究Ａ

    2019年度

  • 薬学英語演習Ｇ

    2019年度

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委員歴 【 表示 ／ 非表示 】

  • 2018年07月

    大会長, 第44回　水晶体研究会　

  • 2018年07月
    -
    継続中

    白内障学会誌　副編集委員長, 日本白内障学会

  • 2012年06月
    -
    継続中

    世話人, 水晶体研究会

  • 2012年06月
    -
    2018年07月

    白内障学会誌　編集委員, 日本白内障学会

  • 2019年07月
    -
    継続中

    評議員, 白内障学会

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