Nakazawa, Yosuke



Faculty of Pharmacy, Department of Pharmacy 衛生化学講座 (Shiba-Kyoritsu)


Assistant Professor/Senior Assistant Professor

Career 【 Display / hide

  • 2005.04

    Faculity of pahrmacy, setsunan university, 助手

  • 2009.04

    慶應義塾大学薬学部, 助手

  • 2012.04

    慶應義塾大学薬学部, 助教

  • 2016.09

    The University of Auckland, Faculty of Medical and Health Sciences, Visiting Researcher

  • 2019.04

    Fuculty of Pharmacy, Keio University, Senior Lecturer

Licenses and Qualifications 【 Display / hide

  • 薬剤師, 2003.04

  • 健康食品管理士, 2019.01


Papers 【 Display / hide

  • Identification of Ascorbate Free Radical Reductase in the Lens

    Mikako Oka, Masayasu Bando, Yosuke Nakazawa, Hiroko Ushikubo, Takashi Yoshikado, Kenji Okada, Koji Chiba, Makoto Takehana

    The Journal of the Japanese Society for Cataract Research (Japanese Society for Cataract Research)  36   113 - 122 2024.06

    Research paper (scientific journal), Joint Work, Accepted

  • ニルバジピンナノサスペンション点眼による眼組織への 薬物送達:経口投与時における薬物挙動との比較

    門脇玲太, 明和亮伍, 岩井誓治, 永田万由美, 松島博之, 中澤洋介, 山本直樹, 佐々木 洋, 長井紀章

    日本白内障学会誌 (日本白内障学会)  36   95 - 99 2024.06

    Research paper (scientific journal), Joint Work

  • 亜セレン酸誘発白内障モデルの混濁過程における 水晶体弾性力の変化

    門脇玲太, 浅井拓己, 出口粧央里, 大竹裕子, 岡本紀夫, 中澤洋介, 佐々木 洋, 長井紀章

    日本白内障学会誌 (日本白内障学会)  36   104 - 109 2024.06

    Research paper (scientific journal), Joint Work, Accepted

  • Design of an Oral Tablet Containing Furosemide Nanoparticles with Elevated Bioavailability.

    Nagai N, Ogata F, Kadowaki R, Deguchi S, Otake H, Nakazawa Y, Misra M, Kawasaki N

    Journal of oleo science (Journal of oleo science)  73 ( 4 ) 563 - 571 2024

    ISSN  1345-8957

     View Summary

    The solubility and permeability of the Biopharmaceutics Classification System (BCS) class IV drugs, such as furosemide (FUR), are low. Thus, the oral bioavailability of these drugs needs to be augmented. Here, we aimed to design orally disintegrating tablets containing FUR nanoparticles to improve bioavailability after oral administration. The FUR nanoparticles were generated by bead-milling in water containing 0.5% methylcellulose and 0.5% 2-hydroxypropyl-β-cyclodextrin (w/w%). Particle size was approximately 47-350 nm (mean particle size, 188 nm). An orally disintegrating tablet (FUR-NP tablet) comprising FUR nanoparticles (1%) was successfully produced by employing suspensions outlined above that incorporated additives (4% D-mannitol, 0.4% polyvinylpyrrolidone, and 16% gum Arabic, w/w%), followed by freeze-drying. The FUR-NP tablet disaggregated after only 5 s in water, liberating nano-sized FUR particles (172 nm). Experiments using rats showed the absorption of the FUR-NP tablet was significantly improved by comparison with a FUR tablet containing microparticles. In summary, the orally disintegrating tablet containing FUR nanoparticles markedly enhanced the bioavailability of FUR. We anticipate this formulation will also improve the bioavailability of other BCS class IV drugs.

  • Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia.

    Murata T, Tago K, Miyata K, Moriwaki Y, Misawa H, Kobata K, Nakazawa Y, Tamura H, Funakoshi-Tago M

    International journal of molecular sciences (International Journal of Molecular Sciences)  25 ( 1 )  2023.12

    ISSN  16616596

     View Summary

    According to numerous studies, it has been epidemiologically suggested that habitual coffee intake seems to prevent the onset of neurodegenerative diseases. In this study, we hypothesized that coffee consumption suppresses neuroinflammation, which is closely related to the development of neurodegenerative diseases. Using microglial BV-2 cells, we first found that the inflammatory responses induced by lipopolysaccharide (LPS) stimulation was diminished by both coffee and decaffeinated coffee through the inhibition of an inflammation-related transcription factor, nuclear factor-κB (NF-κB). Pyrocatechol, a component of roasted coffee produced by the thermal decomposition of chlorogenic acid, also exhibited anti-inflammatory activity by inhibiting the LPS-induced activation of NF-κB. Finally, in an inflammation model using mice injected with LPS into the cerebrum, we observed that intake of pyrocatechol as well as coffee decoctions drastically suppressed the accumulation of microglia and the expression of interleukin-6 (IL-6), tumor necrosis factor α (TNFα), CCL2, and CXCL1 in the inflammatory brain. These observations strongly encourage us to hypothesize that the anti-inflammatory activity of pyrocatechol as well as coffee decoction would be useful for the suppression of neurodegeneration and the prevention of the onsets of Alzheimer’s (AD) and Perkinson’s diseases (PD).

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • Role of Aquaporins and Connexins in the Lens and Contribution to Cataract Development

    Kana Aihara, Yosuke Nakazawa

    The Journal of the Japanese Society for Cataract Research (Japanese Society for Cataract Research)  36   82 - 84 2024.06

    Article, review, commentary, editorial, etc. (scientific journal), Last author, Corresponding author

  • Ambient Temperature affects TRPV1 activity in lens Epithelial Cells

    Yumika Kuno, Yosuke Nakazawa

    The Journal of the Japanese Society for Cataract Research (Japanese Society for Cataract Research)  36   69 - 71 2024.06

    Article, review, commentary, editorial, etc. (scientific journal), Last author, Corresponding author

  • Piezo1 チャネルの活性化はTGM2 の活性化を介して 水晶体硬化を発症する

    土岐友理, 中澤洋介, 助川美優

    日本白内障学会誌 (日本白内障学会)  36   124 - 125 2024.06

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work, Corresponding author

  • コーヒー摂取と白内障予防


    食と医療 (講談社)  27   40 - 46 2023.10

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Lead author, Corresponding author

  • alpha-グルコシルヘスペリジンの水晶体に対する機能と老眼サプリメントの可能性


    食と医療 (講談社)  23   59 - 65 2022.11

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work, Lead author, Last author

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Presentations 【 Display / hide

  • The physical changes by aging in the lens and treatment its related diseases.

    Yosuke Nakazawa



    Symposium, workshop panel (public)

  • 基礎研究で老視に挑む!


    第17回 眼抗加齢医学研究会 アップデートセミナー, 


    Symposium, workshop panel (nominated)

  • 動物モデルによる水晶体硬化機序と治療の可能性




    Symposium, workshop panel (nominated)

  • 高温環境ではTRPV1チャネルの活性化を介して老視発症を早める




    Oral presentation (general)

  • Physiological modulation of the crystalline lens water transport changes the stiffness of the ex vivo non-decapsulated bovine lenses measured using a lens spin test system.

    Paul J. Donaldson, Chen Qiu, Dingchang Shi, Yadi Chen, Xingzheng Pan, Matthew Reilly, Yosuke Nakazawa

    International conference on the Lens 2023 (Hawaii, USA) , 


    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Analysis of the mechanism of presbyopia and development of animal models


    Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

     View Summary

    本申請の目的は”①老眼発症メカニズムの解明”および“② 老視モデル動物の作成”であり、本申請研究を老眼の基礎研究の先駆け研究にする。
    本申請研究では、in vitro, ex vivoの実験系を組み合わせて遂行する。in vitro実験系はヒト水晶体上皮細胞株を用いてTRPVチャネル刺激剤で処理し、TRPVチャネルの活性・局在変化と下流シグナルを検討する。ex vivo 実験系は申請者が確立したex vivo擬老眼モデルを用いて人為的に近方視および遠方視の水晶体を再現し、TRPVチャネルの局在や活性を観察する。本申請研究遂行により、老眼発症メカニズムと老眼モデル動物創製を試みる。

  • Mechanobiological analysis of cell-basement membrane interaction during eye lens morphogenesis


    Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), No Setting

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  • Roles for the TRPVs channels in the lens in the initiation of presbyopia


    文部科学省, Grants-in-Aid for Scientific Research, Yosuke Nakazawa, Grant-in-Aid for Scientific Research (C), Research grant, Principal investigator

     View Summary

    In this study, we examined the function of TRPV channels in the lens and contribution for presbyopia onset. We have previously reported the localization of TRPV channels in the lens, and examined the factors that influence their localization in this study. As a result, we found that the localization was changed by changes in external temperature and stimulation pressure from the ciliary muscle. We also found that administration of the anti-cataract candidate compounds hesperetin and water-soluble hesperidin suppressed the decrease in elasticity of the mouse lens due to altering the localization of TRPV channels. Furthermore, we found that Capsaicin, which is TRPV1 agonist, eye drops suppressed the development of secondary cataracts. In the future, we will examine TRPV channels in human lens samples and create the basis for the development of anti-presbyopia drugs.

  • Roles for the mechanosensitive channels (TRPV1 and TRPV4) in the initiation of presb yopia and development of cataract


    JSPS-NZRS, Bilateral joint research project between Japan and New Zealand, Yosuke Nakazawa, Research grant, Principal investigator

  • 老眼発症遅延薬の開発を見据えて:老眼発症メカニズムの解明と老眼モデル動物の開発


    Japan Health Foundation, Yosuke Nakazawa, Research grant, Principal investigator

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Awards 【 Display / hide

  • 日本薬学会2019年度関東支部 奨励賞

    中澤洋介, 2019.09, 公益社団法人日本薬学会関東支部, 抗白内障薬/抗老眼薬の創製を見据えて:水晶体透明性維持機構の解明と疾患予防の基盤研究

    Type of Award: Award from publisher, newspaper, foundation, etc.

  • 日本白内障学会 学術賞

    中澤洋介, 2014.09, Japanese Society for Cataract Research, 水晶体のアクアポリン0の役割および機能に関する新しい知見

  • Imaging competion Winner 2019

    2019.07, Vector Laboratories社, lens E10 section

    Type of Award: Award from publisher, newspaper, foundation, etc.

  • Highly Commended Award- Confocal microscopy

    2016.12, The University of Auckland, Mouse Lens axial sectioning image

    Country: New Zealand

  • Best Author Award - Molecular Medicine Morphology

    Nakazawa Y, Aihara K, Takeda S, Hatsusaka N, Onouchi T, Hiramatsu N, Nagata M, Nagai N, Tago-F M, Yamamoto N, Sasaki H., 2024.07, The japanese Society for clinical Molecular Morphology, Aquaporins contribute to vacuoles formation in Nile grass type II diabetic rats

    Type of Award: Honored in official journal of a scientific society, scientific journal

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Other 【 Display / hide

  • 第一回 Ocular Scientific Meeting 大会長


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    1st Ocular Scientific Meeting, Chief

  • 日本薬学会 第141年会 一般シンポジウム オーガナイザー


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    日本薬学会 第141年会
    一般シンポジウム オーガナイザー

    [S20] 老化と眼疾患ーいつまでも健康な視機能をー

  • 日本薬学会 第140年会 一般シンポジウム オーガナイザー


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    日本薬学会 第140年会
    一般シンポジウム オーガナイザー

    今いちど,眼科領域の進歩を考える ―基礎,臨床,そしてドラッグリポジショニングについて―

  • Conference President, The 44th Annual meeting of the Japanese Society for Crystalline Lens Research.


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    Conference President,
    The 44th Annual meeting of the Japanese Society for Crystalline Lens Research.


Courses Taught 【 Display / hide











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Media Coverage 【 Display / hide

  • 増える老視、将来の薬物治療に期待

    Medical Tribune, 2022.10

Committee Experiences 【 Display / hide

  • 2018.07

    大会長, 第44回 水晶体研究会 

  • 2023.03

    大会長, 第一回 眼科創薬研究会 大会長

  • 2022.04

    理事, 日本老視学会

  • 2022.01

    監事, Ocular Scientific Meeting

  • 2021.11

    The Journal of The Japanese Society for Cataract Research, Editor-in-Chief, 日本白内障学会

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