Nakazawa, Yosuke

写真a

Affiliation

Faculty of Pharmacy, Department of Pharmacy 衛生化学講座 （Shiba-Kyoritsu）

Position

Assistant Professor/Senior Assistant Professor
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Career 【 Display / hide 】

  • 2005.04
    -
    2009.03

    Faculity of pahrmacy, setsunan university, 助手

  • 2009.04
    -
    2012.03

    慶應義塾大学薬学部, 助手

  • 2012.04
    -
    2019.03

    慶應義塾大学薬学部, 助教

  • 2016.09
    -
    2017.08

    The University of Auckland, Faculty of Medical and Health Sciences, Visiting Researcher

  • 2019.04
    -
    Present

    Fuculty of Pharmacy, Keio University, Senior Lecturer

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Licenses and Qualifications 【 Display / hide 】

  • 薬剤師, 2003.04

  • 健康食品管理士, 2019.01

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Papers 【 Display / hide 】

  • Design of an Oral Tablet Containing Furosemide Nanoparticles with Elevated Bioavailability.

    Nagai N, Ogata F, Kadowaki R, Deguchi S, Otake H, Nakazawa Y, Misra M, Kawasaki N

    Journal of oleo science 73 （ 4 ） 563 - 571 2024

    ISSN  1345-8957

  • Suppression of Neuroinflammation by Coffee Component Pyrocatechol via Inhibition of NF-κB in Microglia.

    Murata T, Tago K, Miyata K, Moriwaki Y, Misawa H, Kobata K, Nakazawa Y, Tamura H, Funakoshi-Tago M

    International journal of molecular sciences （International Journal of Molecular Sciences)  25 （ 1 ）  2023.12

    ISSN  16616596

    　View Summary

    According to numerous studies, it has been epidemiologically suggested that habitual coffee intake seems to prevent the onset of neurodegenerative diseases. In this study, we hypothesized that coffee consumption suppresses neuroinflammation, which is closely related to the development of neurodegenerative diseases. Using microglial BV-2 cells, we first found that the inflammatory responses induced by lipopolysaccharide (LPS) stimulation was diminished by both coffee and decaffeinated coffee through the inhibition of an inflammation-related transcription factor, nuclear factor-κB (NF-κB). Pyrocatechol, a component of roasted coffee produced by the thermal decomposition of chlorogenic acid, also exhibited anti-inflammatory activity by inhibiting the LPS-induced activation of NF-κB. Finally, in an inflammation model using mice injected with LPS into the cerebrum, we observed that intake of pyrocatechol as well as coffee decoctions drastically suppressed the accumulation of microglia and the expression of interleukin-6 (IL-6), tumor necrosis factor α (TNFα), CCL2, and CXCL1 in the inflammatory brain. These observations strongly encourage us to hypothesize that the anti-inflammatory activity of pyrocatechol as well as coffee decoction would be useful for the suppression of neurodegeneration and the prevention of the onsets of Alzheimer’s (AD) and Perkinson’s diseases (PD).

  • Recovery from indomethacin-induced gastrointestinal bleeding by treatment with teprenone

    Deguchi, S; Iwakami, A; Tujigiwa, M; Otake, H; Mano, Y; Yamamoto, N; Nakazawa, Y; Misra, M; Nagai, N

    JOURNAL OF PHARMACEUTICAL HEALTH CARE AND SCIENCES （Journal of Pharmaceutical Health Care and Sciences)  9 （ 1 ） 44 2023.11

    Accepted,  ISSN  2055-0294

    　View Summary

    Background: Gastrointestinal injuries caused by nonsteroidal anti-inflammatory drugs (NSAIDs) is a serious side effect in patients with rheumatoid arthritis (RA). However, effective therapeutic strategies have yet to be established. In this study, we investigated the therapeutic effects of teprenone (TEP), a gastric mucosal protective drug, on NSAID-induced gastrointestinal injuries in rats with RA (AA rats). Methods: Gastrointestinal injury was induced by oral administration of indomethacin (IMC), a typical NSAID. TEP was orally administered after IMC-induced gastrointestinal bleeding, and the stomach, jejunum, and ileum were excised. Results: On day 14 of IMC administration, lesion areas in the stomach, jejunum, and ileum were significantly larger in AA rats than in normal rats. When TEP was orally administered to AA rats, the lesion areas in the stomach, jejunum, and ileum significantly decreased compared with those in control rats (IMC-induced AA rats). Therefore, we measured NOS2 mRNA and NO levels, which were significantly decreased in rats with IMC-induced AA after treatment with TEP. Conclusions: These results suggest that the oral administration of TEP may be useful for the treatment of NSAID-induced gastrointestinal injuries in patients with RA.

  • Piezo1 channel causes lens sclerosis via transglutaminase 2 activation.

    Doki Y, Nakazawa Y, Sukegawa M, Petrova RS, Ishida Y, Endo S, Nagai N, Yamamoto N, Funakoshi-Tago M, Donaldson PJ

    Experimental eye research （Experimental Eye Research)  237   109719 2023.11

    Lead author, Corresponding author, Accepted,  ISSN  0014-4835

    　View Summary

    Presbyopia is caused by age-related lenticular hardening, resulting in near vision loss, and it occurs in almost every individual aged ≥50 years. The lens experiences mechanical pressure during for focal adjustment to change its thickness. As lenticular stiffening results in incomplete thickness changes, near vision is reduced, which is known as presbyopia. Piezo1 is a mechanosensitive channel that constantly senses pressure changes during the regulation of visual acuity, and changes in Piezo1 channel activity may contribute to presbyopia. However, no studies have reported on Piezo1 activation or the onset of presbyopia. To elucidate the relevance of Piezo1 activation and cross-linking in the development of presbyopia, we analysed the function of Piezo1 in the lens. The addition of Yoda1, a Piezo1 activator, induced an increase in transglutaminase 2 (TGM2) mRNA expression and activity through the extra-cellular signal-regulated kinase (ERK) 1/2 and c-Jun-NH2-terminal kinase1/2 pathways. In ex vivo lenses, Yoda1 treatment induced γ-crystallin cross-linking via TMG2 activation. Furthermore, Yoda1 eye-drops in mice led to lenticular hardening via TGM2 induction and activation in vivo, suggesting that Yoda1-treated animals could serve as a model for presbyopia. Our findings indicate that this presbyopia-animal model could be useful for screening drugs for lens-stiffening inhibition.

  • The citrus flavonoid, nobiletin inhibits neuronal inflammation by preventing the activation of NF-κB.

    Murata T, Ishiwa S, Lin X, Nakazawa Y, Tago K, Funakoshi-Tago M

    Neurochemistry international （Neurochemistry International)  171   105613 2023.09

    Accepted,  ISSN  0197-0186

    　View Summary

    Nobiletin (5,6,7,8,3′,4′-hexamethoxyflavone) is one of the flavonoids found in shikuwasa, a popular citrus fruit in Okinawa, Japan. It exerts various pharmacological effects, such as anti-tumor, antioxidant, and anti-inflammatory activities. We herein investigated whether nobiletin attenuated lipopolysaccharide (LPS)-induced inflammatory responses in the murine microglial cell line BV-2 and neuroinflammation in mice induced by an intracerebral injection of LPS. In BV-2 cells, nobiletin significantly inhibited the LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) by preventing the mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. Nobiletin also inhibited the LPS-induced mRNA expression of CCL2, CXCL1, IL-6, and TNFα. Nobiletin markedly attenuated the transcriptional activity of the NF-κB p65 subunit without affecting the degradation of IκBα or the nuclear localization of the NF-κB p65 subunit. Nobiletin also inhibited the LPS-induced activation of JNK, but not ERK or p38, in BV-2 cells. Furthermore, the administration of nobiletin significantly suppressed the accumulation of microglia and induction of the mRNA expression of CCL2, CXCL1, IL-6, and TNFα in the murine brain induced by injecting LPS into the striatum. Collectively, these results suggest the potential of nobiletin as a candidate anti-inflammatory drug for the prevention of neuroinflammation.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • Role of Aquaporins and Connexins in the Lens and Contribution to Cataract Development

    Kana Aihara, Yosuke Nakazawa

    The Journal of the Japanese Society for Cataract Research (Japanese Society for Cataract Research)  36 2024.06

    Article, review, commentary, editorial, etc. (scientific journal), Last author, Corresponding author

  • Ambient Temperature affects TRPV1 activity in lens Epithelial Cells

    Yumika Kuno, Yosuke Nakazawa

    The Journal of the Japanese Society for Cataract Research (Japanese Society for Cataract Research)  36 2024.06

    Article, review, commentary, editorial, etc. (scientific journal), Last author, Corresponding author

  • コーヒー摂取と白内障予防

    中澤洋介、多胡めぐみ、田村悦臣

    食と医療 (講談社)  27   40 - 46 2023.10

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Lead author, Corresponding author

  • alpha-グルコシルヘスペリジンの水晶体に対する機能と老眼サプリメントの可能性

    中澤洋介、遠藤伸、森下尚紀

    食と医療 (講談社)  23   59 - 65 2022.11

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work, Lead author, Last author

  • 水晶体の構造特性と関連疾患予防薬の可能性

    中澤洋介

    ファルマシア (日本薬学会)  58 ( 11 ) 1051 - 1055 2022.11

    Lead author, Corresponding author

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Presentations 【 Display / hide 】

  • The physical changes by aging in the lens and treatment its related diseases.

    Yosuke Nakazawa

    日本薬学会第144年会, 

    2024.03
    , 

    Symposium, workshop panel (public)

  • 基礎研究で老視に挑む！

    中澤洋介

    第17回 眼抗加齢医学研究会 アップデートセミナー, 

    2024.03
    , 

    Symposium, workshop panel (nominated)

  • 動物モデルによる水晶体硬化機序と治療の可能性

    中澤洋介

    第2回日本老視学会, 

    2024.01
    , 

    Symposium, workshop panel (nominated)

  • 高温環境ではTRPV1チャネルの活性化を介して老視発症を早める

    中澤洋介、工野由美香、嶋田響、長井紀章、平松範子、武田駿、山本直樹、多胡めぐみ、佐々木洋

    第2回日本老視学会, 

    2024.01
    , 

    Oral presentation (general)

  • Physiological modulation of the crystalline lens water transport changes the stiffness of the ex vivo non-decapsulated bovine lenses measured using a lens spin test system.

    Paul J. Donaldson, Chen Qiu, Dingchang Shi, Yadi Chen, Xingzheng Pan, Matthew Reilly, Yosuke Nakazawa

    International conference on the Lens 2023 (Hawaii, USA) , 

    2023.12
    , 

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Analysis of the mechanism of presbyopia and development of animal models

    2023.04
    -
    2026.03

    Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

    　View Summary

    本申請の目的は”①老眼発症メカニズムの解明”および“② 老視モデル動物の作成”であり、本申請研究を老眼の基礎研究の先駆け研究にする。
    本申請研究では、in vitro, ex vivoの実験系を組み合わせて遂行する。in vitro実験系はヒト水晶体上皮細胞株を用いてTRPVチャネル刺激剤で処理し、TRPVチャネルの活性・局在変化と下流シグナルを検討する。ex vivo 実験系は申請者が確立したex vivo擬老眼モデルを用いて人為的に近方視および遠方視の水晶体を再現し、TRPVチャネルの局在や活性を観察する。本申請研究遂行により、老眼発症メカニズムと老眼モデル動物創製を試みる。

  • Mechanobiological analysis of cell-basement membrane interaction during eye lens morphogenesis

    2021.04
    -
    2022.03

    Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), No Setting

    　View Summary

    眼の水晶体の主な部分は水晶体線維細胞でできており、これら細胞は水晶体嚢とよばれる強靱な繊維膜の袋の中に入っている。水晶体線維細胞は水晶体嚢の内部で秩序だった同心円層をなし球形を作っている。水晶体が光を屈折させ網膜上に焦点を結ぶ働きをするために、水晶体は正確な球状に形成される必要があるが、その形成メカニズムはよくわかっていない。本研究では水晶体線維細胞と水晶体嚢の力学的相互作用を解析することにより、水晶体の形態形成メカニズムを明らかにする。正確な球形の形成がどのようなメカニズムで制御されるのか明らかにすることは、水晶体の再生技術の開発に役立つことが期待される。
    水晶体が発生の過程でどのようなメカニズムで球状に形成されていくのか知ることは、再生医学の観点からも重要である。水晶体は主に同心円状に並ぶ水晶体線維細胞の層と、それらを包む水晶体嚢と呼ばれる基底膜から構成されている。水晶体嚢は強靱な膜であり、弾力性のある水晶体線維細胞を加圧して包んでいる可能性がある。本研究では、この水晶体嚢と水晶体線維細胞間での力学的相互作用が、どのように水晶体の球形の形成と維持に関わっているのか、マウス水晶体を用いて解析した。我々は水晶体嚢を除去し加圧を解放すると、線維細胞層は均一に拡張するのではなく、特定方向により大きく膨張することを発見した。このことから水晶体嚢による加圧は一様ではなく、水晶体の特定の領域でより強く圧力がかかっていることが示唆された。実際に原子間力顕微鏡で水晶体表面の弾性力を測定すると、領域間での違いが観察された。これらの結果から、水晶体嚢からの加圧の程度が領域により異なるために、哺乳類の水晶体は真円の球体ではなく、赤道方向の直径が前極-後極軸よりも長い押しつぶされた形（いわゆるレンズの形）になることが示唆された。よって水晶体嚢と線維細胞間の力学的相互作用により、水晶体各領域の曲率が調整されていると考えられる。さらに我々は、水晶体線維細胞の弾性力の生成に細胞膜脂質が関与していることを見いだした。今後は細胞膜脂質の合成を阻害した水晶体を用いて、それがどのように水晶体全体の形や硬さに影響するか解析していく。水晶体の硬さは、加齢による硬化が原因の老視（老眼）と深く関わっている。よって細胞膜脂質と水晶体硬化の関連を明らかにすることは、老視の治療方法の開発に向けても重要である。

  • Roles for the TRPVs channels in the lens in the initiation of presbyopia

    2020.04
    -
    2023.03

    文部科学省, Grants-in-Aid for Scientific Research, Yosuke Nakazawa, Grant-in-Aid for Scientific Research (C), Research grant, Principal investigator

    　View Summary

    In this study, we examined the function of TRPV channels in the lens and contribution for presbyopia onset. We have previously reported the localization of TRPV channels in the lens, and examined the factors that influence their localization in this study. As a result, we found that the localization was changed by changes in external temperature and stimulation pressure from the ciliary muscle. We also found that administration of the anti-cataract candidate compounds hesperetin and water-soluble hesperidin suppressed the decrease in elasticity of the mouse lens due to altering the localization of TRPV channels. Furthermore, we found that Capsaicin, which is TRPV1 agonist, eye drops suppressed the development of secondary cataracts. In the future, we will examine TRPV channels in human lens samples and create the basis for the development of anti-presbyopia drugs.

  • Roles for the mechanosensitive channels (TRPV1 and TRPV4) in the initiation of presb yopia and development of cataract

    2020.04
    -
    2022.03

    JSPS-NZRS, Bilateral joint research project between Japan and New Zealand, Yosuke Nakazawa, Research grant, Principal investigator

  • 老眼発症遅延薬の開発を見据えて：老眼発症メカニズムの解明と老眼モデル動物の開発

    2018.09
    -
    2019.08

    Japan Health Foundation, Yosuke Nakazawa, Research grant, Principal investigator

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Awards 【 Display / hide 】

  • 日本薬学会2019年度関東支部 奨励賞

    中澤洋介, 2019.09, 公益社団法人日本薬学会関東支部, 抗白内障薬／抗老眼薬の創製を見据えて：水晶体透明性維持機構の解明と疾患予防の基盤研究

    Type of Award： Award from publisher, newspaper, foundation, etc.

  • 日本白内障学会 学術賞

    中澤洋介, 2014.09, Japanese Society for Cataract Research, 水晶体のアクアポリン0の役割および機能に関する新しい知見

  • 2019年度　助成研究発表会　優秀賞

    2019.11, Japan Health Fundation

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • Imaging competion Winner 2019

    2019.07, Vector Laboratories社, lens E10 section

    Type of Award： Award from publisher, newspaper, foundation, etc.

  • Young Investigator Awards

    Yosuke Nakazawa, Rosica S. Petrova, Hiroomi Tamura, Paul J. Donaldson, 2017.12, National Foundation for Eye Research, The subcellular expression patterns of the mechano-sensitive channels TRPV1/4 in the mouse lens are modulated by changes in zonular tension

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Other 【 Display / hide 】

  • 第一回　Ocular Scientific Meeting 大会長

    2023年03月

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    1st Ocular Scientific Meeting, Chief

  • 日本薬学会　第141年会 一般シンポジウム　オーガナイザー

    2021年03月

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    日本薬学会　第141年会
    一般シンポジウム　オーガナイザー

    [S20] 老化と眼疾患ーいつまでも健康な視機能をー

  • 日本薬学会　第140年会 一般シンポジウム　オーガナイザー

    2020年03月

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    日本薬学会　第140年会
    一般シンポジウム　オーガナイザー

    今いちど，眼科領域の進歩を考える　―基礎，臨床，そしてドラッグリポジショニングについて―

  • Conference President, The 44th Annual meeting of the Japanese Society for Crystalline Lens Research.

    2018年07月

    　View Details

    Conference President,
    The 44th Annual meeting of the Japanese Society for Crystalline Lens Research.

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Courses Taught 【 Display / hide 】

  • PHARMACEUTICAL SCIENCE OF BIOLOGY

    2024

  • HYGIENIC CHEMISTRY AND PUBLIC HEALTH LABORATORY COURSE

    2024

  • HEALTH FOOD SCIENCE

    2024

  • ENVIRONMENTAL HEALTH SCIENCE

    2024

  • ENGLISH EXERCISES FOR PHARMACEUTICAL SCIENCES

    2024

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Media Coverage 【 Display / hide 】

  • 増える老視、将来の薬物治療に期待

    Medical Tribune, 2022.10

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Committee Experiences 【 Display / hide 】

  • 2018.07

    大会長, 第44回　水晶体研究会　

  • 2023.03

    大会長, 第一回　眼科創薬研究会 大会長

  • 2022.04
    -
    Present

    理事, 日本老視学会

  • 2022.01
    -
    Present

    監事, Ocular Scientific Meeting

  • 2021.11
    -
    Present

    The Journal of The Japanese Society for Cataract Research, Editor-in-Chief, 日本白内障学会

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