Nakazawa, Yosuke



Faculty of Pharmacy, Department of Pharmacy 衛生化学講座 (Shiba-Kyoritsu)


Assistant Professor/Senior Assistant Professor

E-mail Address

E-mail address

Career 【 Display / hide

  • 2005.04

    Faculity of pahrmacy, setsunan university, 助手

  • 2009.04

    慶應義塾大学薬学部, 助手

  • 2012.04

    慶應義塾大学薬学部, 助教

  • 2016.09

    The University of Auckland, Faculty of Medical and Health Sciences, Visiting Researcher

  • 2019.04

    Fuculty of Pharmacy, Keio University, Senior Lecturer

Licenses and Qualifications 【 Display / hide

  • 薬剤師, 2003.04

  • 健康食品管理士, 2019.01


Papers 【 Display / hide

  • Prevention of postprandial hyperglycemia by ophthalmic nanoparticles based on protamine zinc insulin in the rabbit

    Deguchi S., Ogata F., Isaka T., Otake H., Nakazawa Y., Kawasaki N., Nagai N.

    Pharmaceutics (Pharmaceutics)  13 ( 3 )  2021.03

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    Postprandial hyperglycemia, a so-called blood glucose spike, is associated with enhanced risks of diabetes mellitus (DM) and its complications. In this study, we attempted to design nanopar-ticles (NPs) of protamine zinc insulin (PZI) by the bead mill method, and prepare ophthalmic formulations based on the PZI-NPs with (nPZI/P) or without polyacrylic acid (nPZI). In addition, we investigated whether the instillation of the newly developed nPZI and nPZI/P can prevent postprandial hyperglycemia in a rabbit model involving the oral glucose tolerance test (OGTT). The particle size of PZI was decreased by the bead mill to a range for both nPZI and nPZI/P of 80–550 nm with no observable aggregation for 6 d. Neither nPZI nor nPZI/P caused any noticeable corneal toxicity. The plasma INS levels in rabbits instilled with nPZI were significantly higher than in rabbits instilled with INS suspensions (commercially available formulations, CA-INS), and the plasma INS levels were further enhanced with the amount of polyacrylic acid in the nPZI/P. In addition, the rapid rise in plasma glucose levels in OGTT-treated rabbits was prevented by a single instillation of nPZI/P, which was significantly more effective at attenuating postprandial hyperglycemia (blood glucose spike) in comparison with nPZI. In conclusion, we designed nPZI/P, and show that a single instillation before OGTT attenuates the rapid enhancement of plasma glucose levels. These findings suggest a better management strategy for the postprandial blood glucose spike, which is an important target of DM therapy.

  • Effect of Alpha-Glucosyl-Hesperidin Consumption on Lens Sclerosis and Presbyopia

    Yosuke Nakazawa, Yuri Doki, Yuki Sugiyama, Ryota Kobayashi, Noriaki Nagai, Naoki Morishita, Shin Endo, Megumi Funakoshi-Tago, Hiroomi Tamura

    Cells (Cells)  10 ( 2 ) 382 - 382 2021.02

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Presbyopia is characterized by a decline in the ability to accommodate the lens. The most commonly accepted theory for the onset of presbyopia is an age-related increase in the stiffness of the lens. However, the cause of lens sclerosis remains unclear. With age, water microcirculation in the lens could change because of an increase in intracellular pressure. In the lens, the intracellular pressure is controlled by the Transient Receptor Potential Vanilloid (TRPV) 1 and TRPV4 feedback pathways. In this study, we tried to elucidate that administration of α-glucosyl-hesperidin (G-Hsd), previously reported to prevent nuclear cataract formation, affects lens elasticity and the distribution of TRPV channels and Aquaporin (AQP) channels to meet the requirement of intracellular pressure. As a result, the mouse control lens was significantly toughened compared to both the 1% and 2% G-Hsd mouse lens treatments. The anti-oxidant levels in the lens and plasma decreased with age; however, this decrease could be nullified with either 1% or 2% G-Hsd treatment in a concentration- and exposure time-dependent manner. Moreover, G-Hsd treatment affected the TRPV4 distribution, but not TRPV1, AQP0, and AQP5, in the peripheral area and could maintain intracellular pressure. These findings suggest that G-Hsd has great potential as a compound to prevent presbyopia and/or cataract formation.

  • [Look Back on the Eye with Basic Research, Clinical Science and Drug Repositioning].

    Kosano H, Nakazawa Y, Hara H

    Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 141 ( 1 ) 33 - 34 2021

    ISSN  0031-6903

  • Oral consumption of α-glucosyl-hesperidin could prevent lens hardening, which causes presbyopia

    Yosuke Nakazawa, Miki Aoki, Yuri Doki, Naoki Morishita, Shin Endo, Noriaki Nagai, Megumi Funakoshi-Tago, Hiroomi Tamura

    Biochemistry and Biophysics Reports (Elsevier)  25   100885 2020.12

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2020 The Authors Presbyopia is one of the most well-known diseases of the eye, predominantly affecting the adult population after 50 years’. Due to hardening of the lens and failure of accommodative change, patients lose the ability to focus on near objects. This eye symptom is reported to be an early symptom of age-related nuclear cataract, and we have previously reported that hesperetin treatment could delay the onset of nuclear cataractogenesis induced by sodium selenite. In this study, we examined whether oral intake of α-glucosyl-hesperidin (G-Hsd), which has greater water solubility than hesperetin, could delay the onset of presbyopia. G-Hsd treatment protected lens elasticity, upregulated the mRNA expression of anti-oxidative enzymes like glutathione reductase and thioredoxin reductase 1 in the plasma and lens, and prevented premature cataract symptoms in selenite-induced cataract rat lens. Thus, the anti-presbyopic effects of G-Hsd were attributed, at least in part, to its antioxidant effects. G-Hsd represents the first oral treatment agent with anti-presbyopia and/or anti-cataract properties.

  • Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)

    Yamamoto, N., Takeda, S., Hatsusaka, N., Hiramatsu, N., Nagai, N., Deguchi, S., Nakazawa, Y., Takata, T., Kodera, S., Hirata, A., Kubo, E. and Sasaki, H.

    Cells 9 ( 12 )  2020.12

    ISSN  2073-4409

     View Summary

    The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts.

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Papers, etc., Registered in KOARA 【 Display / hide

Reviews, Commentaries, etc. 【 Display / hide

  • Look back on the eye with basic research, clinical science and drug repositioning

    Kosano H., Nakazawa Y., Hara H.

    Yakugaku Zasshi (Yakugaku Zasshi)  141 ( 1 ) 33 - 34 2021.01

    ISSN  00316903

  • フィレンシンによるアクアポリン0(AQP0)の機能解析.


    日本白内障学会誌 (日本白内障学会)  26   28-29 2014.06

    Research paper, Single Work

  • 水晶体中のRNAの解析.

    岡 美佳子, 梅澤 和寛, 中澤 洋介, 竹鼻 眞.

    日本眼科学会雑誌 (日本眼科学会)  117(10)   826-826 2013.10

    Introduction and explanation (scientific journal), Joint Work

  • 水晶体線維細胞分化に伴う遺伝子発現の変化.

    梅澤 和寛, 岡 美佳子, 中澤 洋介, 竹鼻 眞.

    日本眼科学会雑誌 (日本眼科学会)  115(9)   848-848 2011.09

    Introduction and explanation (scientific journal), Joint Work

  • 糖尿病性白内障発症前後でのアスコルビン酸トランスポーターの発現解析.

    中澤洋介, 岡美佳子, 竹鼻眞.

    日本眼科学会雑誌 (日本眼科学会)  114   809-809 2010.09

    Introduction and explanation (scientific journal), Joint Work

Presentations 【 Display / hide

  • 後発白内障におけるCapsaicinの機能解析

    杉山 裕紀、河田 沙礼、阪上 瞳子、中澤 洋介、田村 悦臣、多胡 めぐみ

    日本薬学会 第141年会, 2021.03, Oral Presentation(general)

  • 墨魚点滴 白内障予防に糖転移ヘスペリジン!?


    みなと新聞, 2020.11, Media report,etc.

  • alpha-glucosyl hesperidinの白内障予防効果の検討

    Yosuke Nakazawa

    第12回ヘスペリジン研究会, 2020.11, Oral Presentation(guest/special)

  • 水晶体透明性維持機構の解明と関連疾患予防の可能性


    日本薬学会 第140年会, 2020.03, Symposium, Workshop, Panelist (nomination)

  • Aquaporin 0a is required for water homeostasis in the zebrafish lens in vivo

    Irene Vorontsova, Alexander Vallmitjana, Yosuke Nakazawa, Belén Torrado, Thomas Schilling, James E. Hall, Enrico Gratton, Leonel S. Malacrida.

    Biophysical Society 2020, 64th Annual meeting of the Biophysical Society, 2020.02, Oral Presentation(general)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • 老眼発症機序の解明とTRPVチャネルを標的とした抗老眼薬創製の基盤研究


    文部科学省, 科学研究費補助金(文部科学省・日本学術振興会), Yosuke Nakazawa, Research grant, Principal Investigator

  • Roles for the mechanosensitive channels (TRPV1 and TRPV4) in the initiation of presb yopia and development of cataract


    JSPS-NZRS, Bilateral joint research project between Japan and New Zealand, Yosuke Nakazawa, Research grant, Principal Investigator

  • 老眼発症遅延薬の開発を見据えて:老眼発症メカニズムの解明と老眼モデル動物の開発


    Japan Health Foundation, Yosuke Nakazawa, Research grant, Principal Investigator

  • アクアポリン0およびアクアポリン5の機能解明とこれらを標的とした新規抗白内 障薬の創生


    公益財団法人 持田記念医学薬学振興財団, Research grant

  • 新規白内障治療薬の開発を目指したアクアポリン0の機能解明


    文部科学省, Grant-in-Aid for Scientific Research, Yosuke Nakazawa, Research grant, Principal Investigator

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Awards 【 Display / hide

  • 日本薬学会2019年度関東支部 奨励賞

    中澤洋介, 2019.09, 公益社団法人日本薬学会関東支部, 抗白内障薬/抗老眼薬の創製を見据えて:水晶体透明性維持機構の解明と疾患予防の基盤研究

    Type of Award: Awards of Publisher, Newspaper Company and Foundation.  Country: 日本

  • 日本白内障学会 学術賞

    中澤洋介, 2014.09, Japanese Society for Cataract Research, 水晶体のアクアポリン0の役割および機能に関する新しい知見

  • 2019年度 助成研究発表会 優秀賞

    2019.11, Japan Health Fundation

    Type of Award: Awards of National Conference, Council and Symposium

  • Imaging competion Winner 2019

    2019.07, Vector Laboratories社, lens E10 section

    Type of Award: Awards of Publisher, Newspaper Company and Foundation.  Country: CA, USA

  • Young Investigator Awards

    Yosuke Nakazawa, Rosica S. Petrova, Hiroomi Tamura, Paul J. Donaldson, 2017.12, National Foundation for Eye Research, The subcellular expression patterns of the mechano-sensitive channels TRPV1/4 in the mouse lens are modulated by changes in zonular tension

    Country: Kona, Hawaii

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Other 【 Display / hide

  • 日本薬学会 第141年会 一般シンポジウム オーガナイザー


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    日本薬学会 第141年会
    一般シンポジウム オーガナイザー

    [S20] 老化と眼疾患ーいつまでも健康な視機能をー

  • 日本薬学会 第140年会 一般シンポジウム オーガナイザー


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    日本薬学会 第140年会
    一般シンポジウム オーガナイザー

    今いちど,眼科領域の進歩を考える ―基礎,臨床,そしてドラッグリポジショニングについて―


Courses Taught 【 Display / hide











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Committee Experiences 【 Display / hide

  • 2018.07

    大会長, 第44回 水晶体研究会 

  • 2018.07

    白内障学会誌 副編集委員長, 日本白内障学会

  • 2012.06

    世話人, 水晶体研究会

  • 2012.06

    白内障学会誌 編集委員, 日本白内障学会

  • 2019.07

    評議員, 白内障学会

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