金澤 秀子 (カナザワ ヒデコ)

Kanazawa, Hideko

写真a

所属(所属キャンパス)

薬学部 薬科学科 創薬物理化学講座 (芝共立)

職名

教授

HP

外部リンク

 

研究分野 【 表示 / 非表示

  • 物理系薬学

研究キーワード 【 表示 / 非表示

  • 機能性高分子、HPLC、DDS

 

著書 【 表示 / 非表示

  • 刺激応答性高分子ハンドブック

    長瀬 健一, 金澤 秀子, 株式会社エヌ・ティー・エス, 2018年12月

    担当範囲: 温度応答性クロマトグラフィー

  • ゲルテクノロジーハンドブック、第1篇第3章第3節 機能性ナノ界面を用いた温度応答性クロマトグラフィー

    金澤 秀子, NTS, 2014年10月

    担当範囲: 80-87

  • 製剤化のサイエンス

    金澤 秀子, 第6版ネオメディカル, 2014年

  • わかりやすい薬剤学計算問題の解き方

    金澤 秀子, 改訂版ネオメディカル, 2014年

  • 図解製剤学

    金澤 秀子, 南山堂, 2013年04月

    担当範囲: 20-62

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論文 【 表示 / 非表示

  • Thermoresponsive anionic copolymer brush-grafted surfaces for cell separation

    Nagase K., Uchikawa N., Hirotani T., Akimoto A., Kanazawa H.

    Colloids and Surfaces B: Biointerfaces (Colloids and Surfaces B: Biointerfaces)  185   110565 2020年01月

    ISSN  09277765

     概要を見る

    © 2019 Elsevier B.V. Cell separation methods that do not require surface modification of cells are needed in tissue engineering and regenerative medicine. We developed thermoresponsive anionic polymer brushes for cell separation without modification of the cell surfaces. Copolymer brush poly(N-isopropylacrylamide-co-N-tert-butylacrylamide-co-tert-butyl acrylate, P(NIPAAm-co-tBAAm-co-tBA), was prepared on a cover glass plate through activator regenerated by electron transfer atom transfer radical polymerization (ARGET-ATRP). The tert-butyl group of the copolymer brush was then deprotected and a P(NIPAAm-co-tBAAm-co-acrylic acid (AAc)) brush-modified glass surface was obtained. ARGET-ATRP synthesis achieved polymers with low polydispersity. The negative surface charge of the polymer brush-modified substrates was evaluated using zeta potential measurements and the phase transition temperature of the polymer was modulated between 37–20 °C to perform cell adhesion and detachment, respectively. The adhesion and detachment behavior of cells used in cardiovascular tissue engineering on the thermoresponsive anionic polymer brushes was investigated. Normal human umbilical vein endothelial cells (HUVEC) exhibited prompt detachment from the thermoresponsive anionic polymer brush surfaces. In addition, normal human aortic smooth muscle cells (SMC) exhibited relatively high adhesion on thermoresponsive anionic polymer brush-modified surfaces compared with those modified with thermoresponsive polymer brushes without anionic groups. By utilizing the difference in the cell adhesion and detachment properties of the cell types, a mixture of HUVEC and SMC was separated simply by altering the applied temperature. This result indicated that the prepared thermoresponsive anionic polymer brush-modified glass surface could be used as a tool for the separation of cells in cardiovascular tissue engineering.

  • Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins

    Nakao M., Inanaga D., Nagase K., Kanazawa H.

    Regenerative Therapy (Regenerative Therapy)  11   34 - 40 2019年12月

     概要を見る

    © 2019 The Japanese Society for Regenerative Medicine Introduction: Stem cell therapy with mesenchymal stem cells (MSCs)has been widely used in many clinical trials, and therapy with MSC sheets shows promise for patients. However, there are few reports characterizing MSC sheets. In the present study, the properties of MSC sheets derived from bone marrow, adipose tissue, and umbilical cord were evaluated. Methods: Cell sheets were fabricated with MSCs from different tissue origins in temperature-responsive cell culture dishes with and without pre-coating of fetal bovine serum (FBS). MSC adhesion behavior in the culture dish was observed. Secretion of cytokines related to cell proliferation and immune regulation from MSC sheets was investigated by ELISA. The adhesion properties of the MSC sheets were investigated by time-lapse microscopy. Results: Different cell adhesion and proliferation rates in temperature-responsive cell culture dishes were observed among the three types of MSCs. FBS pre-coating of the dishes enhanced cell attachment and proliferation in all cell types. Harvested cell sheets showed high attachment capacity to tissue culture polystyrene dish surfaces. Conclusions: MSC sheets can be fabricated from MSCs from different tissue origins using temperature-responsive cell culture dishes. The fabricated MSC sheets could be useful in cell transplantation therapies by choosing appropriate types of MSCs that secrete therapeutic cytokines for the targeted diseases.

  • Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets.

    Nakao M, Kim K, Nagase K, Grainger DW, Kanazawa H, Okano T

    Stem cell research & therapy 10 ( 1 ) 353 2019年11月

  • 効果的な心筋細胞シート移植のためのVEGF徐放ファイバーマットの設計

    長瀬 健一,金澤 秀子

    Drug Delivery System 34 ( 4 ) 173 - 178 2019年07月

    研究論文(学術雑誌), 共著, 査読有り

  • Temperature-modulated cell-separation column using temperature-responsive cationic copolymer hydrogel-modified silica beads

    Nagase K., Inanaga D., Ichikawa D., Mizutani Akimoto A., Hattori Y., Kanazawa H.

    Colloids and Surfaces B: Biointerfaces (Colloids and Surfaces B: Biointerfaces)  178   253 - 262 2019年06月

    ISSN  09277765

     概要を見る

    © 2019 Elsevier B.V. There is strong demand for cell separation methods that do not decrease cell activity or modify cell surfaces. Here, new temperature-modulated cell-separation columns not requiring cell-surface premodification are described. The columns were packed with temperature-responsive cationic polymer hydrogel-modified silica beads. Poly(N-isopropylacrylamide-co-n-butyl methacrylate-co-N,N-dimethylaminopropyl acrylamide) hydrogels with various cationic moieties were attached to silica-bead surfaces by radical polymerization using N,Nʹ-methylenebisacrylamide as a crosslinking agent. The beads were packed into solid-phase extraction columns, and temperature-dependent cell elution from the columns was found using HL-60 and Jurkat cells. The retention HL-60 and Jurkat cells in columns containing cationic beads at 37 °C was 95.3% to 99.6% and 95.0% to 98.8%, respectively. By contrast, beads without cationic properties exhibited low cell retention (20.6% for HL-60 and 32.5% for Jurkat cells). The cells were mainly retained through both electrostatic and hydrophobic interactions. The retained HL-60 (4.9%) and Jurkat cells (40%) were eluted at 4 °C from the column with a low composition of cationic monomer (DMAPAAm, 1 mol% in copolymer), because the temperature-responsive hydrogels on the beads became hydrophilic, decreasing the hydrophobic interactions between the cells and the beads. A higher number of Jurkat cells than HL-60 cells were eluted because of differences in their electrostatic properties (Jurkat cells: −2.53 mV; HL-60 cells: −20.7 mV). The results indicated that cell retention by the hydrogel-coated beads packed in a solid phase extraction column could be modulated simply by changing the temperature.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

総説・解説等 【 表示 / 非表示

  • Comparison of plasma propofol concentration for apnea, response to mechanical ventilation, and airway device between endotracheal tube and supraglottic airway device in beagles

    Iizuka T., Masui K., Kanazawa H., Nishimura R.

    Journal of Veterinary Medical Science (Journal of Veterinary Medical Science)  80 ( 9 ) 1420 - 1423 2018年09月

    ISSN  09167250

     概要を見る

    © 2018 The Japanese Society of Veterinary Science. The relationships between propofol plasma concentrations and the pharmacodynamic endpoints may differ according to a type of airway device. To clarify these relationships in different airway devices would be useful to avoid the complication such as apnea and intraoperative awareness. The aim of this study was to investigate the influence of difference of airway device on propofol requirement during maintenance of anesthesia in dogs. We compared the influence of airway devices on the plasma propofol concentrations for apnea, response to mechanical ventilation, and response to airway device between endotracheal tube (ETT) and supraglottic airway device (SGAD) in Beagles. The pharmacodynamic effects were repeatedly assessed at varying propofol concentrations. The plasma concentrations (mean ± SD) of propofol in the ETT and SGAD groups were 10.2 ± 1.8 and 10.9 ± 2.4 µg/ml for apnea (P=0.438), 7.9 ± 1.2 and 7.4 ± 1.5 µg/ml for response to mechanical ventilation (P=0.268), and 5.2 ± 0.7 and 5.4 ± 1.5 µg/ml for response to airway device (P=0.580), respectively. Required propofol concentration during maintenance of anesthesia may be similar between ETT and SGAD. Without moderate to strong stimuli such as airway device insertion or painful stimulation during surgery, the type of airway device may have little impact on required propofol concentration during maintenance of anesthesia in dogs.

  • 物理化学的視点から薬剤師業務を考える

    金澤 秀子

    ファルマシア 53 ( 4 ) 301 - 304 2017年

    総説・解説(学術雑誌), 単著

  • ビタミンE含有複合型サプリメントおよび成分併用の機能評価

    永田 佳子, 平出 園絵, 浜崎 真衣, 奥住 彩香, 金澤 秀子

    ビタミンE研究の進歩 (ビタミンE研究会)  17   142 - 148 2016年12月

    総説・解説(その他), 共著

  • 複合型サプリメントの抗酸化機能評価

    永田 佳子,平出 園絵,星野 由依,重田 まりあ,金澤 秀子

    分析化学 65 ( 9 ) 519 - 526 2016年

    速報,短報,研究ノート等(学術雑誌), 共著

  • 物理化学的視点で目指す医療への貢献

    金澤 秀子

    薬剤学 75 ( 3 ) 154 - 159 2015年

    総説・解説(学術雑誌), 単著

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研究発表 【 表示 / 非表示

  • 正電荷を有する温度応答性高分子を充填剤に用いた幹細胞分離カラムの開発

    枝常吾郎,山田創太,長瀬健一,金澤秀子

    第17回 次世代を担う若手のためのフィジカル・ファーマフォーラム (滋賀県大津市) , 2019年09月, 口頭(一般)

  • 機能性高分子修飾リポソームを用いた温度依存的核酸デリバリーに関する検討

    梯尚貴,綾野絵理,山田創太,長瀬健一,米谷芳枝,金澤秀子

    第17回 次世代を担う若手のためのフィジカル・ファーマフォーラム (滋賀県大津市) , 2019年09月, 口頭(一般)

  • 温度応答性カチオン交換型ミックスモードカラムの開発

    長瀬 健一、 渡辺 真梨亜、善 文比古、金澤 秀子

    第32回 バイオメディカル分析科学シンポジウム (東京) , 2019年08月, 口頭(一般)

  • 温度応答性アニオン交換クロマトグラフィーによる核酸の分離精製

    山崎 開智、前川 祐太朗、井原 美和、長瀬 健一、金澤 秀子

    第32回 バイオメディカル分析科学シンポジウム (東京) , 2019年08月, ポスター(一般)

  • セラノスティクス応用に向けたアミノ酸トランスポーター標的型温度応答性薬物送達キャリアの開発

    藤條 惠、松浦 みなみ、長瀬 健一、金澤 秀子

    第32回 バイオメディカル分析科学シンポジウム (東京) , 2019年08月, 口頭(一般)

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競争的資金等の研究課題 【 表示 / 非表示

  • 細胞治療・バイオ創薬のための高機能分離精製技術の開発

    2016年04月
    -
    2019年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 金澤 秀子, 基盤研究(B), 補助金,  代表

受賞 【 表示 / 非表示

  • 日本分析化学会 学会賞

    金澤 秀子, 2017年09月, 日本分析化学会, 温度応答性クロマトグラフィーを用いた分離・分析システムの創製とその応用

    受賞区分: 国内学会・会議・シンポジウム等の賞,  受賞国: 日本

  • クロマトグラフィー科学会学会賞

    ○金澤秀子., 2008年12月, クロマトグラフィー科学会, 機能性高分子を用いた温度制御型新規分離システムの開発.

  • HPLC 2005 Best Poster Paper Award First Prize.

    Kanazawa H*, Okada Y, Ayano E, Matsushima Y, Okano T., 2005年07月, 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2005)

    受賞区分: 国内外の国際的学術賞

  • 日本分析化学会関東支部新世紀賞.

    金澤秀子., 2002年03月, 日本分析化学会関東支部

    受賞区分: 国内学会・会議・シンポジウム等の賞

  • 生命科学啓明賞.

    金澤秀子., 2002年03月, 財団法人 啓明会

    受賞区分: 出版社・新聞社・財団等の賞

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担当授業科目 【 表示 / 非表示

  • 課題研究(創薬物理化学)

    2019年度

  • 演習(創薬物理化学)

    2019年度

  • 卒業研究C

    2019年度

  • 卒業研究B

    2019年度

  • 卒業研究A

    2019年度

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