Kanazawa, Hideko

写真a

Affiliation

Faculty of Pharmacy 創薬分析化学講座 (Mita)

Position

Professor Emeritus

Related Websites

External Links
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Research Areas 【 Display / hide 】

  • Life Science / Pharmaceutical analytical chemistry and physicochemistry

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Research Keywords 【 Display / hide 】

  • Functional Polymer, HPLC, DDS

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Books 【 Display / hide 】

  • 刺激応答性高分子ハンドブック

    長瀬 健一, 金澤 秀子, 株式会社エヌ・ティー・エス, 2018.12

    Scope: 温度応答性クロマトグラフィー

  • ゲルテクノロジーハンドブック、第1篇第3章第3節 機能性ナノ界面を用いた温度応答性クロマトグラフィー

    KANAZAWA Hideko, NTS, 2014.10

    Scope: 80-87

  • 製剤化のサイエンス

    KANAZAWA Hideko, 第6版ネオメディカル, 2014

  • わかりやすい薬剤学計算問題の解き方

    KANAZAWA Hideko, 改訂版ネオメディカル, 2014

  • 図解製剤学

    KANAZAWA Hideko, 南山堂, 2013.04

    Scope: 20-62

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Papers 【 Display / hide 】

  • Temperature-modulated interactions between thermoresponsive strong cationic copolymer-brush-grafted silica beads and biomolecules

    Nagase K., Suzuki S., Kanazawa H.

    Heliyon 10 ( 15 ) e34668 2024.08

    ISSN  24058440

    　View Summary

    Thermoresponsive polymer brushes have attracted considerable research attention owing to their unique properties. Herein, we developed silica beads grafted with poly(N-isopropylacrylamide (NIPAAm)-co-3-acrylamidopropyl trimethylammonium chloride (APTAC)-co-tert-butyl acrylamide (tBAAm) and P(NIPAAm-co-APTAC-co-n-butyl methacrylate(nBMA)) brushes. The carbon, hydrogen, and nitrogen elemental analysis of the copolymer-grated silica beads revealed the presence of a large amount of the grafted copolymer on the silica beads. The electrostatic and hydrophobic interactions between biomolecules and prepared copolymer brushes were analyzed by observing their elution behaviors via high-performance liquid chromatography using the copolymer-brush-modified beads as the stationary phase. Adenosine nucleotides were retained in the bead-packed columns, which was attributed to the electrostatic interaction between the copolymers and adenosine nucleotides. Insulin was adsorbed on the copolymer brushes at high temperatures, which was attributed to its electrostatic and hydrophobic interactions with the copolymer. Similar adsorption behavior was observed in case of albumin. Further, at a low concentration of the phosphate buffer solution, albumin was adsorbed onto the copolymer brushes even at relatively low temperatures owing to its enhanced electrostatic interaction with the copolymer. These results indicated that the developed thermoresponsive strong cationic copolymer brushes can interact with peptides and proteins through a combination of electrostatic and temperature-modulated hydrophobic interactions. Thus, the developed copolymer brushes exhibits substantial potential for application in chromatographic matrices for the analysis and purification of peptides and proteins.

  • Thermoresponsive mixed polymer brush to effectively control the adhesion and separation of stem cells by altering temperature

    Nagase K., Wakayama H., Matsuda J., Kojima N., Kanazawa H.

    Materials Today Bio 20   100627 2023.06

    　View Summary

    During the last few decades, thermoresponsive materials for modulating cell adhesion have been investigated for the application of tissue engineering. In this study, we developed thermoresponsive mixed polymer brushes consisting of poly(N-isopropylacrylamide) (PNIPAAm) and poly(N,N-dimethylaminopropylacrylamide) (PDMAPAAm). The mixed polymer brushes were prepared on a glass substrate via the reversible addition-fragmentation chain transfer polymerization of DMAPAAm and subsequent atom transfer radical polymerization of NIPAAm. The mixed polymer brushes grafted to glass exhibited increased cationic properties by increasing the grafted PDMAPAAm length. The shrinking and extension of PNIPAAm exposed and concealed PDMAPAAm, respectively, indicating that the surface cationic properties can be controlled by changing the temperature. At 37 ​°C, the prepared mixed polymer brushes enhanced cell adhesion through their electrostatic interactions with cells. They also exhibited various thermoresponsive adhesion and detachment properties using various types of cells, such as mesenchymal stem cells. Temperature-controlled cell adhesion and detachment behavior differed between cell types. Using the prepared mixed polymer brush, we separated MSCs from adipocytes and HeLa cells by simply changing the temperature. Thus, the thermoresponsive mixed polymer brushes may be used to separate mesenchymal stem cells from their differentiated or contaminant cells by altering the temperature.

  • Real-time in situ X-ray micro-computed tomography study of the effect of impurities on the crystallization of amorphous nifedipine

    Amano Y., Misawa T., Miyazaki T., Ando D., Koide T., Izutsu K.i., Kanazawa H., Hanaoka K., Yamamoto E.

    Journal of Pharmaceutical and Biomedical Analysis 226   115248 2023.03

    ISSN  07317085

    　View Summary

    Controlling the physical stability of noncrystalline active pharmaceutical ingredients remains a major challenge in the development of amorphous formulations such as amorphous solid-dispersion (ASD) formulations. To establish new evaluation and formulation strategies, the spatial distribution of the crystal phase in bulk amorphous nifedipine (NFD) was investigated as a model. The crystallization of amorphous NFD and the effect of a deliberately added impurity were investigated using powder X-ray diffraction (PXRD), differential scanning calorimetry and real-time in situ X-ray micro-computed tomography (X-ray CT). The stability data of amorphous samples, i.e., NFD and a mixture of NFD with an oxidative degradation product of NFD, impurity A (Imp A), at a weight ratio of 90:10, presented as percent amorphous remaining, suggests that Imp A accelerates the bulk crystal growth of NFD. Real-time in situ X-ray CT results showed surface-enhanced crystal growth and cavity formation in solid NFD samples. Moreover, the crystals were heterogeneous in density. These results suggest that Imp A affects the physical stability of the amorphous NFD. X-ray CT equipped with a heating unit can aid in-situ evaluation and assessment of physicochemical properties and physical stability of amorphous samples and formulations.

  • Thermoresponsive bio-affinity interfaces for temperature-modulated selective capture and release of targeted exosomes

    Nagase K., Yamazaki K., Maekawa Y., Kanazawa H.

    Materials Today Bio 18   100521 2023.02

    　View Summary

    The existing methods for exosome isolation, such as ultracentrifugation, size exclusion, and affinity separation, suffer from some limitations. Herein, we aimed to develop temperature-modulated exosome-capturing materials using thermoresponsive polymers and peptides with affinity for exosomes. Poly(2-hydroxyethyl methacrylate-co-propargyl acrylate)-b-poly(N-isopropylacrylamide) (P(HEMA-co-PgA)-b-PNIPAAm) was grafted on silica beads via a two-step process of activator regenerated by electron transfer atom transfer radical polymerization. Peptides with affinity for exosomes were conjugated to the propargyl group of the bottom P(HEMA-co-PgA) segment of the copolymer via a click reaction. The prepared copolymer-grafted beads were characterized by elemental analysis, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, gel permeation chromatography, and the turbidity of the polymer solution. Results indicated that the copolymer and peptide were successfully modified on the silica beads. Exosomes from SK-BR-3 ​cells, a human breast cancer cell line, were selectively captured on the prepared beads at 37 ​°C, as the upper PNIPAAm segment shrank and the affinity between the peptide and exosome was enhanced. Upon lowering the temperature to 4 ​°C, the captured exosomes were released from the copolymer brush because of the extension of the PNIPAAm segment that reduced the affinity between peptides and exosomes. These findings demonstrated that the prepared copolymer brush-grafted silica beads can capture and release targeted exosomes via temperature modulation. Taken together, the developed copolymer brush-grafted silica beads would be useful for the separation of exosomes using simple procedures such as temperature modulation.

  • A thermoresponsive cationic block copolymer brush-grafted silica bead interface for temperature-modulated separation of adipose-derived stem cells

    Nagase K., Okada A., Matsuda J., Ichikawa D., Hattori Y., Kanazawa H.

    Colloids and Surfaces B: Biointerfaces 220   112928 2022.12

    ISSN  09277765

    　View Summary

    Adipose-derived mesenchymal stem cells (ADSCs) have beneficial effects in cell transplantation therapy; these cells are collected from adipose tissue using low-invasive methods. However, to prepare ADSCs for cell therapy, a cell separation method that neither involves modification of the cell surface nor causes loss of cell activity is needed. Here, we aimed to develop ADSC separation columns using thermoresponsive cationic block copolymer brush-grafted beads as packing materials. The block copolymer brush was formed by a bottom cationic segment, poly(N,N-dimethylaminopropylacrylamide) (PDMAPAAm), and an upper thermoresponsive segment, poly(N-isopropylacrylamide) (PNIPAAm), and was grafted in two atom transfer radical polymerization reactions. The copolymer brush-grafted silica beads were packed into a column. An ADSC suspension was introduced into the columns at 37 °C and adsorbed on the copolymer brush-modified beads through electrostatic and hydrophobic interactions with the PDMAPAAm and PNIPAAm segments, respectively. The adsorbed ADSCs eluted from the column by lowering the temperature to 4 °C. In contrast, most Jurkat and vascular endothelial cells eluted at 37 °C, because of the relatively weaker electrostatic interactions with the block copolymer brush compared to ADSCs. Using the prepared column, a mixture of ADSCs and Jurkat cells was separated by changing the column temperature. The recovered ADSCs exhibited cell activity. The developed cell separation column may be useful for isolating ADSCs without cell surface modification, while maintaining cell activity.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 細胞導入を制御可能な刺激応答性高分子を用いたナノキャリアの開発

    山之内 翔, 勝山 直哉, 蛭田 勇樹, 綾野 絵理, 金澤 秀子

    高分子論文集 75 ( 2 ) 116 - 127 2918.03

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • Temperature-responsive chromatography for bioseparations

    Kenichi Nagase, Hideko Kanazawa

    .Analytica Chimica Acta 1138   191 - 212 2020.11

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • 効果的な心筋細胞シート移植のためのVEGF徐放ファイバーマットの開発

    長瀬健一, 金澤秀子

    Drug Delivery System (じほう)  34 ( 3 ) 173 - 178 2019.07

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work,  ISSN  0913-5006

  • 効果的ながん治療を目指したCD44ターゲティングナノキャリア

    山之内 翔, 金澤 秀子

    Drug Delivery System (じほう)  34 ( 1 ) 38 - 45 2019.01

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work,  ISSN  09135006

  • Comparison of plasma propofol concentration for apnea, response to mechanical ventilation, and airway device between endotracheal tube and supraglottic airway device in beagles

    Iizuka T., Masui K., Kanazawa H., Nishimura R.

    Journal of Veterinary Medical Science (Journal of Veterinary Medical Science)  80 ( 9 ) 1420 - 1423 2018.09

    ISSN  09167250

    　View Summary

    © 2018 The Japanese Society of Veterinary Science. The relationships between propofol plasma concentrations and the pharmacodynamic endpoints may differ according to a type of airway device. To clarify these relationships in different airway devices would be useful to avoid the complication such as apnea and intraoperative awareness. The aim of this study was to investigate the influence of difference of airway device on propofol requirement during maintenance of anesthesia in dogs. We compared the influence of airway devices on the plasma propofol concentrations for apnea, response to mechanical ventilation, and response to airway device between endotracheal tube (ETT) and supraglottic airway device (SGAD) in Beagles. The pharmacodynamic effects were repeatedly assessed at varying propofol concentrations. The plasma concentrations (mean ± SD) of propofol in the ETT and SGAD groups were 10.2 ± 1.8 and 10.9 ± 2.4 µg/ml for apnea (P=0.438), 7.9 ± 1.2 and 7.4 ± 1.5 µg/ml for response to mechanical ventilation (P=0.268), and 5.2 ± 0.7 and 5.4 ± 1.5 µg/ml for response to airway device (P=0.580), respectively. Required propofol concentration during maintenance of anesthesia may be similar between ETT and SGAD. Without moderate to strong stimuli such as airway device insertion or painful stimulation during surgery, the type of airway device may have little impact on required propofol concentration during maintenance of anesthesia in dogs.

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Presentations 【 Display / hide 】

  • 温度応答性培養基材を用いたラベルフリー肝細胞分離法の開発

    小島直人,長瀬健一,赤池敏宏,後藤光昭,金澤秀子

    第64回 日本薬学会 関東支部, 

    2020.09
    , 

    Poster presentation

  • 機能性高分子を用いた温度制御型タンパク質分離法の開発

    北澤 早紀子， 山田 創太， 長瀬 健一， 金澤 秀子

    日本分析化学会 第69年会, 

    2020.09
    , 

    Poster presentation

  • 正電荷を有する温度応答性充填剤を用いたラベルフリー細胞分離法の開発

    枝常 吾郎, 山田 創太, 長瀬 健一, 金澤 秀子

    日本分析化学会 第69年会, 

    2020.09
    , 

    Poster presentation

  • オリゴヌクレオチド分離のための温度応答性アニオン交換クロマトグラフィーの開発

    山崎 開智, 前川 祐太朗, 井原 美和, 長瀬 健一, 金澤 秀子

    日本分析化学会 第69年会, 

    2020.09
    , 

    Poster presentation

  • 温度応答性－カチオン性高分子ブラシとタンパク質・細胞との相互作用制御

    長瀬健一，金澤 秀子

    第69回高分子討論会, 

    2020.09
    , 

    Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 再生医療を革新的に効率化する機能性バイオ界面の創製

    2019.04
    -
    2023.03

    文部科学省・日本学術振興会, 科学研究費助成事業, 基盤研究(B), Coinvestigator(s)

  • 酸素産生ナノ粒子を用いた革新的細胞組織移植法の確立

    2018.04
    -
    2021.03

    文部科学省・日本学術振興会, 科学研究費助成事業, 挑戦的研究(萌芽), Coinvestigator(s)

  • Development of high performance separation and purification technology for cell therapy and biopharmaceuticals

    2016.04
    -
    2019.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

  • 疾患バイオマーカー探索のための機能的RNA-タンパク複合体分離精製法の構築

    2013.04
    -
    2015.03

    文部科学省・日本学術振興会, 科学研究費助成事業, 基盤研究(B), Principal investigator

  • 生体内動態の時空間制御による機能的核酸デリバリーシステムの創製

    2013.04
    -
    2014.03

    文部科学省・日本学術振興会, 科学研究費助成事業, 挑戦的萌芽研究, Principal investigator

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Awards 【 Display / hide 】

  • 日本分析化学会 学会賞

    金澤　秀子, 2017.09, 日本分析化学会, 温度応答性クロマトグラフィーを用いた分離・分析システムの創製とその応用

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • クロマトグラフィー科学会学会賞

    金澤秀子., 2008.12, クロマトグラフィー科学会, 機能性高分子を用いた温度制御型新規分離システムの開発.

  • HPLC 2005 Best Poster Paper Award First Prize.

    Hideko Kanazawa, 2005.07, 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2005)

    Type of Award： International academic award (Japan or overseas)

  • 日本分析化学会関東支部新世紀賞

    金澤秀子., 2002.03, 日本分析化学会関東支部

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • 生命科学啓明賞

    金澤秀子., 2002.03, 財団法人　啓明会

    Type of Award： Award from publisher, newspaper, foundation, etc.

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Courses Taught 【 Display / hide 】

  • DOCTORAL LECTURE ON CHEMICAL PHARMACY 1

    2021

  • ADVANCED DRUG DELIVERY AND PHARMACOKINETICS

    2021

  • FUTURE MEDICINE : REGENERATIVE MEDICINE AND DRUG DELIVERY SYSTEM

    2020

  • ENGLISH EXERCISES FOR PHARMACEUTICAL SCIENCES

    2020

  • EARLY EXPOSURE TO INDUSTRY

    2020

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