Papers - Suzuki, Sayo

S100A10 protein expression is associated with oxaliplatin sensitivity in human colorectal cancer cells.

Suzuki S, Yamayoshi Y, Nishimuta A, Tanigawara Y*.

Proteome Science (BioMed Central)  9   76 2011.12

Research paper (scientific journal), Joint Work, Lead author, Accepted

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Background: Individual responses to oxaliplatin (L-OHP)-based chemotherapy remain unpredictable. The objective of our study was to find candidate protein markers for tumor sensitivity to L-OHP from intracellular proteins of human colorectal cancer (CRC) cell lines. We performed expression difference mapping (EDM) analysis of whole cell lysates from 11 human CRC cell lines with different sensitivities to L-OHP by using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), and identified a candidate protein by liquid chromatography/mass spectrometry ion trap time-of-flight (LCMS-IT-TOF).Results: Of the qualified mass peaks obtained by EDM analysis, 41 proteins were differentially expressed in 11 human colorectal cancer cell lines. Among these proteins, the peak intensity of 11.1 kDa protein was strongly correlated with the L-OHP sensitivity (50% inhibitory concentrations) (P < 0.001, R 2 = 0.80). We identified this protein as Protein S100-A10 (S100A10) by MS/MS ion search using LCMS-IT-TOF. We verified its differential expression and the correlation between S100A10 protein expression levels in drug-untreated CRC cells and their L-OHP sensitivities by Western blot analyses. In addition, S100A10 protein expression levels were not correlated with sensitivity to 5-fluorouracil, suggesting that S100A10 is more specific to L-OHP than to 5-fluorouracil in CRC cells. S100A10 was detected in cell culture supernatant, suggesting secretion out of cells.Conclusions: By proteomic approaches including SELDI technology, we have demonstrated that intracellular S100A10 protein expression levels in drug-untreated CRC cells differ according to cell lines and are significantly correlated with sensitivity of CRC cells to L-OHP exposure. Our findings provide a new clue to searching predictive markers of the response to L-OHP, suggesting that S100A10 is expected to be one of the candidate protein markers. © 2011 Suzuki et al; licensee BioMed Central Ltd.

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患者指導用資材を活用した実践的な吸入指導実習の構築.

寺島朝子*, 前澤佳代子, 高橋麻由, 竹村知子, 小林典子, 大塚尚子, 鈴木 小夜, 門田佳子, 木津純子.

日病薬誌 (日本病院薬剤師会)  47 ( 3 ) 323 - 328 2011.03

Research paper (scientific journal), Joint Work, Accepted

Protein S100A10: Expression and correlation with sensitivity to oxaliplatin in colorectal cancer cells.

Suzuki S, Nishimuta A, Tanigawara Y.

Proc Annu Meet Am Assoc Cancer Res (American Association for Cancer Research)  51   870 2010.04

Research paper (international conference proceedings), Joint Work, Lead author, Accepted

S100A10 as a potential protein biomarker for oxaliplatin sensitivity in colorectal cancer cells.

Suzuki S, Yamayoshi Y, Nishimuta A, Watanabe M, Tanigawara Y.

Proc Annu Meet Am Assoc Cancer Res (American Association for Cancer Research)  50   699 2009.04

Research paper (international conference proceedings), Joint Work, Lead author, Accepted

Protein biomarkers for predicting sensitivity to Oxaliplatin by SELDI-TOF MS screening.

Suzuki S, Yamayoshi Y., Nishimuta A., Watanabe M., Tanigawara Y.

Proceedings of the CBI Society Annual Meeting 2008 (International Synposium). (The Chemo-Bio Informatics (CBI) Society)     144 2008.10

Research paper (international conference proceedings), Joint Work, Lead author, Accepted

Discovery of blood biomarkers to predict sensitivity to CPT-11 by metabolome analysis.

Watanabe M, Arita E, Nishimuta A, Suzuki T , Yamayoshi Y, Suzuki S, Iketani O, Sagawa H, Ishikawa T, Matsuzaki T, Soga T, Tanigawara Y.

Proceedings of the CBI Society Annual Meeting 2008 (International Synposium). (The Chemo-Bio Informatics (CBI) Society)     142 2008.10

Research paper (international conference proceedings), Joint Work, Accepted

CE-TOFMS metabolome analysis of intracellular metabolite kinetics after 5-FU exposure.

Nishimuta A, Yamayoshi Y, Hirayama A, Abe J, Nagashima A, Sato H, Suzuki S, Watanabe M, Soga T, Tanigawara Y.

Proceedings of the CBI Society Annual Meeting 2008 (International Synposium). (The Chemo-Bio Informatics (CBI) Society)     143 2008.10

Research paper (international conference proceedings), Joint Work, Accepted

Metabolomic screening for blood biomarkers to predict sensitivity to CPT-11 by using CE-TOFMS.

Arita E, Watanabe M, Yamayoshi Y, Nishimuta A, Suzuki S, Iketani O, Sagawa H, Ishikawa T, Matsuzaki T, Soga T, Tanigawara Y.

Proc Annu Meet Am Assoc Cancer Res (American Association for Cancer Research)  49   1139 2008.04

Research paper (international conference proceedings), Joint Work, Accepted

Proteomic approach to discover new biomarkers that can predict Oxaliplatin sensitivity by using SELDI-TOF MS.

Suzuki S, Yamayoshi Y, Nishimuta A, Watanabe M, Tanigawara Y.

Proc Annu Meet Am Assoc Cancer Res (American Association for Cancer Research)  49   1140 2008.04

Research paper (international conference proceedings), Joint Work, Lead author, Accepted

Intracellular metabolite kinetics after 5-FU exposure by CE-TOFMS metabolome analysis.

Nishimuta A, Yamayoshi Y, Hirayama A, Abe J, Nagashima A, Sato H, Suzuki S, Watanabe M, Soga T, Tanigawara Y.

Proc Annu Meet Am Assoc Cancer Res (American Association for Cancer Research)  49   608 2008.04

Research paper (international conference proceedings), Joint Work, Accepted