Kimura, Masaki

写真a

Affiliation

Faculty of Pharmacy, Department of Pharmacy 薬物治療学講座 (Shiba-Kyoritsu)

Position

Assistant Professor/Senior Assistant Professor

External Links

Academic Background 【 Display / hide

  • 2001.03

    Waseda University, Graduate School, Division of Human Science

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(人間科学), Waseda University, Coursework, 2001.01

 

Research Areas 【 Display / hide

  • General physiology

  • Applied health science (Applied Health Sciences)

  • Sports science (Sports Science)

  • Environmental physiology (including physical medicine and nutritional physiology) (Environmental Physiology(includes Sports Medicine/Nutritional Physiology))

  • Pathological medical chemistry

Research Keywords 【 Display / hide

  • Obesity

  • Senescence

  • Anti-Aging

  • Exercise therapy

  • Dietary therapy

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Books 【 Display / hide

  • Advanced Glycation End-products (AGEs)阻害薬がOLEFTラットの肝および脂肪組織に及ぼす影響. 「酸化ストレスと肝疾患 8巻」.

    木村真規、南雲まい、飯田陽子、山田華名、齋藤義正、齋藤英胤., 株式会社嵯峨野, 東京, 2012.09

    Scope: pp67-72

  • 14. 非ステロイド系抗炎症薬. 杉崎徹三編.「臨床家のための腎毒性物質のすべて」.

    木村真規, 柴崎敏昭., シュプリンガー・ジャパン, 東京, 2008.06

    Scope: 227-248

  • 51. 前立腺肥大症 6 処方薬の解説. 富野康日己, 望月正隆編.「薬学セレクト 疾患と薬物治療-知っておきたいcommon disease 101-」.

    木村真規., 医歯薬出版, 東京, 2008.05

    Scope: 228-230

  • 52. 前立腺癌 6 処方薬の解説. 富野康日己, 望月正隆編.「薬学セレクト 疾患と薬物治療-知っておきたいcommon disease 101-」.

    木村真規., 医歯薬出版, 東京, 2008.05

    Scope: 233-234

  • 96. 骨軟化症 6 処方薬の解説. 富野康日己, 望月正隆編.「薬学セレクト 疾患と薬物治療-知っておきたいcommon disease 101-」.

    木村真規., 医歯薬出版, 東京, 2008.05

    Scope: 407-407

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Papers 【 Display / hide

  • The Effects of Continuous and Withdrawal Voluntary Wheel Running Exercise on the Expression of Senescence-Related Genes in the Visceral Adipose Tissue of Young Mice.

    Masaki Kimura, Seiya Suzuki, Atsushi Moriya, Kazuki Nogami, Ryoei Uchida, Yoshimasa Saito, Hidetsugu Saito

    Int. J. Mol. Sci. 2021, 22(1), 264 22 ( 1 ) 264 2020.12

    Research paper (scientific journal), Joint Work, Accepted

  • Glucose Depletion Enhances the Stem Cell Phenotype and Gemcitabine Resistance of Cholangiocarcinoma Organoids through AKT Phosphorylation and Reactive Oxygen Species.

    Yoshikawa N, Saito Y, Manabe H, Nakaoka T, Uchida R, Furukawa R, Muramatsu T, Sugiyama Y, Kimura M, Saito H.

    Cancers (Basel). (Cancers)  11 ( 12 ) e1993 2019.12

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Cancer cells are strongly dependent on the glycolytic pathway for generation of energy even under aerobic condition through a phenomenon known as the Warburg effect. Rapid proliferation of cancer cells is often accompanied by high glucose consumption and abnormal angiogenesis, which may lead to glucose depletion. In the present study, we investigated how cholangiocarcinoma cells adapt to glucose depletion using a 3D organoid culture system. We cultured organoids derived from cholangiocarcinoma under glucose-free condition and investigated cell proliferation, expression of stem cell markers and resistance to gemcitabine. Cholangiocarcinoma organoids cultured under glucose-free condition showed reduced proliferation but were able to survive. We also observed an increase in the expression of stem cell markers including LGR5 and enhancement of stem cell phenotypic characteristics such as resistance to gemcitabine through AKT phosphorylation and reactive oxygen species. These findings indicate that cholangiocarcinoma cells are able to adapt to glucose depletion through enhancement of their stem cell phenotype in response to changes in microenvironmental conditions.

  • Effects of combined therapy of ACE inhibitor and exercise on cardiovascular functions and morphology of the heart and kidneys in SHR.

    Masato Suzuki, Noriko Hozumi, Hidefumi Waki, Masaki Kimura, Tetsuya Seino, Naoko Onuma, Daisuke Shindo.

    J. Phys. Fitness Sports Med. 8 ( 5 ) 229 - 240 2019.08

    Research paper (scientific journal), Joint Work, Accepted

  • Coffee brew intake can prevent the reduction of lens glutathione and ascorbic acid levels in hfd-fed animals

    Nakazawa Y., Ishimori N., Oguchi J., Nagai N., Kimura M., Funakoshi-Tago M., Tamura H.

    Experimental and Therapeutic Medicine (Experimental and Therapeutic Medicine)  17 ( 2 ) 1420 - 1425 2019.02

    ISSN  17920981

     View Summary

    © 2018, Spandidos Publications. All rights reserved. The lens has high concentrations of glutathione (GSH) and ascorbic acid (AsA) to maintain redox activity and prevent cataract formation, which is the leading cause of visual impairment worldwide. Metabolic syndrome is reported to be linked with a higher risk of age-associated cataract. As it was demonstrated previously that coffee consumption improved high-fat diet (HFD) -induced metabolic symptoms, it was hypothesized that coffee intake could delay the onset of obesity related-cataract; however, the effect of coffee consumption on this type of cataract remains unknown. Four-week-old male C57BL/6JJms SLC mice were divided into two groups and were provided ad libitum access to either a control diet (control groups) or a HFD (HFD groups). The control groups and HFD groups were further divided into three or four subgroups for each experiment. Coffee intake markedly reduced the increase in body weight in a roasting-time and concentration-dependent manner. Coffee consumption also prevented the HFD-induced decrease in the concentration of GSH and AsA, and treatment with pyrocatechol or caffeine also restored the reduction of antioxidant compounds. Plasma cholesterol and triglycerides were significantly higher in HFD groups; however, coffee brew or coffee constituent treatment in the HFD-fed mice group prevented elevation of these levels. Caffeine is a major coffee component and pyrocatechol is generated thought the roasting process. These results revealed that caffeine and pyrocatechol in coffee brew may be the key constituents responsible for preventing the reduction of lens GSH and AsA in HFD-fed animals.

  • Epigenetic silencing of Lgr5 induces senescence of intestinal epithelial organoids during the process of aging

    Uchida R, Saito Y, Nogami K, Kajiyama Y, Suzuki Y, Kawase Y, Nakaoka T, Muramatsu T, Kimura K, Saito H

    Aging and Mechanisms of Disease (npj Aging and Mechanisms of Disease)  doi: 10.1038/s41514-018-0031-5 ( 1 )  2018.11

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2018, The Author(s). To understand the molecular features underlying stem cell aging, we established intestinal epithelial organoids derived from both young and aged mice and investigated alterations in their senescence and epigenetic status. Senescence-related changes including accumulation of senescence-associated β-galactosidase and up-regulation of Cdkn1a (p21) by DNA demethylation were observed in intestinal epithelial organoids derived from aged mice. We also demonstrated that the important stem cell marker Lgr5 was epigenetically silenced by trimethylation of histone H3 lysine 27, inducing suppression of Wnt signaling and a decrease of cell proliferation in organoids from aged mice. We further treated intestinal epithelial organoids from aged mice with nicotinamide mononucleotide (NMN), a key NAD+ intermediate. As a result, the organoids showed a higher NAD+ level, increased cell proliferative ability, activation of Lgr5 and suppression of senescence-associated genes, indicating that treatment with NMN could ameliorate senescence-related changes in intestinal epithelia. These findings suggest that organoids derived from aged animals could be a powerful research tool for investigating the molecular mechanisms underlying stem cell aging and for development of some form of anti-aging intervention, thus contributing to prolongation of healthy life expectancy.

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Reviews, Commentaries, etc. 【 Display / hide

Presentations 【 Display / hide

  • Pioglitazone ameliorates senescence related markers in visceral adipose tissue of obese mice.

    Masaki KIMURA, Risako ISHII,Natsumi HIRANO,Ryoei UCHIDA,Shoji YAMADA,Yoshimasa SAITO,Hidetsugu SAITO

    he 9th Federation of Asian and Oceanian Physiological Societies Congress (FAOPS), 2019.03, Poster (general)

  • マウス脂肪組織の老化関連マーカーに及ぼす高脂肪食摂取とピオグリタゾン投与の効果

    木村 真規、石井里沙子、平野菜摘、内田諒英、齋藤義正、Jonathan P.Whitehead,齋藤英胤

    第39回日本肥満学会大会 肥満研究24(Supplement): Pp.206 (神戸) , 2018.10, Poster (general)

  • Journal of Physical Fitness and Sports Medicine 7

    木村 真規、平野菜摘、石井里沙子、内田諒英、齋藤義正、齋藤英胤

    第73回日本体力医学会大会, 2018.09, Poster (general)

  • Effects of Environmental Enrichment on Physical Activity and Body Temperature in Mice.

    Masaki KIMURA, Risako ISHII, Natumi HIRANO, Ryoei UCHIDA, Yoshimasa SAITO, Hidetsugu SAITO

    23th Annual Congress of the European College of Sport Science,eBook of Abstracts (Dublin Irland) , 2018.07, Poster (general)

  • 高脂肪食摂取マウスの脂肪組織における老化関連マーカーの変化とピオグリタゾン投与の効果

    木村 真規、石井里沙子、平野菜摘、内田諒英、齋藤義正、齋藤英胤

    第18回日本抗加齢医学会総会 (大阪) , 2018.05, Poster (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Long term effects of diet and exercise in childhood on lipid metabolism and heat production related gene expression in liver and brown adipose tissue

    2019.04
    -
    2022.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 木村 真規, Grant-in-Aid for Scientific Research (C), Principal Investigator

  • 若齢期の食・運動習慣が肝臓・褐色脂肪の脂質代謝・熱産生関連遺伝子に及ぼす長期効果

    2019
    -
    2021

    科学研究費補助金(文部科学省・日本学術振興会), Research grant, Principal Investigator

  • PPARrアゴニストのアンチエイジング効果に関する研究

    2018
    -
    2019

    Keio Gijuku Academic Development Funds, Research grant, Principal Investigator

  • 若齢期の食・運動が成熟期の骨格筋・褐色・白色脂肪の代謝関連遺伝子に及ぼす長期効果

    2016
    -
    2018

    Grant-in-Aid for Scientific Research, Research grant, Principal Investigator

  • 高脂肪食摂取による腸管・肝臓・脂肪組織の老化関連遺伝子変化とDNAメチル化阻害薬の効果

    2016
    -
    2017

    慶應義塾学事振興資金, Research grant, Principal Investigator

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Awards 【 Display / hide

  • 日本抗加齢医学会 優秀演題賞

    内田諒英, 齋藤義正, 中岡哉彰, 村松俊英, 木村真規, 齋藤英胤., 2016.06, 日本抗加齢医学会, オルガノイド培養法により樹立した腸管上皮幹細胞におけるstem cell agingの検討.

    Type of Award: Awards of National Conference, Council and Symposium

     View Description

    第16回 日本抗加齢医学会総会, プログラム・抄録集:Pp.174/2016.06.10-12 (横浜)

  • 日本抗加齢医学会 優秀演題賞

    木村真規, 井手晴香, 白井栄里奈, 内田諒英, 齋藤義正, 齋藤英胤., 2016.06, 日本抗加齢医学会, 高脂肪食摂取時の脂肪組織における老化マーカー遺伝子の発現変化とDNAメチル化阻 害薬の効果.

    Type of Award: Awards of National Conference, Council and Symposium

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    第16回 総会 プログラム・抄録集: Pp.170/
    2016.06.10-12 (横浜)

  • 日本体力医学会若手奨励賞最優秀賞

    木村真規, 篠崎智一, 依田絵美, 山内秀樹, 鈴木政登, 柴崎敏昭., 2005.09, 日本体力医学会, 運動療法および食事療法時の体重減少速度と末梢性および中枢性摂食調節因子の関係—過食性肥満モデルOLETFラットを用いた検討—.

    Type of Award: Awards of National Conference, Council and Symposium

  • 日本体力医学会若手奨励賞

    篠崎智一, 依田絵美, 立石直人, 木村真規, 柴崎敏昭., 2004.09, 日本体力医学会, 運動療法・食事療法に伴う体重減少期の視床下部弓状核における摂食調節遺伝子発現の変化—過食性肥満モデルOLETFラットを用いた検討—.

    Type of Award: Awards of National Conference, Council and Symposium

  • 日本体力医学会 学会賞

    鈴木政登、穂積典子、清水桃子、高尾匡、町田勝彦、木村真規、中家優幸、真柄直郎、森豊, 2000, Japanese Journal of Physical Fitness and Sports Medicine, Effects of exercise and dietary therapies on renal function and morphological findings in the kidney and glucose-fatty metabolism in OLETF rats-Use of OLETF rats as a model of typeⅡdiabetes melitus-.

 

Courses Taught 【 Display / hide

  • THE CULTURE OF SPORTS, HEALTH, AND LONGEVITY IN JAPAN AND OTHER COUNTRIES

    2020

  • STUDY OF MAJOR FIELD: (PHARMACOTHERAPEUTICS)

    2020

  • SEMINAR:(PHARMACOTHERAPEUTICS)

    2020

  • RESEARCH FOR BACHELOR'S THESIS 1

    2020

  • PRE-CLINICAL TRAINING FOR HOSPITAL & COMMUNITY PHARMACY

    2020

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Memberships in Academic Societies 【 Display / hide

  • 日本消化器病学会, 

    2012.05
    -
    Present
  • 日本肝臓学会, 

    2012.03
    -
    Present
  • 日本抗加齢医学会, 

    2011.02
    -
    Present
  • 日本通風・核酸代謝学会, 

    2008.04
    -
    Present
  • 日本薬学会, 

    2005.08
    -
    Present

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Committee Experiences 【 Display / hide

  • 2012.05
    -
    Present

    会員, 日本肝臓学会

  • 2012.03
    -
    Present

    会員, 日本消化器病学会

  • 2011.02
    -
    Present

    会員, 日本抗加齢医学会

  • 2008.04
    -
    Present

    Member, 日本通風・核酸代謝学会

  • 2005.08
    -
    Present

    Member, 日本薬学会

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