Ichikawa, Daiju

写真a

Affiliation

Faculty of Pharmacy, Department of Pharmacy 病態生理学講座 (Shiba-Kyoritsu)

Position

Research Associate/Assistant Professor/Instructor
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Papers 【 Display / hide 】

  • GTN057, a komaroviquinone derivative, induced myeloma cells' death in vivo and inhibited c-MET tyrosine kinase

    Mikio Okayama, Kota Fujimori, Mariko Sato, Koichi Samata, Koki Kurita, Hiromu Sugiyama, Yutaka Suto, Genji Iwasaki, Taketo Yamada, Fumiyuki Kiuchi, Daiju Ichikawa, Maiko Matsushita, Maki Hirao, Hisako Kunieda, Kohei Yamazaki, Yutaka Hattori

    Cancer Med .  12 ( 8 ) 9749 - 9759 2023.04

    Research paper (scientific journal), Joint Work, Accepted

  • A thermoresponsive cationic block copolymer brush-grafted silica bead interface for temperature-modulated separation of adipose-derived stem cells

    Nagase K., Okada A., Matsuda J., Ichikawa D., Hattori Y., Kanazawa H.

    Colloids and Surfaces B: Biointerfaces (Colloids and Surfaces B: Biointerfaces)  220 2022.12

    ISSN  09277765

    　View Summary

    Adipose-derived mesenchymal stem cells (ADSCs) have beneficial effects in cell transplantation therapy; these cells are collected from adipose tissue using low-invasive methods. However, to prepare ADSCs for cell therapy, a cell separation method that neither involves modification of the cell surface nor causes loss of cell activity is needed. Here, we aimed to develop ADSC separation columns using thermoresponsive cationic block copolymer brush-grafted beads as packing materials. The block copolymer brush was formed by a bottom cationic segment, poly(N,N-dimethylaminopropylacrylamide) (PDMAPAAm), and an upper thermoresponsive segment, poly(N-isopropylacrylamide) (PNIPAAm), and was grafted in two atom transfer radical polymerization reactions. The copolymer brush-grafted silica beads were packed into a column. An ADSC suspension was introduced into the columns at 37 °C and adsorbed on the copolymer brush-modified beads through electrostatic and hydrophobic interactions with the PDMAPAAm and PNIPAAm segments, respectively. The adsorbed ADSCs eluted from the column by lowering the temperature to 4 °C. In contrast, most Jurkat and vascular endothelial cells eluted at 37 °C, because of the relatively weaker electrostatic interactions with the block copolymer brush compared to ADSCs. Using the prepared column, a mixture of ADSCs and Jurkat cells was separated by changing the column temperature. The recovered ADSCs exhibited cell activity. The developed cell separation column may be useful for isolating ADSCs without cell surface modification, while maintaining cell activity.

  • PAX5-positive plasma cell leukemia presenting as lymphocytosis

    Suzuka Tateno, Maki Hirao, Takahide Kikuchi, Yuiko Tsukada, Hisako Kunieda, Makoto Osada, Kohei Yamazaki, Ryunosuke Denda, Shigemichi Hirose, Maiko Matsushita, Daiju Ichikawa, Yutaka Hattori

    Rinsho Ketsueki 63 ( 10 ) 1415 - 1420 2022

    Research paper (scientific journal)

  • Thermally-modulated cell separation columns using a thermoresponsive block copolymer brush as a packing material for the purification of mesenchymal stem cells

    Kenichi Nagase , Goro Edatsune, Yuki Nagata, Junnosuke Matsudo, Daiju Ichikawa, Sota Yamada, Yutaka Hattori, Hideko Kanazawa

    Biomater Sci. 9 ( 21 ) 7054 - 7064 2021.10

    Research paper (scientific journal), Accepted

  • Antibody drug separation using thermoresponsive anionic polymer brush modified beads with optimised electrostatic and hydrophobic interactions

    Nagase K., Ishii S., Ikeda K., Yamada S., Ichikawa D., Akimoto A.M., Hattori Y., Kanazawa H.

    Scientific Reports (Scientific Reports)  10 ( 1 )  2020.12

    Research paper (scientific journal), Accepted

    　View Summary

    © 2020, The Author(s). Antibody drugs play an important role in biopharmaceuticals, because of the specificity for target biomolecules and reduction of side effects. Thus, separation and analysis techniques for these antibody drugs have increased in importance. In the present study, we develop functional chromatography matrices for antibody drug separation and analysis. Three types of polymers, poly(N-isopropylacrylamide (NIPAAm)-co-2-acrylamido-2-methylpropanesulfonic acid (AMPS)-co-N-phenyl acrylamide (PhAAm)), P(NIPAAm-co-AMPS-co-n-butyl methacrylate (BMA)), and P(NIPAAm-co-AMPS-co-tert-butylacrylamide (tBAAm)), were modified on silica beads through atom transfer radical polymerisation. Rituximab elution profiles were observed using the prepared beads-packed column. Rituximab adsorption at high temperature and elution at low temperature from the column were observed, as a result of the temperature-modulated electrostatic and hydrophobic interactions. Using the column, rituximab purification from contaminants was performed simply by changing the temperature. Additionally, three types of antibody drugs were separated using the column through temperature-modulated hydrophobic and electrostatic interactions. These results demonstrate that the temperature-responsive column can be applied for the separation and analysis of biopharmaceuticals through a simple control of the column temperature.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • Integrin β5 and β7 expression in lenalidomide-resistant multiple myeloma cells

    Hattori Y., Futo T., Uozaki R., Ichikawa D., Yamaguchi T., Yamamoto T., Matsushita M., Hirao M.

    International Journal of Hematology (International Journal of Hematology)  115 ( 4 ) 605 - 608 2022.04

    ISSN  09255710

  • E3 ubiquitin ligase in T cell anergy

    ICHIKAWA Daiju

    臨床免疫・アレルギー科 (科学評論社)  62 ( 4 ) 411 - 417 2014.10

    Article, review, commentary, editorial, etc. (scientific journal),  ISSN  1881-1930

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Presentations 【 Display / hide 】

  • Epigenetic factor CDYL2 attenuates lenalidomide snsitivity in multiple myeloma

    Daiju Ichikawa, Yuna Kamioka, Maiko Mastushita, Yutaka Hattori

    第48回日本骨髄腫学会学術集会 (東京, 日本) , 

    2023.05
    , 

    Poster presentation

  • Investigating the safety and efficacy of natural products that induce immunogenic cell death in multiple myeloma

    藤浪太晴, 栗田昴希, 松下麻衣子, 市川大樹, 須藤豊, 服部豊

    第48回日本骨髄腫学会学術集会 (東京, 日本) , 

    2023.05
    , 

    Oral presentation (general)

  • Epigenetic factor CDYL2 is associated with lenalidomide-resistance in multiple myeloma

    市川 大樹, 松下 麻衣子, 服部 豊

    日本薬学会 第143年会 (北海道札幌市) , 

    2023.03
    , 

    Poster presentation, 日本薬学会

  • 多発性骨髄腫に対して免疫原性細胞死を誘導するコマロビキノン誘導体の開発

    栗田昂希、松下麻衣子、田村快斗、市川大樹、須藤豊、岩崎源司、服部豊

    第84回日本血液学会学術集会, 

    2022.10
    , 

    Poster presentation

  • ドラッグリポジショニングにより見出されたアンブロキソールのオートファジー阻害を介した抗骨髄腫効果

    杉山裕夢、市川大樹、松下麻衣子、服部豊、他　

    第84回日本血液学会学術集会, 

    2022.10
    , 

    Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • エピゲノム制御による薬剤抵抗性難治性造血器腫瘍の克服

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(C), Principal investigator

  • Overcoming resistance to new drug in hematological malignancy tageting LncRNA.

    2019.04
    -
    2022.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

  • Identification of novel therapeutic target molecules and the molecular mechanism for refractory multiple myeloma

    2016.04
    -
    2018.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Principal investigator

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Awards 【 Display / hide 】

  • 優秀ポスター演題賞

    市川大樹, 2023.05, 第48回日本骨髄腫学会学術集会, Epigenetic factor CDYL2 attenuates lenalidomide sensitivity in multiple myeloma

    Type of Award： International academic award (Japan or overseas)

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Courses Taught 【 Display / hide 】

  • STUDY OF MAJOR FIELD: (CLINICAL PHYSIOLOGY AND THERAPEUTICS)

    2024

  • SPECIAL PRACTICE IN TISSUE CULTURE AND GENE TECHNOLOGY

    2024

  • SEMINAR: (CLINICAL PHYSIOLOGY AND THERAPEUTICS)

    2024

  • RESEARCH FOR BACHELOR'S THESIS 1

    2024

  • PRE-CLINICAL TRAINING FOR HOSPITAL & COMMUNITY PHARMACY

    2024

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