Papers - Arita, Makoto

Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast.

Hoshikawa Y, Shirota N, Tsugawa H, Kimura S, Matsuyama A, Yashiroda Y, Arita M, Fenical W, Yoshida M, *Nishimura S.

Proc Natl Acad Sci USA 122 ( 22 ) e2422126122 2025.05

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry.

*Schebb NH, Kampschulte N, Hagn G, Plitzko K, Meckelmann SW, Ghosh S, Joshi R, Kuligowski J, Vuckovic D, Botana MT, Sánchez-Illana Á, Zandkarimi F, Das A, Yang J, Schmidt L, Checa A, Roche HM, Armando AM, Edin ML, Lih FB, Aristizabal-Henao JJ, Miyamoto S, Giuffrida F, Moussaieff A, Domingues R, Rothe M, Hinz C, Das US, Rund KM, Taha AY, Hofstetter RK, Werner M, Werz O, Kahnt AS, Bertrand-Michel J, Le Faouder P, Gurke R, Thomas D, Torta F, Milic I, Dias IHK, Spickett CM, Biagini D, Lomonaco T, Idborg H, Liu JY, Fedorova M, Ford DA, Barden A, Mori TA, Kennedy PD, Maxey K, Ivanisevic J, Gallart-Ayala H, Gladine C, Wenk M, Galano JM, Durand T, Stark KD, Barbas C, Garscha U, Gelhaus SL, Ceglarek U, Flamand N, Griffin JL, Ahrends R, Arita M, Zeldin DC, Schopfer FJ, Quehenberger O, Julian R, Nicolaou A, Blair IA, Murphy MP, Hammock BD, Freeman B, Liebisch G, Serhan CN, Köfeler HC, Jakobsson PJ, Steinhilber D, Gelb MH, Holčapek M, Andrew R, Giera M, FitzGerald GA, Murphy RC, Newman JW, Dennis EA, Ekroos K, Milne GL, Gijón MA, Vesper HW, Wheelock CE, O'Donnell VB.

Science Signaling 18 ( 887 ) eadw1245 2025.05

Research paper (scientific journal), Joint Work, Accepted

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Porphyran from discolored nori prevents metabolic syndrome through microbiota-bile acid-ceramide pathway.

Yokoyama Y, Takashima Y, Morel JD, Taworntawat T, Kitamura N, Nagano U, Tanigaki T, Nakamura A, Miyazaki T, Taoka H, Mottis A, Perino A, Shinjo A, Suzuki N, Takekawa S, Fukuda S, Honda A, Ishihara K, Arita M, Tsubota K, Auwerx J, *Watanabe M.

iScience  28 ( 6 ) 112603 2025.05

Research paper (scientific journal), Joint Work, Accepted

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Transcriptomic and lipidomic analysis of aging-associated inflammatory signature in mouse liver.

*Ishihara T, Tsugawa H, Iwanami S, Chang JC, Minoda A, *Arita M.

Inflammation and Regeneration 45 ( 1 ) 13 2025.05

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Eosinophils and pleural macrophages counterregulate IL-33-elicited airway inflammation via the 12/15-lipoxygenase pathway.

Ito E, Hayashizaki R, Hosaka T, Yamane T, Miyata J, *Isobe Y, *Arita M.

Frontiers in Immunology 16   1565670 2025.04

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Data-independent acquisition coupled with electron-activated dissociation for in-depth structure elucidation of the fatty acid ester of hydroxy fatty acids.

Kurizaki Y, Matsuzawa Y, Takahashi M, Takeda H, Hasegawa M, Arita M, Miyamoto J, *Tsugawa H.

Analytical Chemistry 97 ( 14 ) 7861 - 7868 2025.04

Research paper (scientific journal), Joint Work, Accepted

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Celecoxib prevents malignant progression of smoking-induced lung tumors via suppression of the COX-2/PGE2 signaling pathway in mice.

Sakurai K, *Chubachi S, *Miyata J, Hamamoto J, Naganuma T, Shimada T, Otake S, Nakayama S, Irie H, Tsutsumi A, Kameyama N, Hegab AE, Shimoda M, Terai H, Yasuda H, Kanai Y, Arita M, Fukunaga K.

Front Immunol  16   1557790 2025.03

Research paper (scientific journal), Joint Work, Accepted

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12/15-lipoxygenase-derived electrophilic lipid modifications in phagocytic macrophages.

Deng K, *Isobe Y, Tsumagari K, Kato T, Arai H, Imami K, *Arita M.

ACS Chem. Biol. 20 ( 2 ) 357 - 368 2025.02

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Characterization of UGT8 as a monogalactosyl diacylglycerol synthase in mammals.

Ohba Y, Motohashi M, *Arita M.

J. Biochem. 177 ( 2 ) 141 - 152 2025.02

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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IL-5-producing group 2 innate lymphoid cells promote T cell-independent IgA production in cooperation with eosinophils.

*Yanagibashi T, Ikutani M, Nagai T, Arita M, Watanabe Y, Nagai Y, *Takatsu K.

Int. Immunol. in press 2024.12

Research paper (scientific journal), Joint Work, Accepted

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MS-DIAL 5 multimodal mass spectrometry data mining unveils lipidome complexities.

Takeda H, Matsuzawa Y, Takeuchi M, Takahashi M, Nizhida K, *Harayama T, Todoroki Y, Shimizu K, Sakamoto N, Oka T, Maekawa M, Chung MH, Kurizaki Y, Kiuchi S, Kvasnička A, Takeda U, Uchino H, Hasegawa M, Miyamoto J, Tanabe K, Takeda S, Mori T, Tanaka T, Yoshino T, Saito K, Okamoto M, Takahashi H, *Arita M, *Tsugawa H.

Nature Communications 15   9903 2024.11

Research paper (scientific journal), Joint Work, Corresponding author, Accepted

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MS2Lipid: A lipid subclass prediction program using machine learning and curated tandem mass spectral data.

Sakamoto N, Oka T, Matsuzawa Y, Nishida K, Jayaprakash J, Hori A, *Arita M, *Tsugawa H.

Metabolites 14   602 2024.11

Research paper (scientific journal), Corresponding author, Accepted

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Inflammatory profile of eosinophils in asthma-COPD overlap and eosinophilic COPD: A multi-omics study.

Sunata K, *Miyata J, Kawashima Y, Konno R, Ishikawa M, Hasegawa Y, Onozato R, Otsu Y, Matsuyama E, Sasaki H, Okuzumi S, Mochimaru T, Masaki K, Kabata H, Chubachi S, Arita M, Fukunaga K.

Frontiers in Immunology 15   1445769 2024.10

Research paper (scientific journal), Accepted

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Concordant inter-laboratory derived concentrations of ceramides in human plasma reference.

Torta F, Hoffmann N, Burla B, Alecu I, Arita M, Bamba T, Bennett SAL, Bertrand-Michel J, Brugger B, Cala MP, Camacho-Munoz D, Checa A, Chen M, Chocholouskova M, Cinel M, Chu-Van E, Colsch B, Coman C, Connell L, Sousa BD, Dickens AM, Fedorova M, Eriksson FF, Gallart-Ayala H, Ghorasaini M, Giera M, Guan XL, Haid M, Hankmeier T, Harms A, Horing M, Holcapek M, Hornemann T, Hu C, Hulsmeier A, Huynh K, Jones CM, Ivanisevic J, Izumi Y, Kofeler HC, Lam SM, Lange M, Lee JC, Liebisch G, Lippa K, Lopez-Clavijo AF, Manzi M, Martinefski MR, Math RGH, Mayor S, Meikle PJ, Monge ME, Moon MH, Muralidharan S, Nicolaou A, Nguyen-Tran T, O’Donnell VB, Oresic M, Ramanathan A, Riols F, Saigusa D, Schock TB, Schwartz-Zimmermann H, Shui G, Singh M, Takahashi M, Thorsteinsdottir M, Tomiyasu N, Tournadre A, Tsugawa H, Tyrrell V, van der Gugten G, Wakelam MO, Wheelock CE, Wolrab D, Xu G, Xu T, Boeden JA, *Ekroos K, *Ahrends R, *Wenk MR.

Nature Communications 15 ( 1 ) 8562 2024.10

Research paper (scientific journal), Joint Work, Accepted

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Commensal consortia decolonize Enterobacteriaceae via ecological control.

Furuichi M, Kawaguchi T, Pust M, Yasuma-Mitobe K, Plichta DR, Hasegawa N, Ohya T, Bhattarai S, Sasajima S, Aoto Y, Tuganbeav T, Yaginuma M, Ueda M, Okahashi N, Amafuji K, Kiridooshi Y, Sugita K, Strazer M, Avila-Pacheco J, Pierce K, Clish CB, Skelly AN, Hattori M, Nakamoto N, Caballero S, Norman JM, Olle B, Tanoue T, Suda W, Arita M, Bucci V, Atarashi K, *Xavier RJ, *Honda K.

Nature 633 ( 8031 ) 878 - 886 2024.09

Research paper (scientific journal), Joint Work, Accepted

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Group X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism via Host-Microbiota Interaction.

Sato H, Taketomi Y, Murase R, Park J, Hosomi K, Mizuguchi K, Arita M, Kunisawa J, *Murakami M.

Cell Reports 43 ( 10 ) 114752 2024.09

Research paper (scientific journal), Joint Work, Accepted

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MAFB in macrophages regulates prostaglandin E2-mediated lipid mediator class switch through ALOX15 in ischemic acute kidney injury.

Kanai M, Nishino T, Daassi D, Kimura A, Liao C, Shahri ZJ, Wakimoto A, Gogoleva N, Usui T, Morito N, Arita M, *Takahashi S, *Hamada M.

Journal of Immunology 213 ( 8 ) 1212 - 1224 2024.09

Research paper (scientific journal), Joint Work, Accepted

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Spatiotemporally distinct roles of cyclooxygenase-1 and cyclooxygenase-2 at fetomaternal interface in mice.

Aikawa S, Matsuo M, Akeda S, Sugimoto Y, Arita M, Isobe Y, Sugiura Y, Taira S, Maeda R, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Fukui Y, Hiraoka T, Harada M, Wada-Hiraike O, Osuga Y, *Hirota Y.

JCI Insight  9 ( 19 ) e181865 2024.08

Joint Work, Accepted

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Characterization of Entamoeba fatty acid elongases: validation as targets and provision of promising leads for new drugs against amebiasis.

*Mi-ichi F, Tsugawa H, Vo TK, Kurizaki Y, Yoshida H, Arita M.

PLoS Pathogens  20 ( 8 ) e1012435 2024.08

Research paper (scientific journal), Joint Work, Last author, Accepted

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Multi-omics analysis identified IL-4-induced IL1RL1high eosinophils characterized by prominent cysteinyl leukotriene metabolism.

Sunata K, *Miyata J, Kawashima Y, Konno R, Ishikawa M, Hasegawa Y, Onozato R, Otsu Y, Matsuyama E, Sasaki H, Okuzumi S, Mochimaru T, Masaki K, Kabata H, Kawana A, Arita M, Fukunaga K.

Journal of Allergy and Clinical Immunology  154 ( 5 ) 1277 - 1288 2024.07

Research paper (scientific journal), Accepted

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Fluorescence lifetime imaging of lipid heterogeneity in the inner mitochondrial membrane with a super-photostable environment-sensitive probe

Wang J, *Taki M, Ohba Y, Arita M, *Yamaguchi S

Angew Chem Int Ed Engl 63 ( 28 ) e202404328 2024.07

Research paper (scientific journal), Joint Work, Accepted

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Distinct roles of types 1 and 2 interferons in human eosinophil regulation: A multi-omics analysis

Sasaki H, *Miyata J, Kawashima Y, Konno R, Ishikawa M, Hasegawa Y, Onozato R, Otsu Y, Matsuyama E, Sunata K, Masaki K, Kabata H, Kimizuka Y, Ueki S, Asano K, Kawana A, Arita M, Fukunaga K.

Allergy 79 ( 11 ) 3141 - 3145 2024.07

Research paper (scientific journal), Joint Work, Accepted

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A lipidome landscape of aging in mice

*Tsugawa H, Ishihara T, Ogasa K, Iwanami S, Hori A, Takahashi M, Yamada Y, Satoh-Takayama N, Ohno H, Minoda A, *Arita M

Nature Aging 4 ( 5 ) 709 - 726 2024.04

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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TRPV4-dependent Ca2+ influx determines cholesterol dynamics at the plasma membrane.

Kuwashima Y, Yanagawa M, Maekawa M, Abe M, *Sako Y, *Arita M.

Biophysical journal 123   1 - 18 2024.03

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Glucosylceramide flippases contribute to cellular glucosylceramide homeostasis.

Kita N, Hamamoto A, Gowda SG, Takatsu H, Nakayama K, Arita M, Hui SP, *Shin HW

Journal of Lipid Research 65 ( 3 ) 100508 2024.01

Research paper (scientific journal), Joint Work, Accepted

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Lysosomal membrane integrity in fibroblasts derived from patients with Gaucher disease.

Hamamoto A, Kita N, Gowda SG, Takatsu H, Nakayama K, Arita M, Hui SP, *Shin HW.

Cell Struct Funct  49 ( 1 ) 1 - 10 2024.01

Research paper (scientific journal), Joint Work, Accepted

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Crucial role of single-stranded DNA binding in enhancing sensitivity to DNA-damaging agents for Schlafen 11 and Schlafen 13.

Fujiwara K, Maekawa M, Iimori Y, Ogawa A, Urano T, Kono N, Takeda H, Higashiyama H, Arita M, *Murai J.

iScience  26 ( 12 ) 108529 2023.12

Research paper (scientific journal), Joint Work, Accepted

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Application of liquid-liquid extraction for N-terminal myristoylation proteomics.

Tsumagari K, Isobe Y, Ishihama Y, Seita J, *Arita M, *Imami K.

Mol Cell Proteomics 22 ( 12 ) 100677 2023.11

Research paper (scientific journal), Joint Work, Corresponding author, Accepted

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Very-long-chain fatty acids (VLCFAs) are Crucial to Neuronal Polarity by Providing Sphingolipids to Lipid-Rafts.

Honda A, Nozumi M, Ito Y, Natsume R, Kawasaki A, Nakatsu F, Abe M, Uchino H, Matsushita N, Ikeda K, Arita M, Sakimura K, *Igarashi M.

Cell Reports 42 ( 10 ) 113195 2023.10

Research paper (scientific journal), Joint Work, Accepted

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Unique features of Entamoeba glycerophospholipid metabolism; has the Entamoeba lipid metabolism network evolved through gene loss and gain to enable parasitic life cycle adaptation?

*Mi-ichi F, Tsugawa H, Yoshida H, *Arita M.

mSphere  8 ( 5 ) e0017423 2023.10

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Long chain acyl-CoA synthetase 6 facilitates the local distribution of di-docosahexaenoic acid- and ultra-long-chain-PUFA-containing phospholipids in the retina to support normal visual function in mice

Kuroha S, Katada Y, Isobe Y, Uchino H, Shishikura K, Nirasawa T, Tsubota K, Negishi K, *Kurihara T, *Arita M.

FASEB Journal 37 ( 9 ) e23151 2023.09

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Gut microbiota carbohydrate metabolism contributes to insulin resistance.

Takeuchi T, *Kubota T, Nakanishi Y, Tsugawa H, Suda W, Kwon A, Yazaki J, Ikeda K, Nemoto S, Mochizuki Y, Kitami T, Yugi K, Mizuno Y, Yamamichi N, Yamazaki T, Takamoto I, Kubota N, Kadowaki T, Arner E, Carninci P, Ohara O, Arita M, Hattori M, Koyasu, S, *Ohno H.

Nature 621   389 - 395 2023.08

Research paper (scientific journal), Joint Work, Accepted

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Enhanced production of EPA-derived anti-inflammatory metabolites after oral administration of a novel self-emulsifying highly purified EPA ethyl ester formulation (MND-2119).

*Miyoshi T, Naoe S, Wakabayashi H, Yano T, Mori T, Kanda S, Arita M, Ito H.

J Atheroscler Thromb 30 ( 12 ) 1927 - 1949 2023.08

Research paper (scientific journal), Joint Work, Accepted

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Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34

Sugihara R, Taneike M, Murakawa T, Tamai T, Ueda H, Kitazume-Taneike R, Oka T, Akazawa Y, Nishida H, Mine K, Hioki A, Omi J, Omiya S, Aoki J, Ikeda K, Nishida K, Arita M, Yamaguchi O, *Otsu K

Nature Communications 14   4494 2023.07

Research paper (scientific journal), Joint Work, Accepted

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Gut microbiota-derived lipid metabolites facilitate regulatory T cell differentiation.

Shiratori H, Oguchi H, Isobe Y, Han KH, Sen A, Yakebe K, Takahashi D, Fukushima M, *Arita M, *Hase K

Scientific Reports 13   8903 2023.06

Research paper (scientific journal), Joint Work, Corresponding author, Accepted

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Essential lipid autacoids rewire mitochondrial energy efficiency in metabolic dysfunction-associated fatty liver disease.

*Lopez-Vicario C, Sebastian D, Casulleras M, Duran-Guell M, Flores-Costa R, Aguilar F, Lozano JJ, Zhang IW, Titos E, Kang JX, Zorzano A, Arita M, *Claria J.

Hepatology 77   1303 - 1318 2023.04

Research paper (scientific journal), Joint Work, Accepted

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Enzymatically-epoxidized docosahexaenoic acid, 19,20-EpDPE, suppresses hepatic crown-like structure formation and nonalcoholic steatohepatitis fibrosis through GPR120.

Aoki H, Isobe Y, Yoshida M, Kang JX, Maekawa M, *Arita M.

Biochim Biophys Acta Mol Cell Biol Lipids 1868 ( 3 ) 159275 2023.03

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Mitochondria directly sense osmotic stress to trigger rapid metabolic remodeling via regulation of pyruvate dehydrogenase (PDH) phosphorylation.

Ikizawa T, Ikeda K, Arita M, Kitajima S, Soga T, *Ichijo H, *Naguro I.

Journal of Biological Chemistry 299 ( 2 ) 102837 2023.02

Research paper (scientific journal), Joint Work, Accepted

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Dysregulated ceramide metabolism in mouse progressive dermatitis resulting from constitutive activation of Jak1

Iino Y, Naganuma T, *Arita M.

Journal of Lipid Research 64   100329 2023.02

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Computational mass spectrometry accelerates C=C position-resolved untargeted lipidomics using oxygen attachment dissociation

Uchino H, *Tsugawa H, Takahashi H, *Arita M.

Communications Chemistry 5   162 2022.12

Joint Work, Last author, Corresponding author, Accepted

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Sterol O-acyltransferase inhibition ameliorates high-fat diet-induced renal fibrosis and tertiary lymphoid tissue maturation after ischemic reperfusion injury.

Ariyasu Y, Sato Y, Isobe Y, Taniguchi K, *Yanagita M, *Arita M.

Int J Mol Sci  23 ( 24 ) 15465 2022.12

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Genetic deletion of Cyp4f18 disrupts the omega-3 epoxidation pathway and results in psoriasis-like dermatitis.

Yoshida M, Ishihara T, Isobe Y, *Arita M.

FASEB Journal 36 ( 12 ) e22648 2022.11

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Comprehensive analysis of 10-hydroxy-cis-12-octadecenoic acid (HYA) and related fatty acid metabolites produced by gut microbiota using LC-MS/MS-based lipidomics.

*Tsuji K, Shimada W, Kishino S, Ogawa J, Arita M.

Medical Mass Spectrometry 6 ( 2 ) 112 - 125 2022.11

Research paper (scientific journal), Joint Work, Last author, Accepted

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Pleiotropic roles of cholesteryl sulfate during Entamoeba encystation; involvement in cell rounding and development of membrane impermeability.

*Mi-ichi F, Tsugawa H, Arita M, Yoshida H.

mSphere  7   e0029922 2022.08

Research paper (scientific journal), Joint Work, Accepted

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Introducing the lipidomics minimal reporting checklist.

McDonald JG, Ejsing CS, Kopczynski D, Holcapek M, Aoki J, Arita M, Arita M, Baker ES, Bertrand-Michel J, Bowden JA, Brugger B, Ellis SR, Fedorova M, Griffiths WJ, Han X, Hartler J, Hoffmann N, Koelmel JP, Kofeler HC, Mitchell TW, O’Donnell VB, Saigusa D, Schwudke D, Shevchenko A, Ulmer CZ, Wenk MR, Witting M, Wolrab D, Xia Y, *Ahrends R, *Liebisch G, *Ekroos K.

Nature Metabolism 4   1086 - 1088 2022.08

Research paper (scientific journal), Joint Work, Accepted

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Microbiota-independent spontaneous dermatitis associated with increased sebaceous lipid production in Tmem79-deficient mice.

Morimoto K, Fukuda K, Ito Y, Tahara U, Sasaki T, Shiohama A, Kawasaki H, Kawakami E, Naganuma T, Arita M, Sasaki H, Koseki H, Matsui T, *Amagai M.

J Invest Dermatol  142 ( 11 ) 2864 - 2872 2022.06

Research paper (scientific journal), Joint Work, Accepted

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Lipidomic analysis revealed n-3 polyunsaturated fatty acids suppressed choroidal thinning and myopia progression in mice.

Mori K, Kuroha S, Hou J, Jeong H, Ogawa M, Ikeda S, Kang JX, Negishi K, Torii H, *Arita M, *Kurihara T, Tsubota K.

FASEB Journal 36   e22312 2022.06

Research paper (scientific journal), Joint Work, Corresponding author, Accepted

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Group IIA secreted phospholipase A2 controls skin carcinogenesis and psoriasis by shaping the gut microbiota.

Miki Y, Taketomi Y, Kidoguchi Y, Yamamoto K, Muramatsu K, Nishito Y, Park J, Hosomi K, Mizuguchi K, Kunisawa J, Soga T, Boilard E, Gowda SG, Ikeda K, Arita M, *Murakami M.

JCI Insight 7   e152611 2022.01

Research paper (scientific journal), Joint Work, Accepted

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The interaction of secreted phospholipase A2-IIA with the microbiota alters its lipidome and promotes inflammation.

Dore E, Joly-Beauparlant C, Morozumi S, Mathieu A, Levesque T, Allaeys I, Duchez A, Cloutier N, Leclercq M, Bodein A, Payre C, Martin C, Petit-Paitel A, Gelb M, Rangachari M, Murakami M, Davidovic L, Flamand N, Arita M, Lambeau G, Droit A, *Boilard E.

JCI Insight 7   e152638 2022.01

Research paper (scientific journal), Joint Work, Accepted

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Intestinal microbiome-dependent w3 lipid metabolite aKetoA prevents inflammatory diseases in mice and cynomolgus macaques.

Nagatake T, Kishino S, Urano E, Murakami H, Kitamura N, Konishi K, Ohno H, Tiwari P, Morimoto S, Node E, Adachi J, Abe Y, Isoyama J, Sawane K, Honda T, Inoue A, Uwamizu A, Matsuzaka T, Miyamoto Y, Hirata S, Saika A, Shibata Y, Hosomi K, Matsunaga A, Shimano H, Arita M, Aoki J, Oka M, Matsutani A, Tomonaga T, Kabashima K, Miyachi M, Yasutomi Y, Ogawa J, *Kunisawa J.

Mucosal Immunology 15   289 - 300 2022.01

Research paper (scientific journal), Joint Work, Accepted

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Oxidized phospholipids and neutrophil elastase coordinately play critical roles in NET formation.

Tokuhiro T, Ishizawa A, Sato H, Takita S, Yoshikawa A, Anzai R, Sato S, Aoyagi R, Arita M, Aratani Y, Shimizu S, Tanaka M, *Yotsumoto S.

Frontiers in Cell and Developmental Biology 9   718586 2021.09

Research paper (scientific journal), Joint Work, Accepted

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Comparative analysis of single-molecule dynamics of TRPV1 and TRPV4 channels in living cells

Kuwashima Y, *Yanagawa M, Abe M, Hiroshima M, Ueda M, Arita M, *Sako Y

Int J Mol Biol  22   8473 2021.08

Research paper (scientific journal), Joint Work, Accepted

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Long-chain saturated fatty acids in breast milk are associated with the pathogenesis of atopic dermatitis via induction of inflammatory ILC3s.

Kong WS, Tsuyama N, Inoue H, Guo Y, Mokuda S, Nobukiyo A, Nakatani N, Yamaide F, Nakano T, Kohno Y, Ikeda K, Nakanishi Y, Ohno H, Arita M, Shimojo N, *Kanno M.

Scientific Reports 11 ( 1 ) 13109 2021.06

Research paper (scientific journal), Accepted

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GPR120 signaling controls amyloid-β degrading activity of matrix metalloproteinases.

Kikuchi K, Tatebe T, Sudo Y, Yokoyama M, Kidana K, Chiu Y, Takatori S, Arita M, Hori Y, *Tomita T

Journal of Neuroscience 41 ( 28 ) 6173 - 6185 2021.06

Research paper (scientific journal), Joint Work, Accepted

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12/15-Lipoxygenase regulates IL-33-induced eosinophilic airway inflammation in mice.

Miyata J, Yokokura Y, Moro K, Arai H, Fukunaga K, *Arita M.

Frontiers in Immunology  12   687192 2021.05

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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ADIPOR1 deficiency-induced suppression of retinal ELOVL2 and docosahexaenoic acid levels during photoreceptor degeneration and visual loss.

Osada H, Toda E, Homma K, Guzman NA, Nagai N, Ogawa M, Negishi K, Arita M, Tsubota K, *Ozawa Y.

Cell Death and Disease 12 ( 5 ) 458 2021.05

Research paper (scientific journal), Joint Work, Accepted

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Staphylococcus cohnii is a potentially biotherapeutic skin commensal alleviating skin inflammation.

Ito Y, Sasaki T, Li Y, Tanoue T, Sugiura Y, Skelly AN, Suda W, Kawashima Y, Okahashi N, Watanabe E, Horikawa H, Shiohama A, Kurokawa R, Kawakami E, Iseki H, Kawasaki H, Iwakura Y, Shiota A, Yu L, Hisatsune J, Koseki H, Sugai M, Arita M, Ohara O, Matsui T, Suematsu M, Hattori M, Atarashi K, Amagai M, *Honda K.

Cell Reports 35 ( 4 ) 109052 2021.04

Research paper (scientific journal), Joint Work, Accepted

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Global profiling of gut microbiota-associated lipid metabolites in antibiotic-treated mice by LC-MS/MS-based analyses.

Okahashi N, Ueda M, Yasuda S, Tsugawa H, *Arita M.

STAR Protocols 2   100492 2021.04

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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ω3 fatty acid metabolite, 12-hydroxyeicosapentaenoic acid, alleviates contact hypersensitivity by downregulation of CXCL1 and CXCL2 gene expression in keratinocytes via retinoid X receptor α.

Saika A, Nagatake T, Hirata S, Sawane K, Adachi J, Abe Y, Isoyama J, Morimoto S, Node E, Tiwari P, Hosomi K, Matsunaga A, Honda T, Tomonaga T, Arita M, Kabashima K, *Kunisawa J.

FASEB Journal 35 ( 4 ) e21354 2021.04

Research paper (scientific journal), Joint Work, Accepted

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Analysis of lipid A in gram-negative intestinal bacteria using liquid chromatography-quadrupole time-of-flight mass spectrometry.

*Okahashi N, Ueda M, Matsuda F, *Arita M.

Metabolites 11   197 2021.03

Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

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Filopodium-derived vesicles produced by MIM enhance the migration of recipient cells.

Nishimura T, Oyama T, Hu HT, Fujioka T, Hanawa-Suetsugu K, Ikeda K, Yamada S, Kawana H, Saigusa D, Ikeda H, Kurata R, Oono-Yakura K, Kitamata M, Kida K, Hikita T, Mizutani K, Yasuhara K, Mimori-Kiyosue Y, Oneyama C, Kurimoto K, Hosokawa Y, Aoki J, Takai Y, Arita M, *Suetsugu S.

Developmental Cell 56 ( 6 ) 842 - 859 2021.03

Research paper (scientific journal), Joint Work, Accepted

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Stage-specific de novo synthesis of very long chain dihydroceramides confers dormancy in Entamoeba parasites.

*Mi-ichi F, Ikeda K, Tsugawa H, Deoler S, Yoshida H, *Arita M.

mSphere 6 ( 2 ) e00174-21 2021.03

Research paper (scientific journal), Joint Work, Accepted

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Frontline Science: Conversion of neutrophils into atypical Ly6G+SiglecF+ immune cells with neurosupportive potential in olfactory neuroepithelium.

Ogawa K, Asano K, Yotsumoto S, Yamane T, Arita M, Hayashi Y, Harada H, Makino-Okamura C, Fukuyama H, Kondo K, Yamasoba T, *Tanaka M.

Journal of Leukocyte Biology 109 ( 3 ) 481 - 496 2021.03

Research paper (scientific journal), Joint Work, Accepted

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Deletion of Alox15 improves kidney dysfunction and inhibits fibrosis by increased PGD2 in the kidney.

Takahashi N, Kikuchi H, Usui A, Furusho T, Fujimaru T, Fujiki T, Yanagi T, Matsuura Y, Asano K, Yamamoto K, Ando F, Susa K, Mandai S, Mori T, Rai T, Uchida S, *Arita M, *Sohara E.

Clinical and Experimental Nephrology 25 ( 5 ) 445 - 455 2021.02

Research paper (scientific journal), Joint Work, Accepted

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Functional lipidomics of vascular endothelial cells in response to laminar shear stress.

Hirata T, Yamamoto K, Ikeda K, *Arita M.

FASEB Journal 35   e21301 2021.02

Research paper (scientific journal), Joint Work, Accepted

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Senolysis by glutaminolysis inhibition ameliorates various age-associated disorders.

Johmura Y, Yamanaka T, Omori S, Wang T, Sugiura Y, Matsumoto M, Suzuki N, Kumamoto S, Yamaguchi K, Hatakeyama S, Takami T, Yamaguchi R, Shimizu E, Ikeda K, Okahashi N, Mikawa R, Suematsu M, Arita M, Sugimoto M, Nakayama K, Furukawa Y, Imoto S, *Nakanishi M.

Science 371   265 - 270 2021.01

Research paper (scientific journal), Joint Work, Accepted

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Helicobacter pylori metabolites exacerbate gastritis through C-type lectin receptors.

Nagata M, Toyonaga K, Ishikawa E, Haji S, Okahashi N, Takahashi M, Izumi Y, Imamura A, Takato K, Ishida H, Nagai S, Illarinov P, Stocker BL, Timmer M, Smith D, Williams SJ, Bamba T, Miyamoto T, Arita M, Appelmelk BJ, *Yamasaki S.

Journal of Experimental Medicine 218 ( 1 ) e20200815 2021.01

Research paper (scientific journal), Joint Work, Accepted

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Lipidomics links oxidized phosphatidylcholines and coronary arteritis in Kawasaki disease.

Nakashima Y, Sakai Y, Mizuno Y, Furuno K, Hirono K, Takatsuki S, Suzuki H, Onouchi Y, Kobayashi T, Tanabe K, Hamase K, Miyamoto T, Aoyagi R, Arita M, Yamamura K, Tanaka T, Nishio H, Takada H, Ohga S, Hara T.

Cardiovascular Research 117 ( 1 ) 96 - 108 2021.01

Research paper (scientific journal), Joint Work, Accepted

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Elucidation of gut microbiota-associated lipids using LC-MS/MS and 16S rRNA sequence analyses.

Yasuda S, Okahashi N, Tsugawa H, Ogata Y, Ikeda K, Suda W, Arai H, Hattori M, *Arita M.

iScience 23   101841 2020.12

Research paper (scientific journal), Joint Work, Accepted

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Transomics analysis reveals allosteric and gene regulation axes for altered hepatic glucose-responsive metabolism in obesity.

Kokaji T, Hatano A, Ito Y, Yugi K, Eto M, Morita K, Ohno S, Fujii M, Hironaka K, Egami R, Terakawa A, Tsuchiya T, Ozaki H, Inoue H, Uda S, Kubota H, Suzuki Y, Ikeda K, Arita M, Matsumoto M, Nakayama K, Hirayama A, Soga T, *Kuroda S.

Science Signaling 13 ( 660 ) eaaz1236 2020.12

Research paper (scientific journal), Joint Work, Accepted

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Maternal dietary imbalance between omega-6 and omega-3 fatty acids triggers the offspring’s overeating in mice.

*Sakayori N, Katakura M, Hamazaki K, Higuchi O, Fujii K, Fukabori R, Iguchi Y, Setogawa S, Takao K, Miyazawa T, Arita M, Kobayashi K.

Commun Biol  3   473 2020.08

Research paper (scientific journal), Joint Work, Accepted

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Eosinophils promote corneal wound healing via the 12/15-lipoxygenase pathway.

Ogawa M, Ishihara T, Isobe Y, Kato T, Kuba K, Imai Y, Uchino Y, Tsubota K, *Arita M.

FASEB Journal 34   12492 - 12501 2020.07

Research paper (scientific journal), Joint Work, Accepted

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A Lipidome Atlas in MS-DIAL 4

Tsugawa H, Ikeda K, Takahashi M, Satoh A, Mori Y, Uchino H, Okahashi N, Yamada Y, Tada I, Bonini P, Higashi Y, Okazaki Y, Zhou Z, Zhu ZJ, Koelmel J, Cajka T, Fiehn O, Saito K, Arita M, Arita M.

Nature Biotechnology in press 2020.06

Research paper (scientific journal), Joint Work, Accepted

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Antioxidant Vitamins and Lysophospholipids Are Critical for Inducing Mouse Spermatogenesis Under Organ Culture Conditions

Sanjo H, Yao T, Katagiri K, Sato T, Matsumura T, Komeya M, Yamanaka H, Yao M, Matsuhisa A, Asayama Y, Ikeda K, Kano K, Aoki J, Arita M, Ogawa T.

FASEB Journal 34   9480 - 9497 2020.05

Research paper (scientific journal), Joint Work, Accepted

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Maternal gut microbiota in pregnancy influences offspring metabolic phenotype.

Kimura I, Miyamoto J, Ohue-Kitano R, Watanabe K, Yamada T, Onuki M, Aoki R, Isobe Y, Kashihara D, Inoue D, Inaba A, Takamura Y, Taira S, Kumaki S, Watanabe M, Ito M, Nakagawa F, Irie J, Kakuta H, Shinohara M, Iwatsuki K, Tsujimoto G, Ohno H, Arita M, Itoh H, Hase K.

Science 367   eaaw8429 2020.02

Research paper (scientific journal), Joint Work, Accepted

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Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in mice.

Hirata SI, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, Ikeda K, Aoyagi R, Adachi J, Abe Y, Isoyama J, Suzuki H, Tomonaga T, Kiyono H, Kabashima K, Arita M, Kunisawa J.

Allergy 75   1935 - 1951 2020.02

Research paper (scientific journal), Joint Work, Accepted

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Homeistatic function of dermokine in the skin barrier and inflammation.

Utsunomiya A, Chino T, Utsunomiya N, Luong VH, Tokuriki A, Naganuma T, Arita M, Higashi K, Saito K, Suzuki N, Ohara A, Sugai M, Sugawara K, Tsuruta D, Oyama N, Hasegawa M.

J Invest Dermatol 140   838 - 849 2020

Research paper (scientific journal), Joint Work, Accepted

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Acyl-CoA synthetase 6 regulates long chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic processes in mice.

Shishikura K, Kuroha S, Matsueda S, Iseki H, Matsui T, Inoue A, Arita M.

FASEB J  33 ( 12 ) 14194 - 14203 2019.12

Research paper (scientific journal), Joint Work, Accepted

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Dietary omega-3 fatty acid ameliorates allergic rhinitis via eosinophilic production of the anti-allergic lipid mediator 15-hydroxyeicosapentaenoic acid in mice.

Sawane K, Nagatake T, Hosomi K, Hirata S, Adachi J, Abe Y, Isoyama J, Suzuki H, Matsunaga A, Fukumitsu S, Aida K, Tomonaga T, Arita M, Kunisawa J.

Nutrients 11   2868 2019.11

Research paper (scientific journal), Joint Work, Accepted

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Bi-directional crosstalk between neutrophils and adipocytes promotes adipose tissue inflammation.

Watanabe Y, Nagai Y, Honda H, Okamoto N, Yanagibashi T, Ogasawara M, Yamamoto S, Imamura R, Takasaki I, Hara H, Sasahara M, Arita M, Hida S, Taniguchi S, Suda T, Takatsu K.

FASEB J  33 ( 11 ) 11821 - 11835 2019.11

Research paper (scientific journal), Joint Work, Accepted

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Characterization of lipid profiles after dietary intake of polyunsaturated fatty acids using integrated untargeted and targeted lipidomics.

Naoe S, Tsugawa H, Takahashi M, Ikeda K, Arita M.

Metabolites 9   241 2019.10

Research paper (scientific journal), Joint Work, Accepted

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Gut microbiota confers host resistance to obesity by metabolizing dietary polyunsaturated fatty acids.

Miyamoto J, Igarashi M, Watanabe K, Karaki SI, Mukouyama H, Kishino S, Li X, Ichimura A, Irie J, Sugimoto Y, Mizutani T, Sugawara T, Miki T, Ogawa J, Drucker DJ, Arita M, Itoh H, Kimura I.

Nature Communications 10 ( 1 ) 4007 2019.09

Research paper (scientific journal), Joint Work, Accepted

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Preferential incorporation of administered eicosapentaenoic acid into thin-cap atherosclerotic plaques.

Sato T, Horikawa M, Takei S, Yamazaki F, Ito TK, Kondo T, Sakurai T, Kahyo T, Ikegami K, Sato S, Sato R, Jinno Y, Kawano H, Naoe S, Arita M, Kashiwagi Y, Setou M.

Arterioscler Thromb Vasc Biol 39 ( 9 ) 1802 - 1816 2019.09

Research paper (scientific journal), Joint Work, Accepted

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Dysregulated fatty acid metabolism in nasal polyp-derived eosinophils from patients with chronic rhinosinusitis

Miyata J., Fukunaga K., Kawashima Y., Watanabe T., Saitoh A., Hirosaki T., Araki Y., Kikawada T., Betsuyaku T., Ohara O., *Arita M.

Allergy: European Journal of Allergy and Clinical Immunology (Allergy: European Journal of Allergy and Clinical Immunology)  74 ( 6 ) 1113 - 1124 2019.06

Research paper (scientific journal), Joint Work, Accepted,  ISSN  01054538

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© 2019 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd. Background: Eosinophils are multifunctional granulocytes capable of releasing various cytokines, chemokines, and lipid mediators. We previously reported dysregulated fatty acid metabolism in peripheral blood-derived eosinophils from patients with severe asthma. However, functional characteristics of eosinophils present in allergic inflammatory tissues remain largely uncharacterized. Methods: We established a method for isolating CD69hi CCR3low CXCR4- siglec-8int eosinophils from nasal polyps of patients with eosinophilic rhinosinusitis (NP-EOS). Multi-omics analysis including lipidomics, proteomics, and transcriptomics was performed to analyze NP-EOS as compared to peripheral blood-derived eosinophils from healthy subjects (PB-EOS). Results: Lipidomic analysis revealed impaired synthesis of prostaglandins and 15-lipoxygenase (15-LOX)-derived mediators, and selective upregulation of leukotriene D4 production. Furthermore, proteomics and transcriptomics revealed changes in the expression of specific enzymes (GGT5, DPEP2, and 15-LOX) responsible for dysregulated lipid metabolism. Ingenuity pathway analysis indicated the importance of type 2 cytokines and pattern recognition receptor pathways. Stimulation of PB-EOS with eosinophil activators IL-5, GM-CSF, and agonists of TLR2 and NOD2 mimicked the observed changes in lipid metabolism. Conclusion: Inflammatory tissue-derived eosinophils possess a specific phenotype with dysregulated fatty acid metabolism that may be targeted therapeutically to control eosinophilic inflammatory diseases.

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Proteogenomic Analyses of Cellular Lysates Using a Phenol-Guanidinium Thiocyanate Reagent

Kawashima Y., Miyata J., Watanabe T., Shioya J., Arita M., Ohara O.

Journal of Proteome Research (Journal of Proteome Research)  18 ( 1 ) 301 - 308 2019.01

Research paper (scientific journal), Joint Work, Accepted,  ISSN  15353893

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© Copyright 2018 American Chemical Society. Protein and RNA profiles are highly informative when generating a comprehensive understanding of the dynamics of complex biological systems. Quantitative correlations between protein and RNA profiles are not always high, yet simultaneous acquisition of both profiles remains challenging, in part because of the limited availability of samples and the inconvenience of separately preparing protein and RNA fractions. In a previous study, protein, DNA, and RNA fractions were simultaneously prepared from the same sample using phenol-guanidinium isothiocyanate reagent (P/GTC), although the performance of P/GTC-extracts in proteogenomic analyses remains poorly understood. We therefore evaluated the performance of the P/GTC-extraction method in proteogenomic analyses using standard HEK293-F cells and human peripheral blood neutrophils. The latter cell type is renowned for its extreme vulnerability to protein/RNA degradation, reflecting high protease and RNase activities. Our data indicate that the P/GTC extraction method provides superior protein profiles from neutrophil and HEK293-F cell samples for simultaneous preparation of RNA and protein, as compared with those from conventional protein extraction methods. The P/GTC extraction method therefore provides a powerful and robust tool for a broad range of proteogenomic studies.

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Reciprocal regulation of STING and TCR signaling by mTORC1 for T-cell activation and function

Imanishi T., Unno M., Kobayashi W., Yoneda N., Matsuda S., Ikeda K., Hoshii T., Hirao A., Miyake K., Barber G., Arita M., Ishii K., Akira S., Saito T.

Life Science Alliance (Life Science Alliance)  2 ( 1 ) e201800282 2019

Research paper (scientific journal), Joint Work, Accepted

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© 2019 Imanishi et al. Stimulator of interferon genes (STING) plays a key role in detecting cytosolic DNA and induces type I interferon (IFN-I) responses for host defense against pathogens. Although T cells highly express STING, its physiological role remains unknown. Here, we show that costimulation of T cells with the STING ligand cGAMP and TCR leads to IFN-I production and strongly inhibits T-cell growth. TCR-mediated mTORC1 activation and sustained activation of IRF3 are required for cGAMP-induced IFN-I production, and the mTORC1 activity is partially counteracted by cGAMP, thereby blocking proliferation. This mTORC1 inhibition in response to costimulation depends on IRF3 and IRF7. Effector T cells produce much higher IFN-I levels than innate cells in response to cGAMP. Finally, we demonstrated that STING stimulation in T cells is effective in inducing antitumor responses in vivo. Our studies demonstrate that the outputs of STING and TCR signaling pathways are mutually regulated through mTORC1 to modulate T-cell functions.

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Mass spectrometry data repository enhances novel metabolite discoveries with advances in computational metabolomics

Tsugawa H., Satoh A., Uchino H., Cajka T., Arita M., Arita M.

Metabolites (Metabolites)  9 ( 6 ) E119 2019

Research paper (scientific journal), Joint Work, Accepted

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© 2019 Metabolites. All rights reserved. Mass spectrometry raw data repositories, including Metabolomics Workbench and MetaboLights, have contributed to increased transparency in metabolomics studies and the discovery of novel insights in biology by reanalysis with updated computational metabolomics tools. Herein, we reanalyzed the previously published lipidomics data from nine algal species, resulting in the annotation of 1437 lipids achieving a 40% increase in annotation compared to the previous results. Specifically, diacylglyceryl-carboxyhydroxy-methylcholine (DGCC) in Pavlova lutheri and Pleurochrysis carterae, glucuronosyldiacylglycerol (GlcADG) in Euglena gracilis, and P. carterae, phosphatidylmethanol (PMeOH) in E. gracilis, and several oxidized phospholipids (oxidized phosphatidylcholine, OxPC; phosphatidylethanolamine, OxPE; phosphatidylglycerol, OxPG; phosphatidylinositol, OxPI) in Chlorella variabilis were newly characterized with the enriched lipid spectral databases. Moreover, we integrated the data from untargeted and targeted analyses from data independent tandem mass spectrometry (DIA-MS/MS) acquisition, specifically the sequential window acquisition of all theoretical fragment-ion MS/MS (SWATH-MS/MS) spectra, to increase the lipidomic annotation coverage. After the creation of a global library of precursor and diagnostic ions of lipids by the MS-DIAL untargeted analysis, the co-eluted DIA-MS/MS spectra were resolved in MRMPROBS targeted analysis by tracing the specific product ions involved in acyl chain compositions. Our results indicated that the metabolite quantifications based on DIA-MS/MS chromatograms were somewhat inferior to the MS1-centric quantifications, while the annotation coverage outperformed those of the untargeted analysis of the data dependent and DIA-MS/MS data. Consequently, integrated analyses of untargeted and targeted approaches are necessary to extract the maximum amount of metabolome information, and our results showcase the value of data repositories for the discovery of novel insights in lipid biology.

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Resolvin D1 enhances the resolution of lung inflammation caused by long-term Pseudomonas aeruginosa infection.

Codagnone M, Cianci E, Lamolinara A, Cecilia Mari V, Nespoli A, Arita M, Bragonzi A, Iezzi M, Romano M, *Recchiuti A.

Mucosal Immunol (Mucosal Immunology)  11 ( 1 ) 35 - 49 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  19330219

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Pseudomonas aeruginosa lung infection is a main cause of disability and mortality worldwide. Acute inflammation and its timely resolution are crucial for ensuring bacterial clearance and limiting tissue damage. Here, we investigated protective actions of resolvin (Rv) D1 in lung infection induced by the RP73 clinical strain of P. aeruginosa. RvD1 significantly diminished bacterial growth and neutrophil infiltration during acute pneumonia caused by RP73. Inoculum of RP73, immobilized in agar beads, resulted in persistent lung infection up to 21 days, leading to a non resolving inflammation reminiscent of human pathology. RvD1 significantly reduced bacterial titer, leukocyte infiltration, and lung tissue damage. In murine lung macrophages sorted during P. aeruginosa chronic infection, RvD1 regulated the expression of Toll-like receptors, downstream genes, and microRNA (miR)-21 and 155, resulting in reduced inflammatory signaling. In vitro, RvD1 enhanced phagocytosis of P. aeruginosa by neutrophils and macrophages, recapitulating its in vivo actions. These results unveil protective functions and mechanisms of action of RvD1 in acute and chronic P. aeruginosa pneumonia, providing evidence for its potent pro-resolution and tissue protective properties on airway mucosal tissue during infection.

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12-OH-17,18-epoxyeicosatetraenoic acid alleviates eosinophilic airway inflammation in murine lungs.

Mochimaru T, *Fukunaga K, Miyata J, Matsusaka M, Masaki K, Kabata H, Ueda S, Suzuki Y, Goto T, Urabe D, Inoue M, Isobe Y, Arita M, Betsuyaku T.

Allergy (Allergy: European Journal of Allergy and Clinical Immunology)  73 ( 2 ) 369 - 378 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  01054538

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© 2017 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd. Background: Asthma is characterized by airway inflammation and obstruction with eosinophil infiltration into the airway. Arachidonic acid, an omega-6 fatty acid, is metabolized into cysteinyl leukotriene with pro-inflammatory properties for allergic inflammation, whereas the omega-3 fatty acid eicosapentaenoic acid (EPA) and its downstream metabolites are known to have anti-inflammatory effects. In this study, we investigated the mechanism underlying the counter-regulatory roles of EPA in inflamed lungs. Methods: Male C57BL6 mice were sensitized and challenged by ovalbumin (OVA). After EPA treatment, we evaluated the cell count of Bronchoalveolar lavage fluid (BALF), mRNA expressions in the lungs by q-PCR, and the amounts of lipid mediators by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based lipidomics. We investigated the effect of the metabolite of EPA by in vivo and in vitro studies. Results: Eicosapentaenoic acid treatment reduced the accumulation of eosinophils in the airway and decreased mRNA expression of selected inflammatory mediators in the lung. Lipidomics clarified the metabolomic profile in the lungs. Among EPA-derived metabolites, 12-hydroxy-17,18-epoxyeicosatetraenoic acid (12-OH-17,18-EpETE) was identified as one of the major biosynthesized molecules; the production of this molecule was amplified by EPA administration and allergic inflammation. Intravenous administration of 12-OH-17,18-EpETE attenuated airway eosinophilic inflammation through downregulation of C-C chemokine motif 11 (CCL11) mRNA expression in the lungs. In vitro, this molecule also inhibited the release of CCL11 from human airway epithelial cells stimulated with interleukin-4. Conclusion: These results demonstrated that EPA alleviated airway eosinophilic inflammation through its conversion into bioactive metabolites. Additionally, our results suggest that 12-OH-17,18-EpETE is a potential therapeutic target for the management of asthma.

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Stereoselective total synthesis of macrophage-produced prohealing 14,21-dihydroxydocosahexaenoic acids.

Nishimura K, Sakaguchi T, Nanba Y, Suganuma Y, Morita M, Hong S, Lu Y, Jun B, Bazan NG, Arita M, *Kobayashi Y.

J Org Chem (Journal of Organic Chemistry)  83 ( 1 ) 154 - 166 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00223263

　View Summary

© 2017 American Chemical Society. Synthesis of 14S,21R- and 14S,21S-dihydroxy-DHA (diHDHA) among the four possible stereoisomers of 14,21-diHDHA was studied. Methyl (R)-lactate (>97% ee), selected as a C20-C22 fragment (DHA numbering), was converted to the C17-C22 phosphonium salt, which was subjected to a Wittig reaction with racemic C16-aldehyde of the C12-C16 part with the TMS and TBS-oxy groups at C12 and C14, yielding the C12-C22 derivative with 14R/S and 21R chirality. Kinetic resolution using Sharpless asymmetric epoxidation of the TBS-deprotected allylic alcohol with l-(+)-DIPT/Ti(O-i-Pr) 4 afforded 14S-epoxy alcohol and 14R-allylic alcohol with >99% diastereomeric excess (de) for both. The CN group was introduced to the epoxy alcohol by reaction with Et 2 AlCN. The 14R-allylic alcohol was also converted to the nitrile via Mitsunobu inversion. Reduction of the nitrile with DIBAL afforded the key aldehyde corresponding to the C11-C22 moiety. The Wittig reaction of this aldehyde with a phosphonium salt of the remaining C1-C10 part followed by functional group manipulation gave 14S,21R-diHDHA. Similarly, ethyl (S)-lactate (>99% ee) was converted to 14S,21S-diHDHA. The chiral LC-UV-MS/MS analysis demonstrated that each of these two 14,21-diHDHAs synthesized using the presented total organic synthesis was highly stereoselective and identical to the macrophage-produced counterpart.

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Alveolar macrophages drive hepatocellular carcinoma lung metastasis by generating leukotriene B4.

Nosaka T, *Baba T, Tanabe Y, Sasaki S, Nishimura T, Imamura Y, Yurino H, Hashimoto S, Arita M, Nakamoto Y, Mukaida Y.

J Immunol (Journal of Immunology)  200 ( 5 ) 1839 - 1852 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00221767

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© 2018 by The American Association of Immunologists, Inc. All rights reserved. Macrophages in lungs can be classified into two subpopulations, alveolar macrophages (AMs) and interstitial macrophages (IMs), which reside in the alveolar and interstitial spaces, respectively. Accumulating evidence indicates the involvement of IMs in lung metastasis, but the roles of AMs in lung metastasis still remain elusive. An i.v. injection of a mouse hepatocellular carcinoma (HCC) cell line, BNL, caused lung metastasis foci with infiltration of AMs and IMs. Comprehensive determination of arachidonic acid metabolite levels revealed increases in leukotrienes and PGs in lungs in this metastasis model. A 5-lipoxygenase (LOX) inhibitor but not a cyclooxygenase inhibitor reduced the numbers of metastatic foci, particularly those of a larger size. A major 5-LOX metabolite, LTB 4 , augmented in vitro cell proliferation of human HCC cell lines as well as BNL cells. Moreover, in this lung metastasis course, AMs exhibited higher expression levels of the 5-LOX and LTB 4 than IMs. Consistently, 5-LOX-expressing AMs increased in the lungs of human HCC patients with lung metastasis, compared with those without lung metastasis. Furthermore, intratracheal clodronate liposome injection selectively depleted AMs but not IMs, together with reduced LTB 4 content and metastatic foci numbers in this lung metastasis process. Finally, IMs in mouse metastatic foci produced CCL2, thereby recruiting blood-borne, CCR2-expressing AMs into lungs. Thus, AMs can be recruited under the guidance of IM-derived CCL2 into metastatic lungs and can eventually contribute to the progression of lung metastasis by providing a potent arachidonic acid-derived tumor growth promoting mediator, LTB 4 .

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Reduction of lipid accumulation rescues Bietti’s crystalline dystrophy phenotypes.

Hata M, *Ikeda H, Iwai S, Iida Y, Gotoh N, Asaka I, Ikeda K, Isobe Y, Hori A, Nakagawa S, Yamato S, Arita M, Yoshimura N, Tsujikawa A.

Proc Natl Acad Sci USA (Proceedings of the National Academy of Sciences of the United States of America)  115 ( 15 ) 3936 - 3941 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00278424

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© 2018 National Academy of Sciences. All Rights Reserved. Bietti's crystalline dystrophy (BCD) is an intractable and progressive chorioretinal degenerative disease caused by mutations in the CYP4V2 gene, resulting in blindness in most patients. Although we and others have shown that retinal pigment epithelium (RPE) cells are primarily impaired in patients with BCD, the underlying mechanisms of RPE cell damage are still unclear because we lack access to appropriate disease models and to lesion-affected cells from patients with BCD. Here, we generated human RPE cells from induced pluripotent stem cells (iPSCs) derived from patients with BCD carrying a CYP4V2 mutation and successfully established an in vitro model of BCD, i.e., BCD patient-specific iPSC-RPE cells. In this model, RPE cells showed degenerative changes of vacuolated cytoplasm similar to those in postmortem specimens from patients with BCD. BCD iPSC-RPE cells exhibited lysosomal dysfunction and impairment of autophagy flux, followed by cell death. Lipidomic analyses revealed the accumulation of glucosylceramide and free cholesterol in BCD-affected cells. Notably, we found that reducing free cholesterol by cyclodextrins or δ-tocopherol in RPE cells rescued BCD phenotypes, whereas glucosylceramide reduction did not affect the BCD phenotype. Our data provide evidence that reducing intracellular free cholesterol may have therapeutic efficacy in patients with BCD.

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Identification of protein targets of 12/15-lipoxygenase-derived lipid electrophiles in mouse peritoneal macrophages using omega-alkynyl fatty acid.

Isobe Y, Kawashima Y, Ishihara T, Watanabe K, Ohara O, *Arita M.

ACS Chem Biol (ACS Chemical Biology)  13 ( 4 ) 887 - 893 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  15548929

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© 2018 American Chemical Society. The 12/15-lipoxygenase (12/15-LOX) enzyme introduces peroxyl groups, in a position-specific manner, into polyunsaturated fatty acids to form various kinds of bioactive lipid metabolites, including lipid-derived electrophiles (LDE). The resident peritoneal macrophage is the site of highest 12/15-LOX expression in the mouse. However, the role of the enzyme in the regulation of resident macrophages is not fully understood. Here, we describe a chemoproteomic method to identify the targets of enzymatically generated LDE. By treating mouse peritoneal macrophages with omega-alkynyl arachidonic acid (aAA), we identified a series of proteins adducted by LDE generated through a 12/15-LOX catalyzed reaction. Pathway analysis revealed a dramatic enrichment of proteins involved in energy metabolism and found that glycolytic flux and mitochondrial respiration were significantly affected by the expression of 12/15-LOX. Our findings thus highlight the utility of chemoproteomics using aAA for identifying intracellular targets of enzymatically generated LDE.

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Comprehensive analysis of the mouse cytochrome P450 family responsible for omega-3 epoxidation of eicosapentaenoic acid.

Isobe Y, Itagaki M, Ito Y, Naoe S, Kojima K, Ikeguchi M, *Arita M

Sci Rep (Scientific Reports)  8 ( 1 ) 7954 2018

Research paper (scientific journal), Joint Work, Accepted

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© 2018 The Author(s). Metabolites generated via oxygenation of the omega-3 double bond (omega-3 oxygenation) in eicosapentaenoic acid (EPA) have recently been identified as novel anti-inflammatory lipid mediators. Therefore, oxygenase(s) responsible for this metabolic pathway are of particular interest. We performed genome-wide screening of mouse cytochrome P450 (CYP) isoforms to explore enzymes involved in omega-3 oxygenation of EPA. As a result, 5 CYP isoforms (mouse Cyp1a2, 2c50, 4a12a, 4a12b, and 4f18) were selected and identified to confer omega-3 epoxidation of EPA to yield 17,18-epoxyeicosatetraenoic acid (17,18-EpETE). Stereoselective production of 17,18-EpETE by each CYP isoform was confirmed, and molecular modeling indicated that chiral differences stem from different EPA binding conformations in the catalytic domains of respective CYP enzymes.

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A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling.

Yamada M, Takahashi N, Matsuda Y, Sato K, Yokoji M, Sulijaya B, Maekawa T, Ushiki T, Mikami Y, Hayatsu M, Mizutani Y, Kishino S, Ogawa J, Arita M, Tabeta K, Maeda T, *Yamazaki K.

Sci Rep (Scientific Reports)  8 ( 1 ) 9008 2018

Research paper (scientific journal), Joint Work, Accepted

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© 2018 The Author(s). Several studies have demonstrated the remarkable properties of microbiota and their metabolites in the pathogenesis of several inflammatory diseases. 10-Hydroxy-cis-12-octadecenoic acid (HYA), a bioactive metabolite generated by probiotic microorganisms during the process of fatty acid metabolism, has been studied for its protective effects against epithelial barrier impairment in the intestines. Herein, we examined the effect of HYA on gingival epithelial barrier function and its possible application for the prevention and treatment of periodontal disease. We found that GPR40, a fatty acid receptor, was expressed on gingival epithelial cells; activation of GPR40 by HYA significantly inhibited barrier impairment induced by Porphyromonas gingivalis, a representative periodontopathic bacterium. The degradation of E-cadherin and beta-catenin, basic components of the epithelial barrier, was prevented in a GPR40-dependent manner in vitro. Oral inoculation of HYA in a mouse experimental periodontitis model suppressed the bacteria-induced degradation of E-cadherin and subsequent inflammatory cytokine production in the gingival tissue. Collectively, these results suggest that HYA exerts a protective function, through GPR40 signaling, against periodontopathic bacteria-induced gingival epithelial barrier impairment and contributes to the suppression of inflammatory responses in periodontal diseases.

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Facile determination of sphingolipids under alkali condition using metal-free column by LC-MS/MS.

Gowda SG, Ikeda K, *Arita M.

Anal Bioanal Chem (Analytical and Bioanalytical Chemistry)  410 ( 20 ) 4793 - 4803 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  16182642

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© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Extraction and analysis of sphingolipids from biological samples is a critical step in lipidomics, especially for minor species such as sphingoid bases and sphingosine-1-phosphate. Although several liquid chromatography-mass spectrometry methods enabling the determination of sphingolipid molecular species have been reported, they were limited in analytical sensitivity and reproducibility by causing significant peak tailing, especially by the presence of phosphate groups, and most of the extraction techniques are laborious and do not cover a broad range of sphingolipid metabolites. In this study, we developed a rapid single-phase extraction and highly sensitive analytical method for the detection and quantification of sphingolipids (including phosphates) comprehensively using liquid chromatography-triple quadruple mass spectrometry. After validating the reliability of the method, we analyzed the intestinal tissue sphingolipids of germ-free (GF) and specific pathogen-free (SPF) mice and found significantly higher levels of free sphingoid bases and sphingosine-1-phosphate in the GF condition as compared to the SPF condition. This method enables a rapid extraction and highly sensitive determination of sphingolipids comprehensively at low femtomolar ranges. [Figure not available: see fulltext.].

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17,18-EpETE-GPR40 axis ameliorates contact hypersensitivity by inhibiting neutrophil mobility in mice and cynomolgus macaques.

Nagatake T, Shiogama Y, Inoue A, Kikuta J, Honda T, Tiwari P, Kishi T, Yanagisawa A, Isobe Y, Matsumoto N, Shimojou M, Morimoto S, Suzuki H, Hirata S, Steneberg P, Edlund H, Aoki J, Arita M, Kiyono H, Yasutomi Y, Ishii M, Kabashima K, *Kunisawa J.

J Allergy Clin Immunol (Journal of Allergy and Clinical Immunology)  142 ( 2 ) 470 - 484 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00916749

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© 2017 The Authors Background: Metabolites of eicosapentaenoic acid exert various physiologic actions. 17,18-Epoxyeicosatetraenoic acid (17,18-EpETE) is a recently identified new class of antiallergic and anti-inflammatory lipid metabolite of eicosapentaenoic acid, but its effects on skin inflammation and the underlying mechanisms remain to be investigated. Objective: We evaluated the effectiveness of 17,18-EpETE for control of contact hypersensitivity in mice and cynomolgus macaques. We further sought to reveal underlying mechanisms by identifying the responsible receptor and cellular target of 17,18-EpETE. Methods: Contact hypersensitivity was induced by topical application of 2,4-dinitrofluorobenzene. Skin inflammation and immune cell populations were analyzed by using flow cytometric, immunohistologic, and quantitative RT-PCR analyses. Neutrophil mobility was examined by means of imaging analysis in vivo and neutrophil culture in vitro. The receptor for 17,18-EpETE was identified by using the TGF-α shedding assay, and the receptor's involvement in the anti-inflammatory effects of 17,18-EpETE was examined by using KO mice and specific inhibitor treatment. Results: We found that preventive or therapeutic treatment with 17,18-EpETE ameliorated contact hypersensitivity by inhibiting neutrophil mobility in mice and cynomolgus macaques. 17,18-EpETE was recognized by G protein–coupled receptor (GPR) 40 (also known as free fatty acid receptor 1) and inhibited chemoattractant-induced Rac activation and pseudopod formation in neutrophils. Indeed, the antiallergic inflammatory effect of 17,18-EpETE was abolished in the absence or inhibition of GPR40. Conclusion: 17,18-EpETE inhibits neutrophil mobility through GPR40 activation, which is a potential therapeutic target to control allergic inflammatory diseases.

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Thromboxane A2 facilitates IL-17A production from Vγ4+ γδT cells and promotes psoriatic dermatitis in mice.

1. Ueharaguchi Y, *Honda T, Kusuba N, Hanakawa S, Adachi A, Sawada Y, Otsuka A, Kitoh A, Dainichi T, Egawa G, Nakashima C, Nakajima S, Murata T, Ono S, Arita M, Narumiya S, Miyachi Y, Kabashima K.

J Allergy Clin Immunol (Journal of Allergy and Clinical Immunology)  142 ( 2 ) 680 - 683 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00916749

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MS-based lipidomics of human blood plasma – a community-initiated position paper to develop accepted guidelines.

Burla B, Arita M, Arita M, Bendt AK, Cazenave-Gassiot A, Dennis EA, Ekroos K, Han X, Ikeda K, Liebisch G, Lin MK, Loh TP, Meikle PJ, Oresic M, Quehenberger O, Shevchenko A, Torta F, Wakelam MJO, Wheelock CE, *Wenk MR

J Lipid Res (Journal of Lipid Research)  59 ( 10 ) 2001 - 2017 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  00222275

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Copyright © 2018 Burla et al. Published by The American Society for Biochemistry and Molecular Biology, Inc. Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.

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Reelin deficiency leads to aberrant lipid composition in mouse brain.

Mizukami T, Ikeda K, Shimanaka Y, Korogi K, Zhou C, Takase H, Tsuiji H, Kono N, Kohno T, Arai H, Arita M, *Hattori M.

Biochem Biophys Res Commun (Biochemical and Biophysical Research Communications)  505 ( 1 ) 81 - 86 2018

Research paper (scientific journal), Joint Work, Accepted,  ISSN  0006291X

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© 2018 Elsevier Inc. Reelin is a secreted protein essential for the development and function of the mammalian brain. The receptors for Reelin, apolipoprotein E receptor 2 and very low-density lipoprotein receptor, belong to the low-density lipoprotein receptor family, but it is not known whether Reelin is involved in the brain lipid metabolism. In the present study, we performed lipidomic analysis of the cerebral cortex of wild-type and Reelin-deficient (reeler) mice, and found that reeler mice exhibited several compositional changes in phospholipids. First, the ratio of phospholipids containing one saturated fatty acid (FA) and one docosahexaenoic acid (DHA) or arachidonic acid (ARA) decreased. Secondly, the ratio of phospholipids containing one monounsaturated FA (MUFA) and one DHA or ARA increased. Thirdly, the ratio of phospholipids containing 5,8,11-eicosatrienoic acid, or Mead acid (MA), increased. Finally, the expression of stearoyl-CoA desaturase-1 (SCD-1) increased. As the increase of MA is seen as an index of polyunsaturated FA (PUFA) deficiency, and the expression of SCD-1 is suppressed by PUFA, these results strongly suggest that the loss of Reelin leads to PUFA deficiency. Hence, MUFA and MA are synthesized in response to this deficiency, in part by inducing SCD-1 expression. This is the first report of changes of FA composition in the reeler mouse brain and provides a basis for further investigating the new role of Reelin in the development and function of the brain.

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An omega-3 polyunsaturated fatty acid derivative, 18-HEPE, protects against CXCR4-associated melanoma metastasis.

Li J, Chen CY, Arita M, Kim K, Li X, Zhang H, *Kang JX.

Carcinogenesis (Carcinogenesis)  39 ( 11 ) 1380 - 1388 2018

Research paper (scientific journal), Joint Work, Accepted

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Melanoma has a high propensity to metastasize and exhibits a poor response to classical therapies. Dysregulation of the chemokine receptor gene CXCR4 is associated with melanoma progression, and although n-3 polyunsaturated fatty acids (PUFAs) are known to be beneficial for melanoma prevention, the underlying mechanism of this effect is unclear. Here, we used the n-3 fatty acid desaturase (Fat-1) transgenic mouse model of endogenous n-3 PUFA synthesis to investigate the influence of elevated n-3 PUFA levels in a mouse model of metastatic melanoma. We found that relative to wild-type (WT) mice, Fat-1 mice exhibited fewer pulmonary metastatic colonies and improved inflammatory indices, including reduced serum tumor necrosis factor alpha (TNF-α) levels and pulmonary myeloperoxidase activity. Differential PUFA metabolites in serum were considered a key factor to alter cancer cell travelling to lung, and we found that n-6 PUFAs such as arachidonic acid induced CXCR4 protein expression although n-3 PUFAs such as eicosapentaenoic acid (EPA) decreased CXCR4 levels. In addition, serum levels of the bioactive EPA metabolite, 18-HEPE, were elevated in Fat-1 mice relative to WT mice, and 18-HEPE suppressed CXCR4 expression in B16-F0 cells. Moreover, relative to controls, numbers of pulmonary metastatic colonies were reduced in WT mice receiving intravenous injections either of 18-HEPE or 18-HEPE-pretreated melanoma cells. Our results indicate that 18-HEPE is a potential anticancer metabolite that mediates, at least in part, the preventive effect of n-3 PUFA on melanoma metastasis.

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PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis

Hirabayashi T, Anjo T, Kaneko A, Senoo Y, Shibata A, Takama H, Yokoyama K, Nishito Y, Ono T, Taya C, Muramatsu K, Fukami K, Muñoz-Garcia A, Brash AR, Ikeda K, Arita M, Akiyama M, *Murakami M.

Nature Commun 8   14609 2017

Research paper (scientific journal), Joint Work, Accepted

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Comprehensive identification of sphingolipid species by in silico retention time and tandem mass spectral library.

Tsugawa H, Ikeda K, Tanaka W, Senoo Y, *Arita M, *Arita M

J Cheminform 9   19 2017

Research paper (scientific journal), Joint Work, Accepted

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Prolonged activation of IL-5 producing ILC2 causes pulmonary arterial hypertrophy.

Ikutani M, Tsuneyama K, Kawaguchi K, Fukuoka J, Kudo F, Nakae S, Arita M, Nagai Y, Takaki S, *Takatsu K.

JCI Insight 2   e90721 2017

Research paper (scientific journal), Joint Work, Accepted

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Gut microbiota promotes obesity-associated liver cancer through PGE2-mediated suppression of antitumor immunity.

Loo TM, Kamachi F, Watanabe Y, Yoshimoto S, Kanda H, Arai Y, Nakajima-Takagi Y, Iwama A, Koga T, Sugimoto Y, Ozawa T, Nakamura M, Kumagai M, Watashi K, Taketo M, Aoki T, Narumiya S, Oshima M, Arita M, Hara E, *Ohtani N.

Cancer Discov 7   522 - 538 2017

Research paper (scientific journal), Joint Work, Accepted

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Autotaxin-lysophosphatidic acid-LPA3 signaling at the embryo-epithelial boundary controls decidualization pathways.

Aikawa S, Kano K, Inoue A, Wang J, Saigusa D, Nagamatsu T, Hirota Y, Fujii T, Tsuchiya S, Taketomi Y, Sugimoto Y, Murakami M, Arita M, Kurano M, Ikeda H, Yatomi Y, Chun J, *Aoki J.

EMBO J 36   2146 - 2160 2017

Research paper (scientific journal), Joint Work, Accepted

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Therapeutic effects of flurbiprofen axetil on mesenteric traction syndrome: randomized clinical trial.

Takahashi H, Shida D, Tagawa K, Iwamoto R, Arita M, Arai H, Suzuki T.

BMC Surg 17   90 2017

Research paper (scientific journal), Joint Work, Accepted

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Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library.

Aoyagi R, Ikeda K, Isobe Y, *Arita M

J Lipid Res 58   2229 - 2237 2017

Research paper (scientific journal), Joint Work, Accepted

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ω3 fatty acid epoxides are autocrine mediators that ensure full activation of mast cells.

Shimanaka Y, Kono N, Taketomi Y, Arita M, Okayama Y, Tanaka Y, Nishito Y, Mochizuki T, Kusuhara H, Adibekian A, Cravatt BF, *Murakami M, *Arai H.

Nat Med 23   1287 - 1297 2017

Research paper (scientific journal), Joint Work, Accepted

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Hyperoxidation of ether-linked phospholipids accelerates neutrophil extracellular trap formation.

Yotsumoto S, Muroi Y, Chiba T, Ohmura R, Yoneyama M, Magarisawa M, Dodo K, Terayama N, Sodeoka M, Aoyagi R, Arita M, Arakawa S, Shimizu S, *Tanaka M.

Sci Rep 7   16026 2017

Research paper (scientific journal), Joint Work, Accepted

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Maternal dietary balance between omega-6 and omega-3 polyunsaturated fatty acids impairs neocortical development via epoxy metabolites.

Sakayori N, Kikkawa T, Tokuda H, Kiryu E, Yoshizaki K, Kawashima H, Yamada T, Arai H, Kang JX, Katagiri H, Shibata H, Innis SM, Arita M, *Osumi N.

Stem Cells 34   470 - 482 2016

Research paper (scientific journal), Joint Work, Accepted

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Heterogenous fibroblasts underlie age-dependent tertially lymphoid tissues in the kidney.

Sato Y, Mii A, Hamazaki Y, Fujita H, Nakata H, Masuda K, Nishiyama S, Shibuya S, Haga H, Ogawa O, Shimizu A, Narumiya S, Kaisho T, Arita M, Yanagisawa M, Miyasaka M, Sharma K, Minato N, Kawamoto H, *Yanagita M.

JCI Insight １   e87680 2016

Research paper (scientific journal), Joint Work, Accepted

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Chronic arachidonic acid administration decreases docosahexaenoic acid and eicosapentaenoic acid-derived metabolites in kidneys of aged rats.

Katakura M, *Hashimoto M, Inoue T, Mamun AA, Tanabe Y, Arita M, Shido O.

PLoS One 10   e0140884 2015

Research paper (scientific journal), Joint Work, Accepted

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Resolvin E1 inhibits dendritic cell migration in the skin and attenuates contact hypersensitivity responses.

Sawada Y, *Honda T, Hanakawa S, Nakamizo S, Murata T, Ueharaguchi-Tanada Y, Ono S, Amano W, Nakajima S, Egawa G, Tanizaki H, Otsuka A, Kitoh A, Dainichi T, Ogawa N, Kobayashi Y, Yokomizo T, Arita M, Nakamura M, Miyachi Y, *Kabashima K.

J Exp Med 212   1921 - 1930 2015

Research paper (scientific journal), Joint Work, Accepted

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Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicostetraenoic acid in the gut.

*Kunisawa J, *Arita M, Hayasaka T, Harada T, Iwamoto R, Nagasawa R, Shikata S, Nagatake T, Suzuki H, Hashimoto E, Kurashima Y, Suzuki Y, Arai H, Setou M, Kiyono H.

Sci Rep 5   9750 2015

Research paper (scientific journal), Joint Work, Accepted

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Aspirin and eicosapentaenoic acid may arrest progressive IgA nephropathy: a potential alternative to immunosuppression.

*Hirahashi J, Hanafusa N, Wada T, Arita M, Hishikawa K, Hayashi M, Nangaku M

Intern Med 54   2377 - 2382 2015

Research paper (scientific journal), Joint Work, Accepted

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Neurite outgrowth stimulation by n3 and n6 polyunsaturated fatty acids of phospholipids in apolipoprotein E containing lipoproteins secreted from glial cells.

Nakato M, Matsuo M, Kono N, Arita M, Arai H, Ogawa J, Kioka N, *Ueda K.

J Lipid Res 56   1880 - 1890 2015

Research paper (scientific journal), Joint Work, Accepted

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Total synthesis of four stereoisomers of (4Z,7Z,10Z,12E,16Z,18E)-14,20-dihydroxy-4,7,10,12,16,18-docosahexaenoic acid and their anti-inflammatory activities.

Goto T, Urabe D, Masuda K, Isobe Y, Arita M, *Inoue M.

J Org Chem 80   7713 - 7726 2015

Research paper (scientific journal), Joint Work, Accepted

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Eosinophil-mediated signaling attenuates inflammatory responses in experimental colitis.

Masterson JC, McNamee EN, Fillon SA, Hosford L, Harris R, Fernando SD, Jedlicka P, Iwamoto R, Jacobsen E, Protheroe C, Eltzschig HK, Colgan SP, Arita M, Lee JJ, *Furuta GT.

Gut 64   1236 - 1247 2015

Research paper (scientific journal), Joint Work, Accepted

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A gut microbial metabolite of linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, ameliorates intestinal epithelial barrier impairment partially via GPR40/MEK-ERK pathway.

Miyamoto J, Mizukure T, Park S, Kishino S, Kimura I, Hirano K, Bergamo P, Rossi M, Suzuki T, Arita M, Ogawa J, *Tanabe S.

J Biol Chem 290   2902 - 2918 2015

Research paper (scientific journal), Joint Work, Accepted

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n-3 fatty acids effectively improve the reference memory-related learning ability associated with increased brain docosahexaenoic acid-derived docosanoids in aged rats.

*Hashimoto M, Katakura M, Tanabe Y, Al Mamun A, Inoue T, Hossain S, Arita M, Shido O.

Biochim Biophys Acta 1851   203 - 209 2015

Research paper (scientific journal), Joint Work, Accepted

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Combination therapy of 15-epi-lipoxin A4 with antibiotics protects mice from Escherichia coli-induced sepsis.

Ueda T, *Fukunaga K, Seki H, Miyata J, Arita M, Miyasho T, Obata T, Asano K, Betsuyaku T, Takeda J.

Crit Care Med 42   e288 - 295 2014

Research paper (scientific journal), Joint Work, Accepted

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The arachidonic acid metabolome as conserved regulator of cholesterol metabolism.

Demetz E, Schroll A, Auer K, Heim C, Patsch JR, Eller P, Theurl M, Lener D, Stanzl U, Seifert M, Haschka D, Asshoff M, Dichtl S, Nairz M, Huber E, Stadlinger M, Li X, Pallweber P, Scharnagl H, Stojakovic T, Marz W, Kleber ME, Garlaschelli K, Uboldi P, Catapano AL, Stellaard F, Rudling M, Kuba K, Imai Y, Arita M, Pramstaller PP, Tietge UJF, Trauner M, Norata GD, Claudel T, Hicks AA, Weiss G, *Tancevski I.

Cell Metab 20   787 - 798 2014

Research paper (scientific journal), Joint Work, Accepted

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Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis.

*Hirahashi J, Kawahata K, Arita M, Iwamoto R, Hishikawa K, Honda M, Hamasaki Y, Tanaka M, Okubo K, Kurosawa M, Takase O, Nakakuki M, Saiga K, Suzuki K, Kawachi S, Tojo A, Seki G, Marumo T, Hayashi M, Fujita T.

Sci Rep 4   6406 2014

Research paper (scientific journal), Joint Work, Accepted

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A design strategy for small molecule-based targeted MRI contrast agents: their application for detection of atherosclerotic plaques.

Iwaki S, Hokamura K, Ogawa M, Takehara Y, Muramatsu Y, Yamane T, Hirabayashi K, Morimoto Y, Hagisawa K, Nakahara K, Mineno T, Terai T, Komatsu T, Ueno T, Tamura K, Adachi Y, Hirata Y, Arita M, Arai H, Umemura K, Nagano T, *Hanaoka K.

Org Biomol Chem 12   8611 - 8618 2014

Research paper (scientific journal), Joint Work, Accepted

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18-HEPE, an n-3 fatty acid metabolite released by macrophages, prevents pressure overload-induced maladaptive cardiac remodeling.

Endo J, Sano M, Isobe Y, Fukuda K, Kang JX, Arai H, *Arita M.

J Exp Med 211   1673 - 1687 2014

Research paper (scientific journal), Joint Work, Accepted

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Identification of 14,20-dihydroxy-docosahexaenoic acid as a novel anti-inflammatory metabolite.

Yokokura Y, Isobe Y, Matsueda S, Iwamoto R, Goto T, Yoshioka T, Urabe D, Inoue M, Arai H, *Arita M.

J Biochem 156   315 - 321 2014

Research paper (scientific journal), Joint Work, Accepted

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Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice.

Tani Y, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H, *Arita M.

FASEB J 28   4036 - 4043 2014

Research paper (scientific journal), Joint Work, Accepted

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Rapidly rendering cells phagocytic through a cell-surface display technique and concurrent Rac activation.

Onuma H, *Komatsu T, Arita M, Hanaoka K, Ueno T, Terai T, Nagano T, *Inoue T.

Sci Signal 7   rs4 2014

Research paper (scientific journal), Joint Work, Accepted

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Regulation of intestinal IgA responses by dietary palmitic acid and its metabolism.

*Kunisawa J, Hashimoto E, Inoue A, Nagasawa R, Suzuki Y, Ishikawa I, Shikata S, Arita M, Aoki J, Kiyono H.

J Immunol 193   1666 - 1671 2014

Research paper (scientific journal), Joint Work, Accepted

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Eicosapentaenoic acid is converted via omega-3 epoxygenation to anti-inflammatory metabolite 12-hydroxy-17,18-epoxyeicosatetraenoic acid.

Kubota T, *Arita M, Isobe Y, Iwamoto R, Goto T, Yoshioka T, Urabe D, Inoue M, Arai H.

FASEB J 28   586 - 593 2014

Research paper (scientific journal), Joint Work, Accepted

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Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis.

Nakashima C, *Otsuka A, Kitoh A, Honda T, Egawa G, Nakajima S, Nakamizo S, Arita M, Kubo M, Miyachi Y, *Kabashima K.

J Allergy Clin Immunol 134   100 - 107 2014

Research paper (scientific journal), Joint Work, Accepted

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Matrix metalloproteinase (MMP)-9 in cancer-associated fibroblasts (CAFs) is suppressed by omega-3 polyusaturated fatty acids in vitro and in vivo.

Taguchi A, *Kawana K, Tomio K, Yamashita A, Isobe Y, Nagasaka K, Koga K, Inoue T, Nishida H, kojima S, Adachi K, Matsumoto Y, Arimoto T, Wada-Hiraike O, Oda K, kang JX, Arai H, *Arita M, Osuga Y, Fujii T.

PLoS One 27   e89605 2014

Research paper (scientific journal), Joint Work, Accepted

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Omega-3 fatty acids protect renal functions by increasing docosahexaenoic acid-derived metabolite levels in SHR.Cg-Leprcp/NDmcr rats, a metabolic syndrome model.

Katakura M, *Hashimoto M, Inoue T, Al Mamun A, Tanabe Y, Iwamoto R, Arita M, Tsuchikura S, Shido O.

Molecules 19   3247 - 3263 2014

Research paper (scientific journal), Joint Work, Accepted

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Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats.

Juman S, *Hashimoto M, Katakura M, Inoue T, Tanabe Y, Arita M, Miki T, Shido O.

Nutrients 5   1949 - 1961 2013

Research paper (scientific journal), Joint Work, Accepted

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Increased tissue level of omega-3 polyunsaturated fatty acids prevents pathological preterm birth.

Yamashita A, *Kawana K, Tomio K, Taguchi A, Isobe Y, Iwamoto R, Masuda K, Furuya H, Nagamatsu T, Nagasaka K, Oda K, Wada-Hiraike O, Yamashita T, Taketani Y, Kang JX, Arai H, *Arita M, Kozuma S, Fujii T.

Sci Rep 3   3113 2013

Research paper (scientific journal), Joint Work, Accepted

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Omega-3 polyunsaturated fatty acids suppress the cystic lesion formation of peritoneal endometriosis in transgenic mouse models.

Tomio K, *Kawana K, Taguchi A, Isobe Y, Iwamoto R, Yamashita A, Kojima S, Mori M, Nagamatsu T, Arimoto T, Oda K, Osuga Y, Taketani Y, Kang JX, Arai H, *Arita M, Kozuma S, Fujii T.

PLoS One 8   e73085 2013

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Polyunsaturated fatty acid saturation by gut lactic acid bacteria affecting host lipid composition.

Kishino S, Takeuchi M, Park S, Hirata A, Kitamura N, Kunisawa J, Kiyono H, Iwamoto R, Isobe Y, Arita M, Arai H, Ueda K, Shima J, Takahashi S, Yokozeki K, Shimizu S, *Ogawa J.

Proc Natl Acad Sci USA 110   17808 - 17813 2013

Research paper (scientific journal), Joint Work, Accepted

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The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza.

Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanabe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, *Imai Y.

Cell 153   112 - 125 2013

Research paper (scientific journal), Joint Work, Accepted

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Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma.

Miyata J, Fukunaga K, Iwamoto R, Isobe Y, Niimi K, Takamiya R, Takihara T, Tomomatsu K, Suzuki Y, Oguma T, Sayama K, Arai H, Betsuyaku T, Arita M, *Asano K.

J Allergy Clin Immunol 131   353 - 360 2013

Research paper (scientific journal), Joint Work, Accepted

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Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.

Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, Tanaka S, Sakanaka M, Nakamura M, Nishito Y, Kawana M, Kambe N, Ikeda K, Taguchi R, Nakamizo S, Kabashima K, Gelb MH, Arita M, Yokomizo T, Nakamura M, Watanabe K, Hirai H, Nakamura M, Okayama Y, Ra C, Aritake K, Urade Y, Morimoto K, Sugimoto Y, Shimizu T, Narumiya S, Hara S, *Murakami M.

Nat Immunol 14   554 - 563 2013

Research paper (scientific journal), Joint Work, Accepted

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Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators.

Miki Y, Yamamoto K, Taketomi Y, Sato H, Shimo K, Kobayashi T, Ishikawa Y, Ishii T, Nakanishi H, Ikeda K, Taguchi R, Kabashima K, Arita M, Arai H, Lambeau G, Bollinger JM, Hara S, Gelb MH, *Murakami M.

J Exp Med 210   1217 - 1234 2013

Research paper (scientific journal), Joint Work, Accepted

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Stereochemical assignment and anti-inflammatory properties of the omega-3 lipid mediator resolvin E3.

Isobe Y, *Arita M, Iwamoto R, Urabe D, Todoroki H, Masuda K, Inoue M, Arai H.

J Biochem 153   355 - 360 2013

Research paper (scientific journal), Joint Work, Accepted

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Inflammation and resolution are associated with upregulation of fatty acid beta-oxidation in zymosan-induced peritonitis.

Fujieda Y, Manno A, Hayashi Y, Rhodes N, Guo L, Arita M, Bamba T, *Fukusaki E.

PLoS One 8   e66270 2013

Research paper (scientific journal), Joint Work, Accepted

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Global metabolomic analysis of heart tissue in a hamster model for dilated cardiomyopathy.

*Maekawa K, Hirayama A, Iwata Y, Tajima Y, Nishimaki-Mogami T, Sugawara S, Ueno N, Abe H, Ishikawa M, Murayama M, Matsuzawa Y, Nakanishi H, Ikeda K, Arita M, Taguchi R, Minamino N, Wakabayashi S, Soga T, Saito Y.

J Mol Cell Cardiol 59   76 - 85 2013

Research paper (scientific journal), Joint Work, Accepted

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Lipidomic analysis of brain tissues and plasma in a mouse model expressing mutated human amyloid precursor protein/tau for Alzheimer's disease.

Tajima Y, Ishikawa M, Maekawa K, Murayama M, Senoo Y, Nishimaki-Mogami T, Nakanishi H, Ikeda K, Arita M, Taguchi R, Okuno A, Mikawa R, Niida S, Takikawa O, *Saito Y.

Lipids Health Dis 12   68 2013

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Identification and structure determination of novel anti-inflammatory mediator resolvin E3: 17,18-dihydroxyeicosapentaenoic acid.

Isobe Y, *Arita M, Matsueda S, Iwamoto R, Fujihara T, Nakanishi H, Taguchi R, Masuda K, Sasaki K, Urabe D, Inoue M, Arai H.

J Biol Chem 287   10525 - 10534 2012

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1 maintains macrophage function under cigarette smoke-induced oxidative stress.

*Takamiya R, Fukunaga K, Arita M, Miyata J, Seki H, Minematsu N, Suematsu M, Asano K.

FEBS Open Bio 2   328 - 333 2012

Research paper (scientific journal), Joint Work, Accepted

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LPIAT1 regulates arachidonic acid content in phosphatidylinositol and is required for cortical lamination in mice.

Lee HC, Inoue T, Sasaki J, Kubo T, Matsuda S, Nakasaki Y, Hattori M, Tanaka F, Udagawa O, Kono N, Itoh T, Ogiso H, Taguchi R, Arita M, Sasaki T, *Arai H.

Mol Biol Cell 23   4689 - 4700 2012

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Total synthesis and bioactivities of two proposed structures of maresin.

Sasaki K, Urabe D, Arai H, Arita M, *Inoue M.

Chem Asian J 6   534 - 543 2011

Research paper (scientific journal), Joint Work, Accepted

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Eosinophils promote resolution of acute peritonitis by producing pro-resolving mediators in mice.

Yamada T, Tani Y, Nakanishi H, Taguchi R, *Arita M, Arai H.

FASEB J 25   561 - 568 2011

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Development and application of a near-infrared fluorescent probe for oxidative stress based on differential reactivity of linked cyanine dyes.

Oushiki D, Kojima H, Terai T, Arita M, Hanaoka K, Urano Y, *Nagano T.

J Am Chem Soc 132   2795 - 2801 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Transgenic restoration of long chain omega-3 fatty acids in insulin target tissues improves resolution capacity and alleviates obesity-linked inflammation and insulin resistance in high fat-fed mice.

White PJ, Arita M, Taguchi R, Kang JX, *Marette A.

Diabetes 59   3066 - 3073 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

The anti-inflammatory and pro-resolving mediator resolvin E1 protects mice from bacterial pneumonia and acute lung injury.

Seki H, Fukunaga K, Arita M, Arai H, Nakanishi H, Taguchi R, Miyasho T, Takamiya R, Asano K, Ishizaka A, Takeda J, *Levy BD.

J Immunol 184   836 - 843 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1 receptor activation signals phosphorylation and phagocytosis.

Ohira T, Arita M, Omori K, Recchiuti A, Van Dyke TE, *Serhan CN.

J Biol Chem 285   3451 - 3461 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Neuroprotectin D1/protectin D1 stereoselective and specific binding with human retinal pigment epithelial cells and neutrophils.

Marcheselli VL, Mukherjee PK, Arita M, Hong S, Antony R, Sheets K, Winkler JW, Petasis NA, Serhan CN, *Bazan NG.

Prostaglandins Leukot Essent Fatty Acids 82   27 - 34 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Lipoxin A4 reduces lipopolysaccharide-induced inflammation in macrophages and intestinal epithelial cells through inhibition of NF-kB activation.

Kure I, Nishiumi S, Nishitani Y, Tanoue T, Ishida T, Mizuno M, Fujita T, Kusumi H, Arita M, Azuma T, *Yoshida M.

J Pharmacol Exp Ther 332   541 - 548 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1, an endogenous lipid mediator derived from eicosapentaenoic acid, prevents dextran sulfate sodium-induced colitis.

Ishida T, *Yoshida M, Arita M, Nishitani Y, Nishiumi S, Masuda A, Mizuno S, Takagawa T, Morita Y, Kusumi H, Inokuchi H, Serhan CN, Blumberg RS, Azuma T.

Inflamm Bowel Dis 16   87 - 95 2010

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Anti-angiogenesis effect of the novel anti-inflammatory and pro-resolving lipid mediators.

Jin Y, Arita M, Zhang Q, Saban DR, Chauhan SK, Chiang N, Serhan CN, *Dana R.

Invest Opthalmol Vis Sci 50   4743 - 4752 2009

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Total synthesis and bioactivity of resolvin E2.

Ogawa S, Urabe D, Yokokura Y, Arai H, Arita M, *Inoue M.

Org Lett 11   3602 - 3605 2009

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1, and EPA-derived mediator in whole blood, selectively counterregulates leukocytes and platelets.

Dona M, Fredman G, Schwab JM, Chiang N, Arita M, Goodarzi A, Cheng G, von Andrian UH, *Serhan CN.

Blood 112   848 - 855 2008

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1 Selectively Interacts with Leukotriene B4 Receptor BLT1 and ChemR23 to Regulate Inflammation.

Arita M, Ohira T, Sun YP, Elangovan S, Chiang N, *Serhan CN.

J Immunol 178   3912 - 3917 2007

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1 promotes mucosal surface clearance of neutrophils: a new paradigm for inflammatory resolution.

Campbell EL, Louis NA, Tomassetti SE, Canny GO, Arita M, Serhan CN, *Colgan SP.

FASEB J 21   3162 - 3170 2007

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1 and protectin D1 activate inflammation-resolution programmes.

Schwab JM, Chiang N, Arita M, *Serhan CN.

Nature 447   869 - 874 2007

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Regulation of hepatic cholesterol synthesis by a novel protein (SPF) that accelerates cholesterol biosynthesis.

Shibata N, Jishage K, Arita M, Watanabe M, Kawase Y, Nishikawa K, Natori Y, Inoue H, Shimano H, Yamada N, Tsujimoto M, *Arai H.

FASEB J 20   2642 - 2644 2006

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Metabolic inactivation of resolvin E1 and stabilization of its anti- inflammatory actions.

Arita M, Oh SF, Chonan T, Hong S, Elangovan S, Sun YP, Uddin J, Petasis NA, *Serhan CN.

J Biol Chem 281   22847 - 22854 2006

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Leukotriene B4 and lipoxin A4 are counterregulatory signals for neural stem cell proliferation and differentiation.

Wada K, Arita M, Nakajima A, Katayama K, Kudo C, Kamisaki Y, *Serhan CN.

FASEB J 20   1785 - 1792 2006

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Identification and functional characterization of a presqualene diphosphate phosphatase.

Fukunaga K, Arita M, Takahashi M, Morris AJ, Pfeffer M, *Levy BD.

J Biol Chem 281   9490 - 9497 2006

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis.

Tjonahen E, Oh SF, Siegelman J, Elangovan S, Percarpio K, Hong S, Arita M, *Serhan CN.

Chem Biol 13   1193 - 1202 2006

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

RvE1 protects from local inflammation and osteoclast mediated bone destruction in periodontitis.

Hasturk H, Kantarci IA, Ohira T, Arita M, Ebrahimi N, Petasis N, Levy BD, Serhan CN, *Van Dyke TE.

FASEB J 20   401 - 403 2006

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Molecular circuits of resolution: formation and actions of resolvins and protectins.

Bannenberg GL, Chiang N, Ariel A, Arita M, Tjonahen E, Gotlinger K, Hong S, *Serhan CN.

J Immunol 174   4345 - 4355 2005

Research paper (scientific journal), Joint Work, Accepted

Stereochemical assignment, anti-inflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1.

Arita M, Bianchini F, Aliberti J, Sher A, Chiang N, Hong S, Yang R, Petasis NA, *Serhan CN.

J Exp Med 201   713 - 722 2005

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Resolvin E1, an endogenous lipid mediator derived from omega-3 eicosapentaenoic acid, protects against 2,4,6-trinitrobenzene sulfonic acid-induced colitis.

Arita M, Yoshida M, Hong S, Tjonahen E, Glickman J, Petasis NA, Blumberg RS, *Serhan CN.

Proc Natl Acad Sci USA 102   7671 - 7676 2005

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

A stable aspirin-triggered lipoxin A4 analog blocks phosphorylation of leukocyte-specific protein 1 in human neutrophils.

Ohira T, Bannenberg G, Arita M, Takahashi M, Ge Q, Van Dyke TE, Stahl GL, Serhan CN, *Badway JA.

J Immunol 173   2091 - 2098 2004

Research paper (scientific journal), Joint Work, Accepted

Human ALX receptor regulates neutrophil recruitment in transgenic mice: roles in inflammation and host defense.

Devchand PR, Arita M, Hong S, Bannenberg GL, Moussignac RL, Gronert K, *Serhan CN.

FASEB J 17   652 - 659 2003

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Aspirin-triggered lipoxin A4 and B4 analogs block extracellular signal-regulated kinase-dependent TNF-alpha secretion from human T cells.

Ariel A, Chiang N, Arita M, Petasis NA, *Serhan CN.

J Immunol 170   6266 - 6272 2003

Research paper (scientific journal), Joint Work, Accepted

Lipoxin A4 and aspirin-triggered 15-epi-lipoxin A4 inhibit human neutrophil migration: comparisons between synthetic 15 epimers in chemotaxis and transmigration with microvessel endothelial cells and epithelial cells.

Fierro IM, Colgan SP, Bernasconi G, Petasis NA, Clish CB, Arita M, *Serhan CN.

J Immunol 170   2688 - 2694 2003

Research paper (scientific journal), Joint Work, Accepted

PH-dependent translocation of alpha-tocopherol transfer protein (alpha-TTP) between hepatic cytosol and late endosomes.

Horiguchi M, Arita M, Kaempf-Rotzoll DE, Tsujimoto M, Inoue K, *Arai H.

Genes Cells 8   789 - 800 2003

Research paper (scientific journal), Joint Work, Accepted

Delayed-onset ataxia in mice lacking alpha-tocopherol transfer protein: Model for neuronal degeneration caused by chronic oxidative stress.

*Yokota T, Igarashi K, Uchihara T, Jishage K, Tomita H, Inaba A, Li Y, Arita M, Suzuki H, Mizusawa H, Arai H.

Proc Natl Acad Sci USA 98   15185 - 15190 2001

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis.

Shibata N, Arita M, Misaki Y, Dohmae N, Takio K, Ono T, Inoue K, *Arai H.

Proc Natl Acad Sci USA 98   2244 - 2249 2001

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Alpha-tocopherol transfer protein is important for the normal development of placental labyrinthine trophoblasts in mice.

Jishage K, Arita M, Igarashi K, Iwata T, Watanabe M, Ogawa M, Ueda O, Kamada N, Inoue K, Arai H, *Suzuki H.

J Biol Chem 276   1669 - 1672 2001

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Postmortem study of ataxia with retinitis pigmentosa by mutation of the alpha-tocopherol transfer protein gene.

*Yokota T, Uchihara T, Kumagai I, Shiojiri T, Pang J, Arita M, Arai H, Hayashi M, Kiyosawa M, Okeda R, Mizusawa H.

J Neurol Neurosurg Psychiatry 68   521 - 525 2000

Research paper (scientific journal), Joint Work, Accepted

Different functional aspects of the group II subfamily (Type IIA and V) and type X secretory phospholipase A(2)s in regulating arachidonic acid release and prostagrandin generation.

Murakami M, Kambe T, Shimbara S, Higashino K, Hanasaki K, Arita H, Horiguchi M, Arita M, Arai H, Inoue K, *Kudo I.

J Biol Chem 274   31435 - 31444 1999

Research paper (scientific journal), Joint Work, Accepted

Effect of alpha-tocopherol status on alpha-tocopherol transfer protein expression and its messenger RNA level in rat liver.

Kim H, Arai H, Arita M, Sato Y, Ogihara T, Inoue K, Mino M, *Tamai H.

Free Rad Res 28   87 - 92 1998

Research paper (scientific journal), Joint Work, Accepted

Localization of alpha-tocopherol transfer protein in rat brain.

Hosomi A, Goto K, Kondo H, Iwatsubo T, Yokota T, Ogawa M, Arita M, Aoki J, *Arai H, Inoue K.

Neurosci Lett 256   159 - 162 1998

Research paper (scientific journal), Joint Work, Accepted

Binding of alpha-tocopherylquinone, an oxidized form of alpha-tocopherol, to glutathione-S-transferase in the liver cytosol.

Arita M, Sato Y, *Arai H, Inoue K.

FEBS Lett 436   424 - 426 1998

Research paper (scientific journal), Joint Work, Accepted

Friedreich-like ataxia with retinis pigmentosa caused by the His101Gln mutation of the alpha-tocopherol transfer protein gene.

*Yokota T, Shiojiri T, Gotoda T, Arita M, Arai H, Ohga T, Kanda T, Suzuki J, Imai T, Matsumoto H, Harino S, Kiyosawa M, Mizusawa H, Inoue K.

Ann Neurol 41   826 - 837 1997

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)

Alpha-tocopherol transfer protein stimulates the secretion of alpha-tocopherol from a cultured liver cell line through a brefeldin A-insensitive pathway.

Arita M, Nomura K, *Arai H, Inoue K.

Proc Natl Acad Sci USA 94   12437 - 12441 1997

Research paper (scientific journal), Joint Work, Accepted

Affinity for alpha-tocopherol transfer protein as a determinant of the biological activities of vitamin E analogs.

Hosomi A, Arita M, Sato Y, Kiyose C, Ueda T, Igarashi O, *Arai H, Inoue K.

FEBS Lett 409   105 - 108 1997

Research paper (scientific journal), Joint Work, Accepted

Age-related changes of alpha-tocopherol transfer protein expression in rat liver.

Kim H, Arai H, Arita M, Sato Y, Ogihara T, Tamai H, Inoue K, *Mino M.

J Nutr Sci Vitaminol 42   11 - 18 1996

Research paper (scientific journal), Joint Work, Accepted

Human alpha-tocopherol transfer protein: cDNA cloning, expression and chromosomal localization.

Arita M, Sato Y, Miyata A, Tanabe T, Takahashi E, Kayden HJ, Arai H, *Inoue K.

Biochem J 306   437 - 443 1995

Research paper (scientific journal), Joint Work, Accepted

Adult-onset spinocerebellar dysfunction caused by a mutation in the gene for the alpha-tocopherol transfer protein.

Gotoda T, Arita M, Arai H, Inoue K, Yokota T, Fukuo Y, Yazaki Y, *Yamada N.

N Engl J Med 333   1313 - 1318 1995

Research paper (scientific journal), Joint Work, Accepted

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Ataxia with isolated vitamin E deficiency is caused by mutations in the alpha-tocopherol transfer protein.

Ouahchi K, Arita M, Kayden H, Hentati F, Ben Hamida M, Sokol R, Arai H, Inoue K, Mandel JL, *Koenig M.

Nat Genet 9   141 - 145 1995

Research paper (scientific journal), Joint Work, Accepted

• Access to Document (DOI)