Soga, Tomoyoshi



Graduate School of Media and Governance (Shonan Fujisawa)



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Message from the Faculty Member 【 Display / hide

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

Career 【 Display / hide

  • 1984.04

    Application Chemist, Yokogawa Corp.

  • 1992.04

    Yokogawa Analytical Systems Inc.

  • 2001.04

    University of the Ryukyus, Visiting Professor

  • 2001.04

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07

    Human Metabolome Technologies Inc., Director

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Academic Background 【 Display / hide

  • 1980.04

    Keio University, Faculty of Engineering, Applied Chemistry

    University, Graduated

Academic Degrees 【 Display / hide

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03


Research Themes 【 Display / hide

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質(メタボローム)の測定法, 



Books 【 Display / hide

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23), Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page: 307-313

     View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page: 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page: 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page: 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page: 134-160


    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page: 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page: 113-122

     View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide

  • Relationship between [18 F]FDG PET/CT and metabolomics in patients with colorectal cancer

    Imajo, M., Norikane, T., Yamamoto, Y., Maeda, Y., Saitoh, K., Kato, K., Soga, T., Okano, K., Nishiyama, Y.

    Metabolomics 18 ( 11 ) 91 2022.11

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Introduction: Advances in metabolomics have significantly improved cancer detection, diagnosis, treatment, and prognosis.

    Objectives: To investigate the relationship between metabolic tumor volume (MTV) using 2-deoxy-2-[18F]fluoro-D-glucose (FDG) positron emission tomography (PET)/ computed tomography (CT) and metabolomics data in patients with colorectal cancer (CRC).

    Methods: The metabolome in tumor tissues was analyzed using capillary electrophoresis time-of-flight mass spectrometry in 33 patients with newly diagnosed CRC who underwent FDG PET/CT before treatment and had tumor tissue post-surgery. Based on the FDG PET data, MTV was calculated and was dichotomized according to the median value, and tumors were divided into low-MTV and high-MTV tumors. Metabolomics data were compared between the low-MTV and high-MTV tumors.

    Results: The levels of most glycolysis-related metabolites were not different between low-MTV and high-MTV tumors. The level of component of the initial part of the tricarboxylic acid (TCA) cycle, citrate, was significantly lower in the high-MTV tumor than in the low-MTV tumor. The TCA intermediate succinate level was significantly higher in the high-MTV tumor than in the low-MTV tumor. In contrast, the TCA intermediate fumarate level was significantly lower in the high-MTV tumor than in the low-MTV tumor. The levels of many amino acids were significantly higher in the high-MTV tumor than in the low-MTV tumor.

    Conclusions: Although preliminary, these results suggest that tumors with high FDG metabolism in CRC may obtain more energy by using a reverse reaction of the TCA cycle and amino-acid metabolism. However, further research is required to clarify this relationship.

    Keywords: Colorectal cancer; FDG; Metabolome; PET/CT.

  • Comprehensive metabolome analysis of intracellular metabolites in cultured cells

    Kudo, R., Igarashi, K., Soga, T., Ishikawa, T., Saito, Y.

    STAR Protoc. 3 ( 3 ) 101531 2022.09

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types.

  • Development of Fluorophosphoramidate as a Biocompatibly Transformable Functional Group and its Application as a Phosphate Prodrug for Nucleoside Analogs

    Yoshida, Y., Zheng, T., Tanabe, W., Tomoike, F., Hashiya, F., Suzuki, T., Hirota, S., Saiki, Y., Horii, A., Hirayama, A., Soga, T., Kimura, Y., Abe, H.

    ChemMedChem 17 ( 17 ) e202200188 2022.09

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Synthetic phosphate-derived functional groups are important for controlling the function of bioactive molecules in vivo. Herein we describe the development of a new type of biocompatible phosphate analog, a fluorophosphoramidate (FPA) functional group that has characteristic P-F and P-N bonds. We found that FPA with a primary amino group was relatively unstable in aqueous solution and was converted to a monophosphate, while FPA with a secondary amino group was stable. Furthermore, by improving the molecular design of FPA, we developed a reaction in which a secondary amino group is converted to a primary amino group in the intracellular environment and clarified that the FPA group functions as a phosphate prodrug of nucleoside. Various FPA-gemcitabine derivatives were synthesized and their toxicity to cancer cells were evaluated. One of the FPA-gemcitabine derivatives showed superior toxicity compared with gemcitabine and its ProTide prodrug, which methodology is widely used in various nucleoside analogs, including anti-cancer and anti-virus drugs.

  • Brown adipose tissue dysfunction promotes heart failure via a trimethylamine N-oxide-dependent mechanism

    Yoshida, Y., Shimizu, I., Shimada, A., Nakahara, K., Yanagisawa, S., Kubo, M., Fukuda, S., Ishii, C., Yamamoto, H., Ishikawa, T., Kano, K., Aoki, J., Katsuumi, G., Suda, M., Ozaki, K., Yoshida, Y., Okuda, S., Ohta, S., Okamoto, S., Minokoshi, Y., Oda, K., Sasaoka, T., Abe, M., Sakimura, K., Kubota, Y., Yoshimura, N., Kajimura, S., Zuriaga, M., Walsh, K., Soga, T., Minamino, T.

    Sci. Rep. 12   14883 2022.09

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Low body temperature predicts a poor outcome in patients with heart failure, but the underlying pathological mechanisms and implications are largely unknown. Brown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. While these reports suggest a potential link between BAT and heart failure, the potential role of BAT dysfunction in heart failure has not been investigated. Here, we demonstrate that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. Thoracic aortic constriction (TAC) or myocardial infarction (MI) reduced the thermogenic capacity of BAT in mice, leading to significant reduction of body temperature with cold exposure. BAT became hypoxic with TAC or MI, and hypoxic stress induced apoptosis of brown adipocytes. Enhancement of BAT function improved thermogenesis and cardiac function in TAC mice. Conversely, systolic function was impaired in a mouse model of genetic BAT dysfunction, in association with a low survival rate after TAC. Metabolomic analysis showed that reduced BAT thermogenesis was associated with elevation of plasma trimethylamine N-oxide (TMAO) levels. Administration of TMAO to mice led to significant reduction of phosphocreatine and ATP levels in cardiac tissue via suppression of mitochondrial complex IV activity. Genetic or pharmacological inhibition of flavin-containing monooxygenase reduced the plasma TMAO level in mice, and improved cardiac dysfunction in animals with left ventricular pressure overload. In patients with dilated cardiomyopathy, body temperature was low along with elevation of plasma choline and TMAO levels. These results suggest that maintenance of BAT homeostasis and reducing TMAO production could be potential next-generation therapies for heart failure.

  • Salivary metabolomics with machine learning for colorectal cancer detection

    Kuwabara, H., Katsumata, K., Iwabuthi, A., Udo, R., Tago, T., Kasahara, K., Mazaki, J., Enomoto, M., Ishizaki, T., Soya, R., Kaneko, M., Ota, S., Enomoto, A., Soga, T., Tomita, M., Sunamura, M., Tsuchida, A., Sugimoto, M., Nagakawa, Y.

    Cancer Sci. 113 ( 9 ) 3234 - 3243 2022.09

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    As the worldwide prevalence of colorectal cancer (CRC) increases, it is vital to reduce its morbidity and mortality through early detection. Saliva-based tests are an ideal noninvasive tool for CRC detection. Here, we explored and validated salivary biomarkers to distinguish patients with CRC from those with adenoma (AD) and healthy controls (HC). Saliva samples were collected from patients with CRC, AD, and HC. Untargeted salivary hydrophilic metabolite profiling was conducted using capillary electrophoresis–mass spectrometry and liquid chromatography–mass spectrometry. An alternative decision tree (ADTree)-based machine learning (ML) method was used to assess the discrimination abilities of the quantified metabolites. A total of 2,602 unstimulated saliva samples were collected from subjects with CRC (n = 231), AD (n = 54), and HC (n = 2,317). Data were randomly divided into training (n = 1,301) and validation datasets (n = 1,301). The clustering analysis showed a clear consistency of aberrant metabolites between the two groups. The ADTree model was optimized through cross-validation (CV) using the training dataset, and the developed model was validated using the validation dataset. The model discriminating CRC + AD from HC showed area under the receiver operating characteristic curves (AUC) of 0.860 (95% confidence interval [CI]: 0.828-0.891) for CV and 0.870 (95% CI: 0.837-0.903) for the validation dataset. The other model discriminating CRC from AD + HC showed an AUC of 0.879 (95% CI: 0.851-0.907) and 0.870 (95% CI: 0.838 - 0.902), respectively. Salivary metabolomics combined with ML demonstrated high accuracy and versatility in detecting CRC.

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • メタボロミクスによるがん幹細胞の代謝研究


    (別冊 医学のあゆみ) 治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    (別冊・医学のあゆみ) メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

  • メタボロミクスによるがん幹細胞の代謝研究

    Kitajima, S., Soga, T.

    Journal of Clinical and Experimental Medicine(IGAKU NO AYUMI) (Ishiyaku Pub,Inc.)  273 ( 5 ) 469 - 473 2020.05

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    Journal of Clinical and Experimental Medicine(IGAKU NO AYUMI) (Ishiyaku Pub,Inc.)  270 ( 5 ) 377 - - 2019.08

    Article, review, commentary, editorial, etc. (scientific journal)

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Presentations 【 Display / hide

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 


    Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism


    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 


    Oral presentation (invited, special)

  • Onco-metabolites and cancer specific metabolic pathways


    American Association for Cancer Research Annual Meeting 2017, AACR2017, 


    Oral presentation (invited, special)

  • What Causes Altered Metabolism in Colon Cancer Cells


    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 


    Oral presentation (invited, special)

  • CE-MS Metabolomics and Application to Cancer cell Metabolism


    11th International Conference of the Metabolomics Society, Metabolomics 2015, (Hyatt Regency San Francisco Airport, Burlingame, California, USA) , 


    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems


    Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned research, Coinvestigator(s)

  • Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer


    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned research, Coinvestigator(s)

  • Mitochondrial Medicine


    Japan Agency for Medical Research and Development(AMED), Moonshot Research and Development Program, Abe, Takaaki, Commissioned research, Coinvestigator(s)

  • Drug development targeting metabolic vulnerability of SUCLA2 deletion


    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , TAKAHASHI, Chiaki, Commissioned research, Coinvestigator(s)

  • Development of risk prediction models for mild cognitive decline and frailty using metabolomics in a population-based cohort


    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Takebayashi, Toru, Research grant, Coinvestigator(s)

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Works 【 Display / hide

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師



    Other, Joint

  • 日経BP社主催バイオファイナンスギルド メタボローム講座



    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析




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Intellectual Property Rights, etc. 【 Display / hide

  • カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

    Date applied: 特願平01-272206  1989.10 

    Date announced: 特開平03-134561  1991.06 

    Date issued: 特許第2833058号  1998.10

    Patent, Single

  • 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

    Date applied: 特願平08-143048  1996.06 

    Date announced: 特開平09-325130  1997.12 

    Date issued: 特許第2912232号  1999.04

    Patent, Single

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

    Date applied: 特願平10-145244  1998.05 

    Date announced: 特開平11-337524  1999.12 

    Date issued: 特許第3038184号  2000.02

    Patent, Single

  • 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

    Date applied: 特願2001-224341  2001.07 

    Date announced: 特開2003-035698  2003.02 

    Date issued: 特許第3341765号  2002.08

    Patent, Single

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    Date applied: 特願2004-245728  2004.08 

    Date announced: 特開2006-064472  2006.03 

    Date issued: 特許第3871689号  2006.10

    Patent, Joint

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Awards 【 Display / hide

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award: Keio commendation etc.

  • 第7回酸化ストレスと肝研究会 奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award: Award from Japanese society, conference, symposium, etc.

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  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award: Other

  • The Minister of Education, Culture, Sports, Science& Technology Award

    SOGA Tomoyoshi, TOMITA Masaru, 2007.04, Minister of Education, Culture, Sports, Science& Technology, メタボローム解析技術に基づくバイオマーカーの探索研究

    Type of Award: Other

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Courses Taught 【 Display / hide











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