Soga, Tomoyoshi

写真a

Affiliation

Research Centers and Institutes, Human Biology-Microbiome-Quantum Research Center (Mita)

Position

Project Professor (Non-tenured)

Related Websites

External Links
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Message from the Faculty Member 【 Display / hide 】

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

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Other Disclosed Information 【 Display / hide 】

  • Metabolomics, Analytical Chemistry

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Career 【 Display / hide 】

  • 1984.04
    -
    1992.03

    Application Chemist, Yokogawa Corp.

  • 1992.04
    -
    2001.03

    Yokogawa Analytical Systems Inc.

  • 2001.04
    -
    2002.03

    University of the Ryukyus, Visiting Professor

  • 2001.04
    -
    2005.03

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07
    -
    2010.03

    Human Metabolome Technologies Inc., Director

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Academic Background 【 Display / hide 】

  • 1980.04
    -
    1984.03

    Keio University, Faculty of Engineering, Applied Chemistry

    University, Graduated

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Academic Degrees 【 Display / hide 】

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03

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Research Themes 【 Display / hide 】

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質（メタボローム）の測定法, 

    　

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Books 【 Display / hide 】

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23）, Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page： 307-313

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page： 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page： 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page： 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page： 134-160

  • ONCO-METABOLOMICS; A NEW CLUE TO UNDERSTAND CARCINOGENESIS, CANCER BIOLOGY AND TO DEVELOP NOVEL DIAGNOSTICS AND THERAPEUTICS

    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page： 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page： 113-122

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide 】

  • Decreased Fecal Nicotinamide and Increased Bacterial Nicotinamidase Gene Expression in Ulcerative Colitis Patients

    Aoyama, K., Yamamura, R., Katsurada, T., Shimizu, T., Takahashi, D., Kondo, E., Iwasaki, N., Tamakoshi, A., Soga, T., Fukuda, S., Sonoshita, M., Sakamoto, N.

    Inflamm. Bowel Dis. in press   izaf092 2025.05

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Background/objective: Ulcerative colitis (UC) is significantly linked with gut microbiota, which is essential for maintaining gut health. Their metabolites mitigate gut inflammation and bolster barrier function. Among these metabolites, we focused on vitamin B3, which has been reported to improve the pathogenesis of UC in mice. This study aimed to compare fecal vitamin B3 and gut microbiota between non-UC and UC patients.

    Methods: We assessed fecal metabolites and gut microbiota in 71 UC patients (UC group) and 72 non-UC patients (non-UC group) matched by sex and age in 10-year intervals. Fecal samples were collected and metabolites were analyzed using capillary electrophoresis time-of-flight mass spectrometry. Bacterial DNA was extracted for 16S rRNA gene sequencing. We analyzed fecal nicotinamide levels and gut microbiota composition, employing statistical adjustments for confounding factors.

    Results: We found that the UC group exhibited significantly lower fecal nicotinamide levels and α-diversity (Shannon index) compared to the non-UC group. The relative abundance of bacterial genera such as Treponema, UCG-002, and Fusicatenibacter was decreased, while Sellimonas, Fournierella, and Oscillospira were increased in the UC group. Moreover, a negative correlation was observed between Sellimonas abundance and fecal nicotinamide levels in the UC group. Additionally, the UC group showed higher expression of a bacterial gene encoding nicotinamidase compared to the non-UC group.

    Conclusions: These findings suggest that gut microbiota dysbiosis contributes to reduced vitamin B3 metabolism in UC patients. The study highlights the potential of replenishing vitamin B3 metabolic pathways as a novel therapeutic approach for UC treatment.

    Keywords: gut microbiota; inflammatory bowel disease; nicotinamide; ulcerative colitis; vitamin B3.

  • Dietary amino acids regulate Salmonella colonization via microbiota-dependent mechanisms in the mouse gut

    Pickard, J., Porwollik, S., Caballero-Flores, G., Caruso, R., Fukuda, S., Soga, T., Inohara, N., McClelland, M., Nunez, G.

    Nat. Commun.  16 ( 1 ) 4225 2025.05

    Research paper (scientific journal), Joint Work

    　View Summary

    The gut microbiota confers host protection against pathogen colonization early after infection. Several mechanisms underlying the protection have been described, but the contributions of nutrient competition versus direct inhibition are controversial. Using an ex vivo model of Salmonella growth in the mouse cecum with its indigenous microbes, we find that nutrient limitation and typical inhibitory factors alone cannot prevent pathogen growth. However, the addition of certain amino acids markedly reverses the microbiota's ability to suppress pathogen growth. Enhanced Salmonella colonization after antibiotic treatment is ablated by exclusion of dietary protein, which requires the presence of the microbiota. Thus, dietary protein and amino acids are important regulators of colonization resistance.

  • MOB1 deletion in murine mature adipocytes ameliorates obesity and diabetes

    Nishio, M., Yamaguchi, K., Otani, J., Yuguchi, K., Kohno, D., Sasaki, T., Kitamura, T., Shinohara, M., Soga, T., Kawamura, K., Sasaki, A.T., Oshima, M., Hikasa, H., Woo, M., Sasaki, T., Nishina, H., Nakao, K., Maehama, T., Suzuki, A.

    Proc Natl Acad Sci USA 122 ( 17 ) e2424741122 2025.04

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  00278424

    　View Summary

    There is currently a global epidemic of obesity and obesity-related diseases such as type 2 diabetes due to decreased physical activity, excessive food intake, and/or genetic predisposition. The Hippo-YAP1 pathway has attracted attention as a potential therapeutic target because YAP1/TAZ activation in murine immature adipocytes in vitro suppresses their differentiation by inhibiting PPARγ activity. However, the role of YAP1 activation in mature adipocytes in vivo remains unclear. MOB1, whose expression is increased in obesity, is the hub of the Hippo core molecule complex and negatively regulates YAP1/TAZ activation. Therefore, we generated aMob1DKO mutant mice, which feature deficiency of Mob1a/b specifically in mature adipocytes. Compared to controls, aMob1DKO mice subjected to a high-fat diet showed beneficial changes consistent with resistance to diet-induced obesity. The mutants exhibited increases in basal lipolysis, "beiging," and energy expenditure, as well as suppression of ROS production and inflammation in white adipose tissue. Insulin sensitivity and glucose tolerance were improved, and ectopic fat accumulation was reduced. Most of these changes were dependent on the YAP1 activation observed in mature white adipose tissue of aMob1DKO mice. FGF21, which improves lipid metabolism, was upregulated directly via YAP1 activation, and many of the phenotypes seen in aMob1DKO mice were also dependent on FGF21. Thus, the aMob1DKO mouse is an interesting model for the study of the metabolic effects of diet-induced obesity and protection against diabetes. Our work suggests that a YAP1-FGF21 axis exists in adipocytes that may be a potential therapeutic target for obesity.

  • Genome-wide association study of plasma amino acids and Mendelian randomization for cardiometabolic traits

    Toki, R., Fushiki, S., Kojima, S., Sutoh, Y., Otsuka-Yamasaki, Y., Harada, S., Iida, M., Hirata, A., Miyagawa, N., Matsumoto, M., Edagawa, S., Miyake, A., Kuwabara, K., Hirayama, A., Sugimoto, M., Sato, A., Amano, K., Soga, T., Tomita, M., Arakawa, K., Kinoshita, K., Sakurai-Yageta, M., Tamiya, G., Ohmomo, H., Shimizu, A., Okamura, T., Takebayashi, T.

    Sci. Rep. 15 ( 1 ) 14569 2025.04

    Research paper (scientific journal), Accepted

    　View Summary

    Plasma amino acids (AAs) have emerged as promising biomarkers for metabolic disorders, yet their causality remains unclear. We aimed to investigate the genetic determinants of AA levels in a cohort of 10,333 individuals and their causal effects on cardiometabolic traits using Mendelian randomization (MR). Plasma levels of 20 AAs were quantified using capillary electrophoresis mass spectrometry. Genome-wide association studies were conducted using BOLT-LMM and heritability estimation via LDSC analysis. Causal effects of AAs on 11 cardiometabolic traits were examined using two-sample MR analyses. We identified 85 locus-metabolite associations across 43 genes for 18 AAs, including 44 novel loci linked to metabolic genes. Heritability for AAs was estimated at 16%. MR analysis demonstrated cystine to positively associate with systolic blood pressure (SBP) (β = 0.056, SE = 0.010), while serine indicated protective effects on SBP (β = - 0.040, SE = 0.011), diastolic BP (β = - 0.044, SE = 0.010), and coronary artery disease (odds ratio 0.888, SE = 0.028). We identified potentially novel genetic loci associated with AA levels and demonstrated robust causal associations between several AAs and cardiometabolic traits. These findings reinforce the importance of AAs as potential biomarkers and therapeutic targets in cardiometabolic health.

  • Structural robustness and temporal vulnerability of the starvation-responsive metabolic network in healthy and obese mouse liver

    Morita, K., Hatano, A., Kokaji, T., Sugimoto, H., Tsuchiya, T., Ozaki, H., Egami, R., Li, D., Terakawa, A., Ohno, S., Inoue, H., Inaba, Y., Suzuki, Y., Matsumoto, M., Takahashi, M., Izumi, Y., Bamba, T., Hirayama, A., Soga, T., Kuroda, Shinya

    Sci. Signal.  18 ( 883 ) eads2547 2025.04

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  19450877

    　View Summary

    Adaptation to starvation is a multimolecular and temporally ordered process. We sought to elucidate how the healthy liver regulates various molecules in a temporally ordered manner during starvation and how obesity disrupts this process. We used multiomic data collected from the plasma and livers of wild-type and leptin-deficient obese (ob/ob) mice at multiple time points during starvation to construct a starvation-responsive metabolic network that included responsive molecules and their regulatory relationships. Analysis of the network structure showed that in wild-type mice, the key molecules for energy homeostasis, ATP and AMP, acted as hub molecules to regulate various metabolic reactions in the network. Although neither ATP nor AMP was responsive to starvation in ob/ob mice, the structural properties of the network were maintained. In wild-type mice, the molecules in the network were temporally ordered through metabolic processes coordinated by hub molecules, including ATP and AMP, and were positively or negatively coregulated. By contrast, both temporal order and coregulation were disrupted in ob/ob mice. These results suggest that the metabolic network that responds to starvation was structurally robust but temporally disrupted by the obesity-associated loss of responsiveness of the hub molecules. In addition, we propose how obesity alters the response to intermittent fasting.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 腫瘍における分岐鎖アミノ酸トランスポーターの役割

    Saito, Y., Soga, T.

    Experimental Medicine (YODOSHA)  40 ( 14 ) 2239 - 2244 2022.08

    Article, review, commentary, editorial, etc. (other), Joint Work

  • アミノ酸トランスポーターを標的とした個別化医療

    Precision Medicine 4 ( 13 ) 18 - 22 2021.11

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボロミクスによるがん幹細胞の代謝研究

    北島正二朗、曽我朋義

    （別冊 医学のあゆみ）　治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    （別冊・医学のあゆみ） メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

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Presentations 【 Display / hide 】

  • Development of CE-MS Metabolomics and its Application in Cancer

    Tomoyoshi Soga

    Metabolomics2024, 20th Annual Confereence of the Metabolomics Society (Osaka) , 

    2024.06

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 

    2018.10
    , 

    Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

    SOGA TOMOYOSHI

    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 

    2017.11
    , 

    Oral presentation (invited, special)

  • Onco-metabolites and cancer specific metabolic pathways

    SOGA TOMOYOSHI

    American Association for Cancer Research Annual Meeting 2017, AACR2017, 

    2017.04
    , 

    Oral presentation (invited, special)

  • What Causes Altered Metabolism in Colon Cancer Cells

    SOGA TOMOYOSHI

    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 

    2015.11
    , 

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Study of therapeutic approaches to SUCLA2-deleted cancers

    2024.05
    -
    2025.03

    Japan Agency for Medical Research and Development(AMED), Project for Promotion of Cancer Research and Therapeutic Evolution（P-PROMOTE）, Takahashi, Chiaki, Commissioned ｒesearch, Coinvestigator(s)

  • Transomic analysis of hybernation and torpor Planned Research

    2023.06
    -
    2025.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Transformative Research Areas (A), Kuroda, Shinya, Research grant, Coinvestigator(s)

  • 大腸がんにおけるオンコメタボライト・L-2HGの分子基盤の解明

    2022.06
    -
    2025.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Scientific Research (C), Tabata, Sho, Other, Coinvestigator(s)

  • Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems

    2021.10
    -
    2025.03

    Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned ｒesearch, Coinvestigator(s)

  • Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer

    2021.05
    -
    2023.03

    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned ｒesearch, Coinvestigator(s)

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Works 【 Display / hide 】

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

    SOGA TOMOYOSHI

    2004.12
    , 

    Other, Joint

  • 日経BP社主催バイオファイナンスギルド　メタボローム講座

    SOGA TOMOYOSHI

    2004.08
    , 

    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析

    そがともよし

    2001.10
    -
    Present
    , 

    Other

    　View Details

    キャピラリー電気泳動法の装置、測定法の原理および様々な測定例を解説した

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Intellectual Property Rights, etc. 【 Display / hide 】

  • カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

    Date applied： 特願平01-272206  1989.10 

    Date announced： 特開平03-134561  1991.06 

    Date issued： 特許第2833058号  1998.10

    Patent, Single

  • 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

    Date applied： 特願平08-143048  1996.06 

    Date announced： 特開平09-325130  1997.12 

    Date issued： 特許第2912232号  1999.04

    Patent, Single

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

    Date applied： 特願平10-145244  1998.05 

    Date announced： 特開平11-337524  1999.12 

    Date issued： 特許第3038184号  2000.02

    Patent, Single

  • 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

    Date applied： 特願2001-224341  2001.07 

    Date announced： 特開2003-035698  2003.02 

    Date issued： 特許第3341765号  2002.08

    Patent, Single

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    Date applied： 特願2004-245728  2004.08 

    Date announced： 特開2006-064472  2006.03 

    Date issued： 特許第3871689号  2006.10

    Patent, Joint

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Awards 【 Display / hide 】

  • Fukuzawa Award

    Soga, T., 2022.11, Keio Univ., メタボローム（細胞内全代謝物質）解析技術の開発と実用化

    Type of Award： Keio commendation etc.

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Keio commendation etc.

  • 第7回酸化ストレスと肝研究会　奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award： Award from Japanese society, conference, symposium, etc.

    　View Description

    第7回酸化ストレスと肝研究会における研究発表による

  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award： Other

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Courses Taught 【 Display / hide 】

  • SEMINAR B

    2024

  • METABOLOMICS

    2024

  • METABOLOME ANALYSIS LABORATORY PRACTICE

    2024

  • MASTER SEMINAR

    2024

  • INDEPENDENT RESEARCH

    2024

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