Soga, Tomoyoshi

写真a

Affiliation

Faculty of Environment and Information Studies （Shonan Fujisawa）

Position

Professor

Related Websites

External Links
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Message from the Faculty Member 【 Display / hide 】

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

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Career 【 Display / hide 】

  • 1984.04
    -
    1992.03

    Application Chemist, Yokogawa Corp.

  • 1992.04
    -
    2001.03

    Yokogawa Analytical Systems Inc.

  • 2001.04
    -
    2002.03

    University of the Ryukyus, Visiting Professor

  • 2001.04
    -
    2005.03

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07
    -
    2010.03

    Human Metabolome Technologies Inc., Director

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Academic Degrees 【 Display / hide 】

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03

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Research Themes 【 Display / hide 】

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質（メタボローム）の測定法, 

    　

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Books 【 Display / hide 】

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23）, Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page： 307-313

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page： 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page： 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page： 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page： 134-160

  • ONCO-METABOLOMICS; A NEW CLUE TO UNDERSTAND CARCINOGENESIS, CANCER BIOLOGY AND TO DEVELOP NOVEL DIAGNOSTICS AND THERAPEUTICS

    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page： 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page： 113-122

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide 】

  • CE-MS-based Identification of Uremic Solutes Specific to Hemodialysis Patients

    Akiyama, Y., Kikuchi, K., Toyohara, T., Mishima, E., Suzuki, C., Suzuki, T., Nakayama, M., Tomioka, Y., Soga, T., Abe, T.

    Toxins 13 （ 5 ） 324 2021.04

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Uremic toxins are suggested to be involved in the pathophysiology of hemodialysis (HD) patients. However, the profile of uremic solutes in HD patients has not been fully elucidated. In this study using capillary electrophoresis mass spectrometry (CE-MS), we comprehensively quantified the serum concentrations of 122 ionic solutes before and after HD in 11 patients. In addition, we compared the results with those in non-HD patients with chronic kidney disease (CKD) to identify HD patient-specific solutes. We identified 38 solutes whose concentrations were higher in pre-HD than in CKD stage G5. Ten solutes among them did not significantly accumulate in non-HD CKD patients, suggesting that these solutes accumulate specifically in HD patients. We also identified 23 solutes whose concentrations were lower in both pre- and post-HD than in CKD stage G5. The serum levels of 14 solutes among them were not affected by renal function in non-HD patients, suggesting that these solutes tend to be lost specifically in HD patients. Our data demonstrate that HD patients have a markedly different profile of serum uremic solute levels compared to that in non-HD CKD patients. The solutes identified in our study may contribute to the pathophysiology of HD patients.

  • A Metabolomic Profile Predictive of New Osteoporosis or Sarcopenia Development

    Miyamoto, K., Hirayama, A., Sato, Y., Ikeda, S., Maruyama, M., Soga, T., Tomita, M., Nakamura, M., Matsumoto, M., Yoshimura, N., Miyamoto, T.

    Metabolites 11 （ 5 ） 278 2021.04

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    The increasing number of patients with osteoporosis and sarcopenia is a global concern among countries with progressively aging societies. The high medical costs of treating those patients suggest that prevention rather than treatment is preferable. We enrolled 729 subjects who attended both the second and third surveys of the Research on Osteoarthritis/Osteoporosis Against Disability (ROAD) study. Blood samples were collected from subjects at the second survey, and then a comprehensive metabolomic analysis was performed. It was found that 35 had newly developed osteoporosis at the third survey performed four years later, and 39 were newly diagnosed with sarcopenia at the third survey. In the second survey, we found that serum Gly levels were significantly higher even after adjustment for age, sex, and BMI in subjects with newly developed osteoporosis relative to those who remained osteoporosis-negative during the four-year follow-up. We also show that serum taurine levels were significantly lower at the second survey, even after adjustment for age, sex, and BMI in subjects with newly developed sarcopenia during the four-year follow-up compared with those not diagnosed with sarcopenia at the second or third surveys. Though our sample size and odds ratios were small, increased Gly and decreased taurine levels were found to be predictive of new development of osteoporosis and sarcopenia, respectively, within four years.

  • Methionine restriction breaks obligatory coupling of cell proliferation and death by an oncogene Src in Drosophila

    Nishida, H., Okada, M., Yang, L., Takano, T., Tabata, S., Soga, T., Ho, D.M., Chung, J., Minami, Y., Yoo, S.K.

    eLife  10   e59809 2021.04

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Oncogenes often promote cell death as well as proliferation. How oncogenes drive these diametrically opposed phenomena remains to be solved. A key question is whether cell death occurs as a response to aberrant proliferation signals or through a proliferation-independent mechanism. Here, we reveal that Src, the first identified oncogene, simultaneously drives cell proliferation and death in an obligatorily coupled manner through parallel MAPK pathways. The two MAPK pathways diverge from a lynchpin protein Slpr. A MAPK p38 drives proliferation whereas another MAPK JNK drives apoptosis independently of proliferation signals. Src-p38-induced proliferation is regulated by methionine-mediated Tor signaling. Reduction of dietary methionine uncouples the obligatory coupling of cell proliferation and death, suppressing tumorigenesis and tumor-induced lethality. Our findings provide an insight into how cells evolved to have a fail-safe mechanism that thwarts tumorigenesis by the oncogene Src. We also exemplify a diet-based approach to circumvent oncogenesis by exploiting the fail-safe mechanism.

  • Reliability of urinary charged metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry

    Ishibashi, Y., Harada, S., Takeuchi, A., Iida, M., Kurihara, A., Kato, S., Kuwabara, K., Hirata, A., Shibuki, T., Okamura, T., Sugiyama, D., Sato, A, Amano, K., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Takebayashi, T

    Sci. Rep  11 （ 1 ） 7407 2021.04

    Research paper (scientific journal), Accepted

    　View Summary

    Currently, large-scale cohort studies for metabolome analysis have been launched globally. However, only a few studies have evaluated the reliability of urinary metabolome analysis. This study aimed to establish the reliability of urinary metabolomic profiling in cohort studies. In the Tsuruoka Metabolomics Cohort Study, 123 charged metabolites were identified and routinely quantified using capillary electrophoresis-mass spectrometry (CE-MS). We evaluated approximately 750 quality control (QC) samples and 6,720 participants' spot urine samples. We calculated inter- and intra-batch coefficients of variation in the QC and participant samples and technical intraclass correlation coefficients (ICC). A correlation of metabolite concentrations between spot and 24-h urine samples obtained from 32 sub-cohort participants was also evaluated. The coefficient of variation (CV) was less than 20% for 87 metabolites (70.7%) and 20-30% for 19 metabolites (15.4%) in the QC samples. There was less than 20% inter-batch CV for 106 metabolites (86.2%). Most urinary metabolites would have reliability for measurement. The 96 metabolites (78.0%) was above 0.75 for the estimated ICC, and those might be useful for epidemiological analysis. Among individuals, the Pearson correlation coefficient of 24-h and spot urine was more than 70% for 59 of the 99 metabolites. These results show that the profiling of charged metabolites using CE-MS in morning spot human urine is suitable for epidemiological metabolomics studies.

  • Metabolomic Analysis of Small Extracellular Vesicles Derived from Pancreatic Cancer Cells Cultured under Normoxia and Hypoxia

    Hayasaka, R., Tabata, S., Hasebe, M., Ikeda, S., Ohnuma, S., Mori, M., Soga, T., Tomita, M., Hirayama, A.

    Metabolites 11 （ 4 ） 215 2021.04

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Extracellular vesicles (EVs) released from cancer cells contribute to various malignant phenotypes of cancer, including metastasis, cachexia, and angiogenesis. Although DNA, mRNAs, miRNAs, and proteins contained in EVs have been extensively studied, the function of metabolites in EVs remains unclear. In this study, we performed a comprehensive metabolomic analysis of pancreatic cancer cells, PANC-1, cultured under different oxygen concentrations, and small EVs (sEVs) released from them, considering the fact that hypoxia contributes to the malignant behavior of cells in pancreatic cancer, which is a poorly diagnosed cancer. sEVs were collected by ultracentrifugation, and hydrophilic metabolites were analyzed using capillary ion chromatography-mass spectrometry and liquid chromatography-mass spectrometry, and lipids were analyzed by supercritical fluid chromatography-tandem mass spectrometry. A total of 140 hydrophilic metabolites and 494 lipids were detected in sEVs, and their profiles were different from those in cells. In addition, the metabolomic profile of sEVs was observed to change under hypoxic stress, and an increase in metabolites involved in angiogenesis was also detected. We reveal the hallmark of the metabolites contained in sEVs and the effect of tumor hypoxia on their profiles, which may help in understanding EV-mediated cancer malignancy.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • メタボロミクスによるがん幹細胞の代謝研究

    北島正二朗、曽我朋義

    （別冊 医学のあゆみ）　治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Introduction and explanation (scientific journal), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    （別冊・医学のあゆみ） メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

  • メタボロミクスによるがん幹細胞の代謝研究

    Kitajima, S., Soga, T.

    Journal of Clinical and Experimental Medicine（IGAKU NO AYUMI） (Ishiyaku Pub,Inc.)  273 ( 5 ) 469 - 473 2020.05

    Introduction and explanation (scientific journal), Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    Journal of Clinical and Experimental Medicine（IGAKU NO AYUMI） (Ishiyaku Pub,Inc.)  270 ( 5 ) 377 - - 2019.08

    Introduction and explanation (scientific journal)

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Presentations 【 Display / hide 】

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 2018.10, Oral Presentation(guest/special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

    SOGA TOMOYOSHI

    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 2017.11, Oral Presentation(guest/special)

  • Onco-metabolites and cancer specific metabolic pathways

    SOGA TOMOYOSHI

    American Association for Cancer Research Annual Meeting 2017, AACR2017, 2017.04, Oral Presentation(guest/special)

  • What Causes Altered Metabolism in Colon Cancer Cells

    SOGA TOMOYOSHI

    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 2015.11, Oral Presentation(guest/special)

  • CE-MS Metabolomics and Application to Cancer cell Metabolism

    SOGA TOMOYOSHI

    11th International Conference of the Metabolomics Society, Metabolomics 2015, (Hyatt Regency San Francisco Airport, Burlingame, California, USA) , 2015.07, Oral Presentation(guest/special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Drug development targeting metabolic vulnerability of SUCLA2 deletion

    2019.08
    -
    2021.03

    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , TAKAHASHI Chiaki, No Setting, Co-investigator

  • Development of risk prediction models for mild cognitive decline and frailty using metabolomics in a population-based cohort

    2018.04
    -
    2023.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Takebayashi, Toru, Research grant, Co-investigator

  • Metabolomic analysis for the mechanisms of treatment resistance during neoadjuvant chemoradiotherapy in patients with pancreatic adenocarcinoma

    2018.04
    -
    2021.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Okano, Keiichi, No Setting, Co-investigator

  • Elucidation of molecular machineries for the regulation of myeloid leukemia stem cells

    2016.04
    -
    2021.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (S) , Akashi, Koichi, Grant-in-Aid for Scientific Research (S), Research grant, Co-investigator

  • Metabolome-wide metabolic profiling and non-communicable disease prevention in a population-based cohort study

    2015.04
    -
    2018.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Takebayashi, Toru, Research grant, Co-investigator

    　View Remarks

    基盤Aが採択されたことにより、基盤BについてはH29(2017)年度で終了

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Works 【 Display / hide 】

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

    SOGA TOMOYOSHI

    2004.12
    , 

    Other, Joint

  • 日経BP社主催バイオファイナンスギルド　メタボローム講座

    SOGA TOMOYOSHI

    2004.08
    , 

    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析

    そがともよし

    2001.10
    -
    Present
    , 

    Other

    　View Details

    キャピラリー電気泳動法の装置、測定法の原理および様々な測定例を解説した

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Awards 【 Display / hide 】

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Awards of National Conference, Council and Symposium.  Country： 日本

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Keio commendation etc.

  • 第7回酸化ストレスと肝研究会　奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award： Awards of National Conference, Council and Symposium

    　View Description

    第7回酸化ストレスと肝研究会における研究発表による

  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award： Other Awards

  • The Minister of Education, Culture, Sports, Science& Technology Award

    SOGA Tomoyoshi, TOMITA Masaru, 2007.04, Minister of Education, Culture, Sports, Science& Technology, メタボローム解析技術に基づくバイオマーカーの探索研究

    Type of Award： Other Awards

    　View Description

    細胞内に存在する数千種類の代謝物質を一斉に分析する分析技術を世界に先駆けて開発した。代謝物質のほとんどがイオン性を持つことに着目し、イオン性物質に対して高分離能を有するキャピラリー電気泳動（CE）と高選択・高感度検出器である質量分析計（MS）を組み合わせた独創的なものである。数千種類の代謝物質の変化を瞬時に可視化する情報処理技術も開発した。本研究により、急性肝炎時に血中で急増するバイオマーカーと、特定のがん細胞にのみ増加するバイオマーカー候補をすでに発見した。本成果は、バイオマーカーの探索にブレイクスルーをもたらすものであり、医薬分野の進展を促進することが期待される。

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Courses Taught 【 Display / hide 】

  • SEMINAR B

    2021

  • METABOLOMICS

    2021

  • METABOLOME ANALYSIS LABORATORY PRACTICE

    2021

  • MASTER SEMINAR

    2021

  • INDEPENDENT RESEARCH

    2021

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