Soga, Tomoyoshi



Graduate School of Media and Governance (Shonan Fujisawa)



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Message from the Faculty Member 【 Display / hide

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

Career 【 Display / hide

  • 1984.04

    Application Chemist, Yokogawa Corp.

  • 1992.04

    Yokogawa Analytical Systems Inc.

  • 2001.04

    University of the Ryukyus, Visiting Professor

  • 2001.04

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07

    Human Metabolome Technologies Inc., Director

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Academic Background 【 Display / hide

  • 1980.04

    Keio University, Faculty of Engineering, Applied Chemistry

    University, Graduated

Academic Degrees 【 Display / hide

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03


Research Themes 【 Display / hide

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質(メタボローム)の測定法, 



Books 【 Display / hide

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23), Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page: 307-313

     View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page: 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page: 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page: 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page: 134-160


    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page: 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page: 113-122

     View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide

  • Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula

    Suzuki, Y., Hayasaka, R., Hasebe, M., Ikeda, S., Soga, T., Tomita, M., Hirayama, A., Kuroda, H.

    Metabolites 12 ( 6 ) 566 2022.06

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Many previous studies have reported the various proteins specifically secreted as inducers in the dorsal or ventral regions in vertebrate gastrula. However, little is known about the effect on cell fate of small molecules below 1000 Da. We therefore tried to identify small molecules specifically expressed in the dorsal marginal zone (DMZ) or ventral marginal zone (VMZ) in vertebrate gastrula. Small intracellular and secreted molecules were detected using explants and supernatant samples. Hydrophilic metabolites were analyzed by capillary ion chromatography–mass spectrometry and liquid chromatography–mass spectrometry, and lipids were analyzed by supercritical fluid chromatography–tandem mass spectrometry. In total, 190 hydrophilic metabolites and 396 lipids were identified. The DMZ was found to have high amounts of glycolysis- and glutathione metabolism-related metabolites in explants, and the VMZ was richer in purine metabolism-related metabolites. We also discovered some hydrophilic metabolites and lipids differentially contained in the DMZ or VMZ. Our research would contribute to a deeper understanding of the cellular physiology that regulates early embryogenesis.

  • AGE/RAGE axis regulates reversible transition to quiescent states of ALK-rearranged NSCLC and pancreatic cancer cells in monolayer cultures

    Kadonosono, T., Miyamoto, K., Sasaki, S., Matsuo, Y., Kitajima, S., Wang, Q., Endo, M., Nibori, M., Kuchimaru, T., Soga, T., Hirota, K., Kizaka-Kondoh, S.

    Sci. Rep. 12   9886 2022.06

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Cancer recurrence due to tumor cell quiescence after therapy and long-term remission is associated with cancer-related death. Previous studies have used cell models that are unable to return to a proliferative state; thus, the transition between quiescent and proliferative states is not well understood. Here, we report monolayer cancer cell models wherein the human non-small cell lung carcinoma cell line H2228 and pancreatic cancer cell line AsPC-1 can be reversibly induced to a quiescent state under hypoxic and serum-starved (HSS) conditions. Transcriptome and metabolome dual-omics profiles of these cells were compared with those of the human lung adenocarcinoma cell line A549, which was unable to enter a quiescent state under HSS conditions. The quiescence-inducible cells had substantially lower intracellular pyruvate and ATP levels in the quiescent state than in the proliferative state, and their response to sudden demand for energy was dramatically reduced. Furthermore, in quiescence-inducible cells, the transition between quiescent and proliferative states of these cells was regulated by the balance between the proliferation-promoting Ras and Rap1 signaling and the suppressive AGE/RAGE signaling. These cell models elucidate the transition between quiescent and proliferative states, allowing the development of drug-screening systems for quiescent tumor cells.

  • Different types of reactions to E7386 among colorectal cancer patient-derived organoids and corresponding CAFs

    Imai, T., Naruse, M., Ochiai M., Matsumoto K., Ikeda S., Kani, M., Kato, Y., Hirayama, A., Soga, T., Hori, Y., Yokoi, A., Ochiai, A.

    Oncol. Lett.  24 ( 1 ) 221 2022.05

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Colorectal cancer (CRC) harbors genetic alterations in a component of the Wnt signaling pathway in ~90% of cases. In addition, the Wnt signaling pathway has been previously suggested to serve a notable role in the pathophysiology of CRC cells and cancer‑associated fibroblasts (CAFs). In the present study, the possible effects of E7386, a selective inhibitor of the interaction between β‑catenin and the cAMP response element‑binding protein‑binding protein, were evaluated using organoids and the corresponding CAFs derived from patients with CRC. E7386 at 100 nM was revealed to decrease the viability of CRC organoids and CAFs. Analysis of the gene expression profiles revealed marked changes in the expression levels of different types of cancer‑associated genes associated with E7386 concentrations in the organoids and/or CAFs, such as those regulating glucose and amino acid metabolism [phosphoenolpyruvate carboxykinase 2, asparagine synthetase (glutamine‑hydrolyzing), phosphoserine aminotransferase 1 and phosphoglycerate dehydrogenase], stimulation of natural killer cell‑mediated cytotoxicity (UL16‑binding protein 1) and modification of the Wnt/β‑catenin signaling pathway (indicated by very low density lipoprotein receptor). Results of the hydrophilic metabolome analysis in the organoids were consistent with those of the transcriptomic analysis. In vivo experiments used corresponding xenograft models, although changes in volume of tumor tissues could not be observed at 50 mg/kg body weight twice a day for 14 days, results on the protein expression levels partially supported those in the in vitro experiments. In conclusion, different types of reactions, such as alterations in the glucose and amino acid metabolic pathways, stimulation of stress responses and NK‑cell mediated cytotoxicity and components in the Wnt/β‑catenin signaling pathway, to E7386 in the CRC organoids and corresponding CAFs were observed under conditions with decreased cell viability. However, further mechanistic studies to clarify the relationships with Wnt/β‑catenin signaling pathway and these reactions using preclinical models and biomarker studies using clinical human samples are required for verification of the present preclinical biomarkers.

  • Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue

    Yoshida, Y., Shimizu, I., Hsiao, Y.T., Suda, M., Katsuumi, G., Seki, M., Suzuki, Y., Okuda, S., Soga, T., Minamino, T.

    iScience  25 ( 5 ) 104268 2022.05

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Brown adipose tissue (BAT) is a metabolically active organ that contributes to the thermogenic response to cold exposure. In addition, other thermogenic cells termed beige adipocytes are generated in white adipose tissue (WAT) by cold exposure. Although activation of brown/beige adipose tissue is associated with mobilization of both glucose and lipids, few studies have focused on the role of glycolytic enzymes in regulating adipose tissue function. We generated mouse models with specific deletion of the glycolytic enzyme phosphoglycerate mutase 1 (PGAM1) from adipose tissue. Deletion of Pgam1 from both BAT and WAT promoted whitening of BAT with beiging of visceral WAT, whereas deletion of Pgam1 from BAT alone led to whitening of BAT without beiging of WAT. Our results demonstrate a potential role of glycolytic enzymes in beiging of visceral WAT and suggest that PGAM1 would be a novel therapeutic target in obesity and diabetes.

  • Polarity protein SCRIB interacts with SLC3A2 to regulate proliferation and tamoxifen resistance in ER+ breast cancer

    Saito, Y., Matsuda, S., Ohnishi, N., Endo, K., Ashitani, S., Ohishi, M., Ueno, A., Tomita, M., Ueda, K., *Soga, T., *Muthuswamy, S.K

    Commun. Biol.  5 ( 1 ) 403 2022.05

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Estrogen receptor (ER) positive breast cancer represents 75% of all breast cancers in women. Although patients with ER+ cancers receive endocrine therapies, more than 30% develop resistance and succumb to the disease, highlighting the need to understand endocrine resistance. Here we show an unexpected role for the cell polarity protein SCRIB as a tumor-promoter and a regulator of endocrine resistance in ER-positive breast cancer cells. SCRIB expression is induced by estrogen signaling in a MYC-dependent manner. SCRIB interacts with SLC3A2, a heteromeric component of leucine amino acid transporter SLC7A5. SLC3A2 binds to the N-terminus of SCRIB to facilitate the formation of SCRIB/SLC3A2/LLGL2/SLC7A5 quaternary complex required for membrane localization of the amino acid transporter complex. Both SCRIB and SLC3A2 are required for cell proliferation and tamoxifen resistance in ER+ cells identifying a new role for the SCRIB/SLC3A2 complex in ER+ breast cancer.

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • メタボロミクスによるがん幹細胞の代謝研究


    (別冊 医学のあゆみ) 治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    (別冊・医学のあゆみ) メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

  • メタボロミクスによるがん幹細胞の代謝研究

    Kitajima, S., Soga, T.

    Journal of Clinical and Experimental Medicine(IGAKU NO AYUMI) (Ishiyaku Pub,Inc.)  273 ( 5 ) 469 - 473 2020.05

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    Journal of Clinical and Experimental Medicine(IGAKU NO AYUMI) (Ishiyaku Pub,Inc.)  270 ( 5 ) 377 - - 2019.08

    Article, review, commentary, editorial, etc. (scientific journal)

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Presentations 【 Display / hide

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 


    Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism


    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 


    Oral presentation (invited, special)

  • Onco-metabolites and cancer specific metabolic pathways


    American Association for Cancer Research Annual Meeting 2017, AACR2017, 


    Oral presentation (invited, special)

  • What Causes Altered Metabolism in Colon Cancer Cells


    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 


    Oral presentation (invited, special)

  • CE-MS Metabolomics and Application to Cancer cell Metabolism


    11th International Conference of the Metabolomics Society, Metabolomics 2015, (Hyatt Regency San Francisco Airport, Burlingame, California, USA) , 


    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems


    Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned research, Coinvestigator(s)

  • Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer


    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned research, Coinvestigator(s)

  • Mitochondrial Medicine


    Japan Agency for Medical Research and Development(AMED), Moonshot Research and Development Program, Abe, Takaaki, Commissioned research, Coinvestigator(s)

  • Drug development targeting metabolic vulnerability of SUCLA2 deletion


    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , TAKAHASHI, Chiaki, Commissioned research, Coinvestigator(s)

  • Development of risk prediction models for mild cognitive decline and frailty using metabolomics in a population-based cohort


    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Takebayashi, Toru, Research grant, Coinvestigator(s)

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Works 【 Display / hide

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師



    Other, Joint

  • 日経BP社主催バイオファイナンスギルド メタボローム講座



    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析




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Intellectual Property Rights, etc. 【 Display / hide

  • カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

    Date applied: 特願平01-272206  1989.10 

    Date announced: 特開平03-134561  1991.06 

    Date issued: 特許第2833058号  1998.10

    Patent, Single

  • 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

    Date applied: 特願平08-143048  1996.06 

    Date announced: 特開平09-325130  1997.12 

    Date issued: 特許第2912232号  1999.04

    Patent, Single

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

    Date applied: 特願平10-145244  1998.05 

    Date announced: 特開平11-337524  1999.12 

    Date issued: 特許第3038184号  2000.02

    Patent, Single

  • 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

    Date applied: 特願2001-224341  2001.07 

    Date announced: 特開2003-035698  2003.02 

    Date issued: 特許第3341765号  2002.08

    Patent, Single

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    Date applied: 特願2004-245728  2004.08 

    Date announced: 特開2006-064472  2006.03 

    Date issued: 特許第3871689号  2006.10

    Patent, Joint

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Awards 【 Display / hide

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award: Keio commendation etc.

  • 第7回酸化ストレスと肝研究会 奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award: Award from Japanese society, conference, symposium, etc.

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  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award: Other

  • The Minister of Education, Culture, Sports, Science& Technology Award

    SOGA Tomoyoshi, TOMITA Masaru, 2007.04, Minister of Education, Culture, Sports, Science& Technology, メタボローム解析技術に基づくバイオマーカーの探索研究

    Type of Award: Other

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Courses Taught 【 Display / hide











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