KEIO RESEARCHERS INFORMATION SYSTEM

Message from the Faculty Member 【 Display / hide 】

Message from the Faculty Member 【 Display / hide 】

• 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

Other Disclosed Information 【 Display / hide 】

Other Disclosed Information 【 Display / hide 】

• Metabolomics, Analytical Chemistry

Career 【 Display / hide 】

Career 【 Display / hide 】

• 1984.04

  -

  1992.03

  Application Chemist, Yokogawa Corp.

• 1992.04

  -

  2001.03

  Yokogawa Analytical Systems Inc.

• 2001.04

  -

  2002.03

  University of the Ryukyus, Visiting Professor

• 2001.04

  -

  2005.03

  Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

• 2003.07

  -

  2010.03

  Human Metabolome Technologies Inc., Director

display all >>

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 1980.04

  -

  1984.03

  Keio University, Faculty of Engineering, Applied Chemistry

  University, Graduated

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• Ph.D., Toyohashi University of Technology, Dissertation, 2000.03

Research Themes 【 Display / hide 】

Research Themes 【 Display / hide 】

• 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質（メタボローム）の測定法, 

  　

Books 【 Display / hide 】

Books 【 Display / hide 】

• Advances in Microscale Electro-and Liquid Phase-Based Separation Techniques

  El Rassi, Z., Ed. (Soga, T., contribution for Chapter Chapter 9), Elsevier Inc., 2026.03

  Scope: Chapter 9: “Capillary electrophoresis-mass spectrometry”,  Contact page： pp.279-294

  　View Summary

  Capillary electrophoresis-mass spectrometry (CE-MS) has gained a great deal of attention as a powerful tool for the analysis of charged compounds. This approach offers high-resolution, high-sensitivity, and wide versatility. Over the past three decades, several CE-MS methods have been developed and have made significant advances in analytical chemistry. In this chapter, the general strategies and principles of CE-MS methods will be discussed, including CE separation modes, interface techniques, mass spectrometry, new techniques to increase the throughput capability of CE-MS analysis, metabolomics, and even single-cell analysis, which has received much attention in recent years. Finally, general conclusions and perspectives are provided.

  • Access to Document (DOI)

• Amino Acid Analysis : Methods and Protocols

  Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23）, Humana Press, 2019.07

  Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page： 307-313

  　View Summary

  Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

• Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

  Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page： 430

  Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page： 223-234

• Capillary Electrophoresis-Mass Spectrometry for Metabolomics

  Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page： 300

  Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page： 134-160

• ONCO-METABOLOMICS; A NEW CLUE TO UNDERSTAND CARCINOGENESIS, CANCER BIOLOGY AND TO DEVELOP NOVEL DIAGNOSTICS AND THERAPEUTICS

  Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

display all >>

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• S-adenosyl-L-homocysteine triggers an adaptive response that extends lifespan through the YBT1 ABC transporter

  Masumura, K., Kawasaki, S., Tsutsumi, M., Yamashita, H. Kanai, M., Nakajo, H., Yagi, W., Katsunoi, S., Yoneyama, K., Kawano-Kawada, M., Sekito, T., Ogawa, T., Soga, T., Mizunuma, M.

  Proc. Natl. Acad. Sci. USA 123 ( 42 ) e2621286123 2026.10

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  S-adenosyl-L-methionine (SAM) is a fundamental methyl donor; however, the physiological role of its byproduct and competitive inhibitor, S-adenosyl-L-homocysteine (SAH), remains unclear. Here, we show that transient reactive oxygen species (ROS) production triggered by SAH during the early logarithmic phase mimics partial SAH hydrolase inhibition, extending the yeast chronological lifespan (CLS) through a hormetic mechanism. This study reveals that the dual nature of ROS in aging is determined by the specific degree of SAH hydrolase activity. We identified the adenosine 5’-triphosphate-binding cassette (ABC) transporter Ybt1 as a key downstream effector of SAH. Ybt1 localizes to the vacuolar membrane and facilitates the sequestration of SAM from the cytosol into the vacuole. Notably, YBT1 overexpression extends CLS, mimicking SAH effects through an integrated pathway involving target of rapamycin complex 1 downregulation, autophagy, and adenosine 5’-monophosphate-activated protein kinase activation. Our findings establish that SAH acts as a metabolic signal coupling nutrient and energy availability to longevity via Ybt1, defining an adaptive/hormetic response for lifespan regulation in yeast.

  • Access to Document (DOI)

• Pyruvate carboxylase drives therapeutic resistance to KRAS G12C inhibitors in KEAP1-mutant lung cancer

  Akhmetzianova, R., Taniguchi, T., Sasaki, N., Homme, M., Machida, Y., Okahashi, N., Soga, T., Thumkeo, D., Matsuda, F., Takahashi, C., Kitajima, S., Kohno, S.

  Cell Death Dis. online 2026.10

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  In patients with non-small cell lung cancer (NSCLC) treated with KRAS G12C inhibitors, co-occurring mutations in STK11 and KEAP1 are associated with earlier disease progression and poor clinical outcomes. However, the mechanisms underlying primary resistance to KRAS G12C inhibitors remain obscure. Here, we show that loss of KEAP1 function makes tumor cells dependent on pyruvate carboxylase (PC) to sustain nucleotide biosynthesis during KRAS G12C inhibitor treatment. KEAP1 deficiency leads to constitutive activation of NRF2, which promotes PC expression. Metabolic profiling and isotope tracing showed that elevated PC activity channels glucose-derived carbon into the TCA cycle and increases oxaloacetate availability. This supports aspartate synthesis and subsequent de novo pyrimidine biosynthesis. Mechanistically, this metabolic rewiring provides the biosynthetic precursors required to maintain nucleotide pools and allows tumor cells to sustain proliferation during KRAS G12C inhibition. Importantly, in models harboring concurrent STK11 and KEAP1 mutations, genetic ablation of PC restored sensitivity to KRAS G12C inhibition and suppressed tumor growth. Our findings identify PC-mediated anaplerosis as a metabolic vulnerability in STK11 and KEAP1 co-mutant tumors and provide a rationale for targeting PC in combination with KRAS G12C inhibition to overcome primary resistance.

  • Access to Document (DOI)

• Lysophospholipase PNPLA7 is a critical checkpoint of phosphatidylcholine catabolism and turnover for skeletal muscle homeostasis

  Matsumoto, S., Seo, H., Taketomi, Y., Higashi, T., Nagasaki, Y., Sato, H., Kawaguchi, M., Nishito, Y., Park, J., Mizuguchi, K., Hosomi, K., Kunisawa, J., Saito, K., Kato, K., Soga, T., Hirabayashi, T., Ogawa, S., Murakami, M.

  Cell Rep. online 2026.09

  Research paper (scientific journal), Joint Work

  　View Summary

  Current evidence suggests that disturbed phosphatidylcholine (PC) turnover causes skeletal muscle dysfunction. However, the enzyme that converts lysophosphatidylcholine (LPC) to glycerophosphocholine (GPC), an irreversible checkpoint in PC catabolism, in skeletal muscle has remained unidentified. Here we show that PNPLA7, a patatin-like phospholipase isoform, is responsible for this process. Pnpla7 deletion in myoblasts impedes the conversion of LPC to GPC and then to choline. Quadriceps muscles in global Pnpla7-deficient mice display thinner muscle fibers with aberrant mitochondrial morphology, reduced endurance capacity, and decreased expression of genes related to fatty acid β-oxidation, mitochondrial functions, and slow-twitch fibers with age. These abnormalities are preceded by alterations in phospholipid composition, with decreases in docosahexaenoic acid-containing PC and mitochondrial cardiolipin. Moreover, skeletal muscle-specific Pnpla7 deficiency results in muscle phenotypes similar to its global deficiency. Identification of the lysophospholipase PNPLA7 aids further understanding of the role of PC turnover in skeletal muscle homeostasis.

  • Access to Document (DOI)

• he Gibbs free energy landscape based on liver metabolome revealed thermodynamic robustness against fasting and obesity

  Abekawa, T., Ohno, S., Hirayama, A., Soga, T., Kuroda, S.

  iScience 29 ( 10 ) 117618 2026.09

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  Mammalian liver metabolism shifts substantially during fasting, yet the Gibbs free energy change of reaction (ΔrG′), a fundamental thermodynamic quantity that defines reaction driving force and constrains metabolic directionality, remains poorly characterized in intact organs. Here, we mapped the ΔrG′ landscape of mouse liver glucose metabolism during fasting using absolute metabolite concentrations and GLEAM (Gibbs free energy of reaction landscape estimation from metabolome assisted by variance-covariance matrix), a computational method developed here. Despite metabolite fluctuations, ΔrG′ landscapes remained robust, changing only modestly, even for reactions reversing direction between glycolysis and gluconeogenesis. Thermodynamically inferred potential rate-limiting steps were maintained during fasting. Enzyme-cost analysis further suggested a trade-off between thermodynamic robustness and enzyme requirements during glycolytic switching. Obese mouse liver also showed thermodynamic robustness despite metabolic disruption. Our framework provides a novel thermodynamic perspective on intact organ metabolism, revealing preservation of key thermodynamic characteristics across fasting and obesity.

  • Access to Document (DOI)

• Mitochonic acid-5 alleviates amyotrophic lateral sclerosis phenotypes via mitochondrial augmentation

  Oikawa, Y., Luo, Y., Suzuki, N., Kasahara, T., Tongu, Y., Yoshida, Y., Tominari, T., Tanabe, S., Suto, Y., Kashiwagi, H., Saito, S., Ikeda, K., Suzuki, C., Kuranaga, A., Akiyama, T., Morimoto, S., Aoki, Y., Marumatsu, R., Soga, T., Aoki, M., Okano, H.

  JCI Insight 11 ( 18 ) e200761 2026.09

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that urgently requires effective treatment. Mitochondrial dysfunction underlies ALS pathology and represents a potential therapeutic target. Here, we demonstrated the therapeutic potential of mitochonic acid 5 (MA-5), a novel mitochondria-targeted compound that ameliorated ALS phenotypes by enhancing mitochondrial function. In a Drosophila ALS model expressing a mutant human SOD1 (G85R), MA-5 significantly improved locomotor activity, with a trend toward restoration of mitochondrial integrity. In skin fibroblasts derived from ALS patients and motor neurons derived from induced pluripotent stem cells, MA-5 restored ATP production and increased mitochondrial motility. Multiomics analyses suggested that MA-5 modulated mitochondria-linked gene expression and downregulated the glycerophosphate shuttle, contributing to mitochondrial reactive oxygen species production. Transcriptomic analysis identified C7orf31 as a potential marker for monitoring the therapeutic effects of MA-5 and diagnosing ALS subtypes. These findings support MA-5 as a promising therapeutic candidate for ALS and propose C7orf31 as a potential biomarker for treatment monitoring and for disease subtyping.

  • Access to Document (DOI)

display all >>

Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Development of metabolome profiling method and application to cancer screening

  Soga, Tomoyoshi

  Keio SFC journal (慶應SFC学会)  22 ( 2 ) 320 - 330 2022

  ISSN  13472828

• Development of risk prediction models for mild cognitive decline and frailty using metabolomics in a population-based cohort

  Soga, Tomoyoshi

  科学研究費補助金研究成果報告書 2022

• Identifying functional metabolites that decide cell fates of cancer stem cells

  Soga, Tomoyoshi

  科学研究費補助金研究成果報告書 2022

• Metabolome-wide metabolic profiling and non-communicable disease prevention in a population-based cohort study

  Soga, Tomoyoshi

  科学研究費補助金研究成果報告書 2018

• Metabolomics : a new approach to elucidating biosystems

  Soga, Tomoyoshi

  Keio SFC journal (慶應義塾大学湘南藤沢学会)  15 ( 1 ) 64 - 75 2015

  ISSN  13472828

display all >>

Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• メタボロームデータの取得

  Soga, T.

  Experimental Medicine (YODOSHA)     86 - 94 2025.03

  Article, review, commentary, editorial, etc. (other), Single Work

• タボローム測手技術の開発と生命科学における意義

  Soga, T.

  SEIKAGAKU (The Japanese Biochemical Society)  96 ( 2 ) 222 - 231 2024.04

  Article, review, commentary, editorial, etc. (other), Single Work

• マルチオミクスによる大腸がんの代謝解析 100年来のがん代謝の謎を解明

  Soga, T.

  CHEMISTRY & CHEMICAL INDUSTRY (The Chemical Society Of Japan)  77 ( 4 ) 268 - 270 2024.04

  Article, review, commentary, editorial, etc. (other), Single Work

• メタボローム解析からマルチオミクスへの展開―マルチオミクスによるがん代謝解析

  Soga, T.

  Experimental Medicine (YODOSHA)  41 ( 15 ) 59(2399) - 67(2407) 2023.09

  Article, review, commentary, editorial, etc. (other), Single Work

• 腫瘍における分岐鎖アミノ酸トランスポーターの役割

  Saito, Y., Soga, T.

  Experimental Medicine (YODOSHA)  40 ( 14 ) 2239 - 2244 2022.08

  Article, review, commentary, editorial, etc. (other), Joint Work

display all >>

Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• Development of CE-MS Metabolomics and Its Application in Cancer

  Tomoyoshi Soga

  [International presentation]  AOMS2025, The 10th Asia-Oceania Mass Spectrometry Conference (ANA InterContinental, Ishigaki island, Okinawa) , 

  2025.06

  , 

  Oral presentation (invited, special), he Mass Spectrometry Society of Japan

• Development of CE-MS Metabolomics and its Application in Cancer

  Tomoyoshi Soga

  [International presentation]  Metabolomics2024, 20th Annual Confereence of the Metabolomics Society (Osaka) , 

  2024.06

• Multi Omics analysis of colorectal cancer metabolism

  [International presentation]  2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 

  2018.10

  , 

  Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

• Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

  SOGA TOMOYOSHI

  [International presentation]  The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 

  2017.11

  , 

  Oral presentation (invited, special)

• Onco-metabolites and cancer specific metabolic pathways

  SOGA TOMOYOSHI

  [International presentation]  American Association for Cancer Research Annual Meeting 2017, AACR2017, 

  2017.04

  , 

  Oral presentation (invited, special)

display all >>

Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• Study of therapeutic approaches to SUCLA2-deleted cancers

  2024.05

  -

  2026.03

  Japan Agency for Medical Research and Development(AMED), Project for Promotion of Cancer Research and Therapeutic Evolution（P-PROMOTE）, Takahashi, Chiaki, Commissioned ｒesearch, Coinvestigator(s)

• Transomic analysis of hybernation and torpor Planned Research

  2023.06

  -

  2026.03

  Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Transformative Research Areas (A), Kuroda, Shinya, Research grant, Coinvestigator(s)

• 大腸がんにおけるオンコメタボライト・L-2HGの分子基盤の解明

  2022.06

  -

  2025.03

  Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Scientific Research (C), Tabata, Sho, Other, Coinvestigator(s)

• Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems

  2021.10

  -

  2026.03

  Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned ｒesearch, Coinvestigator(s)

• Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer

  2021.05

  -

  2023.03

  Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned ｒesearch, Coinvestigator(s)

display all >>

Works 【 Display / hide 】

Works 【 Display / hide 】

• 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

  SOGA TOMOYOSHI

  2004.12

  , 

  Other, Joint

• 日経BP社主催バイオファイナンスギルド　メタボローム講座

  SOGA TOMOYOSHI

  2004.08

  , 

  Other, Joint

• キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析

  そがともよし

  2001.10

  -

  Present

  , 

  Other

  　View Details

  キャピラリー電気泳動法の装置、測定法の原理および様々な測定例を解説した

Intellectual Property Rights, etc. 【 Display / hide 】

Intellectual Property Rights, etc. 【 Display / hide 】

• カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

  Date applied： 特願平01-272206  1989.10 

  Date announced： 特開平03-134561  1991.06 

  Date issued： 特許第2833058号  1998.10

  Patent, Single

• 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

  Date applied： 特願平08-143048  1996.06 

  Date announced： 特開平09-325130  1997.12 

  Date issued： 特許第2912232号  1999.04

  Patent, Single

• キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

  Date applied： 特願平10-145244  1998.05 

  Date announced： 特開平11-337524  1999.12 

  Date issued： 特許第3038184号  2000.02

  Patent, Single

• 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

  Date applied： 特願2001-224341  2001.07 

  Date announced： 特開2003-035698  2003.02 

  Date issued： 特許第3341765号  2002.08

  Patent, Single

• 電気泳動測定によるイオン性化合物の移動時間予想方法

  Date applied： 特願2004-245728  2004.08 

  Date announced： 特開2006-064472  2006.03 

  Date issued： 特許第3871689号  2006.10

  Patent, Joint

display all >>

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• Fukuzawa Award

  Soga, T., 2022.11, Keio Univ., メタボローム（細胞内全代謝物質）解析技術の開発と実用化

  Type of Award： Keio commendation etc.

• The 2nd Shigeru Terabe Award

  2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

  Type of Award： Award from Japanese society, conference, symposium, etc.

• Keio Award

  SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

  Type of Award： Keio commendation etc.

• 第7回酸化ストレスと肝研究会　奨励賞

  SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

  Type of Award： Award from Japanese society, conference, symposium, etc.

  　View Description

  第7回酸化ストレスと肝研究会における研究発表による

• The prize of the chairman of HATSUMEI KYOKAI

  SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

  Type of Award： Other

display all >>

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• INDEPENDENT RESEARCH

  2024

• GRADUATION PROJECT 2

  2024

• GRADUATION PROJECT 1

  2024

• ADVANCED RESEARCH(SYSTEMS BIOLOGY)

  2024

• ACADEMIC PROJECT(ADVANCED BIOSCIENCES B)

  2024

display all >>