Soga, Tomoyoshi



Graduate School of Media and Governance (Shonan Fujisawa)



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Message from the Faculty Member 【 Display / hide

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

Other Disclosed Information 【 Display / hide

  • Metabolomics, Analytical Chemistry

Career 【 Display / hide

  • 1984.04

    Application Chemist, Yokogawa Corp.

  • 1992.04

    Yokogawa Analytical Systems Inc.

  • 2001.04

    University of the Ryukyus, Visiting Professor

  • 2001.04

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07

    Human Metabolome Technologies Inc., Director

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Academic Background 【 Display / hide

  • 1980.04

    Keio University, Faculty of Engineering, Applied Chemistry

    University, Graduated

Academic Degrees 【 Display / hide

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03


Research Themes 【 Display / hide

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質(メタボローム)の測定法, 



Books 【 Display / hide

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23), Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page: 307-313

     View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page: 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page: 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page: 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page: 134-160


    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page: 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page: 113-122

     View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide

  • Serum Carbohydrate Antigen 19-9 and Metabolite Hypotaurine Are Predictive Markers for Early Recurrence of Pancreatic Ductal Adenocarcinoma

    Nagao, M., Oshima, M., Suto, H., Sugimoto, M., Enomoto, A., Murakami, T., Shimomura, A., Wada, Y., Matsukawa, H., Ando, Y., Kishino, T., Kumamoto, K., Kobara, H., Kamada, H., Masaki, T., Soga, T., Okano, K.

    Pancreas 53 ( 4 ) e301 - e309 2024.04

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Objective: A significant number of patients experience early recurrence after surgical resection for pancreatic ductal adenocarcinoma (PDAC), negating the benefit of surgery. The present study conducted clinicopathologic and metabolomic analyses to explore the factors associated with the early recurrence of PDAC.

    Materials and methods: Patients who underwent pancreatectomy for PDAC at Kagawa University Hospital between 2011 and 2020 were enrolled. Tissue samples of PDAC and nonneoplastic pancreas were collected and frozen immediately after resection. Charged metabolites were quantified by capillary electrophoresis-mass spectrometry. Patients who relapsed within 1 year were defined as the early recurrence group.

    Results: Frozen tumor tissue and nonneoplastic pancreas were collected from 79 patients. The clinicopathologic analysis identified 11 predictive factors, including preoperative carbohydrate antigen 19-9 levels. The metabolomic analysis revealed that only hypotaurine was a significant risk factor for early recurrence. A multivariate analysis, including clinical and metabolic factors, showed that carbohydrate antigen 19-9 and hypotaurine were independent risk factors for early recurrence (P = 0.045 and P = 0.049, respectively). The recurrence-free survival rate 1 year after surgery with both risk factors was only 25%.

    Conclusions: Our results suggested that tumor hypotaurine is a potential metabolite associated with early recurrence. Carbohydrate antigen 19-9 and hypotaurine showed a vital utility for predicting early recurrence.

  • Genetic mutation in Escherichia coli genome during adaptation to the murine intestine is optimized for the host diet

    Tsukimi, T., Obana, N., Shigemori, S., Arakawa, K., Miyauchi, E., Yang, J., Song, I., Ashino, Y., Wakayama, M., Soga, T., Tomita, M., Ohno, H., Mori, H., Fukuda, S.

    mSystems 9 ( 2 ) e0112323 - e0112323 2024.02

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    The gut microbiota is closely associated with human health and is greatly impacted by the host diet. Bacteria such as Escherichia coli live in the gut all throughout the life of a human host and adapt to the intestinal environment. Adaptive mutations in E. coli are reported to enhance fitness in the mammalian intestine, but to what extent is still poorly known. It is also unknown whether the host diet affects what genes are mutated and to what extent fitness is affected. This study suggests that genetic mutations in the E. coli K-12 strain are selected in response to the intestinal environment and facilitate efficient utilization of abundant nutrients in the germ-free mouse intestine. Our study provides a better understanding of these intestinal adaptation mechanisms of gut microbes.

  • Association of Nonalcoholic Fatty Liver Disease with Arterial Stiffness and its Metabolomic Profiling in Japanese Community-Dwellers

    Hirata, A., Harada, S., Iida, M., Kurihara, A., Fukai, K., Kuwabara, K., Kato, S., Matsumoto, M., Sata, M., Miyagawa, N., Toki, R., Edagawa, S., Sugiyama, D., Sato, A., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Okamura, T., Takebayashi, T.

    J. Atheroscler. Thromb. online 2024.02

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Aims: Nonalcoholic fatty liver disease (NAFLD) is known to be associated with atherosclerosis. This study focused on upstream changes in the process by which NAFLD leads to atherosclerosis. The study aimed to confirm the association between NAFLD and the cardio-ankle vascular index (CAVI), an indicator of subclinical atherosclerosis, and explore metabolites involved in both by assessing 94 plasma polar metabolites.

    Methods: A total of 928 Japanese community-dwellers (306 men and 622 women) were included in this study. The association between NAFLD and CAVI was examined using a multivariable regression model adjusted for confounders. Metabolites commonly associated with NAFLD and CAVI were investigated using linear mixed-effects models in which batch numbers of metabolite measurements were used as a random-effects variable, and false discovery rate-adjusted p-values were calculated. To determine the extent to which these metabolites mediated the association between NAFLD and CAVI, mediation analysis was conducted.

    Results: NAFLD was positively associated with CAVI (coefficients [95% Confidence intervals (CI)]=0.23 [0.09-0.37]; p=0.001). A total of 10 metabolites were involved in NAFLD and CAVI, namely, branched-chain amino acids (BCAAs; valine, leucine, and isoleucine), aromatic amino acids (AAAs; tyrosine and tryptophan), alanine, proline, glutamic acid, glycerophosphorylcholine, and 4-methyl-2-oxopentanoate. Mediation analysis showed that BCAAs mediated more than 20% of the total effect in the association between NAFLD and CAVI.

    Conclusions: NAFLD was associated with a marker of atherosclerosis, and several metabolites related to insulin resistance, including BCAAs and AAAs, could be involved in the process by which NAFLD leads to atherosclerosis.

    Keywords: Atherosclerosis; Branched-chain amino acids; Cardio-ankle vascular index; Insulin resistance; Metabolites; Nonalcoholic fatty liver disease.

  • Reliability of Time-Series Plasma Metabolome Data over 6 Years in a Large-Scale Cohort Study

    Miyake, A., Harada, S., Sugiyama, D., Matsumoto, M., Hirata, A., Miyagawa, N., Toki, R., Edagawa, S., Kuwabara, K., Okamura, T., Sato, A., Amano, K., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Arakawa, K., Takebayashi, T., Iida, M.

    Metabolites  14 ( 1 ) 77 - 77 2024.01

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Studies examining long-term longitudinal metabolomic data and their reliability in large-scale populations are limited. Therefore, we aimed to evaluate the reliability of repeated measurements of plasma metabolites in a prospective cohort setting and to explore intra-individual concentration changes at three time points over a 6-year period. The study participants included 2999 individuals (1317 men and 1682 women) from the Tsuruoka Metabolomics Cohort Study, who participated in all three surveys-at baseline, 3 years, and 6 years. In each survey, 94 plasma metabolites were quantified for each individual and quality control (QC) sample. The coefficients of variation of QC, intraclass correlation coefficients, and change rates of QC were calculated for each metabolite, and their reliability was classified into three categories: excellent, fair to good, and poor. Seventy-six percent (71/94) of metabolites were classified as fair to good or better. Of the 39 metabolites grouped as excellent, 29 (74%) in men and 26 (67%) in women showed significant intra-individual changes over 6 years. Overall, our study demonstrated a high degree of reliability for repeated metabolome measurements. Many highly reliable metabolites showed significant changes over the 6-year period, suggesting that repeated longitudinal metabolome measurements are useful for epidemiological studies.

  • Metabolic Hallmarks for Purine Nucleotide Biosynthesis in Small-Cell Lung Carcinoma

    Tabata, S., Umemura, S., Narita, M., Udagawa, H., Ishikawa, T., Tsuboi, M., Goto, K., Ishii, G., Tsuchihara, K., Ochiai, A., Kobayashi, S., Soga, T., Makinoshima, H.

    Mol. Cancer Res. 22 ( 1 ) 82 - 93 2024.01

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Small-cell lung cancer (SCLC) has a poor prognosis, emphasizing the necessity for developing new therapies. The de novo synthesis pathway of purine nucleotides, which is involved in the malignant growth of SCLC, has emerged as a novel therapeutic target. Purine nucleotides are supplied by two pathways: de novo and salvage. However, the role of the salvage pathway in SCLC and the differences in utilization and crosstalk between the two pathways remain largely unclear. Here, we found that deletion of the HPRT1 gene, which codes for the rate-limiting enzyme of the purine salvage pathway, significantly suppressed tumor growth in vivo in several SCLC cells. We also demonstrated that HPRT1 expression confers resistance to lemetrexol (LMX), an inhibitor of the purine de novo pathway. Interestingly, HPRT1-knockout had less effect on SCLC SBC-5 cells, which are more sensitive to LMX than other SCLC cell lines, suggesting that a preference for either the purine de novo or salvage pathway occurs in SCLC. Furthermore, metabolome analysis of HPRT1-knockout cells revealed increased intermediates in the pentose phosphate pathway and elevated metabolic flux in the purine de novo pathway, indicating compensated metabolism between the de novo and salvage pathways in purine nucleotide biosynthesis. These results suggest that HPRT1 has therapeutic implications in SCLC and provide fundamental insights into the regulation of purine nucleotide biosynthesis.

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • 腫瘍における分岐鎖アミノ酸トランスポーターの役割

    Saito, Y., Soga, T.

    Experimental Medicine (YODOSHA)  40 ( 14 ) 2239 - 2244 2022.08

    Article, review, commentary, editorial, etc. (other), Joint Work

  • アミノ酸トランスポーターを標的とした個別化医療

    Precision Medicine 4 ( 13 ) 18 - 22 2021.11

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボロミクスによるがん幹細胞の代謝研究


    (別冊 医学のあゆみ) 治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    (別冊・医学のあゆみ) メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

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Presentations 【 Display / hide

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 


    Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism


    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 


    Oral presentation (invited, special)

  • Onco-metabolites and cancer specific metabolic pathways


    American Association for Cancer Research Annual Meeting 2017, AACR2017, 


    Oral presentation (invited, special)

  • What Causes Altered Metabolism in Colon Cancer Cells


    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 


    Oral presentation (invited, special)

  • CE-MS Metabolomics and Application to Cancer cell Metabolism


    11th International Conference of the Metabolomics Society, Metabolomics 2015, (Hyatt Regency San Francisco Airport, Burlingame, California, USA) , 


    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems


    Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned research, Coinvestigator(s)

  • Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer


    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned research, Coinvestigator(s)

  • Mitochondrial Medicine


    Japan Agency for Medical Research and Development(AMED), Moonshot Research and Development Program, Abe, Takaaki, Commissioned research, Coinvestigator(s)

  • Drug development targeting metabolic vulnerability of SUCLA2 deletion


    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , TAKAHASHI, Chiaki, Commissioned research, Coinvestigator(s)

  • Development of risk prediction models for mild cognitive decline and frailty using metabolomics in a population-based cohort


    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Takebayashi, Toru, Research grant, Coinvestigator(s)

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Works 【 Display / hide

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師



    Other, Joint

  • 日経BP社主催バイオファイナンスギルド メタボローム講座



    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析




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Intellectual Property Rights, etc. 【 Display / hide

  • カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

    Date applied: 特願平01-272206  1989.10 

    Date announced: 特開平03-134561  1991.06 

    Date issued: 特許第2833058号  1998.10

    Patent, Single

  • 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

    Date applied: 特願平08-143048  1996.06 

    Date announced: 特開平09-325130  1997.12 

    Date issued: 特許第2912232号  1999.04

    Patent, Single

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

    Date applied: 特願平10-145244  1998.05 

    Date announced: 特開平11-337524  1999.12 

    Date issued: 特許第3038184号  2000.02

    Patent, Single

  • 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

    Date applied: 特願2001-224341  2001.07 

    Date announced: 特開2003-035698  2003.02 

    Date issued: 特許第3341765号  2002.08

    Patent, Single

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    Date applied: 特願2004-245728  2004.08 

    Date announced: 特開2006-064472  2006.03 

    Date issued: 特許第3871689号  2006.10

    Patent, Joint

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Awards 【 Display / hide

  • Fukuzawa Award

    Soga, T., 2022.11, Keio Univ., メタボローム(細胞内全代謝物質)解析技術の開発と実用化

    Type of Award: Keio commendation etc.

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award: Keio commendation etc.

  • 第7回酸化ストレスと肝研究会 奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award: Award from Japanese society, conference, symposium, etc.

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  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award: Other

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Courses Taught 【 Display / hide











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