KEIO RESEARCHERS INFORMATION SYSTEM

Message from the Faculty Member 【 Display / hide 】

Message from the Faculty Member 【 Display / hide 】

• 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

Other Disclosed Information 【 Display / hide 】

Other Disclosed Information 【 Display / hide 】

• Metabolomics, Analytical Chemistry

Career 【 Display / hide 】

Career 【 Display / hide 】

• 1984.04

  -

  1992.03

  Application Chemist, Yokogawa Corp.

• 1992.04

  -

  2001.03

  Yokogawa Analytical Systems Inc.

• 2001.04

  -

  2002.03

  University of the Ryukyus, Visiting Professor

• 2001.04

  -

  2005.03

  Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

• 2003.07

  -

  2010.03

  Human Metabolome Technologies Inc., Director

display all >>

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 1980.04

  -

  1984.03

  Keio University, Faculty of Engineering, Applied Chemistry

  University, Graduated

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• Ph.D., Toyohashi University of Technology, Dissertation, 2000.03

Research Themes 【 Display / hide 】

Research Themes 【 Display / hide 】

• 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質（メタボローム）の測定法, 

  　

Books 【 Display / hide 】

Books 【 Display / hide 】

• Advances in Microscale Electro-and Liquid Phase-Based Separation Techniques

  El Rassi, Z., Ed. (Soga, T., contribution for Chapter Chapter 9), Elsevier Inc., 2026.03

  Scope: Chapter 9: “Capillary electrophoresis-mass spectrometry”,  Contact page： pp.279-294

  　View Summary

  Capillary electrophoresis-mass spectrometry (CE-MS) has gained a great deal of attention as a powerful tool for the analysis of charged compounds. This approach offers high-resolution, high-sensitivity, and wide versatility. Over the past three decades, several CE-MS methods have been developed and have made significant advances in analytical chemistry. In this chapter, the general strategies and principles of CE-MS methods will be discussed, including CE separation modes, interface techniques, mass spectrometry, new techniques to increase the throughput capability of CE-MS analysis, metabolomics, and even single-cell analysis, which has received much attention in recent years. Finally, general conclusions and perspectives are provided.

  • Access to Document (DOI)

• Amino Acid Analysis : Methods and Protocols

  Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23）, Humana Press, 2019.07

  Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page： 307-313

  　View Summary

  Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

• Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

  Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page： 430

  Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page： 223-234

• Capillary Electrophoresis-Mass Spectrometry for Metabolomics

  Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page： 300

  Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page： 134-160

• ONCO-METABOLOMICS; A NEW CLUE TO UNDERSTAND CARCINOGENESIS, CANCER BIOLOGY AND TO DEVELOP NOVEL DIAGNOSTICS AND THERAPEUTICS

  Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

display all >>

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Elevated glucose metabolism via the hexosamine biosynthesis pathway: A metabolic signature of high-fluorodeoxyglucose-uptake lung adenocarcinoma

  Watanabe, H., Makinoshima, H., Kanauchi, N., Kabasawa, T., Suzuki, J., Takamori, S., Sasage, T., Abe, K., Hoshijima, K., Uchida, T., Soga, T., Shiono, S.

  Surg. Today  2026.03

  Joint Work, Accepted

  　View Summary

  Purpose
  To evaluate hexosamine biosynthetic pathway (HBP) involvement in high-fluorodeoxyglucose (FDG)-uptake lung adenocarcinoma.
  
  Methods
  We conducted metabolomic analysis to evaluate the HBP in patients with lung adenocarcinoma, who underwent preoperative 18-FDG positron emission tomography. Capillary electrophoresis-time-of-flight mass spectrometry was done to obtain 511 small-molecule metabolite spectra, and a principal component analysis was performed.
  
  Results
  We examined 80 tissue samples: 40 tumor-adjacent non-tumor tissue samples and 40 resected lung adenocarcinomas. The principal component analysis confirmed good clustering between the tumor and non-tumor tissues. The non-tumor tissues comprised uniform materials, whereas the tumor tissues comprised a mixture of materials. Heatmaps for 50 metabolites revealed lower glucose and citrate levels and higher levels of lactate, glycolysis metabolites, succinate, fumarate, adenosine di- and -monophosphate, and all essential amino acids in the tumor tissues than the non-tumor tissues. HBP intermediate and uridine diphosphate N-acetylglucosamine levels were also higher in the tumor tissues. Both lactate and HBP intermediate levels were higher in hypermetabolic tumor tissues (standardized uptake value ≥ 3) than in non-hypermetabolic tumor tissues (standardized uptake value < 3). Low-FDG-uptake cells showed strong expression for glucose transporter SLC2A1 and weak expression for O-linked N-acetylglucosamine, whereas high-FDG-uptake cells showed strong expression for both markers.
  
  Conclusions
  Hypermetabolic adenocarcinoma may be associated with intensified glycolysis and HBP activation.

  • Access to Document (DOI)

• Global loss of metabolic responsiveness and elevated enzyme in leptin deficient obese mice during starvation

  Li, D., Morita, K., Kokaji, T., Hatano, A., Hirayama, A., Soga, T., Suzuki, Y., Matsumoto, M., Tsuchiya, T., Ozaki, H., Ohno, S., Inoue, H., Inaba, Y., Sugimoto, H., Pan, Y., Kuroda, S.

  npj Syst. Biol. Appl. online 2026.03

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  Starvation induces complex metabolic adaptations in skeletal muscle, a key tissue for maintaining energy homeostasis; however, these adaptations are largely impaired in obesity. How obesity alters global metabolic adaptations to starvation in skeletal muscle remains unclear. Here, we analyzed the metabolic adaptations on a trans-omics scale during starvation in skeletal muscle from wild-type (WT) and leptin-deficient obese (ob/ob) mice. We measured multi-omics data during starvation and constructed global trans-omics networks in WT and ob/ob mice. We found that starvation induces “responsiveness” in WT mice, characterized by increases or decreases in key regulator metabolites, including ATP and AMP, as well as enzyme proteins, leading to global regulation of metabolic pathways, which was lost in ob/ob mice. In contrast, during starvation, ob/ob mice exhibit “difference” in comparison to WT mice, manifested by the persistently elevated expression of metabolic enzymes. These features were similarly found in liver, another key metabolic organ. Thus, global loss of responsiveness and elevated enzyme proteins are systemic features of metabolic dysregulation in ob/ob mice.

  • Access to Document (DOI)

• Guanine nucleotides drive ribosome biogenesis and glycolytic reprogramming in acute myeloid leukemia stem cells

  Kawano, G., Ikeda, R., Ishihara, D., Shima, T., Sakoda, T., Yamamoto, S., Kochi, Y., Semba, Y., Ashitani, S., Mori, Y., Kato K., Maeda, T., Miyamoto, T., Soga, T., Akashi, K., Kikushige, Y.

  Blood 147 ( 7 ) 768 - 782 2026.02

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  Therapy resistance in acute myeloid leukemia (AML) remains a major clinical obstacle, particularly due to the persistence of leukemia stem cells (LSCs) capable of metabolic adaptation. While venetoclax (Ven) inhibits oxidative phosphorylation (OXPHOS), we found that Ven-resistant LSCs undergo glycolytic reprogramming to bypass OXPHOS inhibition. This metabolic shift is supported by enhanced ribosome biogenesis, sustained by upregulated de novo guanine nucleotide biosynthesis. Abundant guanine nucleotides suppress the impaired ribosome biogenesis checkpoint (IRBC), leading to TP53 destabilization and persistent MYC expression. Inhibition of inosine monophosphate dehydrogenases (IMPDH1/2) depletes guanine nucleotides, activates IRBC, stabilizes TP53, represses MYC, and impairs the metabolic shift to glycolysis. This metabolic rewiring disrupts LSC stemness and suppresses the reconstitution of human AML cells in xenotransplantation experiments. Notably, the suppression of LSC stemness was observed regardless of Ven resistance or the TP53 mutational status of AML cells. These findings reveal that mutation-independent TP53 inactivation is involved in resistant AML and suggest that targeting guanine nucleotide biosynthesis may offer a clinically actionable strategy to eradicate therapy-resistant LSCs.

  • Access to Document (DOI)

• Cross-sectional associations between CES-D scores and the salivary metabolome in nonclinical adults

  Kubo, Y., Yamauchi, F., Goshima, N., Ohishi, M., Hasegawa, T., Soga, T., Ikeda, M., Aoki, K.

  Discov. Ment. Heatlh 6 ( 1 ) 15 - 15 2026.01

  Research paper (scientific journal), Joint Work, Accepted

  　View Summary

  Background: Depression represents a major global disease burden. While the Center for Epidemiologic Studies Depression Scale (CES-D) is widely used, it relies on subjective self-reporting. Saliva enables non-invasive metabolite analysis, but existing metabolomics studies have focused on clinical depression using blood or urine, with limited investigation of salivary metabolites in community populations. This study investigated associations between CES-D scores and salivary metabolite concentrations in Tokyo residents.
  
  Methods: This cross-sectional study collected CES-D responses and morning saliva samples from Tokyo residents. Salivary metabolites were analyzed using capillary electrophoresis time-of-flight mass spectrometry. Statistical analyses included permutational multivariate analysis of variance (PERMANOVA) for overall metabolite-CES-D associations, pathway enrichment analysis using fast gene set enrichment analysis (FGSEA), and principal component analysis, adjusted for age and sex.
  
  Results: Analysis included 107 participants (40 males, 67 females; mean age 55.3 ± 14.2 years). PERMANOVA revealed significant association between 112 salivary metabolites and CES-D scores (F = 2.870, p = 0.003), with metabolites explaining 7.7% of depressive symptom variance. Pathway enrichment analysis identified significant alterations in amino acid metabolism (normalized enrichment score =-1.750, q = 0.007). Glycolysis and amine/polyamine metabolism showed trend-level enrichment (both q = 0.066). Principal component analysis revealed significant correlations between CES-D scores and pathway activity for amino acid metabolism (r = 0.216, p = 0.027) and amine/polyamine metabolism (r = 0.216, p = 0.027).
  
  Conclusion: This study demonstrates significant associations between salivary metabolite profiles and depressive symptoms in a nonclinical community population, with amino acid metabolism emerging as the most robustly altered pathway. These findings provide preliminary evidence for associations between salivary metabolomic profiles and depressive symptoms in a nonclinical community population.

  • Access to Document (DOI)

• Abrogation of aberrant glycolytic interactions eliminates senescent cells and alleviates aging-related dysfunctions

  Mikawa, T., Kameda, M., Ikari, S., Shibata, E., Liu, S., Miyagawa, S., Ono, K., Ito, T., Yoshizawa, A., Sugimoto, M., Shibuya, S., Shimizu, T., Almunia, J., Ogiso, N., Revêchon, G., Palazzo, A., Bernard, D., Kanda, H., Soga, T., Takubo, K., Morioka, S., Sasaki, J., Sasaki, T., Itamoto, A., Fujii, T., Seno, H., Inagaki, N., Kondoh, H.

  Signal Transduct. Target. Ther.  10 ( 1 ) 402 - 402 2025.12

  Research paper (scientific journal), Joint Work, Accepted,  ISSN  20959907

  　View Summary

  Cellular senescence is deeply involved in physiological homeostasis, development, tissue repair, aging, and diseases. Senescent cells (SnCs) accumulate in aged tissues and exert deleterious effects by secreting proinflammatory molecules that contribute to chronic inflammation and aging-related diseases. We revealed that an aberrant interaction between glycolytic PGAM1 and Chk1 kinase is augmented in SnCs associated with increased glycolysis, whose byproduct, lactate, promotes this binding in a noncell autonomous manner. The pseudo-Warburg effect of SnCs with enhanced PPP (pentose phosphate pathway) activity is maintained by HIF-2α phosphorylation by Chk1 and subsequent upregulation of glycolytic enzymes, creating a vicious cycle reprogramming the glycolytic pathway in SnCs. HIF-2α also activates FoxM1 expression, which transcriptionally suppresses proapoptotic profiles, including BIM, and upregulates DNA repair machineries in SnCs. FoxM1 thus supports the genomic integrity and survival capacity of SnCs during their glycolytic changes. Chemical abrogation of PGAM1-Chk1 binding reverts these phenotypes and eliminates SnCs through senolysis. Inhibition of the PGAM1-Chk1 interaction improves physiological parameters during aging and inhibits lung fibrosis in mouse models. Our study highlights a novel pathway contributing to the metabolic reprogramming of SnCs and how the use of a new senolytic molecule that targets the PGAM-Chk1 interaction creates a specific vulnerability of those cells to potentially fight age-related diseases.

  • Access to Document (DOI)

display all >>

Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Development of risk prediction models for mild cognitive decline and frailty using metabolomics in a population-based cohort

  Soga, Tomoyoshi

  科学研究費補助金研究成果報告書 2022

• Identifying functional metabolites that decide cell fates of cancer stem cells

  Soga, Tomoyoshi

  科学研究費補助金研究成果報告書 2022

• Development of metabolome profiling method and application to cancer screening

  Soga, Tomoyoshi

  Keio SFC journal (慶應SFC学会)  22 ( 2 ) 320 - 330 2022

  ISSN  13472828

• Metabolome-wide metabolic profiling and non-communicable disease prevention in a population-based cohort study

  Soga, Tomoyoshi

  科学研究費補助金研究成果報告書 2018

• Comprehensive analysis of microbes and metabolites in human tear fluids

  Soga, Tomoyoshi

  Keio SFC journal (慶應義塾大学湘南藤沢学会)  15 ( 1 ) 382 - 400 2015

  ISSN  13472828

display all >>

Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• メタボロームデータの取得

  Soga, T.

  Experimental Medicine (YODOSHA)     86 - 94 2025.03

  Article, review, commentary, editorial, etc. (other), Single Work

• タボローム測手技術の開発と生命科学における意義

  Soga, T.

  SEIKAGAKU (The Japanese Biochemical Society)  96 ( 2 ) 222 - 231 2024.04

  Article, review, commentary, editorial, etc. (other), Single Work

• マルチオミクスによる大腸がんの代謝解析 100年来のがん代謝の謎を解明

  Soga, T.

  CHEMISTRY & CHEMICAL INDUSTRY (The Chemical Society Of Japan)  77 ( 4 ) 268 - 270 2024.04

  Article, review, commentary, editorial, etc. (other), Single Work

• メタボローム解析からマルチオミクスへの展開―マルチオミクスによるがん代謝解析

  Soga, T.

  Experimental Medicine (YODOSHA)  41 ( 15 ) 59(2399) - 67(2407) 2023.09

  Article, review, commentary, editorial, etc. (other), Single Work

• 腫瘍における分岐鎖アミノ酸トランスポーターの役割

  Saito, Y., Soga, T.

  Experimental Medicine (YODOSHA)  40 ( 14 ) 2239 - 2244 2022.08

  Article, review, commentary, editorial, etc. (other), Joint Work

display all >>

Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• Development of CE-MS Metabolomics and Its Application in Cancer

  Tomoyoshi Soga

  [International presentation]  AOMS2025, The 10th Asia-Oceania Mass Spectrometry Conference (ANA InterContinental, Ishigaki island, Okinawa) , 

  2025.06

  , 

  Oral presentation (invited, special), he Mass Spectrometry Society of Japan

• Development of CE-MS Metabolomics and its Application in Cancer

  Tomoyoshi Soga

  [International presentation]  Metabolomics2024, 20th Annual Confereence of the Metabolomics Society (Osaka) , 

  2024.06

• Multi Omics analysis of colorectal cancer metabolism

  [International presentation]  2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 

  2018.10

  , 

  Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

• Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

  SOGA TOMOYOSHI

  [International presentation]  The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 

  2017.11

  , 

  Oral presentation (invited, special)

• Onco-metabolites and cancer specific metabolic pathways

  SOGA TOMOYOSHI

  [International presentation]  American Association for Cancer Research Annual Meeting 2017, AACR2017, 

  2017.04

  , 

  Oral presentation (invited, special)

display all >>

Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• Study of therapeutic approaches to SUCLA2-deleted cancers

  2024.05

  -

  2026.03

  Japan Agency for Medical Research and Development(AMED), Project for Promotion of Cancer Research and Therapeutic Evolution（P-PROMOTE）, Takahashi, Chiaki, Commissioned ｒesearch, Coinvestigator(s)

• Transomic analysis of hybernation and torpor Planned Research

  2023.06

  -

  2026.03

  Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Transformative Research Areas (A), Kuroda, Shinya, Research grant, Coinvestigator(s)

• 大腸がんにおけるオンコメタボライト・L-2HGの分子基盤の解明

  2022.06

  -

  2025.03

  Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Scientific Research (C), Tabata, Sho, Other, Coinvestigator(s)

• Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems

  2021.10

  -

  2026.03

  Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned ｒesearch, Coinvestigator(s)

• Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer

  2021.05

  -

  2023.03

  Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned ｒesearch, Coinvestigator(s)

display all >>

Works 【 Display / hide 】

Works 【 Display / hide 】

• 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

  SOGA TOMOYOSHI

  2004.12

  , 

  Other, Joint

• 日経BP社主催バイオファイナンスギルド　メタボローム講座

  SOGA TOMOYOSHI

  2004.08

  , 

  Other, Joint

• キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析

  そがともよし

  2001.10

  -

  Present

  , 

  Other

  　View Details

  キャピラリー電気泳動法の装置、測定法の原理および様々な測定例を解説した

Intellectual Property Rights, etc. 【 Display / hide 】

Intellectual Property Rights, etc. 【 Display / hide 】

• カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

  Date applied： 特願平01-272206  1989.10 

  Date announced： 特開平03-134561  1991.06 

  Date issued： 特許第2833058号  1998.10

  Patent, Single

• 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

  Date applied： 特願平08-143048  1996.06 

  Date announced： 特開平09-325130  1997.12 

  Date issued： 特許第2912232号  1999.04

  Patent, Single

• キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

  Date applied： 特願平10-145244  1998.05 

  Date announced： 特開平11-337524  1999.12 

  Date issued： 特許第3038184号  2000.02

  Patent, Single

• 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

  Date applied： 特願2001-224341  2001.07 

  Date announced： 特開2003-035698  2003.02 

  Date issued： 特許第3341765号  2002.08

  Patent, Single

• 電気泳動測定によるイオン性化合物の移動時間予想方法

  Date applied： 特願2004-245728  2004.08 

  Date announced： 特開2006-064472  2006.03 

  Date issued： 特許第3871689号  2006.10

  Patent, Joint

display all >>

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• Fukuzawa Award

  Soga, T., 2022.11, Keio Univ., メタボローム（細胞内全代謝物質）解析技術の開発と実用化

  Type of Award： Keio commendation etc.

• The 2nd Shigeru Terabe Award

  2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

  Type of Award： Award from Japanese society, conference, symposium, etc.

• Keio Award

  SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

  Type of Award： Keio commendation etc.

• 第7回酸化ストレスと肝研究会　奨励賞

  SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

  Type of Award： Award from Japanese society, conference, symposium, etc.

  　View Description

  第7回酸化ストレスと肝研究会における研究発表による

• The prize of the chairman of HATSUMEI KYOKAI

  SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

  Type of Award： Other

display all >>

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• SEMINAR B

  2024

• METABOLOMICS

  2024

• METABOLOME ANALYSIS LABORATORY PRACTICE

  2024

• MASTER SEMINAR

  2024

• INDEPENDENT RESEARCH

  2024

display all >>