Soga, Tomoyoshi

写真a

Affiliation

Graduate School of Media and Governance (Shonan Fujisawa)

Position

Professor

Related Websites

External Links
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Message from the Faculty Member 【 Display / hide 】

  • 自分のこれまでの経験では、実験がうまくいってもそこから得られるものは何もありません。失敗して、原因をあれこれ考えることで自分が知らなかった知見を得たり、新しい発見をしたりします。したがって、多くのことに果敢にチェレンジしてたくさんの失敗を重ねて欲しいと思います。失敗が必ず皆さんの糧になります。

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Other Disclosed Information 【 Display / hide 】

  • Metabolomics, Analytical Chemistry

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Career 【 Display / hide 】

  • 1984.04
    -
    1992.03

    Application Chemist, Yokogawa Corp.

  • 1992.04
    -
    2001.03

    Yokogawa Analytical Systems Inc.

  • 2001.04
    -
    2002.03

    University of the Ryukyus, Visiting Professor

  • 2001.04
    -
    2005.03

    Faculty of Environmental Information/ Institute for Advanced Biosciences, Associate Professor

  • 2003.07
    -
    2010.03

    Human Metabolome Technologies Inc., Director

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Academic Background 【 Display / hide 】

  • 1980.04
    -
    1984.03

    Keio University, Faculty of Engineering, Applied Chemistry

    University, Graduated

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Academic Degrees 【 Display / hide 】

  • Ph.D., Toyohashi University of Technology, Dissertation, 2000.03

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Research Themes 【 Display / hide 】

  • 枯草菌、大腸菌、酵母等のバクテリアからイネやマウスの組織、ヒトの血液、尿、赤血球、ガン細胞等のあらゆる生物種の細胞内全代謝物質（メタボローム）の測定法, 

    　

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Books 【 Display / hide 】

  • Amino Acid Analysis : Methods and Protocols

    Alterman M.Ed., (Hirayama, A., Ikeda, S., Sato, A., Soga, T., contribution for Chapter 23）, Humana Press, 2019.07

    Scope: Chapter 23: Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry,  Contact page： 307-313

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

  • Oceanography Challenges to Future Earth : Human and Natural Impacts on our Seas

    Komatsu, T., Ceccaldi, H-J., Yoshida, J., Prouzet, P., Henocque, Y. Ed., (Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T., contribution for Part IV ), Springer, 2019.02,  Page： 430

    Scope: Part IV Innovative Research: Metabolome profiling of growth hormone transgenic coho salmon by capillary electrophoresis time-of-flight mass spectrometry,  Contact page： 223-234

  • Capillary Electrophoresis-Mass Spectrometry for Metabolomics

    Ramautar R. Ed., (Hirayama A, Soga T. contribution for CHAPTER 7), The Royal Society of Chemistry, 2018.07,  Page： 300

    Scope: CHAPTER 7: CE-MS for anionic and cationic metabolic profiling: system optimization and applications,  Contact page： 134-160

  • ONCO-METABOLOMICS; A NEW CLUE TO UNDERSTAND CARCINOGENESIS, CANCER BIOLOGY AND TO DEVELOP NOVEL DIAGNOSTICS AND THERAPEUTICS

    Esmi, H., Mak, T.W., Soga, T., Suematsu, M.,Mori, M. Ed, Princess Takamatsu Cancer Research Fund, 2016.04

  • Metabolomics: Methods and Protocols

    Bjerrum, J. T. Ed. (Wakayama, M., Hirayama, A., Soga, T., contribution for Chapter 13), Humana Press, 2015.04,  Page： 269

    Scope: Chapter 13: Capillary Electrophoresis-Mass Spectrometry,  Contact page： 113-122

    　View Summary

    Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

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Papers 【 Display / hide 】

  • Time and dose selective glucose metabolism for glucose homeostasis and energy conversion in the liver

    Pan, Y., Hatano, A., Ohno, S., Morita, K., Kokaji, T., Bai, Y., Sugimoto, H., Egami, R., Terakawa, A., Li, D., Uematsu, S., Maehara, H., Fujita, S., Inoue, H., Inaba, Y., Nagano, A., Hirayama, A., Soga, T., Kuroda, S.

    npj Syst. Biol. Appl. 10 ( 1 ) 107 - 107 2024.09

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Hepatic glucose metabolism serves dual purposes: maintaining glucose homeostasis and converting glucose into energy sources; however, the underlying mechanisms are unclear. We quantitatively measured liver metabolites, gene expression, and phosphorylated insulin signaling molecules in mice orally administered varying doses of glucose, and constructed a transomic network. Rapid phosphorylation of insulin signaling molecules in response to glucose intake was observed, in contrast to the more gradual changes in gene expression. Glycolytic and gluconeogenic metabolites and expression of genes involved in glucose metabolism including glucose-6-phosphate, G6pc, and Pck1, demonstrated high glucose dose sensitivity. Whereas, glucokinase expression and glycogen accumulation showed low glucose dose sensitivity. During the early phase after glucose intake, metabolic flux was geared towards glucose homeostasis regardless of the glucose dose but shifted towards energy conversion during the late phase at higher glucose doses. Our research provides a comprehensive view of time- and dose-dependent selective glucose metabolism.

  • Metabolomics Profiles Alterations in Cigarette Smokers and Heated Tobacco Product Users

    Harada, S., Ohmomo, H., Matsumoto, M., Sata, M., Iida, M., Hirata, A., Miyagawa, N., Kuwabara, K., Kato, S., Toki, R., Edagawa, S., Sugiyama, D., Sato, A., Hirayama, A., Sugimoto, M., Soga, T., Tomita, M., Shimizu, A., Okamura, T., Takebayashi, T.

    J. Epidemiol. 34 ( 9 ) 403 - 410 2024.09

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Background: Heated tobacco products (HTPs) have gained global popularity, but their health risks remain unclear. Therefore, the current study aimed to identify plasma metabolites associated with smoking and HTP use in a large Japanese population to improve health risk assessment.

    Methods: Metabolomics data from 9,922 baseline participants of the Tsuruoka Metabolomics Cohort Study (TMCS) were analyzed to determine the association between smoking habits and plasma metabolites. Moreover, alterations in smoking-related metabolites among HTP users were examined based on data obtained from 3,334 participants involved from April 2018 to June 2019 in a follow-up survey.

    Results: Our study revealed that cigarette smokers had metabolomics profiles distinct from never smokers, with 22 polar metabolites identified as candidate biomarkers for smoking. These biomarker profiles of HTP users were closer to those of cigarette smokers than those of never smokers. The concentration of glutamate was higher in cigarette smokers, and biomarkers involved in glutamate metabolism were also associated with cigarette smoking and HTP use. Network pathway analysis showed that smoking was associated with the glutamate pathway, which could lead to endothelial dysfunction and atherosclerosis of the vessels.

    Conclusions: Our study showed that the glutamate pathway is affected by habitual smoking. These changes in the glutamate pathway may partly explain the mechanism by which cigarette smoking causes cardiovascular disease. HTP use was also associated with glutamate metabolism, indicating that HTP use may contribute to the development of cardiovascular disease through mechanisms similar to those in cigarette use.

    Keywords: electronic nicotine delivery devices; heated tobacco products; metabolomics; prevention; smoking-induced disease.

  • The role of cytidine 5'-triphosphate synthetase 1 in metabolic rewiring during epithelial-to-mesenchymal transition in non-small-cell lung cancer

    Nakasuka, F., Hirayama, A., Mkinoshima, H., Yano, S., Soga, T., Tabata, S.

    FEBS Open Bio 14 ( 9 ) 1570 - 1583 2024.09

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Epithelial-to-mesenchymal transition (EMT) contributes to the poor prognosis of patients with cancer by promoting distant metastasis and anti-cancer drug resistance. Several distinct metabolic alterations have been identified as key EMT phenotypes. In the present study, we further characterize the role of transforming growth factor-β (TGF-β)-induced EMT in non-small-cell lung cancer. Our study revealed that TGF-β plays a role in EMT functions by upregulation of cytidine 5'-triphosphate synthetase 1 (CTPS), a vital enzyme for CTP biosynthesis in the pyrimidine metabolic pathway. Both knockdown and enzymatic inhibition of CTPS reduced TGF-β-induced changes in EMT marker expression, chemoresistance and migration in vitro. Moreover, CTPS knockdown counteracted the TGF-β-mediated downregulation of UDP-glucuronate, glutarate, creatine, taurine and nicotinamide. These findings indicate that CTPS plays a multifaceted role in EMT metabolism, which is crucial for the malignant transformation of cancer through EMT, and underline its potential as a promising therapeutic target for preventing drug resistance and metastasis in non-small-cell lung cancer.

  • Gut microbial factors predict disease severity in a mouse model of multiple sclerosis

    Steimle, A., Neumann, M., Grant, E.T., Willieme, S., Sciscio, A.D., Parrish, A., Ollert, M., Miyauchi, E., Soga, T., Fukuda, S., Ohno, H., Desai, M.S.

    Nat. Microbiol. 9 ( 9 ) 2244 - 2261 2024.09

    Research paper (scientific journal), Joint Work, Accepted

    　View Summary

    Gut bacteria are linked to neurodegenerative diseases but the risk factors beyond microbiota composition are limited. Here we used a pre-clinical model of multiple sclerosis (MS), experimental autoimmune encephalomyelitis (EAE), to identify microbial risk factors. Mice with different genotypes and complex microbiotas or six combinations of a synthetic human microbiota were analysed, resulting in varying probabilities of severe neuroinflammation. However, the presence or relative abundances of suspected microbial risk factors failed to predict disease severity. Akkermansia muciniphila, often associated with MS, exhibited variable associations with EAE severity depending on the background microbiota. Significant inter-individual disease course variations were observed among mice harbouring the same microbiota. Evaluation of microbial functional characteristics and host immune responses demonstrated that the immunoglobulin A coating index of certain bacteria before disease onset is a robust individualized predictor of disease development. Our study highlights the need to consider microbial community networks and host-specific bidirectional interactions when aiming to predict severity of neuroinflammation.

  • FDX2, an iron-sulfur cluster assembly factor, is essential to prevent cellular senescence, apoptosis or ferroptosis of ovarian cancer cells

    Miyahara, S., Ohuchi, M., Nomura, M., Hashimoto, E., Soga, T., Saito, R., Hayashi, K., Sato, T., Saito, M., Yamashita, Y., Shimada, M., Yaegashi, N., Yamada, H., Tanuma, N.

    J. Biol. Chem. 300 ( 9 ) 107678 - 107678 2024.09

    Research paper (scientific journal), Accepted

    　View Summary

    Recent studies reveal that biosynthesis of iron-sulfur clusters (Fe-Ss) is essential for cell proliferation, including that of cancer cells. Nonetheless, it remains unclear how Fe-S biosynthesis functions in cell proliferation/survival. Here, we report that proper Fe-S biosynthesis is essential to prevent cellular senescence, apoptosis, or ferroptosis, depending on cell context. To assess these outcomes in cancer, we developed an ovarian cancer line with conditional KO of FDX2, a component of the core Fe-S assembly complex. FDX2 loss induced global downregulation of Fe-S-containing proteins and Fe2+ overload, resulting in DNA damage and p53 pathway activation, and driving the senescence program. p53 deficiency augmented DNA damage responses upon FDX2 loss, resulting in apoptosis rather than senescence. FDX2 loss also sensitized cells to ferroptosis, as evidenced by compromised redox homeostasis of membrane phospholipids. Our results suggest that p53 status and phospholipid homeostatic activity are critical determinants of diverse biological outcomes of Fe-S deficiency in cancer cells.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 腫瘍における分岐鎖アミノ酸トランスポーターの役割

    Saito, Y., Soga, T.

    Experimental Medicine (YODOSHA)  40 ( 14 ) 2239 - 2244 2022.08

    Article, review, commentary, editorial, etc. (other), Joint Work

  • アミノ酸トランスポーターを標的とした個別化医療

    Precision Medicine 4 ( 13 ) 18 - 22 2021.11

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボロミクスによるがん幹細胞の代謝研究

    北島正二朗、曽我朋義

    （別冊 医学のあゆみ）　治療標的としてのがん幹細胞 (Ishiyaku Pub,Inc.)     117 - 121 2021.03

    Article, review, commentary, editorial, etc. (other), Joint Work

  • メタボローム解析に基づく癌診断法の開発

    Soga, T., Sugimoto, M.

    Gastroenterology & Hepatology (Kagakuhyoronsya Co., Ltd.)  8 ( 2 ) 137 - 143 2020.08

    Joint Work

  • はじめに-メタボローム解析UPDATE

    Soga, T.

    （別冊・医学のあゆみ） メタボローム解析UPDATE (Ishiyaku Pub,Inc.)   2020.06

    Single Work

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Presentations 【 Display / hide 】

  • Development of CE-MS Metabolomics and its Application in Cancer

    Tomoyoshi Soga

    Metabolomics2024, 20th Annual Confereence of the Metabolomics Society (Osaka) , 

    2024.06

  • Multi Omics analysis of colorectal cancer metabolism

    2018 International Meeting on 22nd MDO and 33rd JSSX (Ishikawa Ongakudo, Kanazawa, Ishikawa) , 

    2018.10
    , 

    Oral presentation (invited, special), JSSX (The Japanese Society for the Study of Xenobiotics)、MDO(Microsomes and Drug Oxidations)

  • Malti-omics reveals MYC as a master regulator of colorectal cancer metabolism

    SOGA TOMOYOSHI

    The 1st International Symposium for Trans-Omics (Koshiba Hall, The University of Tokyo, Hongo Campus) , 

    2017.11
    , 

    Oral presentation (invited, special)

  • Onco-metabolites and cancer specific metabolic pathways

    SOGA TOMOYOSHI

    American Association for Cancer Research Annual Meeting 2017, AACR2017, 

    2017.04
    , 

    Oral presentation (invited, special)

  • What Causes Altered Metabolism in Colon Cancer Cells

    SOGA TOMOYOSHI

    46th International Symposium of The Princess Takamatsu Cancer Research Found (Palece Hotel Tokyo, Japan) , 

    2015.11
    , 

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Study of therapeutic approaches to SUCLA2-deleted cancers

    2024.05
    -
    2025.03

    Japan Agency for Medical Research and Development(AMED), Project for Promotion of Cancer Research and Therapeutic Evolution（P-PROMOTE）, Takahashi, Chiaki, Commissioned ｒesearch, Coinvestigator(s)

  • Transomic analysis of hybernation and torpor Planned Research

    2023.06
    -
    2025.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Transformative Research Areas (A), Kuroda, Shinya, Research grant, Coinvestigator(s)

  • 大腸がんにおけるオンコメタボライト・L-2HGの分子基盤の解明

    2022.06
    -
    2025.03

    Ministry of Education,Culture,Sports,Science and Technology(MEXT)/Japan Society for the Promotion of Science(JSPS) , Grant-in-Aid for Scientific Research (C), Tabata, Sho, Other, Coinvestigator(s)

  • Spatio-temporal trans-omics analysis of metabolic control mechanisms of multi-cellular organ systems

    2021.10
    -
    2025.03

    Japan Science and Technology Agency(JST), Strategic Basic Research Programs CREST, Kuroda, Shinya, Commissioned ｒesearch, Coinvestigator(s)

  • Development of novel therapy targeting SUCLA2 deficiency in advanced prostate cancer

    2021.05
    -
    2023.03

    Japan Agency for Medical Research and Development(AMED), Project for Cancer Research and Therapeutic Evolution (P-CREATE) , Takahashi, C., Commissioned ｒesearch, Coinvestigator(s)

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Works 【 Display / hide 】

  • 経済産業省主催「バイオ人材育成事業」メタボローム実習講師

    SOGA TOMOYOSHI

    2004.12
    , 

    Other, Joint

  • 日経BP社主催バイオファイナンスギルド　メタボローム講座

    SOGA TOMOYOSHI

    2004.08
    , 

    Other, Joint

  • キャピラリー電気泳動による無機陰イオン、有機酸、アミノ酸の分析

    そがともよし

    2001.10
    -
    Present
    , 

    Other

    　View Details

    キャピラリー電気泳動法の装置、測定法の原理および様々な測定例を解説した

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Intellectual Property Rights, etc. 【 Display / hide 】

  • カテコールアミン類の分析方法(Apparatus and Method for Catecholamine Analysis)

    Date applied： 特願平01-272206  1989.10 

    Date announced： 特開平03-134561  1991.06 

    Date issued： 特許第2833058号  1998.10

    Patent, Single

  • 陰イオン性化合物の分析方法(Apparatus and Method for Anion Analysis)

    Date applied： 特願平08-143048  1996.06 

    Date announced： 特開平09-325130  1997.12 

    Date issued： 特許第2912232号  1999.04

    Patent, Single

  • キャピラリー電気泳動による陰イオン、アミノ酸、糖類の分析方法及び装置(Capillary Electrophoresis Apparatus and Method for Anions, Amino Acids and Carbohydrate Analysis)

    Date applied： 特願平10-145244  1998.05 

    Date announced： 特開平11-337524  1999.12 

    Date issued： 特許第3038184号  2000.02

    Patent, Single

  • 陰イオン性化合物の分離分析方法及び装置(Apparatus and Method for Anion Analysis)

    Date applied： 特願2001-224341  2001.07 

    Date announced： 特開2003-035698  2003.02 

    Date issued： 特許第3341765号  2002.08

    Patent, Single

  • 電気泳動測定によるイオン性化合物の移動時間予想方法

    Date applied： 特願2004-245728  2004.08 

    Date announced： 特開2006-064472  2006.03 

    Date issued： 特許第3871689号  2006.10

    Patent, Joint

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Awards 【 Display / hide 】

  • Fukuzawa Award

    Soga, T., 2022.11, Keio Univ., メタボローム（細胞内全代謝物質）解析技術の開発と実用化

    Type of Award： Keio commendation etc.

  • The 2nd Shigeru Terabe Award

    2015.11,  Division of Electrophoresis, Japan Society for Analytical Chemistry, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • Keio Award

    SOGA Tomoyoshi, 2011.11, Keio University, CE-MSメタボローム測定技術の開発と実用化

    Type of Award： Keio commendation etc.

  • 第7回酸化ストレスと肝研究会　奨励賞

    SOGA Tomoyoshi, 2010.11, 酸化ストレスと肝研究会, メタボロミクスによる新規酸化ストレスマーカーの同定と肝臓疾患スクリーニング

    Type of Award： Award from Japanese society, conference, symposium, etc.

    　View Description

    第7回酸化ストレスと肝研究会における研究発表による

  • The prize of the chairman of HATSUMEI KYOKAI

    SOGA Tomoyoshi, 2009.07, Japan Institute of Invention and Innovation, Apparatus and Method for Metabolome Analysis

    Type of Award： Other

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Courses Taught 【 Display / hide 】

  • SEMINAR B

    2024

  • METABOLOMICS

    2024

  • METABOLOME ANALYSIS LABORATORY PRACTICE

    2024

  • MASTER SEMINAR

    2024

  • INDEPENDENT RESEARCH

    2024

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