Masuda, Takeshi

写真a

Affiliation

Graduate School of Media and Governance (Shonan Fujisawa)

Position

Project Senior Assistant Professor (Non-tenured)/Project Assistant Professor (Non-tenured)/Project Lecturer (Non-tenured)

Career 【 Display / hide

  • 2004.04
    -
    2012.03

    慶應義塾大学 政策・メディア研究科 特別研究員

  • 2012.04
    -
    Present

    慶應義塾大学 政策・メディア研究科 特任助教

Academic Background 【 Display / hide

  • 2004.03

    Tokyo University of Agriculture, -, 生物産業学

    Graduate School, Completed, Master's course

Academic Degrees 【 Display / hide

  • 生物産業学, Tokyo University of Agriculture, Dissertation, 2012.02

 

Papers 【 Display / hide

  • Microscale phosphoproteome analysis of 10000 cells from human cancer cell lines

    Masuda Takeshi, Sugiyama Naoyuki, Tomita Masaru, Ishihama Yasushi

    Analytical Chemistry 83 ( 20 ) 7698-7703 2011.10

    Research paper (scientific journal), Joint Work, Accepted

  • Nematode-specific tRNAs that decode an alternative genetic code for leucine

    Kiyofumi Hamashima, Kousuke Fujishima, Takeshi Masuda, Junichi Sugahara, Masaru Tomita, Akio Kanai

    Nucleic Acids Research  2011

    Research paper (scientific journal), Joint Work, Accepted

  • Unbiased Quantitation of Escherichia coli Membrane Proteome Using Phase‐Transfer Surfactants

    Takeshi Masuda, Natsumi Saito, Masaru Tomita, Yasushi Ishihama

    Molecular and Cellular Proteomics 8 ( 12 ) 2770-2777 2009.12

    Research paper (scientific journal), Single Work, Accepted

  • Chemical Cleavage-Assisted Tryptic Digestion for Membrane Proteome Analysis

    Mio Iwasaki, Takeshi Masuda, Masaru Tomita and Yasushi Ishihama

    Journal of Proteome Resarch 8 ( 6 ) 3169-3175 2009.06

    Research paper (scientific journal), Joint Work, Accepted

  • Phase Transfer Surfactant-aided Trypsin Digestion for Membrane Proteome Analysis

    Takeshi Masuda, Masaru Tomita, Yasushi Ishihama

    Journal of Proteome Resarch 7 ( 2 ) 731-740 2008.01

    Research paper (scientific journal), Single Work, Accepted

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Presentations 【 Display / hide

  • 高感度LC-MS/MSシステムを用いた極微量組織試料のプロテオーム解析技術の確立

    若林真樹,増田豪,石濱泰

    第22回クロマトグラフィー科学会議 (仙台) , 

    2011.10

    Oral presentation (general), クロマトグラフィー科学会

  • Characterization of three RNA ligases from a hyperthermophilic archaeon Pyrococcus furiosus

    Akio Kanai, Kanako Takesue, Asako Sato, Takeshi Masuda and Masaru Tomita

    第84回 日本生化学会大会 (国立京都国際会館) , 

    2011.09

    Oral presentation (general), 日本生化学会

  • 微量組織試料に対する定量的リン酸化プロテオーム解析法の確立

    若林真樹,塚原麻衣,吉原宏樹,増田豪,石濱泰

    日本プロテオーム機構第9回大会 (新潟) , 

    2011.07

    Poster presentation, 日本プロテオーム機構

  • 高感度リン酸化プロテオーム解析システムの開発

    増田豪, 五十嵐康之, 冨田勝, 並木淳, 杉山直幸, 石濱泰

    日本プロテオーム機構第9回大会 (新潟) , 

    2011.07

    Poster presentation, 日本プロテオーム機構

  • タンパク質の抽出と分画

    増田豪, 杉山直幸, 石濱泰

    日本プロテオーム機構第9回大会 (新潟) , 

    2011.07

    Oral presentation (invited, special), 日本プロテオーム機構

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Development of high-throughput single-cell proteomics system

    2022.04
    -
    2025.03

    基盤研究(B), Principal investigator

  • Development of highly sensitive quantitative proteomics for cross biology

    2022.04
    -
    2024.03

    学術変革領域研究(A), Principal investigator

  • 代謝制御機構解明のための網羅的酵素定量法

    2011.08
    -
    2012.03

    慶應義塾大学 政策・メディア研究科, 研究成果展開事業 研究成果最適展開支援プログラム (A-STEP) フィージビリティスタディ【FS】ステージ 探索タイプ, Research grant, No Setting

  • 高感度細胞表面抗原マーカー検出に向けたエピトープ領域の網羅的同定

    2011.04
    -
    2013.03

    Grant-in-Aid for Scientific Research, Research grant, No Setting

  • 国際学会等派遣事業

     

    The Science Research Promotion Fund, Research grant, No Setting

Awards 【 Display / hide

  • 2011 JHUPO Best Poster Award

    MASUDA TAKESHI, 2011.07, 日本プロテオーム学会, 高感度リン酸化プロテオーム解析システムの開発

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • BMS travel award

    MASUDA TAKESHI, 2008.01, 日本質量分析学会, Phase Transfer Surfactant-aided Trypsin Digestion for Membrane Proteome Analysis

    Type of Award: Award from Japanese society, conference, symposium, etc.

     View Description

    We have developed a new protocol for digesting hydrophobic proteins using trypsin with the aid of phase-transfer surfactants (PTS), such as sodium deoxycholate (SDC). SDC increases the solubility of hydrophobic proteins, enhances the activity of trypsin, and improves the accessibility to trypsin of proteins denatured during the extraction process. After digestion, SDC was successfully removed from the acidified solution containing tryptic peptides by adding a water-immiscible organic solvent, into which SDC was predominantly transferred, while the digested peptides remained in the aqueous phase. Compared with a protocol using an acid-labile surfactant, this PTS protocol increased the number of identified proteins and the recovery of hydrophobic peptides in the analysis of 400 ng of membrane-enriched fraction of Escherichia coli. Application of the PTS protocol to 5.4 µg of membrane-enriched pellet from human cervical cancer HeLa cells resulted in identification of a total of 1450 prot

 

Courses Taught 【 Display / hide

  • PROTEOMICS

    2024

  • PROTEOME ANALYSIS LABORATORY PRACTICE

    2024

  • PROTEOMICS

    2023

  • PROTEOME ANALYSIS LABORATORY PRACTICE

    2023