Miyamoto, Kenji

写真a

Affiliation

Faculty of Science and Technology, Department of Biosciences and Informatics （Yagami）

Position

Professor

Related Websites

External Links
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Career 【 Display / hide 】

  • 1992.10
    -
    2002.09

    鐘淵化学工業（株）生物化学研究所（現ライフサイエンス研究所） ,研究員

  • 2006.08
    -
    2007.08

    ドイツGreifswald大学（博士研究員）

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Academic Background 【 Display / hide 】

  • 1988.03

    Keio University, Faculty of Science and Engineering, 化学科

    University, Graduated

  • 1990.03

    Keio University, Graduate School, Division of Science and Engineeri, 化学専攻

    Graduate School, Completed, Master's course

  • 1992.09

    Keio University, Graduate School, Division of Science and Engineeri, 化学専攻

    Graduate School, Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

  • 理学, Keio University, 1992.09

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Green sustainable chemistry and environmental chemistry

  • Nanotechnology/Materials / Bio chemistry (微生物変換)

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Research Keywords 【 Display / hide 】

  • プラスチック分解菌

  • 微生物

  • 有機化学

  • 遺伝子

  • 酵素

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Books 【 Display / hide 】

  • 基礎から学ぶケイミカルバイオロジー

    上村大輔、袖岡幹子、阿部孝宏、闐闐孝介、中村和彦、宮本憲二, 共立出版, 2016

  • Green Biocatalysis

    MIYAMOTO KENJI, Wiley, 2016

    Scope: Decarboxylation and racemization of unnatural compounds using artificial enzymes derived from arylmalonate decarboxylase

  • 有機合成実験法ハンドブック

    MIYAMOTO KENJI, 有機合成化学協会, 2015.12

    Scope: 特殊環境からの新規酵素のスクリーニング

  • Chemical Biology

    MIYAMOTO KENJI, INTECH, 2012.02

    Scope: 63-82

  • Practical Methods for Biocatalysis and Biotransformations 2

    Miyamoto, Kenji; Kourist, Robert; Yoshida, Shosuke; Ohta, Hiromichi, 2012

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Papers 【 Display / hide 】

  • Hydrophobization of a TIP60 Protein Nanocage for the Encapsulation of Hydrophobic Compounds

    Yamashita M., Kawakami N., Miyamoto K.

    ChemPlusChem （ChemPlusChem)  88 （ 3 ）  2023.03

    　View Summary

    Encapsulation of hydrophobic molecules in protein-based nanocages is a promising approach for dispersing these molecules in water. Here, we report a chemical modification approach to produce a protein nanocage with a hydrophobic interior surface based on our previously developed nanocage, TIP60. The large pores of TIP60 act as tunnels for small molecules, allowing modification of the interior surface by hydrophobic compounds without nanocage disassembly. We used four different hydrophobic compounds for modification. The largest modification group tested, pyrene, resulted in a modified TIP60 that could encapsulate aromatic photosensitizer zinc phthalocyanine (ZnPC) more efficiently than the other modification compounds. The encapsulated ZnPC generated singlet oxygen upon light activation in the aqueous phase, whereas ZnPC alone formed inert aggregates under the same experimental conditions. Given that chemical modification allows a wider diversity of modifications than mutagenesis, this approach could be used to develop more suitable nanocages for encapsulating hydrophobic molecules of interest.

  • Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions

    Ohara N., Kawakami N., Arai R., Adachi N., Moriya T., Kawasaki M., Miyamoto K.

    Journal of the American Chemical Society （Journal of the American Chemical Society)  145 （ 1 ） 216 - 223 2023.01

    ISSN  00027863

    　View Summary

    Protein nanocages are of increasing interest for use as drug capsules, but the encapsulation and release of drug molecules at appropriate times require the reversible association and dissociation of the nanocages. One promising approach to addressing this challenge is the design of metal-dependent associating proteins. Such designed proteins typically have Cys or His residues at the protein surface for connecting the associating proteins through metal-ion coordination. However, Cys and His residues favor interactions with soft and borderline metal ions, such as Au+ and Zn2+, classified by the hard and soft acids and bases concept, restricting the types of metal ions available to drive association. Here, we show the alkaline earth (AE) metal-dependent association of the recently designed artificial protein nanocage TIP60, which is composed of 60-mer fusion proteins. The introduction of a Glu (hard base) mutation to the fusion protein (K67E mutant) prevented the formation of the 60-mer but formed the expected cage structure in the presence of Ca, Sr, or Ba ions (hard acids). Cryogenic electron microscopy (cryo-EM) analysis indicated a Ba ion at the interface of the subunits. Furthermore, we demonstrated the encapsulation and release of single-stranded DNA molecules using this system. Our results provide insights into the design of AE metal-dependent association and dissociation mechanisms for proteins.

  • Nanopore-Controlled Dual-Surface Modifications on Artificial Protein Nanocages as Nanocarriers

    Nasu E., Kawakami N., Miyamoto K.

    ACS Applied Nano Materials （ACS Applied Nano Materials)  4 （ 3 ） 2434 - 2439 2021.03

    　View Summary

    Chemical modification of the interior and exterior surfaces of protein nanocages holds promise for various applications such as cosmetics, pharmaceuticals, and catalysts. However, dual-surface modification of these surfaces using different chemicals remains challenging, particularly when the same substituents, such as cysteine thiols, are modified. We recently produced an artificial protein nanocage called TIP60 that has 20 large surface pores. Chemical modification of cysteine residues introduced in the interior surface of TIP60 showed that these pores allow the passage of small molecules from the outside environment to the inside of the nanocage. In this study, we found that the surface pores on TIP60 function as size-dependent molecular filters. Modification experiments using different-sized polymers containing maleimide groups, which specifically react with thiols, showed that macromolecules with diameters larger than that of the pores could not penetrate into the inner cavity. This molecular size discrimination by the pores prompted us to perform stepwise dual-surface functionalization of a double mutant of TIP60 presenting cysteine residues on the interior and exterior surfaces. This was achieved by modifying the exterior cysteine residues with a polymer containing a maleimide group that cannot penetrate to the inside of the nanocage, followed by modification of the interior cysteine residues using thiol-containing small molecules. Dual-functionalized TIP60 released internal small molecules in a redox-responsive manner. This simple approach for dual-surface modification would make TIP60 a useful nanocarrier for a broad range of applications including drug-delivery and molecular filtration systems.

  • Apoptosis-inducing activity and antiproliferative effect of Paeoniflorigenone from moutan cortex

    Ying Huang,Osamu Ohno, Kiyotake Suenaga, Kenji Miyamoto,

    Bioscience, Biotechnology, and Biochemistry  2017

    Joint Work, Accepted

  • 5-keto-4-deoxy-D-Glucarate Dehydratase/Decarboxylase: Key role in the oxidative pathway towards α-keto-glutarate

    André Pick, Barbara Beer, Risa Hemmi, Rena Momma, Jochen Schmid, Kenji Miyamoto and Volker Sieber

    BMC Biotechnology 16 （ 1 ）  2016.12

    Joint Work, Accepted

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • A method to improve enzyme thermostability using support vector machines

    Tsubamoto, Hitomi; Sakakibara, Yasubumi; Yoshida, Shosuke; Miyamoto, Kenji

    Bio Industry 30 ( 8 ) 12 - 19 2013.08

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

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Presentations 【 Display / hide 】

  • PET樹脂を分解する酵素の発見と利用

    MIYAMOTO KENJI

    慶應テクノモール (東京国際フォーラム) , 

    2017.12
    , 

    Oral presentation (invited, special)

  • PET樹脂を食べる微生物～その生い立ちと分解機構～

    MIYAMOTO KENJI

    化学最前線 (神奈川大学平塚キャンパス) , 

    2017.09
    , 

    Oral presentation (invited, special)

  • PET 分解菌の発見と分解機構の解析

    MIYAMOTO KENJI

    第１回慶應ライフサイエンスシンポジウム (慶應義塾大学協生館) , 

    2017.08
    , 

    Oral presentation (invited, special)

  • Thermally driven asymmetric decarboxylation using a thermostable esterase as a chiral space

    MIYAMOTO KENJI

    18th Japanese-German Workshop on Enzyme Technology, 

    2015.09
    , 

    Oral presentation (invited, special)

  • 酵素機能を合理的にデザインする

    MIYAMOTO KENJI

    公益社団法人　新化学技術推進協会ライフサイエンス技術部会・反応分科会　講演会, 

    2015.01
    , 

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 難分解性プラスチック分解菌の探索とその機能を利用した加工・リサイクル技術の開発

    2018.04
    -
    2023.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A) , Principal investigator

  • 好熱性酵素の活性部位を不斉反応場とした熱駆動型反応の実現

    2016.04
    -
    2017.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

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Intellectual Property Rights, etc. 【 Display / hide 】

  • スルホン酸エステル誘導体とその製造方法及びその利用法

    Date applied： 国際出願特許ＷＯ９８／０５６３４  1998 

    Patent, Joint

  • トリアゾール誘導体の製造法

    Date applied： 国際出願特許ＷＯ９５／２８３７４  1995 

    Patent, Joint

  • 芳香族ポリエステル分解酵素及び該酵素を用いた芳香族ポリエステル分解方法

    Date applied： 特願2015-532867   

    Patent, Joint

  • 光学活性4-(2-ハロ-1-ヒドロキシエチル)-2-トリフルオロメチルチアゾールの製造方法

    Date announced： 日本特許　特開平07-170996  1995 

    Patent, Joint

  • （Ｒ）－４－（２－ハロ－１－ヒドロキシエチル）－２－トリフルオロメチルチアゾールの製造方法

    Date announced： 日本特許　特開平07-265095  1995 

    Patent, Joint

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Awards 【 Display / hide 】

  • 第1回イムラ・ジャパン賞

    2017.02, 株式会社イム・ラジャパン

  • 第1回イムラジャパン賞

    宮本憲二, 2017.02, 株式会社イムラジャパン, PET分解酵素の活性向上に関する研究

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Courses Taught 【 Display / hide 】

  • TOPICS IN BIOSCIENCES AND INFORMATICS 1

    2023

  • SEMINAR IN BIOSCIENCES AND INFORMATICS

    2023

  • INTRODUCTION TO BIOLOGY TODAY

    2023

  • INTRODUCTION TO BIOLOGY

    2023

  • INDEPENDENT STUDY ON FUNDAMENTAL SCIENCE AND TECHNOLOGY

    2023

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Courses Previously Taught 【 Display / hide 】

  • 生化学

    慶應義塾大学理工学部

    2018.04
    -
    2019.03

  • 分子細胞生物学の基礎

    慶應義塾大学理工学部

    2018.04
    -
    2019.03

  • 自然化学実験

    慶應義塾大学理工学部

    2018.04
    -
    2019.03

  • 生体反応論第2

    慶應義塾大学

    2018.04
    -
    2019.03

  • 生物有機化学

    Keio University

    2017.04
    -
    2018.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

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Social Activities 【 Display / hide 】

  • Biocatalysis and Biotransformation

    2007.11
    -
    Present
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Memberships in Academic Societies 【 Display / hide 】

  • 酵素工学研究会, 

    2010.04
    -
    Present

  • 日本ケミカルバイオロジー学会, 

    2010.04
    -
    Present

  • 生体触媒化学研究会, 

    2009.04
    -
    Present

  • バイオインダストリー協会, 

    2007.10
    -
    Present

  • 有機合成化学協会, 

    1992.04
    -
    2014.03

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Committee Experiences 【 Display / hide 】

  • 2013.04
    -
    2015.03

    関東支部幹事, 日本農芸化学会

  • 2012.04
    -
    Present

    ATP小委員会員, 日本化学会

  • 2010.04
    -
    Present

    幹事, 新規素材探索研究会

  • 2010.04
    -
    Present

    会員, 日本ケミカルバイオロジー学会

  • 2010.04
    -
    Present

    Member, 酵素工学研究会

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