Matsubara, Teruhiko



Faculty of Science and Technology, Department of Biosciences and Informatics (Yagami)


Associate Professor

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Profile Summary 【 Display / hide

  • Design of peptide-based bionanomolecules for specific probes and inhibitors against oligosaccharide-related diseases including virus infection. Development of a new generation of chemical biology using contactless drops generated by acoustic levitation toward containerless processing.

Career 【 Display / hide

  • 1999.01

    日本学術振興会 特別研究員

  • 2000.07

    徳島大学工学部 助手

  • 2003.04


  • 2007.04


  • 2008.04


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Academic Background 【 Display / hide

  • 1995.03

    Okayama University, 工学部, 生体機能応用工学科

    University, Graduated

  • 1997.03

    Okayama University, Graduate School, Division of Engineering, 生体機能応用工学専攻

    Graduate School, Completed, Master's course

  • 2000.03

    Tokyo Institute of Technology, Graduate School, Division of Life Science and Engineering, バイオテクノロジー専攻

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • Ph.D., Tokyo Institute of Technology, Coursework, 2000.03


Research Areas 【 Display / hide

  • Nanotechnology/Materials / Bio chemistry (Chemistry Related to Living Body)

Research Keywords 【 Display / hide

  • ganglioside

  • phage display

  • peptide library

  • glycoconjugate

  • 音響浮揚

Proposed Theme of Joint Research 【 Display / hide

  • オリゴ糖鎖のクラスターに結合する分子の利用

    Interested in joint research with industry (including private organizations, etc.),  Desired form: Technical Consultation, Funded Research, Cooperative Research, Other

  • ファージ提示法による標的分子および物質に対するランダムライブラリーからのペプチドおよびタンパク質の選択

    Interested in joint research with other research organizations (including universities, etc.)


Books 【 Display / hide

  • ペプチド医薬品のスクリーニング・安定化・製剤化技術

    110名, 技術情報協会, 2017.12

    Scope: 6章1節

  • 生体分子化学-基礎から応用まで

    杉本 直己, 内藤 昌信, 橋詰 峰雄, 高橋 俊太郎, 田中 直毅, 建石 寿枝, 遠藤 玉樹, 津本 浩平, 長門石 曉, 松原 輝彦, 上田 実, 朝山 章一郎, 講談社サイエンティフィク, 2017.01

    Scope: 9章(p197-224)

  • 超分子サイエンス&テクノロジー

    松原 輝彦・佐藤智典, NTS, 2009.05

    Scope: 1036-1042

  • Contemporary Trends in Bacteriophage Research

    MATSUBARA TERUHIKO, Nova Science Publishers, Inc., 2009.05

    Scope: 407-419

  • 分子間相互作用解析ハンドブック

    松原輝彦・佐藤智典, 羊土社, 2007.09

    Scope: 16-22

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Papers 【 Display / hide

  • Detection of Influenza Virus by Agglutination of Microparticles Immobilized a Mixed Glycan Receptor Produced from Cells

    Matsubara T., Ogami A., Kori H., Hashizume M., Sato T.

    ACS Applied Bio Materials (ACS Applied Bio Materials)   2022.05

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    The hemagglutination inhibition (HAI) assay is one of the detection methods for influenza virus (IFV) under global influenza surveillance, which uses freshly prepared animal red blood cells (RBCs). Here, we demonstrate that a mixed glycan-modified polystyrene microparticle, which can be chemically prepared in advance, can replace animal RBCs in the HAI assay. A mixture of azide-conjugated glycans containing sialyl- and sulfated-lactose moieties was produced from Madin-Darby canine kidney (MDCK) cells, which are used for IFV isolation, and then immobilized on the surface of a polystyrene microparticle using click chemistry. Human HA and IFV were detected with high sensitivity when using the mixed glycan-immobilized particle.

  • De Novo Design of Star-Shaped Glycoligands with Synthetic Polymer Structures toward an Influenza Hemagglutinin Inhibitor

    Nagao M., Yamaguchi A., Matsubara T., Hoshino Y., Sato T., Miura Y.

    Biomacromolecules (Biomacromolecules)  23 ( 3 ) 1232 - 1241 2022.03

    ISSN  15257797

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    Synthetic polymers with well-defined structures allow the development of nanomaterials with additional functions beyond biopolymers. Herein, we demonstrate de novo design of star-shaped glycoligands to interact with hemagglutinin (HA) using well-defined synthetic polymers with the aim of developing an effective inhibitor for the influenza virus. Prior to the synthesis, the length of the star polymer chains was predicted using the Gaussian model of synthetic polymers, and the degree of polymerization required to achieve multivalent binding to three carbohydrate recognition domains (CRDs) of HA was estimated. The star polymer with the predicted degree of polymerization was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, and 6′-sialyllactose was conjugated as the glycoepitope for HA. The designed glycoligand exhibited the strongest interaction with HA as a result of multivalent binding. This finding demonstrated that the biological function of the synthetic polymer could be controlled by precisely defining the polymer structures.

  • Motobamide, an Antitrypanosomal Cyclic Peptide from a Leptolyngbya sp. Marine Cyanobacterium

    Takahashi H., Iwasaki A., Kurisawa N., Suzuki R., Jeelani G., Matsubara T., Sato T., Nozaki T., Suenaga K.

    Journal of Natural Products (Journal of Natural Products)  84 ( 5 ) 1649 - 1655 2021.05

    ISSN  01633864

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    Motobamide (1), a new cyclic peptide containing a C-prenylated cyclotryptophan residue, was isolated from a marine Leptolyngbya sp. cyanobacterium. Its planar structure was established by spectroscopic and MS/MS analyses. The absolute configuration was elucidated based on a combination of chemical degradations, chiral-phase HPLC analyses, spectroscopic analyses, and computational chemistry. Motobamide (1) moderately inhibited the growth of bloodstream forms of Trypanosoma brucei rhodesiense (IC50 2.3 μM). However, it exhibited a weaker cytotoxicity against normal human cells (IC50 55 μM).

  • Containerless Bioorganic Reactions in a Floating Droplet by Levitation Technique Using an Ultrasonic Wave

    Matsubara T., Takemura K.

    Advanced Science (Advanced Science)  8 ( 3 )  2021.02

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    To ensure sustainable consumption and production patterns, alternative process design without plastics for chemical and biological reactions will benefit future generations. Reaction flasks used in chemical and biological laboratories have been made from glass, metals, and plastics so far. If containerless processing can be realized, researchers will have a next-generation reaction process, which will be reactor and plastic-free, and without risks of unforeseen issues induced by contact with reactions flasks, including contamination and alteration of the reactants. Here, polymerization, click chemistry, and enzymatic reactions can proceed effectively in a floating droplet at a node of standing wave generated by ultrasonic levitation. These results demonstrate that floating droplets levitated by acoustic waves can represent a revolutionary containerless reactor for performing various reactions in the fields of chemistry and biology.

  • Heterogeneous Ganglioside-Enriched Nanoclusters with Different Densities in Membrane Rafts Detected by a Peptidyl Molecular Probe

    Matsubara T., Iijima K., Kojima T., Hirai M., Miyamoto E., Sato T.

    Langmuir (Langmuir)  37 ( 2 ) 646 - 654 2021.01

    ISSN  07437463

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    The specific features of the lateral distribution of gangliosides play key roles in cell-cell communications and the onset of various diseases related to the plasma membrane. We herein demonstrated that an artificial peptide identified from a phage-displayed library is available as a molecular probe for specific ganglioside nanoclustering sites in caveolae/membrane rafts on the cell surface. Atomic force microscopy studies indicated that the peptide specifically binds to the highly enriched monosialoganglioside GM1 nanodomains of reconstituted lipid bilayers composed of GM1, sphingomyelin, cholesterol, and unsaturated phospholipids. The ganglioside-containing area recognized by the peptide on the surface of PC12 cells was part of the area recognized by the cholera toxin B subunit, which has high affinity for GM1. Furthermore, the peptide bound to the cell surface after a treatment with methyl-β-cyclodextrin (MβCD), which disrupts membrane rafts by removing cholesterol. The present results indicate that there are heterogeneous ganglioside clusters with different ganglioside densities in caveolae/membrane rafts, and the peptidyl probe selectively recognizes the high-density ganglioside nanodomain that resists the MβCD treatment. This peptidyl probe will be useful for obtaining information on the lipid organization of the cell membrane and will help clarify the mechanisms by which the lateral distribution of gangliosides affects biological functions and the onset of diseases.

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Papers, etc., Registered in KOARA 【 Display / hide

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Development of target-oriented modification of peptide resources obtained by phage display method


    学術変革領域研究(A), Principal investigator

  • 全方位非接触界面による革新的バイオリアクターの開発


    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory), Principal investigator

  • Inhibition mechanism of modified peptide ligands that mimic complicated sugar receptors


    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

Awards 【 Display / hide

  • 東京糖鎖研究会(GlycoTOKYO)奨励賞

    MATSUBARA TERUHIKO, 2010.11, 東京糖鎖研究会(GlycoTOKYO), ライブラリーを用いた糖鎖-タンパク質間相互作用を制御するペプチドの設計

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • 若い世代の特別講演会(第23回)講演証

    MATSUBARA TERUHIKO, 2009.03, 日本化学会第89春季年会2009, ライブラリー選択法で得られた新規ペプチドによる細胞表面糖鎖の機能制御

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • バイオ関連化学合同シンポジウム講演賞

    MATSUBARA TERUHIKO, 2006.09, バイオ関連化学合同シンポジウム(日本化学会生体関連化学部会、バイオテクノロジー部会、生命化学研究会), ヘマグルチニン糖鎖結合ポケットを認識するペプチド分子設計

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • ISBC 2003 Poster Award

    Teruhiko Matsubara, 2003.12, First International Symposium on Biomolecular Chemistry, Functions of carbohydrate-binding peptides selected from phage-displayed peptide library

    Type of Award: Award from Japanese society, conference, symposium, etc.

     View Description

    First International Symposium on Biomolecular Chemistry (ISBC 2003), December 4, 2003


Courses Taught 【 Display / hide











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Memberships in Academic Societies 【 Display / hide

  • 日本ペプチド学会, 

  • 日本ソノケミストリー学会, 

  • 日本神経化学会, 

  • 日本糖質学会, 

  • 遺伝子・デリバリー研究会, 


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Committee Experiences 【 Display / hide

  • 2011.01

    ASSOCIATE EDITOR, Trends in Glycoscience and Glycotechnology

  • 2011.01

    Financial Secretary, FCCA