堀田 耕司 (ホッタ コウジ)

Hotta, Kohji

写真a

所属(所属キャンパス)

理工学部 生命情報学科 (矢上)

職名

准教授

HP

学歴 【 表示 / 非表示

  • 1997年03月

    北海道大学, 理学部

    大学, 卒業

  • 2002年03月

    京都大学, 理学研究科

    大学院, 修了, 博士

学位 【 表示 / 非表示

  • 博士(理学), 京都大学, 課程, 2002年03月

 

研究分野 【 表示 / 非表示

  • ライフサイエンス / 発生生物学 (Developmental Biology)

  • ライフサイエンス / 形態、構造

  • ライフサイエンス / 生物物理学

研究キーワード 【 表示 / 非表示

  • バイオイメージング

  • ホヤ

  • メカノバイオロジー

  • 進化発生

研究テーマ 【 表示 / 非表示

  • 尾芽胚の曲がりを支える3D形態ロジック, 

    2016年04月
    -
    2018年03月

  • 脊索動物尾芽伸長のロジック, 

    2013年
    -
    2014年

  • 個体まるごと用いた4次元イメージングによる形態形成運動の定量解析, 

    2009年
    -
    2011年

     研究概要を見る

     ナメクジウオ、ホヤ、ヒトはそれぞれ、頭索動物、尾索動物、脊椎動物に属し、これらは発生過程において脊索や、背側神経管と鰓孔、肛門より後方に伸びる尾など、共通のボディープランをもつ。このような共通の特長はどのようにしてつくられるのであろうか。脊索動物の発生過程は卵割期、胞胚期、原腸陥入期、神経板期、神経胚期、尾芽胚期といった共通の発

  • 脳胞形成の4次元定量解析, 

    2012年
    -
    2013年

     研究概要を見る

    Purpose of the Research Project

    Different region of central nervous system, fore brain, mid brain, hind brain and spinal cord originates from one neural tube at organogenesis period. Defects of neural tube formation cause severe diseases, including anencephaly and spina bifida. While understanding of differentiation at the molecular level has been deepened, the molecular mechanism of the morphogenesis of neural tubes at different regions remains to be clarified.

    One of chordate, ascidian a

  • ファイロティピック段階における形態の個体差とロバスト性, 

    2012年
    -
    2014年

 

論文 【 表示 / 非表示

  • Spatiotemporal dynamics of single cell stiffness in the early developing ascidian chordate embryo

    Fujii Y., Koizumi W.C., Imai T., Yokobori M., Matsuo T., Oka K., Hotta K., Okajima T.

    Communications Biology (Communications Biology)  4 ( 1 )  2021年12月

     概要を見る

    During the developmental processes of embryos, cells undergo massive deformation and division that are regulated by mechanical cues. However, little is known about how embryonic cells change their mechanical properties during different cleavage stages. Here, using atomic force microscopy, we investigated the stiffness of cells in ascidian embryos from the fertilised egg to the stage before gastrulation. In both animal and vegetal hemispheres, we observed a Rho kinase (ROCK)-independent cell stiffening that the cell stiffness exhibited a remarkable increase at the timing of cell division where cortical actin filaments were organized. Furthermore, in the vegetal hemisphere, we observed another mechanical behaviour, i.e., a ROCK-associated cell stiffening, which was retained even after cell division or occurred without division and propagated sequentially toward adjacent cells, displaying a characteristic cell-to-cell mechanical variation. The results indicate that the mechanical properties of embryonic cells are regulated at the single cell level in different germ layers.

  • Peripheral-neuron-like properties of differentiated human dental pulp stem cells (hDPSCs)

    Arimura Y., Shindo Y., Yamanaka R., Mochizuki M., Hotta K., Nakahara T., Ito E., Yoshioka T., Oka K.

    PLoS ONE (PLoS ONE)  16 ( 5 May )  2021年05月

     概要を見る

    Elucidating the mechanisms underlying human pain sensation requires the establishment of an in vitro model of pain reception comprising human cells expressing pain-sensing receptors and function properly as neurons. Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells and a promising candidate for producing human neuronal cells, however, the functional properties of differentiated hDPSCs have not yet been fully characterized. In this study, we demonstrated neuronal differentiation of hDPSCs via both their expression of neuronal marker proteins and their neuronal function examined using Ca2+ imaging. Moreover, to confirm the ability of nociception, Ca2+ responses in differentiated hDPSCs were compared to those of rat dorsal root ganglion (DRG) neurons. Those cells showed similar responses to glutamate, ATP and agonists of transient receptor potential (TRP) channels. Since TRP channels are implicated in nociception, differentiated hDPSCs provide a useful in vitro model of human peripheral neuron response to stimuli interpreted as pain.

  • High responsiveness of auditory neurons to specific combination of acoustic features in female songbirds

    Inda M., Hotta K., Oka K.

    European Journal of Neuroscience (European Journal of Neuroscience)  53 ( 5 ) 1412 - 1427 2021年03月

    ISSN  0953816X

     概要を見る

    Zebra finch (Taeniopygia guttata) is a songbird species in which males sing their unique songs to attract females who then select their preferred male. Acoustic features in the songs of individual males are important features for female auditory perception. While the male of this species is a classic model of vocal production, it has been little known about auditory processing in female. In the higher auditory brain regions, the caudomedial mesopallium (CMM) and nidopallium (NCM) contribute to female's sound recognition, we, therefore, extracted acoustic features that induce neural activities with high detection power on both regions in female finches. A multiple linear regression analysis revealed that neurons were sensitive to mean frequency and Wiener entropy. In addition, we performed an experiment with modified artificial songs and harmonic songs to directly investigate neural responsiveness for deriving further evidence for the contribution of these two acoustic features. Finally, we illustrated a specific ratio combining these two acoustic features that showed highest sensitivity to neural responsiveness, and we found that properties of sensitivity are different between CMM and NCM. Our results indicate that the mixture of the two acoustic features with the specific ratio is important in the higher auditory regions of female songbirds, and these two regions have differences in encoding for sensitivity to these acoustic features.

  • Two-Round Ca 2+ transient in papillae by mechanical stimulation induces metamorphosis in the ascidian Ciona intestinalis type A

    Wakai M.K., Nakamura M.J., Sawai S., Hotta K., Oka K.

    Proceedings of the Royal Society B: Biological Sciences (Proceedings of the Royal Society B: Biological Sciences)  288 ( 1945 )  2021年02月

    ISSN  09628452

     概要を見る

    Marine invertebrate larvae are known to begin metamorphosis in response to environmentally derived cues. However, little is known about the relationships between the perception of such cues and internal signalling for metamorphosis. To elucidate the mechanism underlying the initiation of metamorphosis in the ascidian, Ciona intestinalis type A (Ciona robusta), we artificially induced ascidian metamorphosis and investigated Ca 2+ dynamics from pre- to post-metamorphosis. Ca 2+ transients were observed and consisted of two temporally distinct phases with different durations before tail regression which is the early event of metamorphosis. In the first phase, Phase I, the Ca 2+ transient in the papillae (adhesive organ of the anterior trunk) was coupled with the Ca 2+ transient in dorsally localized cells and endoderm cells just after mechanical stimulation. The Ca 2+ transients in Phase I were also observed when applying only short stimulation. In the second phase, Phase II, the Ca 2+ transient in papillae was observed again and lasted for approximately 5-11 min just after the Ca 2+ transient in Phase I continued for a few minutes. The impaired papillae by Foxg-knockdown failed to induce the second Ca 2+ transient in Phase II and tail regression. In Phase II, a wave-like Ca 2+ propagation was also observed across the entire epidermis. Our results indicate that the papillae sense a mechanical cue and two-round Ca 2+ transients in papillae transmits the internal metamorphic signals to different tissues, which subsequently induces tail regression. Our study will help elucidate the internal mechanism of metamorphosis in marine invertebrate larvae in response to environmental cues.

  • Sexual and asexual development: two distinct programs producing the same tunicate

    Kowarsky M., Anselmi C., Hotta K., Burighel P., Zaniolo G., Caicci F., Rosental B., Neff N.F., Ishizuka K.J., Palmeri K.J., Okamoto J., Gordon T., Weissman I.L., Quake S.R., Manni L., Voskoboynik A.

    Cell Reports (Cell Reports)  34 ( 4 )  2021年01月

     概要を見る

    Colonial tunicates are the only chordate that possess two distinct developmental pathways to produce an adult body: either sexually through embryogenesis or asexually through a stem cell-mediated renewal termed blastogenesis. Using the colonial tunicate Botryllus schlosseri, we combine transcriptomics and microscopy to build an atlas of the molecular and morphological signatures at each developmental stage for both pathways. The general molecular profiles of these processes are largely distinct. However, the relative timing of organogenesis and ordering of tissue-specific gene expression are conserved. By comparing the developmental pathways of B. schlosseri with other chordates, we identify hundreds of putative transcription factors with conserved temporal expression. Our findings demonstrate that convergent morphology need not imply convergent molecular mechanisms but that it showcases the importance that tissue-specific stem cells and transcription factors play in producing the same mature body through different pathways.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

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総説・解説等 【 表示 / 非表示

研究発表 【 表示 / 非表示

  • BHF MEDIATES INDUCTION AND LOSS OF TOLERANCE IN Botryllus schlosseri CHIMERAS

    堀田 耕司

    North America Comparative Immunology Workshop 2017, 

    2017年06月

  • The complete annotation of Ciona development and anatomy: the larval and metamorphosis stages.

    堀田 耕司

    the Italian Embryology Group Meeting, 

    2017年06月

    口頭発表(一般)

  • Relationship between dynamics of cytoskeleton and cell stiffness in early ascidian embryo development.

    堀田 耕司

    The 22nd International Congress of Zoology, The 87th Meeting of The Zoological Society of Japan Joint Events in Okinawa, 

    2016年11月

  • In vivo-Imaging Analysis of Calcium Transients in Early Development of Ascidian Embryo.

    堀田 耕司

    The 22nd International Congress of Zoology, The 87th Meeting of The Zoological Society of Japan Joint Events in Okinawa (沖縄) , 

    2016年11月

    ポスター発表

  • Morphometry of tailbud embryo for understanding mechanism of conserved chordate phylotypic embryo shape.

    堀田 耕司

    The 22nd International Congress of Zoology, The 87th Meeting of The Zoological Society of Japan Joint Events in Okinawa, 

    2016年11月

    ポスター発表

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競争的研究費の研究課題 【 表示 / 非表示

  • ホヤ変態開始機構の定量解析

    2021年04月
    -
    2023年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 堀田 耕司, 新学術領域研究(研究領域提案型), 補助金,  研究代表者

  • 脳部位ごとの形態の違いを生み出す細胞構築機構の4D解析

    2016年04月
    -
    2019年03月

    独立行政法人日本学術振興会(JSPS), 科学研究費補助金(文部科学省・日本学術振興会), 堀田耕司, 補助金,  研究代表者

  • 尾芽胚の曲がりを支える3D形態ロジック

    2016年04月
    -
    2018年03月

    独立行政法人日本学術振興会(JSPS), 科学研究費補助金(文部科学省・日本学術振興会), 堀田耕司, 補助金,  研究代表者

受賞 【 表示 / 非表示

  • 武田科学財団 一般研究奨励

    堀田 耕司, 2008年11月, 脊索動物個体全体を用いた神経細胞ネットワークの網羅的解明

    受賞区分: 出版社・新聞社・財団等の賞

 

担当授業科目 【 表示 / 非表示

  • 生命情報特別講義第2

    2022年度

  • 生命情報輪講

    2022年度

  • 現代生物学概論

    2022年度

  • 生物学序論

    2022年度

  • 生物学実験集中

    2022年度

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担当経験のある授業科目 【 表示 / 非表示

  • 生物学序論

    慶應義塾

    2018年04月
    -
    2019年03月

    春学期, 講義, 専任, 2時間, 200人

    biology

  • 基礎生命実験

    慶應義塾

    2018年04月
    -
    2019年03月

    秋学期, 実習・実験

  • 発生生物学

    慶應義塾

    2018年04月
    -
    2019年03月

    秋学期, 講義, 専任, 30人

  • 生命情報実験

    慶應義塾

    2018年04月
    -
    2019年03月

    秋学期, 実習・実験, 専任, 40人

  • 細胞生物学第三

    慶應義塾

    2018年04月
    -
    2019年03月

    秋学期, 講義, 専任, 40人

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社会活動 【 表示 / 非表示

  • International Tunicate Information System Meeting

    2010年04月
    -
    継続中

所属学協会 【 表示 / 非表示

  • 日本遺伝学会

     
  • 日本動物学会

     
  • 日本発生生物学会

     
  • 日本進化学会

     
  • 日本分子生物学会

     

委員歴 【 表示 / 非表示

  • 2010年04月
    -
    継続中

    国際ホヤ情報システム会議委員, International Tunicate Information System Meeting