Fujiwara, Kei

写真a

Affiliation

Faculty of Science and Technology, Department of Biosciences and Informatics (Yagami)

Position

Associate Professor

Related Websites

External Links

Profile 【 Display / hide

  • Disrupted cells won't go back into its living states. We are trying to clarify why this process is irreversible and developing a method to reconstruct living cells from biomolecules mixtures using artificial cells engineering.

Career 【 Display / hide

  • 2009.04
    -
    2010.03

    The university of Tokyo, Graduate School of Frontier Sciences, Department of Medical Genome Sciences, JSPS research associate

  • 2010.04
    -
    2011.03

    Kyoto university, Institute for Integrated Cell-Material Sciences, Research Associate

  • 2011.04
    -
    2014.03

    Tohoku University, Department of Bioengineering and Robotics, JSPS research associate

Academic Background 【 Display / hide

  • 2004.04
    -
    2006.03

    The University of Tokyo, Graduate School of Agricultural and Life Sciences, Department of Applied Biological Chemistry

    Graduate School, Completed, Master's course

  • 2006.04
    -
    2009.03

    The University of Tokyo, Graduate School of Frontier Sciences, Department of Medical Genome Sciences

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(生命科学), The University of Tokyo, Coursework, 2009.03

    シャペロニンGroEL/ESの細胞内における役割の解明

 

Research Areas 【 Display / hide

  • Life Science / Molecular biology

  • Life Science / Biophysics

  • Life Science / System genome science

Research Keywords 【 Display / hide

  • Artificial-Cell engineering

  • Synthetic Biology

  • Bacteriology

  • Cell-free Life Sciences

 

Books 【 Display / hide

  • Synthetic Biology: A Very Short Introduction

    藤原 慶,徳永 美恵, ニュートンプレス, 2021.04

    Original author: Jamie A. Davies,

  • ペプチド医薬品のスクリーニング・安定化・製剤化技術

    SUDO Kei, FUJIWARA Kei, DOI Nobuhide, 技術情報協会, 2017.12

    Scope: 1-4. mRNAディスプレイ法によるペプチドのスクリーニング

  • 人工細胞の創製とその応用

    FUJIWARA Kei, CMC出版, 2017.01

    Scope: 「3-4. 無細胞システムによる生命システムの理解」の執筆

  • DNA分子デザインのすべて ~BIOMOD虎の巻

    FUJIWARA Kei, CBT学会eBOOK, 2016.04

    Scope: p. 15-20, p. 33-34の執筆を担当

     View Summary

    分子ロボティクス研究会編

  • 「自然世界の高分子」

    TANAKA Motohiko, TOKITA masayuki, YANAGISAWA Miho, SAKAUE Takahiro, FUJIWARA Kei, 吉岡書店, 2016.03

    Scope: 9章から11章の翻訳を担当

     View Summary

    「Giant molecules」Alexander Y. Grosberg and Alexei R. Khokhlovの邦訳版(翻訳)

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Papers 【 Display / hide

  • Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells

    Sakura Takada, Natsuhiko Yoshinaga, Nobuhide Doi, Kei Fujiwara

    Science Advances 8 ( 23 ) eabm8460 2022.06

    Research paper (scientific journal), Joint Work, Last author, Corresponding author, Accepted

  • Min波の人工細胞内再構成とそこから見えた細胞サイズ空間効果

    光山 隼史,義永 那津人,藤原 慶

    生物物理学会誌 (生物物理学会)  62 ( 1 ) 19 - 23 2022.03

    Accepted

  • Characterization of the membrane penetration-enhancing peptide S19 derived from human syncytin-1 for the intracellular delivery of TAT-fused proteins

    Suzuki M., Iwaki K., Kikuchi M., Fujiwara K., Doi N.

    Biochemical and Biophysical Research Communications (Biochemical and Biophysical Research Communications)  586   63 - 67 2022.01

    ISSN  0006291X

     View Summary

    Although cell-penetrating peptides such as the HIV-derived TAT peptide have been used as tools for the intracellular delivery of therapeutic peptides and proteins, a problem persists: the endosomal escape efficiency is low. Previously, we found that the fusogenic peptide S19, derived from the human protein syncytin-1, enhance the endosomal escape efficiency of proteins that incorporated by endocytosis via TAT. In this study, we first performed Ala-scanning mutagenesis of S19, and found that all Ile, Val, Leu and Phe with high β-sheet forming propensities in S19 are important for the intracellular uptake of S19-TAT-fused proteins. In a secondary structure analysis of the mutated S19-TAT peptides in the presence of liposomes mimicking late endosomes (LEs), the CD spectra of V3A and I4A mutants with low uptake activity showed the appearance of an α-helix structure, whereas the mutant G5A retained both the uptake activity and the β-structure. In addition, we investigated the appropriate linking position and order of the S19 and TAT peptides to a cargo protein including an apoptosis-induced peptide and found that both the previous C-terminal S19-TAT tag and the N-terminal TAT-S19 tag promote the cytoplasmic delivery of the fusion protein. These results and previous results suggest that the interaction of TAT with the LE membrane causes a structural change in S19 from a random coil to a β-strand and that the subsequent parallel β-sheet formation between two S19 peptides may promote adjacent TAT dimerization, resulting in endosomal escape from the LE membrane.

  • Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration

    Matsui Y., Akui T., Doi N., Fujiwara K.

    STAR Protocols (STAR Protocols)  2 ( 4 )  2021.12

     View Summary

    We present a protocol for activating protein synthesis in liposomes encapsulating a diluted E. coli cell extract-based TX-TL (transcription-translation) system by hypertonic concentration. Protein expression is turned on in the liposome-encapsulated TX-TL system by simple treatment with a concentrated external solution. The expression of sfGFP is demonstrated here, but it can be applied to other proteins. This protocol can be applied to the development of artificial cells utilizing the switch-on mechanism to activate protein expression, responding to the outer environment. For complete details on the use and execution of this protocol, please refer to Akui et al. (2021).

  • Transcription-translation of theEscherichia coligenome within artificial cells

    Deyama T., Matsui Y., Chadani Y., Sekine Y., Doi N., Fujiwara K.

    Chemical Communications (Chemical Communications)  57 ( 80 ) 10367 - 10370 2021.10

    ISSN  13597345

     View Summary

    Here we created artificial cells in which information of the genome of living cells is expressed by the elements encoded in the genome. We confirmed that the system works normally within artificial cells, which paves the way for reconstructing living cells from biomolecules.

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Papers, etc., Registered in KOARA 【 Display / hide

Reviews, Commentaries, etc. 【 Display / hide

  • 酵素を内包した人工細胞が拓く新しい醸造業や医薬品の開発

    藤原慶

    MDB 技術予測レポート    項目36 2019

  • Artificial cell fermentation as a next platform for biosynthesis

    FUJIWARA Kei

    バイオサイエンスとインダストリー ((一財)バイオインダストリー協会)  76 ( 4 ) 302 - 303 2018.07

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

  • DNA ナノテクノロジー コラム ”BIOMODへ参加しよう!”

    FUJIWARA Kei, Tadakuma Hisashi

    現代化学 (東京化学同人)   ( 11 ) 36 - 37 2016.10

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

  • Microdroplets as a Model System for the Study of Macromolecular Crowding in Cells

    YANAGISAWA Miho, FUJIWARA Kei

    生物物理 (日本生物物理学会)  55 ( 5 ) 246 - 249 2015.09

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • Reconstitution of intracellular environments in vitro and in artificial cells

    FUJIWARA Kei, Yanagisawa Miho, Nomura M. Shin-ichiro

    BIOPHYSICS (The Biophysics Society of Japan)  10   43 - 48 2014.08

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

Presentations 【 Display / hide

  • 細胞を創る:細胞分裂面決定機構の人工細胞内再構成

    FUJIWARA Kei

    第91回 日本生化学会大会 (京都国際会館) , 

    2018.09

    Oral presentation (invited, special)

  • 細胞サイズの微小液滴表面におけるMinタンパク質波の特異的な振る舞い

    FUJIWARA Kei

    第69回コロイドおよび界面化学討論会 (筑波大学) , 

    2018.09

    Oral presentation (invited, special)

  • 細胞を壊し、細胞を創る:創ることによる生命の理解

    FUJIWARA Kei

    第58回生物物理若手の会 夏の学校 (ぎふ長良川温泉パーク) , 

    2018.08

    Oral presentation (invited, special)

  • A localization wave of proteins reconstituted in artificial cells with crowding environments

    FUJIWARA Kei

    第55回生物物理学会年会 (熊本大学) , 

    2017.09

    Oral presentation (invited, special)

  • Behavior and characters of Min protein localization wave in cell-sized space

    FUJIWARA Kei

    アクティブマターの概念で繋ぐ生命機能の階層性 (グリーンピア大沼) , 

    2017.09

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • 細胞内にチューリングパターンは形成可能か?

    2022.06
    -
    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 挑戦的研究(萌芽), Principal investigator

  • 社会で実用可能な超越分子システムとしての人工細胞発酵法の確立

    2022.06
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    2024.03

    文部科学省・日本学術振興会, 科研費, No Setting

  • Establishment of artificial cell fermentation technology as a practical material for industrial usage

    2022.06
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    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 学術変革領域研究(A), Principal investigator

  • 細胞反応拡散波が示す情報・力学変換の理解

    2022.04
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    2024.03

    文部科学省・日本学術振興会, 科学研究費助成事業, Principal investigator

  • Conversion between mechanical power and information of intracellular reaction-diffusion wave

    2022.04
    -
    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 新学術領域研究(研究領域提案型), Principal investigator

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Courses Taught 【 Display / hide

  • ADVANCED LABORATORY COURSE IN BIOSCIENCES AND INFORMATICS A

    2022

  • TOPICS IN BIOSCIENCES AND INFORMATICS 2

    2022

  • SEMINAR IN BIOSCIENCES AND INFORMATICS

    2022

  • MICROBIOLOGY

    2022

  • LABORATORY IN SCIENCE

    2022

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Courses Previously Taught 【 Display / hide

  • 微生物学

    慶應義塾大学

    2018.04
    -
    2019.03