Sudo, Ryo

写真a

Affiliation

Faculty of Science and Technology, Department of System Design Engineering (Yagami)

Position

Professor

Related Websites

Career 【 Display / hide

  • 2005.04
    -
    2006.09

    慶應義塾大学 理工学部 特別研究助手

  • 2006.10
    -
    2009.03

    Postdoctoral Associate, Department of Biological Engnineering, Massachusetts Institute of Technology, USA

  • 2009.04
    -
    2012.03

    Assistant Professor, Department of System Design Engineering, Keio University

  • 2012.04
    -
    Present

    慶應義塾大学 理工学部 システムデザイン工学科 准教授

Academic Background 【 Display / hide

  • 2000.03

    Keio University, Faculty of Science and Engineering, Department of System Design Engineering

    University, Graduated

  • 2002.03

    Keio University, 理工学研究科, 基礎理工学専攻

    Graduate School, Completed, Master's course

  • 2005.03

    Keio University, 理工学研究科, 基礎理工学専攻

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • PhD, Keio University, 2005.03

 

Research Areas 【 Display / hide

  • Biomedical engineering/Biomaterial science and engineering (Biomedical Engineering/Tissue Engineering/Cell Biomechanics)

Research Keywords 【 Display / hide

  • Tissue Engineering

  • Cell biomechanics

  • BioMEMS

  • Microfluidic device

 

Books 【 Display / hide

  • “Hepatic Stem Cells”, Book Series “Methods in Molecular Biology”

    Ryo Sudo (Editor, Naoki Tanimizu), Springer, 2018.12

    Scope: Chapter 15 Reconstruction of hepatic tissue structures using interstitial flow in a microfluidic device,  Contact page: 167-174

  • 臓器チップの技術と開発動向

    須藤 亮(酒井 康行, 金森 敏幸 監修), シーエムシー出版, 2018.04

    Scope: 第7章 マイクロ流体システムによる血管形成モデルと肝細胞3次元培養モデルの融合,  Contact page: 193-200

  • 細胞のマルチスケールメカノバイオロジー

    谷下 一夫、須藤 亮(佐藤 正明 編著), 森北出版, 2017.05

    Scope: 第6章 細胞の力学刺激にともなう器官形成,  Contact page: 119-149

  • Vascular Engineering

    Ryo Sudo, Seok Chung, Yoojin Shin, Kazuo Tanishita (Editor, Kazuo Tanishita and Kimiko Yamamoto), Springer, 2016.03

    Scope: Chapter 16 Integrated Vascular Engineering: Vascularization of Reconstructed Tissue,  Contact page: 297-332

  • メカノバイオロジー

    阿部 順紀、須藤 亮、谷下 一夫(曽我部 正博 編著), 化学同人, 2015.08

    Scope: 23章 再生医工学におけるメカノバイオロジーIII:血管,  Contact page: 283-292

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Papers 【 Display / hide

  • Establishment of an in vitro vascular anastomosis model in a microfluidic device

    Masafumi Watanabe, Ryo Sudo

    Journal of Biomechanical Science and Engineering 14 ( 3 ) 18-00521 - (17 pages) 2019.11

    Research paper (scientific journal), Accepted

  • Construction of sinusoid-scale microvessels in perfusion culture of a decellularized liver

    Masafumi Watanabea, Koki Yano, Koki Okawa, Tadahiro Yamashita, Kazuki Tajima, Kazuaki Sawada, Hiroshi Yagi, Yuko Kitagawa, Kazuo Tanishita, Ryo Sudo

    Acta Biomaterialia (Acta Biomaterialia)  95   307 - 318 2019.09

    Research paper (scientific journal), Accepted,  ISSN  17427061

     View Summary

    © 2019 Acta Materialia Inc. There is a great deal of demand for the construction of transplantable liver grafts. Over the last decade, decellularization techniques have been developed to construct whole liver tissue grafts as potential biomaterials. However, the lack of intact vascular networks, especially sinusoids, in recellularized liver scaffolds leads to hemorrhage and thrombosis after transplantation, which is a major obstacle to the development of transplantable liver grafts. In the present study, we hypothesized that both mechanical (e.g., fluid shear stress) and chemical factors (e.g., fibronectin coating) can enhance the formation of hierarchical vascular networks including sinusoid-scale microvessels. We demonstrated that perfusion culture promoted formation of sinusoid-scale microvessels in recellularized liver scaffolds, which was not observed in static culture. In particular, perfusion culture at 4.7 ml/min promoted the formation of sinusoid-scale microvessels compared to perfusion culture at 2.4 and 9.4 ml/min. In addition, well-aligned endothelium was observed in perfusion culture, suggesting that endothelial cells sensed the flow-induced shear stress. Moreover, fibronectin coating of decellularized liver scaffolds enhanced the formation of sinusoid-scale microvessels in perfusion culture at 4.7 ml/min. This study represents a critical step in the development of functional recellularized liver scaffolds, which can be used not only for transplantation but also for drug screening and disease-modeling studies. Statement of Significance: Decellularized liver scaffolds are promising biomaterials that allow production of large-scale tissue-engineered liver grafts. However, it is difficult to maintain recellularized liver grafts after transplantation due to hemorrhage and thrombosis. To overcome this obstacle, construction of an intact vascular network including sinusoid-scale microvessels is essential. In the present study, we succeeded in constructing sinusoid-scale microvessels in decellularized liver scaffolds via a combination of perfusion culture and surface coating. We further confirmed that endothelial cells in decellularized liver scaffolds responded to flow-derived mechanical stress by aligning actin filaments. Our strategy to construct sinusoid-scale microvessels is critical for the development of intact vascular networks, and addresses the limitations of recellularized liver scaffolds after transplantation.

  • Balance of interstitial flow magnitude and vascular endothelial growth factor concentration modulates three-dimensional microvascular network formation

    Yoshinori Abe, Masafumi Watanabe, Seok Chung, Roger D. Kamm, Kazuo Tanishita, Ryo Sudo

    APL Bioengineering 3 ( 3 ) 036102 - (12 pages) 2019.07

    Research paper (scientific journal), Accepted

  • Self-organization of hepatocyte morphogenesis depending on the size of collagen microbeads relative to hepatocytes

    Mohammad Ajoudanian, Keita Enomoto, Yasuaki Tokunaga, Hiroshi Minami, Seok Chung, Kazuo Tanishita, Roger D. Kamm, Ryo Sudo

    Biofabrication (Biofabrication)  11 ( 3 ) 035007 - (11 pages) 2019.04

    Research paper (scientific journal), Accepted,  ISSN  17585082

     View Summary

    © 2019 IOP Publishing Ltd. Recent advances in microfabrication technologies have enabled us to construct collagen gel microbeads, which can be cultured with hepatocytes. However, little is known about the hepatocyte-collagen gel microbead interactions. Here, we aimed to clarify the effects of the balance between cell-cell and cell-collagen gel microbead interactions on hepatocyte morphogenesis and functions. The magnitude of cell-microbead interactions was controlled by changing the size of the microbeads, which were smaller than, comparable to, and larger than hepatocytes. These small, medium, and large microbeads were cultured separately with primary hepatocytes. Phase-contrast and time-lapse imaging revealed that the medium microbeads significantly induced the construction of 3D structures composed of the microbeads and hepatocytes in a self-organizing manner, whereas hepatocytes formed 2D monolayers with the small or large microbeads. These results suggest that only the medium microbeads induced the 3D tissue formation of hepatocytes. Furthermore, liver-specific functions, such as albumin secretion and ammonia clearance, were significantly upregulated in the 3D structures. These findings are critical to understand how to control the construction of 3D hepatocyte tissues with hydrogel microbeads in the context of biofabrication.

  • Comparison of organ-specific endothelial cells in terms of microvascular formation and endothelial barrier functions

    Hiroyuki Uwamori, Yuuichi Ono, Tadahiro Yamashita, Ryo Sudo

    Microvascular Research (Microvascular Research)  122   60 - 70 2019.03

    Research paper (scientific journal), Accepted,  ISSN  00262862

     View Summary

    © 2018 Elsevier Inc. Every organ demonstrates specific vascular characteristics and functions maintained by interactions of endothelial cells (ECs) and parenchymal cells. Particularly, brain ECs play a central role in the formation of a functional blood brain barrier (BBB). Organ-specific ECs have their own morphological features, and organ specificity must be considered when investigating interactions between ECs and other cell types constituting a target organ. Here we constructed angiogenesis-based microvascular networks with perivascular cells in a microfluidic device setting by coculturing ECs and mesenchymal stem cells (MSCs). Furthermore, we analyzed endothelial barrier functions as well as fundamental morphology, an essential step to build an in vitro BBB model. In particular, we used both brain microvascular ECs (BMECs) and human umbilical vein ECs (HUVECs) to test if organ specificity of ECs affects the formation processes and endothelial barrier functions of an engineered microvascular network. We found that microvascular formation processes differed by the source of ECs. HUVECs formed more extensive microvascular networks compared to BMECs while no differences were observed between BMECs and HUVECs in terms of both the microvascular diameter and the number of pericytes peripherally associated with the microvasculatures. To compare the endothelial barrier functions of each type of EC, we performed fluorescence dextran perfusion on constructed microvasculatures. The permeability coefficient of BMEC microvasculatures was significantly lower than that of HUVEC microvasculatures. In addition, there were significant differences in terms of tight junction protein expression. These results suggest that the organ source of ECs influences the properties of engineered microvasculature and thus is a factor to be considered in the design of organ-specific cell culture models.

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Papers, etc., Registered in KOARA 【 Display / hide

Research Projects of Competitive Funds, etc. 【 Display / hide

  • 血管相互作用を基軸にした三次元コンプレックス組織工学の創生

    2019.04
    -
    2023.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 須藤 亮, Grant-in-Aid for Scientific Research (B), Principal Investigator

  • 血管新生と神経新生の融合による三次元脳組織工学の開拓

    2018.06
    -
    2020.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 須藤 亮, Grant-in-Aid for Challenging Research (Exploratory) , Principal Investigator

  • 流れ制御を基軸にした血管化組織工学

    2016.04
    -
    2019.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 須藤 亮, Grant-in-Aid for Scientific Research (B), Principal Investigator

  • Microbiomechanics of glioma stem cells representing tumor heterogeneity in their invasion process

    2015.04
    -
    2017.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 須藤 亮, Grant-in-Aid for Challenging Exploratory Research, Principal Investigator

Awards 【 Display / hide

  • 生体医工学シンポジウム ベストレビューワーアワード

    2017.09, 日本生体医工学会

  • 平成28年度 科研費 審査委員表彰

    2016.09, 日本学術振興会

  • 2015年度 日本機械学会賞(論文)

    山本興子、谷村耕平、馬渕洋、松崎有未、Seok Chung、Roger D. Kamm、池田満里子、谷下一夫、須藤亮, 2016.04, 日本機械学会

  • Journal of Biomechanical Science and Engineering, 2013 Papers of the Year

    2014.08

  • Journal of Biomechanical Science and Engineering, 2013 Graphics of the Year

    2014.08

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Courses Taught 【 Display / hide

  • THERMOFLUID DYNAMICS 2

    2019

  • SYSTEM LIFE ENGINEERING

    2019

  • SEMINAR IN SYSTEM DESIGN ENGINEERING

    2019

  • MICRODEVICE SYSTEM DESIGN

    2019

  • LABORATORIES IN SYSTEM DESIGN ENGINEERING 2)

    2019

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Memberships in Academic Societies 【 Display / hide

  • 日本機械学会, 

    2005.01
    -
    Present
  • 肝細胞研究会, 

    2009.06
    -
    Present
  • 日本再生医療学会, 

    2009.06
    -
    Present
  • 日本バイオレオロジー学会, 

    2009.11
    -
    Present
  • 日本生体医工学会, 

    2011.04
    -
    Present

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Committee Experiences 【 Display / hide

  • 2014.04
    -
    Present

    Associate Editor, Journal of Biomechanical Science and Engineering

  • 2011.12
    -
    Present

    Board of Councillors, 日本バイオレオロジー学会

  • 2011.04
    -
    Present

    バイオメカニクス研究会 幹事, 日本生体医工学会

  • 2009.12
    -
    Present

    Committee Member, 日本バイオレオロジー学会