Fujimoto, Keiji

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry （Yagami）

Position

Professor

External Links
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Career 【 Display / hide 】

  • 1990.04
    -
    1996.03

    慶應義塾大学（理工学部） ,助手

  • 1994.09
    -
    1995.08

    米国マサチューセッツ州立大学訪問研究員

  • 1996.04
    -
    1999.03

    慶應義塾大学（理工学部） ,専任講師

  • 1999.04
    -
    2008.03

    慶應義塾大学（理工学部） ,助教授

  • 2000.04
    -
    2002.03

    大学院学習指導副主任（物質科学専攻）

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Academic Background 【 Display / hide 】

  • 1985.03

    Kyoto University, Faculty of Engineering, 高分子化学科

    University, Graduated

  • 1987.03

    Kyoto University, 工学部工学研究科, 高分子化学専攻

    Graduate School, Completed, Master's course

  • 1990.03

    Kyoto University, 工学部工学研究科, 高分子化学専攻

    Graduate School, Withdrawal after completion of doctoral course requirements, Doctoral course

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Academic Degrees 【 Display / hide 】

  • 京都大学博士（工学）, Kyoto University, Coursework, 1993.03

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Licenses and Qualifications 【 Display / hide 】

  • 昭和６２年国家公務員採用１種, 1987

  • 昭和６３年国家公務員採用１種, 1988

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Research Areas 【 Display / hide 】

  • Biomedical engineering/Biomaterial science and engineering (Nanobiomaterials)

  • Biomedical engineering/Biomaterial science and engineering

  • Nanobioscience (Nano Materials/Nano Bioscience)

  • Polymer chemistry (Polymer Science and Engineering)

  • Bio-related chemistry (Chemistry Related to Living Body)

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Research Keywords 【 Display / hide 】

  • エコマテリアル

  • ソフトマテリアル

  • ドラッグデリバリーシステム

  • ナノコーティング

  • バイオベースポリマー

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Proposed Theme of Joint Research 【 Display / hide 】

  • リポナノカプセル

  • 微粒子組織体

  • 細胞表層改質

  • 組織マテリアル工学

  • 高分子ゲル粒子の作製と機能化

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Books 【 Display / hide 】

  • 高分子微粒子のつくり方と活かし方

    藤本啓二、福井有香, 塗装工学 (一般社団法人）日本塗装技術協会) 54 ( 11 ) , 2019.11,  Page： 394 - 403

  • 高分子微粒子ハンドブック

    FUJIMOTO KEIJI, シーエムシー出版, 2017

    Scope: 高分子微粒子とは, 1-14

  • 高分子微粒子の創りかたと医用高分子への活かしかた

    FUJIMOTO KEIJI, 工業材料, 2017

  • 高分子微粒子ハンドブック

    FUJIMOTO KEIJI, シーエムシー出版, 2017

    Scope: コアシェル微粒子の作製と機能化, 167-175

  • 高分子微粒子ハンドブック

    FUJIMOTO KEIJI, シーエムシー出版, 2017

    Scope: ポリマー鎖の会合によって微粒子を作る, 114-121

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Papers 【 Display / hide 】

  • Creation of porous polymeric membranes by accumulation of water nanodroplets in a miniemulsion system

    Fukui Y., Fujino R., Sugaya Y., Fujimoto K.

    Polymer Journal （Polymer Journal)   2020.05

    Research paper (scientific journal), Accepted,  ISSN  00323896

    　View Summary

    © 2020, The Society of Polymer Science, Japan. We developed a miniemulsion templating method to prepare porous polymeric membranes. First, water nanodroplets were suspended in an oil phase by using a nonionic and polymeric surfactant to form a water-in-oil (W/O) miniemulsion. After the nanodroplets accumulated by centrifugation, a small amount of monomer was added as an oil phase to resuspend the water nanodroplets in a monomer phase. Then, photopolymerization of the monomer phase was conducted to generate pores in the polymeric matrix. The size of the nanodroplets was tuned by the surfactant concentration to control the pore size of the membranes. We could produce pore morphologies such as closed-cellular, open-cellular, and bicontinuous structures by tuning the volume fraction of the nanodroplets. Alternatively, nanodroplets were accumulated by centrifugation, and then further surfactants were added to the monomer to suppress the coalescence of nanodroplets. This enabled us to generate a highly porous open-cellular structure while maintaining the size and spherical shape. Next, HAuCl4 was reduced by using the surfactant displayed at the inner surface of the pore wall as the reducing agent. Gold nanoparticles were produced in the inner pores of the polymeric membrane, showing coloration derived from local surface plasmon resonance.

  • Tuning of particle indentation by surface modification of polymer particles and substrates

    Fukui Y., Yamamoto K., Yamamoto T., Fujimoto K.

    Colloids and Surfaces A: Physicochemical and Engineering Aspects （Colloids and Surfaces A: Physicochemical and Engineering Aspects)  588 2020.03

    Research paper (scientific journal), Accepted,  ISSN  09277757

    　View Summary

    © 2019 Elsevier B.V. We studied the influence of the chemical properties of the polymer particle and the polymer substrate on particle indentation. The polymer particles were arrayed over the polymer substrate and heat treatment (annealing) was conducted above the glass transition temperature (Tg) of bulk polymer to indent polymer particles into the polymer substrate. Surface topologies of particle-indented surfaces were observed with a field-emission scanning electron microscope (FE-SEM) and the degree of particle indentation (DI) was evaluated with a scanning probe microscope (SPM). The particle-arrayed substrate was placed upside down and then heat treatment was carried out. It was found that there was no effect of gravity on particle indentation. To prepare polymer particles with different chemical properties, surface grafting of hydrophobic, hydrophilic, cationic and anionic polymer chains was carried out by iniferter-initiated living radical polymerization. We found that the indentation of these particles was influenced by the outermost surface of the particle. Next, to investigate the effect of the substrate surface on particle indentation, sulfonation of the polystyrene (PS) substrate was carried out with concentrated sulfuric acid. We found that the surface property of the substrate also influenced particle indentation. From these results, we concluded that particle indentation would be determined by the interaction between the particle surface and the substrate surface. Finally, we intended to analyze the viscoelastic property of the substrate surface by particle indentation. We could estimate Tg values of PS and polyvinyl chloride (PVC) substrates by observing the particle indentation at the different annealing temperatures.

  • Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation

    Fukui Y., Otsuka H., Fujimoto K.

    Polymer Journal （Polymer Journal)  51 （ 11 ） 1223 - 1230 2019

    Research paper (scientific journal), Accepted,  ISSN  00323896

    　View Summary

    © 2019, The Society of Polymer Science, Japan. We prepared biobased nanocapsules with enzymatic degradability, which were generated by the layer-by-layer deposition of enzymes and polypeptide over the liposomal surface. Here, we demonstrate two different systems based on the enzymatic degradation of polymer layers. First, the deposition of trypsin and polyarginine (PArg), which is cleavable by trypsin, was carried out over a negatively charged liposome. The enzymatic cleavage of PArg resulted in exposure of the lipid membrane, which facilitated release of the cargo. Next, we attempted to degrade the outer polymer layer of the multilayered capsule wall to display the inner polymer layer by enzymatic degradation. This approach enabled the accumulation and targeting of the nanocapsules through the affinity between the displayed polymer layer and the target hydroxyapatite (HAp). The polymer wall was constructed with an inner layer consisting of poly-L-glutamic acid (PGlu) and an outer layer consisting of trypsin and PArg onto the liposome. The degradation of the outer PArg by trypsin allowed the surface to display the inner PGlu, which has bone-targeting ability. In addition, the polymer wall was constructed from an inner layer of PArg and an outer layer of pepsin and PGlu. The degradation of the outer PGlu by pepsin led to inner PArg on the surface to achieve cell-penetrating activity.

  • Generation of mucin gel particles with self-degradable and -releasable properties

    Yuuka Fukui, Megumi Fukuda, Keiji Fujimoto

    J. Mater. Chem. B （RSC)  6 （ 5 ） 781 - 788 2018

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  20507518

    　View Summary

    © The Royal Society of Chemistry 2018. Herein, we focused on mucin, which is a large viscous glycoprotein in terms of materials science, and reported preparation of mucin gel particles and incorporation of enzymes to provide the particle with self-degradable and releasable properties. To expose the hydrophobic peptide cores, trimming of sugar moieties was carried out by β-elimination reaction under alkaline conditions (tMucin). Nano-sized tMucin particles were prepared by the assembly of tMucin with the aid of a cationic surfactant. Then, cross-linking of tMucin particles was carried out via heat treatment (annealing) to induce thermal aggregation of the polypeptide chains. The hydrodynamic diameter of tMucin particles reversibly changed in response to calcium ions. Next, in an attempt to render the particle degradable, lysozyme was incorporated into the tMucin particles for the hydrolysis of oligosaccharide chains. These particles were gradually degraded upon enzymatic cleavage of the mucin molecules, facilitating the release of their incorporated substances. Also, the degradation of the mucin particles and the release of lysozyme were tunable by environmental conditions, such as temperature and calcium ions, in addition to the degree of cross-linking of the particles.

  • Preparation of free-standing hybrid colloidal membranes via assembly of liponanocapsules

    Y. Fukui, S. Kameyama, K. Fujimoto

    J. Biomaterials Science, Polymer Edition （Taylor & Francis)  10-12 （ 10-12 ） 1010 - 1024 2017.07

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  09205063

    　View Summary

    © 2017 Informa UK Limited, trading as Taylor & Francis Group. We intended to create a free-standing and organic–inorganic hybrid colloidal membrane. The colloidal membrane was assembled from polymeric capsules, which were prepared by coating of polymer layers over a liposome (liponanocapsules). In this study, two approaches were employed for mineralization over the membrane with calcium phosphate (CaP) to control its mechanical robustness and biodegradability (hybrid bioscaffold). One approach was based on CaP deposition over the liponanocapsules and their assembly into the hybrid membrane. CaP deposition was conducted via the counter-diffusion of phosphate ions and calcium ions across the capsule wall to obtain hybrid nanocapsules. Then, free-standing hybrid membrane was obtained by utilizing hybrid nanocapsules as building blocks for drying-mediated assembly. The obtained hybrid membrane was degraded into individual nanocapsules and degradation could be tuned by the crystal structure of CaP. In another approach, the free-standing membrane was assembled from DNA-coated liponanocapsules and then the counter-diffusion of ions was carried out across each assembled nanocapsule for CaP mineralization. The mechanical robustness of the membrane was significantly improved and its degradation was suppressed by CaP mineralization. This is probably because mineral cross-linkages were formed in the interspace between each nanocapsule. Fluorescent substances could be incorporated in each nanocapsule of the membrane and their release could be tuned by the control in crystal properties of CaP.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • 酵素固定化ゲル微粒子を用いたミネラリゼーションシステムの構築

    小林 尚裕, 福井 有香, 藤本 啓二

    日本バイオマテリアル学会大会予稿集 (日本バイオマテリアル学会)  40回   466 - 466 2018.11

  • 酵素ナノオブジェクトを用いた多機能薄膜の創製

    溝口 昂太郎, 福井 有香, 藤本 啓二

    日本バイオマテリアル学会大会予稿集 (日本バイオマテリアル学会)  40回   451 - 451 2018.11

  • 液晶性部位を有するケラチンナノ粒子作製と機能化

    伊藤 香, 福井 有香, 藤本 啓二

    日本バイオマテリアル学会大会予稿集 (日本バイオマテリアル学会)  40回   404 - 404 2018.11

  • リポソーム内部におけるポリマー合成による機能性カプセルの開発

    杉本 光, 福井 有香, 藤本 啓二

    日本バイオマテリアル学会大会予稿集 (日本バイオマテリアル学会)  40回   176 - 176 2018.11

  • ムチンのポリマーグラフト化とバイオコーティング材料への応用

    徳生 ひかる, 福井 有香, 藤本 啓二

    日本バイオマテリアル学会大会予稿集 (日本バイオマテリアル学会)  40回   198 - 198 2018.11

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Presentations 【 Display / hide 】

  • 酵素の連鎖反応を用いたゲル微粒子への炭酸カルシウムの複合化

    落合達也、福井有香、藤本啓二

    第29回高分子ゲル研究討論会 (東工大、大岡山) , 2018.01, Oral Presentation(general), 高分子学会

  • PHEMA微粒子をコアとする重合型および積層型表面層の構築

    兼森智規、福井有香、藤本啓二

    第29回高分子ゲル研究討論会 (東工大、大岡山) , 2018.01, Poster (general), 高分子学会

  • コアシェル型粒子を用いた中空ゲル粒子の作製

    栗原俊介、福井有香、藤本啓二

    第29回高分子ゲル研究討論会 (東工大、大岡山) , 2018.01, Oral Presentation(general), 高分子学会

  • ポリマー積層化リポソーム（リポナノカプセル）の酵素分解による機能発現

    大塚日加里、福井有香、藤本啓二

    日本バイオマテリアル学会シンポジウム2017 (タワーホール船堀) , 2017.11, Oral Presentation(general), 日本バイオマテリアル学会

  • W/Oミニエマルションにおけるナノ水滴を反応場としたリン酸カルシウム複合化高分子微粒子の作製

    高林浩平、福井有香、藤本啓二

    第26回ポリマー材料フォーラム (大阪国際交流センター) , 2017.11, Poster (general), 高分子学会

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Works 【 Display / hide 】

  • 有機化学演習（高分子化学）

    FUJIMOTO KEIJI

    1991.04
    -
    Present
    , 

    Other, Joint

  • 応用化学実験第２

    FUJIMOTO KEIJI

    1991.04
    -
    Present
    , 

    Other, Joint

  • 自然科学実験（化学）

    FUJIMOTO KEIJI

    1991.04
    -
    Present
    , 

    Other, Joint

  • 応用高分子化学

    FUJIMOTO KEIJI

    1996.04
    -
    1999.03
    , 

    Other, Single

  • 天然高分子

    FUJIMOTO KEIJI

    1999.04
    -
    2000.03
    , 

    Other, Single

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Intellectual Property Rights, etc. 【 Display / hide 】

  • リポソームを鋳型とする中空ナノ粒子の作製方法

    Application No.： 特願2004-113635  2004.04 

    Patent, Joint, National application

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Awards 【 Display / hide 】

  • BEST POSTER PRIZE in 2010 MRS Fall Meeting

    FUJIMOTO KEIJI, 2010.12, MRS, Development of a particle nano-imprinting technique by core-shell particles

    Type of Award： International Academic Awards

  • スマートポリマー国際シンポジウム・ベストポスター賞

    藤本 啓二, 1998.06, スマートポリマー国際シンポジウム

  • 高分子学会研究奨励賞

    藤本 啓二, 1997.05, 高分子学会

  • 日本MRS学会研究奨励賞

    藤本 啓二, 1993.12, 日本MRS学会

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Other 【 Display / hide 】

  • 2018年01月

    　View Details

    慶大　表面が硬い層のナノ粒子開発　歯・骨の欠損治療に応用へ、日経産業新聞

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Courses Taught 【 Display / hide 】

  • SEMINAR IN APPLIED CHEMISTRY

    2020

  • REGENERATION BIOLOGY

    2020

  • POLYMER CHEMISTRY

    2020

  • LABORATORIES IN APPLIED CHEMISTRY D

    2020

  • INDEPENDENT STUDY ON FUNDAMENTAL SCIENCE AND TECHNOLOGY

    2020

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Courses Previously Taught 【 Display / hide 】

  • 高分子化学基礎

    Keio University, 2018, Autumn Semester, General education subject, Lecture, Within own ｆaculty, 200people

  • バイオマテリアル特論

    Keio University, 2018, Spring Semester, Major subject, Lecture, Within own ｆaculty, 40people

  • 高分子化学

    Keio University, 2018, Spring Semester, Major subject, Lecture, Within own ｆaculty, 135people

  • 応用高分子化学

    慶應義塾大学, 2017

  • 天然高分子

    Keio Univiersity, 1999, Spring Semester, Major subject, Lecture, Within own ｆaculty

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Memberships in Academic Societies 【 Display / hide 】

  • 高分子学会, 

    2011.04
    -
    Present

  • 日本バイオマテリアル学会, 

    2002
    -
    Present

  • 高分子学会機関誌「高分子」, 

    1997.06
    -
    1999.05

  • 日本DDS学会

    　
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Committee Experiences 【 Display / hide 】

  • 2011.04
    -
    Present

    代表会員, 高分子学会

  • 2002
    -
    Present

    評議委員, 日本バイオマテリアル学会

  • 1997.06
    -
    1999.05

    編集委員, 高分子学会機関誌「高分子」

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