Fukui, Yuuka

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry （Yagami）

Position

Assistant Professor/Senior Assistant Professor

E-mail Address

E-mail address

Related Websites
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Career 【 Display / hide 】

  • 2015.04
    -
    2017.03

    慶應義塾大学, 理工学部　応用化学科, 助教

  • 2017.04
    -
    Present

    慶應義塾大学, 理工学部　応用化学科, 専任講師

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Academic Background 【 Display / hide 】

  • 2003.04
    -
    2007.03

    Keio University, 理工学部, 応用化学科

    University, Graduated

  • 2007.04
    -
    2009.03

    慶應義塾大学大学院, 理工学研究科, 生物化学専修

    Graduate School, Master's course

  • 2009.04
    -
    2012.03

    慶應義塾大学大学院, 理工学研究科, 生物化学専修

    Graduate School, Doctoral course

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Academic Degrees 【 Display / hide 】

  • 博士（工学）, Keio University, Coursework, 2012.03

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Books 【 Display / hide 】

  • ドラッグデリバリーシステム・バイオ医薬品創成に向けた組織、細胞内、核内送達技術の開発

    福井有香, 藤本啓二, シーエムシー出版, 2018.08

    Scope: リポソームの表面改質によるナノカプセルの作製と機能化,  Contact page： 195-206

  • 中空微粒子の合成と応用

    FUKUI YuukaFUJIMOTO Keiji, シーエムシー出版, 2017

    Scope: リポソームを鋳型とした中空微粒子の合成

  • 高分子微粒子ハンドブック

    FUKUI Yuuka, シーエムシー出版, 2017

    Scope: ミニエマルション重合

  • 高分子微粒子ハンドブック

    FUKUI Yuuka, シーエムシー出版, 2017

    Scope: ミネラル架橋による微粒子の作製

  • Encyclopedia of Biocolloid and Biointerface Science

    FUKUI YuukaFUJIMOTO Keiji, Wiley, Inc., 2016

    Scope: "Liponano-capsule: a Nanocapsule Built from a Liposomal Template", Chapter 56, 684-689

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Papers 【 Display / hide 】

  • Preparation of Bionanocapsules by the Layer-by-Layer Deposition of Polypeptides onto Liposome

    FUJIMOTO Keiji, TOYODA Tomonori, FUKUI Yuuka

    Macromolecules 40 （ 14 ） 5122-5128 2007.05

    Research paper (scientific journal), Joint Work, Except for reviews

  • Tuning of particle indentation by surface modification of polymer particles and substrates

    Fukui Y., Yamamoto K., Yamamoto T., Fujimoto K.

    Colloids and Surfaces A: Physicochemical and Engineering Aspects （Colloids and Surfaces A: Physicochemical and Engineering Aspects)  588 2020.03

    ISSN  09277757

    　View Summary

    © 2019 Elsevier B.V. We studied the influence of the chemical properties of the polymer particle and the polymer substrate on particle indentation. The polymer particles were arrayed over the polymer substrate and heat treatment (annealing) was conducted above the glass transition temperature (Tg) of bulk polymer to indent polymer particles into the polymer substrate. Surface topologies of particle-indented surfaces were observed with a field-emission scanning electron microscope (FE-SEM) and the degree of particle indentation (DI) was evaluated with a scanning probe microscope (SPM). The particle-arrayed substrate was placed upside down and then heat treatment was carried out. It was found that there was no effect of gravity on particle indentation. To prepare polymer particles with different chemical properties, surface grafting of hydrophobic, hydrophilic, cationic and anionic polymer chains was carried out by iniferter-initiated living radical polymerization. We found that the indentation of these particles was influenced by the outermost surface of the particle. Next, to investigate the effect of the substrate surface on particle indentation, sulfonation of the polystyrene (PS) substrate was carried out with concentrated sulfuric acid. We found that the surface property of the substrate also influenced particle indentation. From these results, we concluded that particle indentation would be determined by the interaction between the particle surface and the substrate surface. Finally, we intended to analyze the viscoelastic property of the substrate surface by particle indentation. We could estimate Tg values of PS and polyvinyl chloride (PVC) substrates by observing the particle indentation at the different annealing temperatures.

  • Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation

    Fukui Y., Otsuka H., Fujimoto K.

    Polymer Journal （Polymer Journal)  51 （ 11 ） 1223 - 1230 2019.11

    ISSN  00323896

    　View Summary

    © 2019, The Society of Polymer Science, Japan. We prepared biobased nanocapsules with enzymatic degradability, which were generated by the layer-by-layer deposition of enzymes and polypeptide over the liposomal surface. Here, we demonstrate two different systems based on the enzymatic degradation of polymer layers. First, the deposition of trypsin and polyarginine (PArg), which is cleavable by trypsin, was carried out over a negatively charged liposome. The enzymatic cleavage of PArg resulted in exposure of the lipid membrane, which facilitated release of the cargo. Next, we attempted to degrade the outer polymer layer of the multilayered capsule wall to display the inner polymer layer by enzymatic degradation. This approach enabled the accumulation and targeting of the nanocapsules through the affinity between the displayed polymer layer and the target hydroxyapatite (HAp). The polymer wall was constructed with an inner layer consisting of poly-L-glutamic acid (PGlu) and an outer layer consisting of trypsin and PArg onto the liposome. The degradation of the outer PArg by trypsin allowed the surface to display the inner PGlu, which has bone-targeting ability. In addition, the polymer wall was constructed from an inner layer of PArg and an outer layer of pepsin and PGlu. The degradation of the outer PGlu by pepsin led to inner PArg on the surface to achieve cell-penetrating activity.

  • Generation of mucin gel particles with self-degradable and -releasable properties

    Fukui, Yuuka, Fukuda, Megumi, Fujimoto, Keiji

    JOURNAL OF MATERIALS CHEMISTRY B （Journal of Materials Chemistry B)  6 （ 5 ） 781 - 788 2018.02

    Research paper (scientific journal), Joint Work,  ISSN  2050-750X

    　View Summary

    © The Royal Society of Chemistry 2018. Herein, we focused on mucin, which is a large viscous glycoprotein in terms of materials science, and reported preparation of mucin gel particles and incorporation of enzymes to provide the particle with self-degradable and releasable properties. To expose the hydrophobic peptide cores, trimming of sugar moieties was carried out by β-elimination reaction under alkaline conditions (tMucin). Nano-sized tMucin particles were prepared by the assembly of tMucin with the aid of a cationic surfactant. Then, cross-linking of tMucin particles was carried out via heat treatment (annealing) to induce thermal aggregation of the polypeptide chains. The hydrodynamic diameter of tMucin particles reversibly changed in response to calcium ions. Next, in an attempt to render the particle degradable, lysozyme was incorporated into the tMucin particles for the hydrolysis of oligosaccharide chains. These particles were gradually degraded upon enzymatic cleavage of the mucin molecules, facilitating the release of their incorporated substances. Also, the degradation of the mucin particles and the release of lysozyme were tunable by environmental conditions, such as temperature and calcium ions, in addition to the degree of cross-linking of the particles.

  • Creation of hybrid polymer particles through morphological tuning of CaCO3 crystals in miniemulsion system

    Fukui Yuuka, Takamatsu Hideyuki, Fujimoto Keiji

    Colloids and Surfaces A: Physicochemical and Engineering Aspects 516   1 - 8 2017.03

    ISSN  0927-7757

    　View Summary

    <p>We developed a miniemulsion-based nanoreactor system, which can allow for morphological tuning of CaCO3 crystals in nanodroplets and their high encapsulation efficiency in polymer particles. We first obtained nano-sized CaCO3 by using the aqueous nanodroplet as a nanoreactor. In addition, it was found that crystal growth and morphological transformation of CaCO3 from spherical to rod-like crystals were induced by adding 2-hydroxyethyl methacrylate (HEMA) in nanodroplets. Such transformation was also promoted by raising the incubation temperature or increasing the number of nanodroplets in the presence of HEMA. We speculated that crystal growth of CaCO3 crystals was induced by coalescence of nanodroplets and morphological transformation to rod-like shape was undertaken with an assistance of HEMA. In this way, we could tune the shapes of CaCO3 with the well-controlled size and aspect ratio by tuning environments in nanodroplets. Then, subsequent polymerization of HEMA, which was used as a monomer, was carried out for encapsulation of CaCO3 with rod-like morphologies inside polymer nanoparticles. As a result, spherical and spheroidal hybrid nanoparticles, which possessed homogeneity in size and shape, were obtained. Then, by spin-coating of hybrid nanoparticles onto the glass substrate, we could obtain a closely-packed particle monolayer.</p>

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • ミネラル層で覆われたリポナノカプセルの創製

    福井有香

    PHARM STAGE (技術情報協会 )  18 ( 5 )  2018.08

    Introduction and explanation (scientific journal), Single Work

  • 機能性高分子微粒子の合成と作製技術

    福井有香, 藤本啓二

    MATERIAL STAGE (技術情報協会 )  18 ( 2 ) 1 - 6 2018

    Introduction and explanation (scientific journal), Joint Work

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Presentations 【 Display / hide 】

  • Generation of Mucin Gel Particles with Self-degradable and -Releasable Properties

    Yuuka Fukui, Megumi Fukuda, Keiji Fujimoto

    15th International Hydrocolloids Conference (Melbourne, Australia) , 2020.03, Oral Presentation(general), Elsevier

    　View Summary

    Mucins, which are large viscous glycoproteins, have attracted many researchers to explore their potential applications in terms of materials science. In this work, we developed a strategy1 for the preparation of mucin nanoparticles and incorporation of enzymes to impart degradability and releasability. This aimed at applications of mucin gel nanoparticles mainly in the drug delivery system.

  • ゲル微粒子を用いた粘着性コロイド構造体の作製

    渡邉龍海,志樂貴大,福井有香,藤本啓二

    第28回ポリマー材料フォーラム (ウインク愛知) , 2019.11, Poster (general), 高分子学会

  • カテコール含有中空ゲル微粒子からなるコロイドゲル構造体の創製と機能化

    吉住彩里,平古場文也,福井有香,藤本啓二

    第28回ポリマー材料フォーラム (ウインク愛知) , 2019.11, Poster (general), 高分子学会

  • 微粒子の埋没による高分子表面の微細加工と機能化

    藤井祐介,山本卓明,福井有香,藤本啓二

    第28回ポリマー材料フォーラム (ウインク愛知) , 2019.11, Poster (general), 高分子学会

  • グラフト化タンパク質を用いたゲル材料の表面改質

    野田百恵,徳生ひかる,福井有香,藤本啓二

    第41回日本バイオマテリアル学会大会 (つくば国際会議場) , 2019.11, Poster (general), 日本バイオマテリアル学会

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 骨疾患治療のための骨環境応答性ナノツールの開発

    2019.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 福井　有香, Grant-in-Aid for Early-Career Scientists , Principal Investigator

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Awards 【 Display / hide 】

  • The Young Investigator Award Gold Prize(11th Liposome Research Days Conference)

    FUKUI Yuuka, 2008.07, Preparation of Lipo-nanocapsules by the layer-by-layer deposition of polysaccharides and coating of calcium phosphate onto a liposome

    Type of Award： Awards of National Conference, Council and Symposium

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Courses Taught 【 Display / hide 】

  • SEMINAR IN APPLIED CHEMISTRY

    2020

  • LABORATORIES IN SCIENCE AND TECHNOLOGY

    2020

  • LABORATORIES IN APPLIED CHEMISTRY D

    2020

  • FRONTIER OF CHEMISTRY

    2020

  • BACHELOR'S THESIS

    2020

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Memberships in Academic Societies 【 Display / hide 】

  • Materials Research Society, 

    2011.11
    -
    Present

  • American Chemical Society, 

    2009.05
    -
    Present

  • 日本バイオマテリアル学会, 

    2007.11
    -
    Present

  • 高分子学会, 

    2007.05
    -
    Present
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