KEIO RESEARCHERS INFORMATION SYSTEM

Career 【 Display / hide 】

Career 【 Display / hide 】

• 2015.04

  -

  2017.03

  慶應義塾大学, 理工学部　応用化学科, 助教

• 2017.04

  -

  Present

  慶應義塾大学, 理工学部　応用化学科, 専任講師

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 2003.04

  -

  2007.03

  Keio University, 理工学部, 応用化学科

  University, Graduated

• 2007.04

  -

  2009.03

  慶應義塾大学大学院, 理工学研究科, 生物化学専修

  Graduate School, Master's course

• 2009.04

  -

  2012.03

  慶應義塾大学大学院, 理工学研究科, 生物化学専修

  Graduate School, Doctoral course

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• 博士（工学）, Keio University, Coursework, 2012.03

Books 【 Display / hide 】

Books 【 Display / hide 】

• ドラッグデリバリーシステム・バイオ医薬品創成に向けた組織、細胞内、核内送達技術の開発

  福井有香, 藤本啓二, シーエムシー出版, 2018.08

  Scope: リポソームの表面改質によるナノカプセルの作製と機能化,  Contact page： 195-206

• 中空微粒子の合成と応用

  FUKUI YuukaFUJIMOTO Keiji, シーエムシー出版, 2017

  Scope: リポソームを鋳型とした中空微粒子の合成

• 高分子微粒子ハンドブック

  FUKUI Yuuka, シーエムシー出版, 2017

  Scope: ミニエマルション重合

• 高分子微粒子ハンドブック

  FUKUI Yuuka, シーエムシー出版, 2017

  Scope: ミネラル架橋による微粒子の作製

• Encyclopedia of Biocolloid and Biointerface Science

  FUKUI YuukaFUJIMOTO Keiji, Wiley, Inc., 2016

  Scope: "Liponano-capsule: a Nanocapsule Built from a Liposomal Template", Chapter 56, 684-689

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Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Preparation of Bionanocapsules by the Layer-by-Layer Deposition of Polypeptides onto Liposome

  FUJIMOTO Keiji, TOYODA Tomonori, FUKUI Yuuka

  Macromolecules 40 （ 14 ） 5122-5128 2007.05

  Research paper (scientific journal), Joint Work, Except for reviews

• Creation of porous polymeric membranes by accumulation of water nanodroplets in a miniemulsion system

  Fukui Y., Fujino R., Sugaya Y., Fujimoto K.

  Polymer Journal （Polymer Journal)  52 （ 9 ） 1077 - 1083 2020.09

  ISSN  00323896

  　View Summary

  © 2020, The Society of Polymer Science, Japan. We developed a miniemulsion templating method to prepare porous polymeric membranes. First, water nanodroplets were suspended in an oil phase by using a nonionic and polymeric surfactant to form a water-in-oil (W/O) miniemulsion. After the nanodroplets accumulated by centrifugation, a small amount of monomer was added as an oil phase to resuspend the water nanodroplets in a monomer phase. Then, photopolymerization of the monomer phase was conducted to generate pores in the polymeric matrix. The size of the nanodroplets was tuned by the surfactant concentration to control the pore size of the membranes. We could produce pore morphologies such as closed-cellular, open-cellular, and bicontinuous structures by tuning the volume fraction of the nanodroplets. Alternatively, nanodroplets were accumulated by centrifugation, and then further surfactants were added to the monomer to suppress the coalescence of nanodroplets. This enabled us to generate a highly porous open-cellular structure while maintaining the size and spherical shape. Next, HAuCl4 was reduced by using the surfactant displayed at the inner surface of the pore wall as the reducing agent. Gold nanoparticles were produced in the inner pores of the polymeric membrane, showing coloration derived from local surface plasmon resonance.

  • Access to Document (DOI)

• Tuning of particle indentation by surface modification of polymer particles and substrates

  Fukui Y., Yamamoto K., Yamamoto T., Fujimoto K.

  Colloids and Surfaces A: Physicochemical and Engineering Aspects （Colloids and Surfaces A: Physicochemical and Engineering Aspects)  588 2020.03

  ISSN  09277757

  　View Summary

  © 2019 Elsevier B.V. We studied the influence of the chemical properties of the polymer particle and the polymer substrate on particle indentation. The polymer particles were arrayed over the polymer substrate and heat treatment (annealing) was conducted above the glass transition temperature (Tg) of bulk polymer to indent polymer particles into the polymer substrate. Surface topologies of particle-indented surfaces were observed with a field-emission scanning electron microscope (FE-SEM) and the degree of particle indentation (DI) was evaluated with a scanning probe microscope (SPM). The particle-arrayed substrate was placed upside down and then heat treatment was carried out. It was found that there was no effect of gravity on particle indentation. To prepare polymer particles with different chemical properties, surface grafting of hydrophobic, hydrophilic, cationic and anionic polymer chains was carried out by iniferter-initiated living radical polymerization. We found that the indentation of these particles was influenced by the outermost surface of the particle. Next, to investigate the effect of the substrate surface on particle indentation, sulfonation of the polystyrene (PS) substrate was carried out with concentrated sulfuric acid. We found that the surface property of the substrate also influenced particle indentation. From these results, we concluded that particle indentation would be determined by the interaction between the particle surface and the substrate surface. Finally, we intended to analyze the viscoelastic property of the substrate surface by particle indentation. We could estimate Tg values of PS and polyvinyl chloride (PVC) substrates by observing the particle indentation at the different annealing temperatures.

  • Access to Document (DOI)

• Controlled release and targeting of polypeptide-deposited liposomes by enzymatic degradation

  Fukui Y., Otsuka H., Fujimoto K.

  Polymer Journal （Polymer Journal)  51 （ 11 ） 1223 - 1230 2019.11

  ISSN  00323896

  　View Summary

  © 2019, The Society of Polymer Science, Japan. We prepared biobased nanocapsules with enzymatic degradability, which were generated by the layer-by-layer deposition of enzymes and polypeptide over the liposomal surface. Here, we demonstrate two different systems based on the enzymatic degradation of polymer layers. First, the deposition of trypsin and polyarginine (PArg), which is cleavable by trypsin, was carried out over a negatively charged liposome. The enzymatic cleavage of PArg resulted in exposure of the lipid membrane, which facilitated release of the cargo. Next, we attempted to degrade the outer polymer layer of the multilayered capsule wall to display the inner polymer layer by enzymatic degradation. This approach enabled the accumulation and targeting of the nanocapsules through the affinity between the displayed polymer layer and the target hydroxyapatite (HAp). The polymer wall was constructed with an inner layer consisting of poly-L-glutamic acid (PGlu) and an outer layer consisting of trypsin and PArg onto the liposome. The degradation of the outer PArg by trypsin allowed the surface to display the inner PGlu, which has bone-targeting ability. In addition, the polymer wall was constructed from an inner layer of PArg and an outer layer of pepsin and PGlu. The degradation of the outer PGlu by pepsin led to inner PArg on the surface to achieve cell-penetrating activity.

  • Access to Document (DOI)

• Generation of mucin gel particles with self-degradable and -releasable properties

  Fukui, Yuuka, Fukuda, Megumi, Fujimoto, Keiji

  JOURNAL OF MATERIALS CHEMISTRY B （Journal of Materials Chemistry B)  6 （ 5 ） 781 - 788 2018.02

  Research paper (scientific journal), Joint Work,  ISSN  2050-750X

  　View Summary

  © The Royal Society of Chemistry 2018. Herein, we focused on mucin, which is a large viscous glycoprotein in terms of materials science, and reported preparation of mucin gel particles and incorporation of enzymes to provide the particle with self-degradable and releasable properties. To expose the hydrophobic peptide cores, trimming of sugar moieties was carried out by β-elimination reaction under alkaline conditions (tMucin). Nano-sized tMucin particles were prepared by the assembly of tMucin with the aid of a cationic surfactant. Then, cross-linking of tMucin particles was carried out via heat treatment (annealing) to induce thermal aggregation of the polypeptide chains. The hydrodynamic diameter of tMucin particles reversibly changed in response to calcium ions. Next, in an attempt to render the particle degradable, lysozyme was incorporated into the tMucin particles for the hydrolysis of oligosaccharide chains. These particles were gradually degraded upon enzymatic cleavage of the mucin molecules, facilitating the release of their incorporated substances. Also, the degradation of the mucin particles and the release of lysozyme were tunable by environmental conditions, such as temperature and calcium ions, in addition to the degree of cross-linking of the particles.

  • Access to Document (DOI)

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Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Development of liposome-based biomaterials for bone therapeutics

  Fukui, Yuka

  科学研究費補助金研究成果報告書 2013

Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• ミネラル層で覆われたリポナノカプセルの創製

  福井有香

  PHARM STAGE (技術情報協会 )  18 ( 5 )  2018.08

  Introduction and explanation (scientific journal), Single Work

• 機能性高分子微粒子の合成と作製技術

  福井有香, 藤本啓二

  MATERIAL STAGE (技術情報協会 )  18 ( 2 ) 1 - 6 2018

  Introduction and explanation (scientific journal), Joint Work

Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• Generation of Mucin Gel Particles with Self-degradable and -Releasable Properties

  Yuuka Fukui, Megumi Fukuda, Keiji Fujimoto

  15th International Hydrocolloids Conference (Melbourne, Australia) , 2020.03, Oral Presentation(general), Elsevier

  　View Summary

  Mucins, which are large viscous glycoproteins, have attracted many researchers to explore their potential applications in terms of materials science. In this work, we developed a strategy1 for the preparation of mucin nanoparticles and incorporation of enzymes to impart degradability and releasability. This aimed at applications of mucin gel nanoparticles mainly in the drug delivery system.

• グラフト鎖を有する微粒子を用いたコロイドゲルの構築と構造発色の調節

  深澤里羅,福井有香,藤本啓二

  第28回ポリマー材料フォーラム (ウインク愛知) , 2019.11, Poster (general), 高分子学会

• 酵素固定化ゲル微粒子による反応場の創成とミネラリゼーションへの応用

  小林尚裕,福井有香,藤本啓二

  第41回日本バイオマテリアル学会大会 (つくば国際会議場) , 2019.11, Oral Presentation(general), 日本バイオマテリアル学会

• コアシェル粒子の化学的改質によるコロイドスポンジへの機能付与

  大浦順,福井有香,藤本啓二

  第28回ポリマー材料フォーラム (ウインク愛知) , 2019.11, Poster (general), 高分子学会

• 有機無機ハイブリッドカプセル層を有する天然ナノ粒子の作製と機能化

  今西登志郎,福井有香,藤本啓二

  第28回ポリマー材料フォーラム (ウインク愛知) , 2019.11, Poster (general), 高分子学会

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• 骨疾患治療のための骨環境応答性ナノツールの開発

  2019.04

  -

  2021.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, 福井　有香, Grant-in-Aid for Early-Career Scientists , Principal Investigator

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• The Young Investigator Award Gold Prize(11th Liposome Research Days Conference)

  FUKUI Yuuka, 2008.07, Preparation of Lipo-nanocapsules by the layer-by-layer deposition of polysaccharides and coating of calcium phosphate onto a liposome

  Type of Award： Awards of National Conference, Council and Symposium

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• SEMINAR IN APPLIED CHEMISTRY

  2020

• LABORATORIES IN SCIENCE AND TECHNOLOGY

  2020

• LABORATORIES IN APPLIED CHEMISTRY D

  2020

• FRONTIER OF CHEMISTRY

  2020

• BACHELOR'S THESIS

  2020

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Memberships in Academic Societies 【 Display / hide 】

Memberships in Academic Societies 【 Display / hide 】

• Materials Research Society, 

  2011.11

  -

  Present

• American Chemical Society, 

  2009.05

  -

  Present

• 日本バイオマテリアル学会, 

  2007.11

  -

  Present

• 高分子学会, 

  2007.05

  -

  Present