蛭田　勇樹 (ヒルタ　ユウキ)

Hiruta, Yuki

写真a

所属（所属キャンパス）

理工学部 応用化学科 （矢上）

職名

准教授
このページの先頭へ▲

経歴 【 表示 ／ 非表示 】

  • 2010年04月
    -
    2011年03月

    慶應義塾大学大学院, 理工学研究科, 助教（有期・研究奨励）

  • 2011年04月
    -
    2013年03月

    日本学術振興会, 特別研究員（DC2）

  • 2013年04月
    -
    2017年03月

    慶應義塾大学, 薬学部, 助教

  • 2017年04月
    -
    2023年03月

    慶應義塾大学, 理工学部, 専任講師

  • 2023年04月
    -
    継続中

    慶應義塾大学, 理工学部, 准教授

このページの先頭へ▲

学歴 【 表示 ／ 非表示 】

  • 2004年04月
    -
    2008年03月

    慶應義塾大学, 理工学部, 応用化学科

    大学, 卒業

  • 2008年04月
    -
    2010年03月

    慶應義塾大学, 理工学研究科, 総合デザイン工学専攻

    大学院, 修了, 修士

  • 2010年04月
    -
    2013年03月

    慶應義塾大学, 理工学研究科, 総合デザイン工学専攻

    大学院, 修了, 博士

このページの先頭へ▲

学位 【 表示 ／ 非表示 】

  • 博士（工学）, 慶應義塾大学理工学研究科総合デザイン工学専攻, 論文

このページの先頭へ▲
 

著書 【 表示 ／ 非表示 】

  • Development of Near-Infrared Fluorescent Mg<sup>2+</sup> Probe and Application to Multicolor Imaging of Intracellular Signals

    Shindo Y., Ikeda Y., Hiruta Y., Citterio D., Oka K., Methods in Molecular Biology, 2021年

    　概要を見る

    Recent extensive studies revealed that the intracellular concentration of magnesium ions (Mg2+) is one of the important factors to regulate cellular functions. To evaluate the impact of Mg2+ concentration changes on intracellular signals or events, simultaneous imaging of Mg2+ with those phenomena is a powerful technique. The present protocol describes the synthesis and evaluation of near-infrared (NIR) fluorescent Mg2+-selective probes, named KMG-500 series, and the application to simultaneous imaging of the corresponding intracellular signal transductions and molecular events. The present protocol for multicolor imaging using fluorescent probes in the NIR and visible ranges is highly useful to reveal how multiple molecular events are correlated each other in each single cell.

このページの先頭へ▲

論文 【 表示 ／ 非表示 】

  • Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring

    Yoshii T., Nakano K., Okuda T., Citterio D., Hiruta Y.

    Analytical Methods （Analytical Methods)  15 （ 14 ） 1790 - 1796 2023年03月

    ISSN  17599660

    　概要を見る

    Eggshell-based reversed-phase packing materials were applied to an analytical column for high-performance liquid chromatography. Commercially available eggshell powder was classified by a cyclone system to obtain three types of particles with different diameters (arithmetic mean ± standard deviation: 4.3 ± 3.8, 5.6 ± 3.3, and 9.5 ± 5.5 μm). Sedimentation separation removed tiny particles from each sample, resulting in particles with arithmetic means of 6.6 ± 5.5, 7.3 ± 4.5, and 10.2 ± 5.0 μm, respectively. The unclassified particles and three particle types treated with sedimentation separation were subsequently packed into analytical columns (150 mm × 4.6 mm I.D.), and their separation efficiencies were evaluated by comparing their height equivalent to a theoretical plate (HETP). The column without sedimentation separation exhibited the highest HETP, whereas the columns with sedimentation separation showed better separation efficiency and lower back pressure. The column with the best separation efficiency was applied for the separation of 10 alkylbenzenes and 5 steroids, and all peaks were observed with complete separation (peak resolution: RS > 1.5). Finally, the column was used for quantitative analysis of voriconazole, an azole antifungal agent, and imatinib, a first-generation molecularly targeted drug for cancer treatment, in spiked whole blood. Excellent accuracy (99.1-102.8%) and precision (0.6-1.9%) were observed for the spiked drugs and long-term stability (>3000 column volumes of mobile phase flow) indicated good applicability of the developed eggshell-based column as an analytical column for routine analyses of therapeutic drugs in blood.

  • Evaluation of cellophane as platform for colorimetric assays on microfluidic analytical devices

    Shigemori Hiroki, Maejima Kento, Shibata Hiroyuki, Hiruta Yuki, Citterio Daniel

    MICROCHIMICA ACTA （Microchimica Acta)  190 （ 2 ）  2023年02月

    ISSN  0026-3672

    　概要を見る

    Due to their low cost, simplicity, and pump-free liquid transport properties, colorimetric assays on paper spots and microfluidic paper-based analytical devices (µPADs) are regarded as useful tools for point-of-care testing (POCT). However, for certain types of colorimetric assays, the “non-transparent” and “white” characters of paper can be a disadvantage. In this work, the possibilities of using cellophane as an alternative platform for colorimetric assays have been investigated. Cellophane is a low cost and easy-to-handle transparent film made of regenerated cellulose. Owing to its hydrophilic character, cellophane-based microfluidic channels fabricated through a print–cut–laminate approach enabled pump-free liquid transport into multiple detection areas, similar to µPADs. In addition, the water absorption characteristics of cellophane allowed the stable immobilization of water-soluble colorimetric indicators without any surface modification or additional reagents. The transparency of cellophane provides possibilities for simple background coloring of the substrates, increasing the dynamic signal range for hue-based colorimetric assays, as demonstrated for two model assays targeting H2O2 (46-fold increase) and creatinine (3.6-fold increase). Finally, a turbidity detection-based protein assay was realized on black background cellophane spots. The lowest limits of detection achieved with the cellophane-based devices were calculated as 7 µM for H2O2, 2.7 mg dL−1 for creatinine, and 3.5 mg dL−1 for protein (human serum albumin). Graphical Abstract: [Figure not available: see fulltext.]

  • Fabrication process development and basic evaluation of eggshell-based column packing material for reversed-phase preparative separation

    Yoshii Tomoka, Sakama Akihiro, Kanamori Kazuyoshi, Nakanishi Kazuki, Imai Hiroaki, Citterio Daniel, Hiruta Yuki

    JOURNAL OF CHROMATOGRAPHY A （Journal of Chromatography A)  1688 2023年01月

    ISSN  0021-9673

    　概要を見る

    Purification of basic drugs in reversed-phase mode is often difficult, mainly due to adsorption of positively charged compounds to the silica gel-based stationary phase. Since this adsorption can be suppressed under alkaline condition, columns with alkali-resistance are required. In addition, compounds with acid-sensitive structures are sometimes degraded during separation on silica gel-based columns which exhibit acidity due to their surface structure. We prepared an alkali-resistant reversed-phase packing material, Eggshell-PMAcO based on eggshells modified with an amphiphilic copolymer, poly(maleic acid-alt-1-octadecene) (PMAcO). The height equivalent to a theoretical plate (HETP) of the Eggshell-PMAcO column was improved by surface treatment with ammonium acetate buffer (900 mM, pH = 3.7), which is an inexpensive reagent, and the retention behavior for hydrophobic compounds was compared to a typical ODS column based on silica gel, resulting in sufficient selectivity of the eggshell-based column for hydrophobic compounds, as indicated by the ratio of retention factors of pentylbenzene and butylbenzene (Eggshell-PMAcO column: 1.55, ODS column: 1.65). Column temperature-dependent retention behavior of naphthalene was investigated in the temperature range from 25 °C to 45 °C, followed by the calculation of thermodynamic parameters. There was little difference in the standard molar enthalpy (Eggshell-PMAcO: −19.6 kJ/mol, ODS: −21.7 kJ/mol). The absolute value of the standard free Gibbs energy for the Eggshell-PMAcO column was much smaller than that of the ODS column (Eggshell-PMAcO: −0.284 kJ/mol, ODS: −13.0 kJ/mol), indicating that the Eggshell-PMAcO column had a weaker retention strength for naphthalene than the ODS column mainly due to the large difference in the standard molar entropy (Eggshell-PMAcO: −64.9 J/mol K, ODS column: −29.2 J/mol K). The retention capacities for imipramine under neutral (water/methanol) and alkaline (0.1% triethylamine water/methanol) conditions were 0.2 mg and 5 mg, respectively, based on injection mass-dependent HETP, retention factor and symmetry factor. Finally, the prepared column was applied to the purification of a building block for nucleic acid drugs. This study demonstrated that reversed-phase columns, which can be fabricated from eggshells and an amphiphilic copolymer in an inexpensive and eco-friendly way, have the ability to purify basic compounds and acid-sensitive compounds.

  • Effect of the side chain composition of mixed-charge polymers on pH-selective cell–membrane interactions

    Nakamura Y., Nasu M., Shindo Y., Oka K., Citterio D., Hiruta Y.

    Polymer Journal （Polymer Journal)  2023年

    ISSN  00323896

    　概要を見る

    Zwitterionic polymers are characterized by their antifouling ability due to their net-neutral charge and affinity for cancer cell surfaces. We developed six pH-responsive mixed-charge polymers and investigated the effect of their side chain hydrophobicity on their interaction with cancer cell membranes. The polymers were synthesized using [2-(methacryloyloxy)ethyl]trimethylammonium chloride, carboxylic acid monomers, and butyl methacrylate (BMA) as the neutral spacer moiety, and differences in hydrophobicity were created by altering the type of carboxylic acid monomer and ratio of the spacer. The surface charge of the polymers was designed to switch from net-neutral to positive in response to the weakly acidic tumor environment. This tuning occurred in a more cancer-like environment with the usage of a more hydrophobic anionic monomer and a higher ratio of BMA due to the weakening of electrostatic interactions. This trend was further confirmed by evaluation of the interaction between the polymers and heparin, an anionic polysaccharide that is used as a model for cancer cell surfaces. The affinity of the mixed-charge polymers for cells was then evaluated, and both the hydrophobicity and pH-responsive nature of these polymers led to pH-selective toxicity, which was assumed to be caused by the disruption of cell membrane integrity.

  • Amphiphilic Copolymer-Modified Eggshell-Based Column Packing Materials for the Preparative Separation of Basic Drugs

    Yoshii T., Mochida M., Kaizu K., Soda Y., Kanamori K., Nakanishi K., Sato T., Imai H., Citterio D., Hiruta Y.

    ACS Applied Polymer Materials （ACS Applied Polymer Materials)  4 （ 10 ） 6949 - 6957 2022年10月

    ISSN  2637-6105

    　概要を見る

    A way to utilize eggshells is desired because most of the eggshell waste is disposed of in landfills. Despite eggshells having attractive features such as a thermodynamically stable porous crystalline structure, their effective utilization as a functional material has yet to be demonstrated. Herein, we developed an alkali-stable column packing material for reversed-phase preparative high-performance liquid chromatography (HPLC) based on eggshells. Nanocrystals and biopolymers present on eggshell powder were removed by surface treatment with buffered ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite solutions, resulting in bare calcium carbonate with a uniform porous surface. The obtained particles were modified with poly(maleic acid-alt-1-octadecene) (PMAcO) having hydrophobic alkyl chains and anionic carboxylate groups. The prepared PMAcO-modified eggshell-based material was applied to an HPLC column, and its performance is evaluated. The column succeeded in the separation of alkylbenzenes, steroids, and diastereomers. It was also used for the separation of basic tricyclic antidepressants in an alkaline mobile phase, showing excellent retention capacity and alkali stability. This study demonstrates the potential of eggshell utilization, providing not only a practical stationary phase for HPLC but also a solution for recycling calcium carbonate-based biomineral waste.

全件表示 >>

このページの先頭へ▲

KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

このページの先頭へ▲

研究発表 【 表示 ／ 非表示 】

  • Eggshell-based Packing Materials for HPLC

    Yuki Hiruta

    HPLC 2022, 

    2022年06月
    , 

    ポスター発表

  • Development of Furimazine Derivatives for Long-Term Bioluminescence Imaging

    蛭田勇樹

    日本顕微鏡学会　第78回学術講演会, 

    2022年05月
    , 

    口頭発表（招待・特別）

  • 炭酸カルシウムを母体とするHPLC用充填剤の開発

    蛭田勇樹

    日本化学会第101春季年会, 

    2022年03月
    , 

    ポスター発表

  • Development of caged luciferin enabling spatiotemporal trans-scale imaging

    Yuki Hiruta

    Pacifichem 2021, 

    2021年12月
    -
    2121年12月
    , 

    口頭発表（一般）

  • Development of stimuli-responsive polymers for diagnostic and therapeutic applications

    Yuki Hiruta

    2021 MRS-T International Conference, 

    2021年11月
    , 

    口頭発表（招待・特別）

全件表示 >>

このページの先頭へ▲

競争的研究費の研究課題 【 表示 ／ 非表示 】

  • がんバイオマーカー応答性高分子設計を戦略とした光セラノスティクス薬剤の開発

    2021年04月
    -
    2024年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 蛭田 勇樹, 基盤研究(C), 補助金,  研究代表者

  • 時空間トランススケールイメージングを可能にする超分子ケージドルシフェリンの開発

    2021年04月
    -
    2023年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 蛭田 勇樹, 新学術領域研究（研究領域提案型）, 補助金,  研究代表者

  • 時空間トランススケールイメージングを可能にする高分子ケージドルシフェリンの開発

    2019年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 蛭田　勇樹, 新学術領域研究（研究課題提案型）, 補助金,  研究代表者

  • 腫瘍への自己集積増幅機能を持つセラノスティクスナノプラットフォームの創製

    2019年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 蛭田　勇樹, 若手研究, 補助金,  研究代表者

  • 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

    2018年04月
    -
    2021年03月

    チッテリオ ダニエル, 補助金,  研究分担者

全件表示 >>

このページの先頭へ▲

受賞 【 表示 ／ 非表示 】

  • Best Poster Award HPLC 2022, San Diego

    2022年06月

    受賞区分： 国際学会・会議・シンポジウム等の賞

  • Outstanding Reviewer for journal of Materials Chemistry B

    2021年03月

    受賞区分： 出版社・新聞社・財団等の賞

  • 日本分析化学会奨励賞

    2020年08月, 公益社団法人日本分析化学会, 精密分子設計に基づくバイオイメージングプローブの開発と応用

    受賞区分： 国内学会・会議・シンポジウム等の賞

  • 高分子研究奨励賞

    Development of stimuli-responsive polymer materials for application to medical analysis, 2020年05月, 公益社団法人高分子学会, 医療分析への応用を志向した刺激応答性高分子材料の開発

    受賞区分： 国内学会・会議・シンポジウム等の賞

  • 日本化学会第９９春季年会 優秀講演賞（学術）

    2019年04月, 公益財団法人 日本化学会

    受賞区分： 国内学会・会議・シンポジウム等の賞

全件表示 >>

このページの先頭へ▲
 

担当授業科目 【 表示 ／ 非表示 】

  • 応用化学輪講

    2023年度

  • 機器分析総論

    2023年度

  • ナノスケール科学ジョイントセミナー

    2023年度

  • マテリアルデザイン科学ジョイントセミナー

    2023年度

  • 自然科学実験

    2023年度

全件表示 >>

このページの先頭へ▲
 

所属学協会 【 表示 ／ 非表示 】

  • クロマトグラフィー科学会会員, 

    2016年01月
    -
    継続中

  • 高分子学会会員, 

    2016年01月
    -
    継続中

  • 日本DDS学会会員, 

    2014年04月
    -
    継続中

  • 日本薬学会会員, 

    2014年01月
    -
    継続中

  • 日本化学会会員, 

    2010年01月
    -
    継続中

全件表示 >>

このページの先頭へ▲

委員歴 【 表示 ／ 非表示 】

  • 2021年01月
    -
    継続中

    関東支部常任幹事 , 日本分析化学会

  • 2019年12月
    -
    継続中

    化学グランプリ・オリンピック委員会 グランプリ小委員会 委員, 公益社団法人日本化学会

  • 2018年04月
    -
    継続中

    代議員, 日本分析化学会

  • 2017年07月
    -
    継続中

    幹事, 日本分析化学会関東支部若手の会

  • 2017年01月
    -
    2018年12月

    関東支部幹事, 日本分析化学会

このページの先頭へ▲