Details of a Researcher

Hiruta, Yuki

写真a

Affiliation: Faculty of Science and Technology, Department of Applied Chemistry （Yagami）

Position: Associate Professor

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Career 【 Display / hide 】

2010.04

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2011.03

Keio University, Graduate School of Science and Technology, Assistant Professor
2011.04

-

2013.03

Japan Society for the Promition of Science, Research Fellowship for Young Scientists (DC2)
2013.04

-

2017.03

Keio University, Faculty of Pharmacy, Assistant Professor
2017.04

-

2023.03

Keio University, Faculty of Science and Technology, Senior Assistant Professor
2023.04

-

Present

Keio University, Faculty of Science and Technology, Associate Professor

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Academic Background 【 Display / hide 】

2004.04

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2008.03

Keio University, Faculty of Science and Technology, Department of Applied Chemistry

University, Graduated
2008.04

-

2010.03

Keio University, Graduate School of Science and Technology, School of Integrated Design Engineering

Graduate School, Completed, Master's course
2010.04

-

2013.03

Keio University, Graduate School of Science and Technology, School of Integrated Design Engineering

Graduate School, Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

博士（工学）, 慶應義塾大学理工学研究科総合デザイン工学専攻, Dissertation

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Books 【 Display / hide 】

Development of Near-Infrared Fluorescent Mg2+ Probe and Application to Multicolor Imaging of Intracellular Signals

Shindo Y., Ikeda Y., Hiruta Y., Citterio D., Oka K., Methods in Molecular Biology, 2021

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Recent extensive studies revealed that the intracellular concentration of magnesium ions (Mg2+) is one of the important factors to regulate cellular functions. To evaluate the impact of Mg2+ concentration changes on intracellular signals or events, simultaneous imaging of Mg2+ with those phenomena is a powerful technique. The present protocol describes the synthesis and evaluation of near-infrared (NIR) fluorescent Mg2+-selective probes, named KMG-500 series, and the application to simultaneous imaging of the corresponding intracellular signal transductions and molecular events. The present protocol for multicolor imaging using fluorescent probes in the NIR and visible ranges is highly useful to reveal how multiple molecular events are correlated each other in each single cell.

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Papers 【 Display / hide 】

Rational design of pH-responsive near-infrared spirocyclic cyanines: the effects of substituents and the external environment

Sakama, A; Seo, H; Hara, J; Shindo, Y; Ikeda, Y; Oka, K; Citterio, D; Hiruta, Y

CHEMICAL COMMUNICATIONS 60 （ 46 ） 5984 - 5987 2024.06

ISSN 1359-7345
- Access to Document (DOI)
Simplified capture, extraction, and amplification of cellular DNA from water samples

Kawaguchi M., Aoki H., Kamo H., Miura K., Hiruta Y., Simizu S., Citterio D.

Analytical Sciences （Analytical Sciences) 40 （ 3 ） 501 - 510 2024.03

ISSN 09106340

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DNA analysis in water samples is attracting attention in various fields. However, conventional methods for DNA analysis require a work-intensive and time-consuming sample pre-treatment. In this study, a simplified pre-treatment method for analyzing DNA in water samples was evaluated. The process consists of filtration, DNA extraction, and amplification, which can be achieved within a short time. In the filtration process, two types of filters, firstly a tissue paper (Kimwipe) and then a glass filter (GF/F), were used in sequence. The first large pore size filter enabled a reduction in filtration time by removing large particulate matter impurities present in river water matrix. Cells spiked into 1 L of river water were recovered at more than 90% within approximately 5 min filtration time. Also, DNA was extracted from the captured cells directly on the surface of the filter in only 5 min. Thus, DNA collection and extraction from a water sample can be completed within about 10 min. Furthermore, PCR amplification was performed directly from DNA-attached filter sections, which greatly reduced the number of required pre-treatment steps. Finally, we succeeded in establishing a simple and fast on-site pre-treatment system by using a hand-driven syringe filtration method. This pre-treatment system is expected to offer the possibility for the future establishment of a rapid and easy DNA analysis method applicable to various types of water samples. Graphical abstract: (Figure presented.)
- Access to Document (DOI)
Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring (vol 15, pg 1790, 2023)

Yoshii, T; Nakano, K; Okuda, T; Citterio, D; Hiruta, Y

ANALYTICAL METHODS 16 （ 9 ） 1416 - 1416 2024.02

ISSN 1759-9660
- Access to Document (DOI)
Development of Phosphinate Ligand-Based Low-Affinity Ca2+ Fluorescent Probes and Application to Intracellular Ca2+ Imaging

Kumada R., Sakama A., Shindo Y., Kuronuma Y., Iwasawa N., Citterio D., Oka K., Hiruta Y.

Analytical Chemistry （Analytical Chemistry) 95 （ 45 ） 16683 - 16691 2023.11

ISSN 00032700

　View Summary

Divalent metal cations such as calcium ion (Ca2+) and magnesium ion (Mg2+) are indispensable to the regulation of various cellular activities. In this research, we developed the KLCA series utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a target binding site, which was reported recently as a highly free Mg2+-selective ligand. KLCA-301 with orange fluorescence based on a rhodamine fluorophore and KLCA-501 with near-infrared (NIR) fluorescence based on a Si-rhodamine fluorophore were synthesized, intended for application to multicolor imaging. The evaluation of the fluorescence response to Ca2+ and Mg2+ of the KLCA series indicated the applicability as low-affinity Ca2+ probes. While KLCA-301 mainly localized in the cytosol in cultured rat hippocampal neurons, KLCA-501 localized to the cytosol and granular organelles in neurons. Comparison of the fluorescence response of KLCA-301 and the high-affinity Ca2+ probe Fluo-4 upon stimulation by glutamate in stained neurons revealed that KLCA-301 could reflect the secondary large rise of intracellular Ca2+, which Fluo-4 could not detect. In addition, KLCA-501 showed a fluorescence response similar to the low-affinity Ca2+ probe Fluo-5N upon stimulation by glutamate in stained neurons, concluding that KLCA-301 and KLCA-501 could be used as low-affinity Ca2+ probes. The KLCA series offers new options for low-affinity Ca2+ probes. Moreover, KLCA-501 achieved simultaneous visualization of the change in Ca2+ and ATP concentrations and also in mitochondrial inner membrane potential in neurons. KLCA-501 is expected to be a strong tool that enables simultaneous multicolor imaging of multiple targets and elucidation of their relationship in cells.
- Access to Document (DOI)
Cation/anion-exchange mode switching chromatography utilizing pH-responsive mixed charge polymer-modified silica beads

Kaku T., Deura K., Yoshii T., Citterio D., Hiruta Y.

Molecular Systems Design and Engineering （Molecular Systems Design and Engineering) 9 （ 1 ） 56 - 62 2023.11

ISSN 2058-9689

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The separation capacity of a column typically remains constant. By applying stimuli-responsive materials to the stationary phase, the separation capacity in a single column can be tuned; however, the separation mode is not completely switched. In this study, we aimed to develop a cation/anion-exchange mode switching chromatography approach, in which the monomer ratio is adjusted, enabling the surface charge to become either negative or positive in response to mobile phase pH. Three types of beads were prepared, each modified with a pH-responsive mixed-charge polymer combining a cationic monomer, a pH-responsive carboxylic acid monomer, a neutral monomer, and a cross-linking monomer. The composition ratio of the cationic monomer to the pH-responsive carboxylic acid monomer was set at 1 : 2 so that the cation-exchange mode occurs at a pH above the pKa and the anion-exchange mode occurs below the pKa. At a pH below the pKa, the retention factor of the negatively charged compound increased. In contrast, at a pH above the pKa, the retention factor of the positively charged compound increased, confirming the charge switching on the bead surface. Switching to the cation- and anion-exchange mode enabled the separation of five basic antidepressants and acidic non-steroidal anti-inflammatory drugs, respectively. Utilizing a pH-responsive mixed-charge polymer, we attributed a cation/anion-exchange mode to a single column.
- Access to Document (DOI)

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Papers, etc., Registered in KOARA 【 Display / hide 】

Optical imaging technology for manipulating and observing intracellular magnesium ions

Hiruta, Yūki

福澤諭吉記念慶應義塾学事振興基金事業報告集（福澤基金運営委員会) 2020
Development of dual pH-responsive drug delivery career by the logical design of polymer

Hiruta, Yuki

科学研究費補助金研究成果報告書 2016
The development of environmentally responsive fluorescence polymer probes for visualizing pathological cells

Hiruta, Yuki

科学研究費補助金研究成果報告書 2014

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Presentations 【 Display / hide 】

Eggshell-based Packing Materials for HPLC

Yuki Hiruta

HPLC 2022,

2022.06
,
Poster presentation
Development of Furimazine Derivatives for Long-Term Bioluminescence Imaging

蛭田勇樹

日本顕微鏡学会　第78回学術講演会,

2022.05
,
Oral presentation (invited, special)
炭酸カルシウムを母体とするHPLC用充填剤の開発

蛭田勇樹

日本化学会第101春季年会,

2022.03
,
Poster presentation
Development of caged luciferin enabling spatiotemporal trans-scale imaging

Yuki Hiruta

Pacifichem 2021,

2021.12

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2121.12
,
Oral presentation (general)
Development of stimuli-responsive polymers for diagnostic and therapeutic applications

Yuki Hiruta

2021 MRS-T International Conference,

2021.11
,
Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

がんバイオマーカー応答性高分子を基盤とした光セラノスティクスプラットフォーム構築

2023.04

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2026.03

文部科学省, 日本学術振興会科学研究費助成事業国際共同研究加速基金(国際共同研究強化(A)), Principal investigator
生命科学研究を拓く生物発光技術の開発

2023.04

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2024.03

文部科学省, 日本学術振興会科学研究費助成事業基盤研究(B), Coinvestigator(s)
がんバイオマーカー応答性高分子設計を戦略とした光セラノスティクス薬剤の開発

2021.04

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2024.03

MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator
時空間トランススケールイメージングを可能にする超分子ケージドルシフェリンの開発

2021.04

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2023.03

MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas, Principal investigator
Development of polymer caged luciferin enabling spatiotemporal trans-scale imaging

2019.04

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2021.03

MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas, Principal investigator

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Awards 【 Display / hide 】

Best Poster Award HPLC 2022, San Diego

2022.06

Type of Award： Award from international society, conference, symposium, etc.
Outstanding Reviewer for journal of Materials Chemistry B

2021.03

Type of Award： Award from publisher, newspaper, foundation, etc.
Award for Encouragement of Research in Analytical Chemistry

2020.08, The Japan Society of Analytical Chemistry, Development and application of bioimaging probes based on precision molecular design

Type of Award： Award from Japanese society, conference, symposium, etc.
Award for Encouragement of Research in Polymer Science

Development of stimuli-responsive polymer materials for application to medical analysis, 2020.05, 公益社団法人高分子学会, 医療分析への応用を志向した刺激応答性高分子材料の開発

Type of Award： Award from Japanese society, conference, symposium, etc.
日本化学会第９９春季年会優秀講演賞（学術）

2019.04, 公益財団法人日本化学会

Type of Award： Award from Japanese society, conference, symposium, etc.