Hiruta, Yuki

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry (Yagami)

Position

Associate Professor
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Career 【 Display / hide 】

  • 2010.04
    -
    2011.03

    Keio University, Graduate School of Science and Technology, Assistant Professor

  • 2011.04
    -
    2013.03

    Japan Society for the Promition of Science, Research Fellowship for Young Scientists (DC2)

  • 2013.04
    -
    2017.03

    Keio University, Faculty of Pharmacy, Assistant Professor

  • 2017.04
    -
    2023.03

    Keio University, Faculty of Science and Technology, Senior Assistant Professor

  • 2023.04
    -
    Present

    Keio University, Faculty of Science and Technology, Associate Professor

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Academic Background 【 Display / hide 】

  • 2004.04
    -
    2008.03

    Keio University, Faculty of Science and Technology, Department of Applied Chemistry

    University, Graduated

  • 2008.04
    -
    2010.03

    Keio University, Graduate School of Science and Technology, School of Integrated Design Engineering

    Graduate School, Completed, Master's course

  • 2010.04
    -
    2013.03

    Keio University, Graduate School of Science and Technology, School of Integrated Design Engineering

    Graduate School, Completed, Doctoral course

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Academic Degrees 【 Display / hide 】

  • 博士（工学）, 慶應義塾大学理工学研究科総合デザイン工学専攻, Dissertation

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Books 【 Display / hide 】

  • Development of Near-Infrared Fluorescent Mg<sup>2+</sup> Probe and Application to Multicolor Imaging of Intracellular Signals

    Shindo Y., Ikeda Y., Hiruta Y., Citterio D., Oka K., Methods in Molecular Biology, 2021

    　View Summary

    Recent extensive studies revealed that the intracellular concentration of magnesium ions (Mg2+) is one of the important factors to regulate cellular functions. To evaluate the impact of Mg2+ concentration changes on intracellular signals or events, simultaneous imaging of Mg2+ with those phenomena is a powerful technique. The present protocol describes the synthesis and evaluation of near-infrared (NIR) fluorescent Mg2+-selective probes, named KMG-500 series, and the application to simultaneous imaging of the corresponding intracellular signal transductions and molecular events. The present protocol for multicolor imaging using fluorescent probes in the NIR and visible ranges is highly useful to reveal how multiple molecular events are correlated each other in each single cell.

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Papers 【 Display / hide 】

  • Origami Paper-Based Immunoassay Device with CRISPR/Cas12a Signal Amplification

    Suzuki, H; Tong, GD; Nath, P; Hiruta, Y; Citterio, D

    ACS SENSORS  2025.03

    ISSN  2379-3694

  • The latest developments of near-infrared fluorescent probes from NIR-I to NIR-II for bioimaging

    Kuronuma, Y; Watanabe, R; Hiruta, Y

    ANALYTICAL SCIENCES  2025.02

    ISSN  0910-6340

  • Ratiometric Imaging for Quantification of Elevated Ca<sup>2+</sup> in Neurons Using Synthetic Low-Affinity Fluorescent Probe

    Kuronuma Y., Shindo Y., Kumada R., Sakama A., Citterio D., Oka K., Hiruta Y.

    ACS Chemical Neuroscience 16 ( 4 ) 649 - 658 2025.02

    Research paper (scientific journal), Last author, Corresponding author, Accepted,  ISSN  1948-7193

    　View Summary

    The availability of various calcium ion (Ca2+) fluorescent probes has contributed to revealing physiological events related to intracellular Ca2+. However, conventional probes face challenges for quantitatively and selectively visualizing high Ca2+ concentrations in cells induced by any stimuli, including biomolecules or electrical signal that disrupt Ca2+ homeostasis. In this report, we designed and synthesized a low-affinity ratiometric Ca2+ probe, KLCA-Fura, utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a ligand, for which we recently demonstrated the suitability as a new low-affinity ligand for Ca2+. KLCA-Fura showed a blue shift in excitation wavelength with increasing Ca2+ concentration based on the intramolecular charge transfer (ICT). Its affinity for Ca2+ is lower than commercially available conventional Ca2+ probes. Furthermore, the selectivity for Ca2+ and the fluorescence intensity were considered sufficient to accurately detect Ca2+. The corresponding acetoxymethyl ester, KLCA-FuraAM, was synthesized for intracellular imaging and applied to Ca2+ quantification in neurons. KLCA-FuraAM enabled quantitative ratiometric monitoring of the two-step Ca2+ concentration increase induced by glutamate stimulation. While this two-step response was not clearly observed with a commercially available low-affinity ratiometric Ca2+ probe, Fura-FF, KLCA-FuraAM has demonstrated the potential to quantitatively visualize the behavior of high Ca2+ concentrations. The ratiometric low-affinity Ca2+ probe, KLCA-Fura, is expected to be a powerful tool for discovering new functions of Ca2+ in neurons.

  • Creating coveted bioluminescence colors for simultaneous multi-color bioimaging

    Hattori, M; Wazawa, T; Orioka, M; Hiruta, Y; Nagai, T

    Science Advances 11 ( 4 ) eadp4750 2025.01

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  2375-2548

  • 3D-printed devices for multiplexed semi-quantitative competitive lateral flow immunoassays

    Tong, GD; Misawa, K; Jarujamrus, P; Hiruta, Y; Citterio, D

    ANALYTICAL METHODS 17 ( 6 ) 1206 - 1213 2025.01

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  1759-9660

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Presentations 【 Display / hide 】

  • Development of column packing materials using functional polymers and application to pharmaceutical analysis

    The 35th Conference of Chromatographic Sciences, 

    2024.11
    , 

    Oral presentation (invited, special)

  • ペプチドミメティック分子設計によるpH応答性高分子の開発

    蛭田 勇樹

    第73回高分子討論会, 

    2024.09
    , 

    Oral presentation (general)

  • スピロ環化平衡を利用したpH応答性近赤外蛍光シアニンプローブ

    蛭田 勇樹

    日本分析化学会第73年会, 

    2024.09
    , 

    Oral presentation (general)

  • Cation/anion-exchange mode switching chromatography utilizing pH-responsive polymer for pharmaceutical separations

    Yuki Hiruta

    HPLC 2024, 

    2024.07
    , 

    Poster presentation

  • Evaluation of hydrophilic brush copolymers on biodistribution for tumor-targeted carriers of diagnostic drugs

    Yuki Hiruta

    第40回日本DDS学会学術集会, 

    2024.07
    , 

    Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • がんバイオマーカー応答性高分子を基盤とした光セラノスティクスプラットフォーム構築

    2023.04
    -
    2026.03

    文部科学省, 日本学術振興会 科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(A)), Principal investigator

  • 生命科学研究を拓く生物発光技術の開発

    2023.04
    -
    2024.03

    文部科学省, 日本学術振興会 科学研究費助成事業 基盤研究(B), Coinvestigator(s)

  • がんバイオマーカー応答性高分子設計を戦略とした光セラノスティクス薬剤の開発

    2021.04
    -
    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

  • 時空間トランススケールイメージングを可能にする超分子ケージドルシフェリンの開発

    2021.04
    -
    2023.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas, Principal investigator

  • Development of polymer caged luciferin enabling spatiotemporal trans-scale imaging

    2019.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas, Principal investigator

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Awards 【 Display / hide 】

  • Award for Encouragement of Research in Chromatographic Sciences

    2024.11, The Society for Chromatographic Sciences, Development of column packing materials using functional polymers and application to pharmaceutical analysis

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • Best Poster Award HPLC 2022, San Diego

    2022.06

    Type of Award： Award from international society, conference, symposium, etc.

  • Outstanding Reviewer for journal of Materials Chemistry B

    2021.03

    Type of Award： Award from publisher, newspaper, foundation, etc.

  • Award for Encouragement of Research in Analytical Chemistry

    2020.08, The Japan Society of Analytical Chemistry, Development and application of bioimaging probes based on precision molecular design

    Type of Award： Award from Japanese society, conference, symposium, etc.

  • Award for Encouragement of Research in Polymer Science

    Development of stimuli-responsive polymer materials for application to medical analysis, 2020.05, 公益社団法人高分子学会, 医療分析への応用を志向した刺激応答性高分子材料の開発

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • SEMINAR IN APPLIED CHEMISTRY

    2025

  • PRACTICAL INSTRUMENTAL ANALYSIS

    2025

  • NANO SCALE SCIENCE JOINT SEMINAR

    2025

  • MATERIAL DESIGN SCIENCE JOINT SEMINAR

    2025

  • LABORATORY IN SCIENCE

    2025

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Memberships in Academic Societies 【 Display / hide 】

  • クロマトグラフィー科学会会員, 

    2016.01
    -
    Present

  • 高分子学会会員, 

    2016.01
    -
    Present

  • 日本DDS学会会員, 

    2014.04
    -
    Present

  • 日本薬学会会員, 

    2014.01
    -
    Present

  • 日本化学会会員, 

    2010.01
    -
    Present

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Committee Experiences 【 Display / hide 】

  • 2024.11
    -
    Present

    生物発光化学発光研究会世話人, 生物発光化学発光研究会

  • 2024.03
    -
    Present

    Associate Editor in Analytical Sciences, The Japan Society for Analytical Chemistry

  • 2021.01
    -
    2022.12

    関東支部常任幹事 , 日本分析化学会

  • 2019.12
    -
    2021.11

    化学グランプリ・オリンピック委員会 グランプリ小委員会 委員, 公益社団法人日本化学会

  • 2018.04
    -
    Present

    代議員, 日本分析化学会

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