Hiruta, Yuki

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry (Yagami)

Position

Associate Professor

Career 【 Display / hide

  • 2010.04
    -
    2011.03

    Keio University, Graduate School of Science and Technology, Assistant Professor

  • 2011.04
    -
    2013.03

    Japan Society for the Promition of Science, Research Fellowship for Young Scientists (DC2)

  • 2013.04
    -
    2017.03

    Keio University, Faculty of Pharmacy, Assistant Professor

  • 2017.04
    -
    2023.03

    Keio University, Faculty of Science and Technology, Senior Assistant Professor

  • 2023.04
    -
    Present

    Keio University, Faculty of Science and Technology, Associate Professor

Academic Background 【 Display / hide

  • 2004.04
    -
    2008.03

    Keio University, Faculty of Science and Technology, Department of Applied Chemistry

    University, Graduated

  • 2008.04
    -
    2010.03

    Keio University, Graduate School of Science and Technology, School of Integrated Design Engineering

    Graduate School, Completed, Master's course

  • 2010.04
    -
    2013.03

    Keio University, Graduate School of Science and Technology, School of Integrated Design Engineering

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(工学), 慶應義塾大学理工学研究科総合デザイン工学専攻, Dissertation

 

Books 【 Display / hide

  • Development of Near-Infrared Fluorescent Mg<sup>2+</sup> Probe and Application to Multicolor Imaging of Intracellular Signals

    Shindo Y., Ikeda Y., Hiruta Y., Citterio D., Oka K., Methods in Molecular Biology, 2021

     View Summary

    Recent extensive studies revealed that the intracellular concentration of magnesium ions (Mg2+) is one of the important factors to regulate cellular functions. To evaluate the impact of Mg2+ concentration changes on intracellular signals or events, simultaneous imaging of Mg2+ with those phenomena is a powerful technique. The present protocol describes the synthesis and evaluation of near-infrared (NIR) fluorescent Mg2+-selective probes, named KMG-500 series, and the application to simultaneous imaging of the corresponding intracellular signal transductions and molecular events. The present protocol for multicolor imaging using fluorescent probes in the NIR and visible ranges is highly useful to reveal how multiple molecular events are correlated each other in each single cell.

Papers 【 Display / hide

  • Semi-quantitative microfluidic paper-based analytical device for ionic silica detection

    Ogawa M., Katoh A., Matsubara R., Kondo H., Otsuka M., Sawatsubashi T., Hiruta Y., Citterio D.

    Analytical Sciences (Analytical Sciences)  39 ( 8 ) 1361 - 1370 2023.08

    ISSN  09106340

     View Summary

    Silicate ions (SiO32−), or ionic silica, are known to cause silica scaling in industrial water applications when excess amounts are present; hence, concentrations must be monitored and kept at a constant low level. Ionic silica is conventionally measured by spectrophotometry in the form of its silicomolybdic complex based on the molybdenum blue reaction, but the operation process is complicated and not suitable for on-site detection. To solve these issues, microfluidic paper-based analytical devices (µPADs) have been gaining attention as portable, low-cost analytical devices suitable for on-site detection. The foldable origami type device described in this work enabled silica detection based on the molybdenum blue reaction, in the range of 50–1000 mg/L, with a practically detectable lowest concentration of 50 mg/L. The device showed selectivity for silicate ions and stability over 21 days when stored at 4 °C. The semi-quantitative analytical performance makes the proposed paper-based device attractive for on-site industrial monitoring. Graphical abstract: [Figure not available: see fulltext.]

  • Development of prediction model for cloud point of thermo-responsive polymers by experiment-oriented materials informatics

    Hayakawa M., Sakano K., Kumada R., Tobita H., Igarashi Y., Citterio D., Oaki Y., Hiruta Y.

    Polymer Chemistry (Polymer Chemistry)  14 ( 19 ) 2383 - 2389 2023.04

    ISSN  17599954

     View Summary

    Thermo-responsive polymers having a lower critical solution temperature (LCST) have attracted attention for biological applications such as drug delivery, diagnosis, and coating materials. In recent years, research on predicting LCST by utilizing machine learning has been conducted. However, since these methods targeted only copolymers combining specific monomer structures, they are not versatile, and multiple trials are still required to obtain new thermo-responsive polymers with the desired LCST. In this study, a prediction model for cloud point temperature (TCP) was built by a combination of materials informatics and chemical insight, named sparse modeling for small data (SpM-S) using a small dataset of polymers collected from the literature as training data. This approach created a model that is interpretable, easy to calculate, and versatile. The prediction accuracy was validated using data from different literature sources and experimental test data. The model was able to predict the TCP of polymers containing monomers not included in the dataset as well as polymers containing monomers included in the dataset. The predictive model has the potential to guide the design of new thermo-responsive polymers, and to contribute to efficient development of thermo-responsive polymers.

  • Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring

    Yoshii T., Nakano K., Okuda T., Citterio D., Hiruta Y.

    Analytical Methods (Analytical Methods)  15 ( 14 ) 1790 - 1796 2023.03

    ISSN  17599660

     View Summary

    Eggshell-based reversed-phase packing materials were applied to an analytical column for high-performance liquid chromatography. Commercially available eggshell powder was classified by a cyclone system to obtain three types of particles with different diameters (arithmetic mean ± standard deviation: 4.3 ± 3.8, 5.6 ± 3.3, and 9.5 ± 5.5 μm). Sedimentation separation removed tiny particles from each sample, resulting in particles with arithmetic means of 6.6 ± 5.5, 7.3 ± 4.5, and 10.2 ± 5.0 μm, respectively. The unclassified particles and three particle types treated with sedimentation separation were subsequently packed into analytical columns (150 mm × 4.6 mm I.D.), and their separation efficiencies were evaluated by comparing their height equivalent to a theoretical plate (HETP). The column without sedimentation separation exhibited the highest HETP, whereas the columns with sedimentation separation showed better separation efficiency and lower back pressure. The column with the best separation efficiency was applied for the separation of 10 alkylbenzenes and 5 steroids, and all peaks were observed with complete separation (peak resolution: RS > 1.5). Finally, the column was used for quantitative analysis of voriconazole, an azole antifungal agent, and imatinib, a first-generation molecularly targeted drug for cancer treatment, in spiked whole blood. Excellent accuracy (99.1-102.8%) and precision (0.6-1.9%) were observed for the spiked drugs and long-term stability (>3000 column volumes of mobile phase flow) indicated good applicability of the developed eggshell-based column as an analytical column for routine analyses of therapeutic drugs in blood.

  • Evaluation of cellophane as platform for colorimetric assays on microfluidic analytical devices

    Shigemori Hiroki, Maejima Kento, Shibata Hiroyuki, Hiruta Yuki, Citterio Daniel

    MICROCHIMICA ACTA (Microchimica Acta)  190 ( 2 )  2023.02

    ISSN  0026-3672

     View Summary

    Due to their low cost, simplicity, and pump-free liquid transport properties, colorimetric assays on paper spots and microfluidic paper-based analytical devices (µPADs) are regarded as useful tools for point-of-care testing (POCT). However, for certain types of colorimetric assays, the “non-transparent” and “white” characters of paper can be a disadvantage. In this work, the possibilities of using cellophane as an alternative platform for colorimetric assays have been investigated. Cellophane is a low cost and easy-to-handle transparent film made of regenerated cellulose. Owing to its hydrophilic character, cellophane-based microfluidic channels fabricated through a print–cut–laminate approach enabled pump-free liquid transport into multiple detection areas, similar to µPADs. In addition, the water absorption characteristics of cellophane allowed the stable immobilization of water-soluble colorimetric indicators without any surface modification or additional reagents. The transparency of cellophane provides possibilities for simple background coloring of the substrates, increasing the dynamic signal range for hue-based colorimetric assays, as demonstrated for two model assays targeting H2O2 (46-fold increase) and creatinine (3.6-fold increase). Finally, a turbidity detection-based protein assay was realized on black background cellophane spots. The lowest limits of detection achieved with the cellophane-based devices were calculated as 7 µM for H2O2, 2.7 mg dL−1 for creatinine, and 3.5 mg dL−1 for protein (human serum albumin). Graphical Abstract: [Figure not available: see fulltext.]

  • Fabrication process development and basic evaluation of eggshell-based column packing material for reversed-phase preparative separation

    Yoshii Tomoka, Sakama Akihiro, Kanamori Kazuyoshi, Nakanishi Kazuki, Imai Hiroaki, Citterio Daniel, Hiruta Yuki

    JOURNAL OF CHROMATOGRAPHY A (Journal of Chromatography A)  1688 2023.01

    ISSN  0021-9673

     View Summary

    Purification of basic drugs in reversed-phase mode is often difficult, mainly due to adsorption of positively charged compounds to the silica gel-based stationary phase. Since this adsorption can be suppressed under alkaline condition, columns with alkali-resistance are required. In addition, compounds with acid-sensitive structures are sometimes degraded during separation on silica gel-based columns which exhibit acidity due to their surface structure. We prepared an alkali-resistant reversed-phase packing material, Eggshell-PMAcO based on eggshells modified with an amphiphilic copolymer, poly(maleic acid-alt-1-octadecene) (PMAcO). The height equivalent to a theoretical plate (HETP) of the Eggshell-PMAcO column was improved by surface treatment with ammonium acetate buffer (900 mM, pH = 3.7), which is an inexpensive reagent, and the retention behavior for hydrophobic compounds was compared to a typical ODS column based on silica gel, resulting in sufficient selectivity of the eggshell-based column for hydrophobic compounds, as indicated by the ratio of retention factors of pentylbenzene and butylbenzene (Eggshell-PMAcO column: 1.55, ODS column: 1.65). Column temperature-dependent retention behavior of naphthalene was investigated in the temperature range from 25 °C to 45 °C, followed by the calculation of thermodynamic parameters. There was little difference in the standard molar enthalpy (Eggshell-PMAcO: −19.6 kJ/mol, ODS: −21.7 kJ/mol). The absolute value of the standard free Gibbs energy for the Eggshell-PMAcO column was much smaller than that of the ODS column (Eggshell-PMAcO: −0.284 kJ/mol, ODS: −13.0 kJ/mol), indicating that the Eggshell-PMAcO column had a weaker retention strength for naphthalene than the ODS column mainly due to the large difference in the standard molar entropy (Eggshell-PMAcO: −64.9 J/mol K, ODS column: −29.2 J/mol K). The retention capacities for imipramine under neutral (water/methanol) and alkaline (0.1% triethylamine water/methanol) conditions were 0.2 mg and 5 mg, respectively, based on injection mass-dependent HETP, retention factor and symmetry factor. Finally, the prepared column was applied to the purification of a building block for nucleic acid drugs. This study demonstrated that reversed-phase columns, which can be fabricated from eggshells and an amphiphilic copolymer in an inexpensive and eco-friendly way, have the ability to purify basic compounds and acid-sensitive compounds.

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Papers, etc., Registered in KOARA 【 Display / hide

Presentations 【 Display / hide

  • Eggshell-based Packing Materials for HPLC

    Yuki Hiruta

    HPLC 2022, 

    2022.06

    Poster presentation

  • Development of Furimazine Derivatives for Long-Term Bioluminescence Imaging

    蛭田勇樹

    日本顕微鏡学会 第78回学術講演会, 

    2022.05

    Oral presentation (invited, special)

  • 炭酸カルシウムを母体とするHPLC用充填剤の開発

    蛭田勇樹

    日本化学会第101春季年会, 

    2022.03

    Poster presentation

  • Development of caged luciferin enabling spatiotemporal trans-scale imaging

    Yuki Hiruta

    Pacifichem 2021, 

    2021.12
    -
    2121.12

    Oral presentation (general)

  • Development of stimuli-responsive polymers for diagnostic and therapeutic applications

    Yuki Hiruta

    2021 MRS-T International Conference, 

    2021.11

    Oral presentation (invited, special)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • がんバイオマーカー応答性高分子設計を戦略とした光セラノスティクス薬剤の開発

    2021.04
    -
    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

  • 時空間トランススケールイメージングを可能にする超分子ケージドルシフェリンの開発

    2021.04
    -
    2023.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas, Principal investigator

  • Development of polymer caged luciferin enabling spatiotemporal trans-scale imaging

    2019.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research on Innovative Areas, Principal investigator

  • Development of tumor self-accumulating functionalized theranostics nanoplatform

    2019.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists , Principal investigator

  • 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

    2018.04
    -
    2021.03

    Coinvestigator(s)

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Awards 【 Display / hide

  • Best Poster Award HPLC 2022, San Diego

    2022.06

    Type of Award: Award from international society, conference, symposium, etc.

  • Outstanding Reviewer for journal of Materials Chemistry B

    2021.03

    Type of Award: Award from publisher, newspaper, foundation, etc.

  • Award for Encouragement of Research in Analytical Chemistry

    2020.08, The Japan Society of Analytical Chemistry, Development and application of bioimaging probes based on precision molecular design

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • Award for Encouragement of Research in Polymer Science

    Development of stimuli-responsive polymer materials for application to medical analysis, 2020.05, 公益社団法人高分子学会, 医療分析への応用を志向した刺激応答性高分子材料の開発

    Type of Award: Award from Japanese society, conference, symposium, etc.

  • 日本化学会第99春季年会 優秀講演賞(学術)

    2019.04, 公益財団法人 日本化学会

    Type of Award: Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide

  • SEMINAR IN APPLIED CHEMISTRY

    2023

  • PRACTICAL INSTRUMENTAL ANALYSIS

    2023

  • NANO SCALE SCIENCE JOINT SEMINAR

    2023

  • MATERIAL DESIGN SCIENCE JOINT SEMINAR

    2023

  • LABORATORY IN SCIENCE

    2023

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Memberships in Academic Societies 【 Display / hide

  • クロマトグラフィー科学会会員, 

    2016.01
    -
    Present
  • 高分子学会会員, 

    2016.01
    -
    Present
  • 日本DDS学会会員, 

    2014.04
    -
    Present
  • 日本薬学会会員, 

    2014.01
    -
    Present
  • 日本化学会会員, 

    2010.01
    -
    Present

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Committee Experiences 【 Display / hide

  • 2021.01
    -
    Present

    関東支部常任幹事 , 日本分析化学会

  • 2019.12
    -
    Present

    化学グランプリ・オリンピック委員会 グランプリ小委員会 委員, 公益社団法人日本化学会

  • 2018.04
    -
    Present

    代議員, 日本分析化学会

  • 2017.07
    -
    Present

    幹事, 日本分析化学会関東支部若手の会

  • 2017.01
    -
    2018.12

    関東支部幹事, 日本分析化学会