チッテリオ, ダニエル (チッテリオ ダニエル)

Citterio, Daniel

写真a

所属(所属キャンパス)

理工学部 応用化学科 (矢上)

職名

教授

HP

外部リンク

経歴 【 表示 / 非表示

  • 2002年11月
    -
    2003年09月

    スイス連邦工科大学, 化学センサーセンター, 上級研究員

  • 2005年02月
    -
    2006年01月

    チバ・スペシャルティ・ケミカルズ株式会社(スイス), 特許情報管理部, 特許弁理士

  • 2006年03月
    -
    2007年03月

    慶應義塾大学理工学部, 大学院理工学研究科, 特別研究助教授

  • 2007年04月
    -
    2009年03月

    慶應義塾大学理工学部, 応用化学科, 准教授(有期)

  • 2009年04月
    -
    2014年03月

    慶應義塾大学理工学部, 応用化学科, 准教授

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研究分野 【 表示 / 非表示

  • ナノテク・材料 / 分析化学

研究キーワード 【 表示 / 非表示

  • バイオセンサ

  • 化学センサ

  • 機能性色素

  • 機能材料

 

著書 【 表示 / 非表示

  • Applications of Microfluidic Systems in Biology and Medicine

    山田 健太郎、チッテリオ ダニエル, Springer, Singapore, 2019年04月

    担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    チッテリオ  ダニエル, Springer, 2017年

    担当範囲: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    チッテリオ  ダニエル, Springer, 2015年

    担当範囲: Inkjet Printing of Biomolecules for Biorecognition / 197-235

論文 【 表示 / 非表示

  • Peptide nucleic acid probe-assisted paper-based electrochemical biosensor for multiplexed detection of respiratory viruses

    Lomae A., Teekayupak K., Preechakasedkit P., Pasomsub E., Ozer T., Henry C.S., Citterio D., Vilaivan T., Chailapakul O., Ruecha N.

    Talanta 279 2024年11月

    ISSN  00399140

     概要を見る

    The similar transmission patterns and early symptoms of respiratory viral infections, particularly severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza (H1N1), and respiratory syncytial virus (RSV), pose substantial challenges in the diagnosis, therapeutic management, and handling of these infectious diseases. Multiplexed point-of-care testing for detection is urgently needed for prompt and efficient disease management. Here, we introduce an electrochemical paper-based analytical device (ePAD) platform for multiplexed and label-free detection of SARS-CoV-2, H1N1, and RSV infection using immobilized pyrrolidinyl peptide nucleic acid probes. Hybridization between the probes and viral nucleic acid targets causes changes in the electrochemical response. The resulting sensor offers high sensitivity and low detection limits of 0.12, 0.35, and 0.36 pM for SARS-CoV-2 (N gene), H1N1, and RSV, respectively, without showing any cross-reactivities. The amplification-free detection of extracted RNA from 42 nasopharyngeal swab samples was successfully demonstrated and validated against reverse-transcription polymerase chain reaction (range of cycle threshold values: 17.43–25.89). The proposed platform showed excellent clinical sensitivity (100 %) and specificity (≥97 %) to achieve excellent agreement (κ ≥ 0.914) with the standard assay, thereby demonstrating its applicability for the screening and diagnosis of these respiratory diseases.

  • Basic evaluation of the CRISPR/Cas system stability for application to paper-based analytical devices

    Tanifuji Y., Suzuki H., Tong G., Hiruta Y., Citterio D.

    Analytical Methods 16 ( 25 ) 4143 - 4149 2024年05月

    ISSN  17599660

     概要を見る

    Despite the promising features of the CRISPR/Cas system for application to point-of-care nucleic acid tests, there are only a few reports on its integration into paper-based analytical devices (PADs) for the purpose of assay simplification. In most cases, paper platforms have only been used for the final signal readout in an assay otherwise performed in a test tube. Therefore, there is very limited information on the suitability of the CRISPR/Cas system for on-device reagent storage. To fill this gap, the current work primarily investigated the influence of various factors, including the type of paper, reagent drying method, effect of stabilizers, and storage condition on the storage stability of reagents necessary for CRISPR-based assays on paper substrates, by comparing the fluorescence signal emitted by the trans-cleavage of the dsDNA-activated Cas12a complex. The results obtained in the form of fluorescence signals emitted after trans-cleavage of a ssDNA probe through a dsDNA-activated Cas12a complex on paper substrates showed that CRISPR-related reagents spontaneously dried at room temperature on BSA blocked paper retained over 70% of their initial activity when stored at −20 °C for 28 days, independent of the type of paper substrates, which was improved by the addition of sucrose as a stabilizer. In addition, reagents dried on paper substrates under the optimized conditions exhibited stronger heat tolerance at temperatures above 65 °C compared to their corresponding solutions. This work is expected to contribute to the future development of fully integrated PADs relying on CRISPR/Cas systems for point-of-care applications requiring no additional reagent handling.

  • Rational design of pH-responsive near-infrared spirocyclic cyanines: the effects of substituents and the external environment

    Sakama A., Seo H., Hara J., Shindo Y., Ikeda Y., Oka K., Citterio D., Hiruta Y.

    Chemical Communications 60 ( 46 ) 5984 - 5987 2024年05月

    ISSN  13597345

     概要を見る

    pH-responsive spirocyclic cyanine dyes were designed and synthesized. The equilibrium constant for cyclization (pKcycl) could be rationally controlled by changing the nucleophilic moiety and the side chains. Encapsulation in polymeric micelles inhibited the H-aggregation of the dye, and the pKcycl could be shifted according to the amphiphilic polymer employed.

  • Simplified capture, extraction, and amplification of cellular DNA from water samples

    Kawaguchi M., Aoki H., Kamo H., Miura K., Hiruta Y., Simizu S., Citterio D.

    Analytical Sciences (Analytical Sciences)  40 ( 3 ) 501 - 510 2024年03月

    ISSN  09106340

     概要を見る

    DNA analysis in water samples is attracting attention in various fields. However, conventional methods for DNA analysis require a work-intensive and time-consuming sample pre-treatment. In this study, a simplified pre-treatment method for analyzing DNA in water samples was evaluated. The process consists of filtration, DNA extraction, and amplification, which can be achieved within a short time. In the filtration process, two types of filters, firstly a tissue paper (Kimwipe) and then a glass filter (GF/F), were used in sequence. The first large pore size filter enabled a reduction in filtration time by removing large particulate matter impurities present in river water matrix. Cells spiked into 1 L of river water were recovered at more than 90% within approximately 5 min filtration time. Also, DNA was extracted from the captured cells directly on the surface of the filter in only 5 min. Thus, DNA collection and extraction from a water sample can be completed within about 10 min. Furthermore, PCR amplification was performed directly from DNA-attached filter sections, which greatly reduced the number of required pre-treatment steps. Finally, we succeeded in establishing a simple and fast on-site pre-treatment system by using a hand-driven syringe filtration method. This pre-treatment system is expected to offer the possibility for the future establishment of a rapid and easy DNA analysis method applicable to various types of water samples. Graphical abstract: (Figure presented.)

  • Peroxidase-like Activity of Aptamer-Gold Nanoparticles for Selective and Sensitive Fluorescence Detection of Low-Density Lipoproteins

    Prakobkij A., Saenmuangchin R., Chunta S., Amatatongchai M., Citterio D., Jarujamrus P.

    ACS Applied Nano Materials (ACS Applied Nano Materials)  7 ( 11 ) 12356 - 12365 2024年

     概要を見る

    Low-density lipoprotein cholesterol (LDL-C), commonly called “bad cholesterol”, is crucial to cardiovascular health. Increased LDL-C levels pose a substantial risk to human health. As a result, there is a demand for reliable, affordable, and highly sensitive analytical methods for LDL-C detection. Herein, a facile fluorometric aptasensor for LDL-C detection based on the aptamer-enhanced peroxidase-mimicking activity of gold nanoparticles (AuNPs) has been developed. AuNPs were functionalized with LDL-C-specific thiolated aptamer to enhance their intrinsic peroxidase-like activity, which could effectively catalyze the oxidation of o-phenylenediamine dihydrochloride (OPD) by H2O2 into the yellow-fluorescent product 2,3-diamino phenazine (DAP). The characterization studies confirmed that the presence of the aptamer enhances the affinity of AuNPs toward the OPD substrate, thereby leading to a marked increase in the peroxidase-mimicking activity. Moreover, after being functionalized with an aptamer, increased dispersibility and substrate affinity of AuNPs were achieved. Using LDL-C as a target analyte, under optimum conditions, a linear relationship between smartphone-recorded signal intensity and the logarithm of the analyte concentration was observed in the range of 0.05-1 mg dL-1 and the limit of detection was 0.0230 mg dL-1. The results agree with those obtained by a clinical laboratory method. This proposed method is readily deployable and a promising prototype for diverse diagnostic applications, particularly in biomarker detection within serum or plasma samples. It holds the potential to yield substantial advantages in point-of-care testing.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

総説・解説等 【 表示 / 非表示

  • Editorial: Chemical Sensors for Biomedical Use

    Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

    Frontiers in Chemistry (Frontiers in Chemistry)  9 2021年05月

  • Equipment-free Detection of K<sup>+</sup> on Paper

    Soda Y., Citterio D., Bakker E.

    Chimia (Chimia)  73 ( 11 )  2019年

    ISSN  00094293

  • Paper-based analytical devices

    Citterio D., Kaneta T.

    Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018年

    ISSN  09106340

  • Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

    チッテリオ  ダニエル

    Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017年02月

    記事・総説・解説・論説等(学術雑誌), 共著,  ISSN  1473-0197

  • 紙型マイクロ流体デバイス 検査チップの低コスト化と利便化を目指して

    山田 健太郎、チッテリオ ダニエル

    化学と工業 (日本化学会)  69   120 - 122 2016年

    記事・総説・解説・論説等(その他), 共著

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研究発表 【 表示 / 非表示

  • CRISPR/Cas Assays Integrated into Paper-Based Analytical Devices

    チッテリオ ダニエル

    Matrafured International Conference on Chemical Sensors 2024 (Visegrad) , 

    2024年06月

    口頭発表(招待・特別)

  • Paper-Based Analytical Devices with CRISPR/Cas Signaling

    チッテリオ ダニエル

    International Research Network on Microfluidic Analytical Technology (Bangkok) , 

    2024年04月

    口頭発表(招待・特別), National Research Council of Thailand (NRCT)

  • Paper-Based Analytical Devices with CRISPR/Cas Signal Amplification

    チッテリオ ダニエル

    Pittcon 2024 (San Diego) , 

    2024年02月

    口頭発表(招待・特別), The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy

  • Low-cost and simple analytical devices suitable for point-of-care testing (POCT)

    チッテリオ ダニエル

    The 16th Eurasia Conference on Chemical Sciences (Bangkok) , 

    2023年12月

    口頭発表(招待・特別), The Chemical Society of Thailand

  • Analytical Assays on Paper Platforms: As Simple as Possible

    チッテリオ ダニエル

    Lab-on-a-Chip and Microfluidics Asia 2023 (Narita) , 

    2023年10月

    口頭発表(基調), SelectBIO

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競争的研究費の研究課題 【 表示 / 非表示

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022年04月
    -
    2025年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ ダニエル, 基盤研究(B), 補助金,  研究代表者

  • 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

    2018年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ, ダニエル, 基盤研究(B), 補助金,  研究代表者

受賞 【 表示 / 非表示

  • 学術賞

    チッテリオ ダニエル, 2022年03月, 日本化学会, 化学・生化学センシングのため機能性色素および紙基板分析デバイスの開発

    受賞区分: 国内学会・会議・シンポジウム等の賞

 

担当授業科目 【 表示 / 非表示

  • 応用化学輪講

    2024年度

  • 機器分析総論

    2024年度

  • 応用化学系英語

    2024年度

  • ナノスケール科学ジョイントセミナー

    2024年度

  • マテリアルデザイン科学ジョイントセミナー

    2024年度

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担当経験のある授業科目 【 表示 / 非表示

  • 応用化学系英語

    慶應義塾

    2016年04月
    -
    2017年03月

    通年, 講義, 専任

  • 基礎化学実験

    慶應義塾

    2016年04月
    -
    2017年03月

    秋学期, 実習・実験, 専任

  • 応用化学実験

    慶應義塾

    2016年04月
    -
    2017年03月

    春学期, 実習・実験, 専任

  • 機能物質概論

    慶應義塾

    2016年04月
    -
    2017年03月

    春学期, 講義, 専任

  • 機器分析総論

    慶應義塾

    2016年04月
    -
    2017年03月

    春学期, 講義, 専任

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所属学協会 【 表示 / 非表示

  • 日本化学会, 

    2006年04月
    -
    継続中
  • 日本分析化学会, 

    2006年04月
    -
    継続中
  • 米国化学会, 

    2007年03月
    -
    継続中
  • 英国王立化学協会 (RSC) (フェロー), 

    2016年02月
    -
    継続中

委員歴 【 表示 / 非表示

  • 2017年04月
    -
    継続中

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode