チッテリオ, ダニエル (チッテリオ, ダニエル)

Citterio, Daniel

写真a

所属(所属キャンパス)

理工学部 応用化学科 (矢上)

職名

教授

メールアドレス

メールアドレス

HP

外部リンク

経歴 【 表示 / 非表示

  • 2002年11月
    -
    2003年09月

    スイス連邦工科大学, 化学センサーセンター, 上級研究員

  • 2005年02月
    -
    2006年01月

    チバ・スペシャルティ・ケミカルズ株式会社(スイス), 特許情報管理部, 特許弁理士

  • 2006年03月
    -
    2007年03月

    慶應義塾大学理工学部, 大学院理工学研究科, 特別研究助教授

  • 2007年04月
    -
    2009年03月

    慶應義塾大学理工学部, 応用化学科, 准教授(有期)

  • 2009年04月
    -
    2014年03月

    慶應義塾大学理工学部, 応用化学科, 准教授

全件表示 >>

 

研究分野 【 表示 / 非表示

  • 分析化学

研究キーワード 【 表示 / 非表示

  • バイオセンサ

  • 化学センサ

  • 機能性色素

  • 機能材料

 

著書 【 表示 / 非表示

  • Applications of Microfluidic Systems in Biology and Medicine

    山田 健太郎、チッテリオ ダニエル, Springer, Singapore, 2019年04月

    担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    チッテリオ  ダニエル, Springer, 2017年

    担当範囲: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    チッテリオ  ダニエル, Springer, 2015年

    担当範囲: Inkjet Printing of Biomolecules for Biorecognition / 197-235

論文 【 表示 / 非表示

  • Biothiol-activatable bioluminescent coelenterazine derivative for molecular imaging in vitro and in vivo

    Nomura N., Nishihara R., Nakajima T., Kim S., Iwasawa N., Hiruta Y., Nishiyama S., Sato M., Citterio D., Suzuki K.

    Analytical Chemistry (Analytical Chemistry)  91 ( 15 ) 9546 - 9553 2019年08月

    ISSN  00032700

     概要を見る

    © 2019 American Chemical Society. There is a high demand for sensitive biothiol probes targeting cysteine, glutathione, and homocysteine. These biothiols are known as playing essential roles to maintain homeostasis and work as indicators of many diseases. This work presents a bioluminescent probe (named AMCM) to detect biothiols in live mammalian cells and in vivo with a limit of detection of 0.11 μM for cysteine in solution and high selectivity for biothiols, making it suitable for real-time biothiol detection in biological systems. Upon application to live cells, AMCM showed low cytotoxicity and sensitively reported bioluminescence in response to changes of biothiol levels. Furthermore, a bioluminescence resonance energy transfer system consisting of AMCM combined with the near-infrared fluorescent protein iRFP713 was applied to in vivo imaging, with emitted tissue-permeable luminescence in living mice. In summary, this work demonstrates that AMCM is of high practical value for the detection of biothiols in living cells and for deep tissue imaging in living animals.

  • Disposable electrochemical biosensor based on surface-modified screen-printed electrodes for organophosphorus pesticide analysis

    Hua Q., Ruecha N., Hiruta Y., Citterio D.

    Analytical Methods (Analytical Methods)  11 ( 27 ) 3439 - 3445 2019年07月

    ISSN  17599660

     概要を見る

    © 2019 The Royal Society of Chemistry. A disposable enzymatic electrochemical biosensor for amperometric organophosphorus pesticide determination based on a screen-printed carbon electrode (SPCE) platform was successfully developed. The working electrode consists of acetylcholine esterase (AChE) immobilized onto the surface of multi-walled carbon nanotube (MWCNT), chitosan (CS) and gold nanoparticle (AuNP)-modified SPCE. Ferricyanide was used as a redox mediator in solution. Two linear dynamic response ranges from 0.01 to 10 μg L-1 and from 10 to 100 μg L-1 were found for paraoxon-ethyl, with a detection limit of 0.03 μg L-1 (calculated as the amount of pesticide resulting in 10% of enzyme inhibition). High sensitivity was achieved due to a synergistic effect of AuNPs and MWCNTs deposited on the SPCE surface. Moreover, 83% of enzyme activity was retained after a dry storage period of 49 days (4 °C). Finally, the results of the electrochemical biosensor for paraoxon-ethyl-spiked spinach sample analysis showed a good agreement with those obtained from conventional HPLC.

  • Molecular imaging of retinoic acids in live cells using single-chain bioluminescence probes

    Kim S., Fujii R., Nishihara R., Bose R., Citterio D., Suzuki K., Massoud T., Paulmurugan R.

    ACS Combinatorial Science (ACS Combinatorial Science)  21 ( 6 ) 473 - 481 2019年06月

    ISSN  21568952

     概要を見る

    © 2019 American Chemical Society. Retinoic acid (RA) is a key metabolite necessary for embryonic development and differentiation in vertebrates. We demonstrate the utility of genetically encoded, ligand-activatable single-chain bioluminescence probes for detecting RAs from different biological sources. We examined 13 different molecular designs to identify an efficient single-chain probe that can quantify RA with significant sensitivity. The optimal probe consisted of four components: the N- and C-terminal fragments of artificial luciferase variant-16 (ALuc16), the ligand binding domain of retinoic acid receptor α (RARα LBD), and an LXXLL interaction motif. This probe showed a 5.2-fold greater bioluminescence intensity in response to RA when compared to the vehicle control in live mammalian cells. The probe was highly selective to all-trans-RA (at-RA), and highly sensitive in determining at-RA levels in cells derived from tumor xenografts created using MDA-MB-231 cells engineered to stably express the probe. We also detected RA levels in serum and cerebrospinal fluid. Using this probe, the detection limit for at-RA was 10-9.5 M, with a linear range of two orders. We present a highly useful technique to quantitatively image endogenous at-RA levels in live mammalian cells expressing novel single-chain bioluminescence probes.

  • Printed low-cost microfluidic analytical devices based on a transparent substrate

    Fujisaki S., Shibata H., Yamada K., Suzuki K., Citterio D.

    Analyst (Analyst)  144 ( 8 ) 2746 - 2754 2019年04月

    ISSN  00032654

     概要を見る

    © 2019 The Royal Society of Chemistry. This work describes the development of a microfluidic analytical device prepared on a transparent OHP film substrate, named the microfluidic transparent film-based analytical device (μTFAD). Printing technologies including wax printing for microchannel patterning and inkjet printing for chemical assay component deposition have been employed for the μTFAD fabrication. The fully printed μTFAD allowed gravity-assisted pump-free transportation of the sample liquid (50 μL) and an absorbance measurement-based iron ion (Fe 2+ ) assay using nitroso-PSAP as the colorimetric reagent within a wax-patterned microfluidic structure. By measuring absorbance values at the Fe 2+ -nitroso-PSAP complex-specific wavelength (756 nm), a response curve with a linear range of 0-200 μM was obtained. The limit of detection (1.18 μM) obtained with the proposed μTFADs was comparable to the results achieved with a conventional 96-well microplate assay (0.92 μM) and lower than that in the case of digital colour analysis-assisted filter paper spot tests (7.71 μM) or the absorbance analysis of refractive index-matched translucent filter paper spots (37.2 μM). In addition, highly selective Fe 2+ detection has been achieved in the presence of potentially interfering metal ions (Cu 2+ , Co 2+ , Ni 2+ ) without the use of any masking reagents, owing to the selection of the target complex-specific wavelength in the absorbance measurement on μTFADs.

  • Equipment-Free Detection of K <sup>+</sup> on Microfluidic Paper-Based Analytical Devices Based on Exhaustive Replacement with Ionic Dye in Ion-selective Capillary Sensors

    Soda Y., Citterio D., Bakker E.

    ACS Sensors (ACS Sensors)  4 ( 3 ) 670 - 677 2019年03月

     概要を見る

    © 2019 American Chemical Society. A distance-based analysis of potassium ion (K + ) is introduced that is performed on a microfluidic paper-based analytical device (μPAD) coupled to an ion-selective capillary sensor. The concept is based on two sequential steps, the selective replacement of analyte ion with an ionic dye, and the detection of this dye in a distance-based readout on paper. To achieve the first step, the capillary sensor holds a poly(vinyl chloride) (PVC) membrane film layer plasticized by dioctyl sebacate (DOS) that contains the potassium ionophore valinomycin, a lipophilic cation-exchanger and the ionic indicator Thioflavin T (ThT) on its inner wall. Upon introduction of the sample, K + in the aqueous sample solution is quantitatively extracted into the film membrane and replaced with ThT. To convert the ion exchange signal into a distance-based analysis, this solution was dropped onto the inlet area of a μPAD to flow the ThT along a channel defined by wax printing, resulting in the electrostatic binding of ThT to the cellulose carboxylic groups. The initial amount of K + determines the amount of ThT in the aqueous solution after ion-exchange, and consequently the distance of ThT-colored area reflects the sample K + concentration. The ion exchange reaction was operated in a so-called "exhaustive sensing mode" and gave a distinct response in a narrow range of K + concentration (1-6 mM) that cannot be achieved by the classical optode sensing mode. The absence of hydrogen ions from the equilibrium competition of the capillary sensor contributed to a complete pH-independence, unlike conventional optodes that contain a pH sensitive indicator. A very high selectivity for K + over Na + and Ca 2+ has been confirmed in separate solutions and mixed solutions tests. K + measurements in pooled serum samples at concentrations between 2 and 6 mM are successfully demonstrated on a temperature controlled support.

全件表示 >>

KOARA(リポジトリ)収録論文等 【 表示 / 非表示

総説・解説等 【 表示 / 非表示

  • Paper-based analytical devices

    Citterio D., Kaneta T.

    Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018年

    ISSN  09106340

  • Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

    チッテリオ  ダニエル

    Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017年02月

    総説・解説(学術雑誌), 共著,  ISSN  1473-0197

  • 紙型マイクロ流体デバイス 検査チップの低コスト化と利便化を目指して

    山田 健太郎、チッテリオ ダニエル

    化学と工業 (日本化学会)  69   120 - 122 2016年

    総説・解説(その他), 共著

  • インクジェット技術によるペーパーマイクロ流体デバイスの開発

    石井 政憲、城戸 滉太、太田 力、柴田 寛之、 山田 健太郎、鈴木 孝治、チッテリオ ダニエル

    日本画像学会誌 (日本画像学会)  55   94 - 105 2016年

    総説・解説(その他), 共著

  • Inkjet Printed Microfluidic Paper-Based Analytical Devices (µPADs)

    チッテリオ ダニエル

    Angewandte Chemie International Edition (Wiley)  54 ( 18 ) 5294 - 5310 2015年04月

    総説・解説(学術雑誌), 共著,  ISSN  1521-3773

研究発表 【 表示 / 非表示

  • Paper- and Thread-Based Immunoassay Devices with Colorimetric Bioluminescence Signaling

    チッテリオ ダニエル

    Pittcon 2019 (フィラデルフィア(アメリカ)) , 2019年03月, 口頭(招待・特別), The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy

  • Capillary Flow-Driven Analytical Devices for Sample-in-Signal-Out Assaying of Clinically Relevant Analytes

    チッテリオ ダニエル

    Flow Analysis XIV (バンコク(タイ)) , 2018年12月, 口頭(基調), The Chemical Society of Thailand

  • Paper-Based Analytical Device for the Detection of Antibodies in Biological Samples

    チッテリオ ダニエル

    PERCH - CIC Congress X (パッタヤー(タイ)) , 2018年07月, 口頭(招待・特別), Department of Chemistry, Faculty of Science, Mahidol University

  • Paper-Based Devices for Antibody Detection in Whole Blood with Bioluminescent Sensing Proteins

    チッテリオ ダニエル

    第78回分析化学討論会 (山口大学) , 2018年05月, 口頭(招待・特別), 日本分析化学会

  • Paper-Based Devices for Antibody Detection in Whole Blood with Bioluminescent Sensing Proteins

    チッテリオ  ダニエル

    Europt(r)ode 2018 (Naples, Italy) , 2018年03月, 口頭(一般)

全件表示 >>

競争的資金等の研究課題 【 表示 / 非表示

  • 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

    2018年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ, ダニエル, 基盤研究(B), 補助金,  代表

 

担当授業科目 【 表示 / 非表示

  • 応用化学輪講

    2021年度

  • 機器分析総論

    2021年度

  • 応用化学系英語

    2021年度

  • ナノスケール科学ジョイントセミナー

    2021年度

  • マテリアルデザイン科学ジョイントセミナー

    2021年度

全件表示 >>

担当経験のある授業科目 【 表示 / 非表示

  • 応用化学系英語

    慶應義塾, 2016年度, 通年, 専門科目, 講義, 専任

  • 基礎化学実験

    慶應義塾, 2016年度, 秋学期, 専門科目, 実習・実験, 専任

  • 応用化学実験

    慶應義塾, 2016年度, 春学期, 専門科目, 実習・実験, 専任

  • 機能物質概論

    慶應義塾, 2016年度, 春学期, 専門科目, 講義, 専任

  • 機器分析総論

    慶應義塾, 2016年度, 春学期, 専門科目, 講義, 専任

全件表示 >>

 

所属学協会 【 表示 / 非表示

  • 日本化学会, 

    2006年04月
    -
    継続中
  • 日本分析化学会, 

    2006年04月
    -
    継続中
  • 米国化学会, 

    2007年03月
    -
    継続中
  • 英国王立化学協会 (RSC) (フェロー), 

    2016年02月
    -
    継続中

委員歴 【 表示 / 非表示

  • 2017年04月
    -
    継続中

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode