チッテリオ，　ダニエル （チッテリオ　ダニエル）

Citterio, Daniel

写真a

所属（所属キャンパス）

理工学部 応用化学科 （矢上）

職名

教授

HP

外部リンク
このページの先頭へ▲

経歴 【 表示 ／ 非表示 】

  • 2002年11月
    -
    2003年09月

    スイス連邦工科大学, 化学センサーセンター, 上級研究員

  • 2005年02月
    -
    2006年01月

    チバ・スペシャルティ・ケミカルズ株式会社（スイス）, 特許情報管理部, 特許弁理士

  • 2006年03月
    -
    2007年03月

    慶應義塾大学理工学部, 大学院理工学研究科, 特別研究助教授

  • 2007年04月
    -
    2009年03月

    慶應義塾大学理工学部, 応用化学科, 准教授（有期）

  • 2009年04月
    -
    2014年03月

    慶應義塾大学理工学部, 応用化学科, 准教授

全件表示 >>

このページの先頭へ▲
 

研究分野 【 表示 ／ 非表示 】

  • ナノテク・材料 / 分析化学

このページの先頭へ▲

研究キーワード 【 表示 ／ 非表示 】

  • バイオセンサ

  • 化学センサ

  • 機能性色素

  • 機能材料

このページの先頭へ▲
 

著書 【 表示 ／ 非表示 】

  • Applications of Microfluidic Systems in Biology and Medicine

    Yanawut Manmana, Kentaro Yamada, Daniel Citterio, Springer Nature Singapore Pte Ltd., 2024年

    担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 443-493

  • Applications of Microfluidic Systems in Biology and Medicine

    山田　健太郎、チッテリオ　ダニエル, Springer, Singapore, 2019年04月

    担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    チッテリオ　 ダニエル, Springer, 2017年

    担当範囲: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    チッテリオ　 ダニエル, Springer, 2015年

    担当範囲: Inkjet Printing of Biomolecules for Biorecognition / 197-235

このページの先頭へ▲

論文 【 表示 ／ 非表示 】

  • Ratiometric fluorometry on microfluidic paper-based analytical device for simultaneous glucose and cholesterol detection using MnFe-layered double hydroxides as peroxidase mimic

    Kitchawengkul N., Prakobkij A., Saenmuangchin R., Citterio D., Nacapricha D., Jarujamrus P.

    Sensors and Actuators B: Chemical 435 2025年07月

    ISSN  09254005

    　概要を見る

    A highly sensitive ratiometric fluorescence sensing system was developed for simultaneous glucose and total cholesterol (TC) detection in whole blood using MnFe-layered double hydroxides (MnFe-LDHs) as a peroxidase mimic, combined with an o-phenylenediamine (OPD) substrate and nitrogen-doped graphene quantum dots (N-GQDs). The detection platform, an X-shaped laminated microfluidic paper-based analytical device (XL-μPAD), was fabricated via laser printing and cutting. The MnFe-LDHs' large surface area and layered structure provide a high affinity for OPD, with a Michaelis–Menten constant (KM) of 0.0127 mmol L−1. Upon placing a drop of blood on the XL-μPAD sample pad, the enzymatic reactions of glucose and TC produce H2O2, which MnFe-LDHs convert to hydroxyl radicals (•OH). These radicals oxidize OPD into fluorescent 2,3-diamino phenazine (DAP) with emission at 560 nm. Meanwhile, the N-GQDs emit fluorescence at 415 nm, which is quenched by DAP through the inner filter effect (IFE) and dynamic quenching, enabling ratiometric sensing via the intensity ratio (I560/I415). As H2O2 levels increase, a visible green emission appears, correlating with glucose and TC levels. This XL-μPAD system demonstrates promising potential as a portable device for multiplex biomarker detection and diagnostic applications.

  • Origami Paper-Based Immunoassay Device with CRISPR/Cas12a Signal Amplification

    Suzuki H., Tong G., Nath P., Hiruta Y., Citterio D.

    ACS Sensors 10 （ 3 ） 1811 - 1821 2025年03月

    　概要を見る

    In clinical diagnosis, the determination of target proteins at low concentration levels is generally performed by immunoassays, such as the enzyme-linked immunosorbent assay (ELISA), which is a time-consuming process. To date, paper-based ELISA platforms enabling faster and less expensive analysis have been developed, but their important issue for clinical applications is the limited sensitivity compared to conventional ELISA. To address this challenge, this paper introduces a simple, rapid, and highly sensitive detection method for non-nucleic acid targets achieved by integrating the CRISPR/Cas12a system into paper-based ELISA. An origami-type paper-based device enabling simple assay operation has been designed, and the detection of targets on the paper substrates is based on observing the fluorescence signal induced by the CRISPR/Cas12a enzyme cleaving a probe single-stranded DNA (ssDNA) labeled with fluorophore and quencher (FQ reporter). To enhance sensitivity, antibodies labeled with a network of multiple DNA activating the CRISPR/Cas12a enzyme have been utilized as detection antibodies. As a result, the developed device successfully boosted the detection sensitivity for both human IgG and the hepatitis B virus surface antigen (HBsAg). In particular, the limit of detection (LOD) for HBsAg was estimated to be 12 pg/mL, representing over 10-fold higher sensitivity compared with commercially available HBsAg ELISA kits (LOD: 200 pg/mL). In addition, the fluorescence response toward porcine whole blood samples containing different HBsAg concentrations was also confirmed by capturing images with a smartphone, followed by quantitative data analysis. These results demonstrate the potential applicability of the proposed platform for clinical tests at the point of care.

  • Bioluminescence readout lateral flow immunoassay using nanobody targeting aflatoxin B1

    Takahashi S., Hiruta Y., Citterio D.

    Analyst 150 （ 8 ） 1563 - 1570 2025年03月

    ISSN  00032654

    　概要を見る

    Multiple signal detection methods are known for lateral flow immunoassays (LFIAs), with colorimetric approaches dominating the field. However, their limited sensitivity is a remaining challenge. Fluorescence-based signaling is regarded as a more sensitive method, but it comes at the cost of partial sacrifice of the user-friendliness of LFIAs due to the requirement of an excitation light source. In this context, bioluminescence providing an inherently high signal to noise ratio without the need of excitation light could be an attractive alternative. But only a few studies have demonstrated the application of bioluminescence signaling in LFIAs. This work aimed at the development of a simple bioluminescence-based LFIA for the detection of aflatoxin B1 (AFB1), used as a model target in a competitive LFIA format. Signal transduction was achieved by nanobody-nanoluciferase (Nluc) fusion proteins. These small-sized recombinant heavy-chain-only antibodies derived from the camelidae family directly linked with the Nluc enzyme produce high intensity glow-type bioluminescence in combination with the furimazine substrate. LFIA devices consisting of a sample pad, nitrocellulose membrane and absorbent pad with AFB1-BSA conjugate deposited at the test line on the nitrocellulose membrane, achieved an LOD of 0.26 ng mL−1 for aqueous AFB1 solutions pre-mixed with Nanobody-Nluc and bioluminescence emission observed on an imaging system. More user-friendly LFIA devices with integrated conjugate pad and pre-deposited Nanobody-Nluc provided clear AFB1 concentration-dependent bioluminescence signals with low background and enabled readout with a standard digital camera, resulting in an LOD of 1.12 ng mL−1. Finally, the LFIA strips have been applied in AFB1-spiked oat milk samples. The LOD of 4.09 ng mL−1 achieved in the real sample matrix is well below the maximum allowable residual concentration of AFB1 in the U.S. (20 ng mL−1).

  • Ratiometric Imaging for Quantification of Elevated Ca<sup>2+</sup> in Neurons Using Synthetic Low-Affinity Fluorescent Probe

    Kuronuma Y., Shindo Y., Kumada R., Sakama A., Citterio D., Oka K., Hiruta Y.

    ACS Chemical Neuroscience 16 （ 4 ） 649 - 658 2025年02月

    　概要を見る

    The availability of various calcium ion (Ca2+) fluorescent probes has contributed to revealing physiological events related to intracellular Ca2+. However, conventional probes face challenges for quantitatively and selectively visualizing high Ca2+ concentrations in cells induced by any stimuli, including biomolecules or electrical signal that disrupt Ca2+ homeostasis. In this report, we designed and synthesized a low-affinity ratiometric Ca2+ probe, KLCA-Fura, utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a ligand, for which we recently demonstrated the suitability as a new low-affinity ligand for Ca2+. KLCA-Fura showed a blue shift in excitation wavelength with increasing Ca2+ concentration based on the intramolecular charge transfer (ICT). Its affinity for Ca2+ is lower than commercially available conventional Ca2+ probes. Furthermore, the selectivity for Ca2+ and the fluorescence intensity were considered sufficient to accurately detect Ca2+. The corresponding acetoxymethyl ester, KLCA-FuraAM, was synthesized for intracellular imaging and applied to Ca2+ quantification in neurons. KLCA-FuraAM enabled quantitative ratiometric monitoring of the two-step Ca2+ concentration increase induced by glutamate stimulation. While this two-step response was not clearly observed with a commercially available low-affinity ratiometric Ca2+ probe, Fura-FF, KLCA-FuraAM has demonstrated the potential to quantitatively visualize the behavior of high Ca2+ concentrations. The ratiometric low-affinity Ca2+ probe, KLCA-Fura, is expected to be a powerful tool for discovering new functions of Ca2+ in neurons.

  • Present and future of smartphone-coupled chemiluminescence and electrochemiluminescence assays: a mini-review

    Dutta C., Citterio D., Nath P.

    Analyst 150 （ 6 ） 1033 - 1047 2025年02月

    ISSN  00032654

    　概要を見る

    The convergence of smartphones with chemiluminescence and electrochemiluminescence (CL/ECL) assays marks a transformative leap in the realm of sensing technologies. The traditional CL/ECL assays, known for their high sensitivity and versatility, find extensive applications in medical diagnostics, environmental monitoring, food safety, and forensic sciences. However, these techniques have long been constrained due to the requirement of expensive instrumentation and complex reagent handling and hence their accessibility within certain environments is limited. In an era where rapid, accurate, and routine analysis is critical, smartphone-enabled CL/ECL systems offer substantial advantages over conventional analytical methods. By leveraging the universal accessibility and technological sophistication of smartphones and combining them with CL/ECL-based sensing, the smartphone has evolved into a cost-efficient and accessible analytical platform. The ability of the combined platform to conduct on-site analysis in real-time with minimal effort has emerged as a game-changer, particularly in low-resource settings. This mini-review explores the rapid evolution of smartphone-coupled CL/ECL systems over the last five years. The article covers the areas where the combined platform has been implemented in recent years for various sensing applications. The review further identifies key challenges that are associated with such combined platforms and finally highlights the future perspectives of the present topic.

全件表示 >>

このページの先頭へ▲

KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

このページの先頭へ▲

総説・解説等 【 表示 ／ 非表示 】

全件表示 >>

このページの先頭へ▲

研究発表 【 表示 ／ 非表示 】

  • ポイント・オブ・ケア検査用の紙基板分析デバイス：ユーザーフレンドリーかつ高感度にできるか？

    チッテリオ　ダニエル

    日本化学会 第105春季年会 (2025) （大阪） , 

    2025年03月
    , 

    口頭発表（招待・特別）, 日本化学会

  • CRISPR/Cas for highly sensitive paper-based analytical assays

    チッテリオ　ダニエル

    Pure and Applied Chemistry International Conference PACCON （Khao Yai） , 

    2025年02月
    , 

    口頭発表（基調）, Chemical Society of Thailand

  • CRISPR/Cas assays fully integrated into paper-based platforms

    チッテリオ　ダニエル

    Lab-on-a-Chip & Microfluidics Asia 2024 （Narita） , 

    2024年11月
    , 

    口頭発表（招待・特別）, SelectBIO

  • CRISPR/Cas Assays Integrated into Paper-Based Analytical Devices

    チッテリオ　ダニエル

    Matrafured International Conference on Chemical Sensors 2024 （Visegrad） , 

    2024年06月
    , 

    口頭発表（招待・特別）

  • Paper-Based Analytical Devices Relying on CRISPR/Cas Signaling

    チッテリオ　ダニエル

    International Symposium on Science and Education （Taipei） , 

    2024年06月
    , 

    口頭発表（招待・特別）, National Taiwan Normal University

全件表示 >>

このページの先頭へ▲

競争的研究費の研究課題 【 表示 ／ 非表示 】

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022年04月
    -
    2025年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ ダニエル, 基盤研究(B), 補助金,  研究代表者

  • 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

    2018年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ,　ダニエル, 基盤研究(B), 補助金,  研究代表者

このページの先頭へ▲

受賞 【 表示 ／ 非表示 】

  • 学術賞

    チッテリオ　ダニエル, 2022年03月, 日本化学会, 化学・生化学センシングのため機能性色素および紙基板分析デバイスの開発

    受賞区分： 国内学会・会議・シンポジウム等の賞

このページの先頭へ▲
 

担当授業科目 【 表示 ／ 非表示 】

  • 分析化学

    2025年度

  • 応用化学輪講

    2025年度

  • 機器分析総論

    2025年度

  • 応用化学系英語

    2025年度

  • ナノスケール科学ジョイントセミナー

    2025年度

全件表示 >>

このページの先頭へ▲

担当経験のある授業科目 【 表示 ／ 非表示 】

  • 化学・バイオセンサーとセンシングマテリアル

    慶應義塾

    2016年04月
    -
    2017年03月
    , 

    春学期, 講義, 専任

  • 応用化学系英語

    慶應義塾

    2016年04月
    -
    2017年03月
    , 

    通年, 講義, 専任

  • 基礎化学実験

    慶應義塾

    2016年04月
    -
    2017年03月
    , 

    秋学期, 実習・実験, 専任

  • 応用化学実験

    慶應義塾

    2016年04月
    -
    2017年03月
    , 

    春学期, 実習・実験, 専任

  • 機能物質概論

    慶應義塾

    2016年04月
    -
    2017年03月
    , 

    春学期, 講義, 専任

全件表示 >>

このページの先頭へ▲
 

所属学協会 【 表示 ／ 非表示 】

  • 日本化学会, 

    2006年04月
    -
    継続中

  • 日本分析化学会, 

    2006年04月
    -
    継続中

  • 米国化学会, 

    2007年03月
    -
    継続中

  • 英国王立化学協会 (RSC) (フェロー), 

    2016年02月
    -
    継続中
このページの先頭へ▲

委員歴 【 表示 ／ 非表示 】

  • 2017年04月
    -
    継続中

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode

このページの先頭へ▲