慶應義塾研究者情報データベース

経歴 【 表示 ／ 非表示 】

経歴 【 表示 ／ 非表示 】

• 2002年11月

  -

  2003年09月

  スイス連邦工科大学, 化学センサーセンター, 上級研究員

• 2005年02月

  -

  2006年01月

  チバ・スペシャルティ・ケミカルズ株式会社（スイス）, 特許情報管理部, 特許弁理士

• 2006年03月

  -

  2007年03月

  慶應義塾大学理工学部, 大学院理工学研究科, 特別研究助教授

• 2007年04月

  -

  2009年03月

  慶應義塾大学理工学部, 応用化学科, 准教授（有期）

• 2009年04月

  -

  2014年03月

  慶應義塾大学理工学部, 応用化学科, 准教授

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研究分野 【 表示 ／ 非表示 】

研究分野 【 表示 ／ 非表示 】

• ナノテク・材料 / 分析化学

研究キーワード 【 表示 ／ 非表示 】

研究キーワード 【 表示 ／ 非表示 】

• バイオセンサ

• 化学センサ

• 機能性色素

• 機能材料

著書 【 表示 ／ 非表示 】

著書 【 表示 ／ 非表示 】

• Applications of Microfluidic Systems in Biology and Medicine

  山田　健太郎、チッテリオ　ダニエル, Springer, Singapore, 2019年04月

  担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

• Materials for Chemical Sensing

  チッテリオ　 ダニエル, Springer, 2017年

  担当範囲: (Bio)Chemical Sensors Based on Paper / 29-74

• Design of Polymeric Platforms for Selective Biorecognition

  チッテリオ　 ダニエル, Springer, 2015年

  担当範囲: Inkjet Printing of Biomolecules for Biorecognition / 197-235

論文 【 表示 ／ 非表示 】

論文 【 表示 ／ 非表示 】

• Simplified capture, extraction, and amplification of cellular DNA from water samples

  Kawaguchi M., Aoki H., Kamo H., Miura K., Hiruta Y., Simizu S., Citterio D.

  Analytical Sciences （Analytical Sciences)  40 （ 3 ） 501 - 510 2024年03月

  ISSN  09106340

  　概要を見る

  DNA analysis in water samples is attracting attention in various fields. However, conventional methods for DNA analysis require a work-intensive and time-consuming sample pre-treatment. In this study, a simplified pre-treatment method for analyzing DNA in water samples was evaluated. The process consists of filtration, DNA extraction, and amplification, which can be achieved within a short time. In the filtration process, two types of filters, firstly a tissue paper (Kimwipe) and then a glass filter (GF/F), were used in sequence. The first large pore size filter enabled a reduction in filtration time by removing large particulate matter impurities present in river water matrix. Cells spiked into 1 L of river water were recovered at more than 90% within approximately 5 min filtration time. Also, DNA was extracted from the captured cells directly on the surface of the filter in only 5 min. Thus, DNA collection and extraction from a water sample can be completed within about 10 min. Furthermore, PCR amplification was performed directly from DNA-attached filter sections, which greatly reduced the number of required pre-treatment steps. Finally, we succeeded in establishing a simple and fast on-site pre-treatment system by using a hand-driven syringe filtration method. This pre-treatment system is expected to offer the possibility for the future establishment of a rapid and easy DNA analysis method applicable to various types of water samples. Graphical abstract: (Figure presented.)

  • Access to Document (DOI)

• Peroxidase-like Activity of Aptamer-Gold Nanoparticles for Selective and Sensitive Fluorescence Detection of Low-Density Lipoproteins

  Prakobkij A., Saenmuangchin R., Chunta S., Amatatongchai M., Citterio D., Jarujamrus P.

  ACS Applied Nano Materials （ACS Applied Nano Materials)  2024年

  　概要を見る

  Low-density lipoprotein cholesterol (LDL-C), commonly called “bad cholesterol”, is crucial to cardiovascular health. Increased LDL-C levels pose a substantial risk to human health. As a result, there is a demand for reliable, affordable, and highly sensitive analytical methods for LDL-C detection. Herein, a facile fluorometric aptasensor for LDL-C detection based on the aptamer-enhanced peroxidase-mimicking activity of gold nanoparticles (AuNPs) has been developed. AuNPs were functionalized with LDL-C-specific thiolated aptamer to enhance their intrinsic peroxidase-like activity, which could effectively catalyze the oxidation of o-phenylenediamine dihydrochloride (OPD) by H2O2 into the yellow-fluorescent product 2,3-diamino phenazine (DAP). The characterization studies confirmed that the presence of the aptamer enhances the affinity of AuNPs toward the OPD substrate, thereby leading to a marked increase in the peroxidase-mimicking activity. Moreover, after being functionalized with an aptamer, increased dispersibility and substrate affinity of AuNPs were achieved. Using LDL-C as a target analyte, under optimum conditions, a linear relationship between smartphone-recorded signal intensity and the logarithm of the analyte concentration was observed in the range of 0.05-1 mg dL-1 and the limit of detection was 0.0230 mg dL-1. The results agree with those obtained by a clinical laboratory method. This proposed method is readily deployable and a promising prototype for diverse diagnostic applications, particularly in biomarker detection within serum or plasma samples. It holds the potential to yield substantial advantages in point-of-care testing.

  • Access to Document (DOI)

• Development of Phosphinate Ligand-Based Low-Affinity Ca²⁺ Fluorescent Probes and Application to Intracellular Ca²⁺ Imaging

  Kumada R., Sakama A., Shindo Y., Kuronuma Y., Iwasawa N., Citterio D., Oka K., Hiruta Y.

  Analytical Chemistry （Analytical Chemistry)  95 （ 45 ） 16683 - 16691 2023年11月

  ISSN  00032700

  　概要を見る

  Divalent metal cations such as calcium ion (Ca2+) and magnesium ion (Mg2+) are indispensable to the regulation of various cellular activities. In this research, we developed the KLCA series utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a target binding site, which was reported recently as a highly free Mg2+-selective ligand. KLCA-301 with orange fluorescence based on a rhodamine fluorophore and KLCA-501 with near-infrared (NIR) fluorescence based on a Si-rhodamine fluorophore were synthesized, intended for application to multicolor imaging. The evaluation of the fluorescence response to Ca2+ and Mg2+ of the KLCA series indicated the applicability as low-affinity Ca2+ probes. While KLCA-301 mainly localized in the cytosol in cultured rat hippocampal neurons, KLCA-501 localized to the cytosol and granular organelles in neurons. Comparison of the fluorescence response of KLCA-301 and the high-affinity Ca2+ probe Fluo-4 upon stimulation by glutamate in stained neurons revealed that KLCA-301 could reflect the secondary large rise of intracellular Ca2+, which Fluo-4 could not detect. In addition, KLCA-501 showed a fluorescence response similar to the low-affinity Ca2+ probe Fluo-5N upon stimulation by glutamate in stained neurons, concluding that KLCA-301 and KLCA-501 could be used as low-affinity Ca2+ probes. The KLCA series offers new options for low-affinity Ca2+ probes. Moreover, KLCA-501 achieved simultaneous visualization of the change in Ca2+ and ATP concentrations and also in mitochondrial inner membrane potential in neurons. KLCA-501 is expected to be a strong tool that enables simultaneous multicolor imaging of multiple targets and elucidation of their relationship in cells.

  • Access to Document (DOI)

• Cation/anion-exchange mode switching chromatography utilizing pH-responsive mixed charge polymer-modified silica beads

  Kaku T., Deura K., Yoshii T., Citterio D., Hiruta Y.

  Molecular Systems Design and Engineering （Molecular Systems Design and Engineering)  9 （ 1 ） 56 - 62 2023年11月

  　概要を見る

  The separation capacity of a column typically remains constant. By applying stimuli-responsive materials to the stationary phase, the separation capacity in a single column can be tuned; however, the separation mode is not completely switched. In this study, we aimed to develop a cation/anion-exchange mode switching chromatography approach, in which the monomer ratio is adjusted, enabling the surface charge to become either negative or positive in response to mobile phase pH. Three types of beads were prepared, each modified with a pH-responsive mixed-charge polymer combining a cationic monomer, a pH-responsive carboxylic acid monomer, a neutral monomer, and a cross-linking monomer. The composition ratio of the cationic monomer to the pH-responsive carboxylic acid monomer was set at 1 : 2 so that the cation-exchange mode occurs at a pH above the pKa and the anion-exchange mode occurs below the pKa. At a pH below the pKa, the retention factor of the negatively charged compound increased. In contrast, at a pH above the pKa, the retention factor of the positively charged compound increased, confirming the charge switching on the bead surface. Switching to the cation- and anion-exchange mode enabled the separation of five basic antidepressants and acidic non-steroidal anti-inflammatory drugs, respectively. Utilizing a pH-responsive mixed-charge polymer, we attributed a cation/anion-exchange mode to a single column.

  • Access to Document (DOI)

• Dual Colorimetric/Electrochemical Detection of Salmonella typhimurium Using a Laser-Induced Graphene Integrated Lateral Flow Immunoassay Strip

  Preechakasedkit P., Panphut W., Lomae A., Wonsawat W., Citterio D., Ruecha N.

  Analytical Chemistry （Analytical Chemistry)  95 （ 37 ） 13904 - 13912 2023年09月

  ISSN  00032700

  　概要を見る

  Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to the millions of reported cases per year. It is essential to develop sensitive and rapid detection methods of foodborne pathogens to ensure food safety for producers and consumers. Unfortunately, current detection techniques still suffer from time-consuming operations and the need for highly skilled personnel. Here, we introduce a highly sensitive dual colorimetric/electrochemical detection approach for Salmonella enterica serovar typhimurium (S. typhimurium) based on a laser-induced graphene-integrated lateral flow immunoassay (LIG-LFIA) strip. The LIG electrode was fabricated by laser engraving on a polyimide tape containing a pseudo silver/silver chloride reference electrode from silver sintering and chlorination. Using double-sided tape inserted into the strip, automatic sequential reagent delivery was enabled for the dual-mode signal readout by single-sample loading. A gold-deposited gold nanoparticle strategy was first employed to simultaneously obtain a colorimetric signal for early screening and a signal turn-on electrochemical response for high-sensitivity and -quantitative analysis. A superior performance of the strip was established, characterized by a short analysis time (12 min assay +15 min sample preparation), a broad working concentration range (1 cfu/10 mL to 108 cfu/mL), and the lowest limit of detection (1 ± 0.5 cfu/10 mL; mean ± standard deviation, n = 3) among reported multimode S. typhimurium detection schemes. The strip was successfully applied in the analysis of various food products without any bacterial enrichment or amplification required, and the results were comparable to those of the standard culture method.

  • Access to Document (DOI)

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KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

KOARA（リポジトリ）収録論文等 【 表示 ／ 非表示 】

• Mgイオンはエネルギー代謝を制御する新規な細胞内情報伝達物質か？

  Daniel, Citterio

  科学研究費補助金研究成果報告書 2018年

• 細胞・臓器イメージング用新規化学センシング分子試薬の開発

  Citterio, Daniel

  科学研究費補助金研究成果報告書 2011年

総説・解説等 【 表示 ／ 非表示 】

総説・解説等 【 表示 ／ 非表示 】

• Editorial: Chemical Sensors for Biomedical Use

  Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

  Frontiers in Chemistry (Frontiers in Chemistry)  9 2021年05月

  • Access to Document (DOI)

• Equipment-free Detection of K⁺ on Paper

  Soda Y., Citterio D., Bakker E.

  Chimia (Chimia)  73 ( 11 )  2019年

  ISSN  00094293

  • Access to Document (DOI)

• Paper-based analytical devices

  Citterio D., Kaneta T.

  Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018年

  ISSN  09106340

  • Access to Document (DOI)

• Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

  チッテリオ　 ダニエル

  Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017年02月

  記事・総説・解説・論説等（学術雑誌）, 共著,  ISSN  1473-0197

  • Access to Document (DOI)

• 紙型マイクロ流体デバイス　検査チップの低コスト化と利便化を目指して

  山田　健太郎、チッテリオ　ダニエル

  化学と工業 (日本化学会)  69   120 - 122 2016年

  記事・総説・解説・論説等（その他）, 共著

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研究発表 【 表示 ／ 非表示 】

研究発表 【 表示 ／ 非表示 】

• Paper-Based Analytical Devices with CRISPR/Cas Signaling

  チッテリオ　ダニエル

  International Research Network on Microfluidic Analytical Technology (Bangkok) , 

  2024年04月

  , 

  口頭発表（招待・特別）, National Research Council of Thailand (NRCT)

• Paper-Based Analytical Devices with CRISPR/Cas Signal Amplification

  チッテリオ　ダニエル

  Pittcon 2024 (San Diego) , 

  2024年02月

  , 

  口頭発表（招待・特別）, The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy

• Low-cost and simple analytical devices suitable for point-of-care testing (POCT)

  チッテリオ　ダニエル

  The 16th Eurasia Conference on Chemical Sciences (Bangkok) , 

  2023年12月

  , 

  口頭発表（招待・特別）, The Chemical Society of Thailand

• Analytical Assays on Paper Platforms: As Simple as Possible

  チッテリオ　ダニエル

  Lab-on-a-Chip and Microfluidics Asia 2023 (Narita) , 

  2023年10月

  , 

  口頭発表（基調）, SelectBIO

• Clinical assays with paper, naked eye or camera: simplicity versus sensitivity?

  チッテリオ　ダニエル

  Euroanalysis 2023 (Geneva) , 

  2023年08月

  , 

  口頭発表（基調）,  Division of Analytical Chemistry of the European Chemical Society

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競争的研究費の研究課題 【 表示 ／ 非表示 】

競争的研究費の研究課題 【 表示 ／ 非表示 】

• CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

  2022年04月

  -

  2025年03月

  文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ ダニエル, 基盤研究(B), 補助金,  研究代表者

• 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

  2018年04月

  -

  2021年03月

  文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ,　ダニエル, 基盤研究(B), 補助金,  研究代表者

受賞 【 表示 ／ 非表示 】

受賞 【 表示 ／ 非表示 】

• 学術賞

  チッテリオ　ダニエル, 2022年03月, 日本化学会, 化学・生化学センシングのため機能性色素および紙基板分析デバイスの開発

  受賞区分： 国内学会・会議・シンポジウム等の賞

担当授業科目 【 表示 ／ 非表示 】

担当授業科目 【 表示 ／ 非表示 】

• 応用化学輪講

  2024年度

• 機器分析総論

  2024年度

• 応用化学系英語

  2024年度

• ナノスケール科学ジョイントセミナー

  2024年度

• マテリアルデザイン科学ジョイントセミナー

  2024年度

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担当経験のある授業科目 【 表示 ／ 非表示 】

担当経験のある授業科目 【 表示 ／ 非表示 】

• 化学・バイオセンサーとセンシングマテリアル

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  春学期, 講義, 専任

• 応用化学系英語

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  通年, 講義, 専任

• 基礎化学実験

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  秋学期, 実習・実験, 専任

• 応用化学実験

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  春学期, 実習・実験, 専任

• 機能物質概論

  慶應義塾

  2016年04月

  -

  2017年03月

  , 

  春学期, 講義, 専任

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所属学協会 【 表示 ／ 非表示 】

所属学協会 【 表示 ／ 非表示 】

• 日本化学会, 

  2006年04月

  -

  継続中

• 日本分析化学会, 

  2006年04月

  -

  継続中

• 米国化学会, 

  2007年03月

  -

  継続中

• 英国王立化学協会 (RSC) (フェロー), 

  2016年02月

  -

  継続中

委員歴 【 表示 ／ 非表示 】

委員歴 【 表示 ／ 非表示 】

• 2017年04月

  -

  継続中

  Permanent Steering Committee of Europt(r)ode, Europt(r)ode