チッテリオ, ダニエル (チッテリオ, ダニエル)

Citterio, Daniel

写真a

所属(所属キャンパス)

理工学部 応用化学科 (矢上)

職名

教授

HP

外部リンク

経歴 【 表示 / 非表示

  • 2002年11月
    -
    2003年09月

    スイス連邦工科大学, 化学センサーセンター, 上級研究員

  • 2005年02月
    -
    2006年01月

    チバ・スペシャルティ・ケミカルズ株式会社(スイス), 特許情報管理部, 特許弁理士

  • 2006年03月
    -
    2007年03月

    慶應義塾大学理工学部, 大学院理工学研究科, 特別研究助教授

  • 2007年04月
    -
    2009年03月

    慶應義塾大学理工学部, 応用化学科, 准教授(有期)

  • 2009年04月
    -
    2014年03月

    慶應義塾大学理工学部, 応用化学科, 准教授

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研究分野 【 表示 / 非表示

  • ナノテク・材料 / 分析化学

研究キーワード 【 表示 / 非表示

  • バイオセンサ

  • 化学センサ

  • 機能性色素

  • 機能材料

 

著書 【 表示 / 非表示

  • Applications of Microfluidic Systems in Biology and Medicine

    山田 健太郎、チッテリオ ダニエル, Springer, Singapore, 2019年04月

    担当範囲: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    チッテリオ  ダニエル, Springer, 2017年

    担当範囲: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    チッテリオ  ダニエル, Springer, 2015年

    担当範囲: Inkjet Printing of Biomolecules for Biorecognition / 197-235

論文 【 表示 / 非表示

  • Improvement in sensitivity for lateral flow immunoassay of ferritin using novel device design based on gold-enhanced gold nanoparticles

    Preechakasedkit P., Teekayupak K., Citterio D., Ruecha N.

    Scientific Reports (Scientific Reports)  12 ( 1 )  2022年12月

     概要を見る

    This work introduces a low-cost adhesive tape combined with a hydroxylamine/polyvinyl alcohol/polyethylene oxide (HA/PVA/PEO) blend film to fabricate novel devices for improving sensitivity of gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) via two platforms: (1) LFIA device with integrated gold enhancement and (2) LFIA device with two independent sample inlets. The detection of ferritin has been used for proof-of-concept. The adhesive tape inserted in the devices assists to separate two solutions independently flowing from two different inlets toward a nitrocellulose membrane. On-device gold enhancement was achieved by the enlargement of AuNPs via the catalytic reaction of KAuCl4 and HA using the HA/PVA/PEO blend film easily prepared via a solution-casting technique, which could delay the flow of HA released from the film for 180s and improve storage stability of the device. Under optimal conditions evaluated by naked eyes, the gold enhancement (LOD = 0.5 ng/mL) and double-sample inlet (LOD = 2 ng/mL) devices exhibited 20-fold and fivefold higher sensitivity respectively than a conventional device, verifying the sensitivity improvement. Furthermore, the proposed device was successfully detected ferritin in human serum samples within 10 min via naked-eye observation, exhibiting rapidity and simplicity of use, and the capability to perform on-site assays.

  • Quantitative evaluation of reversed-phase packing material based on calcium carbonate microspheres modified with an alternating copolymer

    Kaizu K., Mochida M., Imai H., Citterio D., Hiruta Y.

    Journal of Chromatography A (Journal of Chromatography A)  1677 2022年08月

    ISSN  00219673

     概要を見る

    Considering the vulnerability of silica gel to alkaline mobile phases, a highly alkaline stable stationary phase for HPLC is required to separate basic compounds with high separation efficiency. To address this issue, we have developed a high alkaline stable packing material (CaCO3-PMAcO) based on mesoporous calcium carbonate microspheres modified with poly(maleic acid-alt-1-octadecene). In this study, we report further investigation of the separation performance of CaCO3-PMAcO column by systematically evaluating the effects of particle size and chromatographic conditions. Based on the theory of the van Deemter equation, the separation efficiency was related to the size of CaCO3-PMAcO particles (2.9 – 5.7 µm). The evaluation of thermodynamics of retention by changing the column temperature from 20 °C to 45 °C implied that the retention mode was dominated by hydrophobic interaction associated with the exothermic enthalpy changes (-11.1 to -12.5 kJ/mol). The results of column selectivity tests revealed that the CaCO3-PMAcO column had hydrophobic selectivity comparable to C18 silica gel columns (αP/B; CaCO3-PMAcO column: 1.53, C18 column: 1.69), and higher shape/steric selectivity (αTri/Ter; CaCO3-PMAcO column: 1.56, C18 column: 0.955). In practice, the CaCO3-PMAcO column could be applied to the separation of not only alkylbenzenes and polycyclic aromatic hydrocarbons, but also to basic tricyclic antidepressants by using an alkaline mobile phase (pH 12).

  • Organelle-selective near-infrared fluorescent probes for intracellular microenvironment labeling

    Kurutos A., Shindo Y., Hiruta Y., Oka K., Citterio D.

    Dyes and Pigments (Dyes and Pigments)  204 2022年08月

    ISSN  01437208

     概要を見る

    The design, synthesis, and bio-applicability of a series of 8 near-infrared (NIR) molecular probes with tunable hydrophilic/hydrophobic properties are described in this paper. The title dyes comprise of symmetric heptamethine cyanines with various N-quaternary heterocyclic moieties including indole, benzo[e]indol, benzothiazole and naphtho[1,2-d]thiazole, along with an extra positive charge in close proximity to the polymethine backbone. Combined UV–Vis and spectrofluorimetric techniques in both organic and aqueous-buffered solutions were employed to investigate the optical properties of the NIR chromophores. Two of the fluorophores emerged remarkable fluorescence quantum yield (Φ = 0.083), while their brightness was found up to 3-fold that of the FDA approved Indocyanine green – ICG (Φ = 0.027). What is more, two of the dyes appear to outperform the later clinical benchmark fluorophore in terms of photostability. While the ICG was completely faded after 1 h of constant irradiation, nearly 70% of the original emission signal could be observed for one of the novel molecular probes. The log Pow values determined by a combination of HPLC and UV–Vis (partition between 1-octanol/water) cover a relatively large range, from −2.14 up to 7.92. Investigation on the cytotoxicity by means of the MTT assay revealed, that present dyes are suitable for bioanalytical purposes and are safe to use. This paper also includes fluorescence imaging and cellular applications, such as labeling of HeLa cervical cancer cells. The results from the confocal microscopy reveal the superior staining capability of current heptamethines since they show significantly brighter signal than the commercial ICG probe. An organelle-selective fluorescent staining was achieved depending on the dye chemical structure. Co-localization within the intracellular microenvironment (linear or granular - cytosol, mitochondria, lysosome, endoplasmatic reticulum or Golgi apparatus) was confirmed without interference, employing several conventional organelle dye trackers. To the best of our knowledge, heptamethine dyes represent the first example of a series NIR emitting probes, that exhibit rational structural modifications aiming to fine-tune the hydrophilic/hydrophobic properties furnishing organelle-targeted staining.

  • Ag<inf>3</inf>PO<inf>4</inf>/Ag nanocomposite for selective and sensitive cyanide determination in food samples through catalytical colorimetry using a paper-based test kit

    Malahom N., Jarujamrus P., Anutrasakda W., Siripinyanond A., Amatatongchai M., Citterio D., Nacapricha D.

    Sensors and Actuators B: Chemical (Sensors and Actuators B: Chemical)  356 2022年04月

    ISSN  09254005

     概要を見る

    A paper-based test kit for selective and sensitive determination of cyanide was successfully developed using a Ag3PO4/Ag nanocomposite for catalytic colorimetry. The Ag3PO4/Ag nanocomposite synthesized via a one-step chemical bath method could instantly oxidize the chromogenic substrate ABTS for the color development under acidic conditions without the requirement of H2O2. During the detection step, cyanide in the sample was converted into gaseous hydrogen cyanide (HCN) using sulfuric acid. The HCN species then reacted with the generated Ag+ species via coordinate covalent bonding to form silver cyanide complex ions [Ag (CN2)]-. The amount of Ag+ species used for oxidizing ABTS subsequently decreased and so did the color intensity on the paper-based test kit. Specifically, the color intensity decreased with increasing cyanide concentrations in the range of 2.50–20.0 mg L−1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.48 mg L−1 and 1.59 mg L−1, respectively. The developed test kit showed relatively high selectivity towards cyanide and high tolerance towards other cations and anions present in real samples. When applied to real samples including fruit juices and food products, the performance of the rapid and low-cost test kit was well validated by a conventional precipitation titration method.

  • A Series of Furimazine Derivatives for Sustained Live-Cell Bioluminescence Imaging and Application to the Monitoring of Myogenesis at the Single-Cell Level

    Orioka M., Eguchi M., Mizui Y., Ikeda Y., Sakama A., Li Q., Yoshimura H., Ozawa T., Citterio D., Hiruta Y.

    Bioconjugate Chemistry (Bioconjugate Chemistry)  33 ( 3 ) 496 - 504 2022年03月

    ISSN  10431802

     概要を見る

    Bioluminescence (BL) imaging, which utilizes light emitted through the enzymatic reaction of luciferase oxidizing its substrate luciferin, enables sensitive and noninvasive monitoring of life phenomena. Herein, we developed a series of caged furimazine (FMZ) derivatives by introducing a protective group at the C-3 position and a hydroxy group at the C-6 phenyl ring to realize long-term live-cell BL imaging based on the NanoLuc (NLuc)/NanoKAZ (NKAZ)-FMZ system. The membrane permeability and cytotoxicity of the substrates were evaluated and related to their hydrophobicity. Among the series, the derivative with the bulkiest protective group (adamantanecarbonyl group) and a hydroxy substituent (named Ad-FMZ-OH) showed significantly prolonged and constant BL signal in cells expressing NLuc compared to the native FMZ substrate. This derivative enabled continuous BL imaging at the single-cell level for 24 h. Furthermore, we applied Ad-FMZ-OH to BL imaging of myocyte fusion and succeeded in the consecutive and sensitive monitoring at a single-cell level over a day. In summary, NLuc/NKAZ-caged FMZ derivatives have the potential to be applied to live-cell BL imaging of various life phenomena that require long-term observation.

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KOARA(リポジトリ)収録論文等 【 表示 / 非表示

総説・解説等 【 表示 / 非表示

  • Editorial: Chemical Sensors for Biomedical Use

    Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

    Frontiers in Chemistry (Frontiers in Chemistry)  9 2021年05月

  • Equipment-free Detection of K<sup>+</sup> on Paper

    Soda Y., Citterio D., Bakker E.

    Chimia (Chimia)  73 ( 11 )  2019年

    ISSN  00094293

  • Paper-based analytical devices

    Citterio D., Kaneta T.

    Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018年

    ISSN  09106340

  • Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

    チッテリオ  ダニエル

    Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017年02月

    記事・総説・解説・論説等(学術雑誌), 共著,  ISSN  1473-0197

  • 紙型マイクロ流体デバイス 検査チップの低コスト化と利便化を目指して

    山田 健太郎、チッテリオ ダニエル

    化学と工業 (日本化学会)  69   120 - 122 2016年

    記事・総説・解説・論説等(その他), 共著

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研究発表 【 表示 / 非表示

  • Paper- and Thread-Based Immunoassay Devices with Colorimetric Bioluminescence Signaling

    チッテリオ ダニエル

    Pittcon 2019 (フィラデルフィア(アメリカ)) , 

    2019年03月

    口頭発表(招待・特別), The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy

  • Capillary Flow-Driven Analytical Devices for Sample-in-Signal-Out Assaying of Clinically Relevant Analytes

    チッテリオ ダニエル

    Flow Analysis XIV (バンコク(タイ)) , 

    2018年12月

    口頭発表(基調), The Chemical Society of Thailand

  • Paper-Based Analytical Device for the Detection of Antibodies in Biological Samples

    チッテリオ ダニエル

    PERCH - CIC Congress X (パッタヤー(タイ)) , 

    2018年07月

    口頭発表(招待・特別), Department of Chemistry, Faculty of Science, Mahidol University

  • Paper-Based Devices for Antibody Detection in Whole Blood with Bioluminescent Sensing Proteins

    チッテリオ ダニエル

    第78回分析化学討論会 (山口大学) , 

    2018年05月

    口頭発表(招待・特別), 日本分析化学会

  • Paper-Based Devices for Antibody Detection in Whole Blood with Bioluminescent Sensing Proteins

    チッテリオ  ダニエル

    Europt(r)ode 2018 (Naples, Italy) , 

    2018年03月

    口頭発表(一般)

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競争的研究費の研究課題 【 表示 / 非表示

  • 血中抗体医薬品のPOC分析を可能にするマイクロ流体糸基板センサー

    2018年04月
    -
    2021年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, チッテリオ, ダニエル, 基盤研究(B), 補助金,  研究代表者

受賞 【 表示 / 非表示

  • 学術賞

    チッテリオ ダニエル, 2022年03月, 日本化学会, 化学・生化学センシングのため機能性色素および紙基板分析デバイスの開発

    受賞区分: 国内学会・会議・シンポジウム等の賞

 

担当授業科目 【 表示 / 非表示

  • 応用化学輪講

    2022年度

  • 機器分析総論

    2022年度

  • 応用化学系英語

    2022年度

  • ナノスケール科学ジョイントセミナー

    2022年度

  • マテリアルデザイン科学ジョイントセミナー

    2022年度

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担当経験のある授業科目 【 表示 / 非表示

  • 応用化学系英語

    慶應義塾

    2016年04月
    -
    2017年03月

    通年, 講義, 専任

  • 基礎化学実験

    慶應義塾

    2016年04月
    -
    2017年03月

    秋学期, 実習・実験, 専任

  • 応用化学実験

    慶應義塾

    2016年04月
    -
    2017年03月

    春学期, 実習・実験, 専任

  • 機能物質概論

    慶應義塾

    2016年04月
    -
    2017年03月

    春学期, 講義, 専任

  • 機器分析総論

    慶應義塾

    2016年04月
    -
    2017年03月

    春学期, 講義, 専任

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所属学協会 【 表示 / 非表示

  • 日本化学会, 

    2006年04月
    -
    継続中
  • 日本分析化学会, 

    2006年04月
    -
    継続中
  • 米国化学会, 

    2007年03月
    -
    継続中
  • 英国王立化学協会 (RSC) (フェロー), 

    2016年02月
    -
    継続中

委員歴 【 表示 / 非表示

  • 2017年04月
    -
    継続中

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode