Citterio, Daniel

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry (Yagami)

Position

Professor

Related Websites

External Links
Page Top ▲

Career 【 Display / hide 】

  • 2002.11
    -
    2003.09

    Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland, Centre for Chemical Sensors, Senior Research Scientist

  • 2005.02
    -
    2006.01

    Ciba Specialty Chemicals Inc., Basel (Switzerland), Scientific Information Department, Patent Attorney

  • 2006.03
    -
    2007.03

    Keio University Faculty of Science and Technology, Graduate School of Science and Technology, Special Research Associate Professor

  • 2007.04
    -
    2009.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor (non-tenured)

  • 2009.04
    -
    2014.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor

display all >>

Page Top ▲
 

Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Analytical chemistry

Page Top ▲

Research Keywords 【 Display / hide 】

  • biosensors

  • chemical sensors

  • functional dyes

  • functional materials

Page Top ▲
 

Books 【 Display / hide 】

  • Applications of Microfluidic Systems in Biology and Medicine

    Yanawut Manmana, Kentaro Yamada, Daniel Citterio, Springer Nature Singapore Pte Ltd., 2024

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 443-493

  • Applications of Microfluidic Systems in Biology and Medicine

    Yamada Kentaro, Citterio Daniel, Springer, Singapore, 2019.04

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    Citterio Daniel, Springer, 2017

    Scope: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    Citterio Daniel, Springer, 2015

    Scope: Inkjet Printing of Biomolecules for Biorecognition / 197-235

Page Top ▲

Papers 【 Display / hide 】

  • Ratiometric fluorometry on microfluidic paper-based analytical device for simultaneous glucose and cholesterol detection using MnFe-layered double hydroxides as peroxidase mimic

    Kitchawengkul N., Prakobkij A., Saenmuangchin R., Citterio D., Nacapricha D., Jarujamrus P.

    Sensors and Actuators B: Chemical 435 2025.07

    ISSN  09254005

    　View Summary

    A highly sensitive ratiometric fluorescence sensing system was developed for simultaneous glucose and total cholesterol (TC) detection in whole blood using MnFe-layered double hydroxides (MnFe-LDHs) as a peroxidase mimic, combined with an o-phenylenediamine (OPD) substrate and nitrogen-doped graphene quantum dots (N-GQDs). The detection platform, an X-shaped laminated microfluidic paper-based analytical device (XL-μPAD), was fabricated via laser printing and cutting. The MnFe-LDHs' large surface area and layered structure provide a high affinity for OPD, with a Michaelis–Menten constant (KM) of 0.0127 mmol L−1. Upon placing a drop of blood on the XL-μPAD sample pad, the enzymatic reactions of glucose and TC produce H2O2, which MnFe-LDHs convert to hydroxyl radicals (•OH). These radicals oxidize OPD into fluorescent 2,3-diamino phenazine (DAP) with emission at 560 nm. Meanwhile, the N-GQDs emit fluorescence at 415 nm, which is quenched by DAP through the inner filter effect (IFE) and dynamic quenching, enabling ratiometric sensing via the intensity ratio (I560/I415). As H2O2 levels increase, a visible green emission appears, correlating with glucose and TC levels. This XL-μPAD system demonstrates promising potential as a portable device for multiplex biomarker detection and diagnostic applications.

  • Origami Paper-Based Immunoassay Device with CRISPR/Cas12a Signal Amplification

    Suzuki H., Tong G., Nath P., Hiruta Y., Citterio D.

    ACS Sensors 10 ( 3 ) 1811 - 1821 2025.03

    　View Summary

    In clinical diagnosis, the determination of target proteins at low concentration levels is generally performed by immunoassays, such as the enzyme-linked immunosorbent assay (ELISA), which is a time-consuming process. To date, paper-based ELISA platforms enabling faster and less expensive analysis have been developed, but their important issue for clinical applications is the limited sensitivity compared to conventional ELISA. To address this challenge, this paper introduces a simple, rapid, and highly sensitive detection method for non-nucleic acid targets achieved by integrating the CRISPR/Cas12a system into paper-based ELISA. An origami-type paper-based device enabling simple assay operation has been designed, and the detection of targets on the paper substrates is based on observing the fluorescence signal induced by the CRISPR/Cas12a enzyme cleaving a probe single-stranded DNA (ssDNA) labeled with fluorophore and quencher (FQ reporter). To enhance sensitivity, antibodies labeled with a network of multiple DNA activating the CRISPR/Cas12a enzyme have been utilized as detection antibodies. As a result, the developed device successfully boosted the detection sensitivity for both human IgG and the hepatitis B virus surface antigen (HBsAg). In particular, the limit of detection (LOD) for HBsAg was estimated to be 12 pg/mL, representing over 10-fold higher sensitivity compared with commercially available HBsAg ELISA kits (LOD: 200 pg/mL). In addition, the fluorescence response toward porcine whole blood samples containing different HBsAg concentrations was also confirmed by capturing images with a smartphone, followed by quantitative data analysis. These results demonstrate the potential applicability of the proposed platform for clinical tests at the point of care.

  • Bioluminescence readout lateral flow immunoassay using nanobody targeting aflatoxin B1

    Takahashi S., Hiruta Y., Citterio D.

    Analyst 150 ( 8 ) 1563 - 1570 2025.03

    ISSN  00032654

    　View Summary

    Multiple signal detection methods are known for lateral flow immunoassays (LFIAs), with colorimetric approaches dominating the field. However, their limited sensitivity is a remaining challenge. Fluorescence-based signaling is regarded as a more sensitive method, but it comes at the cost of partial sacrifice of the user-friendliness of LFIAs due to the requirement of an excitation light source. In this context, bioluminescence providing an inherently high signal to noise ratio without the need of excitation light could be an attractive alternative. But only a few studies have demonstrated the application of bioluminescence signaling in LFIAs. This work aimed at the development of a simple bioluminescence-based LFIA for the detection of aflatoxin B1 (AFB1), used as a model target in a competitive LFIA format. Signal transduction was achieved by nanobody-nanoluciferase (Nluc) fusion proteins. These small-sized recombinant heavy-chain-only antibodies derived from the camelidae family directly linked with the Nluc enzyme produce high intensity glow-type bioluminescence in combination with the furimazine substrate. LFIA devices consisting of a sample pad, nitrocellulose membrane and absorbent pad with AFB1-BSA conjugate deposited at the test line on the nitrocellulose membrane, achieved an LOD of 0.26 ng mL−1 for aqueous AFB1 solutions pre-mixed with Nanobody-Nluc and bioluminescence emission observed on an imaging system. More user-friendly LFIA devices with integrated conjugate pad and pre-deposited Nanobody-Nluc provided clear AFB1 concentration-dependent bioluminescence signals with low background and enabled readout with a standard digital camera, resulting in an LOD of 1.12 ng mL−1. Finally, the LFIA strips have been applied in AFB1-spiked oat milk samples. The LOD of 4.09 ng mL−1 achieved in the real sample matrix is well below the maximum allowable residual concentration of AFB1 in the U.S. (20 ng mL−1).

  • Ratiometric Imaging for Quantification of Elevated Ca<sup>2+</sup> in Neurons Using Synthetic Low-Affinity Fluorescent Probe

    Kuronuma Y., Shindo Y., Kumada R., Sakama A., Citterio D., Oka K., Hiruta Y.

    ACS Chemical Neuroscience 16 ( 4 ) 649 - 658 2025.02

    　View Summary

    The availability of various calcium ion (Ca2+) fluorescent probes has contributed to revealing physiological events related to intracellular Ca2+. However, conventional probes face challenges for quantitatively and selectively visualizing high Ca2+ concentrations in cells induced by any stimuli, including biomolecules or electrical signal that disrupt Ca2+ homeostasis. In this report, we designed and synthesized a low-affinity ratiometric Ca2+ probe, KLCA-Fura, utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a ligand, for which we recently demonstrated the suitability as a new low-affinity ligand for Ca2+. KLCA-Fura showed a blue shift in excitation wavelength with increasing Ca2+ concentration based on the intramolecular charge transfer (ICT). Its affinity for Ca2+ is lower than commercially available conventional Ca2+ probes. Furthermore, the selectivity for Ca2+ and the fluorescence intensity were considered sufficient to accurately detect Ca2+. The corresponding acetoxymethyl ester, KLCA-FuraAM, was synthesized for intracellular imaging and applied to Ca2+ quantification in neurons. KLCA-FuraAM enabled quantitative ratiometric monitoring of the two-step Ca2+ concentration increase induced by glutamate stimulation. While this two-step response was not clearly observed with a commercially available low-affinity ratiometric Ca2+ probe, Fura-FF, KLCA-FuraAM has demonstrated the potential to quantitatively visualize the behavior of high Ca2+ concentrations. The ratiometric low-affinity Ca2+ probe, KLCA-Fura, is expected to be a powerful tool for discovering new functions of Ca2+ in neurons.

  • Present and future of smartphone-coupled chemiluminescence and electrochemiluminescence assays: a mini-review

    Dutta C., Citterio D., Nath P.

    Analyst 150 ( 6 ) 1033 - 1047 2025.02

    ISSN  00032654

    　View Summary

    The convergence of smartphones with chemiluminescence and electrochemiluminescence (CL/ECL) assays marks a transformative leap in the realm of sensing technologies. The traditional CL/ECL assays, known for their high sensitivity and versatility, find extensive applications in medical diagnostics, environmental monitoring, food safety, and forensic sciences. However, these techniques have long been constrained due to the requirement of expensive instrumentation and complex reagent handling and hence their accessibility within certain environments is limited. In an era where rapid, accurate, and routine analysis is critical, smartphone-enabled CL/ECL systems offer substantial advantages over conventional analytical methods. By leveraging the universal accessibility and technological sophistication of smartphones and combining them with CL/ECL-based sensing, the smartphone has evolved into a cost-efficient and accessible analytical platform. The ability of the combined platform to conduct on-site analysis in real-time with minimal effort has emerged as a game-changer, particularly in low-resource settings. This mini-review explores the rapid evolution of smartphone-coupled CL/ECL systems over the last five years. The article covers the areas where the combined platform has been implemented in recent years for various sensing applications. The review further identifies key challenges that are associated with such combined platforms and finally highlights the future perspectives of the present topic.

display all >>

Page Top ▲

Papers, etc., Registered in KOARA 【 Display / hide 】

Page Top ▲

Reviews, Commentaries, etc. 【 Display / hide 】

display all >>

Page Top ▲

Presentations 【 Display / hide 】

  • Paper-Based Analytical Devices for Point-of-Care Testing: Can they be User-Friendly and Highly Sensitive?

    Daniel Citterio

    The 105th CSJ Annual Meeting (2025) (Osaka) , 

    2025.03
    , 

    Oral presentation (invited, special), The Chemical Society of Japan

  • CRISPR/Cas for highly sensitive paper-based analytical assays

    Daniel Citterio

    Pure and Applied Chemistry International Conference PACCON (Khao Yai) , 

    2025.02
    , 

    Oral presentation (keynote), Chemical Society of Thailand

  • CRISPR/Cas assays fully integrated into paper-based platforms

    Daniel Citterio

    Lab-on-a-Chip & Microfluidics Asia 2024 (Narita) , 

    2024.11
    , 

    Oral presentation (invited, special), SelectBIO

  • CRISPR/Cas Assays Integrated into Paper-Based Analytical Devices

    Daniel Citterio

    Matrafured International Conference on Chemical Sensors 2024 (Visegrad) , 

    2024.06
    , 

    Oral presentation (invited, special)

  • Paper-Based Analytical Devices Relying on CRISPR/Cas Signaling

    Daniel Citterio

    International Symposium on Science and Education (Taipei) , 

    2024.06
    , 

    Oral presentation (invited, special), National Taiwan Normal University

display all >>

Page Top ▲

Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

Page Top ▲

Awards 【 Display / hide 】

  • Award for Creative Work

    Daniel Citterio, 2022.03, The Chemical Society of Japan, Development of Functional Dyes and Paper-Based Analytical Devices for Chemical and Biochemical Sensing

    Type of Award： Award from Japanese society, conference, symposium, etc.

Page Top ▲
 

Courses Taught 【 Display / hide 】

  • ANALYTICAL CHEMISTRY

    2025

  • SEMINAR IN APPLIED CHEMISTRY

    2025

  • PRACTICAL INSTRUMENTAL ANALYSIS

    2025

  • PRACTICAL ENGLISH FOR APPLIED CHEMISTRY

    2025

  • NANO SCALE SCIENCE JOINT SEMINAR

    2025

display all >>

Page Top ▲

Courses Previously Taught 【 Display / hide 】

  • Chemical Sensors / Biosensors and Sensing Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

  • Practical English for Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Full academic year, Lecture, Within own ｆaculty

  • Laboratories in Basic Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Autumn Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Laboratories in Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Introduction to Functional Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

display all >>

Page Top ▲
 

Memberships in Academic Societies 【 Display / hide 】

  • Chemical Society of Japan (CSJ), 

    2006.04
    -
    Present

  • Japan Society for Analytical Chemistry (JSAC), 

    2006.04
    -
    Present

  • American Chemical Society (ACS), 

    2007.03
    -
    Present

  • Royal Society of Chemistry (RSC) (Fellow), 

    2016.02
    -
    Present
Page Top ▲

Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    Present

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode

Page Top ▲