Citterio, Daniel

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry (Yagami)

Position

Professor

Related Websites

External Links

Career 【 Display / hide

  • 2002.11
    -
    2003.09

    Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland, Centre for Chemical Sensors, Senior Research Scientist

  • 2005.02
    -
    2006.01

    Ciba Specialty Chemicals Inc., Basel (Switzerland), Scientific Information Department, Patent Attorney

  • 2006.03
    -
    2007.03

    Keio University Faculty of Science and Technology, Graduate School of Science and Technology, Special Research Associate Professor

  • 2007.04
    -
    2009.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor (non-tenured)

  • 2009.04
    -
    2014.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor

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Research Areas 【 Display / hide

  • Nanotechnology/Materials / Analytical chemistry

Research Keywords 【 Display / hide

  • biosensors

  • chemical sensors

  • functional dyes

  • functional materials

 

Books 【 Display / hide

  • Applications of Microfluidic Systems in Biology and Medicine

    Yamada Kentaro, Citterio Daniel, Springer, Singapore, 2019.04

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    Citterio Daniel, Springer, 2017

    Scope: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    Citterio Daniel, Springer, 2015

    Scope: Inkjet Printing of Biomolecules for Biorecognition / 197-235

Papers 【 Display / hide

  • Development of prediction model for cloud point of thermo-responsive polymers by experiment-oriented materials informatics

    Hayakawa M., Sakano K., Kumada R., Tobita H., Igarashi Y., Citterio D., Oaki Y., Hiruta Y.

    Polymer Chemistry (Polymer Chemistry)  14 ( 19 ) 2383 - 2389 2023.04

    ISSN  17599954

     View Summary

    Thermo-responsive polymers having a lower critical solution temperature (LCST) have attracted attention for biological applications such as drug delivery, diagnosis, and coating materials. In recent years, research on predicting LCST by utilizing machine learning has been conducted. However, since these methods targeted only copolymers combining specific monomer structures, they are not versatile, and multiple trials are still required to obtain new thermo-responsive polymers with the desired LCST. In this study, a prediction model for cloud point temperature (TCP) was built by a combination of materials informatics and chemical insight, named sparse modeling for small data (SpM-S) using a small dataset of polymers collected from the literature as training data. This approach created a model that is interpretable, easy to calculate, and versatile. The prediction accuracy was validated using data from different literature sources and experimental test data. The model was able to predict the TCP of polymers containing monomers not included in the dataset as well as polymers containing monomers included in the dataset. The predictive model has the potential to guide the design of new thermo-responsive polymers, and to contribute to efficient development of thermo-responsive polymers.

  • Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring

    Yoshii T., Nakano K., Okuda T., Citterio D., Hiruta Y.

    Analytical Methods (Analytical Methods)  15 ( 14 ) 1790 - 1796 2023.03

    ISSN  17599660

     View Summary

    Eggshell-based reversed-phase packing materials were applied to an analytical column for high-performance liquid chromatography. Commercially available eggshell powder was classified by a cyclone system to obtain three types of particles with different diameters (arithmetic mean ± standard deviation: 4.3 ± 3.8, 5.6 ± 3.3, and 9.5 ± 5.5 μm). Sedimentation separation removed tiny particles from each sample, resulting in particles with arithmetic means of 6.6 ± 5.5, 7.3 ± 4.5, and 10.2 ± 5.0 μm, respectively. The unclassified particles and three particle types treated with sedimentation separation were subsequently packed into analytical columns (150 mm × 4.6 mm I.D.), and their separation efficiencies were evaluated by comparing their height equivalent to a theoretical plate (HETP). The column without sedimentation separation exhibited the highest HETP, whereas the columns with sedimentation separation showed better separation efficiency and lower back pressure. The column with the best separation efficiency was applied for the separation of 10 alkylbenzenes and 5 steroids, and all peaks were observed with complete separation (peak resolution: RS > 1.5). Finally, the column was used for quantitative analysis of voriconazole, an azole antifungal agent, and imatinib, a first-generation molecularly targeted drug for cancer treatment, in spiked whole blood. Excellent accuracy (99.1-102.8%) and precision (0.6-1.9%) were observed for the spiked drugs and long-term stability (>3000 column volumes of mobile phase flow) indicated good applicability of the developed eggshell-based column as an analytical column for routine analyses of therapeutic drugs in blood.

  • Evaluation of cellophane as platform for colorimetric assays on microfluidic analytical devices

    Shigemori H., Maejima K., Shibata H., Hiruta Y., Citterio D.

    Microchimica Acta (Microchimica Acta)  190 ( 2 )  2023.02

    ISSN  00263672

     View Summary

    Due to their low cost, simplicity, and pump-free liquid transport properties, colorimetric assays on paper spots and microfluidic paper-based analytical devices (µPADs) are regarded as useful tools for point-of-care testing (POCT). However, for certain types of colorimetric assays, the “non-transparent” and “white” characters of paper can be a disadvantage. In this work, the possibilities of using cellophane as an alternative platform for colorimetric assays have been investigated. Cellophane is a low cost and easy-to-handle transparent film made of regenerated cellulose. Owing to its hydrophilic character, cellophane-based microfluidic channels fabricated through a print–cut–laminate approach enabled pump-free liquid transport into multiple detection areas, similar to µPADs. In addition, the water absorption characteristics of cellophane allowed the stable immobilization of water-soluble colorimetric indicators without any surface modification or additional reagents. The transparency of cellophane provides possibilities for simple background coloring of the substrates, increasing the dynamic signal range for hue-based colorimetric assays, as demonstrated for two model assays targeting H2O2 (46-fold increase) and creatinine (3.6-fold increase). Finally, a turbidity detection-based protein assay was realized on black background cellophane spots. The lowest limits of detection achieved with the cellophane-based devices were calculated as 7 µM for H2O2, 2.7 mg dL−1 for creatinine, and 3.5 mg dL−1 for protein (human serum albumin). Graphical Abstract: [Figure not available: see fulltext.]

  • Fabrication process development and basic evaluation of eggshell-based column packing material for reversed-phase preparative separation

    Yoshii T., Sakama A., Kanamori K., Nakanishi K., Imai H., Citterio D., Hiruta Y.

    Journal of Chromatography A (Journal of Chromatography A)  1688 2023.01

    ISSN  00219673

     View Summary

    Purification of basic drugs in reversed-phase mode is often difficult, mainly due to adsorption of positively charged compounds to the silica gel-based stationary phase. Since this adsorption can be suppressed under alkaline condition, columns with alkali-resistance are required. In addition, compounds with acid-sensitive structures are sometimes degraded during separation on silica gel-based columns which exhibit acidity due to their surface structure. We prepared an alkali-resistant reversed-phase packing material, Eggshell-PMAcO based on eggshells modified with an amphiphilic copolymer, poly(maleic acid-alt-1-octadecene) (PMAcO). The height equivalent to a theoretical plate (HETP) of the Eggshell-PMAcO column was improved by surface treatment with ammonium acetate buffer (900 mM, pH = 3.7), which is an inexpensive reagent, and the retention behavior for hydrophobic compounds was compared to a typical ODS column based on silica gel, resulting in sufficient selectivity of the eggshell-based column for hydrophobic compounds, as indicated by the ratio of retention factors of pentylbenzene and butylbenzene (Eggshell-PMAcO column: 1.55, ODS column: 1.65). Column temperature-dependent retention behavior of naphthalene was investigated in the temperature range from 25 °C to 45 °C, followed by the calculation of thermodynamic parameters. There was little difference in the standard molar enthalpy (Eggshell-PMAcO: −19.6 kJ/mol, ODS: −21.7 kJ/mol). The absolute value of the standard free Gibbs energy for the Eggshell-PMAcO column was much smaller than that of the ODS column (Eggshell-PMAcO: −0.284 kJ/mol, ODS: −13.0 kJ/mol), indicating that the Eggshell-PMAcO column had a weaker retention strength for naphthalene than the ODS column mainly due to the large difference in the standard molar entropy (Eggshell-PMAcO: −64.9 J/mol K, ODS column: −29.2 J/mol K). The retention capacities for imipramine under neutral (water/methanol) and alkaline (0.1% triethylamine water/methanol) conditions were 0.2 mg and 5 mg, respectively, based on injection mass-dependent HETP, retention factor and symmetry factor. Finally, the prepared column was applied to the purification of a building block for nucleic acid drugs. This study demonstrated that reversed-phase columns, which can be fabricated from eggshells and an amphiphilic copolymer in an inexpensive and eco-friendly way, have the ability to purify basic compounds and acid-sensitive compounds.

  • Effect of the side chain composition of mixed-charge polymers on pH-selective cell–membrane interactions

    Nakamura Y., Nasu M., Shindo Y., Oka K., Citterio D., Hiruta Y.

    Polymer Journal (Polymer Journal)   2023

    ISSN  00323896

     View Summary

    Zwitterionic polymers are characterized by their antifouling ability due to their net-neutral charge and affinity for cancer cell surfaces. We developed six pH-responsive mixed-charge polymers and investigated the effect of their side chain hydrophobicity on their interaction with cancer cell membranes. The polymers were synthesized using [2-(methacryloyloxy)ethyl]trimethylammonium chloride, carboxylic acid monomers, and butyl methacrylate (BMA) as the neutral spacer moiety, and differences in hydrophobicity were created by altering the type of carboxylic acid monomer and ratio of the spacer. The surface charge of the polymers was designed to switch from net-neutral to positive in response to the weakly acidic tumor environment. This tuning occurred in a more cancer-like environment with the usage of a more hydrophobic anionic monomer and a higher ratio of BMA due to the weakening of electrostatic interactions. This trend was further confirmed by evaluation of the interaction between the polymers and heparin, an anionic polysaccharide that is used as a model for cancer cell surfaces. The affinity of the mixed-charge polymers for cells was then evaluated, and both the hydrophobicity and pH-responsive nature of these polymers led to pH-selective toxicity, which was assumed to be caused by the disruption of cell membrane integrity.

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Papers, etc., Registered in KOARA 【 Display / hide

Reviews, Commentaries, etc. 【 Display / hide

  • Editorial: Chemical Sensors for Biomedical Use

    Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

    Frontiers in Chemistry (Frontiers in Chemistry)  9 2021.05

  • Equipment-free Detection of K<sup>+</sup> on Paper

    Soda Y., Citterio D., Bakker E.

    Chimia (Chimia)  73 ( 11 )  2019

    ISSN  00094293

  • Paper-based analytical devices

    Citterio D., Kaneta T.

    Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018

    ISSN  09106340

  • Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

    Yamada Kentaro, Shibata Hiroyuki, Suzuki Koji, Citterio Daniel

    Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017.02

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work,  ISSN  1473-0197

  • Paper-Based Microfluidic Devices - Towards Low-Cost and Convenient Test Chips

    Yamada, Kentaro; Citterio, Daniel

    Chemistry & Chemical Industry (2016), 69, 120-122 (The Chemical Society of Japan)  69   120 - 122 2016

    Article, review, commentary, editorial, etc. (other), Joint Work

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Presentations 【 Display / hide

  • Paper-Based Analytical Devices (PADs) for On-site Applications

    Citterio Daniel

    Gordon Research Conference Physics and Chemistry of Microfluidics (Barga) , 

    2023.06

    Oral presentation (invited, special), Gordon Research Conference

  • Approaches towards Point-of-Need Applications of Ion-Selective Optodes

    Citterio Daniel

    Pittcon 2023 (Philadelphia, Pennsylvania) , 

    2023.03

    Oral presentation (invited, special), The Pittsburgh Conference

  • Paper-Based Analytical Devices: How Simple Can Analysis Be?

    Citterio Daniel

    The 4th Materials Research Society of Thailand International Conference (Ubon Ratchathani) , 

    2023.02
    -
    2023.03

    Oral presentation (keynote), Materials Research Society of Thailand

  • Traffic Light Type Paper-Based Analytical Device for Intuitive and Semi-Quantitative Naked-Eye Signal Readout

    Citterio Daniel

    Matrafured 2022 International Conference on Chemical Sensors (Visegrad) , 

    2022.06

    Oral presentation (invited, special)

  • Ionophore-Based Cation-Exchange Optodes for Practical Use: With Paper Platforms to Sample pH-Independent Measurements

    Citterio Daniel

    Pittcon 2020 (Chicago, Illinois) , 

    2020.03

    Oral presentation (invited, special), The Pittsburgh Conference

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

Awards 【 Display / hide

  • Award for Creative Work

    Daniel Citterio, 2022.03, The Chemical Society of Japan, Development of Functional Dyes and Paper-Based Analytical Devices for Chemical and Biochemical Sensing

    Type of Award: Award from Japanese society, conference, symposium, etc.

 

Courses Taught 【 Display / hide

  • SEMINAR IN APPLIED CHEMISTRY

    2023

  • PRACTICAL INSTRUMENTAL ANALYSIS

    2023

  • PRACTICAL ENGLISH FOR APPLIED CHEMISTRY

    2023

  • NANO SCALE SCIENCE JOINT SEMINAR

    2023

  • MATERIAL DESIGN SCIENCE JOINT SEMINAR

    2023

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Courses Previously Taught 【 Display / hide

  • Chemical Sensors / Biosensors and Sensing Materials

    Keio University

    2016.04
    -
    2017.03

    Spring Semester, Lecture, Within own faculty

  • Practical English for Applied Chemistry

    Keio University

    2016.04
    -
    2017.03

    Full academic year, Lecture, Within own faculty

  • Laboratories in Basic Chemistry

    Keio University

    2016.04
    -
    2017.03

    Autumn Semester, Laboratory work/practical work/exercise, Within own faculty

  • Laboratories in Applied Chemistry

    Keio University

    2016.04
    -
    2017.03

    Spring Semester, Laboratory work/practical work/exercise, Within own faculty

  • Introduction to Functional Materials

    Keio University

    2016.04
    -
    2017.03

    Spring Semester, Lecture, Within own faculty

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Memberships in Academic Societies 【 Display / hide

  • Chemical Society of Japan (CSJ), 

    2006.04
    -
    Present
  • Japan Society for Analytical Chemistry (JSAC), 

    2006.04
    -
    Present
  • American Chemical Society (ACS), 

    2007.03
    -
    Present
  • Royal Society of Chemistry (RSC) (Fellow), 

    2016.02
    -
    Present

Committee Experiences 【 Display / hide

  • 2017.04
    -
    Present

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode