Citterio, Daniel

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry （Yagami）

Position

Professor

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Career 【 Display / hide 】

  • 2002.11
    -
    2003.09

    Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland, Centre for Chemical Sensors, Senior Research Scientist

  • 2005.02
    -
    2006.01

    Ciba Specialty Chemicals Inc., Basel (Switzerland), Scientific Information Department, Patent Attorney

  • 2006.03
    -
    2007.03

    Keio University Faculty of Science and Technology, Graduate School of Science and Technology, Special Research Associate Professor

  • 2007.04
    -
    2009.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor (non-tenured)

  • 2009.04
    -
    2014.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Analytical chemistry

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Research Keywords 【 Display / hide 】

  • biosensors

  • chemical sensors

  • functional dyes

  • functional materials

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Books 【 Display / hide 】

  • Applications of Microfluidic Systems in Biology and Medicine

    Yamada Kentaro, Citterio Daniel, Springer, Singapore, 2019.04

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    Citterio Daniel, Springer, 2017

    Scope: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    Citterio Daniel, Springer, 2015

    Scope: Inkjet Printing of Biomolecules for Biorecognition / 197-235

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Papers 【 Display / hide 】

  • Improvement in sensitivity for lateral flow immunoassay of ferritin using novel device design based on gold-enhanced gold nanoparticles

    Preechakasedkit P., Teekayupak K., Citterio D., Ruecha N.

    Scientific Reports （Scientific Reports)  12 （ 1 ）  2022.12

    　View Summary

    This work introduces a low-cost adhesive tape combined with a hydroxylamine/polyvinyl alcohol/polyethylene oxide (HA/PVA/PEO) blend film to fabricate novel devices for improving sensitivity of gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) via two platforms: (1) LFIA device with integrated gold enhancement and (2) LFIA device with two independent sample inlets. The detection of ferritin has been used for proof-of-concept. The adhesive tape inserted in the devices assists to separate two solutions independently flowing from two different inlets toward a nitrocellulose membrane. On-device gold enhancement was achieved by the enlargement of AuNPs via the catalytic reaction of KAuCl4 and HA using the HA/PVA/PEO blend film easily prepared via a solution-casting technique, which could delay the flow of HA released from the film for 180s and improve storage stability of the device. Under optimal conditions evaluated by naked eyes, the gold enhancement (LOD = 0.5 ng/mL) and double-sample inlet (LOD = 2 ng/mL) devices exhibited 20-fold and fivefold higher sensitivity respectively than a conventional device, verifying the sensitivity improvement. Furthermore, the proposed device was successfully detected ferritin in human serum samples within 10 min via naked-eye observation, exhibiting rapidity and simplicity of use, and the capability to perform on-site assays.

  • Quantitative evaluation of reversed-phase packing material based on calcium carbonate microspheres modified with an alternating copolymer

    Kaizu K., Mochida M., Imai H., Citterio D., Hiruta Y.

    Journal of Chromatography A （Journal of Chromatography A)  1677 2022.08

    ISSN  00219673

    　View Summary

    Considering the vulnerability of silica gel to alkaline mobile phases, a highly alkaline stable stationary phase for HPLC is required to separate basic compounds with high separation efficiency. To address this issue, we have developed a high alkaline stable packing material (CaCO3-PMAcO) based on mesoporous calcium carbonate microspheres modified with poly(maleic acid-alt-1-octadecene). In this study, we report further investigation of the separation performance of CaCO3-PMAcO column by systematically evaluating the effects of particle size and chromatographic conditions. Based on the theory of the van Deemter equation, the separation efficiency was related to the size of CaCO3-PMAcO particles (2.9 – 5.7 µm). The evaluation of thermodynamics of retention by changing the column temperature from 20 °C to 45 °C implied that the retention mode was dominated by hydrophobic interaction associated with the exothermic enthalpy changes (-11.1 to -12.5 kJ/mol). The results of column selectivity tests revealed that the CaCO3-PMAcO column had hydrophobic selectivity comparable to C18 silica gel columns (αP/B; CaCO3-PMAcO column: 1.53, C18 column: 1.69), and higher shape/steric selectivity (αTri/Ter; CaCO3-PMAcO column: 1.56, C18 column: 0.955). In practice, the CaCO3-PMAcO column could be applied to the separation of not only alkylbenzenes and polycyclic aromatic hydrocarbons, but also to basic tricyclic antidepressants by using an alkaline mobile phase (pH 12).

  • Organelle-selective near-infrared fluorescent probes for intracellular microenvironment labeling

    Kurutos A., Shindo Y., Hiruta Y., Oka K., Citterio D.

    Dyes and Pigments （Dyes and Pigments)  204 2022.08

    ISSN  01437208

    　View Summary

    The design, synthesis, and bio-applicability of a series of 8 near-infrared (NIR) molecular probes with tunable hydrophilic/hydrophobic properties are described in this paper. The title dyes comprise of symmetric heptamethine cyanines with various N-quaternary heterocyclic moieties including indole, benzo[e]indol, benzothiazole and naphtho[1,2-d]thiazole, along with an extra positive charge in close proximity to the polymethine backbone. Combined UV–Vis and spectrofluorimetric techniques in both organic and aqueous-buffered solutions were employed to investigate the optical properties of the NIR chromophores. Two of the fluorophores emerged remarkable fluorescence quantum yield (Φ = 0.083), while their brightness was found up to 3-fold that of the FDA approved Indocyanine green – ICG (Φ = 0.027). What is more, two of the dyes appear to outperform the later clinical benchmark fluorophore in terms of photostability. While the ICG was completely faded after 1 h of constant irradiation, nearly 70% of the original emission signal could be observed for one of the novel molecular probes. The log Pow values determined by a combination of HPLC and UV–Vis (partition between 1-octanol/water) cover a relatively large range, from −2.14 up to 7.92. Investigation on the cytotoxicity by means of the MTT assay revealed, that present dyes are suitable for bioanalytical purposes and are safe to use. This paper also includes fluorescence imaging and cellular applications, such as labeling of HeLa cervical cancer cells. The results from the confocal microscopy reveal the superior staining capability of current heptamethines since they show significantly brighter signal than the commercial ICG probe. An organelle-selective fluorescent staining was achieved depending on the dye chemical structure. Co-localization within the intracellular microenvironment (linear or granular - cytosol, mitochondria, lysosome, endoplasmatic reticulum or Golgi apparatus) was confirmed without interference, employing several conventional organelle dye trackers. To the best of our knowledge, heptamethine dyes represent the first example of a series NIR emitting probes, that exhibit rational structural modifications aiming to fine-tune the hydrophilic/hydrophobic properties furnishing organelle-targeted staining.

  • Ag<inf>3</inf>PO<inf>4</inf>/Ag nanocomposite for selective and sensitive cyanide determination in food samples through catalytical colorimetry using a paper-based test kit

    Malahom N., Jarujamrus P., Anutrasakda W., Siripinyanond A., Amatatongchai M., Citterio D., Nacapricha D.

    Sensors and Actuators B: Chemical （Sensors and Actuators B: Chemical)  356 2022.04

    ISSN  09254005

    　View Summary

    A paper-based test kit for selective and sensitive determination of cyanide was successfully developed using a Ag3PO4/Ag nanocomposite for catalytic colorimetry. The Ag3PO4/Ag nanocomposite synthesized via a one-step chemical bath method could instantly oxidize the chromogenic substrate ABTS for the color development under acidic conditions without the requirement of H2O2. During the detection step, cyanide in the sample was converted into gaseous hydrogen cyanide (HCN) using sulfuric acid. The HCN species then reacted with the generated Ag+ species via coordinate covalent bonding to form silver cyanide complex ions [Ag (CN2)]-. The amount of Ag+ species used for oxidizing ABTS subsequently decreased and so did the color intensity on the paper-based test kit. Specifically, the color intensity decreased with increasing cyanide concentrations in the range of 2.50–20.0 mg L−1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.48 mg L−1 and 1.59 mg L−1, respectively. The developed test kit showed relatively high selectivity towards cyanide and high tolerance towards other cations and anions present in real samples. When applied to real samples including fruit juices and food products, the performance of the rapid and low-cost test kit was well validated by a conventional precipitation titration method.

  • A Series of Furimazine Derivatives for Sustained Live-Cell Bioluminescence Imaging and Application to the Monitoring of Myogenesis at the Single-Cell Level

    Orioka M., Eguchi M., Mizui Y., Ikeda Y., Sakama A., Li Q., Yoshimura H., Ozawa T., Citterio D., Hiruta Y.

    Bioconjugate Chemistry （Bioconjugate Chemistry)  33 （ 3 ） 496 - 504 2022.03

    ISSN  10431802

    　View Summary

    Bioluminescence (BL) imaging, which utilizes light emitted through the enzymatic reaction of luciferase oxidizing its substrate luciferin, enables sensitive and noninvasive monitoring of life phenomena. Herein, we developed a series of caged furimazine (FMZ) derivatives by introducing a protective group at the C-3 position and a hydroxy group at the C-6 phenyl ring to realize long-term live-cell BL imaging based on the NanoLuc (NLuc)/NanoKAZ (NKAZ)-FMZ system. The membrane permeability and cytotoxicity of the substrates were evaluated and related to their hydrophobicity. Among the series, the derivative with the bulkiest protective group (adamantanecarbonyl group) and a hydroxy substituent (named Ad-FMZ-OH) showed significantly prolonged and constant BL signal in cells expressing NLuc compared to the native FMZ substrate. This derivative enabled continuous BL imaging at the single-cell level for 24 h. Furthermore, we applied Ad-FMZ-OH to BL imaging of myocyte fusion and succeeded in the consecutive and sensitive monitoring at a single-cell level over a day. In summary, NLuc/NKAZ-caged FMZ derivatives have the potential to be applied to live-cell BL imaging of various life phenomena that require long-term observation.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • Editorial: Chemical Sensors for Biomedical Use

    Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

    Frontiers in Chemistry (Frontiers in Chemistry)  9 2021.05

  • Equipment-free Detection of K<sup>+</sup> on Paper

    Soda Y., Citterio D., Bakker E.

    Chimia (Chimia)  73 ( 11 )  2019

    ISSN  00094293

  • Paper-based analytical devices

    Citterio D., Kaneta T.

    Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018

    ISSN  09106340

  • Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

    Yamada Kentaro, Shibata Hiroyuki, Suzuki Koji, Citterio Daniel

    Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017.02

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work,  ISSN  1473-0197

  • Paper-Based Microfluidic Devices - Towards Low-Cost and Convenient Test Chips

    Yamada, Kentaro; Citterio, Daniel

    Chemistry & Chemical Industry (2016), 69, 120-122 (The Chemical Society of Japan)  69   120 - 122 2016

    Article, review, commentary, editorial, etc. (other), Joint Work

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Presentations 【 Display / hide 】

  • Paper- and Thread-Based Immunoassay Devices with Colorimetric Bioluminescence Signaling

    Citterio Daniel

    Pittcon 2019 (Philadelphia, USA) , 

    2019.03
    , 

    Oral presentation (invited, special), The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy

  • Capillary Flow-Driven Analytical Devices for Sample-in-Signal-Out Assaying of Clinically Relevant Analytes

    Citterio Daniel

    Flow Analysis XIV (Bangkok, Thailand) , 

    2018.12
    , 

    Oral presentation (keynote), The Chemical Society of Thailand

  • Paper-Based Analytical Device for the Detection of Antibodies in Biological Samples

    Citterio Daniel

    PERCH - CIC Congress X (Pattaya, Thailand) , 

    2018.07
    , 

    Oral presentation (invited, special), Department of Chemistry, Faculty of Science, Mahidol University

  • Paper-Based Devices for Antibody Detection in Whole Blood with Bioluminescent Sensing Proteins

    Citterio Daniel

    78th Discussions on Analytical Chemistry (Ube) , 

    2018.05
    , 

    Oral presentation (invited, special), Japan Society for Analytical Chemistry

  • Paper-Based Devices for Antibody Detection in Whole Blood with Bioluminescent Sensing Proteins

    Citterio Daniel

    Europt(r)ode 2018 (Naples, Italy) , 

    2018.03
    , 

    Oral presentation (general)

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

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Awards 【 Display / hide 】

  • Award for Creative Work

    Daniel Citterio, 2022.03, The Chemical Society of Japan, Development of Functional Dyes and Paper-Based Analytical Devices for Chemical and Biochemical Sensing

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • CHEMICAL SENSORS / BIOSENSORS AND SENSING MATERIALS

    2022

  • BACHELOR'S THESIS

    2022

  • ANALYTICAL CHEMISTRY 1

    2022

  • ANALYTICAL CHEMISTRY

    2022

  • ENVIRONMENTAL AND ANALYTICAL CHEMISTRY

    2022

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Courses Previously Taught 【 Display / hide 】

  • Chemical Sensors / Biosensors and Sensing Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

  • Practical English for Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Full academic year, Lecture, Within own ｆaculty

  • Laboratories in Basic Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Autumn Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Laboratories in Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Introduction to Functional Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

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Memberships in Academic Societies 【 Display / hide 】

  • Chemical Society of Japan (CSJ), 

    2006.04
    -
    Present

  • Japan Society for Analytical Chemistry (JSAC), 

    2006.04
    -
    Present

  • American Chemical Society (ACS), 

    2007.03
    -
    Present

  • Royal Society of Chemistry (RSC) (Fellow), 

    2016.02
    -
    Present
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Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    Present

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode

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