Citterio, Daniel

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry ( Yagami )

Position

Professor

Related Websites

External Links
Scopus Paper Info  
Paper Count: 215 Citation Count: 8748 h-index: 47

Click to view the Scopus page. The data was downloaded from Scopus API in June 21, 2026, via http://api.elsevier.com and http://www.scopus.com .

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Career 【 Display / hide 】

  • 2002.11
    -
    2003.09

    Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland, Centre for Chemical Sensors, Senior Research Scientist

  • 2005.02
    -
    2006.01

    Ciba Specialty Chemicals Inc., Basel (Switzerland), Scientific Information Department, Patent Attorney

  • 2006.03
    -
    2007.03

    Keio University Faculty of Science and Technology, Graduate School of Science and Technology, Special Research Associate Professor

  • 2007.04
    -
    2009.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor (non-tenured)

  • 2009.04
    -
    2014.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Analytical chemistry

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Research Keywords 【 Display / hide 】

  • biosensors

  • chemical sensors

  • functional dyes

  • functional materials

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Books 【 Display / hide 】

  • Applications of Microfluidic Systems in Biology and Medicine

    Yanawut Manmana, Kentaro Yamada, Daniel Citterio, Springer Nature Singapore Pte Ltd., 2024

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 443-493

  • Applications of Microfluidic Systems in Biology and Medicine

    Yamada Kentaro, Citterio Daniel, Springer, Singapore, 2019.04

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    Citterio Daniel, Springer, 2017

    Scope: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    Citterio Daniel, Springer, 2015

    Scope: Inkjet Printing of Biomolecules for Biorecognition / 197-235

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Papers 【 Display / hide 】

  • Portable colorimetric device for low-density lipoproteins detection using aptamer-enhanced NiMnFe layered double hydroxide nanozyme

    Malahom N., Prakobkij A., Kusonsong C., Phooplub K., Buranachai C., Kasornsuwan P., Wattanasin P., Chunta S., Citterio D., Jarujamrus P.

    Microchimica Acta 193 ( 5 )  2026.05

    ISSN  00263672

    　View Summary

    A portable colorimetric platform was developed for rapid, selective, and sensitive detection of cholesterol-enriched low-density lipoprotein (LDL-C) in plasma by exploiting the aptamer-enhanced catalytic activity of NiMnFe layered double hydroxides (NiMnFe-LDHs). Unlike conventional enzymatic colorimetric assays and ELISA, which are costly, time-consuming, and reliant on sophisticated instrumentation, this system enables direct image acquisition and automated analysis using a single-board computer, thereby reducing assay time, operational complexity, and user dependence. Functionalization of NiMnFe-LDHs with an LDL-specific aptamer improves nanomaterial dispersion through electrostatic repulsion, increasing exposure of catalytic active sites and enhancing hydroxyl radical (•OH) generation. In addition, π–π interactions and hydrogen bonding between the aptamer and 3,3′,5,5′-tetramethylbenzidine (TMB) improve substrate affinity, further strengthening the peroxidase-like activity of the NiMnFe-LDHs@aptamer system. Upon LDL-C binding, formation of an LDL-C/aptamer corona partially blocks the LDH surface, limiting access of TMB and H<inf>2</inf>O<inf>2</inf> to catalytic sites. This suppresses •OH generation and TMB oxidation, resulting in a concentration-dependent decrease in colorimetric signal. The portable device achieved a linear detection range of 0.05–1.25 mg dL⁻¹ with a detection limit of 0.04 mg dL⁻¹. Although compatible with 96-well plates, the system’s key advantage lies in its integrated imaging and processing workflow, enabling scalable, high-throughput, and decentralized LDL-C diagnostics with potential extension to other biomarkers.

  • Dual thermo-responsive pH-Sensitive amino acid-derived zwitterionic copolymers: Balancing electrostatic and hydrophilic interactions in LCST–UCST behavior

    Kira M., Citterio D., Hiruta Y.

    Polymer 352 2026.04

    ISSN  00323861

    　View Summary

    Thermo-responsive polymers are widely applied in various fields, including drug delivery and surface coating. Dual thermo-responsive polymers, which exhibit both lower and upper critical solution temperatures (LCST and UCST), enable notable temperature-dependent behaviors within a tunable range. However, the structure–property relationships governing dual temperature-responsiveness are still insufficiently understood, particularly for polymers including pH-sensitive zwitterionic groups. In this study, LCST–UCST polymers, P(MEO<inf>2</inf>MA<inf>x</inf>MEOMA<inf>y</inf>GAASt<inf>20</inf>), composed of mono/di(ethylene glycol) methyl ether methacrylate (MEOMA/MEO<inf>2</inf>MA) and a zwitterionic monomer derived from glutamic acid (GAASt), were designed. By varying the ethylene glycol (EG) side chain length, pH, and salts, it was investigated how the balance between hydrophilic and electrostatic interactions governs the thermo-responsive behavior of the copolymers. In 10 mM phosphate buffer (PB, pH 7), UCST behavior was observed with only P(MEOMA<inf>79</inf>GAASt<inf>21</inf>), likely because shorter EG chains increased the relative contribution of zwitterionic units. The UCST of this polymer increased with the addition of salt, unlike typical zwitterionic UCST-type polymers. Polymers containing MEO<inf>2</inf>MA exhibited LCST behavior in 10 mM PB (pH 7) containing 100 mM NaCl, and the LCST decreased with shorter EG chains and kosmotropic ions. Under acidic and basic conditions, the thermo-responsive transition was not observed. Furthermore, P(MEO<inf>2</inf>MA<inf>19</inf>MEOMA<inf>61</inf>GAASt<inf>20</inf>) exhibited LCST–UCST behavior induced by NaCl. The diameter of the polymer in solution varied with temperature: terpolymer chains below the LCST, the largest aggregates between the LCST and UCST, and small globules above the UCST. Our findings provide insight into the molecular design strategies for thermo-responsive polymers in bioapplications.

  • Coelenterazine Analog Library for Specific Imaging of Serum Albumins on Conventional and Paper Microplates

    Kim S.B., Kamiya G., Nihongi R., Kinugasa S., Furuta T., Kitada N., Citterio D., Maki S.A.

    Bioconjugate Chemistry 37 ( 3 ) 590 - 601 2026.03

    ISSN  10431802

    　View Summary

    Paper-based analytical devices (PADs) are promising platforms to achieve Point-of-Care Testing (POCT) due to their low cost, ease of operation, disposability, and simplicity of assaying. Inspired by the bioanalytical potential of marine luciferins as excellent optical indicators, we evaluated a panel of 30 coelenterazine (CTZ) analogs for the specific imaging of important serum proteins from various species. This panel included 20 novel CTZ analogs, recently developed by the authors and designated as the “G-series”. Remarkably, several G-series CTZ analogs are exclusively bright with specific serum albumins and collectively create unique intensity patterns for these proteins. These G-series CTZ indicators produced highly quantitative luminescence signals with distinct spectral profiles across both trace and physiological albumin concentrations. This luminescence was utilized to determine kinetic constants, identify reaction sites, and establish dose–response curves for the various albumins. Finally, the optimized imaging system was transitioned to paper microplates for a rapid, on-site assay of serum albumins. HSA and BSA deposits dried on paper microplates were successfully imaged with CTZ indicators, achieving high signal-to-background ratios. This platform offers a cost-effective and portable approach to the rapid assay of serum albumins from various animal sources.

  • Progress toward real-world diagnostic applications of microfluidic paper-based analytical devices (μPADs)

    Tanifuji Y., Citterio D.

    Lab on A Chip 26 ( 5 ) 1191 - 1218 2026.03

    ISSN  14730197

    　View Summary

    Since their first report in 2007, microfluidic paper-based analytical devices (μPADs) have continued to gain attention as promising tools for point-of-care diagnostics due to their low cost, portability, ease of operation, and design flexibility. This review summarizes and discusses recent advances in the field, mostly based on works published between 2017 and 2025, with a focus on progress and remaining challenges in bridging the gap between proof-of-concept demonstrations in academic laboratories and real-world implementation. Special emphasis is placed on devices validated with clinical samples and capable of true sample-in–answer-out operation. To comprehensively assess recent developments, nearly one hundred reported examples were analysed not only in terms of analytical figures of merit but also with respect to practical criteria such as real-sample testing, long-term storage stability, the need for off-device sample pretreatment, reagent handling complexity, time-control requirements, and the number of operation steps. In parallel, topics of ongoing academic interest are highlighted, including automated sequential reagent delivery, strategies for accelerating liquid flow, and robust signal readout methods going beyond purely qualitative approaches to enhance assay sensitivity, precision, rapidity, and instrument-free usability. Finally, the review introduces emerging analytical technologies newly integrated into μPAD platforms, such as surface-enhanced Raman scattering (SERS), bioluminescence, CRISPR-based assays, and machine learning-driven data interpretation, which further expand the analytical capabilities and scope of μPADs.

  • Integrating smartphone optical sensors with paper-based analytical devices: a future standalone platform for onsite detection and analysis

    Manmana Y., Chetry B., Dutta S., Nath P., Citterio D.

    Trac Trends in Analytical Chemistry 196 2026.03

    ISSN  01659936

    　View Summary

    The beginning of this century has witnessed the emergence of many disruptive technologies changing the ways of sensing target analytes. Among these, smartphone-enabled analytical devices (SEADs) have gained attention as a promising technology, particularly in resource-limited settings. SEADs are recognized for being portable, cost-effective, user-friendly, and reliable platforms for detecting a wide range of analytes. In parallel, paper-based analytical devices (PADs) primarily relying on colorimetric or fluorescence signal transduction, have also attracted significant research interest across various applications. This review highlights the potential of integrating these two emerging analytical platforms, a combination that could revolutionize future onsite sensing. An overview of SEADs and PADs is provided, with focus on recent developments in optical detection. Advantages and limitations of integrating smartphones with PADs in different scenarios are presented. Additionally, recent applications of smartphone-coupled PADs are presented. Finally, key challenges associated with merging these two technologies are discussed.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

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Presentations 【 Display / hide 】

  • Barcode Approaches for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

    Daniel Citterio

    [International presentation]  Pittcon 2026, 

    2026.03
    , 

    Oral presentation (invited, special), The Pittsburgh Conference

  • Paper-based analytical devices spanning the range from utmost simplicity to highest sensitivity

    Daniel Citterio

    [International presentation]  Pacifichem 2025 (Honolulu) , 

    2025.12
    , 

    Oral presentation (invited, special), American Chemical Society

  • CRISPR/Cas-Assisted Assays Integrated into Low-Cost Analytical Devices for Point-of-Need Applications

    Daniel Citterio

    [International presentation]  Pacifichem 2025 (Honolulu) , 

    2025.12
    , 

    Oral presentation (invited, special), American Chemical Society

  • Barcode Strategies for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

    Daniel Citterio

    [International presentation]  Hub of Knowledge in Chemical Sensor and Biosensor Technology International Conference 2025 (Bangkok) , 

    2025.11
    , 

    Oral presentation (invited, special)

  • 1D and 2D Barcode Strategies for Semiquantitative Signal Readout of Colorimetric Microfluidic Paper-Based Analytical Devices

    Daniel Citterio

    [International presentation]  Lab-on-a-Chip & Microfluidics Asia 2025 (Narita) , 

    2025.10
    , 

    Oral presentation (invited, special), SelectBIO

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 核酸抽出から検出までを統合した高感度簡易核酸分析3D印刷ピペットチップ型デバイス

    2025.04
    -
    2028.03

    基盤研究(B), Principal investigator

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

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Awards 【 Display / hide 】

  • Award for Creative Work

    Daniel Citterio, 2022.03, The Chemical Society of Japan, Development of Functional Dyes and Paper-Based Analytical Devices for Chemical and Biochemical Sensing

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • BACHELOR'S THESIS

    2026

  • PRACTICAL ENGLISH FOR APPLIED CHEMISTRY

    2026

  • FUNDAMENTAL ANALYTICAL CHEMISTRY

    2026

  • ADVANCED COURSE OF EMERGING SCIENCE A

    2026

  • SEMINAR IN EMERGING PHYSICO-CHEMISTRY 2

    2026

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Courses Previously Taught 【 Display / hide 】

  • Practical English for Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Full academic year, Lecture, Within own ｆaculty

  • Laboratories in Basic Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Autumn Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Laboratories in Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Introduction to Functional Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

  • Practical Instrumental Analysis

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

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Memberships in Academic Societies 【 Display / hide 】

  • Chemical Society of Japan (CSJ), 

    2006.04
    -
    Present

  • Japan Society for Analytical Chemistry (JSAC), 

    2006.04
    -
    Present

  • American Chemical Society (ACS), 

    2007.03
    -
    Present

  • Royal Society of Chemistry (RSC) (Fellow), 

    2016.02
    -
    Present
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Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    Present

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode

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