Citterio, Daniel

写真a

Affiliation

Faculty of Science and Technology, Department of Applied Chemistry （Yagami）

Position

Professor

Related Websites

External Links
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Career 【 Display / hide 】

  • 2002.11
    -
    2003.09

    Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland, Centre for Chemical Sensors, Senior Research Scientist

  • 2005.02
    -
    2006.01

    Ciba Specialty Chemicals Inc., Basel (Switzerland), Scientific Information Department, Patent Attorney

  • 2006.03
    -
    2007.03

    Keio University Faculty of Science and Technology, Graduate School of Science and Technology, Special Research Associate Professor

  • 2007.04
    -
    2009.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor (non-tenured)

  • 2009.04
    -
    2014.03

    Keio University Faculty of Science and Technology, Department of Applied Chemistry, Associate Professor

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Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Analytical chemistry

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Research Keywords 【 Display / hide 】

  • biosensors

  • chemical sensors

  • functional dyes

  • functional materials

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Books 【 Display / hide 】

  • Applications of Microfluidic Systems in Biology and Medicine

    Yamada Kentaro, Citterio Daniel, Springer, Singapore, 2019.04

    Scope: Paper-Based Microfluidics for Point-of-Care Medical Diagnostics / 353-382

  • Materials for Chemical Sensing

    Citterio Daniel, Springer, 2017

    Scope: (Bio)Chemical Sensors Based on Paper / 29-74

  • Design of Polymeric Platforms for Selective Biorecognition

    Citterio Daniel, Springer, 2015

    Scope: Inkjet Printing of Biomolecules for Biorecognition / 197-235

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Papers 【 Display / hide 】

  • Simplified capture, extraction, and amplification of cellular DNA from water samples

    Kawaguchi M., Aoki H., Kamo H., Miura K., Hiruta Y., Simizu S., Citterio D.

    Analytical Sciences （Analytical Sciences)  40 （ 3 ） 501 - 510 2024.03

    ISSN  09106340

    　View Summary

    DNA analysis in water samples is attracting attention in various fields. However, conventional methods for DNA analysis require a work-intensive and time-consuming sample pre-treatment. In this study, a simplified pre-treatment method for analyzing DNA in water samples was evaluated. The process consists of filtration, DNA extraction, and amplification, which can be achieved within a short time. In the filtration process, two types of filters, firstly a tissue paper (Kimwipe) and then a glass filter (GF/F), were used in sequence. The first large pore size filter enabled a reduction in filtration time by removing large particulate matter impurities present in river water matrix. Cells spiked into 1 L of river water were recovered at more than 90% within approximately 5 min filtration time. Also, DNA was extracted from the captured cells directly on the surface of the filter in only 5 min. Thus, DNA collection and extraction from a water sample can be completed within about 10 min. Furthermore, PCR amplification was performed directly from DNA-attached filter sections, which greatly reduced the number of required pre-treatment steps. Finally, we succeeded in establishing a simple and fast on-site pre-treatment system by using a hand-driven syringe filtration method. This pre-treatment system is expected to offer the possibility for the future establishment of a rapid and easy DNA analysis method applicable to various types of water samples. Graphical abstract: (Figure presented.)

  • Peroxidase-like Activity of Aptamer-Gold Nanoparticles for Selective and Sensitive Fluorescence Detection of Low-Density Lipoproteins

    Prakobkij A., Saenmuangchin R., Chunta S., Amatatongchai M., Citterio D., Jarujamrus P.

    ACS Applied Nano Materials （ACS Applied Nano Materials)   2024

    　View Summary

    Low-density lipoprotein cholesterol (LDL-C), commonly called “bad cholesterol”, is crucial to cardiovascular health. Increased LDL-C levels pose a substantial risk to human health. As a result, there is a demand for reliable, affordable, and highly sensitive analytical methods for LDL-C detection. Herein, a facile fluorometric aptasensor for LDL-C detection based on the aptamer-enhanced peroxidase-mimicking activity of gold nanoparticles (AuNPs) has been developed. AuNPs were functionalized with LDL-C-specific thiolated aptamer to enhance their intrinsic peroxidase-like activity, which could effectively catalyze the oxidation of o-phenylenediamine dihydrochloride (OPD) by H2O2 into the yellow-fluorescent product 2,3-diamino phenazine (DAP). The characterization studies confirmed that the presence of the aptamer enhances the affinity of AuNPs toward the OPD substrate, thereby leading to a marked increase in the peroxidase-mimicking activity. Moreover, after being functionalized with an aptamer, increased dispersibility and substrate affinity of AuNPs were achieved. Using LDL-C as a target analyte, under optimum conditions, a linear relationship between smartphone-recorded signal intensity and the logarithm of the analyte concentration was observed in the range of 0.05-1 mg dL-1 and the limit of detection was 0.0230 mg dL-1. The results agree with those obtained by a clinical laboratory method. This proposed method is readily deployable and a promising prototype for diverse diagnostic applications, particularly in biomarker detection within serum or plasma samples. It holds the potential to yield substantial advantages in point-of-care testing.

  • Development of Phosphinate Ligand-Based Low-Affinity Ca<sup>2+</sup> Fluorescent Probes and Application to Intracellular Ca<sup>2+</sup> Imaging

    Kumada R., Sakama A., Shindo Y., Kuronuma Y., Iwasawa N., Citterio D., Oka K., Hiruta Y.

    Analytical Chemistry （Analytical Chemistry)  95 （ 45 ） 16683 - 16691 2023.11

    ISSN  00032700

    　View Summary

    Divalent metal cations such as calcium ion (Ca2+) and magnesium ion (Mg2+) are indispensable to the regulation of various cellular activities. In this research, we developed the KLCA series utilizing o-aminophenol-N,N-diacetate-O-methylene-methylphosphinate (APDAP) as a target binding site, which was reported recently as a highly free Mg2+-selective ligand. KLCA-301 with orange fluorescence based on a rhodamine fluorophore and KLCA-501 with near-infrared (NIR) fluorescence based on a Si-rhodamine fluorophore were synthesized, intended for application to multicolor imaging. The evaluation of the fluorescence response to Ca2+ and Mg2+ of the KLCA series indicated the applicability as low-affinity Ca2+ probes. While KLCA-301 mainly localized in the cytosol in cultured rat hippocampal neurons, KLCA-501 localized to the cytosol and granular organelles in neurons. Comparison of the fluorescence response of KLCA-301 and the high-affinity Ca2+ probe Fluo-4 upon stimulation by glutamate in stained neurons revealed that KLCA-301 could reflect the secondary large rise of intracellular Ca2+, which Fluo-4 could not detect. In addition, KLCA-501 showed a fluorescence response similar to the low-affinity Ca2+ probe Fluo-5N upon stimulation by glutamate in stained neurons, concluding that KLCA-301 and KLCA-501 could be used as low-affinity Ca2+ probes. The KLCA series offers new options for low-affinity Ca2+ probes. Moreover, KLCA-501 achieved simultaneous visualization of the change in Ca2+ and ATP concentrations and also in mitochondrial inner membrane potential in neurons. KLCA-501 is expected to be a strong tool that enables simultaneous multicolor imaging of multiple targets and elucidation of their relationship in cells.

  • Cation/anion-exchange mode switching chromatography utilizing pH-responsive mixed charge polymer-modified silica beads

    Kaku T., Deura K., Yoshii T., Citterio D., Hiruta Y.

    Molecular Systems Design and Engineering （Molecular Systems Design and Engineering)  9 （ 1 ） 56 - 62 2023.11

    　View Summary

    The separation capacity of a column typically remains constant. By applying stimuli-responsive materials to the stationary phase, the separation capacity in a single column can be tuned; however, the separation mode is not completely switched. In this study, we aimed to develop a cation/anion-exchange mode switching chromatography approach, in which the monomer ratio is adjusted, enabling the surface charge to become either negative or positive in response to mobile phase pH. Three types of beads were prepared, each modified with a pH-responsive mixed-charge polymer combining a cationic monomer, a pH-responsive carboxylic acid monomer, a neutral monomer, and a cross-linking monomer. The composition ratio of the cationic monomer to the pH-responsive carboxylic acid monomer was set at 1 : 2 so that the cation-exchange mode occurs at a pH above the pKa and the anion-exchange mode occurs below the pKa. At a pH below the pKa, the retention factor of the negatively charged compound increased. In contrast, at a pH above the pKa, the retention factor of the positively charged compound increased, confirming the charge switching on the bead surface. Switching to the cation- and anion-exchange mode enabled the separation of five basic antidepressants and acidic non-steroidal anti-inflammatory drugs, respectively. Utilizing a pH-responsive mixed-charge polymer, we attributed a cation/anion-exchange mode to a single column.

  • Dual Colorimetric/Electrochemical Detection of Salmonella typhimurium Using a Laser-Induced Graphene Integrated Lateral Flow Immunoassay Strip

    Preechakasedkit P., Panphut W., Lomae A., Wonsawat W., Citterio D., Ruecha N.

    Analytical Chemistry （Analytical Chemistry)  95 （ 37 ） 13904 - 13912 2023.09

    ISSN  00032700

    　View Summary

    Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to the millions of reported cases per year. It is essential to develop sensitive and rapid detection methods of foodborne pathogens to ensure food safety for producers and consumers. Unfortunately, current detection techniques still suffer from time-consuming operations and the need for highly skilled personnel. Here, we introduce a highly sensitive dual colorimetric/electrochemical detection approach for Salmonella enterica serovar typhimurium (S. typhimurium) based on a laser-induced graphene-integrated lateral flow immunoassay (LIG-LFIA) strip. The LIG electrode was fabricated by laser engraving on a polyimide tape containing a pseudo silver/silver chloride reference electrode from silver sintering and chlorination. Using double-sided tape inserted into the strip, automatic sequential reagent delivery was enabled for the dual-mode signal readout by single-sample loading. A gold-deposited gold nanoparticle strategy was first employed to simultaneously obtain a colorimetric signal for early screening and a signal turn-on electrochemical response for high-sensitivity and -quantitative analysis. A superior performance of the strip was established, characterized by a short analysis time (12 min assay +15 min sample preparation), a broad working concentration range (1 cfu/10 mL to 108 cfu/mL), and the lowest limit of detection (1 ± 0.5 cfu/10 mL; mean ± standard deviation, n = 3) among reported multimode S. typhimurium detection schemes. The strip was successfully applied in the analysis of various food products without any bacterial enrichment or amplification required, and the results were comparable to those of the standard culture method.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • Editorial: Chemical Sensors for Biomedical Use

    Xie X., Citterio D., Chumbimuni-Torres K., Xue M., Wang X.

    Frontiers in Chemistry (Frontiers in Chemistry)  9 2021.05

  • Equipment-free Detection of K<sup>+</sup> on Paper

    Soda Y., Citterio D., Bakker E.

    Chimia (Chimia)  73 ( 11 )  2019

    ISSN  00094293

  • Paper-based analytical devices

    Citterio D., Kaneta T.

    Analytical Sciences (Analytical Sciences)  34 ( 1 ) 5 - 6 2018

    ISSN  09106340

  • Toward Practical Application of Paper-Based Microfluidics for Medical Diagnostics: State-of-the-Art and Challenges

    Yamada Kentaro, Shibata Hiroyuki, Suzuki Koji, Citterio Daniel

    Lab on a Chip (The Royal Society of Chemistry)  17 ( 7 ) 1206 - 1249 2017.02

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work,  ISSN  1473-0197

  • Paper-Based Microfluidic Devices - Towards Low-Cost and Convenient Test Chips

    Yamada, Kentaro; Citterio, Daniel

    Chemistry & Chemical Industry (2016), 69, 120-122 (The Chemical Society of Japan)  69   120 - 122 2016

    Article, review, commentary, editorial, etc. (other), Joint Work

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Presentations 【 Display / hide 】

  • Paper-Based Analytical Devices with CRISPR/Cas Signaling

    Daniel Citterio

    International Research Network on Microfluidic Analytical Technology (Bangkok) , 

    2024.04
    , 

    Oral presentation (invited, special), National Research Council of Thailand (NRCT)

  • Paper-Based Analytical Devices with CRISPR/Cas Signal Amplification

    Daniel Citterio

    Pittcon 2024 (San Diego) , 

    2024.02
    , 

    Oral presentation (invited, special), The Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy

  • Low-cost and simple analytical devices suitable for point-of-care testing (POCT)

    Daniel Citterio

    The 16th Eurasia Conference on Chemical Sciences (Bangkok) , 

    2023.12
    , 

    Oral presentation (invited, special), The Chemical Society of Thailand

  • Analytical Assays on Paper Platforms: As Simple as Possible

    Daniel Citterio

    Lab-on-a-Chip and Microfluidics Asia 2023 (Narita) , 

    2023.10
    , 

    Oral presentation (keynote), SelectBIO

  • Clinical assays with paper, naked eye or camera: simplicity versus sensitivity?

    Daniel Citterio

    Euroanalysis 2023 (Geneva) , 

    2023.08
    , 

    Oral presentation (keynote),  Division of Analytical Chemistry of the European Chemical Society

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • CRISPR/Casを用いた事前増幅不要で高感度核酸検出可能な紙基板分析デバイス

    2022.04
    -
    2025.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(B), Principal investigator

  • Microfluidic thread-based sensor for the detection of therapeutic antibodies in blood at point-of-care

    2018.04
    -
    2021.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Principal investigator

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Awards 【 Display / hide 】

  • Award for Creative Work

    Daniel Citterio, 2022.03, The Chemical Society of Japan, Development of Functional Dyes and Paper-Based Analytical Devices for Chemical and Biochemical Sensing

    Type of Award： Award from Japanese society, conference, symposium, etc.

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Courses Taught 【 Display / hide 】

  • SEMINAR IN APPLIED CHEMISTRY

    2024

  • PRACTICAL INSTRUMENTAL ANALYSIS

    2024

  • PRACTICAL ENGLISH FOR APPLIED CHEMISTRY

    2024

  • NANO SCALE SCIENCE JOINT SEMINAR

    2024

  • MATERIAL DESIGN SCIENCE JOINT SEMINAR

    2024

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Courses Previously Taught 【 Display / hide 】

  • Chemical Sensors / Biosensors and Sensing Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

  • Practical English for Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Full academic year, Lecture, Within own ｆaculty

  • Laboratories in Basic Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Autumn Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Laboratories in Applied Chemistry

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Within own ｆaculty

  • Introduction to Functional Materials

    Keio University

    2016.04
    -
    2017.03
    , 

    Spring Semester, Lecture, Within own ｆaculty

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Memberships in Academic Societies 【 Display / hide 】

  • Chemical Society of Japan (CSJ), 

    2006.04
    -
    Present

  • Japan Society for Analytical Chemistry (JSAC), 

    2006.04
    -
    Present

  • American Chemical Society (ACS), 

    2007.03
    -
    Present

  • Royal Society of Chemistry (RSC) (Fellow), 

    2016.02
    -
    Present
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Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    Present

    Permanent Steering Committee of Europt(r)ode, Europt(r)ode

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