KEIO RESEARCHERS INFORMATION SYSTEM

Profile Summary 【 Display / hide 】

Profile Summary 【 Display / hide 】

• Study on physical and chemical characteristics of atmospheric particulate matter (PM2.5) associated with health hazard

Career 【 Display / hide 】

Career 【 Display / hide 】

• 2020.04

  -

  Present

  理工学部応用化学科, 大学教授

• 2023.04

  -

  Present

  理工学研究科開放環境科学専攻環境エネルギー科学専修, 専修主任

• 2015.04

  -

  2020.03

  理工学部応用化学科, 大学准教授

• 2007.10

  -

  2008.09

  University of Wisconson-Madison, USA, Visiting Assistant Professor

• 2007.04

  -

  2015.03

  理工学部応用化学科, 大学専任講師

display all >>

Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 1999.04

  -

  2002.03

  Tokyo University of Agriculture and Technology, Graduate School, Division of Agricltural Sciences

  Graduate School, Completed, Doctoral course

• 1997.04

  -

  1999.03

  Tokyo Metropolitan University, Graduate School, Division of Natural Science, Department of Chemistry

  Graduate School, Completed, Master's course

• 1993.04

  -

  1997.03

  Tokyo Metropolitan University, Faculty of Science, Department of Chemistry

  University, Graduated

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• Doctor (Agricultural Science), Tokyo University of Agriculture and Technology, Coursework, 2002.03

Research Areas 【 Display / hide 】

Research Areas 【 Display / hide 】

• Environmental Science/Agriculture Science / Environmental dynamic analysis (Environmental Dynamic Analysis)

• Nanotechnology/Materials / Green sustainable chemistry and environmental chemistry (Environmental Chemistry)

• Natural Science / Space and planetary sciences (Earth Astrohemistry)

• Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Transport phenomena and unit operations

Research Keywords 【 Display / hide 】

Research Keywords 【 Display / hide 】

• aerosol, PM2.5, fine particulate matter, physical and chemical characteristics, health impacts

Books 【 Display / hide 】

Books 【 Display / hide 】

• 環境化学

  OKUDA TOMOAKI, 日本有機地球化学会, 2014

  Scope: 日本地球化学会（監修）地球・環境有機分子質量分析マニュアル2014，第１部第５章

• Aerosol Pollution in China

  OKUDA TOMOAKI, TANAKA SHIGERU, HE KEBIN, MA YONGLIANG, 京都大学学術出版会、京都, 2007

  Scope: 24-32

Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Estimation of particle size distributions in the atmosphere—analysis of Fe and Ca particles as the representative examples

  Youn H., Miki K., Iwata A., Okuda T.

  Asian Journal of Atmospheric Environment （Asian Journal of Atmospheric Environment)  17 （ 1 ）  2023.12

  ISSN  19766912

  　View Summary

  Atmospheric aerosols, including primary aerosols emitted directly into the atmosphere and secondary aerosols generated in the atmosphere from various chemically complex particles, cause a variety of environmental problems such as climate change, photochemical smog formation, and a decrease in incoming solar radiation. Therefore, it is important to understand the causes of aerosol particles and their impact on human society. In particular, particle size is an important indicator of lung penetration depth, aerosol transport, and optical properties. Hence, we mathematically estimated the airborne particle size distributions of each chemical component by collecting aerosol samples from the atmosphere using two types of cyclone samplers, large and small cyclone samplers. This study’s findings also suggest that calculated changes in particle size distribution can reflect changes in particle sources. The higher resolution of the continuous functions will enable the detection of the subtle changes in particle size distributions of each chemical component, which is helpful to understand the temporal changes in the chemical properties of the airborne aerosol particles.

  • Access to Document (DOI)

• Polycyclic aromatic hydrocarbons in urban particle matter exacerbate movement disorder after ischemic stroke via potentiation of neuroinflammation

  Tanaka M., Okuda T., Itoh K., Ishihara N., Oguro A., Fujii-Kuriyama Y., Nabetani Y., Yamamoto M., Vogel C.F.A., Ishihara Y.

  Particle and Fibre Toxicology （Particle and Fibre Toxicology)  20 （ 1 ）  2023.12

  　View Summary

  Background: A recent epidemiological study showed that air pollution is closely involved in the prognosis of ischemic stroke. We and others have reported that microglial activation in ischemic stroke plays an important role in neuronal damage. In this study, we investigated the effects of urban aerosol exposure on neuroinflammation and the prognosis of ischemic stroke using a mouse photothrombotic model. Results: When mice were intranasally exposed to CRM28, urban aerosols collected in Beijing, China, for 7 days, microglial activation was observed in the olfactory bulb and cerebral cortex. Mice exposed to CRM28 showed increased microglial activity and exacerbation of movement disorder after ischemic stroke induction. Administration of core particles stripped of attached chemicals from CRM28 by washing showed less microglial activation and suppression of movement disorder compared with CRM28-treated groups. CRM28 exposure did not affect the prognosis of ischemic stroke in null mice for aryl hydrocarbon receptor, a polycyclic aromatic hydrocarbon (PAH) receptor. Exposure to PM2.5 collected at Yokohama, Japan also exacerbated movement disorder after ischemic stroke. Conclusion: Particle matter in the air is involved in neuroinflammation and aggravation of the prognosis of ischemic stroke; furthermore, PAHs in the particle matter could be responsible for the prognosis exacerbation.

  • Access to Document (DOI)

• Optimization of the QuEChERS extraction method to determine Polycyclic Aromatic Hydrocarbons (PAHs) in powder aerosol particles collected by cyclone

  Jing W., Nakano K., Shen Z., Okuda T.

  Environmental Technology and Innovation （Environmental Technology and Innovation)  31 2023.08

  　View Summary

  Conventional methods for collecting and extracting aerosol particles are challenging in accurately characterizing particle-bound polycyclic aromatic hydrocarbons (PAHs) contamination and conducting cellular exposure studies. Therefore, developing direct aerosol sample collection and simple pretreatment analytical methods is necessary. This study describes the optimization of the QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction method for the determination of PAHs in powder-form environmental standard materials (SRM1944) and proves it is also effective for analyzing PAHs in powder aerosol particle samples collected by cyclone. In this study, we used an I-optimal design response surface method combined with a desirability function to investigate the extraction effect of PAHs under different combinations of QuEChERS extraction factors, including extraction solvent, salt, and clean-up reagent. The optimized conditions of the method were determined using the 2-factor interaction (2FI) model combined with boundary conditions, resulting in acetonitrile/dichloromethane (7:1, v/v), Na2SO4/NaCl (1:1, w/w), and primary and secondary amine (PSA) as extraction solvents, extraction salts, and clean-up reagent, respectively. The optimized method showed good recoveries of PAHs reaching 76%–118% and 85%–121% for SRM1944 and standard solution. The results for cyclone-collected ambient powder aerosol particles extracted by the optimized QuEChERS illustrated that this improved procedure has good PAHs extraction efficiency as the traditional Soxhlet method, while reducing hazardous organic solvents usage, experimental cost (per mg sample) by 97% and sample pretreatment time. Overall, this combination of “powder aerosol particle collection and modified QuEChERS extraction” provides new insight on aerosol sample collection, extraction, and in vitro/in vivo exposure studies.

  • Access to Document (DOI)

• Evaluation of separation performance for eggshell-based reversed-phase HPLC columns by controlling particle size and application in quantitative therapeutic drug monitoring

  Yoshii T., Nakano K., Okuda T., Citterio D., Hiruta Y.

  Analytical Methods （Analytical Methods)  15 （ 14 ） 1790 - 1796 2023.03

  ISSN  17599660

  　View Summary

  Eggshell-based reversed-phase packing materials were applied to an analytical column for high-performance liquid chromatography. Commercially available eggshell powder was classified by a cyclone system to obtain three types of particles with different diameters (arithmetic mean ± standard deviation: 4.3 ± 3.8, 5.6 ± 3.3, and 9.5 ± 5.5 μm). Sedimentation separation removed tiny particles from each sample, resulting in particles with arithmetic means of 6.6 ± 5.5, 7.3 ± 4.5, and 10.2 ± 5.0 μm, respectively. The unclassified particles and three particle types treated with sedimentation separation were subsequently packed into analytical columns (150 mm × 4.6 mm I.D.), and their separation efficiencies were evaluated by comparing their height equivalent to a theoretical plate (HETP). The column without sedimentation separation exhibited the highest HETP, whereas the columns with sedimentation separation showed better separation efficiency and lower back pressure. The column with the best separation efficiency was applied for the separation of 10 alkylbenzenes and 5 steroids, and all peaks were observed with complete separation (peak resolution: RS > 1.5). Finally, the column was used for quantitative analysis of voriconazole, an azole antifungal agent, and imatinib, a first-generation molecularly targeted drug for cancer treatment, in spiked whole blood. Excellent accuracy (99.1-102.8%) and precision (0.6-1.9%) were observed for the spiked drugs and long-term stability (>3000 column volumes of mobile phase flow) indicated good applicability of the developed eggshell-based column as an analytical column for routine analyses of therapeutic drugs in blood.

  • Access to Document (DOI)

• Chemokine expression in human 3-dimensional cultured epidermis exposed to PM2.5 collected by cyclonic separation

  Kono M., Okuda T., Ishihara N., Hagino H., Tani Y., Okochi H., Tokoro C., Takaishi M., Ikeda H., Ishihara Y.

  Toxicological Research （Toxicological Research)  39 （ 1 ） 1 - 13 2023.01

  ISSN  19768257

  　View Summary

  Fine particulate matter (PM2.5) exposure has a risk of inducing several health problems, especially in the respiratory tract. The skin is the largest organ of the human body and is therefore the primary target of PM2.5. In this study, we examined the effects of PM2.5 on the skin using a human 3-dimensional cultured epidermis model. PM2.5 was collected by cyclonic separation in Yokohama, Japan. Global analysis of 34 proteins released from the epidermis revealed that the chemokines, chemokine C-X-C motif ligand 1 (CXCL1) and interleukin 8 (IL-8), were significantly increased in response to PM2.5 exposure. These chemokines stimulated neutrophil chemotaxis in a C-X-C motif chemokine receptor 2-dependent manner. The oxidative stress and signal transducer and activator of transcription 3 pathways may be involved in the increased expression of CXCL1 and IL-8 in the human epidermis model. Interestingly, in the HaCaT human keratinocyte cell line, PM2.5 did not affect chemokine expression but did induce IL-6 expression, suggesting a different effect of PM2.5 between the epidermis model and HaCaT cells. Overall, PM2.5 could induce the epidermis to release chemokines, followed by neutrophil activation, which might cause an unregulated inflammatory reaction in the skin.

  • Access to Document (DOI)

display all >>

Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Social implementation of medical assistive devices to remove surgical smoke

  Okuda, Tomoaki

  学事振興資金研究成果実績報告書 （慶應義塾大学)  2021

• Development of medical assistive devices for surgical smoke reduction

  Okuda, Tomoaki

  学事振興資金研究成果実績報告書 （慶應義塾大学)  2020

• High-volume collection of aerosols in India and China using the originally developed cyclone sampler and evaluation of their health impacts

  Okuda, Tomoaki

  科学研究費補助金研究成果報告書 2019

• Elucidation of the electrostatic charging state of ambient aerosols and evaluation of its effect on deposition to body tissue

  Okuda, Tomoaki

  科学研究費補助金研究成果報告書 2019

• Evaluation of health impact of transboundary air pollution by an intensive observation of the concentrations of aerosol surface area and chemical constituents

  Okuda, Tomoaki

  学事振興資金研究成果実績報告書 （慶應義塾大学)  2017

display all >>

Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• 環境大気エアロゾルの帯電状態測定の現状と展望

  奥田 知明

  クリーンテクノロジー 27 ( 4 ) 37 - 40 2017.06

  Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

• 連載 エアロゾル基礎講座 ―計測― 6. 分級②：慣性運動や遠心力を用いた分級

  奥田知明, 榎原研正, 飯田健次郎

  エアロゾル研究 (日本エアロゾル学会)  32 ( 4 ) 276 - 288 2017.12

  Article, review, commentary, editorial, etc. (scientific journal), Joint Work

• 環境大気中PM2.5の比表面積の測定

  奥田知明

  クリーンテクノロジー (日本工業出版(株))  25 ( 6 ) 23 - 26 2015.06

  Article, review, commentary, editorial, etc. (scientific journal), Single Work

• 東アジアにおけるエアロゾルのフィールド観測および分析法の開発

  奥田知明

  大気環境学会誌 (（公社）大気環境学会)  50 ( 1 ) 1 - 8 2015.01

  Article, review, commentary, editorial, etc. (scientific journal), Single Work

• 我が国におけるPM2.5の現状

  OKUDA TOMOAKI

  航空環境研究 18   3 - 9 2014.04

  Article, review, commentary, editorial, etc. (scientific journal), Single Work

Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• PM2.5の物理化学特性の解明と曝露実験による生体有害性評価

  奥田知明

  第88回日本衛生学会 (東京工科大学蒲田キャンパス、東京都大田区) , 

  2018.03

  , 

  Oral presentation (invited, special), 日本衛生学会

• Development of measurement techniques of physical and chemical characteristics of atmospheric aerosols related to their adverse health effect

  Okuda, T.

  Frontiers of Atmospheric Aerosol Studies: Toward the Understanding of Their Health and Climatic Effects (Nagoya, Japan) , 

  2018.01

  , 

  Oral presentation (invited, special)

• Numerical simulation of a parallel plate particle separator for measurement of charging state of PM2.5

  Yonemichi, T., Fukagata, K., Fujioka, K., Okuda, T.

  9th JSME-KSME Thermal and Fluids Engineering Conference (Okinawa, Japan) , 

  2017.10

  , 

  Poster presentation, JSME

• PM2.5を集めて測る ～捕集と特性解明の技術～

  奥田知明

  第7回CSJ化学フェスタ (タワーホール船堀、東京都江戸川区) , 

  2017.10

  , 

  Oral presentation (invited, special), 日本化学会

• 黄砂・PM2.5の健康乳幼児の目・鼻・呼吸器症状への影響 - エコチル調査追加調査『黄砂と子どもの健康調査（パート２）』より -

  金谷久美子、亀田貴之、大久保綾乃、森育子、増本年男、黒沢洋一、大西一成、奥田知明、中山健夫

  第58回大気環境学会年会 (兵庫医療大学、兵庫県神戸市) , 

  2017.09

  , 

  Poster presentation, 大気環境学会

display all >>

Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• Development of a Health Effect Evaluation System for Particulate Matter Characteristics for the Central Asian Region

  2023.09

  -

  2027.03

  国際共同研究加速基金（海外連携研究）, Principal investigator

• Development and practical application of a new filter to promote collaboration between the chemical and biological research fields of aerosol toxicity assessment

  2022.06

  -

  2025.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, 挑戦的研究（萌芽）, Principal investigator

• Evaluation of the influence of charging state of atmospheric aerosol on particle deposition on human body and ground surface

  2020.04

  -

  2024.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A) , Principal investigator

• Multidisciplinary Analysis of Airborne Particle Surface Area as a New Indicator of Adverse Health Effect of PM2.5

  2018.06

  -

  2021.03

  MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Exploratory) , Principal investigator

• 独自型サイクロン装置により大量捕集されたPM2.5の化学組成と化学形態の簡便迅速非破壊分析法の開発

  2017.11

  -

  2018.10

  （公財）鉄鋼環境基金, 一般研究助成, Commissioned ｒesearch, Principal investigator

display all >>

Intellectual Property Rights, etc. 【 Display / hide 】

Intellectual Property Rights, etc. 【 Display / hide 】

• 微小粒子の捕集装置

  Date applied： 特願2017-132547  2017.07 

  Date announced： 特開2018-023970  2018.02 

  Patent, Joint

• 自動オープンクロマトグラフィー装置

  Date applied：   2007.07 

  Date announced：   2007.09 

  Date issued： 3135990 

  Utility model, Joint

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• 2015 Asian Young Aerosol Scientist Award

  Tomoaki OKUDA, 2015.06, The Asian Aerosol Research Assembly, Outstanding contribution to aerosol science and technology in Asia

  Type of Award： International academic award (Japan or overseas)

• 2023 Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology

  Tomoaki OKUDA, and 4 other members, 2023.04, Ministry of Education, Culture, Sports, Science and Technology , Contributions to the mass-gathering event under the COVID-19 pandemic

  Type of Award： International academic award (Japan or overseas)

• Best Paper Award, Japan Society for Atmospheric Environment

  Tomoaki OKUDA, Masanobu SAKAIDE, Ayumi IWATA, Motoi FUJIWARA, 2020.09, Japan Society for Atmospheric Environment, Investigation of the Characteristics of Particulate Matter Suspended in a Subway Station Platform

  Type of Award： Award from Japanese society, conference, symposium, etc.

• Incentive Award 2015, Japan Association of Aerosol Science and Technology

  Tomoaki OKUDA, 2015.06, Japan Association of Aerosol Science and Technology, Elucidation of physical and chemical characteristics of atmospheric aerosols related to their adverse health effect

• Creative Work Award for Young Scientists, Japan Society for Atmospheric Environment

  Tomoaki OKUDA, 2014.09, Japan Society for Atmospheric Environment, Outstanding Works for Field Study on Aerosols in East Asia

display all >>

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• SEMINAR IN APPLIED CHEMISTRY

  2024

• PROJECT FOR MULTIDISCIPLINARY AND INTERDISCIPLINARY KNOWLEDGE (SUSTAINABLE ENVIRONMENT)

  2024

• LABORATORIES IN APPLIED CHEMISTRY C

  2024

• INTRODUCTION OF ENVIRONMENTAL CHEMISTRY

  2024

• INDEPENDENT STUDY ON SCIENCE FOR OPEN AND ENVIRONMENTAL SYSTEMS

  2024

display all >>

Courses Previously Taught 【 Display / hide 】

Courses Previously Taught 【 Display / hide 】

• 自然科学実験（化学）

  Keio University

  2018.04

  -

  2019.03

  , 

  Spring Semester, Laboratory work/practical work/exercise, Within own ｆaculty

• 環境化学１

  Keio University

  2018.04

  -

  2019.03

  , 

  Spring Semester, Lecture

• 応用化学実験CD

  Keio University

  2018.04

  -

  2019.03

  , 

  Autumn Semester, Laboratory work/practical work/exercise, Within own ｆaculty

• 大気環境科学

  Keio University

  2018.04

  -

  2019.03

  , 

  Autumn Semester, Lecture

• 化学B

  Keio University

  2018.04

  -

  2019.03

  , 

  Autumn Semester, Lecture

display all >>

Memberships in Academic Societies 【 Display / hide 】

Memberships in Academic Societies 【 Display / hide 】

• The Chemical Society of Japan, 

  2015.06

  -

  Present

• Japan Association of Aerosol Science and Technology, 

  2006.04

  -

  Present

• The Japan Society for Analytical Chemistry, 

  2004.04

  -

  Present

• 日本大気化学会, 

  2002.04

  -

  Present

• Japan Society for Atmospheric Environment, 

  2002.04

  -

  Present

display all >>

Committee Experiences 【 Display / hide 】

Committee Experiences 【 Display / hide 】

• 2022.08

  -

  Present

  常任理事, 日本エアロゾル学会

• 2020.09

  -

  Present

  常任理事, 大気環境学会

• 2020.06

  -

  2021.03

  新型コロナウイルス対策専門家会議委員, 内閣府

• 2019.04

  -

  Present

  環境安全推進委員会委員長, 日本化学会

• 2019.04

  -

  2020.03

  PM2.5対策総合推進委員会委員, 環境省

display all >>