Miyata, Shogo

写真a

Affiliation

Faculty of Science and Technology, Department of Mechanical Engineering (Yagami)

Position

Associate Professor

E-mail Address

E-mail address

Related Websites

External Links

Career 【 Display / hide

  • 2003.10
    -
    2004.03

    東京大学大学院工学系研究科21世紀COEリサーチアシスタント

  • 2004.04
    -
    2005.03

    東京大学大学院工学系研究科助手

  • 2004.04
    -
    2011.03

    産業技術総合研究所招聘型客員研究員

  • 2005.04
    -
    2007.03

    九州工業大学大学院生命体工学研究科助手

  • 2006.10
    -
    2007.03

    北九州市立大学国際環境工学部非常勤講師

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Academic Background 【 Display / hide

  • 1999.03

    The University of Tokyo, Faculty of Engineering, 機械工学科

    University, Graduated

  • 2001.03

    The University of Tokyo, Graduate School, Division of Engineering, 機械工学専攻

    Graduate School, Completed, Master's course

  • 2004.03

    The University of Tokyo, Graduate School, Division of Engineering, 機械工学専攻

    Graduate School, Completed, Doctoral course

Academic Degrees 【 Display / hide

  • 博士(工学), The University of Tokyo, Coursework, 2004.03

 

Research Areas 【 Display / hide

  • Biomedical engineering/Biomaterial science and engineering (Biomedical Engineering/Biological Material Studies)

  • Materials/Mechanics of materials

Research Keywords 【 Display / hide

  • Biomechanics

  • Tissue engineering

  • Biophysical engineering

  • On-chip cell screening system

Research Themes 【 Display / hide

  • Development of Biocompatible Flexible Electrode using Conductive Polymer, 

    2013.04
    -
    Present

  • Response of human skin fibroblast to stretch in wound healing process using a novel three-dimensional culture model, 

    2012.12
    -
    Present

  • Fundamental study of platelet diagnosis by dielectrophoretic phenomena, 

    2012.04
    -
    2015.03

  • In vitro formation of the neural network using the response of cells to electrical environment, 

    2011.04
    -
    Present

  • Effect of UV/ozone surface modification on proliferation of embryonic stem cells, 

    2011.04
    -
    2014.03

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Proposed Theme of Joint Research 【 Display / hide

  • 細胞チップ(皮膚,神経,毛髪組織,など)を用いた対象薬品および物質のスクリーニングテスト

    Interested in joint research with industry (including private organizations, etc.),  Desired form: Funded Research

  • 細胞チップ(皮膚,脂肪,神経)による創薬スクリーニングデバイスの開発

    Interested in joint research with industry (including private organizations, etc.),  Desired form: Funded Research, Cooperative Research

 

Books 【 Display / hide

  • 技術予測レポート2023(上)健康寿命の延伸を目指す日本の技術編

    株式会社日本能率協会総合研究所, 2013.12

    Scope: 第3章 治療機器・再生医療

  • Tissue Regeneration - From Basic Biology to Clinical Application

    S. Miyata, INTECH, 2012.03

    Scope: pp.473-488

  • Biomaterials in Asia

    S. Miyata, K. Homma, T. Numano, T. Ushida, T. Tateishi, World Scientific Pub., 2009.01

    Scope: pp.482-493

  • 立石科学技術振興財団助成研究成果集

    MIYATA Shogo, 立石科学技術振興財団, 2009

  • 中谷電子計測技術振興財団年報

    MIYATA Shogo, 中谷電子計測技術振興財団, 2008.08

    Scope: pp.67-70

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Papers 【 Display / hide

  • Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration

    Imashiro C., Hirano M., Morikura T., Fukuma Y., Ohnuma K., Kurashina Y., Miyata S., Takemura K.

    Scientific Reports (Scientific Reports)  10 ( 1 )  2020.12

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2020, The Author(s). Proteinases that digest the extracellular matrix are usually used to harvest cells from culture vessels in a general culture process, which lowers the initial adhesion rate in regenerative medicine. Cell sheet engineering is one of the most important technologies in this field, especially for transplantation, because fabricated cell sheets have rich extracellular matrixes providing strong initial adhesion. Current cell sheet fabrication relies on temperature-responsive polymer-coated dishes. Cells are cultured on such specialized dishes and subjected to low temperature. Thus, we developed a simple but versatile cell sheet fabrication method using ubiquitous culture dishes/flasks without any coating or temperature modulation. Confluent mouse myoblasts (C2C12 cell line) were exposed to ultrasonic vibration from underneath and detached as cell sheets from entire culture surfaces. Because of the absence of low temperature, cell metabolism was statically increased compared with the conventional method. Furthermore, viability, morphology, protein expression, and mRNA expression were normal. These analyses indicated no side effects of ultrasonic vibration exposure. Therefore, this novel method may become the standard for cell sheet fabrication. Our method can be easily conducted following a general culture procedure with a typical dish/flask, making cell sheets more accessible to medical experts.

  • Fundamental study of decellularization method using cyclic application of high hydrostatic pressure

    Zemmyo D., Yamamoto M., Miyata S.

    Micromachines (Micromachines)  11 ( 11 )  2020.11

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Decellularized tissues are promising materials that mainly consist of extracellular matrices (ECMs) obtained by removing all cells from organs and tissues. High hydrostatic pressure (HHP) has been used for decellularization to remove cells physically from organs or tissues rather than by chemical methods. However, ultrahigh pressure induces denaturation of the ECM structure. In this study, we examined the effects of cyclic HHP at low and high pressures on the cell membrane structure to establish a novel decellularization method that enables decellularization without the denaturation of the ECM. A decellularization device using cyclic HHP (maximum pressure: 250 MPa, cycle number: 5) was developed. NB1RGB cell suspension was injected into a plastic bag to be subjected to cyclic HHP. After applying cyclic HHP, the amount of DNA inside the cells and the morphological changes of the cells were evaluated. As a result, the amount of DNA inside the cells decreased after the cyclic HHP compared to the static HHP. In addition, cyclic HHP was suggested to promote the destruction of the cell and nuclear membrane. In conclusion, it was revealed that the cell structure could be denatured and destroyed by cyclic HHP at a lower level than that of previous approaches.

  • Continuous ES/feeder cell-sorting device using dielectrophoresis and controlled fluid flow

    Takahashi Y., Miyata S.

    Micromachines (Micromachines)  11 ( 8 )  2020.08

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Pluripotent stem cells (PSCs) are considered as being an important cell source for regenerative medicine. The culture of PSCs usually requires a feeder cell layer or cell adhesive matrix coating such as Matrigel, laminin, and gelatin. Although a feeder-free culture using a matrix coating has been popular, the on-feeder culture is still an eective method for the fundamental study of regenerative medicine and stem cell biology. To culture PSCs on feeder cell layers, the elimination of feeder cells is required for biological or gene analysis and for cell passage. Therefore, a simple and cost-eective cell sorting technology is required. There are several commercialized cell-sorting methods, such as FACS or MACS. However, these methods require cell labeling by fluorescent dye or magnetic antibodies with complicated processes. To resolve these problems, we focused on dielectrophoresis (DEP) phenomena for cell separation because these do not require any fluorescent or magnetic dyes or antibodies. DEP imposes an electric force on living cells under a non-uniform AC electric field. The direction and magnitude of the DEP force depend on the electric property and size of the cell. Therefore, DEP is considered as a promising approach for sorting PSCs from feeder cells. In this study, we developed a simple continuous cell-sorting device using the DEP force and fluid-induced shear force. As a result, mouse embryonic stem cells (mESCs) were purified from a mixed-cell suspension containing mESCs and mouse embryonic fibroblasts (MEFs) using our DEP cell-sorting device.

  • Cost-effective culture of human induced pluripotent stem cells using UV/ozone-modified culture plastics with reduction of cell-adhesive matrix coating

    Kasai K., Tohyama S., Suzuki H., Tanosaki S., Fukuda K., Fujita J., Miyata S.

    Materials Science and Engineering C (Materials Science and Engineering C)  111 2020.06

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  09284931

     View Summary

    © 2020 Elsevier B.V. Human induced pluripotent stem cells (hiPSCs) are considered to be one of the most promising cell resources for regenerative medicine. HiPSCs usually maintain their pluripotency when they are cultured on feeder cell layers or are attached to a cell-adhesive extracellular matrix. In this study, we developed a culture system based on UV/ozone modification for conventional cell culture plastics to generate a suitable surface condition for hiPSCs. Time of flight secondary ion mass spectrometry (ToF-SIMS) was carried out to elucidate the relationship between hiPSC adhesion and UV/ozone irradiation-induced changes to surface chemistry of cell culture plastics. Cell culture plastics with modified surfaces enabled growth of a feeder-free hiPSC culture with markedly reduced cell-adhesive matrix coating. Our cell culture system using UV/ozone-modified cell culture plastics may produce clinically relevant hiPSCs at low costs, and can be easily scaled up in culture systems to produce a large number of hiPSCs.

  • Effect of mechanical compression on invasion process of malignant melanoma using in vitro three-dimensional cell culture device

    Morikura T., Miyata S.

    Micromachines (Micromachines)  10 ( 10 )  2019.10

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    © 2019 by the authors. Malignant melanoma in the plantar surface of the foot is subjected to various mechanical stimuli generated by daily human activity such as walking. Some studies have reported that mechanical compression affects the development and progression of melanoma. However, little is known about how mechanical compression affects the behavior of malignant melanoma cells in a physiological condition due to the complexity of the invasion mechanisms. In this study, we developed an in vitro three-dimensional cell culture device using microporous membrane in order to evaluate the effects of mechanical compression on the invasion process of malignant melanoma. Our results suggest that the invasion of melanoma cells under the compressive stress for 8 h of culture was promoted with the elongation of F-actin filaments compared to control groups, whereas there was no significant difference between both groups at 32 h of culture, with increasing cell death associated with promoting melanin synthesis. The results of this study contribute to the elucidation of the invasion mechanisms of malignant melanoma caused by mechanical stimulation.

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Papers, etc., Registered in KOARA 【 Display / hide

Reviews, Commentaries, etc. 【 Display / hide

  • Biocompatibility of materials : mechanical compatibility of biomaterials

    Shogo Miyata

    Pharm stage 16 ( 10 ) 53 - 57 2017.01

    Introduction and explanation (scientific journal), Single Work

  • Non-invasive assessment technology for tissue-engineered material

    Shogo Miyata

    Pharm stage 17 ( 5 )  2017

    Introduction and explanation (scientific journal), Single Work

  • 微粒子ピーニングによる細胞適合表面の創製とその応用

    小茂鳥 潤, 倉科佑太, 村井一恵, 宮田昌悟, 竹村研治郎, 小山尹誉

    砥粒加工学会誌 57 ( 6 ) 349 - 352 2013

    Introduction and explanation (scientific journal), Joint Work

Presentations 【 Display / hide

  • Effect of UV/ozone and atmospheric pressure plasma treatment of cell culture substrates on adhesion and proliferation of mouse ESCs

    Hayato Suzuki, Kohei Kasai, Yuka Kimura, Shogo Miyata

    The 17th Congress of the Japanese Society for Regenerative Medicine (横浜) , 2018.03, Poster (general)

  • In vitro invasion model of malignant melanoma and effect of mechanical stress on invasion process

    Takashi Morikura, Shogo Miyata

    JSME 30th Bioengineering Conference (名古屋) , 2017.12, Oral Presentation(general)

  • Effect of platelet rich plasma concentration on chondrogenesis of cartilage progenitor cells and response to compressive deformation stimuli

    Daiki Zenmyo, Yosikiyo Kibe, Shogo Miyata

    The 44th Annual Meeting of the Japanese Society for Clinical Biomechanics (奈良) , 2017.11

  • Reduction of cell-adhesive matrix coating for hiPSC culture using culture substrates treated with UV/ozone surface modification

    Shogo Miyata, Shugo Tohyama, Kohei Kasai, Jun Fujita, Kei-ichi Fukuda

    The 39th Annual Meeting of the Japanese Society for Biomaterials, 2017.11, Poster (general)

  • Evaluation system of adipocyte differentiation under three-dimensional culture condition using electrical impedance measurement

    Daiki Zenmyo, #HShogo Miyata<U/>

    JSME 28th Conference on Frontiers in Bioengineering (徳島) , 2017.10, Oral Presentation(general)

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • 細胞配置と力学的刺激の複合効果がもたらす毛髪および皮膚附属器官の完全生体外再生

    2017.04
    -
    2020.03

    Grant-in-Aid for Scientific Research, Research grant

  • プラズマ・ラジカル複合反応を用いた生体分子疑似構造を有するヒトiPS培養基材

    2017.04
    -
    2018.03

    文部科学省, 橋渡し研究加速ネットワークプログラム(シーズA), Research grant, Principal Investigator

  • プラズマ・ラジカル複合反応を用いた生体分子疑似構造を有するヒトiPS培養基材

    2016.12
    -
    2017.03

    文部科学省, 橋渡し研究加速ネットワークプログラム(シーズA), Research grant, Principal Investigator

  • 酸素ラジカル表面改質基材を用いたヒトiPS 細胞の完全単独培養システムと再生心筋細胞移植への展開

    2015.09
    -
    2016.03

    文部科学省, 橋渡し研究加速ネットワークプログラム(シーズA), Research grant

  • 幹細胞の多殻ビーズ封入技術と完全性体外での毛髪再生への新展開

    2014.04
    -
    2016.03

    Grant-in-Aid for Scientific Research, Research grant, Principal Investigator

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Works 【 Display / hide

  • 未来の起源

    MIYATA Shogo

    2013.04
    -
    Present

    Other, Single

  • ES細胞安定回収 フィーダ細胞分離 誰でも素早く

    S. Miyata

    2012.08
    -
    Present

    Other, Single

  • 骨や靱帯を再生させる技術って?

    MIYATA Shogo

    2011.07
    -
    Present

    Other, Single

  • 慶大、不良・良好な細胞傷つけずに分離する方法開発

    S. Miyata

    2011.07
    -
    Present

    Other, Single

  • 人のカラダは作ることができるか?

    MIYATA Shogo

    2010.08
    -
    Present

    Other, Single

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Intellectual Property Rights, etc. 【 Display / hide

  • 細胞担持用基材及びその製造方法

    Application No.: PCT/JP2016/000981  2016.02 

    Patent, Joint, PCT international application

  • 細胞担持用基材及びその製造方法

    Application No.: 特願2015-35439  2015.02 

    Patent, Joint

Awards 【 Display / hide

  • Best Paper Award

    Y. Kurashina, I. H. M. Hashim, K. Takemura, S. Miyata, J. Komotori, 2015.11, ASME, Resonance Vibration and Temperature Modulation Enhances Cell Detachment from Cultivation Substrate

    Type of Award: International Academic Awards.  Country: United States of America

  • 平成23年度日本材料学会 生体・医療材料部門 研究奨励賞

    MIYATA Shogo, 2012.03

    Type of Award: Awards of National Conference, Council and Symposium

  • 日本機械学会バイオエンジニアリング部門瀬口賞

    MIYATA Shogo, 2012.01, 日本機械学会, 再生軟骨および関節軟骨の非侵襲評価技術に関する研究

    Type of Award: Awards of National Conference, Council and Symposium

  • 2011年日本臨床バイオメカニクス学会学会奨励賞

    MIYATA Shogo, 2011.11, 誘電泳動チップを用いた軟骨細胞の分化・脱分化状態の識別

    Type of Award: Awards of National Conference, Council and Symposium

  • 平成22年度日本材料学会学術奨励賞

    MIYATA Shogo, 2011.05, 磁気共鳴イメージング(MRI)手法を用いた再生医療用材料の非侵襲評価法

    Type of Award: Awards of National Conference, Council and Symposium

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Courses Taught 【 Display / hide

  • GRADUATE RESEARCH ON INTEGRATED DESIGN ENGINEERING 2

    2020

  • GRADUATE RESEARCH ON INTEGRATED DESIGN ENGINEERING 1

    2020

  • FACTORY VISITING

    2020

  • EXPRESSION OF MECHANICAL PRODUCTS

    2020

  • BIOMATERIAL ENGINEERING

    2020

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Courses Previously Taught 【 Display / hide

  • 情報処理同実習

    慶應義塾大学理工学部, 2018

  • 図形情報処理

    慶應義塾大学理工学部, 2018

  • 機械工学実験

    慶應義塾大学理工学部, 2018

  • 機械工学創造演習

    慶應義塾大学理工学部, 2018

  • 形状情報の表現

    Keio University, 2017, Autumn Semester, Major subject, Laboratory work/practical work/exercise

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Memberships in Academic Societies 【 Display / hide

  • 日本機械学会, 

    2011.04
    -
    Present
  • 化学工学会, 

    2011.04
    -
    2013.03
  • 日本材料学会, 

    2009.04
    -
    Present
  • 日本バイオレオロジー学会, 

    2007
    -
    Present
  • American Society of Mechanical Engineers (ASME), 

    2005
    -
    Present

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Committee Experiences 【 Display / hide

  • 2012.04
    -
    2014.03

    運営委員, 日本機械学会 バイオエンジニアリング部門

  • 2011.04
    -
    2014.03

    関東支部代議員, 日本機械学会

  • 2011.04
    -
    2013.03

    編集委員, 化学工学誌

  • 2010.04
    -
    Present

    会計幹事, 日本材料学会 生体・医療材料部門

  • 2009.04
    -
    2010.03

    庶務幹事, 日本材料学会 生体・医療材料部門