Onoe, Hiroaki

写真a

Affiliation

Faculty of Science and Technology, Department of Mechanical Engineering （Yagami）

Position

Professor

Related Websites
Page Top ▲

Career 【 Display / hide 】

  • 2005.04
    -
    2007.03

    The University of Tokyo, Graduate School of Information Science and Technology, JSPS Research Fellowship for Young Scientists (DC2-PD)

  • 2007.04
    -
    2009.03

    The University of Tokyo, Institute of Industrial Science, JSPS Research Fellowship for Young Scientists (PD)

  • 2007.08
    -
    2009.01

    University of California Berkeley, Department of Chemistry, Visiting Scholar

  • 2009.04
    -
    2014.03

    The University of Tokyo, Institute of Industrial Science, Assistant Professor

  • 2010.10
    -
    2014.05

    Japan Science and Technology Agency, ERATO assistant research director

display all >>

Page Top ▲

Academic Background 【 Display / hide 】

  • 1997.04
    -
    2001.03

    The University of Tokyo, School of Engineering, Department of Mechano-Informatics

    University, Graduated

  • 2001.04
    -
    2006.03

    The University of Tokyo, Graduate School of Information Science and Technology, Department of Mechano-Informatics

    Graduate School, Completed, Doctoral course

Page Top ▲

Academic Degrees 【 Display / hide 】

  • 博士（情報理工学）, The University of Tokyo, Coursework, 2006.03

    マイクロ構造体の順序付き自己組立て

Page Top ▲
 

Research Areas 【 Display / hide 】

  • Nanotechnology/Materials / Nano/micro-systems

  • Informatics / Mechanics and mechatronics

  • Informatics / Robotics and intelligent system

  • Life Science / Biomedical engineering

  • Life Science / Biomaterials

Page Top ▲

Research Keywords 【 Display / hide 】

  • Micro-Nano system

  • Microfluidics

  • Self-assembly

  • Soft material

  • Tissue engineering

Page Top ▲
 

Books 【 Display / hide 】

  • マイクロ・ナノ熱工学の進展

    吉田光輝, 尾上弘晃, NTS, 2021.05

    Scope: 熱駆動マイクロゲルデバイス,  Contact page： 505-513

  • Integrated microsystems for bridging multiscale elements

    Yoshida K., Onoe H., Advances in Chemical Engineering, 2021.01

    　View Summary

    Hydrogels are emerging as enabling materials for a wide range of new applications for soft robots because of their flexibility. Some of the polymer chains that make up the hydrogel have a nano-scale function of swelling and contracting in response to external stimuli. In addition, because the network of polymer chains is on the nanometer order, it is possible to encapsulate various functional materials. Therefore, stimulus-responsive hydrogels and functional materials encapsulating hydrogels are also being actively studied for use as soft robot components. From a fundamentals point of view, the nano-scale functions of hydrogels, fabrication method, and integration with functional materials must be considered for each specific application. This section provides a basic understanding of hydrogels and the recent development of novel fabrication and integration of hydrogel with functional materials such as magnetic nanoparticles, Pt catalyst, graphene, photonic colloidal crystal, and living cells.

  • Microspring Fabrication by Anisotropic Gelation (Micro and Nano Fabrication Technology)

    Hiroaki Onoe, Koki Yoshida, Springer Nature Singapore, 2018.04

    Scope: pp. 1-20

  • ハイドロゲルをマイクロスケールで精密加工するには？（ゲル化・増粘剤の使い方，選び方　事例集）

    中島駿介，尾上 弘晃, 技術情報協会, 2018.02

    Scope: pp. 374-382

  • Fabrication of 3D cellular tissue utilizing MEMS technologies (Hyper Bio Assembler for 3D Cellular Systems)

    Shotaro Yoshida, Daniela Serien, Fumiaki Tomoike, Hiroaki Onoe, Shoji Takeuchi, Springer, 2015.07

    Scope: pp. 177-202

display all >>

Page Top ▲

Papers 【 Display / hide 】

  • Adeno-Associated Virus-Encapsulated Alginate Microspheres Loaded in Collagen Gel Carriers for Localized Gene Transfer

    Kurashina Y., Kurihara S., Kubota T., Takatsuka S., Hirabayashi M., Shimmura H., Miyahara H., Hioki A., Matsushita Y., Muramatsu J., Ogawa Y., Fujioka M., Okano H.J., Onoe H.

    Advanced Healthcare Materials （Advanced Healthcare Materials)  13 （ 12 ）  2024.05

    ISSN  21922640

    　View Summary

    This work reports localized in vivo gene transfer by biodegradation of the adeno-associated virus-encapsulating alginate microspheres (AAV-AMs) loaded in collagen gel carriers. AAV-AMs are centrifugally synthesized by ejecting a mixed pre-gel solution of alginate and AAV to CaCl2 solution to form an ionically cross-linked hydrogel microsphere immediately. The AAV-AMs are able to preserve the AAV without diffusing out even after spreading them on the cells, and the AAV is released and transfected by the degradation of the alginate microsphere. In addition, AAV-AMs can be stored by cryopreservation until use. By implanting this highly convenient AAV-encapsulated hydrogel, AAV-AMs can be loaded into collagen gel carriers to fix the position of the implanted AAV-AMs and achieve localized gene transfer in vivo. In vivo experiments show that the AAV-AMs loaded in collagen gel carriers are demonstrated to release the encapsulated AAV for gene transfer in the buttocks muscles of mice. While conventional injections caused gene transfer to the entire surrounding tissue, the biodegradation of AAV-AMs shows that gene transfer is achieved locally to the muscles. This means that the proposed AAV-loaded system is shown to be a superior method for selective gene transfer.

  • Light-Triggered Transmittance Control in Thermoresponsive Hydrogels by Femtosecond Laser Direct Writing

    Kashikawa K., Tomikawa H., Onoe H., Terakawa M.

    ACS Applied Optical Materials （ACS Applied Optical Materials)  2 （ 4 ） 565 - 573 2024.04

    　View Summary

    Thermoresponsive hydrogels, which exhibit changes in their optical properties and volume due to temperature variations, are promising candidates for applications in soft devices. In this study, we demonstrate the modulation of transmittance in a thermoresponsive hydrogel through light stimulation employing gold microstructures fabricated via multiphoton photoreduction. The spatial integration of photoresponsiveness, attributed to high-density gold nanoparticles within the thermoresponsive hydrogel, was accomplished through the high-speed laser scanning of femtosecond laser pulses. The temperature measurement during the fabrication of the gold microstructure revealed that the high-speed and multiple scanning over the same path effectively reduced the temperature in the irradiated area of femtosecond laser pulses. The present approach enabled the mitigation of thermal effects during the fabrication, resulting in minimizing distortion in the fine lines of the structures. Upon exposure to stimulus light, a rapid change in the transmittance of the region where the structures were fabricated was prominently observed. The present method unveils a promising avenue for the advancement of light-responsive soft devices.

  • In-line micro-spectrometer with a structural color polymer filter for the color and concentration monitoring system

    Nishita S., Onoe H.

    Sensors and Actuators A: Physical （Sensors and Actuators A: Physical)  367 2024.03

    ISSN  09244247

    　View Summary

    Monitoring systems for color and concentration of liquids have an important role in improving the fabrication process stably, not only in pipelines but also in microchannels. Our suggested in-line micro-spectrometer with an elastic structural color filter has detectability for color and concentration changes with a 40-mm size. The sequence of our device is the following: (1) the elastic structural color filter splits the white light from an LED, (2) the split light transmits the sample liquid, and (3) a photodiode receives the transmitted light. The structural color polymer is mechanically compressed inside the micro-spectrometer, leading to fixing the optical path even though the light wavelength is changed continuously. We fabricated the elastic structural color polymer with 200 nm silica nanoparticles and PEGPEA (poly- (ethylene glycol) phenyl ether acrylate). The prototype of the in-line micro-spectrometer with a red (λ = 596 nm) filter showed the detectability of the difference between yellow and green dyes. Moreover, the micro-spectrometer successfully distinguished the concentration of green-dyed water. This proposed in-line micro-spectrometer would contribute to developing in-line microfluidic color monitoring systems for biochemical analyses and material production.

  • Mucin-Layer-Secreting in Vitro Intestinal Tube-Shaped Device with Cryptic Structure for Bacterial Co-Culture

    Uramoto S., Tanaka S., Itai S., Onoe H.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) （Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS))     485 - 488 2024

    ISSN  10846999

    　View Summary

    We propose an in vitro intestinal tube-shaped device with a secreted mucin layer that enables us to achieve stable and continuous co-culturing with bacteria. Our tube-shaped device is made of collagen hydrogel and has 3D crypt-like structures created by electrolytically generated microbubbles. Bacterial suspension can be perfused via glass/silicone tubes attached at both ends of the tube device. Supply of culture medium diffused from the outside of the tube ensures a stable culture of Caco-2 and HT29 cells on the inside of the tube. We confirmed that HT29 cells mainly secreted a mucin layer in the apical portion of cryptic structures that spatially separates the intestinal cells and bacteria as seen in vivo environments. As a result, the co-culture of intestinal cells and lactobacillus in vitro was demonstrated in the 3D crypt-like structures in our intestinal tube-shaped device.

  • Collagen Hollow Microbeads for Engineered Hollow Organ Model in Vitro

    Abeta S., Masuda A., Hioki A., Shoji K., Onoe H.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) （Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS))     477 - 480 2024

    ISSN  10846999

    　View Summary

    We propose an in vitro model to mimic the hollow structure of biological tissues (e.g. heart or stomach) using hollow core-shell collagen microgel beads. By using collagen hydrogel as the shell of the microbeads, cells encapsulated inside of the collagen shell can proliferate and form a tissue structure with a hollow center. In this paper, as a first step toward engineered hollow structure tissue formation, we report a fabrication method for core-shell (hollow) alginate microgel beads by a simple droplet-dipping method using a two-layer coaxial nozzle device. To apply collagen hydrogel to this droplet-dipping method, we applied alginate cross-linking network as a template for creating collagen microgel beads and dissolved the alginate network after the collagen hydrogel was stabilized. By combining these techniques, we believe that hollow collagen microgel beads encapsulating cells can easily and massively be produced for reconstructing in vitro hollow organ models.

display all >>

Page Top ▲

Papers, etc., Registered in KOARA 【 Display / hide 】

display all >>

Page Top ▲

Reviews, Commentaries, etc. 【 Display / hide 】

  • Can nanoparticles enhance drug-delivery performance of hydrogels?

    Rossi F., Kurashina Y., Onoe H.

    Nanomedicine (Nanomedicine)  18 ( 8 ) 653 - 657 2023.04

    ISSN  17435889

  • 3次元灌流共培養のためのマイクロゲルチューブデバイス

    板井駿，尾上弘晃

    ケミカルエンジニヤリング 63 ( 11 ) 52 - 57 2018.11

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

  • 細胞ファイバの形成技術と再生組織移植への応用

    尾上弘晃, 興津輝

    実験医学 33 ( 8 ) 1235 - 1241 2015

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

  • 生体組織構築のための細胞ファイバ技術

    尾上弘晃, 竹内昌治

    生物物理 55 ( 4 ) 206 - 207 2015

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

  • 曲面や粗面をつかむロボットハンドの実現に求められる樹脂吸盤 –そのニーズと開発事例-

    西田知司, 尾上弘晃

    MATERIAL STAGE 15 ( 3 ) 63 - 67 2015

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Joint Work

display all >>

Page Top ▲

Presentations 【 Display / hide 】

  • Microfluidic reflective display with primary color sub-pixels

    Junpei Muramatsu, Hiroaki Onoe

    The 33rd International Conference on Micro Electro Mechanical Systems (MEMS 2020), 

    2020.01
    , 

    Poster presentation

  • Locally bendable stimuli-responsive hydrogel actuator with axially patterned functional materials

    Nobuki Takeuchi, Shunsuke Nakajima, Yutaka Hori, Ryuji Kawano, Hiroaki Onoe

    The 33rd International Conference on Micro Electro Mechanical Systems (MEMS 2020), 

    2020.01
    , 

    Oral presentation (general)

  • pNIPAM/SWCNT-based hydrogel micro-gripper driven by infrared light for intravascular surgery

    Takaya Kuroda, Hiroaki Onoe

    The 33rd International Conference on Micro Electro Mechanical Systems (MEMS 2020), 

    2020.01
    , 

    Poster presentation

  • Self-folding acute-angel origami driven by surface bending force

    Takuya Uchida, Hiroki Yasuga, Tomohiro Tachi, Eiji Iwase, Hiroaki Onoe

    The 33rd International Conference on Micro Electro Mechanical Systems (MEMS 2020), 

    2020.01
    , 

    Poster presentation

  • ECM-based gradient generator for tunable surface environment by interstitial flow

    Azusa Shimizu, Wei H. Goh, Rahul Karyappa, Michinao Hashimoto, Hiroaki Onoe

    The 33rd International Conference on Micro Electro Mechanical Systems (MEMS 2020), 

    2020.01
    , 

    Oral presentation (general)

display all >>

Page Top ▲

Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • 三次元組織の高度成熟化を自律的に達成する知能化培養システム基盤の創出

    2023.04
    -
    2026.03

    基盤研究(A), Principal investigator

  • 機械的メタマテリアルとDNAゲルの融合による生化学構造色センサの高感度化

    2021.07
    -
    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Challenging Research (Pioneering), Principal investigator

  • 力学刺激の知能化によるin vitro3次元組織の超効率的成熟化

    2019.04
    -
    2022.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (A) , Principal investigator

  • ナノグルコースセンサが取り込まれた人工組織の開発

    2016.09
    -
    2018.03

    日本学術振興会, Grant-in-Aid for Scientific Research, Research grant, Principal investigator

  • 炎症が惹起する神経変性機構解明のためのヒト血液脳関門の構築

    2016.04
    -
    2018.03

    日本学術振興会, Grant-in-Aid for Scientific Research, Research grant, Coinvestigator(s)

display all >>

Page Top ▲

Works 【 Display / hide 】

  • Biohybird Art

    Biohybrid Art Lab with Fu Tsurumaki

    Spiral Independent Creator’s Festival, 2011, 

    2010.05
    , 

    Other, Joint

Page Top ▲

Intellectual Property Rights, etc. 【 Display / hide 】

  • 給電型遠心駆動微小液滴生成装置およびその生成物

    Date applied： 特願2018-76379  2018.05 

    Patent, Joint

  • 多層構造体とその製造方法及び利用方法

    Date applied： 特願2017-92602  2017.05 

    Patent, Joint

  • 刺激応答性ファイバ，刺激応答性ファイバの製造方法，及び刺激応答性ファイバの製造装置

    Date applied： 特願2016-199517  2016.10 

    Patent, Single

  • カラーフィルタ、これを用いた表示装置、及びカラーフィルタの作製方法

    Date applied： 特願2016-10932  2016.01 

    Patent, Joint

  • マイクロビーズ及びその製造方法

    Date applied： 特願2015-210971  2015.10 

    Patent, Joint

display all >>

Page Top ▲

Awards 【 Display / hide 】

  • The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology

    2017.04, Minister of Education, Culture, Sports, Science and Technology, マイクロ加工技術を利用した人工生体組織構築の研究

  • 第４回新化学技術研究奨励賞

    尾上弘晃, 2014.05, 公益財団法人 新化学技術推進協会, マイクロゲルファイバのself-foldingによる複合3次元機能材料構築法の創生

  • Outstanding paper award

    1. Yuya Morimoto, Hiroaki Onoe, Shoji Takeuchi, 2013, Twenty Sixth International Conference on Micro Electro Mechanical Systems, Muscle Based Bioactuator Driven in Air

    Type of Award： International academic award (Japan or overseas)

  • Igarashi Prize (Best presentation award)

    2011.09, IEEJ sensors and micromachines, Construction of centimeter-scale three-dimensional tissue with cell fibers

  • JIEP best paper award

    3. Tetsuo Kan, Yusuke Takei, Hiroaki Onoe, Eiji Iwase, Tetsuji Dohi, Kiyoshi Matsumoto, Isao Shimoyama, 2009, The International Conference on Electronics Packaging, Nano-Mechanical Structure Fabrication Technology for Highly Integrated, Complex MEMS

    Type of Award： International academic award (Japan or overseas)

display all >>

Page Top ▲
 

Courses Taught 【 Display / hide 】

  • SPECIAL LECTURE SERIES ON MULTIDISCIPLINARY AND DESIGN SCIENCE

    2024

  • LABORATORIES IN SCIENCE AND TECHNOLOGY

    2024

  • INTRODUCTION TO MECHANICS OF MATERIALS

    2024

  • INDEPENDENT STUDY ON INTEGRATED DESIGN ENGINEERING

    2024

  • INDEPENDENT STUDY FOR EXCHANGE STUDENT B

    2024

display all >>

Page Top ▲

Courses Previously Taught 【 Display / hide 】

  • 機械工学実験

    Keio University

    2015.04
    -
    2016.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Outside own faculty (within Keio), 3h, 140people

  • 機械工学創造演習

    Keio University

    2015.04
    -
    2016.03
    , 

    Autumn Semester, Seminar, Outside own faculty (within Keio), 2h, 140people

  • 理工学基礎実験

    Keio University

    2015.04
    -
    2016.03
    , 

    Spring Semester, Laboratory work/practical work/exercise, Outside own faculty (within Keio), 2h

  • プロダクションエンジニアリング

    Keio University

    2015.04
    -
    2016.03
    , 

    Spring Semester, Seminar, Outside own faculty (within Keio), 2h, 140people

  • 機械工学創造演習

    Keio University

    2014.04
    -
    2015.03
    , 

    Autumn Semester, Seminar

display all >>

Page Top ▲
 

Memberships in Academic Societies 【 Display / hide 】

  • IEEE, 

    2016
    -
    Present

  • 電気学会, 

    2016
    -
    Present

  • Japanese Society of Mechanical Engineering, 

    2013
    -
    Present

  • Society for Chemistry and Micro-Nano Systems, 

    2010
    -
    Present

  • The Biophysical Society of Japan, 

    2009
    -
    Present
Page Top ▲

Committee Experiences 【 Display / hide 】

  • 2017.04
    -
    Present

    広報担当・幹事, 日本機械学会マイクロ・ナノ工学部門　総務委員会

  • 2017.02
    -
    2018.01

    主査, 電気学会　第34回センサ・マイクロマシンと応用システムシンポジウム論文委員会

  • 2017.02
    -
    2018.01

    広報委員, 日本機械学会　第8回マイクロ・ナノ工学　実行委員会

  • 2017.01
    -
    2019.12

    委員, 電気学会　立体構造や柔軟材料への微細加工、実装技術に関する若手研究者を中心とした調査専門委員会

  • 2016.02
    -
    2017.01

    副主査, 電気学会　第33回センサ・マイクロマシンと応用システムシンポジウム論文委員会

display all >>

Page Top ▲