MATSUZAKI Juntaro

写真a

Affiliation

Faculty of Pharmacy, Department of Pharmacy Division of Pharmacotherapeutics (Shiba-Kyoritsu)

Position

Associate Professor

E-mail Address

E-mail address

Related Websites

External Links

 

Research Areas 【 Display / hide

  • Tumor diagnostics

  • Gastroenterology

Research Keywords 【 Display / hide

  • cancer

  • microRNA

  • extracellular RNA

 

Books 【 Display / hide

  • 血中マイクロRNAを用いた膵がん診断の展望. 早期発見・予防に向けた次世代がん検査技術の最前線

    松﨑潤太郎, シーエムシー出版, 2019

  • 胃・十二指腸潰瘍. 看護基礎教育テキスト ナーシング・グラフィカ「疾患と看護シリーズ」『3 消化器疾患と看護』

    松﨑潤太郎, 鈴木秀和, メディカ出版, 2019

  • がん早期診断. テクノロジーロードマップ2019-2028 医療・健康・食農編

    松﨑潤太郎, 落谷孝広, 日経BP, 2019

  • Bile acids and esophageal cancer

    J Matsuzaki, H Suzuki, 2017

  • エクソソームに着目した新しい診断技術の開発. 早期発見・低侵襲で見つけ出す疾患・病態検査・診断法の開発 ~がん、認知症、感染症、難病、糖尿病、リウマチ、希少疾患~

    松﨑潤太郎, 落谷孝広, 技術情報協会, 2017

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Papers 【 Display / hide

  • A novel combination of serum microRNAs for the detection of early gastric cancer

    S Abe, J Matsuzaki, K Sudo, I Oda, H Katai, K Kato, S Takizawa, ...

    Gastric Cancer, 1-9 (Gastric Cancer)   2021.03

    ISSN  1436-3291

     View Summary

    Background: The aim of this study was to identify serum miRNAs that discriminate early gastric cancer (EGC) samples from non-cancer controls using a large cohort. Methods: This retrospective case–control study included 1417 serum samples from patients with EGC (seen at the National Cancer Center Hospital in Tokyo between 2008 and 2012) and 1417 age- and gender-matched non-cancer controls. The samples were randomly assigned to discovery and validation sets and the miRNA expression profiles of whole serum samples were comprehensively evaluated using a highly sensitive DNA chip (3D-Gene ) designed to detect 2565 miRNA sequences. Diagnostic models were constructed using the levels of several miRNAs in the discovery set, and the diagnostic performance of the model was evaluated in the validation set. Results: The discovery set consisted of 708 samples from EGC patients and 709 samples from non-cancer controls, and the validation set consisted of 709 samples from EGC patients and 708 samples from non-cancer controls. The diagnostic EGC index was constructed using four miRNAs (miR-4257, miR-6785-5p, miR-187-5p, and miR-5739). In the discovery set, a receiver operating characteristic curve analysis of the EGC index revealed that the area under the curve (AUC) was 0.996 with a sensitivity of 0.983 and a specificity of 0.977. In the validation set, the AUC for the EGC index was 0.998 with a sensitivity of 0.996 and a specificity of 0.953. Conclusions: A novel combination of four serum miRNAs could be a useful non-invasive diagnostic biomarker to detect EGC with high accuracy. A multicenter prospective study is ongoing to confirm the present observations. ®

  • Precision medicine approaches to prevent gastric cancer

    J Matsuzaki, H Tsugawa, H Suzuki

    Gut and Liver 15 (1), 3 (Gut and Liver)  15 ( 1 ) 3 - 12 2021.01

    ISSN  1976-2283

     View Summary

    © Gut and Liver. Gastric cancer remains one of the most common causes of cancer-related death worldwide, although the incidence is declining gradually. The primary risk factor for gastric cancer is Helicobacter pylori infection. The Kyoto global consensus report recommends eradication of H. pylori in all infected patients. However, because it is difficult to stratify the risk of carcinogenesis among patients with a history of H. pylori infection, annual endoscopic surveillance is performed for everyone after eradication. This review summarizes the current approaches used to screen for novel molecules that could assist in the diagnosis of gastric cancer and reduce mortality. Most well-studied molecules are tissue protein biomarkers expressed by the gastric epithelium and associated with metaplasia-dysplasia-carcinoma sequences. Other strategies focus on the origin of cancer stem cell-related markers, such as CD44, and immune reaction-related markers, such as matrix metallopeptidases. Noninvasive methods such as blood-based approaches are more attractive. Serum pepsinogen levels predict the severity of gastric mucosal atrophy before H. pylori eradication, whereas plasma ghrelin levels are associated with atrophy even after eradication. Cell-free DNAs and RNAs are attractive tools for the early detection of cancer. These ideas could lead to the development of more personalized strategies for cancer prevention based on cutting-edge technologies.

  • Development of diagnostic models for solid cancers using circulating microRNAs

    J Matsuzaki

    CANCER SCIENCE 112, 203-203  2021

  • Circulating microRNAs: Next-generation Cancer Detection

    J Matsuzaki, T Ochiya

    The Keio journal of medicine 69 (4), 88-96 (Keio Journal of Medicine)  69 ( 4 ) 88 - 96 2020.12

    ISSN  0022-9717

     View Summary

    © 2020 by The Keio Journal of Medicine. Early detection of cancer is crucial for its ultimate control and the prevention of malignant progression. In Japan, a nationwide project was conducted between 2014 and 2019 to develop novel cancer detection tools using serum microRNAs (miRNAs). Using the National Cancer Center Biobank, we collected more than 10,000 serum samples from patients with malignant diseases, including rare cancers such as ovarian cancer, gliomas, and sarcomas. Subsequently, comprehensive miRNA microarray analyses were performed for all samples. This serum miRNA database provides insights regarding miRNA bio-marker candidates for each cancer type. Here, we summarize the major achievements of this national project. Notably, although circulating miRNAs packaged in extracellular vesicles are thought to be a cell-to-cell communication tool, the functional characteristics of the miRNAs listed in the project are still unknown. We hope that our findings will help elucidate the biological functions of circulating miR-NAs.

  • Extracellular miRNAs for the Management of Barrett’s Esophagus and Esophageal Adenocarcinoma: A Systematic Review

    K Inokuchi, T Ochiya, J Matsuzaki

    Journal of Clinical Medicine 10 (1), 117 10 ( 1 )  2020.12

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Reviews, Commentaries, etc. 【 Display / hide

  • 【大腸癌診療におけるprecision medicine】大腸癌患者の血清miRNAを用いた術前リンパ節転移予測診断能の意義について

    森谷 弘乃介, 松崎 潤太郎, 高見澤 康之, 坂本 良平, 塚本 俊輔, 志田 大, 金光 幸秀

    癌の臨床 ((株)篠原出版新社)  65 ( 4 ) 345 - 351 2021.01

    ISSN  0021-4949

  • 血液マイクロRNAによる子宮肉腫と変性筋腫の術前鑑別診断

    加藤 友康, 横井 暁, 松崎 潤太郎, 山本 雄介, 舘 慶生, 米岡 完, 清水 華子, 植原 貴史, 石川 光也, 滝澤 聡子, 青木 良晃, 加藤 健, 落谷 孝広, 東レ株式会社新事業開発部門DNAチップG

    日本婦人科腫瘍学会雑誌 ((公社)日本婦人科腫瘍学会)  39 ( 1 ) 297 - 297 2021.01

    ISSN  1347-8559

  • 慢性便秘診療の新展開 上皮機能変容薬の反応性に関する慢性便秘患者の患者背景の検討

    猪口 和美, 正岡 建洋, 松崎 潤太郎, 金井 隆典

    日本消化管学会雑誌 ((一社)日本消化管学会)  5 ( Suppl. ) 254 - 254 2021.01

    ISSN  2433-3840

  • ペニシリンアレルギー患者に対するボノプラザン、シタフロキサシン、メトロニダゾール含有レジメンの評価

    猪口 和美, 正岡 建洋, 森 英毅, 鈴木 秀和, 松崎 潤太郎, 岡沢 啓, 吉岡 政洋, 金井 隆典

    日本ヘリコバクター学会学術集会プログラム・抄録集 ((一社)日本ヘリコバクター学会)  26回   95 - 95 2020.12

  • 血中microRNAによる固形がん診断モデルの構築

    松崎 潤太郎

    日本癌学会総会記事 (日本癌学会)  79回   YIA - 1 2020.10

    ISSN  0546-0476

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Presentations 【 Display / hide

  • Prediction of cancer tissue of origin by circulating miRNA profiles

    The 12th Scientific Workshop of the Early Detection Research Network, 2021.03

  • Prospects of cancer diagnosis by circulation miRNA profiles

    International Session 5 “Innovative technology for next generation liquid biopsy” 第78回日本癌学会学術総会, 2019.09

  • 低分子化合物を用いた成熟肝・膵実質細胞の前駆細胞へのリプログラミング技術の開発

    一般口演19 「細胞-1/口腔/酸化ストレス」. 第19回日本抗加齢医学会総会, 2019.06

  • 低分子化合物による膵外分泌細胞の膵前駆様細胞およびインスリン分泌細胞の誘導

    口演33 「膵臓2」. 第18回日本再生医療学会総会, 2019.03

  • Circulating microRNA profiling for the detection of cancers: recent progress

    The 34th Joint Annual Conference of Biomedical Science, 2019.03

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • CRISPR-Cas9 based screening for essential tumor suppressors in liver carcinogenesis

    2017.06
    -
    2020.03

    National Cancer Center Japan, Yamamoto Yusuke, 松崎 潤太郎, Grant-in-Aid for Challenging Research (Exploratory)

     View Summary

    A large number of research projects identified genetic mutations in the process of carcinogenesis, and there have been some useful findings of studies using cancer cell lines. With next generation sequencing, genetic variants in a variety of cancers have been identified and shown to have an impact on carcinogenesis; however, it is still unclear how the mutations essentially influenced the carcinogenesis and their metastatic potential. In this research project, we introduced and quantitatively analyzed the contribution of genetic mutations involved in the carcinogenic process by CRISPR-Cas9, in order to knockout the tumor suppressor genes in primary epithelial cells, and examined the effect of each gene on carcinogenesis. Also, we tried to recapitulate the multi-step carcinogenesis in vitro. This study enables us to accurately reflect and reproduce genomic aberrations in normal cells, and to achieve validation of the actual function of driver gene mutations.

  • Identification of circulating microRNAs for the detection of early stage pancreatic cancer

    2017.04
    -
    2020.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, 松崎 潤太郎, Grant-in-Aid for Scientific Research (C), Principal Investigator

     View Summary

    本研究は、初代分離培養した膵外分泌細胞に対して低分子化合物を曝露させ、in vitroでパーシャルリプログラミングを行って膵前駆細胞を樹立し、この細胞に胆汁酸によるストレス刺激を与えることで膵発がん初期の環境をin vitroでモデル化し、膵発がん超初期のバイオマーカーとなるmicroRNAの同定を試みるものである。我々はラットおよびマウスの膵外分泌細胞を用いて、特定の低分子化合物のコンビネーションが膵前駆細胞を誘導することを見出したが、一方で誘導された膵前駆細胞の性能を、Pdx1やNkx6.1といったマーカー分子で評価したところ、実験ロットによって均質でないことを見出した。そこでsingle cell sortingによって、膵前駆細胞としてより機能的に優れた細胞のクローン化を試み、これに成功した。驚くべきことに、この膵前駆細胞は特定の培養環境下においてインスリンを分泌する細胞にも分化し得た。また膵外分泌細胞よりDBAレクチンを用いて分取した膵管上皮細胞が膵前駆細胞の由来細胞であることも見出した。
    機能的に優れた膵前駆細胞のクローニングに成功した。この細胞に胆汁酸を曝露した際に濃度依存的に変動する細胞内/細胞外マイクロRNAのマイクロアレイ解析も完了し、データ解析を進めている。
    胆汁酸曝露により変動するマイクロRNA候補を同定し、すでに完成しているヒト血清マイクロRNAデータベースと照合することにより、膵がん早期診断マーカーとしてのポテンシャルに優れたマイクロRNAを絞り込む。胆汁酸曝露によるマイクロRNA発現調節機序を解析する。

  • Generation of hepatic progenitor cells from human hepatocytes using small molecules

    2016.04
    -
    2018.03

    National Cancer Center Japan, KATSUDA Takeshi, OCHIYA Takahiro, MATSUZAKI Juntaro, SAITO Yoshimasa, TAKEUCHI Atsuko, Grant-in-Aid for Young Scientists (B)

     View Summary

    Using small molecule inhibitors, we recently reported that rodent mature hepatocytes can be reprogrammed into progenitor-like phenotype with repopulative capacity. In this study, using the same strategy, we demonstrated that hepatic progenitor cells can be induced from human infant hepatocytes. These cells, named human chemically induced progenitors (hCLiPs), exhibited significant repopulative capacity in injured mouse livers following transplantation, and contributed to reconstruction of the normal liver architecture. We also found that hCLiPs can be redifferentiated into mature hepatocytes in vitro with hepatic inducible factors. These redifferentiated cells can be induced to exhibit cytochrome P450 (CYP) enzymatic activities in response to the CYP inducing molecules with the efficiency comparable with that of primary hepatocytes. Thus, this study contributes to progress in the field of liver cell transplantation therapy and pharmacological research field.

  • Interaction of miR-221/222 and adipocytokine signaling in esophageal adenocarcinoma

    2014.04
    -
    2016.03

    Keio University, Matsuzaki Juntaro, TSUGAWA Hitoshi, SUZUKI Hidekazu, Grant-in-Aid for Young Scientists (B)

     View Summary

    Esophageal adenocarcinoma, which is rapidly increasing in Western countries, is one of the complications of gastroesophageal reflux disease. Bile reflux and central obesity are major risk factors for esophageal adenocarcinoma. In general, plasma adiponectin levels are decreased through the progression of central obesity. We had reported that bile acids contribute to carcinogenesis through the activation of nuclear bile acid receptor (FXR) and the enhancement of miR-221/222. Herein, we identified that miR-221/222 enhance the expression of COX-2 in esophageal epithelial cells. On the other hand, COX-2 expression was suppressed during exposure to adiponectin receptor agonist. These results suggest adiponectin receptor agonist might work as a chemopreventive agent for esophageal adenocarcinoma among high-risk individuals with central obesity.

  • 食道腺がん治療標的としてのCDX2分解系とmiR-221/222の重要性の解明

    2011.04
    -
    2012.03

    Keio University, 松崎 潤太郎, Grant-in-Aid for Young Scientists (B)

Awards 【 Display / hide

  • 日本癌学会奨励賞

    2020

    Type of Award: Awards of National Conference, Council and Symposium

  • the 15th Korea-Japan Joint Symposium on Helicobacter infection Young Investigator Award

    2018

    Type of Award: Awards of International Conference, Council and Symposium

  • United European Gastroenterology Week (UEGW) 2016 Poster of Excellence

    2016

    Type of Award: Awards of International Conference, Council and Symposium

  • 第18回日本神経消化器病学会・第6回IBS研究会・第84回消化器心身医学研究会・第10回機能性ディスペプシア研究会合同学術集会 並木賞

    2016

    Type of Award: Awards of National Conference, Council and Symposium

  • 4th Biennial Congress of Asian Neurogastroenterology & Motility Association (ANMA) Best Poster Presentation Award

    2015

    Type of Award: Awards of International Conference, Council and Symposium

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Courses Taught 【 Display / hide

  • STUDY OF MAJOR FIELD: (PHARMACOTHERAPEUTICS)

    2021

  • SEMINAR:(PHARMACOTHERAPEUTICS)

    2021

  • RESEARCH FOR BACHELOR'S THESIS 1

    2021

  • PRE-CLINICAL TRAINING FOR HOSPITAL & COMMUNITY PHARMACY

    2021

  • PHYSICAL ASSESSMENT AND DIAGNOSTIC IMAGING

    2021

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Committee Experiences 【 Display / hide

  • 2019
    -
    Present

    日本微小循環学会評議員

  • 2018
    -
    Present

    日本ヘリコバクター学会代議員