Wakui, Masatoshi

写真a

Affiliation

School of Medicine, Department of Laboratory Medicine (Shinanomachi)

Position

Associate Professor

External Links

Other Affiliation 【 Display / hide

  • (兼務所属)慶應義塾大学病院臨床研究推進センター, 再生医療等支援部門, センター員

Career 【 Display / hide

  • 1989
    -
    1991

    慶應義塾大学医学部研修医(内科)

  • 1991
    -
    1993

    慶應義塾大学医学部助手(専修医)(内科学)

  • 1991
    -
    1993

    横浜市立市民病院内科

  • 1993
    -
    1995

    慶應義塾大学医学部内科学教室血液研究室(専修医)

  • 1995
    -
    1997

    慶應義塾大学助手(医学部内科学)

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Academic Background 【 Display / hide

  • 1983.04
    -
    1989.03

    Keio University, School of Medicine, 医学科

    University, Graduated

Academic Degrees 【 Display / hide

  • 博士(医学), Keio University, Dissertation, 2006.09

    Genetic dissection of systemic lupus erythematosus pathogenesis : partial functional complementation between Sle1 and Sle3/5 demonstrates requirement for intracellular coexpression for full phenotypic expression of lupus(全身性エリテマトーデスの成因に関する遺伝学的解析 : Sle1とSle3/5の部分的機能補完は、完全なループス表現型発現のために細胞内共発現が要求されることを示す)

Licenses and Qualifications 【 Display / hide

  • 日本専門医機構基本領域臨床検査専門医, 日本専門医機構による認定, 2023.01

  • 臨床研修指導医, 第16回慶應義塾大学病院臨床研修指導医養成WS受講

  • 医師免許, 1989.05

  • 臨床検査専門医, 日本臨床検査医学会認定, 2017.09

  • 臨床検査管理医, 2017.12

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Research Areas 【 Display / hide

  • Life Science / Genome biology

  • Life Science / Pathological biochemistry

  • Life Science / Immunology

  • Life Science / Tumor biology

  • Life Science / Tumor diagnostics and therapeutics

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Books 【 Display / hide

  • 遺伝子検査技術〜遺伝子分析科学認定士テキスト〜改訂第3版

    公益社団法人 日本臨床検査同学院編, 宇宙堂八木書店, 2023.03

    Scope: 血液学

  • 標準臨床検査医学(第5版)

    高木康、山田俊幸編, 医学書院, 2023.02

  • スタンダード検査血液学(第4版)

    日本検査血液学会編, 医歯薬出版株式会社, 2021.04

  • 検査値の読み方・考え方〜専門医からのアドバイス(第3版)

    WAKUI Masatoshi, 総合医学社, 2021

  • 臨床検査ガイド2020〜2021

    2019

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Papers 【 Display / hide

  • ホットトピックス:凝固(出血)分野

    涌井昌俊、長江千愛、武山雅博

    血栓止血誌 35 ( 1 ) 印刷中 2024

    Research paper (scientific journal), Lead author, Corresponding author

  • Association of systemic adverse reaction patterns with long-term dynamics of humoral and cellular immunity after coronavirus disease 2019 third vaccination

    Yoshida M, Kobashi Y, Kawamura T, Shimazu Y, Nishikawa Y, Omata F, Saito H, Yamamoto C, Zhao T, Takita M, Ito N, Tatsuno K, Kaneko Y, Nakayama A, Kodama T, Wakui M, Takahashi K, Tsubokura M

    Sci Rep (Scientific Reports)  13 ( 1 ) 9264 2023.06

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    The objective of this study was to clarify the impact of adverse reactions on immune dynamics. We investigated the pattern of systemic adverse reactions after the second and third coronavirus disease 2019 (COVID-19) vaccinations and their relationship with immunoglobulin G against severe acute respiratory syndrome coronavirus 2 spike 1 protein titers, neutralizing antibody levels, peak cellular responses, and the rate of decrease after the third vaccination in a large-scale community-based cohort in Japan. Participants who received a third vaccination with BNT162b2 (Pfizer/BioNTech) or mRNA-1273 (Moderna), had two blood samples, had not had COVID-19, and had information on adverse reactions after the second and third vaccinations (n = 2198) were enrolled. We collected data on sex, age, adverse reactions, comorbidities, and daily medicine using a questionnaire survey. Participants with many systemic adverse reactions after the second and third vaccinations had significantly higher humoral and cellular immunity in the peak phase. Participants with multiple systemic adverse reactions after the third vaccination had small changes in the geometric values of humoral immunity and had the largest geometric mean of cellar immunity in the decay phase. Systemic adverse reactions after the third vaccination helped achieve high peak values and maintain humoral and cellular immunity. This information may help promote uptake of a third vaccination, even among those who hesitate due to adverse reactions.

  • Varying cellular immune response against SARS-CoV-2 after the booster vaccination: A cohort study from Fukushima Vaccination Community Survey, Japan

    Tani Y, Takita M, Kobashi Y, Wakui M, Tianchen Z, Yamamoto C, Saito H, Kawashima M, Sugiura S, Nishikawa Y, Omata F, Shimazu Y, Kawamura T, Sugiyama A, Nakayama A, Kaneko Y, Kodama T, Kami M, Tsubokura M

    Vaccines (Vaccines)  11 ( 5 ) 920 2023.04

    Research paper (scientific journal), Joint Work, Accepted

     View Summary

    Booster vaccination reduces the incidence of severe cases and mortality related to COVID-19, with cellular immunity playing an important role. However, little is known about the proportion of the population that has achieved cellular immunity after booster vaccination. Thus, we conducted a Fukushima cohort database and assessed humoral and cellular immunity in 2526 residents and healthcare workers in Fukushima Prefecture in Japan through continuous blood collection every 3 months from September 2021. We identified the proportion of people with induced cellular immunity after booster vaccination using the T-SPOT.COVID test, and analyzed their background characteristics. Among 1089 participants, 64.3% (700/1089) had reactive cellular immunity after booster vaccination. Multivariable analysis revealed the following independent predictors of reactive cellular immunity: age < 40 years (adjusted odds ratio: 1.81; 95% confidence interval: 1.19–2.75; p-value: 0.005) and adverse reactions after vaccination (1.92, 1.19–3.09, 0.007). Notably, despite IgG(S) and neutralizing antibody titers of ≥500 AU/mL, 33.9% (349/1031) and 33.5% (341/1017) of participants, respectively, did not have reactive cellular immunity. In summary, this is the first study to evaluate cellular immunity at the population level after booster vaccination using the T-SPOT.COVID test, albeit with several limitations. Future studies will need to evaluate previously infected subjects and their T-cell subsets.

  • Clot waveform analysis for perioperative hemostatic monitoring in a hemophilia A patient on emicizumab undergoing liver transplantation

    Awane M, Wakui M, Ozaki Y, KondoY, Oka S, Fujimori Y, Yatabe Y, Arai T, Yamada Y, Hori S, Obara H, Hasegawa Y, Matsushita H

    Clin Chim Acta (Clinica Chimica Acta)  544   117339 2023.04

    Research paper (scientific journal), Joint Work, Lead author, Corresponding author, Accepted,  ISSN  00098981

     View Summary

    How to optimize perioperative factor VIII (FVIII) replacement through hemostatic monitoring is critically important to manage hemophilia A patients. The bispecific antibody emicizumab binds activated FIX (FIXa) and FX to functionally mimic FVIIIa. While being used for hemostatic control in hemophilia A, this therapeutic antibody inconveniently interferes with coagulation tests using human FIXa and FX, such as activated partial thromboplastin time (APTT) and FVIII activity measurement based on one-stage clotting assays. Clot waveform analysis (CWA) extends the interpretation of measurement curves for coagulation time to provide global information. We performed APTT-CWA to monitor perioperative hemostasis in a hemophilia A patient on emicizumab undergoing liver transplantation. Plasma samples were treated with anti-idiotype monoclonal antibodies against emicizumab to enable accurate coagulation assays. Kinetics of maximum coagulation velocity and acceleration mimicked that of FVIII activity. These CWA parameters better correlated with FVIII activity than APTT. The plateaus of them were observed at FVIII activity of 100% or more, supporting the protocol for perioperative FVIII replacement. Thus, CWA may measure coagulation potential in hemophilia A patients undergoing liver transplantation, aiding in optimizing perioperative hemostasis.

  • 凝固カスケード反応の本質に迫る! 凝固波形解析

    涌井昌俊

    臨床検査専門医会誌(Laboratory and Clinical Practice; Lab Clin Pract) 40 ( 2 ) 36 - 41 2023.03

    Research paper (scientific journal), Single Work

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • Editorial

    涌井昌俊

    血栓止血誌 34 ( 1 ) 3 - 3 2023.02

    Rapid communication, short report, research note, etc. (scientific journal), Single Work, Lead author, Corresponding author

  • ジャーナルクラブ:Platelets mediate inflammatory monocyte activation by SARS-CoV-2 spike protein(SARS-CoV-2スパイクタンパクによって血小板は炎症性単球の活性化をもたらす)

    涌井昌俊

    血栓止血誌 34 ( 3 ) 399 - 402 2023

    Article, review, commentary, editorial, etc. (scientific journal), Joint Work

  • ジャーナルクラブ:Platelets enhance CD4+ central memory T cell responses via platelet factor 4-dependent mitochondrial biogenesis and cell proliferation(血小板は血小板第4因子に依存するミトコンドリア生合成と細胞増殖を介してCD4+ セントラルメモリーT細胞応答を増強する)

    涌井昌俊

    (血栓止血誌)  33 ( 6 ) 736 - 739 2022.12

    Rapid communication, short report, research note, etc. (scientific journal), Single Work

     View Summary

    論文紹介

  • ACB論文紹介5:種々の定量的抗体検査を用いたCOVID-19の液性免疫応答の解析

    涌井昌俊

    臨床化学 51 ( 3 ) 261 - 261 2022.07

    Rapid communication, short report, research note, etc. (scientific journal), Single Work

     View Summary

    論文紹介

  • 出血性素因

    涌井 昌俊

    臨床検査 (医学書院)  印刷中 2019

    Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media), Single Work

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Presentations 【 Display / hide

  • 臨床検査の学際的貢献

    涌井昌俊

    第27回日本心療内科学会総会・学術集会 (東京) , 

    2023.12

    Oral presentation (invited, special), 日本心療内科学会

  • 診療検査データを利用した凝固波形解析の実臨床への応用のための基礎検討

    藤森祐多、涌井昌俊、岡周作、長田恵美里、尾﨑裕子、中村祥子、中川央充、荒井智子、横田浩充、松下弘道

    第70回日本臨床検査医学会学術集会 (長崎) , 

    2023.11

    Oral presentation (general), 日本臨床検査医学会

  • ループス抗凝固因子キットLA試薬 DRVVTによる測定へのDOACおよびヘパリンの影響

    岡周作、涌井昌俊、五十嵐好、矢作かおり、藤森祐多、中村祥子、尾﨑裕子、長田恵美里、近藤佳乃、中川央充、松下弘道

    第70回日本臨床検査医学会学術集会 (長崎) , 

    2023.11

    Oral presentation (general), 日本臨床検査医学会

  • 副甲状腺ホルモン分解における直接経口抗凝固薬によるトロンビンおよび内在性酵素の抑制の意義に関する検討

    村松翔太、岡周作、藤森祐多、中川央充、横田里紗、藏藤利暢、中村祥子、近藤佳乃、尾崎裕子、野口昌代、大野明美、横田浩充、涌井昌俊、松下弘道

    第63回日本臨床化学会年次学術集会 (東京) , 

    2023.10

    Poster presentation, 日本臨床化学会

  • COVID-19に対する抗ウイルス剤の併用により血中タクロリムス濃度が急激に上昇した一例

    新井田凪、中川央充、青木絵美、野口昌代、酒井昭子、大野明美、横田浩充、涌井昌俊、松下弘道

    日本医療検査科学会第55回大会 (横浜) , 

    2023.10

    Oral presentation (general), 日本医療検査科学会

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • 凝固波形解析の自動診断化:血液凝固異常診療のデジタルトランスフォーメーション

    2023.04
    -
    2026.03

    独立行政法人日本学術振興会, 科学研究費助成事業(学術研究助成基金助成金), 基盤研究(C), Research grant, Principal investigator

  • 尿中非抱合型ステロイドによる21水酸化酵素欠損症鑑別法の確立

    2023
    -
    Present

    一般社団法人日本臨床検査医学会, 学術推進プロジェクト研究助成, Other, Coinvestigator(s)

  • 人工知能を用いた凝固検査における直接阻害型経口抗凝固薬血中濃度の指標の探索

    2022.04
    -
    2025.03

    独立行政法人日本学術振興会, 科学研究費補助金, 基盤研究(C), Research grant, Coinvestigator(s)

  • COVID-19重症化を特徴付ける早期免疫代謝の解明と予後マーカーの探索

    2022.04
    -
    2025.03

    独立行政法人日本学術振興会, 科学研究費補助金, 基盤研究(B), Research grant, Coinvestigator(s)

  • 日本の医療従事者におけるCOVID-19ワクチンの液性・細胞性免疫応答と副反応の研究

    2021
    -
    Present

    公益財団法人予防接種リサーチセンター, 公益財団法人予防接種リサーチセンター研究費事業, No Setting, Coinvestigator(s)

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Intellectual Property Rights, etc. 【 Display / hide

  • 質量分析データ処理方法及び装置(分割出願)

    Date applied: 特願2016-074892  2016.04 

    Date announced: 特開2016-153798  2016.08 

    Date issued: 特許第6207036号  2017.09

    Patent, Joint

  • MASS-ANALYSYS DATA PROCESSING METHOD AND SYSTEM

    Date applied: US. Patent Application NO.13/458,850  2012.04 

    Date announced: US. Patent Application Publication NO.US2012/0278037 A1  2012.11 

    Date issued: 特許番号10012572  2018.07

    Patent, Joint

  • 質量分析データ処理方法及び装置

    Date applied: 特願2012-102636  2012.04 

    Date announced: 特開2012-237753  2012.12 

    Date issued: 特許第6025141号  2016.10

    Patent, Joint

  • 免疫応答修飾活性の試験管内評価方法

    Date applied: JP2005019149  2005.10 

    Date announced: WO2006-054415  2006.05 

    Date issued: 4886521  2011.12

    Patent, Joint

  • 移植片対宿主病の早期診断用オリゴヌクレオチド塩基配列

    Date applied: 特願2000-324400  2000.10 

    Date announced: 特開2002-125679  2002.05 

    Patent, Joint

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Other 【 Display / hide

  • peer-review

     View Details

    論文査読(日本臨床検査医学会誌(旧・臨床病理))

  • peer-review

     View Details

    peer-review (International Journal of Hematology)

  • peer-review

     View Details

    論文査読(日本検査血液学会雑誌)

  • peer-review

     View Details

    peer-review (International Journal of Hematology)

  • peer-review

     View Details

    peer-review (Keio Journal of Medicine)

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Courses Taught 【 Display / hide

  • ADVANCED MEDICAL TECHNOLOGIES

    2023

  • PATHOPHYSIOLOGICAL ISSUES IN ACUTE CARE

    2023

  • LECTURE SERIES, LABORATORY MEDICINE

    2023

  • LECTURE SERIES, LABORATORY MEDICINE

    2022

  • ADVANCED MEDICAL TECHNOLOGIES

    2022

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Courses Previously Taught 【 Display / hide

  • 臨床検査学

    Keio University

    2018.04
    -
    2019.03

    Full academic year, Laboratory work/practical work/exercise

  • 臨床検査学

    Keio University

    2018.04
    -
    2019.03

    Autumn Semester, Seminar

  • 実験動物医学

    Keio University

    2018.04
    -
    2019.03

    Spring Semester, Lecture

  • 基礎腫瘍学

    Keio University

    2018.04
    -
    2019.03

    Spring Semester, Lecture

  • 臨床検査学

    Keio University

    2018.04
    -
    2019.03

    Spring Semester, Lecture, Within own faculty

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Educational Activities and Special Notes 【 Display / hide

  • OSCE内部評価者

    2020
    -
    Present

    , Special Affairs

  • 医療系三学部合同教育

    2018
    -
    Present

    , Special Affairs

  • 病院での臨床検査. 第1版, 東京:慶應義塾大学薬学部

    2013

    , Development of Textbook and Teaching Material

  • 医学部カリキュラム委員会

    2010
    -
    2014

    , Special Affairs

  • 医学部入試試験監督

    2010

    , Special Affairs

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Social Activities 【 Display / hide

  • 日本臨床検査専門学院第46期(令和3年度)血液学コースカリキュラム

    一般社団法人 日本衛生検査所協会

    2021
    -
    Present
  • 『臨床検査』誌編集委員

    医学書院

    2019.11
    -
    Present
  • 2019年度慶應連合三田会大会実行委員(救護部会)

    2019年度慶應連合三田会大会実行委員会, 2019年度慶應連合三田会大会 (慶應義塾大学 日吉キャンパス)

    2019.10
  • 『臨床検査』誌ゲストエディター

    医学書院, 臨床検査, 

    2019
    -
    Present
  • Craif社(旧Icaria社) 社外アドバイザー(臨床分野)

    2018.12
    -
    Present

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Media Coverage 【 Display / hide

  • APAC Customer Focus Group Session

    Roche Diagnostics International Ltd,  (Online) , 2021.07

Memberships in Academic Societies 【 Display / hide

  • 日本臨床化学会, 

    2019.01
    -
    Present
  • 日本臨床検査専門医会, 

    2018.01
    -
    Present
  • 日本医用マススペクトル学会, 

    2018
    -
    Present
  • International Society of Laboratory Hematology, 

    2016
    -
    Present
  • 慶應医学会, 

    1989
    -
    Present

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Committee Experiences 【 Display / hide

  • 2023.10

    ワークショップ座長, 第64回日本脈管学会学術総会

  • 2023.10
    -
    Present

    保険診療推進・検査標準化委員会委員長, 日本医用マススペクトル学会

  • 2023.10
    -
    Present

    編集委員会委員, 日本医用マススペクトル学会

  • 2023.10
    -
    Present

    理事, 日本医用マススペクトル学会

  • 2023.09
    -
    Present

    代議員, 日本血栓止血学会

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