Wakui, Masatoshi

写真a

Affiliation

School of Medicine, Department of Laboratory Medicine (Shinanomachi)

Position

Assistant Professor/Senior Assistant Professor

Other Affiliation 【 Display / hide

  • (兼務所属)慶應義塾大学病院臨床研究推進センター, 再生医療等支援部門, センター員

Career 【 Display / hide

  • 1989
    -
    1991

    慶應義塾大学医学部研修医(内科)

  • 1991
    -
    1993

    慶應義塾大学医学部助手(専修医)(内科学)

  • 1991
    -
    1993

    横浜市立市民病院内科

  • 1993
    -
    1995

    慶應義塾大学医学部内科学教室血液研究室(専修医)

  • 1995
    -
    1997

    慶應義塾大学助手(医学部内科学)

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Academic Background 【 Display / hide

  • 1983.04
    -
    1989.03

    Keio University, School of Medicine, 医学科

    Japan, University, Graduated

Academic Degrees 【 Display / hide

  • 博士(医学), Keio University, Dissertation, 2006.09

    Genetic dissection of systemic lupus erythematosus pathogenesis : partial functional complementation between Sle1 and Sle3/5 demonstrates requirement for intracellular coexpression for full phenotypic expression of lupus(全身性エリテマトーデスの成因に関する遺伝学的解析 : Sle1とSle3/5の部分的機能補完は、完全なループス表現型発現のために細胞内共発現が要求されることを示す)

Licenses and Qualifications 【 Display / hide

  • 臨床研修指導医, 第16回慶應義塾大学病院臨床研修指導医養成WS受講

  • 医師免許, 1989.05

  • 臨床検査専門医, 2017.09

  • 臨床検査管理医, 2017.12

  • 日本血栓止血学会認定医, 2020

 

Research Areas 【 Display / hide

  • Tumor biology

  • Tumor diagnostics

  • Medical genome science

  • Pathological medical chemistry

  • Immunology

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Books 【 Display / hide

  • 臨床検査ガイド2020〜2021

    2019,  Page: 印刷中

  • 看護に役立つ検査値の読み方・考え方

    WAKUI Masatoshi, 総合医学社, 2015

  • 臨床検査ガイド2015〜2016

    WAKUI Masatoshi, 文光堂, 2015

  • 検査値の読み方・考え方〜専門医からのアドバイス

    WAKUI Masatoshi, 総合医学社, 2014

  • 病院での臨床検査

    WAKUI Masatoshi, 慶應義塾大学薬学部, 2013

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Papers 【 Display / hide

  • Expertises required in laboratory medicine that contributes to medical research and care becoming diversified

    Masatoshi Wakui

    Rinsho Byori (日本臨床検査医学会)  68 ( 6 ) 535 - 540 2020.06

    Research paper (scientific journal), Single Work, Except for reviews

  • Application of clot-fibrinolysis waveform analysis to assessment of in vitro effects of direct oral anticoagulants on fibrinolysis

    Oka S, Wakui M, Fujimori Y, Kuroda Y, Nakamura S, Kondo Y, Nakagawa T, Katagiri H, Murata M

    Int J Lab Hematol (International Journal of Laboratory Hematology)  42 ( 3 ) 292 - 298 2020.06

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  17515521

     View Summary

    © 2020 John Wiley & Sons Ltd Introduction: Acceleration of fibrinolysis by direct oral anticoagulants (DOACs) has been reported by several groups, suggesting contribution of not only anticoagulant but also fibrinolytic effects to the therapeutic efficacy. The present study aims to evaluate the usability of clot-fibrinolysis waveform analysis (CFWA) for assessment of in vitro effects of DOACs on fibrinolysis. Methods: The experimental conditions were optimized according to how t-PA concentrations and a time length after t-PA adjustment affect parameters of CFWA. Addition of the activated partial thromboplastin time (APTT) reagent followed by that of calcium and t-PA was done to obtain clotting and fibrinolytic reaction curves which were mathematically differentiated for CFWA (APTT-CFWA). The positive and negative modes of waveforms were defined as the direction toward fibrin generation and that toward fibrin degradation, respectively. The maximum positive and negative values (Maxp1 and Maxn1) correspond to the maximum coagulation velocity and the maximum fibrinolysis velocity, respectively. Plasma spiked with each of DOACs (rivaroxaban, apixaban, edoxaban, and dabigatran) was subjected to APTT-CFWA. Results: Optimization of t-PA use was based on Maxn1. Roughly biphasic effects of rivaroxaban and dabigatran but not apixaban or edoxaban on fibrinolysis were observed through Maxn1 and the fibrinolysis peak time, which was defined as a time length from the time when Maxp1 (Maxp1 time) to the time when Maxn1 appears (Maxn1 time). Conclusion: The results suggest the usability of CFWA for assessment of DOAC effects and provide insights into relevance of anticoagulation to therapeutic efficacy and bleeding risk from the perspective of fibrinolysis.

  • CWAによる基礎的検討

    涌井昌俊

    Thrombosis Medicine (先端医学社)  10 ( 1 ) 45 - 54 2020.03

    Research paper (other science council materials etc.), Single Work, Except for reviews

  • Effect of refrigeration of blood samples in lithium-heparin tubes on QuantiFERON TB Gold Plus test result

    Uwamino Y, Sakai A, Nishimura T, Noguchi M, Uno S, Fujiwara H Mori M, Wakui M, Murata M, Hasegawa N

    J Infect Chemother (Journal of Infection and Chemotherapy)  26 ( 3 ) 312 - 314 2020.03

    Research paper (scientific journal), Joint Work, Accepted,  ISSN  1341321X

     View Summary

    © 2019 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases The QuantiFERON TB Gold Plus (QFT-Plus) test is a newly approved interferon-gamma releasing assay test for detecting latent tuberculosis. Although blood samples for QFT test can be refrigerated for 48 h in lithium-heparin tubes according to package inserts, no published data are available on the effects of sample refrigeration on the test results. We conducted a clinical study that aimed to elucidate whether sample refrigeration for 48 h affects QFT-Plus test results. We collected 2 blood samples each from 40 participants for QFT-Plus; one sample was refrigerated before incubation for QFT-Plus assay, while the other sample was incubated soon after collection and treated as control. After comparing QFT-Plus test results of refrigerated samples and control samples, the concordance rate and kappa coefficient between them were 95% and 0.90, respectively. Thus, blood samples for QFT-Plus test can be refrigerated for 48 h in lithium-heparin tubes without influencing the test results.

  • 臨床検査における質量分析法の可能性と落とし穴

    涌井昌俊、中川央充

    臨床検査 (医学書院)  64 ( 3 ) 262 - 271 2020.03

    Research paper (scientific journal), Joint Work, Except for reviews

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Papers, etc., Registered in KOARA 【 Display / hide

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Reviews, Commentaries, etc. 【 Display / hide

  • 出血性素因

    涌井 昌俊

    臨床検査 (医学書院)  印刷中 2019

    Introduction and explanation (commerce magazine), Single Work

  • 静脈血栓症と臨床検査

    藤森祐多、涌井昌俊、村田満

    カレントテラピー (ライフメディコム社)  印刷中 2019

    Introduction and explanation (commerce magazine), Joint Work

  • いま問われる「学問の独立」:大学を取りまく社会と学問(座談会)

    WAKUI Masatoshi

    三田評論 (慶應義塾大学出版会)   ( 1195 ) 10 - 24 2015.12

    Other article, Joint Work

Presentations 【 Display / hide

  • Characterization of degradation of serum parathyroid hormone by proteases

    Nakagawa T, Wakui M, Yokota R, Kurafuji T, Muramatsu S, Fujimori Y, Nakamura S, Kondo Y, Ozaki Y, Oka S, Homma K, Ohta A, Noguchi M, Katagiri H, Ohno A, Murata M

    The 72nd American Association for Clinical Chemistry (AACC) Annual Scientific Meeting (Chicago, Illinois) , 2020.12, Poster (general), American Association for Clinical Chemistry (AACC)

  • 急速凝固採血管内に含まれるトロンビン量と副甲状腺ホルモンの分解速度の関係

    村松翔太、涌井昌俊、横田里紗、藏藤利暢、中川央充、藤森祐多、中村祥子、近藤佳乃、尾崎裕子、岡周作、本間桂子、野口昌代、片桐尚子、大野明美、村田満

    第60回日本臨床化学会年次学術集会 (東京) , 2020.11, Poster (general), 日本臨床化学会

  • FXⅢ欠乏血漿を用いた凝固線溶波形解析に基づく直接トロンビン阻害薬の線溶への影響に関する検討

    岡周作、涌井昌俊、藤森祐多、尾﨑裕子、中村祥子、近藤佳乃、中川央充、片桐尚子、村田満

    第67回日本臨床検査医学会学術集会 (盛岡) , 2020.11, Oral Presentation(general), 日本臨床検査医学会

  • DOAC-Stop処理によるAPTTへの影響と薬剤除去に関する基礎的検討(2020年度優秀演題賞)

    尾﨑裕子、涌井昌俊、藤森祐多、近藤佳乃、岡周作、中村祥子、中川央充、天神卓郎、片桐尚子、村田満

    日本医療検査科学会第52回大会 (横浜) , 2020.09, Oral Presentation(general), 日本医療検査科学会

     View Summary

    2020年度優秀演題賞

  • 当検査室における造血器腫瘍マーカー検査マルチカラーへの取り組み

    荒木美香、谷田部陽子、中村祥子、矢作かおり、森勇人、荒井智子、大野明美、横田浩充、三ツ橋雄之、涌井昌俊、村田満.

    日本医療検査科学会第52回大会 (横浜) , 2020.09, Oral Presentation(general), 日本医療検査科学会

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Exploration into the nature of direct oral anticoagulants (DOACs) from the perspective of laboratory hematology and its application to the management of bleeding risk on medication

    2017.04
    -
    2019.03

    (株)ブリストル・マイヤーズ スクイブ/(株)ファイザー, JRISTA, Masatoshi Wakui, M.D., Ph.D., Principal Investigator

  • 悪性胸膜中皮腫に対するゾレドロン酸によるオートファジー誘導薬物療法

    2014.04
    -
    2017.03

    独立行政法人日本学術振興会, Grant-in-Aid for Scientific Research, 朝倉啓介, No Setting, Co-investigator

  • 遺伝子発現エントロピー解析による腫瘍不均一性の検証

    2013.04
    -
    2016.03

    Grant-in-Aid for Scientific Research, 涌井昌俊, No Setting, Principal Investigator

  • イメージングマススペクトロメトリーのための統計解析法の開発

    2012.04
    -
    2015.03

    Grant-in-Aid for Scientific Research, 公益財団法人 がん研究会・松浦正明, No Setting, Co-investigator

  • 顕微質量分析法による代謝物分布情報に基づく腫瘍不均一性の検証

    2010.04
    -
    2013.03

    Grant-in-Aid for Scientific Research, 涌井昌俊, No Setting, Principal Investigator

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Intellectual Property Rights, etc. 【 Display / hide

  • 質量分析データ処理方法及び装置(分割出願)

    Application No.: 特願2016-074892  2016.04 

    Announcement No.: 特開2016-153798  2016.08 

    Registration No.: 特許第6207036号  2017.09

    Patent, Joint, National application

  • MASS-ANALYSYS DATA PROCESSING METHOD AND SYSTEM

    Application No.: US. Patent Application NO.13/458,850  2012.04 

    Announcement No.: US. Patent Application Publication NO.US2012/0278037 A1  2012.11 

    Registration No.: 特許番号10012572  2018.07

    Patent, Joint, PCT international application

  • 質量分析データ処理方法及び装置

    Application No.: 特願2012-102636  2012.04 

    Announcement No.: 特開2012-237753  2012.12 

    Registration No.: 特許第6025141号  2016.10

    Patent, Joint, National application

  • 免疫応答修飾活性の試験管内評価方法

    Application No.: JP2005019149  2005.10 

    Announcement No.: WO2006-054415  2006.05 

    Registration No.: 4886521  2011.12

    Patent, Joint

  • 移植片対宿主病の早期診断用オリゴヌクレオチド塩基配列

    Application No.: 特願2000-324400  2000.10 

    Announcement No.: 特開2002-125679  2002.05 

    Patent, Joint, National application

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Other 【 Display / hide

  • peer-review

     View Details

    peer-review (International Journal of Hematology)

  • peer-review

     View Details

    peer-review (International Journal of Hematology)

  • peer-review

     View Details

    peer-review (International Journal of Hematology)

  • peer-review

     View Details

    peer-review (PLOS ONE)

  • peer-review

     View Details

    peer-review (Scientific Reports)

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Courses Taught 【 Display / hide

  • 生化学講義(早稲田大学 先進理工学部)

    2020, Lecture

  • 臨床検査学臨床実習(医学部)

    2020, Laboratory work/practical work/exercise

  • LECTURE SERIES, LABORATORY MEDICINE

    2020

  • 基礎腫瘍学(大学院博士課程)

    2020, Other, Lecture

  • 動物実験医学(大学院博士課程)

    2020, Other

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Courses Previously Taught 【 Display / hide

  • 臨床検査学

    Keio University, 2018, Spring Semester, Major subject, Lecture, Within own faculty

  • 臨床検査学

    Keio University, 2018, Full academic year, Laboratory work/practical work/exercise

  • 臨床検査学

    Keio University, 2018, Autumn Semester, Seminar

  • 実験動物医学

    Keio University, 2018, Spring Semester, Lecture

  • 基礎腫瘍学

    Keio University, 2018, Spring Semester, Major subject, Lecture

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Educational Activities and Special Notes 【 Display / hide

  • OSCE内部評価者

    2020
    -
    Present

    , Special Affairs

  • 医療系三学部合同教育

    2018
    -
    Present

    , Special Affairs

  • 病院での臨床検査. 第1版, 東京:慶應義塾大学薬学部

    2013

    , Development of Textbook and Teaching Material

  • 医学部カリキュラム委員会

    2010
    -
    2014

    , Special Affairs

  • 医学部入試試験監督

    2010

    , Special Affairs

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Social Activities 【 Display / hide

  • 『臨床検査』誌編集委員

    医学書院

    2019.11
    -
    Present
  • 2019年度慶應連合三田会大会実行委員(救護部会)

    2019年度慶應連合三田会大会実行委員会, 2019年度慶應連合三田会大会 (慶應義塾大学 日吉キャンパス)

    2019.10
  • 『臨床検査』誌ゲストエディター

    医学書院, 臨床検査, 

    2019
    -
    Present
  • Craif社(旧Icaria社) 社外アドバイザー(臨床分野)

    2018.12
    -
    Present
  • 健康医療ベンチャー大賞(慶應義塾大学医学部主催、理工学部・慶應ビジネススクール共催)医療従事者対象ヒアリング協力

    2018
    -
    Present

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Memberships in Academic Societies 【 Display / hide

  • 日本臨床化学会, 

    2019.01
    -
    Present
  • 日本臨床検査専門医会, 

    2018.01
    -
    Present
  • 日本医用マススペクトル学会, 

    2018
    -
    Present
  • International Society of Laboratory Hematology, 

    2016
    -
    Present
  • 慶應医学会, 

    1989
    -
    Present

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Committee Experiences 【 Display / hide

  • 2020.10

    一般演題(口演)座長, 第60回日本臨床化学会年次学術集会

  • 2020.09
    -
    Present

    評議員, 日本医用マススペクトル学会

  • 2020.09

    一般演題(口演)座長 , 日本医療検査科学会第52回大会

  • 2020.06

    一般演題(口演)座長, 第42回日本血栓止血学会学術集会

  • 2020.01
    -
    Present

    日本臨床検査専門医会 教育研修委員会 委員, 日本臨床検査専門医会

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