Uwamino, Yoshifumi

写真a

Affiliation

School of Medicine, Department of Laboratory Medicine （Shinanomachi）

Position

Senior Assistant Professor (Non-tenured)/Assistant Professor (Non-tenured)
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Career 【 Display / hide 】

  • 2007.04
    -
    2009.03

    東京都済生会中央病院, 初期臨床研修医

  • 2009.04
    -
    2011.03

    東京都済生会中央病院, 一般内科, 専修医

  • 2011.04
    -
    2014.03

    鉄蕉会亀田総合病院, 総合診療・感染症科, 感染症フェロー

  • 2014.04
    -
    2016.03

    慶應義塾大学医学部, 感染制御センター, 助教

  • 2016.04
    -
    2020.09

    慶應義塾大学医学部, 臨床検査医学, 助教

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Academic Background 【 Display / hide 】

  • 2001.04
    -
    2007.03

    Keio University, 医学部

    University, Graduated

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Academic Degrees 【 Display / hide 】

  • Ph.D. in Medicine, Keio University, Dissertation, 2020.07

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Licenses and Qualifications 【 Display / hide 】

  • 臨床研修指導医講習会修了

  • 日本臨床検査医学会臨床検査管理医

  • 日本臨床検査医学会臨床検査専門医

  • 日本感染症学会感染症専門医

  • 日本内科学会総合内科専門医

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Papers 【 Display / hide 】

  • Nationwide surveillance of bacterial respiratory pathogens conducted by the surveillance committee of the Japanese Society of Chemotherapy, the Japanese Association for Infectious Diseases, and the Japanese Society for Clinical Microbiology in 2019–2020: General view of the pathogens' antibacterial susceptibility

    Tokimatsu I., Matsumoto T., Tsukada H., Fujikura Y., Miki M., Morinaga Y., Sato J., Wakamura T., Kiyota H., Tateda K., Yanagisawa H., Sasaki T., Ikeda H., Horikawa H., Takahashi H., Seki M., Mori Y., Takeda H., Kurai D., Hasegawa N., Uwamino Y., Kudo M., Yamamoto M., Nagano Y., Nomura S., Tetsuka T., Hosokai M., Aoki N., Yamamoto Y., Iinuma Y., Mikamo H., Suematsu H., Maruyama T., Kawabata A., Sugaki Y., Nakamura A., Fujikawa Y., Fukumori T., Ukimura A., Kakeya H., Niki M., Yoshida K., Kobashi Y., Tokuyasu H., Yatera K., Ikegami H., Fujita M., Matsumoto T., Yanagihara K., Matsuda J., Hiramatsu K., Shinzato T.

    Journal of Infection and Chemotherapy （Journal of Infection and Chemotherapy)  29 （ 8 ） 731 - 743 2023.08

    ISSN  1341321X

    　View Summary

    The trends and prevalence of antimicrobial susceptibility of pathogens vary by country, region, and time. Long-term regular surveillance is required to investigate trends in the antimicrobial resistance of various isolated bacterial pathogens. We report the results of a nationwide surveillance on the antimicrobial susceptibility of bacterial respiratory pathogens in Japan conducted by the Japanese Society of Chemotherapy, the Japanese Association for Infectious Diseases, and the Japanese Society for Clinical Microbiology. The isolates were collected from clinical specimens obtained from adult patients who visited a collaborating medical facility between June 2019 and December 2020 and were diagnosed with respiratory tract infections by a physician. Antimicrobial susceptibility testing was performed in a centralized laboratory according to the methods recommended by the Clinical and Laboratory Standards Institute. Susceptibility testing was performed for 932 strains (201 Staphylococcus aureus, 158 Streptococcus pneumoniae, 6 S. pyogenes, 136 Haemophilus influenzae, 127 Moraxella catarrhalis, 141 Klebsiella pneumoniae, and 163 Pseudomonas aeruginosa) collected from 32 facilities in Japan. The proportions of methicillin-resistant S. aureus and penicillin-resistant S. pneumoniae were 35.3% and 0%, respectively. In H. influenzae, 16.2% and 16.9% were β-lactamase-producing ampicillin resistant and β-lactamase-negative ampicillin resistant, respectively. Extended-spectrum β-lactamase-producing K. pneumoniae accounted for 5.0% of all K. pneumoniae infections. Carbapenemase-producing K. pneumoniae and multi-drug-resistant P. aeruginosa with metallo-β-lactamase were not detected in this study. This surveillance will be a useful reference for treating respiratory infections in Japan and will provide evidence to enhance the appropriate use of antimicrobial agents.

  • Differences among epitopes recognized by neutralizing antibodies induced by SARS-CoV-2 infection or COVID-19 vaccination

    Yamamoto S., Yamayoshi S., Ito M., Sakai-Tagawa Y., Nakachi I., Baba R., Kamimoto S., Ogura T., Hagiwara S., Kato H., Nakajima H., Uwamino Y., Yagi K., Sugaya N., Nagai H., Saito M., Adachi E., Koga M., Tsutsumi T., Duong C., Okuda M., Murakami J., Furusawa Y., Ujie M., Iwatsuki-Horimoto K., Yotsuyanagi H., Kawaoka Y.

    iScience （iScience)  26 （ 7 ） 107208 2023.07

    　View Summary

    SARS-CoV-2 has gradually acquired amino acid substitutions in its S protein that reduce the potency of neutralizing antibodies, leading to decreased vaccine efficacy. Here, we attempted to obtain mutant viruses by passaging SARS-CoV-2 in the presence of plasma samples from convalescent patients or vaccinees to determine which amino acid substitutions affect the antigenicity of SARS-CoV-2. Several amino acid substitutions in the S2 region, as well as the N-terminal domain (NTD) and receptor-binding domain (RBD), affected the neutralization potency of plasma samples collected from vaccinees, indicating that amino acid substitutions in the S2 region as well as those in the NTD and RBD affect neutralization by vaccine-induced antibodies. Furthermore, the neutralizing potency of vaccinee plasma samples against mutant viruses we obtained or circulating viruses differed among individuals. These findings suggest that genetic backgrounds of vaccinees influence the recognition of neutralizing epitopes.

  • Healthcare costs for hospitalized COVID-19 patients in a Japanese university hospital: a cross-sectional study.

    Uno S, Goto R, Honda K, Tokuda M, Kamata H, Chubachi S, Yamamoto R, Sato Y, Homma K, Uchida S, Namkoong H, Uwamino Y, Sasaki J, Fukunaga K, Hasegawa N

    Cost effectiveness and resource allocation : C/E 21 （ 1 ） 43 2023.07

    ISSN  1478-7547

  • Single-cell analyses and host genetics highlight the role of innate immune cells in COVID-19 severity

    Edahiro R., Shirai Y., Takeshima Y., Sakakibara S., Yamaguchi Y., Murakami T., Morita T., Kato Y., Liu Y.C., Motooka D., Naito Y., Takuwa A., Sugihara F., Tanaka K., Wing J.B., Sonehara K., Tomofuji Y., Wang Q.S., Hasegawa T., Saiki R., Hyugaji T., Shimizu E., Katayama K., Kanai M., Naito T., Sasa N., Yamamoto K., Takahashi K., Harada N., Naito T., Hiki M., Matsushita Y., Takagi H., Ichikawa M., Nakamura A., Harada S., Sandhu Y., Kabata H., Masaki K., Kamata H., Ikemura S., Chubachi S., Okamori S., Terai H., Morita A., Asakura T., Sasaki J., Morisaki H., Uwamino Y., Nanki K., Uchida S., Uno S., Nishimura T., Ishiguro T., Isono T., Shibata S., Matsui Y., Hosoda C., Takano K., Nishida T., Kobayashi Y., Takaku Y., Takayanagi N., Ueda S., Tada A., Miyawaki M., Yamamoto M., Yoshida E., Hayashi R., Nagasaka T., Arai S., Kaneko Y., Sasaki K., Tagaya E., Kawana M., Arimura K., Takahashi K., Anzai T., Ito S., Endo A., Uchimura Y., Miyazaki Y., Honda T., Tateishi T., Tohda S., Ichimura N., Sonobe K., Sassa C.T., Nakajima J., Nakano Y., Nakajima Y., Anan R., Arai R., Kurihara Y., Harada Y., Nishio K., Ueda T., Azuma M., Saito R., Sado T.

    Nature Genetics （Nature Genetics)  55 （ 5 ） 753 - 767 2023.05

    ISSN  10614036

    　View Summary

    Mechanisms underpinning the dysfunctional immune response in severe acute respiratory syndrome coronavirus 2 infection are elusive. We analyzed single-cell transcriptomes and T and B cell receptors (BCR) of >895,000 peripheral blood mononuclear cells from 73 coronavirus disease 2019 (COVID-19) patients and 75 healthy controls of Japanese ancestry with host genetic data. COVID-19 patients showed a low fraction of nonclassical monocytes (ncMono). We report downregulated cell transitions from classical monocytes to ncMono in COVID-19 with reduced CXCL10 expression in ncMono in severe disease. Cell–cell communication analysis inferred decreased cellular interactions involving ncMono in severe COVID-19. Clonal expansions of BCR were evident in the plasmablasts of patients. Putative disease genes identified by COVID-19 genome-wide association study showed cell type-specific expressions in monocytes and dendritic cells. A COVID-19-associated risk variant at the IFNAR2 locus (rs13050728) had context-specific and monocyte-specific expression quantitative trait loci effects. Our study highlights biological and host genetic involvement of innate immune cells in COVID-19 severity.

  • Diagnostic Utility of a Mycobacterium Multiplex PCR Detection Panel for Tuberculosis and Nontuberculous Mycobacterial Infections.

    Yoshifumi Uwamino, Akio Aono, Yu Tomita, Kozo Morimoto, Masahiro Kawashima, Hirofumi Kamata, Yuka Sasaki, Hideaki Nagai, Naoki Hasegawa, Satoshi Mitarai

    Microbiology spectrum （Microbiology spectrum)  11 （ 3 ） e0516222 2023.04

    　View Summary

    The prevalence of nontuberculous mycobacterial diseases is increasing worldwide, and tuberculosis remains highly prevalent. Rapid and accurate microbial diagnoses of both tuberculosis and nontuberculous mycobacterial infections are required. A novel PCR-reverse sequence-specific oligonucleotide probe (PCR-rSSO) method-based mycobacterial detection panel (Myco-Panel) test was developed for the rapid identification of 30 mycobacterial species and subspecies. Clinical respiratory samples were collected from patients with suspected or confirmed tuberculosis and nontuberculous mycobacterial lung disease and those with other respiratory diseases. Myco-Panel tests were performed on the samples, and liquid mycobacterial culture and identification were performed for reference according to housekeeping gene sequences of mycobacteria in positive culture tubes. Furthermore, to assess the detection performance for several mycobacterial species rarely recovered in Japan, the accuracy of the Myco-Panel test was investigated using stock mycobacterial type strains and clinical isolates. A total of 178 clinical respiratory samples were analyzed. The Myco-Panel and sequence-based identification results for mycobacterial cultures were 83.1% concordant (kappa coefficient, 0.785 [95% confidence interval, 0.716 to 0.854]). The Myco-Panel correctly identified 281 of the 283 type strains and clinical isolates tested. The Myco-Panel test could accurately detect several mycobacterial species from clinical respiratory samples and mycobacterial suspensions. Rapid and accurate identification of pathogens using respiratory samples is possible using the Myco-Panel. IMPORTANCE Species identification is important for the diagnosis of mycobacterial infections and decisions on treatment regimens. The Myco-Panel test accurately detects clinically common mycobacterial species that cause respiratory infections from clinical respiratory samples and mycobacterial suspensions. The rapid identification of multiple mycobacterial species will provide clinically useful information for the management of patients. Although we understand that the current diagnostic criteria require mycobacterial culture results in general for the diagnosis of nontuberculous mycobacterial infection, mycobacterial culture examination is a time-consuming process. The detection of potentially causative agents directly from clinical samples will aid in practical diagnosis and decision-making for rapid treatment initiation. This is a new laboratory method for species identification, and evaluating its performance is important.

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Papers, etc., Registered in KOARA 【 Display / hide 】

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Reviews, Commentaries, etc. 【 Display / hide 】

  • ASTを担当する薬剤師の具体的な活動を共有し,発展させよう!　大学病院におけるAST活動の軌跡を振りカエル(KAERU)

    瀬山 翔史, 池谷 修, 上蓑 義典, 宇野 俊介, 内田 翔, 南宮 湖, 木村 元範, 福田 正悟, 荒 美幸, 尾原 秀明, 新庄 正宜, 高野 八百子, 長谷川 直樹

    日本感染症学会総会・学術講演会・日本化学療法学会学術集会合同学会プログラム・抄録集 (日本感染症学会・日本化学療法学会)  97回・71回   シンポジウム16 - シンポジウム16 2023.03

  • Enterococcus faecalis菌血症において質量分析器における血液培養陽性ボトルからの直接同定法が抗菌薬選択に及ぼす影響

    木村 陽菜, 上蓑 義典, 猪狩 嘉孝, 鮫島 朱里, 加藤 藍, 青木 渉, 石原 治, 永田 美香, 猪瀬 里夏, 宇野 俊介, 南宮 湖, 長谷川 直樹, 松下 弘道

    日本臨床微生物学会雑誌 ((一社)日本臨床微生物学会)  33 ( Suppl.1 ) 266 - 266 2022.12

    ISSN  2434-866X

  • 全自動釣菌装置BDキエストラidentifAを用いた前処理の臨床使用における性能の検討

    猪狩 嘉孝, 上蓑 義典, 木村 陽菜, 鮫島 朱里, 滝本 理絵, 加藤 藍, 青木 渉, 石原 治, 永田 美香, 猪瀬 里夏, 松下 弘道

    日本臨床微生物学会雑誌 ((一社)日本臨床微生物学会)  33 ( Suppl.1 ) 224 - 224 2022.12

    ISSN  2434-866X

  • 【新型コロナとインフルエンザ　同時流行に備えるために再確認しておきたいこと】検査(PART.4)　インフルエンザと新型コロナの検査でおさえておきたいこと

    上蓑 義典

    Expert Nurse ((株)照林社)  38 ( 13 ) 26 - 29 2022.10

    ISSN  0911-0194

  • 一般細菌培養検査での肺外検体における抗酸菌検出に関する後方視的調査

    石原 治, 上蓑 義典, 猪瀬 里夏, 荒井 智子, 横田 浩充, 長谷川 直樹, 松下 弘道

    日本臨床検査医学会誌 ((一社)日本臨床検査医学会)  70 ( 補冊 ) 132 - 132 2022.10

    ISSN  2436-2727

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

  • Utility of sex steroid hormones measurement as biomarker for mycobacterial diseses

    2021.04
    -
    2024.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

  • インターフェロンγ遊離試験を用いた結核感染診断における性周期の影響

    2018.04
    -
    2020.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Early-Career Scientists , Principal investigator

  • Association of sex steroid hormones and non-tuberculous mycobacteriosis

    2016.04
    -
    2018.03

    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Young Scientists (B), Principal investigator

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Courses Taught 【 Display / hide 】

  • PRIMARY HEALTHCARE AND GLOBAL HEALTH II: OVERSEAS STUDY TOUR

    2024

  • PATHOPHYSIOLOGICAL ISSUES IN ACUTE CARE

    2024

  • LECTURE SERIES, LABORATORY MEDICINE

    2024

  • INFECTIOUS DISEASES

    2024

  • PRIMARY HEALTHCARE AND GLOBAL HEALTH II: OVERSEAS STUDY TOUR

    2023

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