森川　暁 （モリカワ　サトル）

Morikawa, Satoru

写真a

所属（所属キャンパス）

医学部 歯科・口腔外科学教室 （信濃町）

職名

専任講師
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経歴 【 表示 ／ 非表示 】

  • 2011年04月
    -
    継続中

    慶應義塾大学医学部 歯科・口腔外科学教室

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学歴 【 表示 ／ 非表示 】

  • 2005年04月
    -
    2009年03月

    慶應義塾大学, 医学研究科博士課程

    大学院, 修了

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学位 【 表示 ／ 非表示 】

  • 博士（医学）, 慶應義塾大学大, 課程

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研究分野 【 表示 ／ 非表示 】

  • ライフサイエンス / 口腔再生医学、歯科医用工学

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研究キーワード 【 表示 ／ 非表示 】

  • 口腔がん

  • 幹細胞

  • 歯周病

  • 組織幹細胞

  • 間葉系幹細胞

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論文 【 表示 ／ 非表示 】

  • Recurrent Spindle Cell Carcinoma Shows Features of Mesenchymal Stem Cells

    Ouchi T., Morikawa S., Shibata S., Takahashi M., Yoshikawa M., Soma T., Miyashita H., Muraoka W., Kameyama K., Kawana H., Arima Y., Saya H., Okano H., Nakagawa T., Asoda S.

    Journal of Dental Research （Journal of Dental Research）  97 （ 7 ） 779 - 786 2018年07月

    ISSN  1544-0591

    　概要を見る

    This study investigated a case of spindle cell carcinoma (SpCC) in tongue pathological lesions. The patient experienced a local recurrence and distant metastasis after surgical intervention. Although standard chemotherapy was administered, a granulomatous mass continued to develop. This aggressive growth led to survival of the tumor. Secondary debulking surgery was performed to improve the patient’s quality of life at the request of the patient. Using a tissue sample derived from the secondary debulking surgery, we performed an analysis of the tumor’s cell surface antigens, differentiation potential, metastatic ability, and inhibition potential by anticancer reagents. In vitro analysis revealed that the cell population grown under adherent culture conditions expressed the mesenchymal stem cell (MSC) markers CD73, CD90, and CD105. The cell line established from this SpCC contained colony-forming unit fibroblasts (CFU-Fs) and exhibited multipotent differentiation into several mesenchymal lineages, including bone, cartilage, and fat. The SpCC cells also displayed vigorous mobilization. These characteristics suggested that they had the differentiation potential of mesenchymal cells, especially MSCs, rather than that of epithelial cells. The surgical specimen analyzed in this study resisted the molecular target reagent cetuximab, which is an epidermal growth factor receptor inhibitor. This clinical insight revealed that chemotherapy-resistant SpCC cells have different characteristics compared to most other cancer cells, which are sensitive to cetuximab. Our cell death assay revealed that SpCC cell death was induced by the anticancer drug imatinib, which is known to inhibit protein tyrosine kinase activity of ABL, platelet-derived growth factor receptor α (PDGFRα), and KIT. Here, we report recurrent SpCC with characteristics of MSCs and potential for treatment with imatinib.

  • Treatment of severe generalized chronic periodontitis in a patient with Behçet’s disease: A case report

    Morikawa S., Ouchi T., Asoda S., Horie N., Tsunoda K., Kawana H., Nakagawa T.

    Journal of International Medical Research （Journal of International Medical Research）  46 （ 5 ） 2037 - 2045 2018年05月

    ISSN  1473-2300

    　概要を見る

    Behçet’s disease is a systemic disorder of unknown etiology. It involves multiple organ systems and is characterized by recurring episodes of oral ulcers as well as ocular, genital, and skin lesions. Oral ulcers can affect tooth brushing and impair proper oral hygiene. As a result, a dental biofilm accumulates, and the condition of the teeth and periodontal tissue deteriorates. The aim of this case report is to highlight the efficacy of periodontal treatment for patients with Behçet’s disease. A 51-year-old man with Behçet’s disease presented with generalized severe periodontitis. After basic treatment of the periodontal tissues, periodontal surgery was performed at several sites with bony defects. However, the patient developed severe stomatitis in the oral mucosa and gingiva after periodontal surgery. Administration of the antimicrobial agent cefdinir had little effect on recovery; however, subsequent administration of sitafloxacin resulted in significant improvement of the stomatitis. This case demonstrates that periodontal therapy is very useful for alleviating the oral signs and symptoms of Behçet’s disease. Systemic antibiotic treatment with sitafloxacin (but not cefdinir) and mechanical debridement were effective in preventing the recurrence of aphthous ulcer outbreaks after periodontal surgery.

  • Functional role of lacrimal gland fibroblasts in a mouse model of chronic graft-versus-host disease

    Yamane M., Ogawa Y., Mukai S., Yaguchi S., Kamijuku H., Inaba T., Asai K., Morikawa S., Kawakami Y., Shimmura S., Tsubota K.

    Cornea （Cornea）  37 （ 1 ） 102 - 108 2018年01月

    ISSN  1536-4798

    　概要を見る

    Purpose: This study aimed to clarify the mechanisms and assess the characteristics of the chronic graft-versus-host disease (cGVHD) fibrosis in the lacrimal gland (LG) of mice. Methods: Histopathology of LG tissues was examined by immunohistochemistry and electron microscopy. Cultured fibroblasts derived from the LG were analyzed by phase-contrast microscopy, immunocytochemistry, flow cytometry, proliferation assay, and invasion and migration assays. Results: Cultured murine LG fibroblasts in cGVHD were spindle-shaped and relatively small, whereas those from syngeneic controls were polygon-shaped and relatively large. Flow cytometric analysis showed that the LG fibroblasts in cGVHD had elevated HSP47 levels. The LG fibroblasts in cGVHD also showed increased expression of major histocompatibility complex class II. Furthermore, the proportion of Sca-1+PDGFR-a+ cells among the LG fibroblasts in cGVHD was considerably increased compared with controls. Cell counting kit-8 assays demonstrated that the LG fibroblasts in cGVHD were highly proliferative, and cell invasion assays indicated that they were highly invasive with high migration ability. Conclusions: LG fibroblasts in cGVHD can be aberrantly activated, thereby eliciting fibrosis by producing excessive extracellular matrix, leading to LG dysfunction in mice.

  • The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry

    Niibe, K., Zhang, M., Nakazawa, K., Morikawa, S., Nakagawa, T., Matsuzaki, Y. and Egusa, H.

    Jpn Dent Sci Rev 53 （ 2 ） 25 - 33 2017年05月

    ISSN  1882-7616

    　概要を見る

    Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRalpha(+) and Sca-1(+)) and humans (LNGFR(+), THY-1(+) and VCAM-1(+)), facilitating the isolation of unique enriched BM-MSCs (so-called "purified MSCs"). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.

  • Isolation of dental pulp stem cells with high osteogenic potential

    Yasui, T., Mabuchi, Y., Morikawa, S., Onizawa, K., Akazawa, C., Nakagawa, T., Okano, H. and Matsuzaki, Y.

    Inflamm Regen 37 （ April ） 8 2017年04月

    ISSN  1880-9693

    　概要を見る

    Dental pulp stem cells/progenitor cells (DPSCs) can be easily obtained and can have excellent proliferative and mineralization potentials. Therefore, many studies have investigated the isolation and bone formation of DPSCs. In most previous reports, human DPSCs were traditionally isolated by exploiting their ability to adhere to plastic tissue culture dishes. DPSCs isolated by plastic adherence are frequently contaminated by other cells, which limits the ability to investigate their basic biology and regenerative properties. Additionally, the proliferative and osteogenic potentials vary depending on the isolated cells. It is very difficult to obtain cells of a sufficient quality to elicit the required effect upon transplantation. Considering clinical applications, stem cells used for regenerative medicine need to be purified in order to increase the efficiency of bone regeneration, and a stable supply of these cells must be generated. Here, we review the purification of DPSCs and studies of cranio-maxillofacial bone regeneration using these cells. Additionally, we introduce the prospective isolation of DPSCs using specific cell surface markers: low-affinity nerve growth factor and thymocyte antigen 1.

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研究発表 【 表示 ／ 非表示 】

  • Full-mouth disinfection, systemic antibacterial therapy, and regenerative periodontal therapy for generalized aggressive periodontitis: a case report

    Morikawa S, Ouchi T, Nakayama R, Nakagawa T.

    EuroPerio9 (9TH CONFERENCE OF THE EUROPEAN FEDERATION OF PERIODONTLOGY) （Amsterdam） , 

    2018年06月
    , 

    ポスター発表, European Federation of Periodontlogy

  • A case report of severe chronic periodontitis with Amlodipine-induced gingival overgrowth

    Nakayama R, Morikawa, S, Nakagawa T.

    EuroPerio9 (9TH CONFERENCE OF THE EUROPEAN FEDERATION OF PERIODONTLOGY) （Amsterdam） , 

    2018年06月
    , 

    ポスター発表, European Federaration of Periodontlogy

  • Identification of mesenchymal stem cells with bright expression of LNGFR and THY-1 in neural crest-like cells derived from human iPS cells

    Ouchi T, Morikawa S, Nakagawa, T.

    EuroPerio9 (9TH CONFERENCE OF THE EUROPEAN FEDERATION OF PERIODONTLOGY) （Amsterdam） , 

    2018年06月
    , 

    ポスター発表, European Federation of Periodontlogy

  • LNGFR(+)THY-1(+) multipotent stem cells derived from human induced pluripotent stem cells

    Ouchi T, Shibata S, Kimura H, Nagoshi N, Fujimura Y, Morikawa, S, Sato K, Kawana H, Fukuda K, Nakamura M, Nakagawa T, Okano H.

    18th International Congress of Developmental Biology （Singapore） , 

    2017年06月
    , 

    ポスター発表

  • Generalized severe chronic periodontitis treated with regenerative therapy

    Morikawa S, Ouchi T, Nakagawa T.

    102nd Annual Meeting of the American Academy of Periodontology （San Diego） , 

    2016年09月
    , 

    ポスター発表, American Academy of Periodontology

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競争的研究費の研究課題 【 表示 ／ 非表示 】

  • CD73(+)MSCs集積機能型歯周炎・インプラント周囲炎抗菌再建デバイスの開発

    2022年04月
    -
    2025年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 森川 暁, 基盤研究(C), 補助金,  研究代表者

  • PDGFRα(+)間葉系幹細胞誘導システムを用いた新規骨置換材料の開発

    2019年04月
    -
    2022年03月

    文部科学省・日本学術振興会, 科学研究費助成事業, 森川　暁, 基盤研究（Ｃ）, 補助金,  研究代表者

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知的財産権等 【 表示 ／ 非表示 】

  • ヒトiPS細胞から、ヒト歯原性上皮細胞やヒト歯原性間葉細胞を製造する方法

    出願日： P2016-073957  2016年04月 

    特許権, 共同

  • ヒト間葉系幹細胞濃縮方法

    出願日： PCT/JP2008/066169 (USA 12/676,827)  2008年09月 

    特許権, 共同

  • ヒト間葉系幹細胞濃縮方法

    出願日： P2007-231298  2007年09月 

    特許権, 共同

  • 分化細胞由来多能性幹細胞の樹立方法

    出願日： 2009181009   

    特許権, 共同

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受賞 【 表示 ／ 非表示 】

  • 2010年11月　慶應義塾大学医学部三四会奨励賞

    2010年11月

  • 第9回日本再生医療学会総会　Young Investigator’s Award（若手研究奨励賞）

    2010年03月

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担当授業科目 【 表示 ／ 非表示 】

  • 歯科学講義

    2024年度

  • 歯科学講義

    2023年度

  • 歯科学講義

    2022年度

  • 歯科学講義

    2021年度

  • 歯科学講義

    2020年度

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担当経験のある授業科目 【 表示 ／ 非表示 】

  • 歯科医学

    慶應義塾

    2018年04月
    -
    2019年03月
    , 

    秋学期, 講義

  • 歯科医学

    慶應義塾

    2017年04月
    -
    2018年03月
    , 

    講義

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所属学協会 【 表示 ／ 非表示 】

  • 日本口腔インプラント学会

    　

  • 日本歯周病学会

    　

  • 日本口腔外科学会

    　

  • 日本口腔科学会

    　

  • 日本再生医療学会

    　
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