Morikawa, Satoru



School of Medicine, Department of Dentistry and Oral Surgery (Shinanomachi)


Assistant Professor/Senior Assistant Professor

Career 【 Display / hide

  • 2011.04

    Department of Dentistry and Oral Surgery, Keio University School of Medicine

Academic Background 【 Display / hide

  • 2005.04

    Keio University, 医学研究科博士課程

    Graduate School, Completed

Academic Degrees 【 Display / hide

  • 博士(医学), 慶應義塾大学大, Coursework


Research Areas 【 Display / hide

  • Life Science / Regenerative dentistry and dental engineering

Research Keywords 【 Display / hide

  • Oral cancer

  • Stem cells

  • Periodontal disease

  • Tissue stem cells

  • Mesenchymal stem cells


Papers 【 Display / hide

  • Recurrent Spindle Cell Carcinoma Shows Features of Mesenchymal Stem Cells

    Ouchi, T., Morikawa, S., Shibata, S., Takahashi, M., Yoshikawa, M., Soma, T., Miyashita, H., Muraoka, W., Kameyama, K., Kawana, H., Arima, Y., Saya, H., Okano, H., Nakagawa, T. and Asoda, S.

    J Dent Res (Journal of Dental Research)  97 ( 7 ) 779 - 786 2018.07

    ISSN  1544-0591

     View Summary

    This study investigated a case of spindle cell carcinoma (SpCC) in tongue pathological lesions. The patient experienced a local recurrence and distant metastasis after surgical intervention. Although standard chemotherapy was administered, a granulomatous mass continued to develop. This aggressive growth led to survival of the tumor. Secondary debulking surgery was performed to improve the patient's quality of life at the request of the patient. Using a tissue sample derived from the secondary debulking surgery, we performed an analysis of the tumor's cell surface antigens, differentiation potential, metastatic ability, and inhibition potential by anticancer reagents. In vitro analysis revealed that the cell population grown under adherent culture conditions expressed the mesenchymal stem cell (MSC) markers CD73, CD90, and CD105. The cell line established from this SpCC contained colony-forming unit fibroblasts (CFU-Fs) and exhibited multipotent differentiation into several mesenchymal lineages, including bone, cartilage, and fat. The SpCC cells also displayed vigorous mobilization. These characteristics suggested that they had the differentiation potential of mesenchymal cells, especially MSCs, rather than that of epithelial cells. The surgical specimen analyzed in this study resisted the molecular target reagent cetuximab, which is an epidermal growth factor receptor inhibitor. This clinical insight revealed that chemotherapy-resistant SpCC cells have different characteristics compared to most other cancer cells, which are sensitive to cetuximab. Our cell death assay revealed that SpCC cell death was induced by the anticancer drug imatinib, which is known to inhibit protein tyrosine kinase activity of ABL, platelet-derived growth factor receptor alpha (PDGFRalpha), and KIT. Here, we report recurrent SpCC with characteristics of MSCs and potential for treatment with imatinib.

  • Treatment of severe generalized chronic periodontitis in a patient with Behcet's disease: A case report

    Morikawa, S., Ouchi, T., Asoda, S., Horie, N., Tsunoda, K., Kawana, H. and Nakagawa, T.

    J Int Med Res (Journal of International Medical Research)  46 ( 5 ) 2037 - 2045 2018.05

    ISSN  1473-2300

     View Summary

    Behcet's disease is a systemic disorder of unknown etiology. It involves multiple organ systems and is characterized by recurring episodes of oral ulcers as well as ocular, genital, and skin lesions. Oral ulcers can affect tooth brushing and impair proper oral hygiene. As a result, a dental biofilm accumulates, and the condition of the teeth and periodontal tissue deteriorates. The aim of this case report is to highlight the efficacy of periodontal treatment for patients with Behcet's disease. A 51-year-old man with Behcet's disease presented with generalized severe periodontitis. After basic treatment of the periodontal tissues, periodontal surgery was performed at several sites with bony defects. However, the patient developed severe stomatitis in the oral mucosa and gingiva after periodontal surgery. Administration of the antimicrobial agent cefdinir had little effect on recovery; however, subsequent administration of sitafloxacin resulted in significant improvement of the stomatitis. This case demonstrates that periodontal therapy is very useful for alleviating the oral signs and symptoms of Behcet's disease. Systemic antibiotic treatment with sitafloxacin (but not cefdinir) and mechanical debridement were effective in preventing the recurrence of aphthous ulcer outbreaks after periodontal surgery.

  • Functional Role of Lacrimal Gland Fibroblasts in a Mouse Model of Chronic Graft-Versus-Host Disease

    Yamane, M., Ogawa, Y., Mukai, S., Yaguchi, S., Kamijuku, H., Inaba, T., Asai, K., Morikawa, S., Kawakami, Y., Shimmura, S. and Tsubota, K.

    Cornea (Cornea)  37 ( 1 ) 102 - 108 2018.01

    ISSN  1536-4798

     View Summary

    PURPOSE: This study aimed to clarify the mechanisms and assess the characteristics of the chronic graft-versus-host disease (cGVHD) fibrosis in the lacrimal gland (LG) of mice. METHODS: Histopathology of LG tissues was examined by immunohistochemistry and electron microscopy. Cultured fibroblasts derived from the LG were analyzed by phase-contrast microscopy, immunocytochemistry, flow cytometry, proliferation assay, and invasion and migration assays. RESULTS: Cultured murine LG fibroblasts in cGVHD were spindle-shaped and relatively small, whereas those from syngeneic controls were polygon-shaped and relatively large. Flow cytometric analysis showed that the LG fibroblasts in cGVHD had elevated HSP47 levels. The LG fibroblasts in cGVHD also showed increased expression of major histocompatibility complex class II. Furthermore, the proportion of Sca-1PDGFR-alpha cells among the LG fibroblasts in cGVHD was considerably increased compared with controls. Cell counting kit-8 assays demonstrated that the LG fibroblasts in cGVHD were highly proliferative, and cell invasion assays indicated that they were highly invasive with high migration ability. CONCLUSIONS: LG fibroblasts in cGVHD can be aberrantly activated, thereby eliciting fibrosis by producing excessive extracellular matrix, leading to LG dysfunction in mice.

  • The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry

    Niibe, K., Zhang, M., Nakazawa, K., Morikawa, S., Nakagawa, T., Matsuzaki, Y. and Egusa, H.

    Jpn Dent Sci Rev 53 ( 2 ) 25 - 33 2017.05

    ISSN  1882-7616

     View Summary

    Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRalpha(+) and Sca-1(+)) and humans (LNGFR(+), THY-1(+) and VCAM-1(+)), facilitating the isolation of unique enriched BM-MSCs (so-called "purified MSCs"). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.

  • Isolation of dental pulp stem cells with high osteogenic potential

    Yasui, T., Mabuchi, Y., Morikawa, S., Onizawa, K., Akazawa, C., Nakagawa, T., Okano, H. and Matsuzaki, Y.

    Inflamm Regen 37 ( April ) 8 2017.04

    ISSN  1880-9693

     View Summary

    Dental pulp stem cells/progenitor cells (DPSCs) can be easily obtained and can have excellent proliferative and mineralization potentials. Therefore, many studies have investigated the isolation and bone formation of DPSCs. In most previous reports, human DPSCs were traditionally isolated by exploiting their ability to adhere to plastic tissue culture dishes. DPSCs isolated by plastic adherence are frequently contaminated by other cells, which limits the ability to investigate their basic biology and regenerative properties. Additionally, the proliferative and osteogenic potentials vary depending on the isolated cells. It is very difficult to obtain cells of a sufficient quality to elicit the required effect upon transplantation. Considering clinical applications, stem cells used for regenerative medicine need to be purified in order to increase the efficiency of bone regeneration, and a stable supply of these cells must be generated. Here, we review the purification of DPSCs and studies of cranio-maxillofacial bone regeneration using these cells. Additionally, we introduce the prospective isolation of DPSCs using specific cell surface markers: low-affinity nerve growth factor and thymocyte antigen 1.

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Papers, etc., Registered in KOARA 【 Display / hide

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Presentations 【 Display / hide

  • Full-mouth disinfection, systemic antibacterial therapy, and regenerative periodontal therapy for generalized aggressive periodontitis: a case report

    Morikawa S, Ouchi T, Nakayama R, Nakagawa T.



    Poster presentation, European Federation of Periodontlogy

  • Identification of mesenchymal stem cells with bright expression of LNGFR and THY-1 in neural crest-like cells derived from human iPS cells

    Ouchi T, Morikawa S, Nakagawa, T.



    Poster presentation, European Federation of Periodontlogy

  • A case report of severe chronic periodontitis with Amlodipine-induced gingival overgrowth

    Nakayama R, Morikawa, S, Nakagawa T.



    Poster presentation, European Federaration of Periodontlogy

  • LNGFR(+)THY-1(+) multipotent stem cells derived from human induced pluripotent stem cells

    Ouchi T, Shibata S, Kimura H, Nagoshi N, Fujimura Y, Morikawa, S, Sato K, Kawana H, Fukuda K, Nakamura M, Nakagawa T, Okano H.

    18th International Congress of Developmental Biology (Singapore) , 


    Poster presentation

  • LNGFR+THY-1+ neural crest like cells have potential of mesenchymal stem cells

    Ouchi T, Morikawa S, Nakagawa T.

    102nd Annual Meeting of the American Academy of Periodontology (San Diego) , 


    Poster presentation, American Academy of Periodontology

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Research Projects of Competitive Funds, etc. 【 Display / hide

  • Development of a CD73(+)MSCs-integrated functional periodontitis and peri-implantitis antimicrobial reconstruction device


    MEXT,JSPS, Grant-in-Aid for Scientific Research, 基盤研究(C), Principal investigator

  • Development of novel bone replacement material using PDGFRa(+) mesenchymal stem cells migration system


    MEXT,JSPS, Grant-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

Intellectual Property Rights, etc. 【 Display / hide

  • ヒトiPS細胞から、ヒト歯原性上皮細胞やヒト歯原性間葉細胞を製造する方法

    Date applied: P2016-073957  2016.04 

    Patent, Joint

  • ヒト間葉系幹細胞濃縮方法

    Date applied: PCT/JP2008/066169 (USA 12/676,827)  2008.09 

    Patent, Joint

  • ヒト間葉系幹細胞濃縮方法

    Date applied: P2007-231298  2007.09 

    Patent, Joint

  • 分化細胞由来多能性幹細胞の樹立方法

    Date applied: 2009181009   

    Patent, Joint

Awards 【 Display / hide

  • 2010年11月 慶應義塾大学医学部三四会奨励賞


  • 第9回日本再生医療学会総会 Young Investigator’s Award(若手研究奨励賞)



Courses Taught 【 Display / hide











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Courses Previously Taught 【 Display / hide

  • Dentistry

    Keio University


    Autumn Semester, Lecture

  • Dentistry

    Keio University




Memberships in Academic Societies 【 Display / hide

  • Japanese Society of Periodontology

  • Japanese Society of Oral and Maxillofacial Surgery

  • Japanese Stomatological Society

  • Japanese Society of Regenerative Medicine

  • Japanese Society of oral Implantology