KEIO RESEARCHERS INFORMATION SYSTEM

Career 【 Display / hide 】

Career 【 Display / hide 】

• 2003.04

  -

  2005.03

  慶應義塾大学医学部 研修医（歯科・口腔外科）

• 2009.04

  -

  2010.06

  独立行政法人国立病院機構栃木病院（栃木医療センター） 歯科口腔外科

• 2010.07

  -

  2017.09

  慶應義塾大学医学部歯科・口腔外科学教室 助教

• 2011.04

  -

  Present

  Department of Dentistry and Oral Surgery, Keio University School of Medicine

• 2011.04

  -

  Present

  Department of Dentistry and Oral Surgery, Keio University School of Medicine

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Academic Background 【 Display / hide 】

Academic Background 【 Display / hide 】

• 2005.04

  -

  2009.03

  Keio University, 医学研究科博士課程

  Graduate School, Completed

Academic Degrees 【 Display / hide 】

Academic Degrees 【 Display / hide 】

• 博士（医学）, 慶應義塾大学大, Coursework

Research Areas 【 Display / hide 】

Research Areas 【 Display / hide 】

• Life Science / Regenerative dentistry and dental engineering

• Life Science / Regenerative dentistry and dental engineering

Research Keywords 【 Display / hide 】

Research Keywords 【 Display / hide 】

• Oral cancer

• Oral cancer

• Oral science

• Stem cells

• Stem cells

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Papers 【 Display / hide 】

Papers 【 Display / hide 】

• Recurrent Spindle Cell Carcinoma Shows Features of Mesenchymal Stem Cells

  Ouchi, T., Morikawa, S., Shibata, S., Takahashi, M., Yoshikawa, M., Soma, T., Miyashita, H., Muraoka, W., Kameyama, K., Kawana, H., Arima, Y., Saya, H., Okano, H., Nakagawa, T. and Asoda, S.

  J Dent Res (SAGE Publications Inc.)  97 ( 7 ) 779 - 786 2018.07

  Accepted,  ISSN  1544-0591

  　View Summary

  This study investigated a case of spindle cell carcinoma (SpCC) in tongue pathological lesions. The patient experienced a local recurrence and distant metastasis after surgical intervention. Although standard chemotherapy was administered, a granulomatous mass continued to develop. This aggressive growth led to survival of the tumor. Secondary debulking surgery was performed to improve the patient's quality of life at the request of the patient. Using a tissue sample derived from the secondary debulking surgery, we performed an analysis of the tumor's cell surface antigens, differentiation potential, metastatic ability, and inhibition potential by anticancer reagents. In vitro analysis revealed that the cell population grown under adherent culture conditions expressed the mesenchymal stem cell (MSC) markers CD73, CD90, and CD105. The cell line established from this SpCC contained colony-forming unit fibroblasts (CFU-Fs) and exhibited multipotent differentiation into several mesenchymal lineages, including bone, cartilage, and fat. The SpCC cells also displayed vigorous mobilization. These characteristics suggested that they had the differentiation potential of mesenchymal cells, especially MSCs, rather than that of epithelial cells. The surgical specimen analyzed in this study resisted the molecular target reagent cetuximab, which is an epidermal growth factor receptor inhibitor. This clinical insight revealed that chemotherapy-resistant SpCC cells have different characteristics compared to most other cancer cells, which are sensitive to cetuximab. Our cell death assay revealed that SpCC cell death was induced by the anticancer drug imatinib, which is known to inhibit protein tyrosine kinase activity of ABL, platelet-derived growth factor receptor alpha (PDGFRalpha), and KIT. Here, we report recurrent SpCC with characteristics of MSCs and potential for treatment with imatinib.

  • Access to Document (DOI)

• Treatment of severe generalized chronic periodontitis in a patient with Behcet's disease: A case report

  Morikawa, S., Ouchi, T., Asoda, S., Horie, N., Tsunoda, K., Kawana, H. and Nakagawa, T.

  J Int Med Res (Journal of International Medical Research)  46 ( 5 ) 2037 - 2045 2018.05

  ISSN  1473-2300

  　View Summary

  Behcet's disease is a systemic disorder of unknown etiology. It involves multiple organ systems and is characterized by recurring episodes of oral ulcers as well as ocular, genital, and skin lesions. Oral ulcers can affect tooth brushing and impair proper oral hygiene. As a result, a dental biofilm accumulates, and the condition of the teeth and periodontal tissue deteriorates. The aim of this case report is to highlight the efficacy of periodontal treatment for patients with Behcet's disease. A 51-year-old man with Behcet's disease presented with generalized severe periodontitis. After basic treatment of the periodontal tissues, periodontal surgery was performed at several sites with bony defects. However, the patient developed severe stomatitis in the oral mucosa and gingiva after periodontal surgery. Administration of the antimicrobial agent cefdinir had little effect on recovery; however, subsequent administration of sitafloxacin resulted in significant improvement of the stomatitis. This case demonstrates that periodontal therapy is very useful for alleviating the oral signs and symptoms of Behcet's disease. Systemic antibiotic treatment with sitafloxacin (but not cefdinir) and mechanical debridement were effective in preventing the recurrence of aphthous ulcer outbreaks after periodontal surgery.

  • Access to Document (DOI)

• Functional Role of Lacrimal Gland Fibroblasts in a Mouse Model of Chronic Graft-Versus-Host Disease

  Yamane, M., Ogawa, Y., Mukai, S., Yaguchi, S., Kamijuku, H., Inaba, T., Asai, K., Morikawa, S., Kawakami, Y., Shimmura, S. and Tsubota, K.

  Cornea (Cornea)  37 ( 1 ) 102 - 108 2018.01

  Accepted,  ISSN  1536-4798

  　View Summary

  PURPOSE: This study aimed to clarify the mechanisms and assess the characteristics of the chronic graft-versus-host disease (cGVHD) fibrosis in the lacrimal gland (LG) of mice. METHODS: Histopathology of LG tissues was examined by immunohistochemistry and electron microscopy. Cultured fibroblasts derived from the LG were analyzed by phase-contrast microscopy, immunocytochemistry, flow cytometry, proliferation assay, and invasion and migration assays. RESULTS: Cultured murine LG fibroblasts in cGVHD were spindle-shaped and relatively small, whereas those from syngeneic controls were polygon-shaped and relatively large. Flow cytometric analysis showed that the LG fibroblasts in cGVHD had elevated HSP47 levels. The LG fibroblasts in cGVHD also showed increased expression of major histocompatibility complex class II. Furthermore, the proportion of Sca-1PDGFR-alpha cells among the LG fibroblasts in cGVHD was considerably increased compared with controls. Cell counting kit-8 assays demonstrated that the LG fibroblasts in cGVHD were highly proliferative, and cell invasion assays indicated that they were highly invasive with high migration ability. CONCLUSIONS: LG fibroblasts in cGVHD can be aberrantly activated, thereby eliciting fibrosis by producing excessive extracellular matrix, leading to LG dysfunction in mice.

  • Access to Document (DOI)

• The potential of enriched mesenchymal stem cells with neural crest cell phenotypes as a cell source for regenerative dentistry

  Niibe, K., Zhang, M., Nakazawa, K., Morikawa, S., Nakagawa, T., Matsuzaki, Y. and Egusa, H.

  Jpn Dent Sci Rev 53 ( 2 ) 25 - 33 2017.05

  Accepted,  ISSN  1882-7616

  　View Summary

  Effective regenerative treatments for periodontal tissue defects have recently been demonstrated using mesenchymal stromal/stem cells (MSCs). Furthermore, current bioengineering techniques have enabled de novo fabrication of tooth-perio dental units in mice. These cutting-edge technologies are expected to address unmet needs within regenerative dentistry. However, to achieve efficient and stable treatment outcomes, preparation of an appropriate stem cell source is essential. Many researchers are investigating the use of adult stem cells for regenerative dentistry; bone marrow-derived MSCs (BM-MSCs) are particularly promising and presently used clinically. However, current BM-MSC isolation techniques result in a heterogeneous, non-reproducible cell population because of a lack of identified distinct BM-MSC surface markers. Recently, specific subsets of cell surface markers for BM-MSCs have been reported in mice (PDGFRalpha(+) and Sca-1(+)) and humans (LNGFR(+), THY-1(+) and VCAM-1(+)), facilitating the isolation of unique enriched BM-MSCs (so-called "purified MSCs"). Notably, the enriched BM-MSC population contains neural crest-derived cells, which can differentiate into cells of neural crest- and mesenchymal lineages. In this review, characteristics of the enriched BM-MSCs are outlined with a focus on their potential application within future regenerative dentistry.

  • Access to Document (DOI)

• Isolation of dental pulp stem cells with high osteogenic potential

  Yasui, T., Mabuchi, Y., Morikawa, S., Onizawa, K., Akazawa, C., Nakagawa, T., Okano, H. and Matsuzaki, Y.

  Inflamm Regen 37 ( April ) 8 - 10 2017.04

  Accepted,  ISSN  1880-9693

  　View Summary

  Dental pulp stem cells/progenitor cells (DPSCs) can be easily obtained and can have excellent proliferative and mineralization potentials. Therefore, many studies have investigated the isolation and bone formation of DPSCs. In most previous reports, human DPSCs were traditionally isolated by exploiting their ability to adhere to plastic tissue culture dishes. DPSCs isolated by plastic adherence are frequently contaminated by other cells, which limits the ability to investigate their basic biology and regenerative properties. Additionally, the proliferative and osteogenic potentials vary depending on the isolated cells. It is very difficult to obtain cells of a sufficient quality to elicit the required effect upon transplantation. Considering clinical applications, stem cells used for regenerative medicine need to be purified in order to increase the efficiency of bone regeneration, and a stable supply of these cells must be generated. Here, we review the purification of DPSCs and studies of cranio-maxillofacial bone regeneration using these cells. Additionally, we introduce the prospective isolation of DPSCs using specific cell surface markers: low-affinity nerve growth factor and thymocyte antigen 1.

  • Access to Document (DOI)

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Papers, etc., Registered in KOARA 【 Display / hide 】

Papers, etc., Registered in KOARA 【 Display / hide 】

• Development of a method to prevent osteonecrosis of the jaw using organoids derived from dental pulp stem cells

  Morikawa, Satoru

  科学研究費補助金研究成果報告書 2023

• Development of novel bone replacement material using PDGFRa(+) mesenchymal stem cells migration system

  Morikawa, Satoru

  科学研究費補助金研究成果報告書 2022

• Identification of targetable genetic mutations and establishment of personalized therapy in standard therapy-resistant oral squamous cell carcinoma

  Morikawa, Satoru

  科学研究費補助金研究成果報告書 2022

• Practical periodontal tissue regeneration therapy using highly purified dental pulp MSCs, induced neural crest cells, and induced odontogenic mesenchymal cells

  Morikawa, Satoru

  科学研究費補助金研究成果報告書 2020

• Application of purfied dental pulp stem cells for treatment of osteonecrosis of the jaw

  Morikawa, Satoru

  科学研究費補助金研究成果報告書 2019

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Reviews, Commentaries, etc. 【 Display / hide 】

Reviews, Commentaries, etc. 【 Display / hide 】

• 重度慢性歯周炎(限局型Stage III Grade C)患者に対し歯周組織再生療法を行なった一症例

  古澤春佳, 森川暁, 中川種昭

  日本歯周病学会会誌(Web) 67 2025

  Lead author, Last author, Corresponding author,  ISSN  1880-408X

• Functional Analysis of PDGFRα-positive Mesenchymal Stem/Stromal Cells in the Regulation of In Vivo Tissue Formation: Potential for Therapeutic Applications

  森川暁

  日本再生医療学会総会(Web) 24th 2025

  Lead author, Last author, Corresponding author

• Methods for creating periodontitis model mice: Their evolution and application

  森川暁, 加瀬義高, 岡野栄之

  月刊細胞 57 ( 10 )  2025

  Lead author, Last author, Corresponding author,  ISSN  1346-7557

• 広汎型重度慢性歯周炎患者に対して歯周組織再生療法と切除療法を行なった一症例

  浅野崇浩, 森川暁, 中川種昭

  日本歯周病学会会誌(Web) 67 2025

  Lead author, Last author, Corresponding author,  ISSN  1880-408X

• サイトランスグラニュールの歯周領域における新たな適応方法の可能性~間葉系幹細胞・間質細胞からみた結合組織移植片の新たな可能性~

  森川暁

  日本歯周病学会会誌(Web) 67 2025

  Lead author, Last author, Corresponding author,  ISSN  1880-408X

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Presentations 【 Display / hide 】

Presentations 【 Display / hide 】

• 口腔内慢性炎症が誘発する神経炎症

  森川暁

  第46回日本炎症・再生医学会 シンポジウム3 神経炎症と修復の最前線, 

  2025.07

  -

  2025.08

  , 

  Symposium, workshop panel (nominated)

• サイトランスグラニュールの歯周領域における新たな適応方法の可能性～間葉系幹細胞・間質細胞からみた結合組織移植片の新たな可能性～

  森川暁

  第68回春季日本歯周病学会学術大会 ランチョンセミナー6 最新の歯周組織再生療法における外科手技とサイトランスグラニュールの有効性, 

  2025.05

  , 

  Symposium, workshop panel (nominated)

• Connective Tissue Graft as a Potential Reservoir of Mesenchymal Stem/Stromal Cells

  Morikawa, S., Katayama, A., Nakagawa, T.

  EuroPerio11 (11TH CONFERENCE OF THE EUROPEAN FEDERATION OF PERIODONTOLOGY), 

  2025.05

• Optimizing Interdental Papilla Incision Design in Molar Periodontal Regenerative Therapy

  Shibazaki, S., Morikawa, S., Nakagawa, T.

  EuroPerio11 (11TH CONFERENCE OF THE EUROPEAN FEDERATION OF PERIODONTOLOGY), 

  2025.05

• Double-sided Entire Papilla Preservation Technique for Periodontal Regenerative Therapy

  Tanaka, K., Morikawa, S., Nakagawa, T.

  EuroPerio11 (11TH CONFERENCE OF THE EUROPEAN FEDERATION OF PERIODONTOLOGY), 

  2025.05

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Research Projects of Competitive Funds, etc. 【 Display / hide 】

Research Projects of Competitive Funds, etc. 【 Display / hide 】

• Elucidation of Multi-organ Frailty Caused by Periodontitis-Induced Inflammaging and Development of Preventive Strategies

  2025.04

  -

  2028.03

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Principal investigator

• 純化歯髄幹細胞と血管内皮細胞由来オルガノイドによる顎骨壊死予防

  2024.04

  -

  2028.03

  日本学術振興会, 科学研究費助成事業, 基盤研究(C), No Setting

• Connective Tissue Graft Mesenchymal Stem Cell Layer and Jaw Bone Marrow-Derived Hematopoietic Stem Cells Therapeutic Development Research

  2024.04

  -

  2027.03

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), No Setting

• 1. Research on the relationship between oral and systemic diseases Development of novel therapies using stem cells Study on the effects of oral microbiota on tumorigenesis

  2024.04

  -

  2026.03

  ONUKI KIKIN, Principal investigator

• Functional analysis of inflammation-inducing bacteria derived form oral microflora and immunoreactive T cells in gastrointestinal cancer

  2023.04

  -

  2026.03

  Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), No Setting

  　View Summary

  本研究では、口腔内マイクロバイオーム由来の炎症誘導性細菌の同定と制御性T細胞（regulatory T cell, Treg）の機能解析を行い消化器癌の進展における関与を解明することを目的とする。
  歯周病モデルの作製について、本研究では、C3H/HeNJclおよびDBA/2JJC1実験マウスを用いて縫合糸を用いた歯頸部結紮法により歯周病感染を誘導する実験を行った。歯周病モデルにおける腫瘍の生着と増殖の検証について、歯頸部結紮法により歯周病を誘導したC3H/HeNJclおよびDBA/2JJC1マウスを用いてマウス由来の腫瘍細胞であるLM8およびKLN205をそれぞれ背部皮下に105cells移植を行い腫瘍細胞の生着と増殖の観察を行った。腫瘍細胞の移植４週間後に背部に形成された腫瘍組織の重量を計測した結果、歯周病群において腫瘍形成が亢進している傾向が確認された。
  次に、潰瘍性大腸炎関連大腸癌と歯周病の関連については、潰瘍性大腸炎関連大腸癌（UC関連大腸癌）は大腸での炎症を背景として発生する癌である。今回、UCマウスモデルとして良く用いられているデキストラン硫酸ナトリウム（DSS）誘発性炎症性腸疾患モデルを用いて、歯周病が腸内の炎症に及ぼす影響を検討した。その結果、歯周病を起こしたマウスではDSSによる炎症がより強く起こることが分かった。このことは、歯周病が腸炎を増悪させ、UC関連癌発生リスクを上昇させる可能性を示唆した。今後は炎症を惹起させる原因となっている免疫細胞に着目し、さらなるメカニズム解明に繋げる予定である。

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Intellectual Property Rights, etc. 【 Display / hide 】

Intellectual Property Rights, etc. 【 Display / hide 】

• ヒトiPS細胞から、ヒト歯原性上皮細胞やヒト歯原性間葉細胞を製造する方法

  Date applied： P2016-073957  2016.04 

  Patent, Joint

• ヒト間葉系幹細胞濃縮方法

  Date applied： PCT/JP2008/066169 (USA 12/676,827)  2008.09 

  Patent, Joint

• ヒト間葉系幹細胞濃縮方法

  Date applied： P2007-231298  2007.09 

  Patent, Joint

• 分化細胞由来多能性幹細胞の樹立方法

  Date applied： 2009181009   

  Patent, Joint

Awards 【 Display / hide 】

Awards 【 Display / hide 】

• 2010年11月　慶應義塾大学医学部三四会奨励賞

  2010.11

• 2010年11月 慶應義塾大学医学部三四会奨励賞

  2010.11

• 第9回日本再生医療学会総会　Young Investigator’s Award（若手研究奨励賞）

  2010.03

• 第9回日本再生医療学会総会 Young Investigator’s Award（若手研究奨励賞）

  2010.03

Courses Taught 【 Display / hide 】

Courses Taught 【 Display / hide 】

• DENTISTRY AND ORAL SURGERY

  2026

• DENTISTRY AND ORAL SURGERY: PRACTICE

  2026

• LECTURE SERIES, DENTISTRY

  2026

• DENTISTRY AND ORAL SURGERY: SEMINAR

  2026

• ADVANCED DENTISTRY AND ORAL SURGERY

  2026

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Courses Previously Taught 【 Display / hide 】

Courses Previously Taught 【 Display / hide 】

• LECTURE SERIES, DENTISTRY

  Keio University

  2026.04

  -

  2027.03

• ADVANCED DENTISTRY AND ORAL SURGERY

  Keio University

  2026.04

  -

  2027.03

• DENTISTRY AND ORAL SURGERY: SEMINAR

  Keio University

  2026.04

  -

  2027.03

• DENTISTRY AND ORAL SURGERY: PRACTICE

  Keio University

  2026.04

  -

  2027.03

• DENTISTRY AND ORAL SURGERY

  Keio University

  2026.04

  -

  2027.03

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Memberships in Academic Societies 【 Display / hide 】

Memberships in Academic Societies 【 Display / hide 】

• Japanese Society of Periodontology

  　

• Japanese Society of Oral and Maxillofacial Surgery

  　

• Japanese Stomatological Society

  　

• Japanese Society of Regenerative Medicine

  　

• Japanese Society of oral Implantology