Tanaka, Kenji



School of Medicine, Division of Brain Sciences (Shinanomachi)



E-mail Address

E-mail address

Related Websites

External Links

Profile 【 Display / hide

  • 1991年 麻布高校卒
    1997年 慶應義塾大学医学部卒
    2003年 慶應義塾大学大学院修了
    2016年 慶應義塾大学医学部精神神経科学 准教授
    2019年 慶應義塾大学体育会アメリカンフットボール部 部長

Message from the Faculty Member 【 Display / hide

  • patience

Academic Degrees 【 Display / hide

  • Doctor (Medicine), Keio University, Coursework, 2003.03

Licenses and Qualifications 【 Display / hide

  • 医師免許, 1997.04


Research Areas 【 Display / hide

  • Neurochemistry/Neuropharmacology

Research Keywords 【 Display / hide

  • FASTシステム

  • KENGE-tet

  • fiberphotometry

  • optogenetics

  • motivation

display all >>


Papers 【 Display / hide

  • Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice

    Miyata S., Kakizaki T., Fujihara K., Obinata H., Hirano T., Nakai J., Tanaka M., Itohara S., Watanabe M., Tanaka K.F., Abe M., Sakimura K., Yanagawa Y.

    Molecular Brain (Molecular Brain)  14 ( 1 ) 5 2021.12

     View Summary

    Reduced expression of glutamate decarboxylase 67 (GAD67), encoded by the Gad1 gene, is a consistent finding in postmortem brains of patients with several psychiatric disorders, including schizophrenia, bipolar disorder and major depressive disorder. The dysfunction of GAD67 in the brain is implicated in the pathophysiology of these psychiatric disorders; however, the neurobiological consequences of GAD67 dysfunction in mature brains are not fully understood because the homozygous Gad1 knockout is lethal in newborn mice. We hypothesized that the tetracycline-controlled gene expression/suppression system could be applied to develop global GAD67 knockdown mice that would survive into adulthood. In addition, GAD67 knockdown mice would provide new insights into the neurobiological impact of GAD67 dysfunction. Here, we developed Gad1tTA/STOP−tetO biallelic knock-in mice using Gad1STOP−tetO and Gad1tTA knock-in mice, and compared them with Gad1+/+ mice. The expression level of GAD67 protein in brains of Gad1tTA/STOP−tetO mice treated with doxycycline (Dox) was decreased by approximately 90%. The GABA content was also decreased in the brains of Dox-treated Gad1tTA/STOP−tetO mice. In the open-field test, Dox-treated Gad1tTA/STOP−tetO mice exhibited hyper-locomotor activity and decreased duration spent in the center region. In addition, acoustic startle responses were impaired in Dox-treated Gad1tTA/STOP−tetO mice. These results suggest that global reduction in GAD67 elicits emotional abnormalities in mice. These GAD67 knockdown mice will be useful for elucidating the neurobiological mechanisms of emotional abnormalities, such as anxiety symptoms associated with psychiatric disorders.

  • Flexible annotation atlas of the mouse brain: combining and dividing brain structures of the Allen Brain Atlas while maintaining anatomical hierarchy

    Takata N., Sato N., Komaki Y., Okano H., Tanaka K.F.

    Scientific Reports (Scientific Reports)  11 ( 1 ) 6234 2021.12

     View Summary

    A brain atlas is necessary for analyzing structure and function in neuroimaging research. Although various annotation volumes (AVs) for the mouse brain have been proposed, it is common in magnetic resonance imaging (MRI) of the mouse brain that regions-of-interest (ROIs) for brain structures (nodes) are created arbitrarily according to each researcher’s necessity, leading to inconsistent ROIs among studies. One reason for such a situation is the fact that earlier AVs were fixed, i.e. combination and division of nodes were not implemented. This report presents a pipeline for constructing a flexible annotation atlas (FAA) of the mouse brain by leveraging public resources of the Allen Institute for Brain Science on brain structure, gene expression, and axonal projection. A mere two-step procedure with user-specified, text-based information and Python codes constructs FAA with nodes which can be combined or divided objectively while maintaining anatomical hierarchy of brain structures. Four FAAs with total node count of 4, 101, 866, and 1381 were demonstrated. Unique characteristics of FAA realized analysis of resting-state functional connectivity (FC) across the anatomical hierarchy and among cortical layers, which were thin but large brain structures. FAA can improve the consistency of whole brain ROI definition among laboratories by fulfilling various requests from researchers with its flexibility and reproducibility.

  • Mice with reduced glutamate transporter GLT1 expression exhibit behaviors related to attention-deficit/hyperactivity disorder.

    Hiraoka Y, Sugiyama K, Nagaoka D, Tsutsui-Kimura I, Tanaka KF, Tanaka K

    Biochemical and biophysical research communications 567   161 - 165 2021.08

    ISSN  0006-291X

  • Chronic social defeat stress impairs goal-directed behavior through dysregulation of ventral hippocampal activity in male mice

    Yoshida K., Drew M.R., Kono A., Mimura M., Takata N., Tanaka K.F.

    Neuropsychopharmacology (Neuropsychopharmacology)  46 ( 9 ) 1606 - 1616 2021.08

    ISSN  0893133X

     View Summary

    Chronic stress is a risk factor for a variety of psychiatric disorders, including depression. Although impairments to motivated behavior are a major symptom of clinical depression, little is known about the circuit mechanisms through which stress impairs motivation. Furthermore, research in animal models for depression has focused on impairments to hedonic aspects of motivation, whereas patient studies suggest that impairments to appetitive, goal-directed motivation contribute significantly to motivational impairments in depression. Here, we characterized goal-directed motivation in repeated social defeat stress (R-SDS), a well-established mouse model for depression in male mice. R-SDS impaired the ability to sustain and complete goal-directed behavior in a food-seeking operant lever-press task. Furthermore, stress-exposed mice segregated into susceptible and resilient subpopulations. Interestingly, susceptibility to stress-induced motivational impairments was unrelated to stress-induced social withdrawal, another prominent effect of R-SDS in mouse models. Based on evidence that ventral hippocampus (vHP) modulates sustainment of goal-directed behavior, we monitored vHP activity during the task using fiber photometry. Successful task completion was associated with suppression of ventral hippocampal neural activity. This suppression was diminished after R-SDS in stress-susceptible but not stress-resilient mice. The serotonin selective reuptake inhibitor (SSRI) escitalopram and ketamine both normalized vHP activity during the task and restored motivated behavior. Furthermore, optogenetic vHP inhibition was sufficient to restore motivated behavior after stress. These results identify vHP hyperactivity as a circuit mechanism of stress-induced impairments to goal-directed behavior and a putative biomarker that is sensitive to antidepressant treatments and that differentiates susceptible and resilient individuals.

  • Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice.

    Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka KF

    Cell reports 36 ( 4 ) 109427 2021.07

display all >>

Papers, etc., Registered in KOARA 【 Display / hide

display all >>

Reviews, Commentaries, etc. 【 Display / hide

  • 新規小脳型多系統萎縮症モデルのCSF-1R阻害剤治療

    松瀬 大, 山口 浩雄, 眞崎 勝久, 西村 由宇慈, 田中 辰典, 雑賀 徹, 田中 謙二, 山崎 亮, 吉良 潤一, 磯部 紀子

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集 (Movement Disorder Society of Japan (MDSJ))  15回   92 - 92 2021.07

  • オリゴデンドロサイト特異的αシヌクレイン蓄積による小脳型多系統萎縮症モデルの樹立

    松瀬 大, 山口 浩雄, 眞崎 勝久, 西村 由宇慈, 田中 辰典, 雑賀 徹, 田中 謙二, 山崎 亮, 吉良 潤一

    パーキンソン病・運動障害疾患コングレスプログラム・抄録集 (Movement Disorder Society of Japan (MDSJ))  14回   110 - 110 2021.02

  • 新しい役者を標的にした脳卒中研究の新潮流-グリア細胞、神経幹細胞、血管壁細胞- 血管壁細胞の光操作

    田中 謙二, 阿部 欣史, 權 秀珍, 大石 光洋

    脳循環代謝 ((一社)日本脳循環代謝学会)  32 ( 1 ) 54 - 54 2020.11

    ISSN  0915-9401

  • COVID-19こころのケアチーム活動報告

    木村 範子, 河野 佐代子, 滝上 紘之, 山市 大輔, 片山 奈理子, 竹内 啓善, 菊地 俊暁, 佐渡 充洋, 内田 裕之, 田中 謙二, 三村 將

    総合病院精神医学 ((一社)日本総合病院精神医学会)  32 ( Suppl. ) S - 193 2020.11

    ISSN  0915-5872

  • 新規小脳型多系統萎縮症モデルによる脱髄・神経細胞障害機序とグリア制御による新規治療法開発の試み

    西村 由宇慈, 真崎 勝久, 松瀬 大, 山口 浩雄, 雑賀 徹, 田中 辰典, 田中 謙二, 山崎 亮, 貞島 祥子, 岩城 徹, 吉良 潤一

    神経免疫学 (日本神経免疫学会)  25 ( 1 ) 110 - 110 2020.10

    ISSN  0918-936X

display all >>

Presentations 【 Display / hide

  • the 5th BRI International Symposium: Genome editing technology; its status quo and application to brain research

    Tanaka Kenji

    the 5th BRI International Symposium: Genome editing technology; its status quo and application to brain research, 2015.03, Oral Presentation(general)

  • 特定細胞集団の活動を操作する、観察する

    Tanaka Kenji

    都医学研セミナー (東京都医学総合研究所 講堂) , 2015.01, Public discourse, seminar, tutorial, course, lecture and others, 東京都医学総合研究所

  • 第25回マイクロダイアリシス研究会

    Tanaka Kenji

    第25回マイクロダイアリシス研究会, 2014.12, Oral Presentation(general)

  • 精神医学研究にオプトジェネティクスが期待されること

    Tanaka Kenji

    第35回日本レーザー医学会総会, 2014.11, Oral Presentation(guest/special)

  • Microglia transform astrocytes into neuroprotective phenotypes by inhibiting P2Y1 receptors.

    Tanaka Kenji

    第57回日本神経化学会大会 (奈良県文化会館) , 2014.09, Oral Presentation(general)

display all >>

Research Projects of Competitive Funds, etc. 【 Display / hide

  • Glia decoding: deciphering information critical for brain-body interactions


    The University of Tokyo, 岡部 繁男, 星野 歩子, 松田 道行, 小泉 修一, 石井 優, 田中 謙二, 津田 誠, 史 蕭逸, 小山 隆太, 和氣 弘明, Grant-in-Aid for Transformative Research Areas (A)

     View Summary


  • グリア・神経ネットワークの統合による脳内エネルギー代謝機構


    Keio University, 田中 謙二, 松井 広, Grant-in-Aid for Transformative Research Areas (A)

     View Summary


  • 意欲行動の持続にかかわる神経基盤の解明


    MEXT,JSPS, Grant-in-Aid for Scientific Research, 田中 謙二, Grant-in-Aid for Scientific Research on Innovative Areas, Principal Investigator

     View Summary


  • Establishment and applications of model mice with fluctuated deafness by advanced gene-engineering methods


    Niigata University, 日比野 浩, 澤村 晴志朗, 増田 正次, 田中 謙二, 神崎 晶, 崔 森悦, 任 書晃, 永森 收志, Grant-in-Aid for Scientific Research (A)

     View Summary


  • グリアアセンブリによる脳機能発現の制御と病態


    Niigata University, 竹林 浩秀, 小泉 修一, 田中 謙二, 尾崎 紀夫, 岡部 繁男, 池中 一裕, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

     View Summary


display all >>

Works 【 Display / hide

  • 夏休みの研究体験


    Other, Single

     View Details


  • ゆとりの時間「Let's Enjoy High School Science」



    Other, Single

     View Details

    慶應義塾湘南藤沢高等部 1年生の生物選択の生徒対象 


Courses Taught 【 Display / hide











display all >>