論文 - 鳥居　秀成

Estimation of the minimum effective dose of dietary supplement crocetin for prevention of myopia progression in mice

K Mori, T Kurihara, X Jiang, S Ikeda, E Yotsukura, H Torii, K Tsubota

Nutrients 12 (1), 180 2020年

筆頭著者, 最終著者, 責任著者

Centrifugal Pump

H Torii, K Nakaya

US Patent App. 16/763,241 2020年

筆頭著者, 最終著者, 責任著者

Author Correction: Ocular biometry and refractive outcomes using two swept-source optical coherence tomography-based biometers with segmental or equivalent refractive indices

MK Omoto, H Torii, S Masui, M Ayaki, K Tsubota, K Negishi

Scientific reports 10 (1), 13181 2020年

筆頭著者, 最終著者, 責任著者

Implantation of a Toric Intraocular Lens after Several Radial Keratotomy Procedures: A Case Report

SS Chen, H Torii, E Yotsukura, Y Nishi, K Tsubota, K Negishi

2020 ASCRS Annual Meeting 2020年

筆頭著者, 最終著者, 責任著者

Subjective Happiness and Sleep in University Students with High Myopia

I Iehisa, K Negishi, R Sakamoto, H Torii, M Ayaki, K Tsubota

Psych 2 (4), 279-286 2020年

筆頭著者, 最終著者, 責任著者

Refractive stability of a new single-piece hydrophobic acrylic intraocular lens and corneal wound repair after implantation using a new automated intraocular lens delivery system.

Negishi K, Masui S, Torii H, Nishi Y, Tsubota K

PloS one （PLoS ONE）  15 （ 9 ） e0238366 2020年

筆頭著者, 最終著者, 責任著者,  ISSN  1932-6203

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Purpose To investigate refractive stability and characterize corneal incision repair up to 3 months after implantation of a new hydrophobic acrylic intraocular lens (IOL) with hydroxyethyl-methacrylate using a new automated IOL delivery system. Methods This prospective case series included 50 eyes of 50 patients undergoing phacoemulsification and implantation of the Clareon® CNA0T0 IOL using the AutonoMe® automated delivery system in the Department of Ophthalmology, Keio University School of Medicine. The clinical data were collected from 46 eyes of 46 patients preoperatively and 1 day, 1 week, and 1 and 3 months postoperatively. Endothelial-side incision gaping, posterior incision retraction, and Descemet’s membrane detachment were recorded as present or absent using anterior-segment optical coherence tomography postoperatively. Results The uncorrected distance and corrected distance visual acuities improved and stabilized 1 week postoperatively. The anterior chamber depth was stable from 1 week postoperatively. The subjective refraction was stable from 1 day postoperatively. Descemet’s membrane detachments and endothelial-side wound gaping were seen in 19 (41.3%) eyes and 34 (73.9%) eyes 1 day postoperatively and decreased gradually. Posterior incision retraction was seen in eight eyes (17.4%) on day 1 and increased to 19 eyes (41.3%) 3 months postoperatively. Conclusions The Clareon IOL had excellent refractive stability from day 1 postoperatively. The AutonoMe automated delivery system enables safe IOL implantation through a 2.4-mm corneal incision, although the wound required longer than 1 month to heal postoperatively.

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Optical element

S Shioya, K Tsubota, T Kurihara, H Torii

US Patent 10,866,433 2020年

筆頭著者, 最終著者, 責任著者

Ocular biometry and refractive outcomes using two swept-source optical coherence tomography-based biometers with segmental or equivalent refractive indices (vol 63, 251, 2020)

MK Omoto, H Torii, S Masui, M Ayaki, K Tsubota, K Negishi

SCIENTIFIC REPORTS 10 (1) 2020年

筆頭著者, 最終著者, 責任著者

Myopia treatment device

K Tsubota, K Negishi, H Torii, T Kurihara

US Patent 10,823,982 2020年

筆頭著者, 最終著者, 責任著者

Oral crocetin administration suppressed refractive shift and axial elongation in a murine model of lens-induced myopia

Mori K., Kurihara T., Miyauchi M., Ishida A., Jiang X., Ikeda S.i., Torii H., Tsubota K.

Scientific Reports （Scientific Reports）  9 （ 1 ） 295 2019年12月

ISSN  2045-2322

　概要を見る

© 2019, The Author(s). Increased global incidence of myopia necessitates establishment of therapeutic approaches against its progression. To explore agents which may control myopia, we screened 207 types of natural compounds and chemical reagents based on an activity of a myopia suppressive factor, early growth response protein 1 (Egr-1) in vitro. Among the candidates, crocetin showed the highest and dose-dependent activation of Egr-1. For in vivo analysis, experimental myopia was induced in 3-week-old C57BL/6 J mice with −30 diopter (D) lenses for 3 weeks. Animals were fed with normal or mixed chow containing 0.003% (n = 19) and 0.03% (n = 7) of crocetin during myopia induction. Refraction and axial length were measured at 3-week-old and the 6-week-old with an infrared photorefractor and a SD-OCT system. Compared to controls (n = 14), crocetin administration showed a significant smaller change of refractive errors (−13.62 ± 8.14 vs +0.82 ± 5.81 D for 0.003%, p < 0.01, −2.00 ± 4.52 D for 0.03%, p < 0.01) and axial elongation (0.27 ± 0.03 vs 0.22 ± 0.04 mm for 0.003%, p < 0.01, 0.23 ± 0.05 mm for 0.03%, p < 0.05). These results suggest that a dietary factor crocetin may have a preventive effect against myopia progression.

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Effects of hyperoxia on the refraction in murine neonatal and adult models

Mori K., Kurihara T., Jiang X., Ikeda S.I., Ishida A., Torii H., Tsubota K.

International Journal of Molecular Sciences （International Journal of Molecular Sciences）  20 （ 23 ）  2019年12月

ISSN  16616596

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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Whether hyperoxia affects the refraction in neonatal and adult mice is unknown. The mice exposed to 85% oxygen at postnatal 8 days (P8d) for 3 days and the mice exposed to normal air were assigned to the neonatal hyperoxia and normoxia groups, respectively. The refraction, the corneal curvature radius (CR) and the axial length (AL) were measured at P30d and P47d. Postnatal 6 weeks (P6w) adult mice were divided into the adult hyperoxia and normoxia groups. These parameters were measured before oxygen exposure, after 1 and 6 weeks, and every 7 weeks. The lens elasticity was measured at P7w and P26w by enucleation. The neonatal hyperoxia group showed a significantly larger myopic change than the neonatal normoxia group (P47d −6.56 ± 5.89 D, +4.11 ± 2.02 D, p < 0.001), whereas the changes in AL were not significantly different (P47d, 3.31 ± 0.04 mm, 3.31 ± 0.05 mm, p = 0.852). The adult hyperoxia group also showed a significantly larger myopic change (P12w, −7.20 ± 4.09 D, +7.52 ± 2.54 D, p < 0.001). The AL did not show significant difference (P12w, 3.44 ± 0.03 mm, 3.43 ± 0.01 mm, p = 0.545); however, the CR in the adult hyperoxia group was significantly smaller than the adult normoxia group (P12w, 1.44 ± 0.03 mm, 1.50 ± 0.03 mm, p = 0.003). In conclusion, hyperoxia was demonstrated to induce myopic shift both in neonatal and adult mice, which was attributed to the change in the CR rather than the AL. Elucidation of the mechanisms of hyperoxia and the application of this result to humans should be carried out in future studies.

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Ocular biometry and refractive outcomes using two swept-source optical coherence tomography-based biometers with segmental or equivalent refractive indices

Omoto M., Torii H., Masui S., Ayaki M., Tsubota K., Negishi K.

Scientific Reports （Scientific Reports）  9 （ 1 ） 6557 2019年12月

ISSN  2045-2322

　概要を見る

© 2019, The Author(s). This study compared the axial length (AL), central corneal thickness (CCT), anterior chamber depth (ACD), lens thickness (LT), mean anterior corneal radius of curvature (Rm), and postoperative refractive outcomes obtained from two different swept-source optical coherence biometers, the ARGOS (Movu, Nagoya, Japan), which uses the segmental refractive index for each segment, and the IOLMaster 700 (Carl Zeiss Meditec, Jena, Germany), which uses an equivalent refractive index for the entire eye. One hundred and six eyes of 106 patients with cataracts were included. The refractive outcomes using the Barrett Universal II, Haigis, Hoffer Q, and SRK/T formulas were evaluated. The mean AL, CCT, ACD, and Rm differed significantly (P < 0.001) with the IOLMaster 700 (25.22 mm, 559 µm, 3.23 mm, and 7.69 mm) compared with the ARGOS (25.14 mm, 533 µm, 3.33 mm, and 7.66 mm). The mean LTs did not differ significantly. The percentages of eyes within ±0.50 and ±1.00 diopter of the predicted refraction did not differ significantly (P > 0.05). The accuracy of the intraocular lens power calculations was clinically acceptable with both biometers, although the ocular biometry using these two biometers exhibited certain differences.

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Current Prevalence of Myopia and Association of Myopia with Environmental Factors among Schoolchildren in Japan

Yotsukura E., Torii H., Inokuchi M., Tokumura M., Uchino M., Nakamura K., Hyodo M., Mori K., Jiang X., Ikeda S., Kondo S., Negishi K., Kurihara T., Tsubota K.

JAMA Ophthalmology （JAMA Ophthalmology）  137 （ 11 ） 1233 - 1239 2019年11月

ISSN  21686165

　概要を見る

© 2019 American Medical Association. All rights reserved. Importance: Given the estimates of increasing prevalence of myopia, especially in Asia, it is important to determine the current prevalence of myopia among populations of schoolchildren in Japan. Objective: To investigate the current prevalence rate of myopia and the association between environmental factors and myopia in Japanese schoolchildren. Design, Setting, and Participants: This cross-sectional study assessed 1478 participants, including 726 elementary school students and 752 junior high school students, at 2 schools in Tokyo, Japan, who underwent eye examinations from April 1 to May 31, 2017, that included measurement of the refractive errors by autorefractometry with noncycloplegic refraction and ocular biometric factors. After excluding those who had been treated with atropine or orthokeratology (n = 11), had a history of eye disease (n = 2), had no parental consent (n = 41), and were absent (n = 8), 1416 schoolchildren were analyzed. Main Outcomes and Measures: The primary outcome was the prevalence of myopia and high myopia. Secondary outcomes were environmental factors that were associated with myopia. Results: A total of 1416 schoolchildren (mean [SD] age, 10.8 [2.7] years; 792 [55.9%] male) were studied. The prevalence rates of myopia (spherical equivalent ≤-0.5 diopters [D]) were 76.5% (95% CI, 73.4%-79.7%) among the elementary school students and 94.9% (95% CI, 93.3%-96.5%) among the junior high school students. The prevalence rates of high myopia (spherical equivalent ≤-6.0 D) were 4.0% (95% CI, 2.5%-5.4%) among the elementary school students and 11.3% (95% CI, 8.8%-13.7%) among the junior high school students. The prevalence rates of high myopia classified based on axial length of 26.0 mm or longer were 1.2% (95% CI, 0.4%-2.0%) among elementary school students and 15.2% (95% CI, 12.5%-17.8%) among junior high school students. Multiple regression analysis showed that higher-order aberrations and dry eye disease were associated with refractive error in elementary school students (spherical aberration: β = 6.152; 95% CI, 3.161-9.143; P <.001; dry eye disease: β = -0.626; 95% CI, -1.189 to -0.063; P =.03) and with axial length in junior high school students (spherical-like aberration: β = 26.546; 95% CI, 18.708-34.385; P <.001; dry eye disease: β = 0.354; 95% CI, 0.131-0.578; P <.002). Conclusions and Relevance: Although the use of noncycloplegic autorefraction with a cutoff of -0.50 D could lead to overestimation of results, these findings suggest that the current prevalence rates of myopia among elementary and junior high school students in Asia are high, especially if the results from these 2 schools are generalizable to all schoolchildren in Japan and Asia..

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High Myopia and Its Associated Factors in JPHC-NEXT Eye Study: A Cross-Sectional Observational Study.

Mori K, Kurihara T, Uchino M, Torii H, Kawashima M, Sasaki M, Ozawa Y, Yamagishi K, Iso H, Sawada N, Tsugane S, Yuki K, Tsubota K

Journal of clinical medicine （Journal of Clinical Medicine）  8 （ 11 ）  2019年10月

筆頭著者, 最終著者, 責任著者,  ISSN  2077-0383

　概要を見る

The increasing prevalence of high myopia has been noted. We investigated the epidemiological characteristics and the related factors of high myopia in a Japanese adult population. Japan Public Health Center-Based Prospective Study for the Next Generation (JPHC-NEXT) Eye Study was performed in Chikusei-city, a rural area in mid-east Japan, between 2013 and 2015. A cross-sectional observational analysis was conducted to investigate prevalence and related factors of high myopia. A total of 6101 participants aged ≥40 years without a history of ocular surgeries was included. High myopia was defined as a spherical equivalent refraction of ≤−6.00 diopters according to the American Academy of Ophthalmology. Potential high myopia-related factors included intraocular pressure (IOP), corneal structure, corneal endothelial cell density, age, height, body mass index, heart rate, blood pressure, biochemical profile, and current history of systemic and ocular disorders. The odds ratios of high myopia were estimated using the logistic regression models adjusted for the associated factors. The prevalence of high myopia was 3.8% in males and 5.9% in females with a significant difference. Age was inversely associated, IOP was positively associated, and none of other factors were associated with high myopia in both sexes. In conclusion, only age and IOP were associated with high myopia in this community-based sample.

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Ultra-Widefield Retinal Imaging for Analyzing the Association Between Types of Pathological Myopia and Posterior Staphyloma.

Mimura R, Mori K, Torii H, Nagai N, Suzuki M, Minami S, Ozawa Y, Kurihara T, Tsubota K

Journal of clinical medicine （Journal of Clinical Medicine）  8 （ 10 ）  2019年09月

筆頭著者, 最終著者, 責任著者,  ISSN  2077-0383

　概要を見る

High myopia may develop to pathologic myopia, which brings severe visual impairment; however, the etiology is not fully understood. We, therefore, investigated the relationship between the presence of posterior staphyloma and posterior ocular disorders by assessing the patients with high myopia. A retrospective study was performed for the patients, who have more than 26 mm of the axial length and of whom fundus photography was taken with an ultra-widefield retinal imaging system. The objectives were 138 cases encompassing 229 eyes. In 138 cases, 91 were bilateral and 47 were unilateral. The averages ± SD of axial length of bilateral and unilateral were 28.8 ± 2.2 mm, 27.3 ± 1.2 mm, respectively, showing statistically significant difference. The number of eyes with and without posterior staphyloma were 107 (46.7%) and 122 (53.3%), respectively. Retinal detachment and retinal breaks are more observed in cases without posterior staphyloma (p = 0.017). Myopic choroidal neovascularization (mCNV) (p = 0.002), chorioretinal atrophy (p < 0.001), retinoschisis (p < 0.001), and optic neuropathy (p = 0.038) are more often seen in cases with posterior staphyloma. In conclusion, the prevalence rates of myopic choroidal neovascularization, retinal choroidal atrophy, and optic neuropathy were significantly higher with posterior staphyloma. The rate of periocular disorders such as retinal detachment was significantly higher without posterior staphyloma. These results indicate associations between types of pathological myopia and presence or absence of posterior staphyloma analyzed by ultra-widefield retinal imaging.

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連載 症例から学ぶ　白内障手術の実践レクチャー・術後編20 グレア・ハロー・dysphotopsia

鳥居 秀成

臨床眼科 （株式会社医学書院）  73 （ 9 ） 1122 - 1127 2019年09月

筆頭著者, 最終著者, 責任著者,  ISSN  03705579

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The Effect of Dietary Supplementation of Crocetin for Myopia Control in Children: A Randomized Clinical Trial.

Mori K, Torii H, Fujimoto S, Jiang X, Ikeda SI, Yotsukura E, Koh S, Kurihara T, Nishida K, Tsubota K

Journal of clinical medicine （Journal of Clinical Medicine）  8 （ 8 ）  2019年08月

筆頭著者, 最終著者, 責任著者,  ISSN  2077-0383

　概要を見る

The prevalence of myopia has been increasing in recent years. The natural carotenoid crocetin has been reported to suppress experimental myopia in mice. We evaluated the effects of crocetin on myopia suppression in children. A multicenter randomized double-blind placebo-controlled clinical trial was performed with 69 participants aged 6 to 12 years, whose cycloplegic spherical equivalent refractions (SER) were between −1.5 and −4.5 diopter (D). The participants were randomized to receive either a placebo or crocetin and followed up for 24 weeks. Axial length (AL) elongation and changes in SER were evaluated for 24 weeks. Both written informed assent from the participants and written informed consent from legal guardians were obtained in this study because the selection criteria of this trial included children aged between 6 and 12 years old. This trial was approved by the institutional review boards. A mixed-effects model was used for analysis, using both eyes. Two participants dropped out and 67 children completed this trial. The change in SER in the placebo group, −0.41 ± 0.05 D (mean ± standard deviation), was significantly more myopic compared to that in the crocetin group, −0.33 ± 0.05 D (p = 0.049). The AL elongation in the placebo group, 0.21 ± 0.02 mm, was significantly bigger than that in the crocetin group, 0.18 ± 0.02 mm (p = 0.046). In conclusion, dietary crocetin may have a suppressive effect on myopia progression in children, but large-scale studies are required in order to confirm this effect.

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Ocular-component-specific miRNA expression in a murine model of lens-induced myopia

Tanaka Y., Kurihara T., Hagiwara Y., Ikeda S., Mori K., Jiang X., Torii H., Tsubota K.

International Journal of Molecular Sciences （International Journal of Molecular Sciences）  20 （ 15 ）  2019年08月

ISSN  16616596

　概要を見る

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. To identify tissues and molecules involved in refractive myopic shift and axial length elongation in a murine lens-induced myopia model, we performed a comprehensive analysis of microRNA (miRNA) expression. Three weeks after negative 30 diopter lens fixation on three-week-old C57BL/6J mice, total RNA was extracted from individual ocular components including cornea, iris, lens, retina, retinal pigment epithelium (RPE)/choroid, and sclera tissue. The miRNA expression analysis was pooled from three samples and carried out using Agilent Mouse miRNA Microarray (8 × 60 K) miRBase21.0. The expression ratio was calculated, and differentially expressed miRNAs were extracted, using GeneSpring GX 14.5. Myopic induction showed a significant myopic refractive change, axial elongation, and choroidal thinning. Through the comprehensive miRNA analysis, several upregulated miRNAs (56 in cornea tissue, 13 in iris tissue, 6 in lens tissue, 0 in retina tissue, 29 in RPE/choroid tissue, and 30 in sclera tissue) and downregulated miRNAs (7 in cornea tissue, 28 in iris tissue, 17 in lens tissue, 9 in retina tissue, 7 in RPE/choroid tissue, and 40 in sclera tissue) were observed. Overlapping expression changes in miRNAs were also found in different ocular components. Some of this miRNA dysregulation may be functionally involved in refractive myopia shift and axial length elongation.

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Clinical Outcomes of KeraVio using Violet Light Emitting Glasses and Riboflavin for Corneal Ectasia: A Pilot Study

Kobashi Hidenaga, Torii Hidemasa, Toda Ikuko, Tsubota Kazuo

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 60 （ 9 ）  2019年07月

筆頭著者, 最終著者, 責任著者,  ISSN  0146-0404

Omega-3 polyunsaturated fatty acids suppressed experimental myopia progression in mice

Mori Kiwako, Kurihara Toshihide, Jiang Xiaoyan, Ikeda Shin-ichi, Arita Makoto, Torii Hidemasa, Tsubota Kazuo

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 60 （ 9 ）  2019年07月

筆頭著者, 最終著者, 責任著者,  ISSN  0146-0404